the established approval limits. Conclusion: The results demonstrate that only the 5-dose presentation, with a 67% reduction in filling volume, replacement of the current vial model and recalculation of vaccine component concentrations, maintains the productive capacity with the number of doses per batch produced, in the same time and lyophilization cycle currently used.
{"title":"Establishment 5 doses presentation of the triple viral vaccine without albumin to reduce loss during administration","authors":"S. Assumpção, M. Freire, E. Caride, C. Crespo","doi":"10.35259/isi.2022_52264","DOIUrl":"https://doi.org/10.35259/isi.2022_52264","url":null,"abstract":"the established approval limits. Conclusion: The results demonstrate that only the 5-dose presentation, with a 67% reduction in filling volume, replacement of the current vial model and recalculation of vaccine component concentrations, maintains the productive capacity with the number of doses per batch produced, in the same time and lyophilization cycle currently used.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73327851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Generation and characterization of anti-CD19 CAR-T cells overexpressing the protein PHF19","authors":"K. Hajdu, Leonardo Silva, M. Bonamino","doi":"10.35259/isi.2022_52280","DOIUrl":"https://doi.org/10.35259/isi.2022_52280","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74592438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
of T cell activation molecules IFN-g production in response to QTF We immunophenotyped the remaining blood cells used in the QTF test. Our preliminary results show that, as expected, most of the cells present in the lymphocyte gate are CD45+/CD3+ with a median of 77.8% (Tube N, Nill); 82.5% (Mitogen, M); 74.03% (TB1) and 76.75% (TB2). Considering CD4+ T cells, we detected 56% (N), 44% (M), 56% (TB1) and 55% (TB2). On the other hand, we showed that there were 28% (N), 25% (M), 31% (TB1) and 29% (TB2) of CD8+ T cells in the tubes. When we looked for activation markers (CD69, CD71 and HLA-DR) we showed that when T cells are stimulated with mitogen there are higher percentages of T cells, TCD4+ TCD8+ expressing these markers. For the antigen-specific tubes (TB1 and TB2) there were not enough QTF positive samples to conclude the correlation of IFN-g production with the expression of these activation markers. Conclusion: We conclude that it is possible to better characterize the immune phenotype of remaining blood from IGRA.
{"title":"Optimizing usage of remaining blood from Interferon Gamma Releasing Assay (IGRA) to search for immune signatures and biomarkers for Latent Tuberculosis Infection (LTBI)","authors":"B. Ferreira, M. Nunes, D. Rodrigues, P. Rigato","doi":"10.35259/isi.2022_52179","DOIUrl":"https://doi.org/10.35259/isi.2022_52179","url":null,"abstract":"of T cell activation molecules IFN-g production in response to QTF We immunophenotyped the remaining blood cells used in the QTF test. Our preliminary results show that, as expected, most of the cells present in the lymphocyte gate are CD45+/CD3+ with a median of 77.8% (Tube N, Nill); 82.5% (Mitogen, M); 74.03% (TB1) and 76.75% (TB2). Considering CD4+ T cells, we detected 56% (N), 44% (M), 56% (TB1) and 55% (TB2). On the other hand, we showed that there were 28% (N), 25% (M), 31% (TB1) and 29% (TB2) of CD8+ T cells in the tubes. When we looked for activation markers (CD69, CD71 and HLA-DR) we showed that when T cells are stimulated with mitogen there are higher percentages of T cells, TCD4+ TCD8+ expressing these markers. For the antigen-specific tubes (TB1 and TB2) there were not enough QTF positive samples to conclude the correlation of IFN-g production with the expression of these activation markers. Conclusion: We conclude that it is possible to better characterize the immune phenotype of remaining blood from IGRA.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83297598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paulo Silva, Pedro Correia, Daiane Grugel, Victor Ferreira, Rayane Gonçalves
period of less than 1 year of production, as 391 batches were produced. All variables evaluated are under control or on alert. As this is a new product, it is understood that some variations may be inherent to the process and therefore, all will continue to be evaluated on a quarterly basis until completing the period of one more year for the evaluation of phase 3b of the CPV.
