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Co-fitness analysis identifies a diversity of signal proteins involved in the utilization of specific c-type cytochromes 共适应度分析确定了参与利用特定c型细胞色素的信号蛋白的多样性
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-24 DOI: 10.1186/s13213-022-01694-4
D. Ding, Wei Huang, Li-lan Lei, Pu Wu
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引用次数: 0
Impact of Litsea cubeba inter-row cover on the structure of bacterial community in the tea plantation 山苍子行间覆盖对茶园细菌群落结构的影响
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-24 DOI: 10.1186/s13213-022-01696-2
H. Hao, Fei Xia, Hongtong Bai, Xun-you Yan, H. Cui, Xiaomao Xie, Lei Shi
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引用次数: 1
Distribution, cytotoxicity, and antioxidant activity of fungal endophytes isolated from Tsuga chinensis (Franch.) Pritz. in Ha Giang province, Vietnam 从Tsuga chinensis(Franch)Pritz分离的真菌内生菌的分布、细胞毒性和抗氧化活性。在越南河江省
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-19 DOI: 10.1186/s13213-022-01693-5
T. Vu, Ngoc Son Pham, Phuong Le, Q. Pham, N. Quach, V. T. Nguyen, Thi Thao Do, H. H. Chu, Q. Phi
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引用次数: 3
Effects of biochar on soil microbial diversity and community structure in clay soil 生物炭对粘土土壤微生物多样性和群落结构的影响
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-18 DOI: 10.1186/s13213-022-01689-1
Jing Zhang, Jiangzhong Shen
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引用次数: 8
Bacterial and archaeal communities within the alkaline soda Langaco Lake in the Qinghai-Tibet Plateau 青藏高原兰古湖碱性苏打水中细菌和古细菌群落
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-10 DOI: 10.1186/s13213-022-01691-7
M. Wang, Xin Zhang, Zhiwan Shu, Zhibo Wang, Yujie Tao, Chuanbo Lv, Derui Zhu, G. Shen
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引用次数: 0
Insights into the deriving of rhizosphere microenvironments and its effects on the growth of authentic Angelica sinensis seedlings under continuous monoculture 单作条件下根际微环境的形成及其对当归幼苗生长的影响
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-09-10 DOI: 10.1186/s13213-022-01692-6
Xiaofang Gong, Ying Zhu, Yinan Peng, Zengxiang Guo, Jianping Zhou, Hui Yang, Zhiye Wang
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引用次数: 0
Effect of vegetable residues incorporation on soil fertility, rhizosphere microbial community structure, and plant growth of continuously cropped cucumber in a solar greenhouse 蔬菜残茬对日光温室连作黄瓜土壤肥力、根际微生物群落结构和植株生长的影响
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-08-31 DOI: 10.1186/s13213-022-01690-8
Chen, Xiaolu, Zhang, Dalong, Li, Xiaoya, Li, Xiaotian, Lou, Jie, Wei, Min
Incorporating crop residues into the soil is considered a sustainable and valuable method to alleviate soil deterioration caused by continuous monoculture in greenhouse production. However, the effect of vegetable residues retention on soil amendments is poorly understood. In the present study, we investigated the impacts of sweet pepper, tomato, and cucumber plant residues on soil microbial communities and plant growth of continuously cropped cucumber in a solar greenhouse. The 16S rRNA and ITS1 rRNA genes were amplified, and high-throughput sequencing was performed to explore the impacts of vegetable residues incorporation on soil microbial communities. Additionally, soil chemical properties, cucumber root vigor, and fruit yield were measured to assess the impacts of vegetable residues incorporation on continuously cropped soil and cucumber growth. The results showed that incorporating vegetable residues could improve soil buffering capacity, increase the content of soil organic matter and available nutrients, and increased the diversity of soil microorganisms and improved community structure; vegetable residues increased the abundance of beneficial bacteria such as Actinobacteria, Firmicutes, Proteobacteria, and Chloroflexi, while reducing the quantity of soil-borne pathogens such as Bacillariophyta and Acidobacteria. Similar results were observed for the fungal communities: the relative abundance of Ascomycota was decreased to varying degrees, while the relative abundance of Rozellomycota and Basidiomycota was raised. The results demonstrated that vegetable residues incorporation significantly increased cucumber root vigor and enhanced fruit yield. The effects of different types of residues on improving soil properties were ordered sweet pepper plant residues > cucumber plant residues > tomato plant residues, and 20% of sweet pepper plant residues incorporation had the most significant effect on crop yield. In summary, returning vegetable residues alleviated soil continuous cropping obstacles by improving the soil fertility and the diversity and community structure of soil microorganisms, and consequently promoting the growth and yield of greenhouse-grown cucumbers. The findings demonstrated that returning vegetable residues was an effective and sustainable measure for soil amendment during continuous cropping in greenhouse production.
