Nássarah Jabur Lot Rodrigues, S. Manzini, Natalie Newirt Oliveira, M. Z. E. Gava, Lenise Garbelotti Gonçalves, I. N. Hirota, A. P. Ferreira, Mariana De Sessa, Mayara Chagas Ferreira, Luis Felipe Fernandes Reiter, C. Brandão, H. Langoni, B. Menozzi
{"title":"SEROLOGICAL ANTIBODY RESPONSE OF THE CLASSES IgM AND IgG ANTI-Toxoplasma gondii IN DOGS WITH OCULAR ALTERATIONS.","authors":"Nássarah Jabur Lot Rodrigues, S. Manzini, Natalie Newirt Oliveira, M. Z. E. Gava, Lenise Garbelotti Gonçalves, I. N. Hirota, A. P. Ferreira, Mariana De Sessa, Mayara Chagas Ferreira, Luis Felipe Fernandes Reiter, C. Brandão, H. Langoni, B. Menozzi","doi":"10.5380/avs.v26i4.81431","DOIUrl":"https://doi.org/10.5380/avs.v26i4.81431","url":null,"abstract":"","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46989148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Ambrosini, Melissa Garcia, Victória Gariba e Silva, A. Rossa, T. L. Alves, R. Sousa, V. Albernaz, M. G. Sousa, S. T. O. Stedile
Lymphoma is a hematopoietic neoplasia which occurs due to the proliferation of malignant lymphocytes. It can be present in different forms, including alimentary, mediastinal, multicentric and extranodal. In both cases described in this report, signs consistent with chronic kidney disease were present. An ultrasound-guided fine-needle aspiration was performed and suggested a lymphocyte neoplastic process. Both cats received prednisolone and life support treatment, with mild improvement. One cat was euthanatized and the other died spontaneously, five and seven days after onset of treatment, respectively. The kidneys were enlarged, whitish, irregular, with a firm mass in the cortex. Histopathology confirmed that the lymphoma was restricted to the kidneys.
{"title":"PRIMARY RENAL LYMPHOMA IN TWO CATS: CASE REPORT","authors":"F. Ambrosini, Melissa Garcia, Victória Gariba e Silva, A. Rossa, T. L. Alves, R. Sousa, V. Albernaz, M. G. Sousa, S. T. O. Stedile","doi":"10.5380/avs.v26i4.72701","DOIUrl":"https://doi.org/10.5380/avs.v26i4.72701","url":null,"abstract":"Lymphoma is a hematopoietic neoplasia which occurs due to the proliferation of malignant lymphocytes. It can be present in different forms, including alimentary, mediastinal, multicentric and extranodal. In both cases described in this report, signs consistent with chronic kidney disease were present. An ultrasound-guided fine-needle aspiration was performed and suggested a lymphocyte neoplastic process. Both cats received prednisolone and life support treatment, with mild improvement. One cat was euthanatized and the other died spontaneously, five and seven days after onset of treatment, respectively. The kidneys were enlarged, whitish, irregular, with a firm mass in the cortex. Histopathology confirmed that the lymphoma was restricted to the kidneys.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48451415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paula Carvalho Leal, A. Massuquetto, Marley Conceição dos Santos, Luis Daniel Giusti Bruno, E. L. Krabbe, Ananda Portella Felix, A. Maiorka
The objective of this study was to analyze the physical-chemical characteristics of corn lots with different ratios of damaged to intact grains, considering the interaction between them. Recently harvested corn grains were selected, and grains attacked by fungi (fermented and moldy) and intact grains were separated to establish the following grain mixtures (treatments): 1 – 0% fermented/moldy and 100% intact grains; 2 - 10% fermented/moldy and 90% intact grains; 3 - 20% fermented/moldy and 80% intact grains; 4 - 30% fermented/moldy and 70% intact grains; and 5 - 40% fermented/moldy and 60% intact grains. Samples from each treatment were analyzed for dry matter (DM), ash (A), crude protein (CP), crude fiber (CF), ether extract (EE), and gross energy (GE) contents, water activity (aw), density, and hardness. Chemical composition and density data were submitted to analysis of regression, hardness values were compared by Tukey test (P<0.05), and Pearson’s correlations (P<0.05) between chemical and physical parameters were calculated. Increasing ratios of moldy corn relative to intact corn reduced DM, CF, EE, and GE contents, increased A, CP, Wa values, and reduced the density of the lot. Moldy grains presented lower hardness values compared with intact corn. This study shows that fungus damage affects corn grain physical-chemical properties.
