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ACEITAÇÃO DE FRUTAS AMAZÔNICAS E NÃO-AMAZÔNICAS POR MUÇUÃ, Kinosternon scorpioides (Linnaeus, 1766), EM CATIVEIRO 被囚禁的mucua, Kinosternon scorpioides (Linnaeus, 1766)接受亚马逊和非亚马逊水果
Q4 Veterinary Pub Date : 2021-09-29 DOI: 10.5380/AVS.V26I3.80459
D. Silva, Cassia da Rosa Pires, E. D. S. Ribeiro, W. Santos, Alanna do Socorro Lima da Silva, M. D. D. C. Palha
Chelonians have, historically, played an important role as a natural resource for human food in the Amazon region, among them the Kinosternon scorpioides species, the muçuã. This is a semi-aquatic freshwater chelonian that has an omnivorous feeding habit. Due to the lack of research on the feeding of this species in captivity, the present study aimed to evaluate the acceptance and preference of fruits by K. scorpioides and to describe their feeding behavior in captivity. The study was conducted between January and June 2017, at the Scientific Breeding Ground of the Bio-Fauna Project / ISARH-UFRA. 36 muçuãs were used, 18 in the fattening phase and 18 in the brooding phase, with an average initial weight of 438g (± 16.22g) and 84g (± 16.11g), respectively. The animals were housed in polyethylene boxes with 60% of the flooded area and 40% of the dry area, with three animals per box. In order to assess food preference, fresh fruits were used, classified as regional and non-regional Amazon. Each fruit was offered in a food/animal unit for 50 minutes. Monitoring of food preference was done with the help of video cameras. It was observed that the animals in the breeding phase were those that consumed a higher percentage of regional fruits (48.89%), when compared to the fattening phase that consumed 46.67% of the same fruits. Among the fruits offered, it was possible to observe a higher consumption of pupunha (Bactris gasipaes Kunth), melon (Cucumis melo L.) and mango (Mangifera indica L.) by both groups. The regional Amazonian fruits obtained great acceptance in which the pupunha was more prominent (88.89%) for the fattening phase, however the melon (63.19%) was the most consumed by the young.
历史上,龟类动物在亚马逊地区作为人类食物的自然资源发挥了重要作用,其中包括Kinosternon scorpioides物种muçuã。这是一种半水生淡水龟,具有杂食性。由于对该物种的圈养摄食研究不足,本研究旨在评估蝎蠓对果实的接受和偏好,并描述其圈养摄食行为。该研究于2017年1月至6月在生物动物项目/ ISARH-UFRA的科学繁殖地进行。育肥期18头,育雏期18头,平均初始体重分别为438g(±16.22g)和84g(±16.11g),共36头。这些动物被安置在聚乙烯箱中,其中60%的水淹区和40%的干燥区,每箱3只动物。为了评估食物偏好,使用新鲜水果,将其分为区域和非区域亚马逊。每种水果在食物/动物单元中放置50分钟。对食物偏好的监测是在摄像机的帮助下完成的。结果表明,繁殖期食用区域水果的比例(48.89%)高于育肥期食用区域水果的比例(46.67%)。在提供的水果中,有可能观察到两组对pupunha (Bactris gasipaes Kunth),甜瓜(Cucumis melo L.)和芒果(Mangifera indica L.)的消耗更高。区域亚马孙水果的接受度较高,育肥期以果皮为主(88.89%),幼龄期以甜瓜为主(63.19%)。
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引用次数: 0
NUTRITIONAL METHODS TO INDUCE MOLTING IN LAYING HENS IN CAGE-FREE SYSTEM 非笼养条件下蛋鸡诱导换壳的营养方法
Q4 Veterinary Pub Date : 2021-09-29 DOI: 10.5380/avs.v26i3.75081
J. K. Valentim, Tatiana Marques Bittencourt, Heder José D' Ávilla Lima, Nayara Emanoelle Matos e Silva, Fernandes Jesuino Mukissaí Tossué, J. P. Mendes, D. P. Procopio, D. D. Moraleco
The objective of this study was to evaluate the productive performance, the efficiency of forced seedlings, and the quality of eggs of Black Avifran laying hens reared in Cage-Free  System using alternative foods as an induced seedling method. The experiment lasted 56 days, divided into two phases. The first stage called the molting phase lasted 14 days, and the next phase was divided into two periods of 21 days post-molting. A total of 144 laying hens were divided into three treatments and six replicates with 8 laying hens each. The experimental treatments were: food restriction (conventional method), total feeding with ground corn and total feeding with dry grains of the distillery with corn soluble (DDGS) for seedling induction. In the seedling phase, the following were evaluated: days to cease posture, food intake, percentage of laying, and weight loss of laying hens. In the post-molting phase, egg quality, egg weight, shell weight, albumen weight, yolk weight, yolk color, yolk percentage, shell and albumen percentage, Haugh Unit, egg production, feed intake, feed conversion by mass and dozen eggs were evaluated. The use of corn and DDGS promoted a significant difference (p<0.05) only for the parameters: food intake, feed intake, and yolk color, which obtained lower values when compared to the conventional method. It is assumed that the consumption time of the ingredients (14 days) was not enough for the laying hens to achieve the expected weight loss of 25%. However, a satisfactory time was obtained to cease the posture. Corn and DDGS can be used as an alternative method to feed restriction for the induced seedling of Black Avifran laying hens reared in the Cage-Free system, as they do not alter the performance and quality of eggs in the post-molting period.
