Pub Date : 2010-01-01DOI: 10.2376/0003-925X-61-220
S. Johler, R. Stephan
Staphylococcal Food Poisoning is one of the most prevalent causes of food-borne intoxication worldwide. It is typically self-limiting, presenting with violent vomiting following a short incubation period. Staphylococcal Food Poisoning is caused by ingestion of staphylococcal enterotoxins preformed in food. These toxins are able to cause massive T-cell proliferation and were classified as members of the pyrogenic exotoxin superantigen family. With the rise of genomic studies and the development of a wide range of new molecular techniques, research on Staphylococcal Food Poisoning is advancing quickly, revealing an immense variety of intriguing results. In this review, we focus on providing an overview of the vast field of current research on this intoxication.
{"title":"Staphylococcal food poisoning: a current review","authors":"S. Johler, R. Stephan","doi":"10.2376/0003-925X-61-220","DOIUrl":"https://doi.org/10.2376/0003-925X-61-220","url":null,"abstract":"Staphylococcal Food Poisoning is one of the most prevalent causes of food-borne intoxication worldwide. It is typically self-limiting, presenting with violent vomiting following a short incubation period. Staphylococcal Food Poisoning is caused by ingestion of staphylococcal enterotoxins preformed in food. These toxins are able to cause massive T-cell proliferation and were classified as members of the pyrogenic exotoxin superantigen family. With the rise of genomic studies and the development of a wide range of new molecular techniques, research on Staphylococcal Food Poisoning is advancing quickly, revealing an immense variety of intriguing results. In this review, we focus on providing an overview of the vast field of current research on this intoxication.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"1 1","pages":"220-228"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73404290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The proximate and fatty acid compositions of the flesh of cultured and wild common dentex (Dentex dentex), brown meagre (Sciaena umbra) and sharp-snout sea bream (Diplodus puntazzo) were evaluated. In all batches, cultured fish had higher values of fat contents. The lipids of cultured sharp-snout sea bream contained significantly (p<0.05) higher proportions of 18:1n-9cis, 20:1 n-9, 22:1 n-9, 18:2n-6cis and 22:2cis than the wild form. In addition, cultured common dentex contained significantly (p<0.05) higher proportions of 14:0, 20:1n-9, 18:2n-6cis, 20:5n-3cis and 22:6n-3. For these two species, the total polyenoic fatty acids content and the n-3/n-6 ratio were higher in the cultured than in the wild forms. Cultured brown meagre contained significantly (p<0.05) higher proportions of 14:0, 20:0, 16:1, 18:1n-9cis, 20:1n-9, 22:1 n-9, 24:1 n-9, 18:2n-6cis, 20:5n-3cis and 22:6n-3 than wild brown meagre. The total monoenoic and polyenoic fatty acid contents were higher in the cultured brown meagre, whereas the corresponding total saturated fatty acid content and the n-3/n-6 ratio were lower.
