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Reproduction Control in Coptodon zillii using Abrus precatorius Root Bark Diets as Fertility Inhibitor Agent 预花蒿根皮日粮作为育性抑制剂对紫齿羚繁殖的控制
Pub Date : 2021-06-12 DOI: 10.9734/ARRB/2021/V36I530381
B. Akin-Obasola
The antifertility effects of Abrus precatorius root bark meal (APRB) on Coptodon zillii juvenile was determined by a 42 days feeding experiment, with the inclusion levels of 0g, 6g, 12g, 18g and 24g/kg diet. Water volume of 20 litres in a plastic tank (50cm x 27cm x 35 cm dimension) was maintained throughout the period of experiment. Coptodon zillii was fed at 4% body weight. In male fish, testis weight, milt volume, milt count and motility duration reduced with increase in inclusion level and there were significant differences (P<0.05) in the treatments used. Histology of testis at 0g of APRB/kg diet showed primary and secondary spermatocytes in the seminiferous tubule, connective tissue was clearly seen. In treatments 6g, 12g, 18g and 24g APRB/kg diets, there were increase in interstitial cell, eroded connective tissue and necrosis. In female fish, ovary weight, fecundity, relative fecundity and gonadosomatic index reduced with increase in the concentration of treatments used. Histology of the ovary at 0g APRB/kg diet showed developing oocytes while treatments 6g, 12g, 18g and 24g revealed reduced oocytes, increase in interstitial cell, alteration in ovary development and necrosis. The research revealed that Abrus precatorius root bark meal may be effective in controlling reproduction in Coptodon zillii. Original Research Article Akin-Obasola; ARRB, 36(5): 100-106, 2021; Article no.ARRB.68159 101
通过饲粮添加水平分别为0、6、12、18、24g/kg的Abrus precatorius根皮粕(APRB),研究APRB对紫斑桡齿冬幼崽的抗生育作用。在整个实验期间,塑料水箱(尺寸为50cm x 27cm x 35cm)的水量为20升。在体重4%时饲喂紫齿象。雄鱼的睾丸重量、睾丸体积、睾丸计数和运动持续时间均随包涵水平的升高而降低,且各处理间差异显著(P<0.05)。0g APRB/kg组睾丸组织学显示精小管中有初级和次级精细胞,结缔组织清晰可见。在饲粮中添加6g、12g、18g和24g APRB后,各组间质细胞、侵蚀结缔组织和坏死增加。雌性鱼的卵巢重量、繁殖力、相对繁殖力和性腺指数随着处理浓度的增加而降低。0g APRB/kg组卵巢组织学表现为卵母细胞发育,6g、12g、18g和24g组卵巢组织学表现为卵母细胞减少、间质细胞增多、卵巢发育改变和坏死。研究表明,鲜竹根皮粉对紫齿象的繁殖有一定的控制作用。原创研究文章Akin-Obasola;植物学报,36(5):100-106,2021;文章no.ARRB。68159 101
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引用次数: 2
Spatio-temporal Brusselator Model and Biological Pattern Formation 时空Brusselator模型与生物模式形成
Pub Date : 2021-06-08 DOI: 10.9734/ARRB/2021/V36I530380
Zakir Hossine, Oishi Khanam, Md Mashih Ibn Yasin Adan, M. Kamrujjaman
This paper explores a two-species non-homogeneous reaction-diffusion model for the study of pattern formation with the Brusselator model. We scrutinize the pattern formation with initial conditions and Neumann boundary conditions in a spatially heterogeneous environment. In the whole investigation, we assume the case for random diffusion strategy. The dynamics of model behaviors show that the nature of pattern formation with varying parameters and initial conditions thoroughly. The model also studies in the absence of diffusion terms. The theoretical and numerical observations explain pattern formation using the reaction-diffusion model in both one and two dimensions. *Corresponding author: E-mail: kamrujjaman@du.ac.bd; Hossine et al.; ARRB, 36(5): 88-99, 2021; Article no.ARRB.69331
本文利用Brusselator模型研究了一种两种非均匀反应扩散模型。我们仔细研究了在空间异构环境中具有初始条件和诺伊曼边界条件的模式形成。在整个研究中,我们假设采用随机扩散策略。模型行为的动力学特性充分揭示了变参数、变初始条件下模式形成的本质。该模型还研究了没有扩散项的情况。理论和数值观测在一维和二维上解释了反应扩散模型的模式形成。*通讯作者:E-mail: kamrujjaman@du.ac.bd;Hossine等人;植物学报,36(5):88-99,2021;文章no.ARRB.69331
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引用次数: 0
Genetic Diversity Study of African Rice (Oryza glaberrima) and its Wild Relatives using Microsatellites Markers 利用微卫星标记研究非洲水稻及其野生近缘种的遗传多样性
Pub Date : 2021-06-08 DOI: 10.9734/ARRB/2021/V36I530378
D. Montcho, Mounirou Sow, Olufisayo Kolade, Roland Bocco, C. Agbangla
