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Turmeric Extracts as a Protective Natural Compound Against Hepatotoxicity Induced by Lead Nitrate in Male Albino Rat Model 姜黄提取物作为一种天然保护性化合物对抗硝酸铅诱导的雄性白化大鼠肝毒性
Pub Date : 2021-12-30 DOI: 10.21608/ajs.2021.75154.1374
K. Mahmoud, Medhat Mostafa Abozid, A. F. Abd El-Fattah
: Excessive exposure to lead may damage the liver by increasing oxidative stress, leading to many serious diseases. Dietary antioxidants may protect the liver from damage. Therefore, the effect of ethanolic and aqueous turmeric extracts against lead-induced liver damage was evaluated by esti-mating liver function and antioxidant/oxidative stress biomarkers. Forty rats were divided into group (A) served as control, group (B) treated with 93 mg/kg b.wt. lead nitrate, group (C) and group (D) treated as group B in addition to 400 mg/kg b.wt. of turmeric aqueous and ethanolic extracts, respec-tively. Group B showed a significant increase in the activities of alanine transaminase by 10 folds, aspartate transaminase by 9 folds, alkaline phosphatase by 3 folds and the level of malondialdehyde by 10 folds, while showed a significant decrease in albumin by 60%, total protein by 54.5%, and superoxide dismutase by 80.5%, catalase by 75% and glutathione by 71.6% in comparison with control group. On the
:过量接触铅可能会增加氧化应激,从而损害肝脏,导致许多严重疾病。饮食中的抗氧化剂可以保护肝脏免受损伤。因此,通过评估肝功能和抗氧化/氧化应激生物标志物来评估姜黄乙醇提取物和水提取物对铅诱导的肝损伤的影响。将40只大鼠分为(A)组作为对照,(B)组用93mg/kg B.wt的硝酸铅处理,(C)组和(D)组作为B组,除此之外,分别用400mg/kg B.wt的姜黄水提取物和乙醇提取物处理。与对照组相比,B组丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶和丙二醛的活性显著提高了10倍、9倍、3倍和10倍,白蛋白、总蛋白、超氧化物歧化酶、过氧化氢酶和谷胱甘肽的活性显著降低了60%、54.5%、80.5%、75%和71.6%。关于
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引用次数: 0
Identification and Reconsidering Phylogeny of Some Aphid Species, (Hemiptera: Aphididae), Based on Molecular DNA Markers Using ISSRs-PCR Technique 基于ISSRs-PCR分子DNA标记的蚜虫系统发育鉴定与再思考(半翅目:蚜科)
Pub Date : 2021-12-30 DOI: 10.21608/ajs.2021.94272.1414
I. Adss, R. Tabikha
: Aphids are considered as most economically importance and worldwide insect pests. Successful pest management systems are based on accurate and rapid pests' species identification. Traditional morphological identification of closed aphid species may be considered as inaccurate taxonomic process. For overcoming disadvantages of traditional morphological identification, molecular techniques, related to DNA markers and based on polymerase chain reaction (PCR), were approached by using nine ISSRs primers to identify and diagnose fifteen common aphid species that disperse in Egyptian agro-ecosystem. The examined ISSRs primers could successfully discriminate the tested aphid species that reflected 61.39% polymorphism among them. Moreover, four banding patterns were considered as unique bands which could characterize three aphid species ( Aphis gossypii , Aphis nerii and Myzus persicae ). Highest genetic homology (84.9%) was observed between species Rhopalosipum padi and Schizaphis graminum . In additions, each of A. gossypii and Aphis citricola were also genetically homologous species. In contrast, species Aphis craccivora and M. persicae were analogous genetically with low similarity percentile (59.8%).