本试验旨在评价替代食物诱导育苗法在散养体系中饲养的黑威弗兰蛋鸡的生产性能、强制育苗效率和鸡蛋质量。试验期56 d,分为两个阶段。第一阶段为蜕皮期,蜕皮期持续14 d,第二阶段为蜕皮后21 d的两个阶段。试验选用144只蛋鸡,分为3个处理,6个重复,每个重复8只鸡。试验处理分别为:限食(常规法)、全饲玉米粉和全饲酒厂干粒用玉米可溶性物(DDGS)诱导成苗。在苗期,对蛋鸡停止姿势的天数、采食量、产蛋率和体重减轻进行了评价。在蜕皮后阶段,对蛋品质、蛋重、壳重、蛋白重、蛋黄重、蛋黄颜色、蛋黄率、壳蛋白率、哈夫单位、产蛋量、采食量、质量饲料系数和打蛋进行评价。玉米和DDGS的使用对采食量、采食量和蛋黄颜色有显著影响(p<0.05),且均低于常规方法。假设这些原料消耗时间(14 d)不足以使蛋鸡达到预期减重25%的目标。然而,一个令人满意的时间是获得停止姿势。玉米和DDGS可作为非笼养黑威朗蛋鸡诱导苗限饲的替代方法,因为它们不会改变脱壳后鸡蛋的性能和质量。
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引用次数: 0
INFLUÊNCIA DA ADUBAÇÃO NO TEOR DE NITRATO NAS PASTAGENS E NO LEITE AO LONGO DO ANO NO OESTE DE SANTA CATARINA 圣卡塔琳娜西部地区全年施肥对牧草和牛奶硝酸盐含量的影响
Q4 Veterinary Pub Date : 2021-06-30 DOI: 10.5380/AVS.V26I2.76446
Veridiane Orsolin, Antonise M. Jaguezeski, Aldo Gava, M. Franca, A. Hauser, A. L. Klock, Vagner Miranda Portes, A. Thaler Neto
The indiscriminate use without criteria of nitrogen fertilization can lead to an accumulation of nitrate in pastures, animal poisoning and potential increase in residual content of this compound in milk. The objective of this study was to determine the residual levels of nitrate throughout the year in pastures and milk from small farms in western Santa Catarina (SC) that use high levels of nitrogen fertilization. The experiment was carried out from April/2018 to March/2019 on 10 dairy farms in the municipality of Riqueza (SC). Two annual collections of water samples and monthly collections of pastures and milk were carried out, in addition to obtaining information on the property and management of pasture fertilization through a structured questionnaire. Nitrate was also evaluated in pastures using the diphenylamine test. Temperature and rainfall data were obtained daily by a weather station. The average amount of nitrogen fertilizer used in the properties was 654 ± 176 kg/ha/year. The average nitrate content in the drinking water of the animals was 1.5 ± 1.4 mg/L, in the pasture it was 270 ± 76 mg/kg DM, while in milk it was 2.0 ± 0.3 mg/L. There was seasonal variation with an increase in nitrate content in pastures and milk in autumn, a period of the experiment in which low rainfall was observed. It was concluded that despite being dairy properties with high use of nitrogen fertilization, safe milk is produced in terms of nitrate levels, even in times of the year with adverse climatic conditions. The diphenylamine test has a good ability to discriminate the nitrate content in pastures and can be indicated as a quick test to verify the presence of high levels of nitrate in the pasture.
