Pub Date : 2021-09-07DOI: 10.21608/ajs.2021.75205.1375
H. Sayed, H. Gado, Hamdy M. Metwally, A. Abdelhafez
The effect of two probiotic products with Palm Kernel Cake (PKC) as source of dietary protein and/or energy in ruminant diets on the growth performance and some blood parameters of Barki lamps were evaluated. Two commercial probiotic formulas (ZAD and ICEC-Trol) were administrated directly with theirapplications:P1 (without probiotic), P2 (ZAD), P3 (ZAD + ICEC-Trol), the combination of four different rations: R1 (control), R2 (PKC as source of energy), R3 (PKC as source of energy and protein) and R4 (PKC as source of protein). Barki lambs (n=96; 35kg average body weight) were randomly assigned to 12 experimental groups in a completely randomised block design. (8 lambs for each group) and fed for 47 days. ZAD probiotic was applied at 2 ml/head/day and ICEC-TROL was applied at 3 g/head/day. Animals weight gain and average daily gain were measured and Glucose, total protein, albumin, calcium and phosphorus were measured. Results showed that animals fed control ration (R1) with P2, R2 with P3 and R4 with P3 showed highest (P<0.05) total weight gain (10.75, 10.88 and 10.56, respectively) and average daily gain (0.229, 0.231 and 0.225, respectively). ZAD probiotic improved serum total protein, globulin, glucose when using with PKC in lambs’ rations. In conclusion, using PKC as a source of energy or protein, with two sources of probiotics in lambs’ rations showed to be more effective in weight gain, while using one probiotic (ZAD) is enough for fattening with traditional rations.
{"title":"Effect of Palm kernel cake and probiotics on growth performance of growing Barki lambs","authors":"H. Sayed, H. Gado, Hamdy M. Metwally, A. Abdelhafez","doi":"10.21608/ajs.2021.75205.1375","DOIUrl":"https://doi.org/10.21608/ajs.2021.75205.1375","url":null,"abstract":"The effect of two probiotic products with Palm Kernel Cake (PKC) as source of dietary protein and/or energy in ruminant diets on the growth performance and some blood parameters of Barki lamps were evaluated. Two commercial probiotic formulas (ZAD and ICEC-Trol) were administrated directly with theirapplications:P1 (without probiotic), P2 (ZAD), P3 (ZAD + ICEC-Trol), the combination of four different rations: R1 (control), R2 (PKC as source of energy), R3 (PKC as source of energy and protein) and R4 (PKC as source of protein). Barki lambs (n=96; 35kg average body weight) were randomly assigned to 12 experimental groups in a completely randomised block design. (8 lambs for each group) and fed for 47 days. ZAD probiotic was applied at 2 ml/head/day and ICEC-TROL was applied at 3 g/head/day. Animals weight gain and average daily gain were measured and Glucose, total protein, albumin, calcium and phosphorus were measured. Results showed that animals fed control ration (R1) with P2, R2 with P3 and R4 with P3 showed highest (P<0.05) total weight gain (10.75, 10.88 and 10.56, respectively) and average daily gain (0.229, 0.231 and 0.225, respectively). ZAD probiotic improved serum total protein, globulin, glucose when using with PKC in lambs’ rations. In conclusion, using PKC as a source of energy or protein, with two sources of probiotics in lambs’ rations showed to be more effective in weight gain, while using one probiotic (ZAD) is enough for fattening with traditional rations.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46556508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-07DOI: 10.21608/ajs.2021.52876.1308
Basma Abdel-Aziz Elsawy, I. Elsyed, Ayman Ahmed, Yaser Elbadwy
Our study was carried out at the farm of Faculty of Agriculture, Ain Shams University, Egypt. The average temperatures were 18.4°C and 23.9 °C from December 2017 - February 2018 to study the effects of Saccharomyces cerevisiae boulardii supplementation (as feed additives) on productive performance of growing rabbits. Sixty growing rabbits of the NZW (New Zealand White Rabbits), (4 weeks old) and weighted (603.62 gm mean body weight) were randomly distributed into 4 treated groups, n=15 rabbits per each group. Rabbits of supplemented groups were given 100 (R1), 200 (R2) and 400 (R3) gm/ton feed of Saccharomyces cerevisiae boulardii mixed in the diet for 6 successive weeks. Individual live body weight was noted weekly from start till the end of the experiment. At the end of the experimental period, 10 rabbits (from each group) were fasting for 12 hrs, then slaughtered for carcass traits measure. Experimental lighting system was 16-hours photoperiod /day (16L: 8D) through both daily natural and the artifi-cial fluorescent lighting. A pelleted ration of 17.3% crude protein, 13.37% crude fiber and 2510 kcal of digestible energy per kg feed was offered ad libitum all the time. Results revealed significant (P≤0.05) increases in the final body weight and daily weight gain with saccharomyces cerevisiae supplementation when compared with control group. Though, significantly (P≤0.05) improved feed conversion ratio in all treated groups compared with control one were noticed. Feed intake was the lower in treated groups than control group. There were significant (P≤0.05) increasing in hot carcass weight and carcass percentage with respect to the control group. Supplemented Saccharomyces cerevisiae reduced (P≤0.05) the liver, heart, lungs and kidneys percentages compared the control group. Supplemented rabbit feeds with saccharomyces cerevisiae boulardii in their diets had highly increasing in relative economic efficiency and clear improvement of net revenue than control group.