蚜虫被认为是世界范围内最具经济价值的害虫。成功的病虫害管理系统是建立在准确和快速的病虫害种类识别的基础上的。传统的封闭蚜虫形态鉴定可能被认为是不准确的分类过程。为了克服传统形态鉴定方法的不足,采用基于DNA标记和聚合酶链反应(PCR)的分子技术,利用9条ISSRs引物对埃及农业生态系统中分布的15种常见蚜虫进行鉴定和诊断。所检测的ISSRs引物能够成功区分被测蚜虫种,其多态性为61.39%。此外,我们还发现了棉蚜(Aphis gossypii)、麻蚜(Aphis nerii)和桃蚜(Myzus persicae) 3种蚜虫的独特条带。两种间遗传同源性最高(84.9%)。此外,棉蚜和柠檬蚜在遗传上也是同源种。猕猴桃蚜与桃蚜遗传相似,相似百分位数较低(59.8%)。
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引用次数: 0
Producing of biodegradable plastic films from un-marketable potato tubers 用不畅销的马铃薯块茎生产可生物降解塑料薄膜
Pub Date : 2021-12-30 DOI: 10.21608/ajs.2021.92037.1409
aya kobash, fathi abdel-hadi, ashrf anwer, M. Moustafaa
: This study was conducted to reduce the volume of solid wastes by using unmarketable potato tubers for producing biodegradable plastic films to be substitute oil based plastics “OBP” which is often not biodegradable. Starch based plastic films “SBP” were produced with different concentrations of glycerol (5, 10,15, 20,25, 30, 99.5%v/v) as a plasticizer. The physical properties of SBP including thickness, density, water absorption, biodegradability, were studied, as well as mechanical properties including tensile strength, modulus of elasticity, elongation % and the required energy for firm were also investigated compared with OBP samples. The results showed that the maximum value of extracted starch was 13.5% obtained by blending potato tubers. SBP films with elevated glycerol concentration increased the sample thickness but decreased its density. Glycerol 20% gave the best flexi-ble compact structure SBP films. Average thickness and density SBP film (20% glycerol) were 0.25 mm and 80.11 Kg/m 3 respectively. Meanwhile, the
:本研究旨在通过使用未上市的马铃薯块茎生产可生物降解塑料薄膜来代替通常不可生物降解的油基塑料“OBP”来减少固体废物的体积。以不同浓度的甘油(5,10,15,20,25,30,99.5%v/v)为增塑剂,制备了淀粉基塑料薄膜“SBP”。研究了SBP的物理性能,包括厚度、密度、吸水性、生物降解性,以及与OBP样品相比的机械性能,包括拉伸强度、弹性模量、伸长率和所需能量。结果表明,马铃薯块茎的混合提取淀粉的最大值为13.5%。甘油浓度升高的SBP膜增加了样品的厚度,但降低了其密度。20%的甘油可制备出最佳的柔性致密结构SBP薄膜。SBP膜(20%甘油)的平均厚度和密度分别为0.25mm和80.11Kg/m3。同时
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引用次数: 0
Impact Of Microbial Synergism On Second Generation Production Of Bioethanol From Fruit Peels Wastes 微生物协同作用对果皮废料第二代生物乙醇生产的影响
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.75553.1376
K. Mahmoud, S. Fahim
Fruit peels annually accumulate in huge amounts; fruit wastes are rich in lignocellulosic component which can be recovered into mono simple carbohydrates able to utilize for bioethanol production. The enzymatic hydrolysis of lignocelluloses is known to be a key to the second-generation biofuel, the challenge is the still expensive enzymes involved in the saccharification process, loss of the most hemicellulose pentose sugars which were non-fermentable as base, beside the presence of hampers lignin thus require to resolve its problem. As part of study, followed the cost-effective means for bioethanol producing from four fruit peels (Banana, Orange, Mango and Watermelon), yielding of enzymes from co-fungal cultivation carried by Aspergillus niger and Phanerochaete chrysosporium, followed by co-fermentation of the saccharified sugars using yeast belong to Saccharomyces cerevisiae and Kluyveromyces marxianus which contributed for bioethanol production in 8 L lab-scale reactor. The fermented sugars recovering was reached to 27.77 g.l-1 from banana peels which were found to be good exploited as potential raw source, co-fungal enzymatic hydrolysis followed by yeast co-fermentation led to substantial yield by 10.74 g.l-1, the adding of calcium oxide increased the purity which leads finally to 97.5 wt % of pure bioethanol. Thrust towards fossil fuels replacement with renewable clean fuels such as bioethanol by using fruit peels residuals which are considered renewable energy source may also help in CO2 mitigation.
