G. Sartori, E. Gaieta, Victoria Moreira, Samuel Almeida, J. Sampaio
in literature. Finally, the modified antibody does not recognize the original antigen, since while the original antibody had its correct pose readily identified, by docking, the mutated antibody not even generated the crystallographic pose. Conclusion: Here we described and applied a new protocol for computationally design new antibodies taking advantage of the structural variability of CDRs loops at low computational cost.
{"title":"Development of computational pipeline for antibody identification against specific conformations of PD-1.","authors":"G. Sartori, E. Gaieta, Victoria Moreira, Samuel Almeida, J. Sampaio","doi":"10.35259/isi.2022_52283","DOIUrl":"https://doi.org/10.35259/isi.2022_52283","url":null,"abstract":"in literature. Finally, the modified antibody does not recognize the original antigen, since while the original antibody had its correct pose readily identified, by docking, the mutated antibody not even generated the crystallographic pose. Conclusion: Here we described and applied a new protocol for computationally design new antibodies taking advantage of the structural variability of CDRs loops at low computational cost.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"3230 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86591146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ana Ajorio, Michel G Chagas, V. Rhodes, A. Rodrigues, E. Fonseca
3)°C for 97 days, and at (22.5± 2.5)°C for 3 days. Conclusion: It was concluded that the RVC batch can be used as a RM in routine analysis for potency and identity assays when stored at (-50 ± 30)°C and in aliquots stored at (5 ± 3)°C for 97 days, since its established properties were stable during this time period.
{"title":"Establishment of a reference material for potency and identity assays of recombinant COVID-19 vaccine active ingredients, intermediary and final products","authors":"Ana Ajorio, Michel G Chagas, V. Rhodes, A. Rodrigues, E. Fonseca","doi":"10.35259/isi.2022_52267","DOIUrl":"https://doi.org/10.35259/isi.2022_52267","url":null,"abstract":"3)°C for 97 days, and at (22.5± 2.5)°C for 3 days. Conclusion: It was concluded that the RVC batch can be used as a RM in routine analysis for potency and identity assays when stored at (-50 ± 30)°C and in aliquots stored at (5 ± 3)°C for 97 days, since its established properties were stable during this time period.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88580799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bárbara B. Mendes, Severino Mendes, R. Mendes, Suelen Lima, I. Peña, L. Pena
{"title":"Development and Validation of Reverse Transcription Loop- Mediated Isothermal Amplification (RT-LAMP) for Rapid Detection of SARS-CoV-2 in Human Samples","authors":"Bárbara B. Mendes, Severino Mendes, R. Mendes, Suelen Lima, I. Peña, L. Pena","doi":"10.35259/isi.2022_52152","DOIUrl":"https://doi.org/10.35259/isi.2022_52152","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85264387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Oliveira, L. Couto, Nathalia Pinho, P. Cuervo, A. Cruz
{"title":"Identification of immunodominant proteins from the Viannia and Leishmania subgenera for the composition of a pan vaccine specific for leishmaniasis","authors":"P. Oliveira, L. Couto, Nathalia Pinho, P. Cuervo, A. Cruz","doi":"10.35259/isi.2022_52197","DOIUrl":"https://doi.org/10.35259/isi.2022_52197","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76611144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Second-generation Vaccines: Adjuvants and Booster Doses","authors":"G. Lima, A. Portilho, E. Gaspari","doi":"10.35259/isi.2022_52154","DOIUrl":"https://doi.org/10.35259/isi.2022_52154","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76694249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rebeca Miranda, G. Monteiro, Luiza Vasconcellos, Samara Silva, L. Costa
to alcohol 70% (0/36) was not able to reduce the biofilm formed ( p = 1.00). Quaternary ammonium (2/36, 5.5%) ( p ≤ 0.0023), sodium hypochlorite at 0.1% and 0.5% were able to reduce the biofilm in 38.8% and 94.4% ( p ≤ 1.32x10 -6 ), respectively. In concentrations ≥ 1.0%, sodium hypochloride eliminated 100% of biofilms. Conclusion: In conclusion, sodium hypochlorite at concentrations ≥ 1.0 %/15 min seems to be the most effective disinfectant for S. maltophilia biofilm elimination. As sodium hypochlorite cannot be applied in certain surfaces due to its corrosive action, other studies are necessary in order to find alternative disinfectants.
