Meriam Hazgui, M. Weslati, Donia Ounissi, Rahma Boughriba, D. Bacha, Basma Loueslati
The balance between pro- and anti-inflammatory cytokine expression is essential for an efficient immune response and for the regulation of cancer development and progression. This study analyzed the expression and genetic variation in IL-1?, IL-6 and IL-10 genes and the possible associations with colorectal cancer (CRC) and colorectal liver metastases (CRLM).We examined IL-1?, IL-6 and IL-10 mRNA expression and three gene variants: IL-1? (rs1143627), IL-10 (rs1800872) and IL-6 (rs1800795), in 198 CRC, 65 CRLM patients and 230 controls. Carriers of the C/T genotype of IL-1? (rs1143627) have an increased risk of developing CRC and CRLM. T/T genotype carriers have a higher risk of CRLM incidence. For IL-10 (rs1800872), patients harboring the C/A genotype have a lower risk of CRC and CRLM occurrence. For IL-6 (rs1800795), the C/C genotype heightens the risk of CRLM development. Overall survival analysis showed that carriers of the C/T genotype of IL-1? (rs1143627) have a worse overall survival in CRC patients. It can be concluded that interleukin genetic variants can be used as biomarkers to detect and predict clinical outcomes and prognostic factors for CRC and CRLM.
{"title":"Interleukin-1β, interleukin-6 and interleukin-10 polymorphisms in Tnisian patients with colorectal cancer and liver metastasis","authors":"Meriam Hazgui, M. Weslati, Donia Ounissi, Rahma Boughriba, D. Bacha, Basma Loueslati","doi":"10.2298/abs220607032h","DOIUrl":"https://doi.org/10.2298/abs220607032h","url":null,"abstract":"The balance between pro- and anti-inflammatory cytokine expression is essential for an efficient immune response and for the regulation of cancer development and progression. This study analyzed the expression and genetic variation in IL-1?, IL-6 and IL-10 genes and the possible associations with colorectal cancer (CRC) and colorectal liver metastases (CRLM).We examined IL-1?, IL-6 and IL-10 mRNA expression and three gene variants: IL-1? (rs1143627), IL-10 (rs1800872) and IL-6 (rs1800795), in 198 CRC, 65 CRLM patients and 230 controls. Carriers of the C/T genotype of IL-1? (rs1143627) have an increased risk of developing CRC and CRLM. T/T genotype carriers have a higher risk of CRLM incidence. For IL-10 (rs1800872), patients harboring the C/A genotype have a lower risk of CRC and CRLM occurrence. For IL-6 (rs1800795), the C/C genotype heightens the risk of CRLM development. Overall survival analysis showed that carriers of the C/T genotype of IL-1? (rs1143627) have a worse overall survival in CRC patients. It can be concluded that interleukin genetic variants can be used as biomarkers to detect and predict clinical outcomes and prognostic factors for CRC and CRLM.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mushrooms are widely used in many cultures for nutritional and health benefits. The Lactarius species is found in the Aegean region of Turkey. Lactarius chrysorrheus Fr. is a wild mushroom that contains a milky juice. In this study, we investigated the in vitro cytotoxic potential and apoptotic effect of the polysaccharide extract prepared from L. chrysorrheus by water extraction and alcohol precipitation using the tetrazolium MTT dye assay, annexin V staining, wound healing and colony formation, and qRT-PCR. The molecular weights of three peaks observed in HPLC chromatograms were calculated as 1869.9, 3043.92 and 16821.47 Da. The extract exhibited cytotoxic activity at 72 h, with an IC50 value of 296.42 ?g/mL in HepG2 and 444.43 ?g/mL in PANC-1 cells; the extract that was tested on the normal HEK293 cell line exhibited no cytotoxicity. Further, L. chrysorrheus upregulated the expression of CASPASE 3 and CASPASE 9 while downregulating B-cell lymphoma 2 (BCL-2) and B-cell lymphoma-extra large (Bcl-xL) genes, and inhibited cell migration and colony formation in HepG2 and PANC-1 cells. This study provides new insight into the use of the polysaccharide from L. chrysorrheus in the development of novel anticancer agents.