{"title":"Continued Process Verification for COVID-19 Vaccine Formulation and Packaging (recombinant)","authors":"Paulo Silva, Pedro Correia, Daiane Grugel, Victor Ferreira, Rayane Gonçalves","doi":"10.35259/isi.2022_52269","DOIUrl":"https://doi.org/10.35259/isi.2022_52269","url":null,"abstract":"period of less than 1 year of production, as 391 batches were produced. All variables evaluated are under control or on alert. As this is a new product, it is understood that some variations may be inherent to the process and therefore, all will continue to be evaluated on a quarterly basis until completing the period of one more year for the evaluation of phase 3b of the CPV.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81774036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Technological roadmap in covid-19 vaccine production","authors":"Silvia Santos, Wanise Barroso","doi":"10.35259/isi.2022_52278","DOIUrl":"https://doi.org/10.35259/isi.2022_52278","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82330482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carla Almeida, M. Stávale, Fernanda Santos, Patricia Baptista
{"title":"Scale up of molecular kit for diagnostic of COVID-19 during pandemic period","authors":"Carla Almeida, M. Stávale, Fernanda Santos, Patricia Baptista","doi":"10.35259/isi.2022_52178","DOIUrl":"https://doi.org/10.35259/isi.2022_52178","url":null,"abstract":"","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85813280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Vitral, Samuel Machado, Camila Santos, E. Pinto, Leonardo Abreu
measles, varicella meningococcal 63.8% indicated by SUS. Conclusion: The study showed an overall low vaccination coverage among health science students along with a poor perception about vaccination schedules. These results are worrisome, considering that these future HCWs will guide the population in the use of vaccines. To overcome this, the study of vaccines and related diseases should be envisaged and deepened as part of the health science courses curricula.
{"title":"A significant portion of undergraduate health science students are not immunized as they should","authors":"C. Vitral, Samuel Machado, Camila Santos, E. Pinto, Leonardo Abreu","doi":"10.35259/isi.2022_52217","DOIUrl":"https://doi.org/10.35259/isi.2022_52217","url":null,"abstract":"measles, varicella meningococcal 63.8% indicated by SUS. Conclusion: The study showed an overall low vaccination coverage among health science students along with a poor perception about vaccination schedules. These results are worrisome, considering that these future HCWs will guide the population in the use of vaccines. To overcome this, the study of vaccines and related diseases should be envisaged and deepened as part of the health science courses curricula.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89644748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Darleide Correia, Esdras Gueiros, Wagner J T Santos, A. Rocha, L. Alves
and specificity of 73% were determined when compared to SNAP 4Dx. Conclusion: The findings revealed that the W. bancrofti antigen is not ideal for the immunodiagnosis of canine heartworm disease using the indirect ELISA assay. However, it was the first Brazilian ELISA developed to search for antibodies in dogs with heartworm disease. Also, the comparison with the SNAP 4Dx, an antigen-capture assay, is not ideal. Some infected animals do not produce antibodies and false negatives in SNAP4Dx could be infected and produce antibodies. It is possibly the reason for the greater positivity seen for the negative group from the endemic area in comparison with those from a non-endemic locality. Further studies aiming at the development of antibody tests for heartworm disease should be pursued, so as to better define the real status of the canine immune response in endemic and non-endemic areas.