The Africa Rice Center Gene Bank hold about 2,500 accessions of Oryza glaberrima. To understand well the genetic diversity in O. glaberrima and its wild species, the use of molecular tools is prominent. The sample consisted of 217 accessions of O. glaberrima, 46 of O. barthii and 7 of O. sativa (checks) was genotyped with 21 polymorphic microsatellites markers. A total of 245 alleles were detected with average 11.67 alleles per locus. Number of alleles was ranged from 2 (RM124) to 20 (RM536). The polymorphic information content value was 0.49 while the heterozygosity was Original Research Article Montcho et al.; ARRB, 36(5): 75-87, 2021; Article no.ARRB.69110 76 0.091. The result showed that the sample can be clustered into four genotypic groups. Two groups among them were homogeneous. The first one consisted of O. barthii accessions with 82 alleles in total with average 3.90 alleles per locus. However, the second one consisted of only O. glaberrima accessions with 122 alleles with average 5.80 alleles per locus. O. glaberrima accessions were analyzed using model-based population structure. Results revealed two groups among O. glaberrima accessions. At the end, the identified core collection has 26 accessions consisted of 16 O. glaberrima and 10 O. barthii based on 21 microsatellites markers.
非洲水稻中心基因库保存着大约2500个glaberrima品种。为了更好地了解光刺花及其野生物种的遗传多样性,分子工具的使用是突出的。利用21个多态微卫星标记对217份黄花、46份黄花和7份黄花(对照)进行基因分型。共检测到245个等位基因,平均每个位点有11.67个等位基因。等位基因数量为2个(RM124) ~ 20个(RM536)。多态信息含量值为0.49,杂合度为moncho等人;植物学报,36(5):75-87,2021;文章no.ARRB。69110 76 0.091。结果表明,样品可聚为4个基因型群。其中两组是同质的。第1个等位基因共82个,平均每个位点有3.90个等位基因。而第2个等位基因数量为122个,平均每个位点有5.80个等位基因。利用基于模型的种群结构对黄貂鱼种群进行分析。结果表明,大叶黄属植物有两大类群。最后,基于21个微卫星标记,鉴定出了26份核心种质,其中16份为光斑草(O. glaberrima), 10份为barthii。
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引用次数: 0
Livelihood Patterns and Socio-economic Conditions of the Fishermen of Kharingcha Baor at Chaugachha Upazilla in Jashore District, Bangladesh 孟加拉国j岸上地区Chaugachha Upazilla Kharingcha Baor渔民的生计模式和社会经济条件
Pub Date : 2021-06-04 DOI: 10.9734/ARRB/2021/V36I530376
Md. Raihan Uddin, M. Rahman, M. Choudhury, Md. Almamun Farid, Anusree Biswas, M. Rahman
A field study was carried out to investigate the livelihood patterns and socio-economic conditions of the small-scale traditional fishermen of Kharingcha baor at Chaugachha upazilla in Jashore, Bangladesh. The survey was conducted on 100 peoples from 70 families of the fishermen in the study area. It was found from the study that 94% fishermen were comprising of males, while 6% were of females. Among them, the majority was Hindus (88%) and the minority was Muslims (12%). During the survey, the proportion of semi paka houses were documented to be 88%, while kancha were 12%, and all the families had electricity facilities. It was found that 20% of fishermen had no education, 6% had passed SSC-HSC examinations, 14% had passed 6-10 classes and 24% had passed 1-5 classes. It revealed that 84% of fishermen were married and 16% were unmarried. Throughout the study, 33% of the fishermen were found to be dependent on village doctors, 40% on upazilla hospital and 27% on MBBS doctors. Several types of local boats and dingi nouka as well as nets such as tana jal (surrounding net), set bagnet, khapla jal (cast net), Original Research Article Uddin et al.; ARRB, 36(5): 61-74, 2021; Article no.ARRB.68743 62 thela jal (push net) etc. were used for the harvesting of different fish species (Labeo rohita, Catla catla, Cyprinus carpio, Cirrhinus mrigala, Heteropneustes fossilis, Channa striatus, C. punctatus etc.). Averagely, 35-80 kg fish per day was caught by a group of fishermen using a boat. The majority of the fishers sold their harvested fishes upon the fulfillment of their demands. The monthly mean income of fishermen was around BDT. 5,000-15,000 in the baor area. Among the fishermen, 56% of them were observed to lend money from money lenders and 44% from the NGOs for their fishing-related activities and livelihoods. It revealed from the present findings that the traditional fishermen of Kharingcha baor require more technical, organizational and institutional helps for the improvement of their socio-economic condition and sustainable livelihood.