{"title":"Anticancer and apoptotic effects of a polysaccharide extract isolated from Lactarius chrysorrheus Fr. in HepG2 and PANC-1 cell lines","authors":"Dogukan Mutlu, C. Cakir, M. Ozturk, Ş. Arslan","doi":"10.2298/abs220803030m","DOIUrl":"https://doi.org/10.2298/abs220803030m","url":null,"abstract":"Mushrooms are widely used in many cultures for nutritional and health benefits. The Lactarius species is found in the Aegean region of Turkey. Lactarius chrysorrheus Fr. is a wild mushroom that contains a milky juice. In this study, we investigated the in vitro cytotoxic potential and apoptotic effect of the polysaccharide extract prepared from L. chrysorrheus by water extraction and alcohol precipitation using the tetrazolium MTT dye assay, annexin V staining, wound healing and colony formation, and qRT-PCR. The molecular weights of three peaks observed in HPLC chromatograms were calculated as 1869.9, 3043.92 and 16821.47 Da. The extract exhibited cytotoxic activity at 72 h, with an IC50 value of 296.42 ?g/mL in HepG2 and 444.43 ?g/mL in PANC-1 cells; the extract that was tested on the normal HEK293 cell line exhibited no cytotoxicity. Further, L. chrysorrheus upregulated the expression of CASPASE 3 and CASPASE 9 while downregulating B-cell lymphoma 2 (BCL-2) and B-cell lymphoma-extra large (Bcl-xL) genes, and inhibited cell migration and colony formation in HepG2 and PANC-1 cells. This study provides new insight into the use of the polysaccharide from L. chrysorrheus in the development of novel anticancer agents.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glioblastoma is an aggressive, common and deadly primary intracranial brain tumor in adults. The antitumor activity of erianin, a dibenzyl compound found in Dendrobium chrysotoxum Lindl. extract, has not been previously demonstrated in glioblastoma. We investigated the anticancer activity and underlying mechanisms of erianin in human U373 and A172 glioma cells. The effects of erianin on cell viability, apoptosis, migration and invasion were estimated by the XTT test, the reverse transcription-polymerase chain reaction (RT-PCR)/annexin V wound healing assay, and Matrigel? invasion chamber, respectively. The effective amounts of erianin in U373 and A172 cells were 16 and 64 ?M at 48 h, respectively. Erianin also significantly induced apoptosis by inhibiting B-cell lymphoma 2 (Bcl-2), caspase-8, caspase-9 and tumor necrosis factor receptor type 1-associated DEATH domain protein (TRADD), and activation of caspase-3 and BH3 interacting domain death agonist (BID) gene expression. In addition, erianin significantly increased the number of apoptotic cells in U373 and A172 cells and significantly decreased invasion and migration in U373 and A172 cells. Taken together, our results suggest that erianin may be a new therapeutic anticancer drug component with a potent apoptotic effect and a potential for treating glioblastoma.
{"title":"Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells","authors":"Kocoglu Serter, Mücahit Seçme, L. Elmas","doi":"10.2298/abs220219021s","DOIUrl":"https://doi.org/10.2298/abs220219021s","url":null,"abstract":"Glioblastoma is an aggressive, common and deadly primary intracranial brain tumor in adults. The antitumor activity of erianin, a dibenzyl compound found in Dendrobium chrysotoxum Lindl. extract, has not been previously demonstrated in glioblastoma. We investigated the anticancer activity and underlying mechanisms of erianin in human U373 and A172 glioma cells. The effects of erianin on cell viability, apoptosis, migration and invasion were estimated by the XTT test, the reverse transcription-polymerase chain reaction (RT-PCR)/annexin V wound healing assay, and Matrigel? invasion chamber, respectively. The effective amounts of erianin in U373 and A172 cells were 16 and 64 ?M at 48 h, respectively. Erianin also significantly induced apoptosis by inhibiting B-cell lymphoma 2 (Bcl-2), caspase-8, caspase-9 and tumor necrosis factor receptor type 1-associated DEATH domain protein (TRADD), and activation of caspase-3 and BH3 interacting domain death agonist (BID) gene expression. In addition, erianin significantly increased the number of apoptotic cells in U373 and A172 cells and significantly decreased invasion and migration in U373 and A172 cells. Taken together, our results suggest that erianin may be a new therapeutic anticancer drug component with a potent apoptotic effect and a potential for treating glioblastoma.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Jovanovic, Filip Grbovic, J. Milovanović, M. Nonić, M. Šijačić‐Nikolić, S. Branković
Leaf morphology is at a certain level defined by the ways in which plants adapt to different habitats, especially in large trees. In this study, morphological variations in leaf size and shape of the Hungarian oak (Quercus frainetto Ten.) growing on different soil types (lithic leptosol, vertisol, cambisol) were investigated in the central part of Serbia (Sumadija). The information on soil type was obtained using a digitalized soil map of the Republic of Serbia, while leaf traits were characterized by geometric morphometric methods. Landmark analysis and leaf measurements showed significant differences among the analyzed groups, with individuals growing on nutrient-poor, shallow soils having smaller leaves with greater lobation. The observed differences suggest that the levels of soil productivity influence variations in leaf patterns. More studies on a larger sample size and along a broader spatial scale are needed to fully understand the differences in the patterns of leaf morphological variation in Q. frainetto.
