{"title":"Use of medium supplements to improve anti-MRSA mAb final concentration in hybridoma cell culture and reduce the cost production","authors":"Rafael Loureiro, J. Senna, Álvaro P. B. Sousa","doi":"10.35259/isi.2022_52156","DOIUrl":"https://doi.org/10.35259/isi.2022_52156","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83625137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amanda Santos, F. Manta, Fabricio K Marchini, M. Moraes, A. Costa
Objective: To optimize and evaluate a qPCR multiplex reaction for the detection of Mycobacterium leprae genomic markers 16S rRNA and RLEP, as well as human 18S rRNA gene as an internal control, using the portable thermocycler Q3-Plus. Methodology: The qPCR multiplex reaction was performed using the commercial kit NAT Hans (IBMP, Brazil) in the portable instrument Q3-Plus (Alifax, Italy). Standard dilution curves of two different samples, a synthetic DNA and DNA extracted from 10 9 M. leprae cells, were used to determine the detection limit and reaction efficiency. Optical parameters such as light intensity, gain, and exposure time were optimized for each target in the Q3-Plus equipment. Results: In the standard equipment, the reactions performed with synthetic positive control showed 94.9% of efficiency to 16S and 93.1% to RLEP, respectively, with a detection limit of 2.29 copies/uL. In Q3-Plus equipment, reactions performed with synthetic positive control were able to amplify 3.67 copies/uL, similar to published results. Reaction efficiency was estimated as 87.12% for the 16S gene target, with a LOD95% of 47.68 copies/uL. For the RLEP target, reactions in the portable instrument presented 86.89% efficiency and a LOD95% of 53.57 copies/uL. Reactions performed with DNA extracted from M. leprae cells showed an efficiency of 95.39% for 16S and 90% for RLEP, with an estimated LOD95% of 1013.18 genome equivalents/uL. Conclusion: Data obtained with the portable instrument show similar efficiency and LOD95% as published results. This represents the first step towards the development of a portable molecular diagnostic test for diagnosis of leprosy in low resource environments.
目的:利用便携式热循环仪Q3-Plus，优化并评价一种qPCR多重反应检测麻风分枝杆菌基因组标记物16S rRNA和RLEP，以及人18S rRNA基因作为内对照。方法:使用商用试剂盒NAT Hans (IBMP，巴西)在便携式仪器Q3-Plus (Alifax，意大利)上进行qPCR多重反应。采用合成DNA和10 9麻风细胞提取DNA两种不同样品的标准稀释曲线，确定检测限和反应效率。对Q3-Plus设备中每个目标的光强、增益和曝光时间等光学参数进行了优化。结果:在标准装置中，合成阳性对照对16S的反应效率为94.9%，对RLEP的反应效率为93.1%，检出限为2.29 copies/uL。在Q3-Plus设备中，用合成阳性对照进行的反应能够扩增3.67个拷贝/uL，与已发表的结果相似。16S基因靶基因的反应效率为87.12%，LOD95%为47.68 copies/uL。对于RLEP目标，便携式仪器的反应效率为86.89%，LOD95%为53.57拷贝/uL。用麻风分枝杆菌细胞提取的DNA进行反应，16S的效率为95.39%，RLEP的效率为90%，估计LOD95%为1013.18个基因组当量/uL。结论:便携式仪器的检测效率与已发表的结果相似，LOD95%。这是朝着开发用于在资源匮乏环境中诊断麻风病的便携式分子诊断测试迈出的第一步。
{"title":"Optimization of a Multiplex qPCR assay in a portable thermocycler for leprosy diagnosis in point of care situations","authors":"Amanda Santos, F. Manta, Fabricio K Marchini, M. Moraes, A. Costa","doi":"10.35259/isi.2022_52177","DOIUrl":"https://doi.org/10.35259/isi.2022_52177","url":null,"abstract":"Objective: To optimize and evaluate a qPCR multiplex reaction for the detection of Mycobacterium leprae genomic markers 16S rRNA and RLEP, as well as human 18S rRNA gene as an internal control, using the portable thermocycler Q3-Plus. Methodology: The qPCR multiplex reaction was performed using the commercial kit NAT Hans (IBMP, Brazil) in the portable instrument Q3-Plus (Alifax, Italy). Standard dilution curves of two different samples, a synthetic DNA and DNA extracted from 10 9 M. leprae cells, were used to determine the detection limit and reaction