{"title":"Fungus Damage Effect on Physical-Chemical Characteristics of Corn Grains","authors":"Paula Carvalho Leal, A. Massuquetto, Marley Conceição dos Santos, Luis Daniel Giusti Bruno, E. L. Krabbe, Ananda Portella Felix, A. Maiorka","doi":"10.5380/avs.v26i4.77519","DOIUrl":"https://doi.org/10.5380/avs.v26i4.77519","url":null,"abstract":"The objective of this study was to analyze the physical-chemical characteristics of corn lots with different ratios of damaged to intact grains, considering the interaction between them. Recently harvested corn grains were selected, and grains attacked by fungi (fermented and moldy) and intact grains were separated to establish the following grain mixtures (treatments): 1 – 0% fermented/moldy and 100% intact grains; 2 - 10% fermented/moldy and 90% intact grains; 3 - 20% fermented/moldy and 80% intact grains; 4 - 30% fermented/moldy and 70% intact grains; and 5 - 40% fermented/moldy and 60% intact grains. Samples from each treatment were analyzed for dry matter (DM), ash (A), crude protein (CP), crude fiber (CF), ether extract (EE), and gross energy (GE) contents, water activity (aw), density, and hardness. Chemical composition and density data were submitted to analysis of regression, hardness values were compared by Tukey test (P<0.05), and Pearson’s correlations (P<0.05) between chemical and physical parameters were calculated. Increasing ratios of moldy corn relative to intact corn reduced DM, CF, EE, and GE contents, increased A, CP, Wa values, and reduced the density of the lot. Moldy grains presented lower hardness values compared with intact corn. This study shows that fungus damage affects corn grain physical-chemical properties.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44332828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edson Mendes da Silva Júnior, Silmara Rossi, Roberta Ramblas Zamana, Marco Aurélio Gattamorta, Augusto Carlos da Bôaviagem Freire, Rafael Ângelo Revorêdo, Daniel Solon Dias de Farias, Carlos Sacristán, Aline da Costa Bomfim, E. R. Matushima, Flávio José de Lima Silva, Simone Almeida Gavilan
As tartarugas-verdes (Chelonia mydas) são afetadas pela fibropapilomatose (FP), doença que pode ser uma ameaça para elas. O trabalho teve como objetivo descrever as características histopatológicas dos tumores sugestivos de FP e pesquisar a presença de DNA de Chelonid alphaherpesvirus 5 (ChHV5) em lesões cutâneas de 26 tartarugas verdes encalhadas na Bacia Potiguar (nordeste do Brasil). As tartarugas verdes foram encontradas durante o monitoramento diário, e os tumores de FP foram coletados por biópsia ou durante necropsia, quantificados e analisados de acordo com distribuição anatômica, tamanho e Escore de Fibropapilomatose do Atlântico Sudoeste (FPSSWA). Vinte e seis cortes histológicos foram examinados, e 26 amostras fixadas em formalina e embebidas em parafina foram analisadas por reação em cadeia da polimerase (PCR) para detectar o DNA do ChHV5. Um total de 292 tumores foi analisado (1-42 por indivíduo), sendo a maioria com tamanho de 1-4 cm (74%; 218/292 tumores) e presentes nos membros anteriores (48,6%; 142/292 tumores). De acordo com o FPSSWA, a maioria das tartarugas verdes estava afetada de forma leve (53,8%; 14/26). O exame histopatológico revelou características previamente descritas na literatura (projeções papilares da camada epitelial, hiperceratose, hiperplasia da camada basal, fibroblastos em proliferação bem diferenciados) classificando os tumores como papilomas ou fibropapilomas. Com relação aos resultados de PCR, 79,9% das amostras de tumor testadas foram positivas para ChHV5 e as sequências foram obtidas em todas as amostras amplificadas. Nossas sequências UL30 são idênticas a várias sequências descritas em tartarugas marinhas do Brasil, enquanto as sequências UL18 e UL27 são mais semelhantes as de tartarugas verdes na Flórida. Nosso estudo relata detecção e caracterização molecular do ChHV5 em tartarugas verdes encalhadas na Bacia Potiguar, importante área de alimentação com alta frequência de ocorrência de FP.
