D. Silva, Cassia da Rosa Pires, E. D. S. Ribeiro, W. Santos, Alanna do Socorro Lima da Silva, M. D. D. C. Palha
Chelonians have, historically, played an important role as a natural resource for human food in the Amazon region, among them the Kinosternon scorpioides species, the muçuã. This is a semi-aquatic freshwater chelonian that has an omnivorous feeding habit. Due to the lack of research on the feeding of this species in captivity, the present study aimed to evaluate the acceptance and preference of fruits by K. scorpioides and to describe their feeding behavior in captivity. The study was conducted between January and June 2017, at the Scientific Breeding Ground of the Bio-Fauna Project / ISARH-UFRA. 36 muçuãs were used, 18 in the fattening phase and 18 in the brooding phase, with an average initial weight of 438g (± 16.22g) and 84g (± 16.11g), respectively. The animals were housed in polyethylene boxes with 60% of the flooded area and 40% of the dry area, with three animals per box. In order to assess food preference, fresh fruits were used, classified as regional and non-regional Amazon. Each fruit was offered in a food/animal unit for 50 minutes. Monitoring of food preference was done with the help of video cameras. It was observed that the animals in the breeding phase were those that consumed a higher percentage of regional fruits (48.89%), when compared to the fattening phase that consumed 46.67% of the same fruits. Among the fruits offered, it was possible to observe a higher consumption of pupunha (Bactris gasipaes Kunth), melon (Cucumis melo L.) and mango (Mangifera indica L.) by both groups. The regional Amazonian fruits obtained great acceptance in which the pupunha was more prominent (88.89%) for the fattening phase, however the melon (63.19%) was the most consumed by the young.
{"title":"ACEITAÇÃO DE FRUTAS AMAZÔNICAS E NÃO-AMAZÔNICAS POR MUÇUÃ, Kinosternon scorpioides (Linnaeus, 1766), EM CATIVEIRO","authors":"D. Silva, Cassia da Rosa Pires, E. D. S. Ribeiro, W. Santos, Alanna do Socorro Lima da Silva, M. D. D. C. Palha","doi":"10.5380/AVS.V26I3.80459","DOIUrl":"https://doi.org/10.5380/AVS.V26I3.80459","url":null,"abstract":"Chelonians have, historically, played an important role as a natural resource for human food in the Amazon region, among them the Kinosternon scorpioides species, the muçuã. This is a semi-aquatic freshwater chelonian that has an omnivorous feeding habit. Due to the lack of research on the feeding of this species in captivity, the present study aimed to evaluate the acceptance and preference of fruits by K. scorpioides and to describe their feeding behavior in captivity. The study was conducted between January and June 2017, at the Scientific Breeding Ground of the Bio-Fauna Project / ISARH-UFRA. 36 muçuãs were used, 18 in the fattening phase and 18 in the brooding phase, with an average initial weight of 438g (± 16.22g) and 84g (± 16.11g), respectively. The animals were housed in polyethylene boxes with 60% of the flooded area and 40% of the dry area, with three animals per box. In order to assess food preference, fresh fruits were used, classified as regional and non-regional Amazon. Each fruit was offered in a food/animal unit for 50 minutes. Monitoring of food preference was done with the help of video cameras. It was observed that the animals in the breeding phase were those that consumed a higher percentage of regional fruits (48.89%), when compared to the fattening phase that consumed 46.67% of the same fruits. Among the fruits offered, it was possible to observe a higher consumption of pupunha (Bactris gasipaes Kunth), melon (Cucumis melo L.) and mango (Mangifera indica L.) by both groups. The regional Amazonian fruits obtained great acceptance in which the pupunha was more prominent (88.89%) for the fattening phase, however the melon (63.19%) was the most consumed by the young.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43021074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. K. Valentim, Tatiana Marques Bittencourt, Heder José D' Ávilla Lima, Nayara Emanoelle Matos e Silva, Fernandes Jesuino Mukissaí Tossué, J. P. Mendes, D. P. Procopio, D. D. Moraleco
The objective of this study was to evaluate the productive performance, the efficiency of forced seedlings, and the quality of eggs of Black Avifran laying hens reared in Cage-Free System using alternative foods as an induced seedling method. The experiment lasted 56 days, divided into two phases. The first stage called the molting phase lasted 14 days, and the next phase was divided into two periods of 21 days post-molting. A total of 144 laying hens were divided into three treatments and six replicates with 8 laying hens each. The experimental treatments were: food restriction (conventional method), total feeding with ground corn and total feeding with dry grains of the distillery with corn soluble (DDGS) for seedling induction. In the seedling phase, the following were evaluated: days to cease posture, food intake, percentage of laying, and weight loss