{"title":"Evaluation of stenotrophomonas maltophilia biofilm tolerance to disinfectants used in a pharmaceutical industry","authors":"Rebeca Miranda, G. Monteiro, Luiza Vasconcellos, Samara Silva, L. Costa","doi":"10.35259/isi.2022_52295","DOIUrl":"https://doi.org/10.35259/isi.2022_52295","url":null,"abstract":"to alcohol 70% (0/36) was not able to reduce the biofilm formed ( p = 1.00). Quaternary ammonium (2/36, 5.5%) ( p ≤ 0.0023), sodium hypochlorite at 0.1% and 0.5% were able to reduce the biofilm in 38.8% and 94.4% ( p ≤ 1.32x10 -6 ), respectively. In concentrations ≥ 1.0%, sodium hypochloride eliminated 100% of biofilms. Conclusion: In conclusion, sodium hypochlorite at concentrations ≥ 1.0 %/15 min seems to be the most effective disinfectant for S. maltophilia biofilm elimination. As sodium hypochlorite cannot be applied in certain surfaces due to its corrosive action, other studies are necessary in order to find alternative disinfectants.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72739668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Mirotti, M. Russo, M. Correa, Mario Hirata, Felipe Silva
{"title":"Nasal COVID-19 vaccine","authors":"L. Mirotti, M. Russo, M. Correa, Mario Hirata, Felipe Silva","doi":"10.35259/isi.2022_52260","DOIUrl":"https://doi.org/10.35259/isi.2022_52260","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80187187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Souza, P. Azevedo, Lídia P. Faustino, M. Fumagalli, M. Figueiredo
{"title":"Evaluation of the immune response and protection induced by a DNA-based vaccine against SARS-CoV-2","authors":"N. Souza, P. Azevedo, Lídia P. Faustino, M. Fumagalli, M. Figueiredo","doi":"10.35259/isi.2022_52210","DOIUrl":"https://doi.org/10.35259/isi.2022_52210","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"84 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88916320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fernanda Maia, Laura Ataídes, F. Conte, Rodrigo Silva
and virulence-associated proteins. These proteins presented two (LIC13321-E1; LIC13321-E2) and four (LIC10715-E1, LIC10715-E2, LIC10715-E3, LIC10715-E4) predicted B-cell linear epitopes. Regarding the natural immunogenicity of predicted epitopes, about 8% and 25% of LG presented antibodies against LIC13321-E1 and LIC13321-E2, respectively. Moreover, while only 6% of LG presented antibodies against LIC10715-E2, other LIC10715 epitopes were specifically recognized by more than 25% of studied individuals. Conclusion: Four naturally immunogenic B-cell epitopes were identified in Leptospira thermolysins and will be evaluated about their protective potential to further compose novel multi-epitopes vaccines.