{"title":"CHELONID ALPHAHERPESVIRUS 5 EM TUMORES CUTÂNEOS DE TARTARUGAS VERDES (CHELONIA MYDAS) ENCALHADAS NO NORDESTE DO BRASIL","authors":"Edson Mendes da Silva Júnior, Silmara Rossi, Roberta Ramblas Zamana, Marco Aurélio Gattamorta, Augusto Carlos da Bôaviagem Freire, Rafael Ângelo Revorêdo, Daniel Solon Dias de Farias, Carlos Sacristán, Aline da Costa Bomfim, E. R. Matushima, Flávio José de Lima Silva, Simone Almeida Gavilan","doi":"10.5380/avs.v26i4.81329","DOIUrl":"https://doi.org/10.5380/avs.v26i4.81329","url":null,"abstract":"As tartarugas-verdes (Chelonia mydas) são afetadas pela fibropapilomatose (FP), doença que pode ser uma ameaça para elas. O trabalho teve como objetivo descrever as características histopatológicas dos tumores sugestivos de FP e pesquisar a presença de DNA de Chelonid alphaherpesvirus 5 (ChHV5) em lesões cutâneas de 26 tartarugas verdes encalhadas na Bacia Potiguar (nordeste do Brasil). As tartarugas verdes foram encontradas durante o monitoramento diário, e os tumores de FP foram coletados por biópsia ou durante necropsia, quantificados e analisados de acordo com distribuição anatômica, tamanho e Escore de Fibropapilomatose do Atlântico Sudoeste (FPSSWA). Vinte e seis cortes histológicos foram examinados, e 26 amostras fixadas em formalina e embebidas em parafina foram analisadas por reação em cadeia da polimerase (PCR) para detectar o DNA do ChHV5. Um total de 292 tumores foi analisado (1-42 por indivíduo), sendo a maioria com tamanho de 1-4 cm (74%; 218/292 tumores) e presentes nos membros anteriores (48,6%; 142/292 tumores). De acordo com o FPSSWA, a maioria das tartarugas verdes estava afetada de forma leve (53,8%; 14/26). O exame histopatológico revelou características previamente descritas na literatura (projeções papilares da camada epitelial, hiperceratose, hiperplasia da camada basal, fibroblastos em proliferação bem diferenciados) classificando os tumores como papilomas ou fibropapilomas. Com relação aos resultados de PCR, 79,9% das amostras de tumor testadas foram positivas para ChHV5 e as sequências foram obtidas em todas as amostras amplificadas. Nossas sequências UL30 são idênticas a várias sequências descritas em tartarugas marinhas do Brasil, enquanto as sequências UL18 e UL27 são mais semelhantes as de tartarugas verdes na Flórida. Nosso estudo relata detecção e caracterização molecular do ChHV5 em tartarugas verdes encalhadas na Bacia Potiguar, importante área de alimentação com alta frequência de ocorrência de FP.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43236956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. S. Araújo, Flávia Vitória Pereira de Moura, Luís Evêncio Da Luz
{"title":"AVALIAÇÃO DA QUALIDADE HIGIÊNICO-SANITÁRIA E FÍSICO-ESTRUTURAL DE AÇOUGUES EM MUNICÍPIOS DO VALE DO GUARIBAS, NO ESTADO DO PIAUÍ","authors":"D. S. Araújo, Flávia Vitória Pereira de Moura, Luís Evêncio Da Luz","doi":"10.5380/avs.v26i4.82485","DOIUrl":"https://doi.org/10.5380/avs.v26i4.82485","url":null,"abstract":"","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45716600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. B. Barros, Daianny Barbosa Guimarães, Alina Viana Dias, A. A. Moura, Lina Raquel Santos Araújo, R. Toniolli