of laying hens. In the post-molting phase, egg quality, egg weight, shell weight, albumen weight, yolk weight, yolk color, yolk percentage, shell and albumen percentage, Haugh Unit, egg production, feed intake, feed conversion by mass and dozen eggs were evaluated. The use of corn and DDGS promoted a significant difference (p<0.05) only for the parameters: food intake, feed intake, and yolk color, which obtained lower values when compared to the conventional method. It is assumed that the consumption time of the ingredients (14 days) was not enough for the laying hens to achieve the expected weight loss of 25%. However, a satisfactory time was obtained to cease the posture. Corn and DDGS can be used as an alternative method to feed restriction for the induced seedling of Black Avifran laying hens reared in the Cage-Free system, as they do not alter the performance and quality of eggs in the post-molting period.
{"title":"NUTRITIONAL METHODS TO INDUCE MOLTING IN LAYING HENS IN CAGE-FREE SYSTEM","authors":"J. K. Valentim, Tatiana Marques Bittencourt, Heder José D' Ávilla Lima, Nayara Emanoelle Matos e Silva, Fernandes Jesuino Mukissaí Tossué, J. P. Mendes, D. P. Procopio, D. D. Moraleco","doi":"10.5380/avs.v26i3.75081","DOIUrl":"https://doi.org/10.5380/avs.v26i3.75081","url":null,"abstract":"The objective of this study was to evaluate the productive performance, the efficiency of forced seedlings, and the quality of eggs of Black Avifran laying hens reared in Cage-Free System using alternative foods as an induced seedling method. The experiment lasted 56 days, divided into two phases. The first stage called the molting phase lasted 14 days, and the next phase was divided into two periods of 21 days post-molting. A total of 144 laying hens were divided into three treatments and six replicates with 8 laying hens each. The experimental treatments were: food restriction (conventional method), total feeding with ground corn and total feeding with dry grains of the distillery with corn soluble (DDGS) for seedling induction. In the seedling phase, the following were evaluated: days to cease posture, food intake, percentage of laying, and weight loss of laying hens. In the post-molting phase, egg quality, egg weight, shell weight, albumen weight, yolk weight, yolk color, yolk percentage, shell and albumen percentage, Haugh Unit, egg production, feed intake, feed conversion by mass and dozen eggs were evaluated. The use of corn and DDGS promoted a significant difference (p<0.05) only for the parameters: food intake, feed intake, and yolk color, which obtained lower values when compared to the conventional method. It is assumed that the consumption time of the ingredients (14 days) was not enough for the laying hens to achieve the expected weight loss of 25%. However, a satisfactory time was obtained to cease the posture. Corn and DDGS can be used as an alternative method to feed restriction for the induced seedling of Black Avifran laying hens reared in the Cage-Free system, as they do not alter the performance and quality of eggs in the post-molting period.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70959271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veridiane Orsolin, Antonise M. Jaguezeski, Aldo Gava, M. Franca, A. Hauser, A. L. Klock, Vagner Miranda Portes, A. Thaler Neto
The indiscriminate use without criteria of nitrogen fertilization can lead to an accumulation of nitrate in pastures, animal poisoning and potential increase in residual content of this compound in milk. The objective of this study was to determine the residual levels of nitrate throughout the year in pastures and milk from small farms in western Santa Catarina (SC) that use high levels of nitrogen fertilization. The experiment was carried out from April/2018 to March/2019 on 10 dairy farms in the municipality of Riqueza (SC). Two annual collections of water samples and monthly collections of pastures and milk were carried out, in addition to obtaining information on the property and management of pasture fertilization through a structured questionnaire. Nitrate was also evaluated in pastures using the diphenylamine test. Temperature and rainfall data were obtained daily by a weather station. The average amount of nitrogen fertilizer used in the properties was 654 ± 176 kg/ha/year. The average nitrate content in the drinking water of the animals was 1.5 ± 1.4 mg/L, in the pasture it was 270 ± 76 mg/kg DM, while in milk it was 2.0 ± 0.3 mg/L. There was seasonal variation with an increase in nitrate content in pastures and milk in autumn, a period of the experiment in which low rainfall was observed. It was concluded that despite being dairy properties with high use of nitrogen fertilization, safe milk is produced in terms of nitrate levels, even in times of the year with adverse climatic conditions. The diphenylamine test has a good ability to discriminate the nitrate content in pastures and can be indicated as a quick test to verify the presence of high levels of nitrate in the pasture.