{"title":"Comparison of proximate and fatty acid composition of the flesh of wild and cultured fish species","authors":"T. Dinçer, S. Cakli, A. Cadun","doi":"10.2376/0003-925X-61-12","DOIUrl":"https://doi.org/10.2376/0003-925X-61-12","url":null,"abstract":"The proximate and fatty acid compositions of the flesh of cultured and wild common dentex (Dentex dentex), brown meagre (Sciaena umbra) and sharp-snout sea bream (Diplodus puntazzo) were evaluated. In all batches, cultured fish had higher values of fat contents. The lipids of cultured sharp-snout sea bream contained significantly (p<0.05) higher proportions of 18:1n-9cis, 20:1 n-9, 22:1 n-9, 18:2n-6cis and 22:2cis than the wild form. In addition, cultured common dentex contained significantly (p<0.05) higher proportions of 14:0, 20:1n-9, 18:2n-6cis, 20:5n-3cis and 22:6n-3. For these two species, the total polyenoic fatty acids content and the n-3/n-6 ratio were higher in the cultured than in the wild forms. Cultured brown meagre contained significantly (p<0.05) higher proportions of 14:0, 20:0, 16:1, 18:1n-9cis, 20:1n-9, 22:1 n-9, 24:1 n-9, 18:2n-6cis, 20:5n-3cis and 22:6n-3 than wild brown meagre. The total monoenoic and polyenoic fatty acid contents were higher in the cultured brown meagre, whereas the corresponding total saturated fatty acid content and the n-3/n-6 ratio were lower.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"12 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78073433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.2376/0003-925X-61-128
A. Popp, Claudia Fricker-Feer, K. Gschwend, R. Stephan
The family of Enterobacteriaceae is a useful indicator for hygiene conditions in food production facilities and food products. For powdered infant formula (PIF) the absence of Enterobacteriaceae in 100 g is required. Nevertheless, occasionally Enterobacteriaceae can be detected. A recent study concentrated on the occurrence of a specific foodborne pathogen out of the Enterobacteriaceae family, Cronobacter spp., in PIF manufacturing facilities in order to investigate clonal persistence and identify possible transmission routes. The aim of this study was to genotype isolates from frequently found other species within the family of Enterobateriaceae in order to elucidate and trace back transmission routes not only limited on Cronobacter spp. In total 216 isolates from three different species (Enterobacter (E.) cloacae, Klebsiella (K.) pneumoniae and Leclercia (L.) adecarboxylata) were genotyped. The isolates originated from raw ingredients, environment and products of an infant formula processing plant. Restriction digest with Xbal revealed discriminative PFGE patterns consisting of 10-20 bands for all three species. Heat sensitive additives could be traced back as contamination source for products. Furthermore, the production environment was found as a reservoir for persisting strains. Showing analogy to the situation described for Cronobacter spp., especially E. cloacae that can be found in the same niches as Cronobacter spp. but more frequently might therefore be used for hygiene monitoring along the processing chain. Certain genotypes of E. cloacae, that are able to persist within the factory environment, might possess special properties as e. g. enhanced desiccation tolerance enabling them to survive the harsh environmental conditions.
{"title":"PFGE-typing of Enterobacter cloacae, Klebsiella pneumoniae and Leclercia adecarboxylata isolates from an infant formula processing plant","authors":"A. Popp, Claudia Fricker-Feer, K. Gschwend, R. Stephan","doi":"10.2376/0003-925X-61-128","DOIUrl":"https://doi.org/10.2376/0003-925X-61-128","url":null,"abstract":"The family of Enterobacteriaceae is a useful indicator for hygiene conditions in food production facilities and food products. For powdered infant formula (PIF) the absence of Enterobacteriaceae in 100 g is required. Nevertheless, occasionally Enterobacteriaceae can be detected. A recent study concentrated on the occurrence of a specific foodborne pathogen out of the Enterobacteriaceae family, Cronobacter spp., in PIF manufacturing facilities in order to investigate clonal persistence and identify possible transmission routes. The aim of this study was to genotype isolates from frequently found other species within the family of Enterobateriaceae in order to elucidate and trace back transmission routes not only limited on Cronobacter spp. In total 216 isolates from three different species (Enterobacter (E.) cloacae, Klebsiella (K.) pneumoniae and Leclercia (L.) adecarboxylata) were genotyped. The isolates originated from raw ingredients, environment and products of an infant formula processing plant. Restriction digest with Xbal revealed discriminative PFGE patterns consisting of 10-20 bands for all three species. Heat sensitive additives could be traced back as contamination source for products. Furthermore, the production environment was found as a reservoir for persisting strains. Showing analogy to the situation described for Cronobacter spp., especially E. cloacae that can be found in the same niches as Cronobacter spp. but more frequently might therefore be used for hygiene monitoring along the processing chain. Certain genotypes of E. cloacae, that are able to persist within the factory environment, might possess special properties as e. g. enhanced desiccation tolerance enabling them to survive the harsh environmental conditions.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"382 1","pages":"128-131"},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80704977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Iversen, A. Lehner, Claudia Fricker-Feer, K. Gschwend, R. Stephan