efficiency. Optical parameters such as light intensity, gain, and exposure time were optimized for each target in the Q3-Plus equipment. Results: In the standard equipment, the reactions performed with synthetic positive control showed 94.9% of efficiency to 16S and 93.1% to RLEP, respectively, with a detection limit of 2.29 copies/uL. In Q3-Plus equipment, reactions performed with synthetic positive control were able to amplify 3.67 copies/uL, similar to published results. Reaction efficiency was estimated as 87.12% for the 16S gene target, with a LOD95% of 47.68 copies/uL. For the RLEP target, reactions in the portable instrument presented 86.89% efficiency and a LOD95% of 53.57 copies/uL. Reactions performed with DNA extracted from M. leprae cells showed an efficiency of 95.39% for 16S and 90% for RLEP, with an estimated LOD95% of 1013.18 genome equivalents/uL. Conclusion: Data obtained with the portable instrument show similar efficiency and LOD95% as published results. This represents the first step towards the development of a portable molecular diagnostic test for diagnosis of leprosy in low resource environments.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79548658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W. Rocha, K. Silva, Milena Pereira, T. Gouvea, C. Kury
{"title":"Impact of vaccination on the circulation of different Human Papillomavirus genotypes in male university students from Rio de Janeiro, Brazil.","authors":"W. Rocha, K. Silva, Milena Pereira, T. Gouvea, C. Kury","doi":"10.35259/isi.2022_52214","DOIUrl":"https://doi.org/10.35259/isi.2022_52214","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86206924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study compared the systemic immune response to IN/intramuscular (IM) and IM/IM delivery of meningococcal outer membrane vesicles (OMVs). Methodology: A/Sn (H2 a ) mice were immunized with 4 subsequent IN doses (0.2 μg OMVs+0.1 μg Cholera toxin subunit B [CTB]) (Sigma-Aldrich) and one IM booster (0.2 μg OMVs+0.2 μg CTB) after 15 days. For comparison, another group received 2 IM doses, 15 days apart (0.2 μg OMVs+0.1 mM Aluminium hidroxyde [AH]). Control groups received only adjuvants. Antigen control received 2 IM doses (0.2 μg OMVs), 15 days apart. The humoral response was assessed by ELISA and serum bactericidal assay (SBA) and the cellular response, by ELISpot. Results: OMVs+CTB had increased IgG titers compared to pre-immune control after the IN doses (p<0.05) and, after booster, it increased even more (p<0.01). OMV+AH was superior to pre-immune (p<0.001) and AH (p<0.05) controls after 2 doses. OMVs alone did not elicit statistically higher titers, although it was higher than pre-immune sera. There was no significance in IgG2a titers, while IgG1 was increased in OMV+CTB and OMV+AH compared to controls (p<0.05 for all). OMVs alone were not bactericidal, while OMV+CTB and OMV+AH were (SBA titers 1/8 and 1/16, respectively). ELISpot was conducted when mice were elderly (after 475) to assess immunologic memory. IL-4 release after antigenic stimuli was higher in OMV+CTB and OMV+AH groups than in OMVs group. The immunization also induced IL-17 release, especially by the OMV+CTB group. Conclusion: Low antigenic doses of OMVs were immunogenic and induced immunologic memory. 4 IN doses were effective to induce systemic IgG. Adjuvants were needed to increase IgG titers and to guarantee bactericidal activity. AH and CTB modulated a Th2 response, with higher IgG1 titers and IL-4 secretion. The IN/IM approach was comparable to the IM/IM one to induce systemic immunity.
