Archives of Razi Institute最新文献

Human papillomavirus (HPV) has been established as a causative agent in the development of oral squamous cell carcinoma (OSCC). Specifically, HPV types 16 and 18 are known to be prevalent in oral cancers. This cross-sectional study aimed to determine the prevalence of HPV types 16 and 18 in OSCC cases in Qazvin province, Iran. Thirty-eight paraffin-embedded samples of OSCC were selected, and DNA extraction was performed using the Roche High Pure FFPE DNA isolation kit. The quality of the extracted DNA was assessed through PCR amplification of the human β-Globin gene. The HPV detection was carried out using SYBR green-based real-time PCR with GP5+ and GP6+ primers targeting the L1 region of HPV. The HPV genotyping was conducted on positive samples using specific primers. Statistical analysis was performed between HPV infection in OSCC and age, sex, and anatomical location. This study analyzed 38 biopsy specimens obtained from male and female OSCC patients, with an average age of 64 years. Among these samples, 13 tested positive for HPV, resulting in a prevalence rate of 34.2%. The age group with the highest HPV infection rate was 61-70 (10.5%) years. Notably, HPV type 16 was detected in 21.0% of samples, HPV type 18 in 10.5%, and other viral subtypes in 2.6%. No statistically significant correlation was found between HPV prevalence and gender or age. The findings indicated that 34.2% of OSCC samples in the Qazvin province harbor HPV, with types 16 and 18 being the most common in tumors affecting the tongue. Additionally, no association was observed between HPV infection and age or gender. To address HPV as a risk factor for OSCC, public health initiatives such as vaccination, awareness campaigns, and accessible healthcare services should be implemented. They are, furthermore, incorporating HPV DNA testing into practice.

Newcastle disease is a severe viral threat to the global poultry industry due to its high prevalence and rapid transmission. Evaluating vaccination timing and effectiveness is crucial, often accomplished through the hemagglutination inhibition (HI) assay. This test relies on the virus's agglutination ability in certain animals, utilizing various inactivated antigens. Our study aimed to assess multiple Newcastle viral antigens ( LaSota, clone, thermo-resistant strain, B₁, and V₄ ) inactivated by binary ethylene amine (BEA) and formalin, seeking the best antigen and inactivator for the HI assay. We prepared the different ND antigens include; LaSota, Clone, thermo resistant, B₁, V₄ and the mixture of the antigens then inactivated them using BEA and formalin. The hemagglutination (HA) assay determined mean titers, comparing BEA and formalin inactivation. These antigens were also subjected to the HI test using 112 serum samples from different commercial poultry flocks to assess their performance. BEA-inactivated antigens exhibited significantly higher mean titers in the HA assay than formalin-inactivated antigens. In the evaluation of different antigens in the HI test, the mean titer of antigen B₁ followed by clone and LaSota displayed a higher mean titer than others. In conclusion, this study recommends using Hitchner pathotype antigens, specifically the B₁ vaccine, for Newcastle disease HI testing. BEA is the preferred inactivator, preserving antigen structure particularly the structure of hemagglutinin antigen while minimizing risks. These findings can enhance serological testing accuracy, contributing to more effective disease control and prevention in the poultry industry.