Cronobacter spp. are occasional contaminants of infant food processing establishments and have been implicated in rare cases of neonatal infections. The control of these organisms during the processing of infant formula and identification of possible contaminating sources is of importance to manufacturers. In this study, infant formula processing sites were monitored for the presence of Cronobacter spp. (E. sakazakii) in 2007. Cronobacter were isolated using ISO/TS 22964 and simultaneously using a newly developed differential method, Cronobacter Screening Broth (CSB). Clonal types of Cronobacter strains in the environment, raw materials, and products were investigated using PFGE and RAPD in order to shed light on the possible dissemination routes within the facility. Over the course of the study, a total of 153 Cronobacter isolates were obtained from 46 end-products, 23 raw materials and 44 environmental samples. The 153 Cronobacter isolates represented 71 PFGE pulso-types, with 24 pulso-types containing multiple isolates. Clonal isolates present in products could be matched to indistinguishable pulso-types in raw materials and in the environment, however no strains were identified that were present in both the environment and raw materials. Although the majority of the isolates were obtained from products, the largest diversity of isolates was observed in raw materials. This may indicate raw materials as a significant source of Cronobacter entry into the production facility with subsequent selection for strains that are able to persist within the factory environment.
{"title":"Genotyping of Cronobacter (Enterobacter sakazakii) strains isolated from an infant formula processing plant.","authors":"C. Iversen, A. Lehner, Claudia Fricker-Feer, K. Gschwend, R. Stephan","doi":"10.2376/0003-925X-60-66","DOIUrl":"https://doi.org/10.2376/0003-925X-60-66","url":null,"abstract":"Cronobacter spp. are occasional contaminants of infant food processing establishments and have been implicated in rare cases of neonatal infections. The control of these organisms during the processing of infant formula and identification of possible contaminating sources is of importance to manufacturers. In this study, infant formula processing sites were monitored for the presence of Cronobacter spp. (E. sakazakii) in 2007. Cronobacter were isolated using ISO/TS 22964 and simultaneously using a newly developed differential method, Cronobacter Screening Broth (CSB). Clonal types of Cronobacter strains in the environment, raw materials, and products were investigated using PFGE and RAPD in order to shed light on the possible dissemination routes within the facility. Over the course of the study, a total of 153 Cronobacter isolates were obtained from 46 end-products, 23 raw materials and 44 environmental samples. The 153 Cronobacter isolates represented 71 PFGE pulso-types, with 24 pulso-types containing multiple isolates. Clonal isolates present in products could be matched to indistinguishable pulso-types in raw materials and in the environment, however no strains were identified that were present in both the environment and raw materials. Although the majority of the isolates were obtained from products, the largest diversity of isolates was observed in raw materials. This may indicate raw materials as a significant source of Cronobacter entry into the production facility with subsequent selection for strains that are able to persist within the factory environment.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"22 1","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82329003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2376/0003-925X-60-165
Željka Kuzmanović Grubešić, M. Hadžiosmanović, E. Elabjer, N. Zdolec
The aim of present study was to evaluate the impact of pre-slaughter resting time of Simmental fattened bulls (n=40, age 14 to 24 months, m=250-350 kg) on shelf life and ammonia level of obtained meat. Animals were transported 140 km to local slaughterhouse, within two groups. First one included farm raised animals slaughtered immediately after arrival, while the second group consisted of extensively raised animals rested 36 hours prior to slaughter. The content of ammonia was measured at 1st, 3rd, 5th, 7th and 9th day of cold storage (2– 4 o ; C) in m. longissimus dorsi and m. gracilis. Initial ammonia level as well as ammonia levels measured during the storage in both muscles were higher in group of animals slaughtered immediately after arrival comparing to group of animals rested 36 hours prior to slaughter (P<0.001). At the end of storage period, meat became rotten in both groups leading us to conclude that 36 hours of resting prior to slaughter is not long enough to sufficiently suppress negative influence of stress on shelf life of meat.