{"title":"INFLUÊNCIA DA ADUBAÇÃO NO TEOR DE NITRATO NAS PASTAGENS E NO LEITE AO LONGO DO ANO NO OESTE DE SANTA CATARINA","authors":"Veridiane Orsolin, Antonise M. Jaguezeski, Aldo Gava, M. Franca, A. Hauser, A. L. Klock, Vagner Miranda Portes, A. Thaler Neto","doi":"10.5380/AVS.V26I2.76446","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.76446","url":null,"abstract":"The indiscriminate use without criteria of nitrogen fertilization can lead to an accumulation of nitrate in pastures, animal poisoning and potential increase in residual content of this compound in milk. The objective of this study was to determine the residual levels of nitrate throughout the year in pastures and milk from small farms in western Santa Catarina (SC) that use high levels of nitrogen fertilization. The experiment was carried out from April/2018 to March/2019 on 10 dairy farms in the municipality of Riqueza (SC). Two annual collections of water samples and monthly collections of pastures and milk were carried out, in addition to obtaining information on the property and management of pasture fertilization through a structured questionnaire. Nitrate was also evaluated in pastures using the diphenylamine test. Temperature and rainfall data were obtained daily by a weather station. The average amount of nitrogen fertilizer used in the properties was 654 ± 176 kg/ha/year. The average nitrate content in the drinking water of the animals was 1.5 ± 1.4 mg/L, in the pasture it was 270 ± 76 mg/kg DM, while in milk it was 2.0 ± 0.3 mg/L. There was seasonal variation with an increase in nitrate content in pastures and milk in autumn, a period of the experiment in which low rainfall was observed. It was concluded that despite being dairy properties with high use of nitrogen fertilization, safe milk is produced in terms of nitrate levels, even in times of the year with adverse climatic conditions. The diphenylamine test has a good ability to discriminate the nitrate content in pastures and can be indicated as a quick test to verify the presence of high levels of nitrate in the pasture.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44891509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
André Luiz Carvalho Nunes da Costa, R. H. F. Teixeira, Ingrid B. Machado, Gilberto Salles Gazeta, M. Amorim
Zoological parks are an excellent source of biological material for research, as they often receive free-living wild animals. The present report discusses the occurrence of mites in the ear canal in a free-living anteater in the Metropolitan Region of Sorocaba, forwarded to the Sorocaba Zoo. During the clinical examination of the animal ectoparasites were located and collected. The mites were collected from the ear canal and were deposited in plastic pots with 70% alcohol, then sent to the National Reference Laboratory for Rickettsial Diseases Vectors (LIRN) of the Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ). Using taxonomic specific keys and with the aid of a microscope, the species Psoralges libertus Trouessart, 1896 was morphologically identified. This species was reported in another species from the Order Pilosa, but it is the first record of Psoralges libertus parasitizing a free-living Myrmecophaga tridactyla Linnaeus, 1758.