{"title":"Patterns of leaf morphological variation in Quercus frainetto Ten. growing on different soil types in Serbia","authors":"M. Jovanovic, Filip Grbovic, J. Milovanović, M. Nonić, M. Šijačić‐Nikolić, S. Branković","doi":"10.2298/abs220405018j","DOIUrl":"https://doi.org/10.2298/abs220405018j","url":null,"abstract":"Leaf morphology is at a certain level defined by the ways in which plants adapt to different habitats, especially in large trees. In this study, morphological variations in leaf size and shape of the Hungarian oak (Quercus frainetto Ten.) growing on different soil types (lithic leptosol, vertisol, cambisol) were investigated in the central part of Serbia (Sumadija). The information on soil type was obtained using a digitalized soil map of the Republic of Serbia, while leaf traits were characterized by geometric morphometric methods. Landmark analysis and leaf measurements showed significant differences among the analyzed groups, with individuals growing on nutrient-poor, shallow soils having smaller leaves with greater lobation. The observed differences suggest that the levels of soil productivity influence variations in leaf patterns. More studies on a larger sample size and along a broader spatial scale are needed to fully understand the differences in the patterns of leaf morphological variation in Q. frainetto.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhanglu An, Danyang Cai, Xiongzhi Lin, Shuaijun Xu, Jin Bin, Xiaojun Jin
The histone lysine methyltransferase SET (Suppressor of variegation, Enhancer of Zeste, Trithorax) and MYND (Myeloid-Nervy-DEAF1) domaincontaining protein (SMYD2) plays a role in the tumorigenesis of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). However, the prognostic significance of SMYD2 in CESC and the link between SMYD2 and tumor-infiltrating immune cells are unknown. The prognostic value of SMYD2 in CESC was obtained from The Cancer Genome Atlas (TCGA). SMYD2 mRNA and protein were both highly expressed in CESC compared with normal tissues. The high expression of SMYD2 was associated with advanced tumor status and poor prognosis in CESC patients. SMYD2 was an independent prognostic factor for overall survival. In vitro experiments with knockdown of SMYD2 suppressed CESC cell migration and invasion. The online tumor immune estimation resource (TIMER) and Kaplan-Meier analysis results revealed that the infiltration of CD4+ T and CD8+ T cells was related to poor prognosis. In TIMER-based multivariate Cox regression analysis, CD8+ T cells and SMYD2 were demonstrated as independent prognostic factors of CESC. In conclusion, our data suggest that high SMYD2 expression is a predictor of poor prognosis in CESC patients; SMYD2 could serve as a prognostic biomarker and molecular therapeutic target for CESC.