{"title":"Impact of Saccharomyces Cerevisiae Boulardii Supplementation As Probiotic On Productive Performance And Economic Efficiency Of Growing Rabbits","authors":"Basma Abdel-Aziz Elsawy, I. Elsyed, Ayman Ahmed, Yaser Elbadwy","doi":"10.21608/ajs.2021.52876.1308","DOIUrl":"https://doi.org/10.21608/ajs.2021.52876.1308","url":null,"abstract":"Our study was carried out at the farm of Faculty of Agriculture, Ain Shams University, Egypt. The average temperatures were 18.4°C and 23.9 °C from December 2017 - February 2018 to study the effects of Saccharomyces cerevisiae boulardii supplementation (as feed additives) on productive performance of growing rabbits. Sixty growing rabbits of the NZW (New Zealand White Rabbits), (4 weeks old) and weighted (603.62 gm mean body weight) were randomly distributed into 4 treated groups, n=15 rabbits per each group. Rabbits of supplemented groups were given 100 (R1), 200 (R2) and 400 (R3) gm/ton feed of Saccharomyces cerevisiae boulardii mixed in the diet for 6 successive weeks. Individual live body weight was noted weekly from start till the end of the experiment. At the end of the experimental period, 10 rabbits (from each group) were fasting for 12 hrs, then slaughtered for carcass traits measure. Experimental lighting system was 16-hours photoperiod /day (16L: 8D) through both daily natural and the artifi-cial fluorescent lighting. A pelleted ration of 17.3% crude protein, 13.37% crude fiber and 2510 kcal of digestible energy per kg feed was offered ad libitum all the time. Results revealed significant (P≤0.05) increases in the final body weight and daily weight gain with saccharomyces cerevisiae supplementation when compared with control group. Though, significantly (P≤0.05) improved feed conversion ratio in all treated groups compared with control one were noticed. Feed intake was the lower in treated groups than control group. There were significant (P≤0.05) increasing in hot carcass weight and carcass percentage with respect to the control group. Supplemented Saccharomyces cerevisiae reduced (P≤0.05) the liver, heart, lungs and kidneys percentages compared the control group. Supplemented rabbit feeds with saccharomyces cerevisiae boulardii in their diets had highly increasing in relative economic efficiency and clear improvement of net revenue than control group.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48253428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-07DOI: 10.21608/ajs.2021.67476.1348
Reham M Abdallah, Ibrahim Rizk Sayed Ahmed, Amal A. Hassan, hemmat Elshesheetawy
The influence of chilling, freezing, and drying preservation methods on morphological properties and viability of four yeast cultures, isolated Saccharomyces boulardii (SB1 and SB2), Saccharomyces cerevisiae (SC), and Kluyveromyces lactis (KL), was studied. chilling preservation had no effect on yeast colony morphology parameters till the end of the preservation period in contrast with freezing and drying preservation methods. Drying preservation resulted in the highest viability decrement % of yeast cultures ranged between 59.38 to 40.21% as compared with other preservation methods. There is a noticeable reduction in D values of preserved yeast cultures by drying, which varied from (33 to 26 day) in comparison to chilling and freezing preservation that recorded (119 to 83 and 180 to 57 day), respectively. The obtained results revealed that chilling preservation under proper conditions, as well as freezing, is more preferable for the preservation of studied yeast cultures than that preserved yeast by the drying method.