进行了一项实地研究,以调查孟加拉国j岸上Chaugachha upazilla的Kharingcha baor小型传统渔民的生计模式和社会经济条件。这项调查是对研究区域70个渔民家庭的100人进行的。研究发现,94%的渔民是男性,6%是女性。其中,大多数是印度教徒(88%),少数是穆斯林(12%)。在调查期间,记录的半paka房屋比例为88%,kancha为12%,所有家庭都有电力设施。调查发现,20%的渔民没有受过教育,6%的渔民通过了SSC-HSC考试,14%的渔民通过了6-10级,24%的渔民通过了1-5级。调查显示,84%的渔民已婚,16%的渔民未婚。在整个研究过程中,发现33%的渔民依赖于乡村医生,40%依赖于upazilla医院,27%依赖于MBBS医生。几种类型的当地船只和dingi nouka以及渔网,如tana jal(围网),set bagnet, khapla jal(撒网),原始研究文章Uddin等;植物学报,36(5):61- 74,2021;文章no.ARRB。采用推网等方法捕捞不同鱼类(Labeo rohita, Catla Catla, Cyprinus carpio, Cirrhinus mrigala, Heteropneustes fossilis, Channa striatus, C. punctatus等)。一群渔民平均每天用一艘船捕捞35-80公斤鱼。大多数渔民在满足他们的需求后出售他们收获的鱼。渔民的月平均收入在BDT左右。在baor地区有5000 - 15000人。在渔民中,56%的人向放债人贷款,44%的人向非政府组织贷款,用于与渔业有关的活动和生计。从目前的调查结果可以看出,哈林察坝的传统渔民需要更多的技术、组织和体制方面的帮助,以改善他们的社会经济条件和可持续的生计。
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引用次数: 0
Effects of COVID-19 Pandemic on Mental Health of Youths in Nigeria: The Counselors Intervention Strategy COVID-19大流行对尼日利亚青少年心理健康的影响:辅导员的干预策略
Pub Date : 2021-06-03 DOI: 10.9734/ARRB/2021/V36I530375
Godwin Michael Ubi, Bassey Ekeng Effiom, S. Esuabana
In late December, 2019, patients diagnosed with viral pneumonia due to an unidentified microbial agent were reported in Wuhan, China. A novel coronavirus was subsequently identified as the causative pathogen, provisionally named 2019 novel coronavirus (2019-nCoV). As at November 10, 2020, confirmed cases had reached an alarming 50,994,215 with a global death toll of 1,264,077    from 2019-nCoV infections, most of which involved people living in or visiting countries with high rate of prevalence and thus increasing the human-to-human transmission. A random sampling of 150 inmates in a Psychiatric clinic revealed that the advent of the COVID 19 pandemic in Nigeria left many youths stressed up, depressed, traumatized with excited state of anxiety leading to mental health challenges and some of which  resort to committing suicide.The post COVID 19 era plunged many youths into joblessness. Many youths who were into artisans and gainfully employed with private outfits and organizations such as private schools lost their jobs (20.67%) due to the COVID 19 disease outbreak which shut down schools, businesses and government. The state of joblessness of the youths in the current post COVID 19 era has further affected the mental health of some of the youths in Nigeria. Absence of government palliatives (4.00%), ASUU Strike (3.33%), High level of poverty, isolation, Sicknesses (13.33%), High level of Despondency, Loss of accommodation (16.67%), Inflation (increased prices of food and other items), Increased transportation cost, Persistent lockdown, Social unrest, High level of insecurity (17.33%), Security personnel brutality and Increase crime rates. The counselor’s intervention strategy should focus on helping the youths with poor mental health due to COVID 19 to regain self- confidence, regain self –esteem, seek medical attention for them, canvass for free medication for affected ones, create awareness on job opportunity for them, engage governments on behalf of the youths for employment opportunities as well as giving them palliatives, reopening of schools/universities and linking them to credit facilities, social safety nets and other available opportunities.