{"title":"PRIMEIRO REGISTRO DO ÁCARO Psoralges libertus Trouessart, 1896 EM CANAL AUDITIVO DE TAMANDUÁ-BANDEIRA Myrmecophaga tridactyla Linnaeus, 1758 DE VIDA LIVRE","authors":"André Luiz Carvalho Nunes da Costa, R. H. F. Teixeira, Ingrid B. Machado, Gilberto Salles Gazeta, M. Amorim","doi":"10.5380/AVS.V26I2.78981","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.78981","url":null,"abstract":"Zoological parks are an excellent source of biological material for research, as they often receive free-living wild animals. The present report discusses the occurrence of mites in the ear canal in a free-living anteater in the Metropolitan Region of Sorocaba, forwarded to the Sorocaba Zoo. During the clinical examination of the animal ectoparasites were located and collected. The mites were collected from the ear canal and were deposited in plastic pots with 70% alcohol, then sent to the National Reference Laboratory for Rickettsial Diseases Vectors (LIRN) of the Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ). Using taxonomic specific keys and with the aid of a microscope, the species Psoralges libertus Trouessart, 1896 was morphologically identified. This species was reported in another species from the Order Pilosa, but it is the first record of Psoralges libertus parasitizing a free-living Myrmecophaga tridactyla Linnaeus, 1758.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45149911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Lima, C. A. S. Sousa, J. Pederassi, João Mendes Frazão Sobrinho, C. A. S. Souza, A. N. G. Abreu-Júnior, I. A. Amorim, Joara de Sousa Andrade
The use of hypothermia for anesthesia and euthanasia in ectothermic individuals has promoted many discussions, especially in the last four decades, and yet it continues today as a method that is prohibited or restricted to certain procedures. Despite this, some studies have shown satisfactory results in obtaining anesthetic plans, raising questions about the real possibility of using this method in research. The aim of the present experiment was to determine the time patterns to generate anesthesia and low incipient lethal temperature in Phrynops geoffroanus through cooling and freezing. Turtles reached the anesthesia grade nine within 30 minutes, making ease handling due to muscle relaxation, and, in 80 minutes, they reached anesthesia grade 11, becoming insensitive. The low incipient lethal temperature (LILT) shows that 1°C is the limit freezing from where the turtle can recover its motor activities while lower temperatures cause death. The low incipient lethal temperature was determined in a 300-minute period independently of the individual's size or weight.
{"title":"THE USE OF COOLING AND FREEZING FOR ANESTHESIA OF Phrynops geoffroanus TURTLES (PLEURODIRA: CHELIDAE) AND THE ESTABLISHMENT OF LOW INCIPIENT LETHAL TEMPERATURE","authors":"M. Lima, C. A. S. Sousa, J. Pederassi, João Mendes Frazão Sobrinho, C. A. S. Souza, A. N. G. Abreu-Júnior, I. A. Amorim, Joara de Sousa Andrade","doi":"10.5380/avs.v26i2.78134","DOIUrl":"https://doi.org/10.5380/avs.v26i2.78134","url":null,"abstract":"The use of hypothermia for anesthesia and euthanasia in ectothermic individuals has promoted many discussions, especially in the last four decades, and yet it continues today as a method that is prohibited or restricted to certain procedures. Despite this, some studies have shown satisfactory results in obtaining anesthetic plans, raising questions about the real possibility of using this method in research. The aim of the present experiment was to determine the time patterns to generate anesthesia and low incipient lethal temperature in Phrynops geoffroanus through cooling and freezing. Turtles reached the anesthesia grade nine within 30 minutes, making ease handling due to muscle relaxation, and, in 80 minutes, they reached anesthesia grade 11, becoming insensitive. The low incipient lethal temperature (LILT) shows that 1°C is the limit freezing from where the turtle can recover its motor activities while lower temperatures cause death. The low incipient lethal temperature was determined in a 300-minute period independently of the individual's size or weight.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70959535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. S. Alves, E. M. Bako, Juahil Oliveira Martins Junior, Fabiana Moreira, Cristiano Twardowski, F. D. Oliveira, Jordana Ferreira Justiniano de Souza, Luciano Brüske Pinto Junior
Landrace and Large White hybrid swine females were housed in collective pens during the gestation phase and submitted to different feeding systems. In one of the housing was used the manual feeding system, with feed provided directly on the floor. In the other, the electronic feeding system in a cage with free access Gestal 3G was installed. Two groups, composed of three matrices each, were separated by housing. The females were allocated according to their Body Condition Score (ECC) and birth order, seeking the homogeneity of the lots. The objective was to evaluate the variation of Body Weight (PC), ECC and Thickness of Bacon (ET) up to 107 days of gestation. For this purpose, CP and ECC measurements were performed every two weeks. ET was evaluated each 21 days. The data were submitted to descriptive and regression analysis over time. It was observed at the end of gestation that the group housed in the pens with an electronic feeding system showed less unevenness in relation to the PC and greater control of ECC. These results, added to the reduction of hierarchical disputes and for food competition, are important for maintaining the welfare of these females. Thus, automated feeding system proved to be important allies on the creation of pregnant swine matrices in collective pens.