Coronavirus disease 2019 (COVID-19) infections have re-emerged in several countries due to reduced levels of antibodies provided by vaccines or the rapid emergence of viral variants, such as Alpha, Beta, Gamma, and Delta. A considerable supplementary intervention is recommended, including an additional dose of vaccination. The present study aimed to assess the predictors of the willingness to receive the third dose of the COVID-19 vaccine based on the Health Belief Model (HBM) constructs among people referring to health centers in South Khorasan province. In this cross-sectional-analytical study, 283 people over 18 years old in South Khorasan province were randomly selected using the multi-stage cluster sampling method. The data were collected using a researcher-made questionnaire, including demographic information and health status, knowledge about COVID-19, and the third dose of the vaccine, as well as a questionnaire according to the HBM constructs, which was completed through self-report methods. Finally, the data were analyzed using the Chi-square test and multivariate logistic regression analysis. The mean age of the study participants was 36.57±11.56 years (range of 18-55). The results of multivariate logistic regression analysis demonstrated that gender (P=0.012), marital status (P=0.038), occupation (P=0.013), perceived severity (P=0.005), and cues to action (P=0.018) had a significant direct effect, while perceived barriers (P=0.010) had a significant inverse effect on the willingness to accept the third dose of the vaccine. Moreover, the predictor variables explained about 67.7% of the willingness to receive the third dose of the vaccine. The current study suggested that enhancing the perceived severity of COVID-19, along with healthcare providers' recommendations to receive the vaccine and reducing perceived barriers, can effectively encourage individuals to receive the third dose of the COVID-19 vaccine. These findings can be utilized to develop interventions aimed at promoting the uptake of the booster dose of the COVID-19 vaccine.

Tegafur, gimeracil, and oteracil potassium are widely used pharmaceuticals to treat lung cancers of the gastrointestinal tract, such as those of the oral cavity, esophagus, colon and rectum, and pancreas, as well as non-small cell lung cancers. The literature review revealed that no study has yet offered a completely stability-demonstrating, validated liquid chromatography-mass spectrometric approach for the concurrent estimation of tegafur, gimeracil, and oteracil potassium, along with all known degradation products. The simultaneous detection of tegafur, gimeracil, and oteracil potassium and their forced degradation product characterization necessitated the invention of a simpler, faster, and less expensive method. Therefore, this study aimed to follow the ICH method validation standards to develop and validate a fast, easy, and rugged liquid chromatography-mass spectrometry (LC-MS) technique for the concurrent estimation of tegafur, gimeracil, and oteracil potassium in the drug substance and the finished dosage form. Tegafur, gimeracil, and oteracil potassium were examined on the Waters HPLC Alliance system, coupled to the SCIEX QTRAP 5500 mass spectrometer, and endowed with an interface capable of carrying electrospray ionization. The tegafur, gimeracil, and oteracil peaks eluted at retention times of 2.338 min, 3.756 min, and 5.338 min, respectively. The limit of detection values of tegafur, gimeracil, and oteracil were detected to be 0.6, 0.174, and 0.474 μg/mL, respectively. The results for the quantification limit were calculated at 2.0, 0.58, and 1.58 µg/mL concentrations, respectively. Tegafur, gimeracil, and oteracil had linear ranges of 50-300 µg/ml, 14.5-87 µg/ml, and 39.5-237 µg/ml, with regression coefficients of 0.99956, 0.99986, and 0.999479, respectively. The accuracy values of tegafur, gimeracil, and oteracil in the ranges of 50%, 100%, and 150% were determined at 99.9%, 99.9%, and 99.4%, respectively. The RSD for the six replicates was less than 2% for precision. According to the ICH Q2 guidelines, this approach was effectively evaluated with LC-MS to validate the chemical structures of the freshly created tegafur, gimeracil, and oteracil degradation products. An accurate and sensitive LC-MS technique was developed and validated for the concurrent quantification of tegafur, gimeracil, and oteracil potassium in the drug material and the medicinal dosage form.