2019年12月下旬,中国武汉报告了因不明微生物病原体感染而被诊断为病毒性肺炎的患者。随后,一种新型冠状病毒被确定为致病病原体,暂时命名为2019新型冠状病毒(2019- ncov)。截至2020年11月10日,2019-nCoV感染的确诊病例已达到惊人的50994,215例,全球死亡人数为1,264,077人,其中大多数涉及居住或访问高流行国家的人,从而增加了人与人之间的传播。对尼日利亚一家精神病诊所150名囚犯的随机抽样调查显示,2019冠状病毒病(COVID - 19)大流行的到来,使许多年轻人压力大、情绪低落、精神受到创伤,并伴有兴奋性焦虑状态,导致心理健康挑战,其中一些人选择了自杀。新冠疫情后,很多年轻人陷入了失业。由于新冠肺炎疫情,学校、企业、政府纷纷关闭,从事手工行业并在私立学校等民间团体从事有偿工作的年轻人(20.67%)也失去了工作。在后COVID - 19时代,年轻人的失业状况进一步影响了尼日利亚一些年轻人的心理健康。缺乏政府缓解措施(4.00%),ASUU罢工(3.33%),高度贫困,孤立,疾病(13.33%),高度沮丧,失去住宿(16.67%),通货膨胀(食品和其他物品价格上涨),运输成本增加,持续封锁,社会动荡,高度不安全(17.33%),保安人员暴行和犯罪率上升。辅导员的干预策略应侧重于帮助因COVID - 19而精神健康状况不佳的青少年重拾自信,重拾自尊,为他们寻求医疗照顾,为受影响的人争取免费药物,为他们创造就业机会的意识,代表年轻人与政府接触,为他们提供就业机会,并为他们提供缓和措施,重新开放学校/大学并将他们与信贷机构联系起来,社会安全网和其他可利用的机会。
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引用次数: 0
Geographic Distribution Shift of Invasive Plant Austroeupatorium inulifolium in the Future Climate Projection 入侵植物紫茎泽兰在未来气候预测中的地理分布变化
Pub Date : 2021-05-29 DOI: 10.9734/ARRB/2021/V36I530373
A. Yudaputra, I. Fijridiyanto, I. P. Astuti, R. N. Zulkarnaen, A. Yuswandi, J. Witono, Yuzammi
Aims: This study aims to predict the future geographic distribution shift of invasive plant species Austroeupathorium inulifolium as the impact of global climate change. Study Design: The rising temperature and precipitation change lead to the geographic distribution shift of organisms. A. inulifolium belongs to invasive plant species that often causes a substantial economic loss and ecological degradation in the invaded areas. Modelling of species distribution using the climate-based model could be used to understand the geographic distribution shift of invasive species in the future scenario under global climate change. Place and Duration of Study: Center for Plant Conservation and Botanic Gardens – LIPI and 6 months. Methodology: The total 2228 of occurrence records were derived from the Global Biodiversity Original Research Article Yudaputra et al.; ARRB, 36(5): 38-47, 2021; Article no.ARRB.68482 39 Information Facility (GBIF) database. The seven climatic variables were selected from 19 variables using a pairwise correlation test (vifcor) with a threshold >0.7. The ensemble model was used by combining Random Forest (RF) and Support Vector Machine (SVM). Results: Both two models are well-performed either using AUC or TSS evaluation methods. RF and SVM have AUC >0.95, and TSS >0.8. The predicted current distribution tends to have larger distribution areas compared to observed occurrence records. The predicted future distribution seems to be shifted in several parts of North America and Europe. Conclusion: The geographic distribution of invasive plant species A. inulifolium will be shifted to the Northern part of globe in 2090. Mean temperature of driest quarter and precipitation of warmest quarter are the two most important variables that determine the distribution pattern of the A. inulifolium. The predictive distribution pattern of invasive plant A. inulifolium would be important to provide information about the impact of climate change to the geographic distribution shift of this species.