{"title":"PROTEÍNAS DO PLASMA SEMINAL ASSOCIADAS À CONGELABILIDADE DO SÊMEN SUÍNO","authors":"T. B. Barros, Daianny Barbosa Guimarães, Alina Viana Dias, A. A. Moura, Lina Raquel Santos Araújo, R. Toniolli","doi":"10.5380/avs.v26i4.79126","DOIUrl":"https://doi.org/10.5380/avs.v26i4.79126","url":null,"abstract":"","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48165347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. R. Ribeiro, A. L. Andrade, Ana Letícia Groszewicz De Souza, A. P. Oriá, Fabiano Montiani-Ferreira, P. D. Galera, A. V. Safatle, C. Brandão, Fábio Luiz da Cunha Brito
ANAIS DO XVII CONGRESSO BRASILEIRO DE OFTALMOLOGIA VETERINARIA REALIZADO EM FOZ DE IGUACU-PR, NOS DIAS 17, 18 E 19 DE NOVEMBRO DE 2021, NO BOURBON CATARATAS DO IGUACU RESORT E ORGANIZADO PELO COLEGIO BRASILEIRO DE OFTALMOLOGISTAS VETERINARIOS (CBOV).
2021年11月17日、18日和19日,由巴西兽医学院(CBOV)组织的BOURBON CATRATAS DO IGUACUResort在FOZ DE IGUACU-PR举行的第十七届巴西兽医学大会分析。
{"title":"ANAIS DO XVII CONGRESSO BRASILEIRO DE OFTALMOLOGIA VETERINÁRIA","authors":"C. R. Ribeiro, A. L. Andrade, Ana Letícia Groszewicz De Souza, A. P. Oriá, Fabiano Montiani-Ferreira, P. D. Galera, A. V. Safatle, C. Brandão, Fábio Luiz da Cunha Brito","doi":"10.5380/AVS.V26I4.83585","DOIUrl":"https://doi.org/10.5380/AVS.V26I4.83585","url":null,"abstract":"ANAIS DO XVII CONGRESSO BRASILEIRO DE OFTALMOLOGIA VETERINARIA REALIZADO EM FOZ DE IGUACU-PR, NOS DIAS 17, 18 E 19 DE NOVEMBRO DE 2021, NO BOURBON CATARATAS DO IGUACU RESORT E ORGANIZADO PELO COLEGIO BRASILEIRO DE OFTALMOLOGISTAS VETERINARIOS (CBOV).","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48133112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anna Paula Balesdent Barreira, Letícia Rodrigues Leitão Andrade, D. Oliveira, I. Rocha, C.F.P.M. Carvalho, S. Thomé
This study aims to identify epidemiological factors that could predispose the companion animals of Seropédica and other cities in Rio de Janeiro to the development of breast cancer, as well as to analyze the veterinarian's contribution inpreventing the disease. For this, questionnaires answered by tutors in an awareness campaign about breast cancer in bitches and cats were evaluated to identifypredisposing factors and risk to the disease, access to veterinary care and guidance on the topic. 1,143 questionnaires were analyzed, which revealed the presence of highly predisposing factors such as the predominance of females, of the canine species and of small canine breeds. The low average age of the population studied was the only factor that indicated a low predisposition. As for the risk factors analyzed, the predominance of non-castrated animals revealed a high risk of developing the disease, while the low occurrence of pseudocyesis and the use of contraceptives were not impact factors in this population. In addition, only 29.3% of tutors who took their animals for veterinary care were instructed on the disease and / or preventive methods such as castration. It is concluded that the analyzed population has a high predisposition to the development of breast neoplasms, which should intensify the training of the veterinarian to include preventive guidance during consultations, expand educational campaigns on the subject and castrations in the region of Seropédica and surroundings.