不按标准施用氮肥会导致硝酸盐在牧场的积累,动物中毒,并可能增加该化合物在牛奶中的残留含量。本研究的目的是确定圣卡塔琳娜西部(SC)使用高水平氮肥的小型农场的牧场和牛奶全年的硝酸盐残留水平。该实验于2018年4月至2019年3月在里克萨市(SC)的10个奶牛场进行。除了通过一份结构调查表获得关于牧场施肥的财产和管理的资料外,还进行了两次年度水样收集和每月牧场和牛奶收集。利用二苯胺试验对牧草硝酸盐含量进行了评价。气温和雨量资料由气象站每日取得。氮肥平均施用量为654±176 kg/ha/年。动物饮用水中硝酸盐平均含量为1.5±1.4 mg/L,牧场中为270±76 mg/kg DM,牛奶中为2.0±0.3 mg/L。牧草和牛奶中硝酸盐含量有季节性变化,秋季是降雨较少的试验期。由此得出结论,尽管乳制品中大量使用氮肥,但就硝酸盐水平而言,即使在一年中的不利气候条件下,也能生产出安全的牛奶。二苯胺试验对牧草中硝酸盐含量的判别能力较好,可作为一种快速判别牧草中硝酸盐含量过高的试验方法。
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引用次数: 0
PRIMEIRO REGISTRO DO ÁCARO Psoralges libertus Trouessart, 1896 EM CANAL AUDITIVO DE TAMANDUÁ-BANDEIRA Myrmecophaga tridactyla Linnaeus, 1758 DE VIDA LIVRE ACAR Psoralges libertus Trouesart的首次注册,1896年在坦曼杜阿-班代拉Myrmcephaga tridactyla Linnaeus的听觉通道中,1758年自由生活
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.78981
André Luiz Carvalho Nunes da Costa, R. H. F. Teixeira, Ingrid B. Machado, Gilberto Salles Gazeta, M. Amorim
Zoological parks are an excellent source of biological material for research, as they often receive free-living wild animals. The present report discusses the occurrence of mites in the ear canal in a free-living anteater in the Metropolitan Region of Sorocaba, forwarded to the Sorocaba Zoo. During the clinical examination of the animal ectoparasites were located and collected. The mites were collected from the ear canal and were deposited in plastic pots with 70% alcohol, then sent to the National Reference Laboratory for Rickettsial Diseases Vectors (LIRN) of the Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (FIOCRUZ). Using taxonomic specific keys and with the aid of a microscope, the species Psoralges libertus Trouessart, 1896 was morphologically identified. This species was reported in another species from the Order Pilosa, but it is the first record of Psoralges libertus parasitizing a free-living Myrmecophaga tridactyla Linnaeus, 1758.
动物公园是研究生物材料的绝佳来源,因为它们经常接纳自由生活的野生动物。本报告讨论了索罗卡巴大都会区一只自由生活食蚁兽耳道中螨虫的发生情况,并转发给索罗卡巴动物园。在对动物进行临床检查期间,对体外寄生虫进行了定位和收集。从耳道收集螨虫,并将其存放在含有70%酒精的塑料罐中,然后送往奥斯瓦尔多·克鲁兹研究所(IOC)的国家立克次体病媒参考实验室(LIRN)、奥斯瓦尔多·克鲁斯基金会(FIOCRUZ)。利用分类学上的特殊键,在显微镜的帮助下,对Psoralges libertus Trouessart,1896进行了形态鉴定。该物种在Pilosa目的另一个物种中也有报道,但这是1758年第一次记录到Psoralges libertus寄生在自由生活的Myrmcephaga tridactyla Linnaeus身上。
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引用次数: 0
THE USE OF COOLING AND FREEZING FOR ANESTHESIA OF Phrynops geoffroanus TURTLES (PLEURODIRA: CHELIDAE) AND THE ESTABLISHMENT OF LOW INCIPIENT LETHAL TEMPERATURE 冷却和冷冻麻醉对黄颡鱼龟(羽龟科)的应用及早期低致死温度的建立
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/avs.v26i2.78134
M. Lima, C. A. S. Sousa, J. Pederassi, João Mendes Frazão Sobrinho, C. A. S. Souza, A. N. G. Abreu-Júnior, I. A. Amorim, Joara de Sousa Andrade
The use of hypothermia for anesthesia and euthanasia in ectothermic individuals has promoted many discussions, especially in the last four decades, and yet it continues today as a method that is prohibited or restricted to certain procedures. Despite this, some studies have shown satisfactory results in obtaining anesthetic plans, raising questions about the real possibility of using this method in research. The aim of the present experiment was to determine the time patterns to generate anesthesia and low incipient lethal temperature in Phrynops geoffroanus through cooling and freezing. Turtles reached the anesthesia grade nine within 30 minutes, making ease handling due to muscle relaxation, and, in 80 minutes, they reached anesthesia grade 11, becoming insensitive. The low incipient lethal temperature (LILT) shows that 1°C is the limit freezing from where the turtle can recover its motor activities while lower temperatures cause death. The low incipient lethal temperature was determined in a 300-minute period independently of the individual's size or weight.