目的:本研究旨在预测全球气候变化影响下外来入侵植物白杨(Austroeupathorium inulifolium)未来地理分布的变化。研究设计:气温上升和降水变化导致生物地理分布的转移。菊属入侵植物,经常给入侵地区造成严重的经济损失和生态退化。利用基于气候的物种分布模型可以了解未来全球气候变化情景下入侵物种的地理分布变化。学习地点和时间:植物保护与植物园中心(LIPI)和6个月。方法:2228条发生记录来源于全球生物多样性原创研究文章Yudaputra et al.;植物学报,36(5):38-47,2021;文章no.ARRB。68482 39信息设施(GBIF)数据库。采用阈值>0.7的双相关检验(vifcor)从19个气候变量中筛选出7个气候变量。采用随机森林(RF)和支持向量机(SVM)相结合的集成模型。结果:无论采用AUC还是TSS评价方法,两种模型均具有良好的表现。RF和SVM的AUC >0.95, TSS >0.8。与观测到的发生记录相比,预测的电流分布往往具有更大的分布区域。预测的未来分布似乎在北美和欧洲的一些地区发生了变化。结论:2090年,入侵植物金合欢的地理分布将向地球北部转移。最干季平均气温和最暖季降水是决定金针叶分布格局的两个最重要的变量。预测入侵植物金盏花的地理分布格局将为研究气候变化对其地理分布变化的影响提供重要信息。
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引用次数: 1
Clone x Tester Crosses on Compatibility Level of Sweetpotato (Ipomoea batatas L.) 甘薯(Ipomoea batatas L.)无性系×亲和水平测试系
Pub Date : 2021-05-26 DOI: 10.9734/ARRB/2021/V36I530372
F. C. Indriani, J. Restuono, W. Rahajeng, R. Iswanto, T. Sundari, R. Soehendi, D. Harnowo, Y. Baliadi, M. J. Mejaya
Evaluation of compatibility on sweetpotato is very important to determine the crossing parents to increase the compatibility of controlled cross-pollination. This research was aimed to study the level of compatibility in controlled cross-pollination based on percentage of normal seedlings obtained from crosses. The research was conducted at Kendalpayak Experimental Station of ILETRI, Malang, Indonesia then followed by breaking seed dormancy and germination of seeds obtained. The genetic materials used were 17 accessions with high yielding potential and 3 accessions as tester for crossing. The results showed that there were differences in the compatibility levels of crossing combination and their compatibilities as male or female parents. Clone C-011 (Tester 2) was the most compatible as male parent, while clone C-141, C-007 and C-131 were the most compatible as female parents. Clone C-127 (Tester 3) was the most compatible as both male and female parents. Clone C-001 (Tester 1) could not be used as female parent due to high incompatibility.This compatible clone information is very useful in determining of both controlled crosses and open crosses in sweet potato in order to increase the breeding efficiency. Original Research Article Indriani et al.; ARRB, 36(5): 23-37, 2021; Article no.ARRB.68071 24
甘薯亲和性评价对确定杂交亲本,提高控制异花授粉的亲和性具有重要意义。本研究旨在以杂交获得的正常苗百分比为基础,研究受控异花授粉的亲和性水平。研究在印度尼西亚玛琅ILETRI Kendalpayak试验站进行,然后打破种子休眠,获得种子发芽。所选用的遗传材料为17份高产材料和3份杂交试验材料。结果表明,杂交组合的亲和水平及父母本亲和水平存在差异。无性系C-011(试验2)作为雄性亲本的亲和性最好,无性系C-141、C-007和C-131作为雌性亲本的亲和性最好。克隆C-127(测试3)作为雌雄亲本的亲和性最好。克隆C-001(测试者1)由于高度不亲和性不能用作母本。这种亲和性克隆信息对甘薯控制杂交和开放杂交的确定具有重要意义,可提高育种效率。Indriani et al.;植物学报,36(5):23- 37,2021;文章no.ARRB。68071年24
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引用次数: 2
Occurrence of Soil-transmitted Helminths from Selected Dumpsites and Farmlands in Calabar, Nigeria 尼日利亚卡拉巴尔选定垃圾场和农田土壤传播蠕虫的发生情况
Pub Date : 2021-05-25 DOI: 10.9734/ARRB/2021/V36I530371
Edema Enogiomwan Imalele, E. Offiong, U. Ukam, Aramushu Willington Urimaneh, Henshaw Victoria Utibe