Johne's disease (JD) affects domestic and wild animals across the globe. Paratuberculosis exerts huge economic impacts on the animal industry. Despite significant economic losses, little knowledge is available on the epidemiological status of Paratuberculosis in the animal population of Iran. The present study aimed to evaluate the prevalence rate of this disease in the Iranian animal population with confidence interval (CI) and p-value. The search was conducted on and screened the electronic international and national databases. Thereafter, sufficient and relevant data were extracted. Data were analyzed in STATA software (version 14). Prevalence disease rates were determined using random effect models. A total of 52 articles were included in the systematic review. According to the results, the overall disease incidence rate in Iran was 20.39%. The prevalence rate of JD was 22.33% (95% CI, 18.87-25.78) in the cattle population and 25.61% (95% CI, 21.43-29.78) in sheep. This study pinpointed that cattle and sheep were the most commonly infected hosts. The highest prevalence rate of disease was 35.88% in Tehran (95% CI, 16.77-54.99), followed by 32.86% (95% CI, 25.07-40.65), and 20.10% (95% CI, 14.63-25.58) in Khorasan Razavi and Kerman, respectively. The lowest prevalence rate of JD was 2.27% in Ilam (95% CI, 0.84-3.70). Based on this result, molecular-based methods were properly compared to other diagnostic methods. This study reported Mycobacterium avium subsp. paratuberculosis (MAP) prevalence in dairy herds in the provinces of Iran. The infection transmission from animal sources to humans and the potential role of MAP in human disease highlight a critical need for further study on this issue.

Three species of Hemiscorpius were identified in Hormozgan province, for which the available antivenoms lack the efficacy required for treating patients. Consequently, an exact identification of the existing species was deemed necessary as the first step in managing treatment procedures. Considering the morphological similarities among the species, the aim of this research was the molecular study of the samples to accurately identify the species. Hemiscorpius specimens were collected from various locations in Hormozgan province between 2021 to 2023. The Cytochrome c oxidase subunit I gene was amplified and sequenced. Four sequences were obtained from Hemiscorpius specimens collected from Hormozgan province, and three sequences were sourced from the NCBI for analysis. Bayesian inference and Maximum likelihood phylogenetic trees showed similar results, positioning the base of Hemiscorpius enischnochela tree as an older species and Hemiscorpius lepturus adjacent to Hemiscorpius acanthocercus, identified as the newest species at the tree's tip. The results confirmed the validity of three species, namely H. acanthocercus, H. enischnochela, and H. lepturus. Hemiscorpius acanthocercus and H. lepturus are known for having dangerous venom for humans with reported deaths due to their stings. Considering the importance of the members of this genus from the medical point of view, a comprehensive examination of all species is imperative.

Newcastle disease virus (NVD) from the Paramyxoviridae family is a single-stranded negative-sense RNA virus. This infection can affect both domestic poultry and almost all other bird species. It has been considered a very severe difficulty for the poultry industry all over the world. Even though it remains a potential threat to poultry industries, this virus is a powerful oncolytic virus as well. In this study, a process was accomplished to achieve concentrated and highly purified NDV V4 strain particles. Downstream processing of Newcastle virus strain V4 was characterized by amplifying virus in embryonated chicken eggs. Through a sequence of steps, harvesting allantoic fluid, clarification by centrifuge, concentration by ultrafiltration, and size exclusion separation, the reduced volume and pure virus particles were considered for the amount of ovalbumin, hemagglutinin activity, transmission electron microscopy (TEM), electrophoresis, and additionally immunogenicity of prepared antigens. The results presented a high recovery of HA activity in concentrated and purified virus with the removal of ovalbumin and the typical morphology based on TEM. Sepharose CL-4B was determined as the best media among all used resins to purify the virus. Prepared formulations as vaccines demonstrated positive hemagglutinin inhibition for 6 months and stability for 2 years. Strong evidence from organized studies supports the effectiveness of this method in concentrating and purifying intact NDV, which could be valuable in vaccine research, antiserum preparation, or even as an alternative oncotic agent to traditional methods. Despite further studies being conducted, this method can be utilized particularly on a semi-industrial scale to produce various vaccine components.