在过去的四十年中,对失温患者使用低温麻醉和安乐死已经引起了许多讨论,然而直到今天,它仍然是一种被禁止或限制在某些程序中的方法。尽管如此,一些研究在获得麻醉方案方面已经显示出令人满意的结果,这就提出了关于在研究中使用这种方法的真正可能性的问题。本实验的目的是确定通过冷却和冷冻使黄菖蒲产生麻醉和低致死温度的时间模式。海龟在30分钟内达到麻醉9级,由于肌肉松弛,可以轻松处理,80分钟后,它们达到麻醉11级,变得麻木不仁。低起始致死温度(LILT)表明,1°C是海龟可以恢复运动活动的极限冻结,而较低的温度会导致死亡。低起始致死温度是在300分钟内确定的,与个体的大小或体重无关。
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引用次数: 0
CONDIÇÃO CORPORAL DE FÊMEAS SUÍNAS NA GESTAÇÃO ALOJADAS EM BAIAS COLETIVAS COM DIFERENTES SISTEMAS DE ARRAÇOAMENTO 不同贮存系统的集体饲养管理中猪女性的身体状况
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.74117
L. S. Alves, E. M. Bako, Juahil Oliveira Martins Junior, Fabiana Moreira, Cristiano Twardowski, F. D. Oliveira, Jordana Ferreira Justiniano de Souza, Luciano Brüske Pinto Junior
Landrace and Large White hybrid swine females were housed in collective pens during the gestation phase and submitted to different feeding systems. In one of the housing was used the manual feeding system, with feed provided directly on the floor. In the other, the electronic feeding system in a cage with free access Gestal 3G was installed. Two groups, composed of three matrices each, were separated by housing. The females were allocated according to their Body Condition Score (ECC) and birth order, seeking the homogeneity of the lots. The objective was to evaluate the variation of Body Weight (PC), ECC and Thickness of Bacon (ET) up to 107 days of gestation. For this purpose, CP and ECC measurements were performed every two weeks. ET was evaluated each 21 days. The data were submitted to descriptive and regression analysis over time. It was observed at the end of gestation that the group housed in the pens with an electronic feeding system showed less unevenness in relation to the PC and greater control of ECC. These results, added to the reduction of hierarchical disputes and for food competition, are important for maintaining the welfare of these females. Thus, automated feeding system proved to be important allies on the creation of pregnant swine matrices in collective pens.