{"title":"Evaluation of a Wuchereria bancrofti recombinant antigen for the capture antibody diagnosis of dog heartworm","authors":"Darleide Correia, Esdras Gueiros, Wagner J T Santos, A. Rocha, L. Alves","doi":"10.35259/isi.2022_52182","DOIUrl":"https://doi.org/10.35259/isi.2022_52182","url":null,"abstract":"and specificity of 73% were determined when compared to SNAP 4Dx. Conclusion: The findings revealed that the W. bancrofti antigen is not ideal for the immunodiagnosis of canine heartworm disease using the indirect ELISA assay. However, it was the first Brazilian ELISA developed to search for antibodies in dogs with heartworm disease. Also, the comparison with the SNAP 4Dx, an antigen-capture assay, is not ideal. Some infected animals do not produce antibodies and false negatives in SNAP4Dx could be infected and produce antibodies. It is possibly the reason for the greater positivity seen for the negative group from the endemic area in comparison with those from a non-endemic locality. Further studies aiming at the development of antibody tests for heartworm disease should be pursued, so as to better define the real status of the canine immune response in endemic and non-endemic areas.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81585079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
gene expression regulators. expression on this to target the elements that interact with Sp1 such as histone deacetylase 1 (HDAC1). The interaction of its domain and the C-terminal region of Sp1 inhibits the connection of HDAC1 with the promoter region of genes and repress gene expression. Objective: Considering the role of post-translational modifications in determining the transcriptional activity of Sp1 and the interaction with other proteins such as HDAC1, we report a system for the development of inhibitors of the HDAC1/Sp1 complex using mammalian two-hybrid system. Methodology: In this strategic approach, the DNA-binding domain and the transcriptional activation domain are produced by separate plasmids and become closely associated when the protein HDAC1 fused to a DNA-binding domain, interacts with the protein Sp1 fused to a transcriptional activation domain. The interaction between the proteins results in transcription of the firefly luciferase reporter gene. Results: With this experimental system we can select substances that inhibit the HDAC1/Sp1 interaction and use them in the development of anticancer drugs based on the activation of tumor suppressor genes regulated by Sp1/HDAC1 complex. Conclusion: Our system is applicable to the screening of HDAC1/Sp1 binding inhibitors to assess their antitumor and toxicity activity, but due to the complexity of histone modifications and transcriptional initiation, we cannot rule out the involvement of other epigenetic enzymes or transcription factors.
{"title":"Screening system development for HDAC1/Sp1 complex inhibitors","authors":"F. Verza, Ana Saltoratto, M. Marins","doi":"10.35259/isi.2022_52294","DOIUrl":"https://doi.org/10.35259/isi.2022_52294","url":null,"abstract":"gene expression regulators. expression on this to target the elements that interact with Sp1 such as histone deacetylase 1 (HDAC1). The interaction of its domain and the C-terminal region of Sp1 inhibits the connection of HDAC1 with the promoter region of genes and repress gene expression. Objective: Considering the role of post-translational modifications in determining the transcriptional activity of Sp1 and the interaction with other proteins such as HDAC1, we report a system for the development of inhibitors of the HDAC1/Sp1 complex using mammalian two-hybrid system. Methodology: In this strategic approach, the DNA-binding domain and the transcriptional activation domain are produced by separate plasmids and become closely associated when the protein HDAC1 fused to a DNA-binding domain, interacts with the protein Sp1 fused to a transcriptional activation domain. The interaction between the proteins results in transcription of the firefly luciferase reporter gene. Results: With this experimental system we can select substances that inhibit the HDAC1/Sp1 interaction and use them in the development of anticancer drugs based on the activation of tumor suppressor genes regulated by Sp1/HDAC1 complex. Conclusion: Our system is applicable to the screening of HDAC1/Sp1 binding inhibitors to assess their antitumor and toxicity activity, but due to the complexity of histone modifications and transcriptional initiation, we cannot rule out the involvement of other epigenetic enzymes or transcription factors.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89190497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laura Ataídes, Fernanda Maia, F. Conte, Rodrigo Silva
1, a similar pattern of peptide 2, where 5% of the non-reactive samples (4) presented antibodies against this peptide. Conclusion: Through immunoinformatics it was possible to identify 2 epitopes in this protein. The ELISA tests allowed the evaluation of the immunogenicity of peptides 1 and 2 against antibodies present in the serum of patients. We believe that this study can contribute to advances in vaccine research against leptospirosis.
{"title":"In silico identification of epitopes target of humoral response against Sphingomyelinase 2 (Sph2) of pathogenic Leptospira","authors":"Laura Ataídes, Fernanda Maia, F. Conte, Rodrigo Silva","doi":"10.35259/isi.2022_52218","DOIUrl":"https://doi.org/10.35259/isi.2022_52218","url":null,"abstract":"1, a similar pattern of peptide 2, where 5% of the non-reactive samples (4) presented antibodies against this peptide. Conclusion: Through immunoinformatics it was possible to identify 2 epitopes in this protein. The ELISA tests allowed the evaluation of the immunogenicity of peptides 1 and 2 against antibodies present in the serum of patients. We believe that this study can contribute to advances in vaccine research against leptospirosis.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91453765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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