{"title":"CONDIÇÃO CORPORAL DE FÊMEAS SUÍNAS NA GESTAÇÃO ALOJADAS EM BAIAS COLETIVAS COM DIFERENTES SISTEMAS DE ARRAÇOAMENTO","authors":"L. S. Alves, E. M. Bako, Juahil Oliveira Martins Junior, Fabiana Moreira, Cristiano Twardowski, F. D. Oliveira, Jordana Ferreira Justiniano de Souza, Luciano Brüske Pinto Junior","doi":"10.5380/AVS.V26I2.74117","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.74117","url":null,"abstract":"Landrace and Large White hybrid swine females were housed in collective pens during the gestation phase and submitted to different feeding systems. In one of the housing was used the manual feeding system, with feed provided directly on the floor. In the other, the electronic feeding system in a cage with free access Gestal 3G was installed. Two groups, composed of three matrices each, were separated by housing. The females were allocated according to their Body Condition Score (ECC) and birth order, seeking the homogeneity of the lots. The objective was to evaluate the variation of Body Weight (PC), ECC and Thickness of Bacon (ET) up to 107 days of gestation. For this purpose, CP and ECC measurements were performed every two weeks. ET was evaluated each 21 days. The data were submitted to descriptive and regression analysis over time. It was observed at the end of gestation that the group housed in the pens with an electronic feeding system showed less unevenness in relation to the PC and greater control of ECC. These results, added to the reduction of hierarchical disputes and for food competition, are important for maintaining the welfare of these females. Thus, automated feeding system proved to be important allies on the creation of pregnant swine matrices in collective pens.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43047919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
André Luiz Bastos de Souza, M. S. Segui, L. E. Kozicki, V. Pedrosa, Rafaela Talini, R. R. Weiss, Natalia Santana Siqueira Lara
The aim of the study was to compare the reproductive efficiency of two protocols with temporary weaning (TW) of calves and one using equine chorionic gonadotropin (eCG), the synchronization of estrus and ovulation in timed artificial insemination (TAI) protocol. A total of 534 multiparous cows were distributed into three groups: temporary weaning on day 8 of the protocol group (TW8G; n = 225); temporary weaning on day 9 group (TW9G; n = 139) and equine chorionic gonadotropin group (eCGG, n = 170). TW8G received on day zero (d0) an intravaginal device with progesterone (P4; 0.558g) + estradiol benzoate (EB; 2mg, im). On d8, the device was removed with P4 + PGF (0.075mg, im) + estradiol cypionate (EC 0.5 mg, im) + removal of the calf for 48 hours. On d10, TAI was performed, and the calves returned to their mothers. The TW9G was subjected to the same protocol as the TW8G, except for the removal of the device with the P4, which occurred on the 9th, and on the d11, TAI was performed, and the calves returned to their mothers. eCGG was subjected to the same protocol as TW8G, except that on d8 it received eCG (300IU, im), on d9 estradiol benzoate and TAI on d10. The pregnancy diagnosis was made change for done at d45. The non-pregnant cows (post TAI) were re-inseminated under the observation for estrus observation (conventional AI). The remaining empty cows from conventional AI remained with the bull for another 45 days. PR after TAI was 47.1 (TW8G), 40.2 (TW9G) and 51.7% (GeCG), while PR after conventional AI was 19.3%; 31.3% and 26.8%. PR at the end of the breeding season resulted in 95.1, 90.6, and 92.3% respectively for TW8G, TW9G and eCGG. It was concluded that there was no difference in reproductive efficiency in the timed artificial insemination between the applied protocols; the use of TW or eCG can be implemented in the breeding season of beef cows.