{"title":"AVALIAÇÃO DA PREDISPOSIÇÃO DE CADELAS E GATAS PARA A NEOPLASIA MAMÁRIA EM MUNICÍPIOS DO RIO DE JANEIRO","authors":"Anna Paula Balesdent Barreira, Letícia Rodrigues Leitão Andrade, D. Oliveira, I. Rocha, C.F.P.M. Carvalho, S. Thomé","doi":"10.5380/AVS.V26I3.81077","DOIUrl":"https://doi.org/10.5380/AVS.V26I3.81077","url":null,"abstract":"This study aims to identify epidemiological factors that could predispose the companion animals of Seropédica and other cities in Rio de Janeiro to the development of breast cancer, as well as to analyze the veterinarian's contribution inpreventing the disease. For this, questionnaires answered by tutors in an awareness campaign about breast cancer in bitches and cats were evaluated to identifypredisposing factors and risk to the disease, access to veterinary care and guidance on the topic. 1,143 questionnaires were analyzed, which revealed the presence of highly predisposing factors such as the predominance of females, of the canine species and of small canine breeds. The low average age of the population studied was the only factor that indicated a low predisposition. As for the risk factors analyzed, the predominance of non-castrated animals revealed a high risk of developing the disease, while the low occurrence of pseudocyesis and the use of contraceptives were not impact factors in this population. In addition, only 29.3% of tutors who took their animals for veterinary care were instructed on the disease and / or preventive methods such as castration. It is concluded that the analyzed population has a high predisposition to the development of breast neoplasms, which should intensify the training of the veterinarian to include preventive guidance during consultations, expand educational campaigns on the subject and castrations in the region of Seropédica and surroundings.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48670340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rodolfo Luiz Godoy do Amaral, Neurisvan Ramos Guerra, Ivanise Maria de Santana, Yanise Chicoy-Ramirez, Amilcar Arenal-Cruz, M. M. D. Lima, L. C. Alves, M. Molento, Maria da Assunção Duarte Faustino
Sheep and goats suffer heavy losses due to the high prevalence of gastrointestinal nematodes, mainly from the nematode Haemonchus contortus. The parasite causes high morbidity and mortality, even though, the indiscriminate use of chemicals to control H. contortus has favored the selection of multidrug resistance parasites. Molecular diagnosis of resistance to the benzimidazole (BZs) group is important to indicate substitute strategies of control. The aim of this study was to determine the presence of the mutation at position F200Y of the beta-tubulin gene in seven populations of H. contortus from sheep and goats’ farms from the state of Pernambuco, Brazil. Twenty adult H. contortus male worms were recovered for DNAextraction from naturally infected animals, from farms from Recife, Sairé, Camocim deSão Félix, Gameleira, Moreno, Bonito, and Serra Talhada. The primers forward: 5` AAT GCT TCC ACC CTT GTC CAT C 3’, and reverse: 5’ CAA CAA CGG GCA TGA AGA AG 3’ were used to search for the main codon (F200Y) of the beta-tubulin gene to determine BZ resistance. The NC1/ITS-2 forward: 5’ ACG TCT GGT TCA GGG GTT TT 3’ and reverse: 5’ TTA TCT GTT TTT CCT GCC CT 3’ primers were used for confirming H. contortus species. A questionnaire was applied to obtain information on management practices, including the frequency of BZs use. The sequences from the NC1/ITS-2 confirmed the presence of H. contortus from all samples. The F200Y mutation responsible for BZ resistance (TTC/TAC) in H. contortus was found only in the samples from Sairé, as the TTC codon varied from 55.6 to 85.7%. In relation to the questionnaire, producers revealed the absence of quarantine for newly arrived animals; high drug treatment frequency (60-days interval); treatment of the whole flock; the visual estimation of the weight of the animals for dose determination; and high drug rotation. It was also mentioned the lack of veterinary assistance with no use of fecal exams. Even though, all these factors may be essential to increase parasite selection pressure, it was not possible to determine which of those showed a higher risk due to the high number of variables and the small number of participants. This is without any doubt one of the great difficulties to correlate phenotypic drug efficacy data (ex. FECRT) and genotypic-related (ex. PCR) resistance markers in helminths. We have included some risk factors reported by the farmers from Pernambuco and their influences in the process of drug selection, as well as a few strategies to mitigate this situation. The present report constitutes the first record of a molecular diagnosis of the mutation F200Y that confers resistance to the BZ group in ruminants in the state of Pernambuco, Brazil.