果皮每年积累大量;水果废弃物富含木质纤维素成分,可回收为可用于生物乙醇生产的单一简单碳水化合物。众所周知,木质纤维素的酶促水解是第二代生物燃料的关键,挑战在于糖化过程中涉及的酶仍然昂贵,除了存在阻碍木质素之外,失去了大多数不能发酵的半纤维素戊糖作为碱,因此需要解决其问题。作为研究的一部分,遵循从四种果皮(香蕉、橙子、芒果和西瓜)生产生物乙醇的成本效益方法,从黑曲霉和黄孢原毛平革菌携带的共真菌培养中生产酶,然后使用属于酿酒酵母(Saccharomyces cerevisiae)和马氏克鲁维酵母(Kluyveromyces marxianus)的酵母共同发酵糖化糖,这有助于在8L实验室规模的反应器中生产生物乙醇。从香蕉皮中回收的发酵糖达到27.77 g.l-1,这是一个很好的潜在原料来源,共真菌酶水解和酵母共发酵使产量达到10.74 g.l-1。添加氧化钙提高了纯度,最终获得97.5 wt%的纯生物乙醇。通过使用被视为可再生能源的果皮残渣,用生物乙醇等可再生清洁燃料取代化石燃料,也可能有助于缓解二氧化碳排放。
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引用次数: 0
DNA Barcoding of Commercial Three High Value Red Sea Fishes from the Egyptian Market 埃及市场三种商业高价值红海鱼类的DNA条形码
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.68600.1352
rana gamaleldin, M. A. Rashed, Mahmoud Magdy
Fish play an essential role in food security and are regarded as one of Egypt's primary food resources and, therefore, significantly im-pact the national economy. The three main sources of fish production in Egypt: marine (Red and Mediterranean seas), lakes and the River Nile, due to the elevated incidence of species substitution at the global level, precise identification of seafood species on the markets considered a solution for food safety control institutions and human consumer protec-tion. Mislabeling occurs when one species is substituted for another. Different species of red sea marine fish were collected from the Egyptian market. According to the Arabic name in the fish market, Samples searched for English and Latin family names in (www.fishdata-base.org). Commercial samples were successfully extracted and confirmed with 1.5% agarose gel electrophoresis. The result showed that the mtDNA gene cytochrome c oxidase subunit I (COI) amplification was successful for all samples with high concentration, it was purified and sequenced for both directions and blasted using the NCBI database. Resulting in the identification of three commercially essential and expensive fish samples, Baghbaghan (parrotfish), Hamour (Greasy Grouper) and kahaya (Spiny squirrelfish). One of them correctly identified according to the market name, while the other two are recorded mistakenly under another name.
鱼类在粮食安全中发挥着至关重要的作用,被视为埃及的主要粮食资源之一,因此对国家经济有重大影响。埃及的三个主要鱼类生产来源:海洋(红海和地中海)、湖泊和尼罗河,由于全球范围内物种替代的发生率较高,在市场上准确识别海鲜物种被认为是食品安全控制机构和人类消费者保护的解决方案。当一个物种被另一个物种取代时,就会出现标签错误。从埃及市场采集了不同种类的红海海鱼。根据鱼类市场上的阿拉伯语名称,Samples在(www.fishdata-base.org)中搜索英语和拉丁语家族名称。商业样品成功提取,并用1.5%琼脂糖凝胶电泳进行确认。结果表明,mtDNA基因细胞色素c氧化酶亚基I(COI)在所有高浓度样品中都能成功扩增,并利用NCBI数据库对其进行双向纯化和测序。由此鉴定出三种商业上必需且昂贵的鱼类样本,即Baghbaghan(鹦嘴鱼)、Hamour(Greasy Grouper)和kahaya(Spiny squirlefish)。其中一个根据市场名称正确识别,而另外两个则错误地记录在另一个名称下。
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引用次数: 0
Effect of Fungicide, Salicylic Acid, Henna Powder and Propolis on Silkworm Larvae Infected with Aspergillus sp. 杀菌剂、水杨酸、指甲花粉和蜂胶对家蚕曲霉感染的影响。
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.74341.1371
Sawsan M. Abdelmegeed