Leprosy is still found mainly in lower-middle-income countries. Breaking the chain of leprosy transmission requires various ways, especially by increasing knowledge of leprosy prevention through health education. Although several studies have mentioned the effectiveness of health education, there is still uncertainty about the best method to use. Therefore, the review question should be answered: what forms of health education have been developed about Leprosy in the community? This review study complies with the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) version 2020. The search for relevant literature involved PubMed, ScienceDirect, ERIC, Cochrane, and ProQuest databases for the studies published in the 2000 to 2023 period with the inclusion criteria such as increasing knowledge, changing attitudes and behavior, and increasing positive stigma. The quality of the study was assessed using the Critical Appraisal Skills Program, and the Risk of Bias using the Cochrane RoB tool. Overall search on databases resulted in a total of 184,681 articles. Only six studies were eligible to be included in this review. Various educational methods were used in the eligible studies, including MH Mobile, the myth or truth on Leprosy game, posters, leaflets, community meetings, tele-education the web, discussion lists, chats, activity diaries, iconographic 3D videos, classes on video streaming, video conference, case simulation, lecture, and the contact intervention (education, testimonies (direct contact), videos, and comics). All of the methods used had the potential to improve knowledge, attitude, and practice and reduce negative stigma regarding Leprosy. There is no method of health education superior to another on Leprosy prevention.

Chaya (Cnidoscolus aconitifolius) from family Euphorbiaceae is a plant has leaves similar to papaya leaves but with dark green leaf color. This plant contains phytochemical compounds such as alkaloid, flavonoid, triterpenoids, glycoside, and tannin that can function as antibacterial, antioxidant, and anti-inflammatory. Chaya leaf infusion are processed into infusion before given to the quails through drinking water, this method is used to obtain the phytochemical compounds contained in chaya leaves. This study aimed to evaluate chaya leaf infusion (Cnidoscolus aconitifolius) as a phytogenic source on the productivity and egg quality of japanese laying quail (Coturnix coturnix japonica) aged 17-20 weeks. Chaya leaf infusion is given through drinking water and is expected to increase the productivity and quality of quail egg. The variables observed consisted of productivity (water intake, feed intake, egg weigh, QDP, egg mass, FCR, mortality) and egg quality (yolk, albumen, shell weight and percentage, egg index, yolk index, albumen index, shell thickness, yolk color, and haugh unit). This study was analyzed using the T test with 2 treatments and 4 replications: T0 = drinking water without any additives and T1 = drinking water with a concentration 10% of chaya leaf infusion. The results showed that chaya leaf infusion had a significant effect (P≤0.05) on quail day production, egg mass, albumen index, and yolk color. Besides that, supplementation chaya leaf infusion significantly reduces (P<0.05) feed convertion ratio. So that the use of 10% chaya leaf infusion in drinking water by giving it 2 times a week can increase the productivity and egg quality of laying quail (Coturnix coturnix japonica) 17-20 weeks old.

Rodents act as reservoirs, intermediate hosts, or definitive hosts for various zoonotic helminths. Parasitic diseases are among the critical factors affecting the survival and composition of wild rodent populations. Wild rodents share their habitat with domestic free-grazing animals, mainly sheep, stray dogs, and cats, which allows the transmission of helminth infections, such as Toxocara and Trichinella, to these animals. This study investigated the helminth parasite fauna of wild rodents in East Azerbaijan province, north-western Iran, and discussed the possibility of parasite transmission among wild rodents. A total of 204 rodents of 17 different species (spp.) were collected in north-western Iran. Information about the genus, developmental stage, and rodent spp. was recorded for each animal. The gastrointestinal tract, liver, and diaphragm were examined for the presence of helminths. The recovered specimens were identified based on references. The prevalence rate of helminth infection among the captured rodents was 67.16%. Meriones persicus (M. persicus) showed the highest infection and diversity rates. This spp. harbored the zoonotic helminth Capillaria hepatica and some spp. belonging to the genera Toxocara, Syphacia, and Hymenolepis. M. persicus specimens from mountainous areas showed the highest infection rate. This study aimed at evaluating the potential role of wild rodent spp. as reservoirs of helminth infection in East Azerbaijan province, Iran. M. persicus was the most common spp. in our study and represented a higher proportion of the infected rodents in terms of helminth diversity and frequency. Harboring zoonotic helminths, M. persicus represents a health risk. Further studies are recommended to evaluate the prevalence of these parasites in the human community and inform people concerned about the risk of disease transmission to humans through rodents.