组蛋白lysine methyltransferase SET (Suppressor of varation, Enhancer of Zeste, Trithorax)和MYND (Myeloid-Nervy-DEAF1) domaincontaining protein, SMYD2)在宫颈鳞癌和宫颈腺癌(endocervical adencarcinoma, CESC)的发生过程中发挥重要作用。然而，SMYD2在CESC中的预后意义以及SMYD2与肿瘤浸润性免疫细胞之间的联系尚不清楚。SMYD2在CESC中的预后价值来自癌症基因组图谱(TCGA)。与正常组织相比，CESC中SMYD2 mRNA和蛋白均高表达。SMYD2的高表达与CESC患者的晚期肿瘤状态和不良预后相关。SMYD2是总体生存的独立预后因素。体外实验表明，敲除SMYD2可抑制CESC细胞的迁移和侵袭。在线肿瘤免疫估计资源(TIMER)和Kaplan-Meier分析结果显示CD4+ T和CD8+ T细胞浸润与预后不良有关。在基于timer的多变量Cox回归分析中，CD8+ T细胞和SMYD2被证明是CESC的独立预后因素。总之，我们的数据表明SMYD2高表达是CESC患者预后不良的预测因子;SMYD2可作为CESC的预后生物标志物和分子治疗靶点。
{"title":"SET and MYND domain-containing protein 2 (SMYD2): A prognostic biomarker associated with immune infiltrates in cervical squamous cell carcinoma and endocervical adenocarcinoma","authors":"Zhanglu An, Danyang Cai, Xiongzhi Lin, Shuaijun Xu, Jin Bin, Xiaojun Jin","doi":"10.2298/abs220413014a","DOIUrl":"https://doi.org/10.2298/abs220413014a","url":null,"abstract":"The histone lysine methyltransferase SET (Suppressor of variegation, Enhancer of Zeste, Trithorax) and MYND (Myeloid-Nervy-DEAF1) domaincontaining protein (SMYD2) plays a role in the tumorigenesis of cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). However, the prognostic significance of SMYD2 in CESC and the link between SMYD2 and tumor-infiltrating immune cells are unknown. The prognostic value of SMYD2 in CESC was obtained from The Cancer Genome Atlas (TCGA). SMYD2 mRNA and protein were both highly expressed in CESC compared with normal tissues. The high expression of SMYD2 was associated with advanced tumor status and poor prognosis in CESC patients. SMYD2 was an independent prognostic factor for overall survival. In vitro experiments with knockdown of SMYD2 suppressed CESC cell migration and invasion. The online tumor immune estimation resource (TIMER) and Kaplan-Meier analysis results revealed that the infiltration of CD4+ T and CD8+ T cells was related to poor prognosis. In TIMER-based multivariate Cox regression analysis, CD8+ T cells and SMYD2 were demonstrated as independent prognostic factors of CESC. In conclusion, our data suggest that high SMYD2 expression is a predictor of poor prognosis in CESC patients; SMYD2 could serve as a prognostic biomarker and molecular therapeutic target for CESC.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vladimir Sarab, V. Dragišić, Tamara Janakiev, V. Obradović, Milica Ćopić, B. Knežević, I. Dimkić
Bacterial metabarcoding analysis by 16S rDNA of five occurrences of mineral waters in Serbia (Torda, Slankamen Banja, Lomnicki Kiseljak, Velika Vrbnica and Obrenovacka Banja) indicated the presence of a high percentage of the Proteobacteria phylum, followed by the Bacteroidetes phylum. The families Rhodobacteraceae, Burkholderiaceae, Pseudomonadaceae, Methylophilaceae and Moraxellaceae were the most dominant in the bacterial flora of the selected occurrences, whereas the most represented genera were Acinetobacter, Pseudorhodobacter, Pseudomonas, Limnohabitans, Massilia, Limnobacter and Methylotenera. The presence of coliform bacteria was not detected. Alpha diversity analysis revealed that Slankamen Banja and Lomnicki Kiseljak were the richest of the selected occurrences, while the mineral waters of Torda, Velika Vrbnica and Obrenovacka Banja were characterized by similar diversity of bacterial communities determined by beta diversity analysis. Physical-chemical analysis revealed the value of total dissolved solids above 1 g/L, as well as elevated concentrations of some metals and non-metals. The research concluded that specific bacteria contribute to the development of biocorrosion and biofouling processes of water intake facilities. In addition, some of these bacteria might be potential indicators of the organic sources of pollution and/or biotechnological natural remediators in the treatment of contaminated waters.