绵羊和山羊由于胃肠道线虫的高流行率而遭受重大损失,这些线虫主要来自扭曲血蜱。这种寄生虫会导致高发病率和高死亡率,尽管滥用化学物质来控制扭曲线虫有利于选择耐多药寄生虫。对苯并咪唑(BZs)组耐药性的分子诊断对于指示控制的替代策略是重要的。本研究的目的是确定来自巴西伯南布哥州绵羊和山羊农场的七个扭曲H.群体中β-微管蛋白基因F200Y位置是否存在突变。从Recife、Sairé、Camocim de São Félix、Gameleira、Moreno、Bonito和Serra Talhada的农场中,从自然感染的动物身上回收了20只成年扭曲H.雄性蠕虫进行DNA提取。用正向引物5’AAT-GCT-TCC ACC CTT-GTC CAT C3’和反向引物5’CAA-CAA CGG-GCA-TGA-AGA AG 3’寻找β-微管蛋白基因的主密码子(F200Y),测定BZ抗性。采用NC1/ITS-2正向引物:5’ACG TCT GGT TCA GGG GTT TT 3’和反向引物:5‘TTA TCT GTT TTT CCT GCC CT 3’对扭线虫进行物种鉴定。采用问卷调查法获得管理实践方面的信息,包括BZ的使用频率。来自NC1/ITS-2的序列证实了来自所有样品的扭曲H。扭曲线虫BZ抗性(TTC/TAC)的F200Y突变仅在Sairé的样本中发现,因为TTC密码子的变化范围为55.6%至85.7%。在问卷调查中,生产商透露对新来的动物没有隔离;药物治疗频率高(间隔60天);整群羊的处理;用于剂量测定的动物重量的视觉估计;以及高药物轮换。它还提到缺乏兽医援助,没有使用粪便检查。尽管所有这些因素可能对增加寄生虫选择压力至关重要,但由于变量数量多,参与者人数少,因此无法确定哪些因素的风险更高。毫无疑问,这是将蠕虫的表型药物疗效数据(如FECRT)和基因型相关(如PCR)耐药性标记物关联起来的巨大困难之一。我们纳入了伯南布哥农民报告的一些风险因素及其在药物选择过程中的影响,以及缓解这种情况的一些策略。本报告构成了巴西伯南布哥州反刍动物对BZ组产生耐药性的突变F200Y的分子诊断的第一个记录。
{"title":"DIAGNÓSTICO MOLECULAR DA RESISTÊNCIA DE HAEMONCHUS CONTORTUS AO GRUPO DOS BENZIMIDAZÓIS EM OVINOS E CAPRINOS NO ESTADO DE PERNAMBUCO, BRASIL","authors":"Rodolfo Luiz Godoy do Amaral, Neurisvan Ramos Guerra, Ivanise Maria de Santana, Yanise Chicoy-Ramirez, Amilcar Arenal-Cruz, M. M. D. Lima, L. C. Alves, M. Molento, Maria da Assunção Duarte Faustino","doi":"10.5380/AVS.V26I3.79112","DOIUrl":"https://doi.org/10.5380/AVS.V26I3.79112","url":null,"abstract":"Sheep and goats suffer heavy losses due to the high prevalence of gastrointestinal nematodes, mainly from the nematode Haemonchus contortus. The parasite causes high morbidity and mortality, even though, the indiscriminate use of chemicals to control H. contortus has favored the selection of multidrug resistance parasites. Molecular diagnosis of resistance to the benzimidazole (BZs) group is important to indicate substitute strategies of control. The aim of this study was to determine the presence of the mutation at position F200Y of the beta-tubulin gene in seven populations of H. contortus from sheep and goats’ farms from the state of Pernambuco, Brazil. Twenty adult H. contortus male worms were recovered for DNAextraction from naturally infected animals, from farms from Recife, Sairé, Camocim deSão Félix, Gameleira, Moreno, Bonito, and Serra Talhada. The primers forward: 5` AAT GCT TCC ACC CTT GTC CAT C 3’, and reverse: 5’ CAA CAA CGG GCA TGA AGA AG 3’ were used to search for the main codon (F200Y) of the beta-tubulin gene to determine BZ resistance. The NC1/ITS-2 forward: 5’ ACG TCT GGT TCA GGG GTT TT 3’ and reverse: 5’ TTA TCT GTT TTT CCT GCC CT 3’ primers were used for confirming H. contortus species. A questionnaire was applied to obtain information on management practices, including the frequency of BZs use. The sequences from the NC1/ITS-2 confirmed the presence of H. contortus from all samples. The F200Y mutation responsible for BZ resistance (TTC/TAC) in H. contortus was found only in the samples from Sairé, as the TTC codon varied from 55.6 to 85.7%. In relation to the questionnaire, producers revealed the absence of quarantine for newly arrived animals; high drug treatment frequency (60-days interval); treatment of the whole flock; the visual estimation of the weight of the animals for dose determination; and high drug rotation. It was also mentioned the lack of veterinary assistance with no use of fecal exams. Even