Aims: The present study investigated the prevalence and intensity of soil-transmitted helminth contamination in dumpsite and farmland soils in Calabar, Cross River State, Nigeria. Methodology: Soil samples were randomly collected from selected dumpsites and farmlands from July to December 2019. 200 soil samples (100 samples each from dumpsites and farmland) were collected. Soil samples were analysed using Zinc sulphate flotation technique whereas soil nematode extraction for hookworm and Strongyloides larvae was carried out using the modified Baermann technique. Results: Of the 200 soil samples examined, 131 (65.5%) were positive for ova/larvae of one or more parasites. Dumpsite soils were highly contaminated with ova/larvae of soil-transmitted helminths than farmland soils with a prevalence of 79% (n=79) and 52% (n=52) respectively (P=.02). Farmlands had the highest mean intensity (6.75±1.88) of contamination with ova/larvae of soil-transmitted helminths compared to dumpsite soils (1.68±0.14). Ascaris lumbricoides recorded Original Research Article Imalele et al.; ARRB, 36(5): 14-22, 2021; Article no.ARRB.68607 15 the highest occurrence (35.5%) (P=.003) in soil samples examined. A. lumbricoides (40%) and Trichuris trichiura (6%) contamination was higher in dumpsite soils, while Strongyloides stercoralis larvae (34%) and hookworm (10%) contamination was higher in farmland soils. Dumpsite soils recorded the highest number of parasites (84%) in the wet season, while farmland soils recorded the highest number of parasites (92%) in the dry season (P=.11). Generally, parasitic ova/larvae were more prevalent in the dry season (91%) than in the wet season (73%) (P=.33). S. stercoralis (33%) was more prevalent in the wet season followed while A. lumbricoides (43%) recorded highest occurrence in the dry season. Conclusion: This study revealed the potential health risk of contracting soil-transmitted helminth parasites in soils around farmlands and dumpsites in Calabar, Nigeria. It is therefore important that a combination of sanitation and health education be put in place for effective control of soiltransmitted helminths.
目的:调查尼日利亚克罗斯河州卡拉巴尔市垃圾场和农田土壤中土壤传播性蠕虫污染的流行程度和强度。方法:于2019年7月至12月在选定的垃圾场和农田随机收集土壤样本。收集土壤样本200份(垃圾场和农田各100份)。土壤样品分析采用硫酸锌浮选法,土壤线虫提取采用改良Baermann法提取钩虫和圆线虫幼虫。结果:200份土壤样品中有131份(65.5%)检出一种或多种寄生虫卵/幼虫。垃圾场土壤中虫卵/幼虫的感染率高于农田土壤,分别为79% (n=79)和52% (n=52) (P= 0.02)。土壤传播蠕虫卵/幼虫污染强度(6.75±1.88)高于垃圾场土壤(1.68±0.14)。原研究文章Imalele等;植物学报,36(5):14-22,2021;文章no.ARRB。6860715在土壤样品中发病率最高,为35.5% (P= 0.003)。垃圾场土壤中蚓类蠓(40%)和毛滴虫(6%)污染程度较高,农田土壤中粪圆线虫幼虫(34%)和钩虫(10%)污染程度较高。垃圾场土壤寄生虫数量在雨季最高(84%),农田土壤寄生虫数量在旱季最高(92%)(P= 0.11)。总体而言,枯水季寄生虫卵/幼虫发生率为91%,高于丰水季(73%)(P= 0.33)。湿季以粪虫居多(33%),旱季以蚓类居多(43%)。结论:本研究揭示了尼日利亚卡拉巴尔地区农田和垃圾场周围土壤感染土壤传播寄生虫的潜在健康风险。因此,必须将卫生和健康教育结合起来,以有效控制土壤传播的蠕虫。
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引用次数: 2
Pollen Morphology of Six Indonesian Begonia (Begoniaceae) Species 6种印尼海棠属植物的花粉形态
Pub Date : 2021-05-14 DOI: 10.9734/ARRB/2021/V36I530370
H. Siregar, Sudarmono, J. Witono, Hary Wawangningrum