{"title":"Bacteriome composition analysis of selected mineral water occurrences in Serbia","authors":"Vladimir Sarab, V. Dragišić, Tamara Janakiev, V. Obradović, Milica Ćopić, B. Knežević, I. Dimkić","doi":"10.2298/abs211223005s","DOIUrl":"https://doi.org/10.2298/abs211223005s","url":null,"abstract":"Bacterial metabarcoding analysis by 16S rDNA of five occurrences of mineral waters in Serbia (Torda, Slankamen Banja, Lomnicki Kiseljak, Velika Vrbnica and Obrenovacka Banja) indicated the presence of a high percentage of the Proteobacteria phylum, followed by the Bacteroidetes phylum. The families Rhodobacteraceae, Burkholderiaceae, Pseudomonadaceae, Methylophilaceae and Moraxellaceae were the most dominant in the bacterial flora of the selected occurrences, whereas the most represented genera were Acinetobacter, Pseudorhodobacter, Pseudomonas, Limnohabitans, Massilia, Limnobacter and Methylotenera. The presence of coliform bacteria was not detected. Alpha diversity analysis revealed that Slankamen Banja and Lomnicki Kiseljak were the richest of the selected occurrences, while the mineral waters of Torda, Velika Vrbnica and Obrenovacka Banja were characterized by similar diversity of bacterial communities determined by beta diversity analysis. Physical-chemical analysis revealed the value of total dissolved solids above 1 g/L, as well as elevated concentrations of some metals and non-metals. The research concluded that specific bacteria contribute to the development of biocorrosion and biofouling processes of water intake facilities. In addition, some of these bacteria might be potential indicators of the organic sources of pollution and/or biotechnological natural remediators in the treatment of contaminated waters.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible products were selected. These primers yielded 102 amplified discernible loci, of which 80 (78%) were polymorphic. A high level of genetic diversity was detected both at population and species levels; the effective number of alleles (Ne) was 1.544, the observed number of alleles (Na) was 1.784, the Nei?s genetic diversity (H) was 0.306, and Shannon's information index was 0.447. The established gene flow (Nm) was 2.329, indicating a high migration rate between the populations. A moderate level of genetic differentiation (GST: 0.176) was also observed. Analysis of molecular variance (AMOVA) revealed that 24.89% of the total genetic diversity resided among populations, while 75.10% was within the populations. Cluster analysis showed that samples from the same locality clustered together and there was no cross-clustering between the samples. The patterns of genetic variation indicate that existing C. amaena populations should be conserved.
{"title":"Assessment of the genetic diversity of a critically endangered species Centaurea amaena (Asteraceae)","authors":"B. Atasagun","doi":"10.2298/abs220826031a","DOIUrl":"https://doi.org/10.2298/abs220826031a","url":null,"abstract":"Centaurea amaena is an endemic and endangered species listed as CR (critically endangered) in Turkey. ISSR markers were used to detect the level of genetic diversity in two natural populations of C. amaena. A total of 50 ISSR primers were used and 13 primers producing polymorphic and reproducible products were selected. These primers yielded 102 amplified discernible loci, of which 80 (78%) were polymorphic. A high level of genetic diversity was detected both at population and species levels; the effective number of alleles (Ne) was 1.544, the observed number of alleles (Na) was 1.784, the Nei?s genetic diversity (H) was 0.306, and Shannon's information index was 0.447. The established gene flow (Nm) was 2.329, indicating a high migration rate between the populations. A moderate level of genetic differentiation (GST: 0.176) was also observed. Analysis of molecular variance (AMOVA) revealed that 24.89% of the total genetic diversity resided among populations, while 75.10% was within the populations. Cluster analysis showed that samples from the same locality clustered together and there was no cross-clustering between the samples. The patterns of genetic variation indicate that existing C. amaena populations should be conserved.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Kundalic, Sladjana Z. Ugrenović, I. Jovanovic, V. Petrovic, A. Petrović, Vesna Stojanovic, A. Antovic, Jasen Kundalić, I. Graovac
The aim of this study was to analyze the expression of extracellular matrix (ECM) proteins in human endoneurium during aging. We harvested 15 cadaveric sural nerves, distributed in 3 age groups (I: 25-44, II: 45-64, III: 65-86 years old). Histological sections were stained immunohistochemically for the presence of collagen type I, type IV and laminin, and the ImageJ processing program was used in morphometrical analysis to determine the percentages of these endoneurial proteins. In two younger groups, the endoneurial matrix of the sural nerve was composed from about equal proportions of these proteins, which may be considered a favorable microenvironment for the regeneration of nerve fibers. Linear regression analysis showed a significant increase in endoneurial collagen type IV with age, while collagen type I and laminin significantly decreased during the aging process. In cases older than 65 years, remodeling of the endoneurial matrix was observed to be significantly higher for the presence of collagen type IV, and lower for the expression of collagen type I and laminin. This age-related imbalance of ECM proteins could represent a disadvantageous microenvironment for nerve fiber regeneration in older adults. Our findings contribute to the development of therapeutic approaches for peripheral nerve regeneration.