长白猪和大型杂交猪雌性在妊娠期被安置在集体围栏中,并接受不同的喂养系统。在其中一个外壳中使用了手动进给系统,进给直接在地板上提供。另一个是在笼子里安装了电子喂食系统,可以免费使用Gestal 3G。两组,每组由三个矩阵组成,由外壳隔开。雌性根据其身体状况评分(ECC)和出生顺序进行分配,以寻求批次的同质性。目的是评估妊娠107天内体重(PC)、ECC和培根厚度(ET)的变化。为此,每两周进行一次CP和ECC测量。每21天对ET进行一次评估。数据经过一段时间的描述性和回归分析。在妊娠末期观察到,被安置在具有电子喂养系统的围栏中的组显示出与PC相比更少的不均匀性和对ECC的更大控制。这些结果,加上减少了等级纠纷和粮食竞争,对维护这些女性的福利很重要。因此,自动化饲养系统被证明是在集体围栏中创建怀孕猪基质的重要盟友。
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引用次数: 0
COMPARAÇÃO ENTRE PROTOCOLOS COM DESMAME TEMPORÁRIO VERSUS GONADOTROFINA CORIÔNICA EQUINA APÓS TRATAMENTO COM PROGESTERONA INTRAVAGINAL VISANDO A INSEMINAÇÃO ARTIFICIAL EM TEMPO FIXO EM VACAS BOS TAURUS INDICUS 牛临时断奶与马绒毛膜促性腺激素在阴道内孕酮固定时间人工授精后的方案比较
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.75408
André Luiz Bastos de Souza, M. S. Segui, L. E. Kozicki, V. Pedrosa, Rafaela Talini, R. R. Weiss, Natalia Santana Siqueira Lara
The aim of the study was to compare the reproductive efficiency of two protocols with temporary weaning (TW) of calves and one using equine chorionic gonadotropin (eCG), the synchronization of estrus and ovulation in timed artificial insemination (TAI) protocol. A total of 534 multiparous cows were distributed into three groups: temporary weaning on day 8 of the protocol group (TW8G; n = 225); temporary weaning on day 9 group (TW9G; n = 139) and equine chorionic gonadotropin group (eCGG, n = 170). TW8G received on day zero (d0) an intravaginal device with progesterone (P4; 0.558g) + estradiol benzoate (EB; 2mg, im). On d8, the device was removed with P4 + PGF (0.075mg, im) + estradiol cypionate (EC 0.5 mg, im) + removal of the calf for 48 hours. On d10, TAI was performed, and the calves returned to their mothers. The TW9G was subjected to the same protocol as the TW8G, except for the removal of the device with the P4, which occurred on the 9th, and on the d11, TAI was performed, and the calves returned to their mothers. eCGG was subjected to the same protocol as TW8G, except that on d8 it received eCG (300IU, im), on d9 estradiol benzoate and TAI on d10. The pregnancy diagnosis was made change for done at d45. The non-pregnant cows (post TAI) were re-inseminated under the observation for estrus observation (conventional AI). The remaining empty cows from conventional AI remained with the bull for another 45 days. PR after TAI was 47.1 (TW8G), 40.2 (TW9G) and 51.7% (GeCG), while PR after conventional AI was 19.3%; 31.3% and 26.8%. PR at the end of the breeding season resulted in 95.1, 90.6, and 92.3% respectively for TW8G, TW9G and eCGG. It was concluded that there was no difference in reproductive efficiency in the timed artificial insemination between the applied protocols; the use of TW or eCG can be implemented in the breeding season of beef cows.
本研究的目的是比较犊牛临时断奶(TW)和马绒毛膜促性腺激素(eCG)两种方案的生殖效率,以及定时人工授精(TAI)方案的发情和排卵同步。试验将534头产牛分为3组:方案组(TW8G)第8天临时断奶;N = 225);第9天临时断奶组(TW9G;n = 139)和马绒毛膜促性腺激素组(eCGG, n = 170)。TW8G在第0天(d0)接受含黄体酮的阴道内装置(P4;0.558g) +雌二醇苯甲酸酯(EB;2毫克,im)。在第8天,用P4 + PGF (0.075mg, im) +雌二醇(EC 0.5 mg, im) +去除小牛48小时。10日,进行了TAI,幼崽回到了它们的母亲身边。TW9G接受与TW8G相同的治疗方案,除了P4装置的移除(发生在9日)和d11,进行TAI,小牛回到他们的母亲身边。eCGG的治疗方案与TW8G相同,但在第8天接受心电图(300IU, im),第9天接受苯甲酸雌二醇治疗,第10天接受TAI治疗。妊娠诊断更改为在第45天完成。对未怀孕的母牛(TAI后)在观察发情(常规AI)的情况下再次人工授精。传统人工智能中剩下的空牛和公牛一起待了45天。TAI后的PR分别为47.1 (TW8G)、40.2 (TW9G)和51.7% (GeCG),而常规AI后的PR为19.3%;31.3%和26.8%。繁殖季末,TW8G、TW9G和eCGG的PR分别为95.1、90.6和92.3%。结果表明,不同应用方案在定时人工授精的生殖效率上无显著差异;在肉牛的繁殖期可实施TW或eCG。
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引用次数: 0
PREVALÊNCIA DE ANTICORPOS ANTI-VÍRUS DA LEUCOSE BOVINA EM FÊMEAS DE BOVINOS LEITEIROS DE TOLEDO, PARANÁ, BRASIL 巴西托莱多奶牛抗牛白血病病毒抗体的流行情况,parana
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.78396
Anderson Demarchi Peron, Cristian André Bertuzzi, Douglas Adriano Webber, V. Holsbach, D. L. Zulpo