{"title":"Identification of naturally immunogenic B-cell epitopes in Leptospira secreted metalloproteases: novel and promising targets to multi-epitopes vaccines against leptospirosis","authors":"Fernanda Maia, Laura Ataídes, F. Conte, Rodrigo Silva","doi":"10.35259/isi.2022_52271","DOIUrl":"https://doi.org/10.35259/isi.2022_52271","url":null,"abstract":"and virulence-associated proteins. These proteins presented two (LIC13321-E1; LIC13321-E2) and four (LIC10715-E1, LIC10715-E2, LIC10715-E3, LIC10715-E4) predicted B-cell linear epitopes. Regarding the natural immunogenicity of predicted epitopes, about 8% and 25% of LG presented antibodies against LIC13321-E1 and LIC13321-E2, respectively. Moreover, while only 6% of LG presented antibodies against LIC10715-E2, other LIC10715 epitopes were specifically recognized by more than 25% of studied individuals. Conclusion: Four naturally immunogenic B-cell epitopes were identified in Leptospira thermolysins and will be evaluated about their protective potential to further compose novel multi-epitopes vaccines.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82678087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Soares, B. Santos, Jouber Rocha, Izabella Santos, Y. Mendes
Objective: The aim of this study was to evaluate the heat inactivation of the Attenuated Yellow Fever vaccine residue contained in the bottle and accessories (hoses/needles of the filling system) using hot WFI water (90 o C) available at the DEPFI/Bio-Manguinhos/FIOCRUZ filling area. Methodology: After the filling process, approximately 20 or 45 liters of WFI water ≥80°C were added to the container with the vaccine residue. This volume of hot water was rinsed, including the filling system (hoses and pumps), and 50 ml samples were collected at the end of the line, at each established time: initial t0’, t05 ́, t10’, t15 ́, t20’ and t30’ and the temperature monitored during these time intervals. Three assays were performed to evaluate the inactivation by plaque assay using Vero cell culture. Results: The first viral inactivation assay was performed with approximately 20 L of hot water. The filling bottle temperature varied from 67.5 to 63.5 o C during the 20 minutes of the experiment. The results showed a reduction of the viral titer of 97.66% in the first 5 minutes and of 99.88% in 20 minutes. For the following tests, the volume of hot water added to the filling bottle was changed to 45 L, which increased the inactivation temperature in the filling bottle to the range of 66 to 71 o C. Under these conditions, it was possible to observe a 100% reduction in the viral titer after 30 minutes (2nd assay) and confirmation of these results is in progress (3rd assay). Conclusion: The initial results indicate the possibility that the proposed strategy of heat inactivation for the Attenuated Yellow Fever vaccine residue contained in the flask and accessories of the filling system using hot water could potentially applicable in the DEPFI/Bio-Manguinhos area and could be evaluated for other decontaminations in the unit. Further experiments will be needed to confirm these results.
{"title":"Evaluation of heat inactivation of yellow fever vaccine residue in the filling bottle","authors":"R. Soares, B. Santos, Jouber Rocha, Izabella Santos, Y. Mendes","doi":"10.35259/isi.2022_52272","DOIUrl":"https://doi.org/10.35259/isi.2022_52272","url":null,"abstract":"Objective: The aim of this study was to evaluate the heat inactivation of the Attenuated Yellow Fever vaccine residue contained in the bottle and accessories (hoses/needles of the filling system) using hot WFI water (90 o C) available at the DEPFI/Bio-Manguinhos/FIOCRUZ filling area. Methodology: After the filling process, approximately 20 or 45 liters of WFI water ≥80°C were added to the container with the vaccine residue. This volume of hot water was rinsed, including the filling system (hoses and pumps), and 50 ml samples were collected at the end of the line, at each established time: initial t0’, t05 ́, t10’, t15 ́, t20’ and t30’ and the temperature monitored during these time intervals. Three assays were performed to evaluate the inactivation by plaque assay using Vero cell culture. Results: The first viral inactivation assay was performed with approximately 20 L of hot water. The filling bottle temperature varied from 67.5 to 63.5 o C during the 20 minutes of the experiment. The results showed a reduction of the viral titer of 97.66% in the first 5 minutes and of 99.88% in 20 minutes. For the following tests, the volume of hot water added to the filling bottle was changed to 45 L, which increased the inactivation temperature in the filling bottle to the range of 66 to 71 o C. Under these conditions, it was possible to observe a 100% reduction in the viral titer after 30 minutes (2nd assay) and confirmation of these results is in progress (3rd assay). Conclusion: The initial results indicate the possibility that the proposed strategy of heat inactivation for the Attenuated Yellow Fever vaccine residue contained in the flask and accessories of the filling system using hot water could potentially applicable in the DEPFI/Bio-Manguinhos area and could be evaluated for other decontaminations in the unit. Further experiments will be needed to confirm these results.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90730271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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