{"title":"Morphometric analysis of the human endoneurial extracellular matrix components during aging","authors":"B. Kundalic, Sladjana Z. Ugrenović, I. Jovanovic, V. Petrovic, A. Petrović, Vesna Stojanovic, A. Antovic, Jasen Kundalić, I. Graovac","doi":"10.2298/ABS201214006K","DOIUrl":"https://doi.org/10.2298/ABS201214006K","url":null,"abstract":"The aim of this study was to analyze the expression of extracellular matrix (ECM) proteins in human endoneurium during aging. We harvested 15 cadaveric sural nerves, distributed in 3 age groups (I: 25-44, II: 45-64, III: 65-86 years old). Histological sections were stained immunohistochemically for the presence of collagen type I, type IV and laminin, and the ImageJ processing program was used in morphometrical analysis to determine the percentages of these endoneurial proteins. In two younger groups, the endoneurial matrix of the sural nerve was composed from about equal proportions of these proteins, which may be considered a favorable microenvironment for the regeneration of nerve fibers. Linear regression analysis showed a significant increase in endoneurial collagen type IV with age, while collagen type I and laminin significantly decreased during the aging process. In cases older than 65 years, remodeling of the endoneurial matrix was observed to be significantly higher for the presence of collagen type IV, and lower for the expression of collagen type I and laminin. This age-related imbalance of ECM proteins could represent a disadvantageous microenvironment for nerve fiber regeneration in older adults. Our findings contribute to the development of therapeutic approaches for peripheral nerve regeneration.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yingchun Qin, Yilin Xie, Aihua Li, Xiao-qin Zhang, Zhiqiang Yan
Proliferation, migration, and the phenotypic switch of vascular smooth muscle cells (VSMCs) play an important role in vascular remodeling induced by hypertension. Astragaloside IV (AS-IV), the active ingredient of Astragalus membranaceus, has been shown to exert a beneficial role in cardiovascular disease. The present study aimed to investigate the mechanism responsible for the protective effects of AS-IV on hypertension-induced vascular remodeling. AS-IV significantly reduced blood pressure and aortic media thickness in two-kidney, one-clip (2K1C) hypertensive rats. AS-IV administration downregulated the expression levels of DNA methyltransferase1 (DNMT1), matrix metalloproteinase (MMP2) and proliferating cell nuclear antigen (PCNA) and upregulated the expression of smooth muscle 22? protein (SM22?), ?-smooth muscle actin (ACTA2) and ten-eleven translocation 2 (TET2) in the aorta of hypertensive rats. AS-IV inhibited the proliferation and migration in VSMCs treated with angiotensin II (Ang II). AS-IV increased the expression of SM22?, ACTA2 and TET2, and decreased the expression of collagen Ia (COL-1a), collagen IIIa (COL-3a), DNMT1, MMP2 and PCNA in vitro. Reduction in 5-methylcytosine (5-mC) was observed in VSMCs treated with AS-IV. Knockdown of DNMT1 induced the expression of TET2, while the level of DNMT1 did not change after knockdown of TET2. These results suggest that AS-IV reversed hypertension-induced vascular remodeling by inhibiting DNMT1 and upregulating TET2.