{"title":"COMPARAÇÃO ENTRE PROTOCOLOS COM DESMAME TEMPORÁRIO VERSUS GONADOTROFINA CORIÔNICA EQUINA APÓS TRATAMENTO COM PROGESTERONA INTRAVAGINAL VISANDO A INSEMINAÇÃO ARTIFICIAL EM TEMPO FIXO EM VACAS BOS TAURUS INDICUS","authors":"André Luiz Bastos de Souza, M. S. Segui, L. E. Kozicki, V. Pedrosa, Rafaela Talini, R. R. Weiss, Natalia Santana Siqueira Lara","doi":"10.5380/AVS.V26I2.75408","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.75408","url":null,"abstract":"The aim of the study was to compare the reproductive efficiency of two protocols with temporary weaning (TW) of calves and one using equine chorionic gonadotropin (eCG), the synchronization of estrus and ovulation in timed artificial insemination (TAI) protocol. A total of 534 multiparous cows were distributed into three groups: temporary weaning on day 8 of the protocol group (TW8G; n = 225); temporary weaning on day 9 group (TW9G; n = 139) and equine chorionic gonadotropin group (eCGG, n = 170). TW8G received on day zero (d0) an intravaginal device with progesterone (P4; 0.558g) + estradiol benzoate (EB; 2mg, im). On d8, the device was removed with P4 + PGF (0.075mg, im) + estradiol cypionate (EC 0.5 mg, im) + removal of the calf for 48 hours. On d10, TAI was performed, and the calves returned to their mothers. The TW9G was subjected to the same protocol as the TW8G, except for the removal of the device with the P4, which occurred on the 9th, and on the d11, TAI was performed, and the calves returned to their mothers. eCGG was subjected to the same protocol as TW8G, except that on d8 it received eCG (300IU, im), on d9 estradiol benzoate and TAI on d10. The pregnancy diagnosis was made change for done at d45. The non-pregnant cows (post TAI) were re-inseminated under the observation for estrus observation (conventional AI). The remaining empty cows from conventional AI remained with the bull for another 45 days. PR after TAI was 47.1 (TW8G), 40.2 (TW9G) and 51.7% (GeCG), while PR after conventional AI was 19.3%; 31.3% and 26.8%. PR at the end of the breeding season resulted in 95.1, 90.6, and 92.3% respectively for TW8G, TW9G and eCGG. It was concluded that there was no difference in reproductive efficiency in the timed artificial insemination between the applied protocols; the use of TW or eCG can be implemented in the breeding season of beef cows.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41919112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anderson Demarchi Peron, Cristian André Bertuzzi, Douglas Adriano Webber, V. Holsbach, D. L. Zulpo
The enzootic bovine leukosis is a viral disease caused by the bovine leukemia virus (BLV), an oncogenic retrovirus. The disease is widespread in herds, and dairy cattle, due to the intensive management, are the most affected. The growing importance of the international cattle trade, semen, and embryos has required the sanitary certification for the BLV, and the economic impact of the enzootic bovine leukosis is mainly due to the reduction of the milk production. The aim of this study was to determine the prevalence of antibody antiBLV in dairy cattle from Toledo municipality. Fifty-eight rural properties were visited, and blood samples were collected from 390 dairy cattle. The agar gel immunodiffusion (AGID) was used to detect the antibodies against the viral agent. Of the samples analyzed, 34.4% (134/390) showed antibodies against the bovine leukosis. Positive animals were distributed in 63.9% (37/58) of the visited properties. The viral infection is widespread in dairy cattle from Toledo. Therefore, there is a need for adequate control and prevention measures for enzootic bovine leukosis. Serological tests and a constant veterinary follow-up, aiming at the gradual sanitary control of the herds, are a good tool for the control and/or eradication of the disease.