{"title":"Comparison of systemic immunity following intranasal/intramuscular and intramuscular immunization with meningococci antigens","authors":"A. Portilho, V. Correa, E. Gaspari","doi":"10.35259/isi.2022_52207","DOIUrl":"https://doi.org/10.35259/isi.2022_52207","url":null,"abstract":"This study compared the systemic immune response to IN/intramuscular (IM) and IM/IM delivery of meningococcal outer membrane vesicles (OMVs). Methodology: A/Sn (H2 a ) mice were immunized with 4 subsequent IN doses (0.2 μg OMVs+0.1 μg Cholera toxin subunit B [CTB]) (Sigma-Aldrich) and one IM booster (0.2 μg OMVs+0.2 μg CTB) after 15 days. For comparison, another group received 2 IM doses, 15 days apart (0.2 μg OMVs+0.1 mM Aluminium hidroxyde [AH]). Control groups received only adjuvants. Antigen control received 2 IM doses (0.2 μg OMVs), 15 days apart. The humoral response was assessed by ELISA and serum bactericidal assay (SBA) and the cellular response, by ELISpot. Results: OMVs+CTB had increased IgG titers compared to pre-immune control after the IN doses (p<0.05) and, after booster, it increased even more (p<0.01). OMV+AH was superior to pre-immune (p<0.001) and AH (p<0.05) controls after 2 doses. OMVs alone did not elicit statistically higher titers, although it was higher than pre-immune sera. There was no significance in IgG2a titers, while IgG1 was increased in OMV+CTB and OMV+AH compared to controls (p<0.05 for all). OMVs alone were not bactericidal, while OMV+CTB and OMV+AH were (SBA titers 1/8 and 1/16, respectively). ELISpot was conducted when mice were elderly (after 475) to assess immunologic memory. IL-4 release after antigenic stimuli was higher in OMV+CTB and OMV+AH groups than in OMVs group. The immunization also induced IL-17 release, especially by the OMV+CTB group. Conclusion: Low antigenic doses of OMVs were immunogenic and induced immunologic memory. 4 IN doses were effective to induce systemic IgG. Adjuvants were needed to increase IgG titers and to guarantee bactericidal activity. AH and CTB modulated a Th2 response, with higher IgG1 titers and IL-4 secretion. The IN/IM approach was comparable to the IM/IM one to induce systemic immunity.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77718647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Teixeira, Renata Pedro, L. Lignani, C. Cordeiro, Patrícia Dalmina de Oliveira
{"title":"Pharmacovigilance Committee for COVID-19 vaccine (ChAdOx1-S [recombinant]) and its contribution for an effective benefit-risk assessment","authors":"G. Teixeira, Renata Pedro, L. Lignani, C. Cordeiro, Patrícia Dalmina de Oliveira","doi":"10.35259/isi.2022_52265","DOIUrl":"https://doi.org/10.35259/isi.2022_52265","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"94 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80297721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kaique Pereira, Vinícius Gonçalves, Melissa Premazzi, P. Jurgilas, R. Bastos
The CPs to be functionalized were obtained via enzymatic hydrolysis and purified by affinity chromatography. The NPs mean diameter and zeta potential (ZP) were determined by Dinamic Light Scattering and Zeta Potential Analyzer. Encapsulation efficiency and Drug Release were made by UV/visible absorption spectrophotometry and reversed phase HPLC (HPLC-RP). PC effect was analyzed by Nano ITC. Microscale Thermophoresis (MST) was employed to verify intermolecular interactions. Results: The NPs average size before functionalization were 224.9 nm with a 0.046 polydispersity index (PDI) and -16.53 mV ZP. After functionalization were 251.3 nm, PDI 0.022, and +0.238 mV ZP, which indicates CP coupling. Intermolecular interaction with a negative charged biomolecule validated functionalization success. Both protocols were efficient to determine encapsulation efficiency by UV/ visible absorption spectrophotometry (46.66%) and by HPLC-RP (46.96%). Data from drug release at 72h showed 64% for free drug against 32% for the encapsulated drug, demonstrating a controlled release. Stability study during 4 weeks provided a 0.067 average PDI; 211.5nm average size and 2% coefficient of variation, which indicates stability of the nanoparticles. The PEGylated NP has showed potential to decrease the PC once it couples its surface at a slower rate than non-PEGylated. Conclusion: The NPs attributes suggest efficiency of functionalization and PEGylation, furthermore showed adequate stability and physicochemical properties as nano-delivery systems.