将作物残茬掺入土壤被认为是缓解温室生产中连续单一栽培造成的土壤退化的一种可持续和有价值的方法。然而,蔬菜残留对土壤改良剂的影响尚不清楚。在日光温室中,研究了甜椒、番茄和黄瓜植株残茬对连作黄瓜土壤微生物群落和植株生长的影响。扩增16S rRNA和ITS1 rRNA基因,并进行高通量测序,探讨蔬菜秸秆掺入对土壤微生物群落的影响。此外,通过测定土壤化学性质、黄瓜根系活力和果实产量,评价蔬菜残茬对连作土壤和黄瓜生长的影响。结果表明:蔬菜残茬可提高土壤缓冲能力,提高土壤有机质和速效养分含量,增加土壤微生物多样性,改善群落结构;蔬菜残留物增加了放线菌门、厚壁菌门、变形菌门和绿菌门等有益菌的丰度,同时减少了硅藻门和酸杆菌门等土传病原体的数量。真菌群落也有类似的结果:子囊菌门的相对丰度不同程度地降低,而罗泽菌门和担子菌门的相对丰度则有所提高。结果表明,蔬菜残茬处理能显著提高黄瓜根系活力和果实产量。不同类型残茬对土壤性质的改善效果依次为甜椒残茬>黄瓜残茬>番茄残茬,其中20%的甜椒残茬对作物产量的影响最为显著。综上所述,蔬菜残茬还田通过提高土壤肥力和土壤微生物多样性及群落结构,缓解了土壤连作障碍,从而促进了温室黄瓜的生长和产量。结果表明,在大棚连作过程中,蔬菜残茬还田是一种有效且可持续的土壤改良措施。
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引用次数: 1
Construction of a domestic wastewater disinfection filter from biosynthesized and commercial nanosilver: a comparative study 生物合成纳米银与商用纳米银构筑生活污水消毒滤池的比较研究
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-08-16 DOI: 10.1186/s13213-022-01688-2
Taher, Heba S., Sayed, Rania, Loutfi, Asmaa, Abdulla, Hesham
Biosynthesis of nanoparticles is an eco-friendly process and considered one of the most significant aspects of nanotechnology. Silver nanoparticles (Ag NPs) have a better bactericidal activity due to its high surface area to volume ratio. In this paper, Streptomyces sp. U13 (KP109813) was used to biosynthesize silver nanoparticles (Ag NPs) to construct wastewater disinfection filter. The biosynthesized nanosilver and a commercially available ink nanosilver were characterized, and their wastewater disinfection efficiency was compared. The nanometrological characteristics of both nanosilver such as structure, shape, and size were investigated using the X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FTIR), high-resolution transmission electron microscope (HR-TEM), and UV-visible spectroscopy. The results revealed that the biosynthesized and ink Ag NPs were well dispersed and had a spherical shape, with sizes ranged from 5 to 37 nm and from 2 to 26 nm, respectively. To examine the disinfection capabilities, Ag NPs were loaded on two substrates, foam and limestone gravel, and packed into a glass column receiving domestic wastewater. Results showed that Ag NPs attached to limestone gravel eliminate 100% of the coliform bacteria better than foam. Comparing to control columns (without silver), only 50 and 10% reduction of the total coliform in gravel and foam column were achieved, respectively. This work concluded that the type of substrate controls the amount of Ag NPs loaded on it and thus controls the disinfection process. No significant difference between biosynthesized and ink nanosilver in wastewater disinfection was observed. Using limestone gravel filter loaded with 200 mg/l Ag NPs with contact time of 150 min achieves a complete eradication of coliform bacteria.