{"title":"PREVALÊNCIA DE ANTICORPOS ANTI-VÍRUS DA LEUCOSE BOVINA EM FÊMEAS DE BOVINOS LEITEIROS DE TOLEDO, PARANÁ, BRASIL","authors":"Anderson Demarchi Peron, Cristian André Bertuzzi, Douglas Adriano Webber, V. Holsbach, D. L. Zulpo","doi":"10.5380/AVS.V26I2.78396","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.78396","url":null,"abstract":"The enzootic bovine leukosis is a viral disease caused by the bovine leukemia virus (BLV), an oncogenic retrovirus. The disease is widespread in herds, and dairy cattle, due to the intensive management, are the most affected. The growing importance of the international cattle trade, semen, and embryos has required the sanitary certification for the BLV, and the economic impact of the enzootic bovine leukosis is mainly due to the reduction of the milk production. The aim of this study was to determine the prevalence of antibody antiBLV in dairy cattle from Toledo municipality. Fifty-eight rural properties were visited, and blood samples were collected from 390 dairy cattle. The agar gel immunodiffusion (AGID) was used to detect the antibodies against the viral agent. Of the samples analyzed, 34.4% (134/390) showed antibodies against the bovine leukosis. Positive animals were distributed in 63.9% (37/58) of the visited properties. The viral infection is widespread in dairy cattle from Toledo. Therefore, there is a need for adequate control and prevention measures for enzootic bovine leukosis. Serological tests and a constant veterinary follow-up, aiming at the gradual sanitary control of the herds, are a good tool for the control and/or eradication of the disease.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44794250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rute Witter, A. L. T. Melo, T. A. Pacheco, V. Dutra, L. Nakazato, C. S. Chitarra, A. C. S. Oliveira, D. M. Aguiar, R. Pacheco
Ehrlichia minasensis, a novel species of Ehrlichia that is closely related to E. canis, is known to infect cattle and deer in Canada. This rickettsial bacterium was isolated from Rhipicephalus (Boophilus) microplus ticks and from dairy and beef cattle in southeast and midwestern Brazil, respectively. The purpose of this study was to determine the seroprevalence and perform molecular detection of Ehrlichia sp. in dairy cattle in the northern region of Brazil. The study was conducted in the municipality of Ji-Paraná, located in the state of Rondônia in the western Brazilian Amazon region. Blood and serum samples were obtained between the dates September 2012 and November 2013 from dairy cows (≥ 24 months old) in 64 farms. The blood samples were subjected to polymerase chain reaction (PCR) to amplify a fragment of the Ehrlichia sp. dsb gene, and the levels of Ehrlichia sp. antibodies were measured by the immunofluorescence assay (IFA). A total of 15 of 610 (2.45%; 95% CI: 1.04-3.86%) blood samples tested positive for ehrlichial infection based on the detection of the Ehrlichia sp. dsb gene. Sequencing of PCR amplicons from samples confirmed that the amplified partial dsb gene (~295 base pairs) sequence represented E. minasensis. Anti-Ehrlichia sp. antibodies were detected in 178 cows (53.96%; 95% CI: 46.63–61.29%). Endpoint titers ranged from 40 to 5,120. However, positive results derived from this assay should be interpreted with caution. Among the variables analyzed using IFA, the number of cows aged 24 months or greater was statistically significant (p = 0.0103), and hers of approximately 51-100 cows were more likely to be infected with Ehrlichia sp. Although the animals do not show clinical disease, the chronic character of the infection can lead to decrease in productivity.