血管平滑肌细胞(VSMCs)的增殖、迁移和表型转换在高血压诱导的血管重构中起重要作用。黄芪甲苷(Astragaloside IV, AS-IV)是黄芪的有效成分，已被证明对心血管疾病有有益作用。本研究旨在探讨AS-IV对高血压血管重构保护作用的机制。AS-IV显著降低了双肾单夹(2K1C)高血压大鼠的血压和主动脉介质厚度。AS-IV给药下调DNA甲基转移酶1 (DNMT1)、基质金属蛋白酶(MMP2)和增殖细胞核抗原(PCNA)的表达水平，上调平滑肌22?高血压大鼠主动脉中-平滑肌肌动蛋白(ACTA2)和10 - 11易位2 (TET2)的表达。AS-IV抑制血管紧张素II (Ang II)处理的VSMCs的增殖和迁移，增加SM22?， ACTA2和TET2，并降低体外Ia胶原(COL-1a)、IIIa胶原(COL-3a)、DNMT1、MMP2和PCNA的表达。AS-IV处理的VSMCs中5-甲基胞嘧啶(5-mC)减少。敲低DNMT1可诱导TET2的表达，而敲低TET2后DNMT1的表达水平未发生变化。这些结果表明，AS-IV通过抑制DNMT1和上调TET2来逆转高血压诱导的血管重构。
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Ruyi Xiao, Hanmo Feng, Xingjian Niu, Fakai Bai, Jidan Ye
Examination of the molecular mechanism of taurine regulation of lipid metabolism in fish is limited. In this study, an oleic acid (OA)-induced hepatocyte steatosis model of orange-spotted grouper (Epinephelus coioides) was established for the first time. The model was used to test the effect of taurine on steatosis hepatocytes in Control, High-fat (0.4 mM OA) and Taurine (0.4 mM OA + 2 mM taurine) experimental groups of fish. Hepatocyte samples were subjected to transcriptome analysis. A total of 99634 unigenes was assembled, 69982 unigenes were annotated and 1831 differentially expressed genes (DEGs) in Control vs High-fat group, and 526 DEGs in the High-fat vs Taurine group were identified, of which 824 DEGs (Control vs High-fat) and 237 DEGs (High-fat vs Taurine) were observed to be upregulated, and 1007 DEGs (Control vs High-fat) and 289 DEGs (High-fat vs Taurine) were downregulated after taurine intervention. These genes are involved in peroxisome proliferator-activated receptor (PPAR) and 5' AMP-activated protein kinase (AMPK) signaling pathways, fatty acid elongation, primary bile acid biosynthesis, glycerophospholipid and glycerolipid metabolism. The findings provide new clues in understanding the regulatory role of taurine in lipid and fatty acid metabolism of fish. It is hoped that the obtained results will help in the design of feed formulations to improve grouper growth from the perspective of aquaculture nutrition.
对牛磺酸调节鱼类脂质代谢的分子机制的研究是有限的。本研究首次建立了油酸诱导的橙斑石斑鱼肝细胞脂肪变性模型。采用该模型检测牛磺酸对对照组、高脂(0.4 mM OA)和牛磺酸(0.4 mM OA + 2 mM牛磺酸)试验组鱼脂肪变性肝细胞的影响。肝细胞样本进行转录组分析。共组装了99634个单基因，对69982个单基因进行了注释，鉴定出对照组与高脂组1831个差异表达基因(DEGs)，高脂与牛磺酸组526个差异表达基因(DEGs)，其中对照组与高脂组分别上调824个和237个差异表达基因(高脂与牛磺酸)，牛磺酸干预后分别下调1007个差异表达基因(对照组与高脂)和289个差异表达基因(高脂与牛磺酸)。这些基因参与过氧化物酶体增殖物活化受体(PPAR)和5' amp活化蛋白激酶(AMPK)信号通路、脂肪酸延伸、初级胆汁酸生物合成、甘油磷脂和甘油脂代谢。这些发现为了解牛磺酸在鱼类脂质和脂肪酸代谢中的调节作用提供了新的线索。希望本研究结果能从养殖营养的角度为改善石斑鱼生长的饲料配方设计提供参考。
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