{"title":"Influence of pre-slaughter resting time on shelf life and ammonia level of young bull meat during the storage","authors":"Željka Kuzmanović Grubešić, M. Hadžiosmanović, E. Elabjer, N. Zdolec","doi":"10.2376/0003-925X-60-165","DOIUrl":"https://doi.org/10.2376/0003-925X-60-165","url":null,"abstract":"The aim of present study was to evaluate the impact of pre-slaughter resting time of Simmental fattened bulls (n=40, age 14 to 24 months, m=250-350 kg) on shelf life and ammonia level of obtained meat. Animals were transported 140 km to local slaughterhouse, within two groups. First one included farm raised animals slaughtered immediately after arrival, while the second group consisted of extensively raised animals rested 36 hours prior to slaughter. The content of ammonia was measured at 1st, 3rd, 5th, 7th and 9th day of cold storage (2– 4 o ; C) in m. longissimus dorsi and m. gracilis. Initial ammonia level as well as ammonia levels measured during the storage in both muscles were higher in group of animals slaughtered immediately after arrival comparing to group of animals rested 36 hours prior to slaughter (P<0.001). At the end of storage period, meat became rotten in both groups leading us to conclude that 36 hours of resting prior to slaughter is not long enough to sufficiently suppress negative influence of stress on shelf life of meat.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"40 1","pages":"165-167"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78719583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2376/0003-925X-60-172
S. Tähkäpää, Nina Kaario, R. Maijala, H. Korkeala, Anu Tulokas, J. Lundén
EU and national legislation constantly establishes new obligations for food business operators (FBOs). In order to study the implementation and potential problems in implementing food legislation, a questionnaire was sent to small- and medium-size meat and fish FBOs (n = 904) in Finland found in the register of the National Food Safety Authority Evira. The total response rate was 27.6%. In addition, 18 different FBOs that had gone through the process of approval for meat and fish establishments between 2005 and 2006 were interviewed.The results showed that FBOs in the meat and fish sector have both common and differing experiences of the problems caused by the food legislation requirements. Most common problems concerning food safety legislation are related to the layout of production premises and transport routes, control fees, requirements concerning in-house control, structures and maintenance of premises and varying interpretation of food safety legislation. Small FBOs saw the requirements of food legislation as more onerous than medium-sized FBOs. Small FBOs mentioned the requirements concerning in-house control and approval of the in-house control plan, labelling and traceability as problematic for their operations. On the other hand, medium-sized FBOs experienced problems relating to the requirements for building materials as such, by-products and waste management, in addition to layout of production premises and transport routes, structures and maintenance of facilities and the HACCP system.
{"title":"Problems for meat and fish business operators in implementing food law in Finland","authors":"S. Tähkäpää, Nina Kaario, R. Maijala, H. Korkeala, Anu Tulokas, J. Lundén","doi":"10.2376/0003-925X-60-172","DOIUrl":"https://doi.org/10.2376/0003-925X-60-172","url":null,"abstract":"EU and national legislation constantly establishes new obligations for food business operators (FBOs). In order to study the implementation and potential problems in implementing food legislation, a questionnaire was sent to small- and medium-size meat and fish FBOs (n = 904) in Finland found in the register of the National Food Safety Authority Evira. The total response rate was 27.6%. In addition, 18 different FBOs that had gone through the process of approval for meat and fish establishments between 2005 and 2006 were interviewed.The results showed that FBOs in the meat and fish sector have both common and differing experiences of the problems caused by the food legislation requirements. Most common problems concerning food safety legislation are related to the layout of production premises and transport routes, control fees, requirements concerning in-house control, structures and maintenance of premises and varying interpretation of food safety legislation. Small FBOs saw the requirements of food legislation as more onerous than medium-sized FBOs. Small FBOs mentioned the requirements concerning in-house control and approval of the in-house control plan, labelling and traceability as problematic for their operations. On the other hand, medium-sized FBOs experienced problems relating to the requirements for building materials as such, by-products and waste management, in addition to layout of production premises and transport routes, structures and maintenance of facilities and the HACCP system.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"316 1","pages":"172-178"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77928201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arcobacters are increasingly Isolated from food products and have been classified as emerging food pathogens In the past, they were predominantly associated with reproductive problems, mastitis and gastric ulcers in livestock, though recently they are frequently isolated from clinically healthy farm animals. In humans, Arcobacter is associated with enteritis with in some cases septicaemia. The routes of infection are Still unclear but included, person-to-person contact and consumption of contaminated water and food. At present, there is no unequivocal evidence that arcobacters are hazards for human health, but neither are they part of the normal commensal flora. The current status Of the pathogen and the importance of food contamination is reviewed.