{"title":"MOLECULAR DETECTION AND SEROPREVALENCE OF Ehrlichia sp. IN DAIRY CATTLE FROM BRAZIL’S WESTERN AMAZON REGION","authors":"Rute Witter, A. L. T. Melo, T. A. Pacheco, V. Dutra, L. Nakazato, C. S. Chitarra, A. C. S. Oliveira, D. M. Aguiar, R. Pacheco","doi":"10.5380/AVS.V26I2.74784","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.74784","url":null,"abstract":"Ehrlichia minasensis, a novel species of Ehrlichia that is closely related to E. canis, is known to infect cattle and deer in Canada. This rickettsial bacterium was isolated from Rhipicephalus (Boophilus) microplus ticks and from dairy and beef cattle in southeast and midwestern Brazil, respectively. The purpose of this study was to determine the seroprevalence and perform molecular detection of Ehrlichia sp. in dairy cattle in the northern region of Brazil. The study was conducted in the municipality of Ji-Paraná, located in the state of Rondônia in the western Brazilian Amazon region. Blood and serum samples were obtained between the dates September 2012 and November 2013 from dairy cows (≥ 24 months old) in 64 farms. The blood samples were subjected to polymerase chain reaction (PCR) to amplify a fragment of the Ehrlichia sp. dsb gene, and the levels of Ehrlichia sp. antibodies were measured by the immunofluorescence assay (IFA). A total of 15 of 610 (2.45%; 95% CI: 1.04-3.86%) blood samples tested positive for ehrlichial infection based on the detection of the Ehrlichia sp. dsb gene. Sequencing of PCR amplicons from samples confirmed that the amplified partial dsb gene (~295 base pairs) sequence represented E. minasensis. Anti-Ehrlichia sp. antibodies were detected in 178 cows (53.96%; 95% CI: 46.63–61.29%). Endpoint titers ranged from 40 to 5,120. However, positive results derived from this assay should be interpreted with caution. Among the variables analyzed using IFA, the number of cows aged 24 months or greater was statistically significant (p = 0.0103), and hers of approximately 51-100 cows were more likely to be infected with Ehrlichia sp. Although the animals do not show clinical disease, the chronic character of the infection can lead to decrease in productivity.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48272641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juliano Biolchi, Giane Helenita Pontarolo, Daniele de Cassia Karvat, D. Pedrassani
The opossums are synanthropic mammals belonging to the genus Didelphis. They have a generalized diet, and this behavior exposes them to the chance of infection by several parasitic agents. Their close relationship with the human makes them act as disseminators of pathogens. The objective of the study was to evaluate the diversity of endoparasites infecting D. albiventris sampled in Canoinhas, Northern Santa Catarina. Thirty-eigth fecal samples were macro and microscopically analyzed, and of these 89.47% showed positivity for at least one endoparasite, 89.47% (34/38) for helminths and 60.52% (23/38) for protozoa. Nematode eggs of the order Strongylida and the genera Cruzia, Trichuris, Aspidodera, Physaloptera, Capillaria and Strongyloides were observed, as well as trematode eggs and oocysts of protozoa. In 38.3% of the animals four parasite types were detected, showing high occurrence of endoparasites and that multiparasitism is frequent in this species.
{"title":"ANÁLISE COPROPARASITOLÓGICA DE GAMBÁS-DE-ORELHA-BRANCA PERTENCENTES A ÁREAS URBANAS E RURAIS DO MUNICÍPIO DE CANOINHAS, NORTE DE SANTA CATARINA","authors":"Juliano Biolchi, Giane Helenita Pontarolo, Daniele de Cassia Karvat, D. Pedrassani","doi":"10.5380/AVS.V26I2.76985","DOIUrl":"https://doi.org/10.5380/AVS.V26I2.76985","url":null,"abstract":"The opossums are synanthropic mammals belonging to the genus Didelphis. They have a generalized diet, and this behavior exposes them to the chance of infection by several parasitic agents. Their close relationship with the human makes them act as disseminators of pathogens. The objective of the study was to evaluate the diversity of endoparasites infecting D. albiventris sampled in Canoinhas, Northern Santa Catarina. Thirty-eigth fecal samples were macro and microscopically analyzed, and of these 89.47% showed positivity for at least one endoparasite, 89.47% (34/38) for helminths and 60.52% (23/38) for protozoa. Nematode eggs of the order Strongylida and the genera Cruzia, Trichuris, Aspidodera, Physaloptera, Capillaria and Strongyloides were observed, as well as trematode eggs and oocysts of protozoa. In 38.3% of the animals four parasite types were detected, showing high occurrence of endoparasites and that multiparasitism is frequent in this species.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47324556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}