纳米粒子的生物合成是一种生态友好的过程,被认为是纳米技术最重要的方面之一。银纳米粒子具有较高的比表面积和体积比,具有较好的杀菌活性。本文以链霉菌U13 (KP109813)为原料,生物合成银纳米粒子(Ag NPs),构建废水消毒滤池。对生物合成的纳米银和市售的油墨纳米银进行了表征,并比较了它们对废水的消毒效果。采用x射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、高分辨率透射电子显微镜(hrtem)和紫外可见光谱对两种纳米银的结构、形状和尺寸等纳米计量学特征进行了研究。结果表明,生物合成的银纳米粒子和油墨银纳米粒子分散良好,呈球形,尺寸分别在5 ~ 37 nm和2 ~ 26 nm之间。为了检验消毒能力,研究人员将银纳米粒子装载在泡沫和石灰石碎石两种基质上,并将其装入一个接收生活废水的玻璃柱中。结果表明,与泡沫相比,附着在石灰石砾石上的Ag NPs对大肠菌群的去除率达到100%。与对照柱(不含银)相比,砾石柱和泡沫柱中大肠菌群总数分别减少了50%和10%。这项工作的结论是,底物的类型控制了负载在其上的银NPs的数量,从而控制了消毒过程。生物合成纳米银与油墨纳米银对废水的消毒效果无显著差异。石灰石砾石过滤器加载200 mg/l Ag NPs,接触时间为150 min,可完全根除大肠菌群。
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引用次数: 1
Application of Weizmannia coagulans in the medical and livestock industry 魏茨曼凝血剂在医疗和畜牧业中的应用
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-08-10 DOI: 10.1186/s13213-022-01687-3
Wang, Linhao, Wang, Jie, Du, Liyu, Fang, Xiang, Liao, Zhenlin
Products enriched with probiotics have always been fashionable. Weizmannia coagulans has become a hot research topic in the academic community due to their multiple functional properties and high resistance to stress, which can retain their activity in a variety of harsh environments. This review aims to evaluate the probiotic effects of different strains of Weizmannia coagulans in animals and humans and to inspire better exploitation of the value of this strain. This review summarizes the latest research progress of Weizmannia coagulans from two major applications in animal breeding and human health. The functional properties of Weizmannia coagulans are extensively recognized. In animals, the strain can promote nutrient absorption, reduce mortality, and enhance the slaughter rate in livestock and poultry. In humans, the strain can be used to treat gastrointestinal disorders, immunomodulation, depressive symptoms, and non-alcoholic fatty liver. Weizmannia coagulans is projected as an ideal substitute for antibiotics and other chemical drugs. Despite the outstanding functional properties of Weizmannia coagulans, there are numerous strains of Weizmannia coagulans and significant differences between strains in functional and physiological properties. Currently, there are few literature reports on the probiotic mechanism and functional gene identification of Weizmannia coagulans, which is crucial for the commercialization of Weizmannia coagulans and the benefit of human society.
富含益生菌的产品一直很流行。Weizmannia混凝剂具有多种功能特性和高抗逆性,在各种恶劣环境下仍能保持活性,成为学术界研究的热点。本文旨在评价不同菌株凝固魏茨曼菌在动物和人体内的益生菌作用,以更好地开发该菌株的价值。本文从凝固剂在动物育种和人体健康方面的应用两方面综述了凝固剂的最新研究进展。Weizmannia凝固剂的功能特性得到了广泛的认可。在动物中,该菌株能促进牲畜和家禽的营养吸收,降低死亡率,提高屠宰率。在人类中,该菌株可用于治疗胃肠道疾病、免疫调节、抑郁症状和非酒精性脂肪肝。魏茨曼凝血剂被认为是抗生素和其他化学药物的理想替代品。尽管凝固魏茨曼菌具有突出的功能特性,但其品系众多,且品系之间在功能和生理特性上存在显著差异。目前,关于魏茨曼凝固剂的益生菌机制和功能基因鉴定的文献报道很少,而这对于魏茨曼凝固剂的商业化和造福人类社会至关重要。
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引用次数: 1
Probiotic Yoghurt Made from Milk of Ewes Fed a Diet Supplemented with Spirulina platensis or Fish Oil 在母羊乳中添加螺旋藻或鱼油制备益生菌酸奶
IF 3 4区 生物学 Q1 Immunology and Microbiology Pub Date : 2022-08-04 DOI: 10.1186/s13213-022-01686-4
Shazly, Ahmed B., Khattab, Mostafa S. A., Fouad, Mohamed T., Abd El Tawab, Ahmed M., Saudi, Eltaher M., El-Aziz, Mahmoud Abd