{"title":"Physicochemical and stability evaluation of functionalized polymeric nanoparticles for potential drug delivery","authors":"Kaique Pereira, Vinícius Gonçalves, Melissa Premazzi, P. Jurgilas, R. Bastos","doi":"10.35259/isi.2022_52293","DOIUrl":"https://doi.org/10.35259/isi.2022_52293","url":null,"abstract":"The CPs to be functionalized were obtained via enzymatic hydrolysis and purified by affinity chromatography. The NPs mean diameter and zeta potential (ZP) were determined by Dinamic Light Scattering and Zeta Potential Analyzer. Encapsulation efficiency and Drug Release were made by UV/visible absorption spectrophotometry and reversed phase HPLC (HPLC-RP). PC effect was analyzed by Nano ITC. Microscale Thermophoresis (MST) was employed to verify intermolecular interactions. Results: The NPs average size before functionalization were 224.9 nm with a 0.046 polydispersity index (PDI) and -16.53 mV ZP. After functionalization were 251.3 nm, PDI 0.022, and +0.238 mV ZP, which indicates CP coupling. Intermolecular interaction with a negative charged biomolecule validated functionalization success. Both protocols were efficient to determine encapsulation efficiency by UV/ visible absorption spectrophotometry (46.66%) and by HPLC-RP (46.96%). Data from drug release at 72h showed 64% for free drug against 32% for the encapsulated drug, demonstrating a controlled release. Stability study during 4 weeks provided a 0.067 average PDI; 211.5nm average size and 2% coefficient of variation, which indicates stability of the nanoparticles. The PEGylated NP has showed potential to decrease the PC once it couples its surface at a slower rate than non-PEGylated. Conclusion: The NPs attributes suggest efficiency of functionalization and PEGylation, furthermore showed adequate stability and physicochemical properties as nano-delivery systems.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80942006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Ferreira, J. Oliveira, Cláudia Ó Pessoa, A. Soares, R. Nicolete
as a form of dynamic monitoring, in real time from start to finish, of phenotypic events of B16F10 tumor cells treated with DOX (0.2 nM) and in association with Cta toxin (200 nM) for more than 72h. Results: The toxin used alone at the minimum concentration of 200 nM exerted 40% toxicity and at higher concentrations (1000 and 5000 nM) decreased cell viability more significantly. Dox, in turn, at concentrations (0.02 – 0.1 nM) showed toxicity between 20 and 80%. On the other hand, and surprisingly, the pharmacological association between Cta (200 nM) and Dox (0.2 nM) was able to exert cytotoxicity around 60%. Conclusion: The combination of the nanocarrier toxin Cta with the chemotherapeutic Doxorubicin in minimal concentrations was able to improve the antiproliferative activity (potentiating effect) observed in B16F10 cells, thus contributing to new studies involving alternative/complementary therapies for the control of cell replication in different tumor lineages and /or in vivo models.
{"title":"Evaluation of the in vitro antitumor effect of the association between doxorubicin and crotamine toxinAssessment of the in vitro antitumor effect of the association between doxorubicin and crotamine toxin","authors":"V. Ferreira, J. Oliveira, Cláudia Ó Pessoa, A. Soares, R. Nicolete","doi":"10.35259/isi.2022_52292","DOIUrl":"https://doi.org/10.35259/isi.2022_52292","url":null,"abstract":"as a form of dynamic monitoring, in real time from start to finish, of phenotypic events of B16F10 tumor cells treated with DOX (0.2 nM) and in association with Cta toxin (200 nM) for more than 72h. Results: The toxin used alone at the minimum concentration of 200 nM exerted 40% toxicity and at higher concentrations (1000 and 5000 nM) decreased cell viability more significantly. Dox, in turn, at concentrations (0.02 – 0.1 nM) showed toxicity between 20 and 80%. On the other hand, and surprisingly, the pharmacological association between Cta (200 nM) and Dox (0.2 nM) was able to exert cytotoxicity around 60%. Conclusion: The combination of the nanocarrier toxin Cta with the chemotherapeutic Doxorubicin in minimal concentrations was able to improve the antiproliferative activity (potentiating effect) observed in B16F10 cells, thus contributing to new studies involving alternative/complementary therapies for the control of cell replication in different tumor lineages and /or in vivo models.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87542239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Santos, Marcelle Mello, Leila Silva, Bernardo Loureiro, C. Vianna
Results: Negative and positive controls, as well as the background, were analyzed in replicates and the 95% of confidence interval was calculated from the arithmetic mean with two errors below and above. The negative control was set to 0.115 (± 0.0407), positive control to 1.007 (±0.125), and the background to 0.058 (±0.008). The robustness of the ELISA was evaluated. Eighteen standard curves of the positive control were analyzed and no statistically significant difference was observed between the Optical Densities (OD) against variations in the incubation temperature (36 - 38 o C) (p=0.0590), conjugated lots (p = 0.2495) and operator (p = 0.9426). The quantification limit was calculated from the analysis of the average of four standard curves of the positive control, with the detection limit from an OD of 0.2 where the analyte produces a signal three times higher than the noise signal (0,06) and quantification limit of 0.6, as long as the signal-to-noise ratio is greater than 6 (9.316 EU/mL). Blood samples from 33 volunteers vaccinated against Covid-19 were analyzed. IgG antibodies concentration were calculated using the 4-logistic parameter. A statistically significant increase in antibody titers (p<0,001) was observed after second dose, and the agreement of the results with the liquid microarray platform will be evaluated. Conclusion: The test should be revalidated if there is a change in the final product. However, our findings suggest that a feasible, useful quantitative ELISA assay was obtained, with the potential of helping to elucidate the antibody response dynamics after Covid-19 natural infection and/or vaccination.