{"title":"Arcobacter in a food safety perspective","authors":"K. Houf","doi":"10.2376/0003-925X-60-73","DOIUrl":"https://doi.org/10.2376/0003-925X-60-73","url":null,"abstract":"Arcobacters are increasingly Isolated from food products and have been classified as emerging food pathogens In the past, they were predominantly associated with reproductive problems, mastitis and gastric ulcers in livestock, though recently they are frequently isolated from clinically healthy farm animals. In humans, Arcobacter is associated with enteritis with in some cases septicaemia. The routes of infection are Still unclear but included, person-to-person contact and consumption of contaminated water and food. At present, there is no unequivocal evidence that arcobacters are hazards for human health, but neither are they part of the normal commensal flora. The current status Of the pathogen and the importance of food contamination is reviewed.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"36 1","pages":"73-76"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84729244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2376/0003-925X-60-125
M. Nevas, H. Korkeala
Food control research is a rather new field of research, focused on evaluating the efficacy and influence of food control on food safety. Food control is financed by society, which regulates needs in order to estimate the return on that investment. Does food control truly impact food safety and promote human health? Through food control research we may lay the scientific basis for the development of new control measures and policies. The occurrence of food-borne illnesses and food-related malpractices highlight the importance of an effective food control strate- gy. According to a broad literature search, the number of scientific publications in this field of research is growing, which may in part reflect growing public concern about food safety. The main areas covered in food control research today include evaluating the hazard analysis of critical control point (HACCP) systems, assessing the economic impact of implementing food control measures and evaluating national food control strategies. This overview describes the present state of food control research and discusses possible prospects in this field of research.
{"title":"Food control research: what is it all about?","authors":"M. Nevas, H. Korkeala","doi":"10.2376/0003-925X-60-125","DOIUrl":"https://doi.org/10.2376/0003-925X-60-125","url":null,"abstract":"Food control research is a rather new field of research, focused on evaluating the efficacy and influence of food control on food safety. Food control is financed by society, which regulates needs in order to estimate the return on that investment. Does food control truly impact food safety and promote human health? Through food control research we may lay the scientific basis for the development of new control measures and policies. The occurrence of food-borne illnesses and food-related malpractices highlight the importance of an effective food control strate- gy. According to a broad literature search, the number of scientific publications in this field of research is growing, which may in part reflect growing public concern about food safety. The main areas covered in food control research today include evaluating the hazard analysis of critical control point (HACCP) systems, assessing the economic impact of implementing food control measures and evaluating national food control strategies. This overview describes the present state of food control research and discusses possible prospects in this field of research.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"31 1","pages":"125-130"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86068380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2376/0003-925X-60-135
C. Zweifel, Timo Nauer, R. Stephan
Shiga toxin-producing Escherichia (E.) coli (STEC) are a major cause of foodborne diseases in humans. STEC infections have also been associated with acidified or fermented products. This fact may indicate an increased acid resistance of STEC. In the present study, four pathogenic E. coli strains (STEC 0157:H7, STEC O26:H11, STEC O159:H - , EPEC 0157:H45) and a control strain (E coli K12) were examined for growth and persistence under hydrochloric, acetic and lactic acid stress. In the growth experiments, the 0157:H45 strain (hydrochloric and acetic acid) and the 0157:H7 strain (acetic acid) showed improved acid resistance, whereas no differences were evident for the other strains. In the survival experiments, significant differences were found between strains in the stationary and logarithmic growth phase. By comparison of the three acids, acetic acid proved to be most effective for growth inhibition and inactivation. The comparison of acid adapted and non adapted STEC O157:H7, STEC O159:H - and E coli K12 showed that strains adapted with hydrochloric acid exhibited improved acid resistance, but this effect was not confirmed after lactic acid adaptation. The adaptation with acetic acid improved the acid resistance of STEC O159:H - and E. coli K12, whereas that of STEC 0157:H7 was not affected. Consequently, remarkable strain-to-strain variation in the acid stress response of "enteropathogenic" E. coli was evident. This variation was rather influenced by the acid applied than by the serotype or the pattern of virulence factors of the strains.