{"title":"A comparative study between different preservation methods on the viability of some yeast cultures","authors":"Reham M Abdallah, Ibrahim Rizk Sayed Ahmed, Amal A. Hassan, hemmat Elshesheetawy","doi":"10.21608/ajs.2021.67476.1348","DOIUrl":"https://doi.org/10.21608/ajs.2021.67476.1348","url":null,"abstract":"The influence of chilling, freezing, and drying preservation methods on morphological properties and viability of four yeast cultures, isolated Saccharomyces boulardii (SB1 and SB2), Saccharomyces cerevisiae (SC), and Kluyveromyces lactis (KL), was studied. chilling preservation had no effect on yeast colony morphology parameters till the end of the preservation period in contrast with freezing and drying preservation methods. Drying preservation resulted in the highest viability decrement % of yeast cultures ranged between 59.38 to 40.21% as compared with other preservation methods. There is a noticeable reduction in D values of preserved yeast cultures by drying, which varied from (33 to 26 day) in comparison to chilling and freezing preservation that recorded (119 to 83 and 180 to 57 day), respectively. The obtained results revealed that chilling preservation under proper conditions, as well as freezing, is more preferable for the preservation of studied yeast cultures than that preserved yeast by the drying method.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48565549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-09DOI: 10.21608/AJS.2021.71112.1357
Samah H. Abu-Hussien, Mahmoud Sameh, M. A. El-Naga
The aim of this study was to optimize the production of polyunsaturated fatty acids (PUFAs), using use the response surface methodology (RSM), to use the produced PUFAs in kareish cheese processing. Plackett Burman design (PBD) was employed to screen media components that affect PUFAs development (glucose, olive cake, yeast extract, tryptone, MgSO4, KH2PO4, NH4Cl, agitation speed, incubation time, and pH), and results showed that olive cake and yeast extract, with confidence level > 98%, had a positive effect on PUFAs production. The central composite design (CCD) of the response surface methodology was used to optimize the selected parameters levels where maximum PUFAs production (1790 mg/l) was observed near the mid-point (0) values (concentrations) of olive cake (15 g/l), tryptone (7.5 g/L) and KH2PO4 (1.25 g/l). Polyunsaturated fatty acids account for 47.83 % of the total fatty acid profile, according to gas chromatography analysis of the collected PUFAs. The produced PUFAs was encapsulated using whey protein concentrate and maltodextrin, freeze dried, grinded and incorporated in Kareish cheese manufacture. The average particle size of a 0.005% suspension of oil microcapsules was 671.4 nm with a poly dispersity index of 0.611 indicating a moderate stability of the emulsion. The negative zeta potential of the microcapsules particles was -37.6 mv, which is identical to the -42 mv value recorded in the literature for oil emulsions stabilized by whey protein, maltodextrin, and K-carrageenan. The addition of 0.5 % PUFAs-containing microcapsules to Kareish cheese increased antioxidative activity to 38.13 % compared to 30.14 % for the control, as well as Texture profile analysis (TPA) parameters including hardness, cohesiveness, gumminess, and chewiness. The elasticity of the Kareish cheese sample increased slightly by the addition of 0.5% microcapsules, but higher concentration tended to change the elasticity to a brittleness of the cheese structure.