Aims: This study aims to determine pollen morphological differences in six Indonesian Begonia species. Study Design: All fresh pollen grain of six Indonesian Begonia species were collected using a standard method and observed at SEM (Scanning Electron Microscope) Laboratory. Place and Duration of Study: The pollen is collected from Bogor Botanic Gardens, then observed using an SEM in The Museum Zoologicum Laboratory, Research Center for Biology, Indonesian Institute of Sciences (LIPI), on August 2018. Methodology: The results of SEM pollen ultrastructure observations were analyzed descriptively by describing images of size, P/E index, aperture, endoaperture, and ornamentation of pollen. The size of pollen morphology is calculated based on the length of the polar axis and the equatorial axis on a micrometer scale. Determination of the shape of pollen can be determined by comparing the length of the polar axis with the equatorial axis. Short Communication Siregar et al.; ARRB, 36(5): 7-13, 2021; Article no.ARRB.68154 8 Results: All species examined share common pollen grain characters, i.e. isopolar and three zonocolporate. The longest polar axis (P) and equatorial diameter (E) presented by B. puspitae, whereas the shortest of P is shown by B. kudoensis and the shortest of E by B. sudjanae. Only B. hooveriana has prolate – perprolate aperture, whereas other species perprolate. Endoaperture types of B. puspitae, B. sudjanae, and B. hooveriana is lalongate, while the other species is lolongate. The coarsely striate ornamentation forms are presented by B. holosericeoides and B. natunaensis, whereas other species has fine striate ornamentation. Conclusion: The size, P/E index, aperture, endoaperture, and ornamentation of pollen are not useful for section classification of six Indonesian Begonia species. Pollen morphology should be incorporated to other characters, such as morphological, cytological, and molecular characters for making delimitation of Begonia species.
目的:研究6种印尼海棠属植物花粉形态差异。研究设计:采用标准方法采集6种印尼海棠的新鲜花粉粒,在扫描电镜实验室进行观察。研究地点和时间:从茂物植物园收集花粉,然后于2018年8月在印度尼西亚科学研究所(LIPI)生物学研究中心的博物馆动物实验室使用扫描电镜进行观察。方法:通过描述花粉的大小、P/E指数、孔径、内孔径和纹饰等图像,对SEM花粉超微结构观察结果进行描述性分析。花粉形态的大小是根据极轴和赤道轴在微米尺度上的长度来计算的。花粉的形状可以通过比较极轴和赤道轴的长度来确定。短通信系统;植物学报,36(5):7-13,2021;文章no.ARRB。结果:所有被检种均具有花粉粒特征,即等极性和三带共生。极轴(P)和赤道直径(E)最长的是puspitae,最短的是kudoensis,最短的是sudjanae。只有B. hooveriana有长-过长孔,而其他种类有过长孔。内孔型为长孔型,其他种为长孔型。全纹贝和natunaensis的纹饰形态为粗纹状,其他种的纹饰形态为细纹状。结论:6种印尼海棠属植物花粉的大小、P/E指数、孔径、内孔径和纹饰特征不能作为分类的依据。花粉形态应与其他性状,如形态、细胞学和分子特征相结合,以确定海棠种的界限。
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引用次数: 0
Determination of Free DNA (cfDNA) by RT-qPCR in Individuals in Sperm Alterations RT-qPCR法测定精子变异个体游离DNA (cfDNA)
Pub Date : 2021-05-04 DOI: 10.9734/ARRB/2021/V36I530369
M. Mbaye, Hasnae Zekhnini, B. E. Khalfi, N. Louanjli, Mustafa Zakaria, Fatiha Elmellouli, A. Soukri
Previous studies have suggested that the presence of circulating nucleic acids (cell-free DNA) in seminal plasma may indicate disease states. However, the potential association between cell-free DNA (cfDNA) levels in seminal plasma and sperm fertility parameters has not yet been definitively determined. In this study, we will compare seminal free DNA levels between normozoospermic samples and those from different pathologies related to characteristic parameters of sperm quality (asthenozoospermia, azoospermia, teratozoospermia, oligozoospermia and a few samples with a high fragmentation index) in order to detect a potential association between free DNA levels in seminal plasma and these different pathologies of male fertility. The recovery of free DNA from our different samples was done with the MACHEREY-NAGEL NucleoSpin® kit. This