{"title":"Untersuchungen zum Wachstum und zur Persistenz von Escherichia coli O26:H11, O157:H7, O157:H45 und O159:H- bei Säurestress","authors":"C. Zweifel, Timo Nauer, R. Stephan","doi":"10.2376/0003-925X-60-135","DOIUrl":"https://doi.org/10.2376/0003-925X-60-135","url":null,"abstract":"Shiga toxin-producing Escherichia (E.) coli (STEC) are a major cause of foodborne diseases in humans. STEC infections have also been associated with acidified or fermented products. This fact may indicate an increased acid resistance of STEC. In the present study, four pathogenic E. coli strains (STEC 0157:H7, STEC O26:H11, STEC O159:H - , EPEC 0157:H45) and a control strain (E coli K12) were examined for growth and persistence under hydrochloric, acetic and lactic acid stress. In the growth experiments, the 0157:H45 strain (hydrochloric and acetic acid) and the 0157:H7 strain (acetic acid) showed improved acid resistance, whereas no differences were evident for the other strains. In the survival experiments, significant differences were found between strains in the stationary and logarithmic growth phase. By comparison of the three acids, acetic acid proved to be most effective for growth inhibition and inactivation. The comparison of acid adapted and non adapted STEC O157:H7, STEC O159:H - and E coli K12 showed that strains adapted with hydrochloric acid exhibited improved acid resistance, but this effect was not confirmed after lactic acid adaptation. The adaptation with acetic acid improved the acid resistance of STEC O159:H - and E. coli K12, whereas that of STEC 0157:H7 was not affected. Consequently, remarkable strain-to-strain variation in the acid stress response of \"enteropathogenic\" E. coli was evident. This variation was rather influenced by the acid applied than by the serotype or the pattern of virulence factors of the strains.","PeriodicalId":8255,"journal":{"name":"Archiv Fur Lebensmittelhygiene","volume":"32 1","pages":"135-140"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86568441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-01-01DOI: 10.2376/0003-925X-60-161
A. Bērziņš, A. Brila, S. Magone, J. Perevoščikovs, H. Korkeala
This study was undertaken to describe the trends in human listeriosis in Latvia over the 10-year period from 1998 to 2007 as a means of characterizing the epidemiological and clinical features of this disease. A total of 90 listeriosis cases were identified for the period from 1998 to 2007. During the 10-year study period the overall incidence of listeriosis was 0.4 per 100 000 of the population. Overall, 77% of all described clinical cases were characterized by meningoencephalitis and/or sepsis. The overall mortality, including abortions and perinatal infant deaths, was 6.7% (6/90). A marked clustering of listeriosis cases was observed from September to December 2000 possibly indicating one large outbreak. The lack of serotyping and molecular typing methods for subtyping of Listeria (L.) monocytogenes isolates in the present laboratory-based surveillance system is one of the main reasons why there were no officially documented listeriosis outbreaks in Latvia up to the present date. Therefore, serotyping and molecular typing should be introduced for all clinical isolates of L. monocytogenes to identify any listeriosis outbreaks as early as possible. Such measures will allow the application of appropriate actions at the food industry level, thus either preventing or significantly reducing the real burden of listeriosis in Latvia.
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