Mulberry silkworm, Bombyx mori L. is infected with many diseases. In this work, two fungi species, Aspergillus fumigatus and Aspergillus flavus , were isolated from the infected B. mori larvae during rearing process. The morphological symptoms were the same for both fungi and appeared from newly hatched larvae until moth formation. In larvae, black spots of both fungi species appeared eve-rywhere on the body, then these spots extended and eventually larvae died, and fragments of the fungus came out from the spots. The only one difference between the two fungi, is that symptoms of A. fumigatus appeared four days earlier than those of A. flavus . Newly hatched larvae were sprayed with spore suspension (1x10 6 conidia spores/ml) of A. fumigates and A. flavus separately. One and 12 hrs. after inoculation, larvae were sprayed with different concentrations of fungicide (Actamyl), salicylic acid, and henna and propolis powders, in isolation. After words, mortality and survival percentages were calculated and compared to those that occurred in untreated check (larvae were inoculated with A. fumigatus and A. flavus separately without any control treatment). The lowest mortality and highest survival per-centage were occurred in larvae treated with salicylic acid at 10% after 12 hrs. from inoculation with A. fumigatus . On the other hand, the lowest mortality and highest survival percentages occurred in larvae treated with the fungicide Actamyl at 6% and henna at 15% after 12 hrs. from inoculation with A. flavus.
桑蚕感染多种疾病。在饲养过程中,从受感染的家蚕幼虫中分离出烟曲霉和黄曲霉两种真菌。两种真菌的形态症状相同,从刚孵化的幼虫到蛾类形成都会出现。在幼虫身上,这两种真菌的黑斑都出现在身体的任何地方,然后这些斑点延伸,最终幼虫死亡,真菌的碎片从斑点中出来。这两种真菌之间唯一的区别是烟曲霉的症状比黄曲霉早四天出现。用烟曲霉和黄曲霉的孢子悬浮液(1x10-6分生孢子/ml)分别喷洒初孵幼虫。接种后1小时和12小时,幼虫分别喷洒不同浓度的杀菌剂(Act戊酯)、水杨酸、指甲花和蜂胶粉末。术后,计算死亡率和存活率,并将其与未经处理的对照组(幼虫分别接种烟曲霉和黄曲霉,不进行任何对照处理)进行比较。用10%水杨酸处理的幼虫在接种烟曲霉12小时后死亡率最低,存活率最高。另一方面,用杀菌剂Act戊酯6%和指甲花15%处理的幼虫在接种黄曲霉12小时后死亡率最低,存活率最高。
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引用次数: 1
Evaluation of different control methods on the rust flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) 不同防治方法对锈粉甲虫(鞘翅目:拟甲科)的影响
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.71422.1358
S. El-Deeb
The presented study aimed to evaluate the different control methods of the rusty flour beetle Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The experiments were conducted to evaluate the efficiency of a chemical pesticide (Mala-thion), biopesticide (Tracer 24% SC), plant oil (Lemongrass oil), plant extraction (Ac-etone extracts from Rosemary), powder (Diatomaceous earth) and modified Atmosphere (argon gas). Mortality percentage increased with increase of exposure times at all tested concentrations. The toxicity effect of different treatments against adults of T. castaneum at the LC 50 after 7 days post-treatment at 30±1°C could be arranged in descending order as follows: Malathion, Tracer 24% and lemongrass oil, while, acetone extracts from Rosemary and diatomaceous earth (DE) were the least effective. The LT 50 values against the adult stage of T. castaneum were 19.99, 2.33 and 4.86 hr. for Argon 100, 80 and 60%, respectively. In order to avoid the disadvantages of using chemical insecticides such as malathion, it is recommended to use other more safe control methods for the environment as Tracer 24%