kit allows isolation of DNA from cell-free biological fluids using rapid silica column procedures. The quantification of free DNA in our samples was performed by quantitative PCR (RT-qPCR). Our results showed a significant difference in the level of free seminal DNA between normozoospermic samples and oligozoosperimic, teratozoosperimic, azoosperimic samples and those with a high DNA fragmentation index. On the other hand, no significant difference in the level of seminal free DNA was noted between normozoospermic and asthenozoospermic samples. These results suggest that seminal free DNA may be an important biomarker in the assessment of human sperm fertility.
以前的研究表明,在精浆中循环核酸(无细胞DNA)的存在可能指示疾病状态。然而,精浆中游离DNA (cfDNA)水平与精子生育参数之间的潜在关联尚未明确确定。在本研究中,我们将比较正常精子样本和与精子质量特征参数相关的不同病理样本(弱精子症、无精子症、畸形精子症、少精子症和少数高碎片化指数样本)的精子游离DNA水平,以检测精子血浆中游离DNA水平与这些不同男性生育能力病理之间的潜在关联。使用MACHEREY-NAGEL NucleoSpin®试剂盒从不同样品中回收游离DNA。该试剂盒允许使用快速硅胶柱程序从无细胞生物液中分离DNA。我们的样品中游离DNA采用定量PCR (RT-qPCR)进行定量。我们的研究结果表明,正常精子样本与少精子、畸形精子、无精子样本和DNA片段化指数高的样本的游离精子DNA水平有显著差异。另一方面,正常精子和弱精子样本的精子游离DNA水平无显著差异。这些结果表明,精液游离DNA可能是评估人类精子生育能力的重要生物标志物。
{"title":"Determination of Free DNA (cfDNA) by RT-qPCR in Individuals in Sperm Alterations","authors":"M. Mbaye, Hasnae Zekhnini, B. E. Khalfi, N. Louanjli, Mustafa Zakaria, Fatiha Elmellouli, A. Soukri","doi":"10.9734/ARRB/2021/V36I530369","DOIUrl":"https://doi.org/10.9734/ARRB/2021/V36I530369","url":null,"abstract":"Previous studies have suggested that the presence of circulating nucleic acids (cell-free DNA) in seminal plasma may indicate disease states. However, the potential association between cell-free DNA (cfDNA) levels in seminal plasma and sperm fertility parameters has not yet been definitively determined. \u0000In this study, we will compare seminal free DNA levels between normozoospermic samples and those from different pathologies related to characteristic parameters of sperm quality (asthenozoospermia, azoospermia, teratozoospermia, oligozoospermia and a few samples with a high fragmentation index) in order to detect a potential association between free DNA levels in seminal plasma and these different pathologies of male fertility. \u0000The recovery of free DNA from our different samples was done with the MACHEREY-NAGEL NucleoSpin® kit. This kit allows isolation of DNA from cell-free biological fluids using rapid silica column procedures. The quantification of free DNA in our samples was performed by quantitative PCR (RT-qPCR). \u0000Our results showed a significant difference in the level of free seminal DNA between normozoospermic samples and oligozoosperimic, teratozoosperimic, azoosperimic samples and those with a high DNA fragmentation index. On the other hand, no significant difference in the level of seminal free DNA was noted between normozoospermic and asthenozoospermic samples. \u0000These results suggest that seminal free DNA may be an important biomarker in the assessment of human sperm fertility.","PeriodicalId":8230,"journal":{"name":"Annual research & review in biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76866454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Annual research & review in biology
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