{"title":"Quantitative igg elisa of sars-cov-2 spike-protein: analysis of blood samples from vaccinated individuals","authors":"Ana Santos, Marcelle Mello, Leila Silva, Bernardo Loureiro, C. Vianna","doi":"10.35259/isi.2022_52172","DOIUrl":"https://doi.org/10.35259/isi.2022_52172","url":null,"abstract":"Results: Negative and positive controls, as well as the background, were analyzed in replicates and the 95% of confidence interval was calculated from the arithmetic mean with two errors below and above. The negative control was set to 0.115 (± 0.0407), positive control to 1.007 (±0.125), and the background to 0.058 (±0.008). The robustness of the ELISA was evaluated. Eighteen standard curves of the positive control were analyzed and no statistically significant difference was observed between the Optical Densities (OD) against variations in the incubation temperature (36 - 38 o C) (p=0.0590), conjugated lots (p = 0.2495) and operator (p = 0.9426). The quantification limit was calculated from the analysis of the average of four standard curves of the positive control, with the detection limit from an OD of 0.2 where the analyte produces a signal three times higher than the noise signal (0,06) and quantification limit of 0.6, as long as the signal-to-noise ratio is greater than 6 (9.316 EU/mL). Blood samples from 33 volunteers vaccinated against Covid-19 were analyzed. IgG antibodies concentration were calculated using the 4-logistic parameter. A statistically significant increase in antibody titers (p<0,001) was observed after second dose, and the agreement of the results with the liquid microarray platform will be evaluated. Conclusion: The test should be revalidated if there is a change in the final product. However, our findings suggest that a feasible, useful quantitative ELISA assay was obtained, with the potential of helping to elucidate the antibody response dynamics after Covid-19 natural infection and/or vaccination.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83631986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sucupira, Leila Sucupira, M. Melo, C. Marques, Mariana García
{"title":"New approaches for recombinant protein VP1-2A of HAV production and characterization based on liquid microarray assay: application for developing a point-of care diagnostic test","authors":"M. Sucupira, Leila Sucupira, M. Melo, C. Marques, Mariana García","doi":"10.35259/isi.2022_52153","DOIUrl":"https://doi.org/10.35259/isi.2022_52153","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85473152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Souza, V. Lopes, Gabrielle Barcellos, B. Patrício, Francisco Alexandrino Júnior
nm. The cytotoxicity assay demonstrated that NanoAmB and nanocarriers without AmB does not show cytotoxicity against mammalian cells at concentrations ranging from 10 μg/mL to 0.1 μg/mL. However NanoAmB demonstrated that cytotoxic effect against C. albicans and C. neoformans after 24 hours in all concentration analyzed (10 μg/mL to 0.1 μg/mL) (p<0.05). However, nanocarriers without AmB showed a cytotoxic effect from 10 to 2.5 μg/Ml. NanoAmB showed an effect from 1.25 to 0.05 μg/mL in both fungal species and a partial effect at concentration up to 0.025 μg/mL only in C. albicans (p<0.05). Concentrations lower than 0.025 showed no cytotoxic effect. Conclusion: Nanocarriers acted as an enhancing agent, potentializing the inhibitory growth effects of AmB on pathogenic fungi. Other novel antifungal therapeutic strategies using NanoAmB, isolated or in combination with mAbs, should be considered in the future.
{"title":"Evaluation effect of nanocarriers of AmB on pathogenics fungal","authors":"C. Souza, V. Lopes, Gabrielle Barcellos, B. Patrício, Francisco Alexandrino Júnior","doi":"10.35259/isi.2022_52291","DOIUrl":"https://doi.org/10.35259/isi.2022_52291","url":null,"abstract":"nm. The cytotoxicity assay demonstrated that NanoAmB and nanocarriers without AmB does not show cytotoxicity against mammalian cells at concentrations ranging from 10 μg/mL to 0.1 μg/mL. However NanoAmB demonstrated that cytotoxic effect against C. albicans and C. neoformans after 24 hours in all concentration analyzed (10 μg/mL to 0.1 μg/mL) (p<0.05). However, nanocarriers without AmB showed a cytotoxic effect from 10 to 2.5 μg/Ml. NanoAmB showed an effect from 1.25 to 0.05 μg/mL in both fungal species and a partial effect at concentration up to 0.025 μg/mL only in C. albicans (p<0.05). Concentrations lower than 0.025 showed no cytotoxic effect. Conclusion: Nanocarriers acted as an enhancing agent, potentializing the inhibitory growth effects of AmB on pathogenic fungi. Other novel antifungal therapeutic strategies using NanoAmB, isolated or in combination with mAbs, should be considered in the future.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73047976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：