{"title":"Optimization of Polyunsaturated Fatty Acids (PUFAs) Production from Olive Cake by Pseudomonas fluorescens NBRC14160 using Response Surface Methodology and their Application in Kareish Cheese Manufacture","authors":"Samah H. Abu-Hussien, Mahmoud Sameh, M. A. El-Naga","doi":"10.21608/AJS.2021.71112.1357","DOIUrl":"https://doi.org/10.21608/AJS.2021.71112.1357","url":null,"abstract":"The aim of this study was to optimize the production of polyunsaturated fatty acids (PUFAs), using use the response surface methodology (RSM), to use the produced PUFAs in kareish cheese processing. Plackett Burman design (PBD) was employed to screen media components that affect PUFAs development (glucose, olive cake, yeast extract, tryptone, MgSO4, KH2PO4, NH4Cl, agitation speed, incubation time, and pH), and results showed that olive cake and yeast extract, with confidence level > 98%, had a positive effect on PUFAs production. The central composite design (CCD) of the response surface methodology was used to optimize the selected parameters levels where maximum PUFAs production (1790 mg/l) was observed near the mid-point (0) values (concentrations) of olive cake (15 g/l), tryptone (7.5 g/L) and KH2PO4 (1.25 g/l). Polyunsaturated fatty acids account for 47.83 % of the total fatty acid profile, according to gas chromatography analysis of the collected PUFAs. The produced PUFAs was encapsulated using whey protein concentrate and maltodextrin, freeze dried, grinded and incorporated in Kareish cheese manufacture. The average particle size of a 0.005% suspension of oil microcapsules was 671.4 nm with a poly dispersity index of 0.611 indicating a moderate stability of the emulsion. The negative zeta potential of the microcapsules particles was -37.6 mv, which is identical to the -42 mv value recorded in the literature for oil emulsions stabilized by whey protein, maltodextrin, and K-carrageenan. The addition of 0.5 % PUFAs-containing microcapsules to Kareish cheese increased antioxidative activity to 38.13 % compared to 30.14 % for the control, as well as Texture profile analysis (TPA) parameters including hardness, cohesiveness, gumminess, and chewiness. The elasticity of the Kareish cheese sample increased slightly by the addition of 0.5% microcapsules, but higher concentration tended to change the elasticity to a brittleness of the cheese structure.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46148379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.49712.1298
Shaimaa Abd Elhady, A. Sharaf, A. Salam, Khaled Abd El Atey, A. A. Shady
A crucial menace for the biodiversity in arid and semi-arid territories is the global warming arising from anthropogenic activity. Egypt is expected to undergo an acute rainfall decrease and temperature boost in the next few decades, leading to many plant species' geographical allocation. Endemic plants of pleiotropic economic importance are strongly affected by climate change prospects, which will gradually result in losing our plant wealth genetic resources. Due to few studies on the Egyptian Brassicaceae family which has an economically and medicinally importance due to the presence of many active compounds that are included, in the pharmaceutical and cosmetic components (e.g., glucosinolates) besides having a large amount of antioxidant which inhibit the growth of microbes and also treat rheumatic diseases. The identification of this family still kind of fishy as researchers mostly relies on the morphological characters. To globally sustain this Egyptian plant family wealth's genetic pattern, it is substantial to characterize them based on their authenticated genetic background. Here we present a phylogenetic analysis of 16 species of the Egyptian Brassicaceae family using two plastid coding genes; Ribulose-1,5bisphosphate carboxylase oxygenase (rbcL-a) and maturase K (matK). The maximum likelihood of the two markers for our samples was concordant with the Brassicaceae's references-sequences, which exist on plastid are considered highly conserved biomarkers. In conclusion, we have generated a robust phylogeny tree based on the molecular level that validates the Egyptian plant species and reliably differentiates them on morphological identification. This study is considered the first phase of Egyptian Brassicaceae family species authentication followed by biochemical studies serving the pharmacological and medicinal fields.
{"title":"Phylogeny Validation for Some Egyptian Brassicaceae Endemic Species","authors":"Shaimaa Abd Elhady, A. Sharaf, A. Salam, Khaled Abd El Atey, A. A. Shady","doi":"10.21608/AJS.2021.49712.1298","DOIUrl":"https://doi.org/10.21608/AJS.2021.49712.1298","url":null,"abstract":"A crucial menace for the biodiversity in arid and semi-arid territories is the global warming arising from anthropogenic activity. Egypt is expected to undergo an acute rainfall decrease and temperature boost in the next few decades, leading to many plant species' geographical allocation. Endemic plants of pleiotropic economic importance are strongly affected by climate change prospects, which will gradually result in losing our plant wealth genetic resources. Due to few studies on the Egyptian Brassicaceae family which has an economically and medicinally importance due to the presence of many active compounds that are included, in the pharmaceutical and cosmetic components (e.g., glucosinolates) besides having a large amount