The enzootic bovine leukosis is a viral disease caused by the bovine leukemia virus (BLV), an oncogenic retrovirus. The disease is widespread in herds, and dairy cattle, due to the intensive management, are the most affected. The growing importance of the international cattle trade, semen, and embryos has required the sanitary certification for the BLV, and the economic impact of the enzootic bovine leukosis is mainly due to the reduction of the milk production. The aim of this study was to determine the prevalence of antibody antiBLV in dairy cattle from Toledo municipality. Fifty-eight rural properties were visited, and blood samples were collected from 390 dairy cattle. The agar gel immunodiffusion (AGID) was used to detect the antibodies against the viral agent. Of the samples analyzed, 34.4% (134/390) showed antibodies against the bovine leukosis. Positive animals were distributed in 63.9% (37/58) of the visited properties. The viral infection is widespread in dairy cattle from Toledo. Therefore, there is a need for adequate control and prevention measures for enzootic bovine leukosis. Serological tests and a constant veterinary follow-up, aiming at the gradual sanitary control of the herds, are a good tool for the control and/or eradication of the disease.
地方源性牛白细胞病是由一种致癌逆转录病毒——牛白血病病毒(BLV)引起的一种病毒性疾病。这种疾病在牛群中广泛传播,奶牛由于管理密集,受到的影响最大。国际牛贸易、精液和胚胎的重要性日益增加,要求获得BLV的卫生认证,而地方病牛白细胞病的经济影响主要是由于牛奶产量的减少。本研究的目的是确定抗体抗BLV在托莱多市奶牛中的流行率。访问了58处农村财产,并从390头奶牛身上采集了血液样本。使用琼脂凝胶免疫扩散法(AGID)检测针对病毒制剂的抗体。在分析的样本中,34.4%(134/390)显示出抗牛白细胞病的抗体。阳性动物分布在63.9%(37/58)的受访房产中。病毒感染在托莱多的奶牛中广泛存在。因此,有必要采取适当的控制和预防措施来控制和预防地方病牛白细胞病。血清学检测和持续的兽医随访,旨在逐步对牛群进行卫生控制,是控制和/或根除疾病的好工具。
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引用次数: 0
MOLECULAR DETECTION AND SEROPREVALENCE OF Ehrlichia sp. IN DAIRY CATTLE FROM BRAZIL’S WESTERN AMAZON REGION 巴西西亚马逊地区奶牛埃利希体的分子检测及血清阳性率
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.74784
Rute Witter, A. L. T. Melo, T. A. Pacheco, V. Dutra, L. Nakazato, C. S. Chitarra, A. C. S. Oliveira, D. M. Aguiar, R. Pacheco
Ehrlichia minasensis, a novel species of Ehrlichia that is closely related to E. canis, is known to infect cattle and deer in Canada. This rickettsial bacterium was isolated from Rhipicephalus (Boophilus) microplus ticks and from dairy and beef cattle in southeast and midwestern Brazil, respectively. The purpose of this study was to determine the seroprevalence and perform molecular detection of Ehrlichia sp. in dairy cattle in the northern region of Brazil. The study was conducted in the municipality of Ji-Paraná, located in the state of Rondônia in the western Brazilian Amazon region. Blood and serum samples were obtained between the dates September 2012 and November 2013 from dairy cows (≥ 24 months old) in 64 farms. The blood samples were subjected to polymerase chain reaction (PCR) to amplify a fragment of the Ehrlichia sp. dsb gene, and the levels of Ehrlichia sp. antibodies were measured by the immunofluorescence assay (IFA). A total of 15 of 610 (2.45%; 95% CI: 1.04-3.86%) blood samples tested positive for ehrlichial infection based on the detection of the Ehrlichia sp. dsb gene. Sequencing of PCR amplicons from samples confirmed that the amplified partial dsb gene (~295 base pairs) sequence represented E. minasensis. Anti-Ehrlichia sp. antibodies were detected in 178 cows (53.96%; 95% CI: 46.63–61.29%). Endpoint titers ranged from 40 to 5,120. However, positive results derived from this assay should be interpreted with caution. Among the variables analyzed using IFA, the number of cows aged 24 months or greater was statistically significant (p = 0.0103), and hers of approximately 51-100 cows were more likely to be infected with Ehrlichia sp. Although the animals do not show clinical disease, the chronic character of the infection can lead to decrease in productivity.