Yoghurt is a widely consumed dairy product around the world. It has healing properties and characteristics that are important for human health. Our goal was to see how using ewes' milk fed Spirulina platensis (SP) or fish oil (FO)-supplemented diets affected the chemical, physical, and nutritional properties of yoghurt, as well as the activity and survival of starter and probiotic bacteria during storage. The collected milk from each ewe group was preheated to 65 °C and homogenized in a laboratory homogenizer, then heated to 90 °C for 5 min, cooled to 42 °C, and divided into two equal portions. The first portion was inoculated with 2.0% mixed starter culture (Lactobacillus bulgaricus and Streptococcus thermophilus, 1:1), whereas the second was inoculated with 2% mixed starter culture and 1% Bifidobacterium longum as a probiotic bacteria. SP yoghurt had the highest levels of short chain-FA, medium chain-FA, mostly C10:0, and long chain-FA, namely C16:0, C18:2 and the lowest levels of C18:0 and C18:1, followed by FO yoghurt. The addition of SP or FO to ewes' diets resulted in yoghurt with higher viable counts of L. bulgaricus and S. thermophilus, which were still >107 cfu/g at the end of storage, as well as a higher level of acetaldehyde content (P<0.05) as a flavor compound, than the control (C) yoghurt. The viscosity of SP yoghurt was higher than that of FO and C yoghurt; the difference was not significant. The addition of B. longum, a probiotic bacteria, to all yoghurt samples, improved antioxidant activities, particularly against ABTS• radicals, but reduced SP yoghurt viscosity. When B. longum was added, acetaldehyde content increased from 39.91, 90.47, and 129.31 μmol/100g in C, FA, and SP yoghurts to 46.67, 135.55, and 144.1 μmol/100g in probiotic C, FA, and SP yoghurts, respectively. There was no significant difference in sensory qualities among all the yoghurt samples during all storage periods. Supplementing the ewes' diets with Spirulina platensis or fish oil can change the fatty acid composition of the resulting yoghurt. The starter culture's activity, flavor compounds, and some chemical, physical, and antioxidant properties of milk produced from these diets can all be improved, particularly in yoghurt treated with probiotic bacteria (B. longum).
酸奶是世界上广泛消费的乳制品。它具有对人体健康很重要的治疗特性和特性。我们的目的是观察用母奶饲喂螺旋藻(SP)或添加鱼油(FO)的饲料对酸奶的化学、物理和营养特性的影响,以及在储存过程中发酵剂和益生菌的活性和存活率的影响。将各组母羊采集的奶预热至65℃,在实验室均质机中均质,然后加热至90℃5 min,冷却至42℃,分成两等份。第一部分接种2.0%混合发酵剂(保加利亚乳杆菌与嗜热链球菌比例为1:1),第二部分接种2%混合发酵剂和1%长双歧杆菌作为益生菌。SP型酸奶的短链fa含量最高,中链fa含量以c10∶0居多,长链fa含量以c16∶0、c18∶2居多,c18∶0、c18∶1含量最低,FO型酸奶次之。在母羊日粮中添加SP或FO后,发酵乳中保加利亚乳杆菌和嗜热链球菌的活菌数均高于对照(C),贮藏末期仍>107 cfu/g,风味化合物乙醛含量也高于对照(C) (P<0.05)。SP酸奶的粘度高于FO和C酸奶;差异不显著。在所有酸奶样品中添加长芽胞杆菌(一种益生菌),提高了抗氧化活性,特别是抗ABTS•自由基,但降低了SP酸奶的粘度。添加长芽甘蓝后,C、FA和SP酸奶的乙醛含量分别从39.91、90.47和129.31 μmol/100g增加到46.67、135.55和144.1 μmol/100g。不同贮藏期酸奶的感官品质无显著差异。在母羊日粮中添加螺旋藻或鱼油可以改变所得酸奶的脂肪酸组成。发酵剂的活性、风味化合物以及由这些日粮生产的牛奶的一些化学、物理和抗氧化特性都可以得到改善,特别是在用益生菌(长芽孢杆菌)处理的酸奶中。
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引用次数: 2
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Annals of Microbiology
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