本研究旨在评价不同防治方法对锈粉甲虫(鞘翅目:拟甲科)的影响。采用化学农药(马拉硫离子)、生物农药(示踪剂24% SC)、植物油(柠檬草油)、植物提取物(迷迭香ac -酮提取物)、粉末(硅藻土)和改性气氛(氩气)进行了效果评价。在所有测试浓度下,死亡率随暴露时间的增加而增加。在30±1℃条件下处理7 d后,不同处理对lc50条件下的成虫的毒性作用由大到小依次为:马拉硫磷、24%示踪剂和柠檬草油,而迷迭香丙酮提取物和硅藻土(DE)的毒性作用最低。对木栗螟成虫期的l50值分别为19.99、2.33和4.86 hr。分别为argon100、80和60%。为避免使用马拉硫磷等化学杀虫剂的弊端,建议使用其他对环境更安全的防治方法,如示踪剂24%
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引用次数: 0
Effect of Different Dried Methods and Storage on Some Enzymes Activity and Quality of Cactus pear and Papaya 不同干燥方法和贮藏方式对仙人掌梨和木瓜某些酶活性及品质的影响
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.77066.1382
Marwa Abdel-Hady, M. El-Shemy, S. Mahdy, E. Rizk
This study was to evaluate the activity of peroxidase, polyphenoloxidase, pectin methyl esterase and protease as well as the proximate chemical composition and quality parameters of cactus pear and papaya which were dried by different methods and stored for 6 months. Our findings revealed that the highest moisture content was recorded in osmo – freeze drying of papaya and cactus pear. On the other hand, the browning index of oven dried papaya and cactus pear was higher than those dried with osmotic dehydration and osmo – freeze drying. The less activity of peroxidase was recorded in osmo – freeze drying of papaya and cactus pear. Also, the highest reduction of polyphenoloxidase activity was noticed in osmo–freeze drying methods. While oven dried papaya recorded the highest activity of pectin methyl esterase pectin (0.34 %), complete inactivation was shown in other dried methods after 6 months of storage at 4 ±1℃. Different dried methods of papaya caused to reduce the activity of protease; moreover, complete inactivation was observed after 3 months from storage. The quality parameters for sensory evaluation of dried cactus pear and papaya demonstrated that osmo–freeze drying was more favorable to preserve samples followed by osmotic dehydration and oven drying
本研究对不同干燥方法贮藏6个月的仙人掌梨和番木瓜的过氧化物酶、多酚氧化酶、果胶甲基酯酶和蛋白酶活性以及主要化学成分和质量参数进行了评价。我们的研究结果表明,木瓜和仙人掌梨的渗透冷冻干燥中水分含量最高。另一方面,烘干木瓜和仙人掌梨的褐变指数高于渗透脱水和渗透冷冻干燥。木瓜和仙人掌梨的渗透冷冻干燥过程中过氧化物酶活性较低。此外,在渗透-冷冻干燥方法中,多酚氧化酶活性的降低最高。烘干木瓜的果胶甲基酯酶果胶活性最高(0.34%),而其他烘干方法在4±1℃下储存6个月后表现出完全失活。不同干燥方法导致木瓜蛋白酶活性降低;此外,储存3个月后观察到完全失活。仙人掌梨和木瓜干感官评价的质量参数表明,渗透冷冻干燥更有利于保存样品,其次是渗透脱水和烘箱干燥
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引用次数: 0
Assessment of some essential oils and their mixtures as materail repellents against the confused flour beetle, adult Tribolium confusum (du val) (Coleoptera: Tenebrionidae). 几种精油及其混合剂对褐粉甲虫成虫的驱避效果评价(鞘翅目:拟甲科)。
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.68479.1351
Salwa Pasha
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引用次数: 0
Effect of some growth regulators and systemic copper complexes on reducing floral malformation, yield and quality of "Alphonse" mango (Mangifera indica L. ) 一些生长调节剂和系统铜配合物对“Alphonse”芒果(Mangifera indica L.)减少花畸形、产量和品质的影响
Pub Date : 2021-09-07 DOI: 10.21608/ajs.2021.78941.1386
ايمني فريد ابو حديد, نظمي عبد الحميد عبد الغني, Samah Nasr, Abdelaty Aboelezz
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引用次数: 0
期刊
Arab Universities Journal of Agricultural Sciences
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