minasensis是一种与E. canis密切相关的新型埃利希体,已知在加拿大感染牛和鹿。该立克次体细菌分别从巴西东南部和中西部的微型蜱和乳牛和肉牛中分离得到。本研究的目的是确定埃利希体在巴西北部地区奶牛中的血清流行率并进行分子检测。这项研究是在巴西西部亚马逊地区Rondônia州的ji - paranar市进行的。在2012年9月至2013年11月期间,对64个农场的奶牛(≥24月龄)采集了血液和血清样本。采用聚合酶链反应(PCR)扩增埃利希体dsb基因片段,采用免疫荧光法(IFA)检测埃利希体抗体水平。610人中有15人(2.45%;95% CI: 1.04-3.86%),根据埃利希氏杆菌dsb基因的检测,血液样本检测为埃利希氏感染阳性。PCR扩增子测序证实,扩增的部分dsb基因(~295个碱基对)序列为米纳沙伊蚊。检出抗埃利希体抗体178头(53.96%);95% ci: 46.63-61.29%)。终点滴度范围从40到5120。然而,从该试验中得出的阳性结果应谨慎解释。在IFA分析的变量中,24月龄及以上的奶牛数量具有统计学意义(p = 0.0103),大约51-100头奶牛更容易感染埃利希体。尽管动物没有表现出临床疾病,但感染的慢性特征会导致生产力下降。
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引用次数: 0
ANÁLISE COPROPARASITOLÓGICA DE GAMBÁS-DE-ORELHA-BRANCA PERTENCENTES A ÁREAS URBANAS E RURAIS DO MUNICÍPIO DE CANOINHAS, NORTE DE SANTA CATARINA 圣卡塔琳娜北部卡诺尼亚斯市城市和农村地区白耳负鼠的共寄生虫学分析
Q4 Veterinary Pub Date : 2021-06-29 DOI: 10.5380/AVS.V26I2.76985
Juliano Biolchi, Giane Helenita Pontarolo, Daniele de Cassia Karvat, D. Pedrassani
The opossums are synanthropic mammals belonging to the genus Didelphis. They have a generalized diet, and this behavior exposes them to the chance of infection by several parasitic agents. Their close relationship with the human makes them act as disseminators of pathogens. The objective of the study was to evaluate the diversity of endoparasites infecting D. albiventris sampled in Canoinhas, Northern Santa Catarina. Thirty-eigth fecal samples were macro and microscopically analyzed, and of these 89.47% showed positivity for at least one endoparasite, 89.47% (34/38) for helminths and 60.52% (23/38) for protozoa. Nematode eggs of the order Strongylida and the genera Cruzia, Trichuris, Aspidodera, Physaloptera, Capillaria and Strongyloides were observed, as well as trematode eggs and oocysts of protozoa. In 38.3% of the animals four parasite types were detected, showing high occurrence of endoparasites and that multiparasitism is frequent in this species.
负鼠是一种共生哺乳动物,属于负鼠属。他们有一个普遍的饮食,这种行为使他们有机会感染几种寄生虫。它们与人类的密切关系使它们成为病原体的传播者。本研究的目的是评估在圣卡塔琳娜州北部Canoinhas采样的感染白腹锥虫的内寄生虫的多样性。对第30份粪便样本进行了宏观和微观分析,其中89.47%的样本显示至少一种内寄生虫阳性,89.47%(34/38)的样本显示蠕虫阳性,60.52%(23/38)的样本表示原生动物阳性。观察到了坚线虫目、克鲁齐亚属、鞭虫属、蜘蛛目、植翅目、毛细管属和坚线虫属的线虫卵,以及原生动物的吸虫卵和卵囊。在38.3%的动物中,检测到四种寄生虫类型,表明内寄生虫病的发生率很高,并且该物种中多寄生现象很常见。
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引用次数: 1
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Archives of Veterinary Science
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