though, all these factors may be essential to increase parasite selection pressure, it was not possible to determine which of those showed a higher risk due to the high number of variables and the small number of participants. This is without any doubt one of the great difficulties to correlate phenotypic drug efficacy data (ex. FECRT) and genotypic-related (ex. PCR) resistance markers in helminths. We have included some risk factors reported by the farmers from Pernambuco and their influences in the process of drug selection, as well as a few strategies to mitigate this situation. The present report constitutes the first record of a molecular diagnosis of the mutation F200Y that confers resistance to the BZ group in ruminants in the state of Pernambuco, Brazil.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46134583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marcella Dall Agnol Leite, F. C. Mota, A. E. Alves, G. M. Nogueira, M. M. Mantovani, Matheus Franco da Veiga Teixeira
Fractures in dogs are one of the great causes of care in small animal surgical clinic. The Interlocking Nail (IN) is a widely used implant, they resist flexion, torsion and axial compression forces. The present study aims to evaluate the time of consolidation, formation of bone callus, and possible complications with the use of IN to reduce fractures. 67 cases were selected in which IN were used to reduce fractures of dogs, which were evaluated clinically e radiographically regarding the time and type of consolidation, early deambulation, complications and limb function. The data obtained was presented in the form of descriptive statistics. It was possible to conclude that IN is an intramedullary implant that allows early return to function as it supports the load placed in the bone during walking, thus allowing the micromovement of the fracture focus, leading to secundary consolidation.
{"title":"RESULTADOS CLÍNICOS E RADIOGRÁFICOS DA REDUÇÃO DE FRATURAS POR HASTE BLOQUEADA EM 67 CÃES","authors":"Marcella Dall Agnol Leite, F. C. Mota, A. E. Alves, G. M. Nogueira, M. M. Mantovani, Matheus Franco da Veiga Teixeira","doi":"10.5380/AVS.V26I3.78122","DOIUrl":"https://doi.org/10.5380/AVS.V26I3.78122","url":null,"abstract":"Fractures in dogs are one of the great causes of care in small animal surgical clinic. The Interlocking Nail (IN) is a widely used implant, they resist flexion, torsion and axial compression forces. The present study aims to evaluate the time of consolidation, formation of bone callus, and possible complications with the use of IN to reduce fractures. 67 cases were selected in which IN were used to reduce fractures of dogs, which were evaluated clinically e radiographically regarding the time and type of consolidation, early deambulation, complications and limb function. The data obtained was presented in the form of descriptive statistics. It was possible to conclude that IN is an intramedullary implant that allows early return to function as it supports the load placed in the bone during walking, thus allowing the micromovement of the fracture focus, leading to secundary consolidation.","PeriodicalId":8351,"journal":{"name":"Archives of Veterinary Science","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45909116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}