of antioxidant which inhibit the growth of microbes and also treat rheumatic diseases. The identification of this family still kind of fishy as researchers mostly relies on the morphological characters. To globally sustain this Egyptian plant family wealth's genetic pattern, it is substantial to characterize them based on their authenticated genetic background. Here we present a phylogenetic analysis of 16 species of the Egyptian Brassicaceae family using two plastid coding genes; Ribulose-1,5bisphosphate carboxylase oxygenase (rbcL-a) and maturase K (matK). The maximum likelihood of the two markers for our samples was concordant with the Brassicaceae's references-sequences, which exist on plastid are considered highly conserved biomarkers. In conclusion, we have generated a robust phylogeny tree based on the molecular level that validates the Egyptian plant species and reliably differentiates them on morphological identification. This study is considered the first phase of Egyptian Brassicaceae family species authentication followed by biochemical studies serving the pharmacological and medicinal fields.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43386078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.50511.1302
Noha A. Hassaan, A. Khattab, M. Khorshed, N. El-bordeny, A. Abedo, M. Shoukry
Various fungal genotypes (Aspergillus niger NRRL 3135 (AN1), Aspergillus niger NRRL 326 (AN26), Aspergillus terrus F2-Kh (AT) and Mucor racemosus NRRL 3639 (MI)) were studied for their ability to produce phytase and improve the produced enzyme by ethyl-methane sulfonate (EMS) mutagenesis. AN1 showed the highest phytase activity on phytase screening medium supplemented with glucose (PSMG) after 8 days of incubation (reached 1875.40 IU/mL). The phytase activity of AN1 increased with increasing incubation time and the highest value was achieved at 12 days of incubation (2859.33 IU/mL). The exposure of AN1 spore suspension to 200 mM of EMS for different times enhanced the phytase activity and that mutant 20 Mn exhibited the highest phytase activity (reached 4520.5 IU/mL) therefore it was chosen for the next experiment. An in-vitro gas production procedure was carried out to evaluate the impact of using various amounts of laboratory produced phytase (PE) compared with commercial phytase (Axtra® PHY) on nutrients availability of ruminant's ration. Six levels (0, 400, 800, 1200, 1600 and 2000 IU phytaseKg dry matter) of phytase enzyme were evaluated with tested ration consisted of 40% berseem hay (BH) and 60% concentrate feed mixture (CFM). In-vitro dry and organic matter degradability (IVDMD and IVOMD), total gas production (GP), short chain fatty acids (SCFA’s) and inorganic phosphorus (Pi) concentration were improved significantly (P<0.05) by phytase addition from the two sources of phytase and the highest significant (P<0.05) values achieved at the level of 1200 IU. Results suggest that phytase activity was influenced by exposure to EMS mutagen compared to the wild type. Also, the produced enzyme source has the ability to improve the utilization efficiency of phytate diets as evidenced by the significant (P<0.05) increase in all tested parameters compared to the commercial source.
{"title":"Improvement of Fungal Phytase Production and Its In-vitro Application in Ruminant Nutrition","authors":"Noha A. Hassaan, A. Khattab, M. Khorshed, N. El-bordeny, A. Abedo, M. Shoukry","doi":"10.21608/AJS.2021.50511.1302","DOIUrl":"https://doi.org/10.21608/AJS.2021.50511.1302","url":null,"abstract":"Various fungal genotypes (Aspergillus niger NRRL 3135 (AN1), Aspergillus niger NRRL 326 (AN26), Aspergillus terrus F2-Kh (AT) and Mucor racemosus NRRL 3639 (MI)) were studied for their ability to produce phytase and improve the produced enzyme by ethyl-methane sulfonate (EMS) mutagenesis. AN1 showed the highest phytase activity on phytase screening medium supplemented with glucose (PSMG) after 8 days of incubation (reached 1875.40 IU/mL). The phytase activity of AN1 increased with increasing incubation time and the highest value was achieved at 12 days of incubation (2859.33 IU/mL). The exposure of AN1 spore suspension to 200 mM of EMS for different times enhanced the phytase activity and that mutant 20 Mn exhibited the highest phytase activity (reached 4520.5 IU/mL) therefore it was chosen for the next experiment. An in-vitro gas production procedure was carried out to evaluate the impact of using various amounts of laboratory produced phytase (PE) compared with commercial phytase (Axtra® PHY) on nutrients availability of ruminant's ration. Six levels (0, 400, 800, 1200, 1600 and 2000 IU phytaseKg dry matter) of phytase enzyme were evaluated with tested ration consisted of 40% berseem hay (BH) and 60% concentrate feed mixture (CFM). In-vitro dry and organic matter degradability (IVDMD and IVOMD), total gas production (GP), short chain fatty acids (SCFA’s) and inorganic phosphorus (Pi) concentration were improved significantly (P<0.05) by phytase addition from the two sources of phytase and the highest significant (P<0.05) values achieved at the level of 1200 IU. Results suggest that phytase activity was influenced by exposure to EMS mutagen compared to the wild type. Also, the produced enzyme source has the ability to improve the utilization efficiency of phytate diets as evidenced by the significant (P<0.05) increase in all tested parameters compared to the commercial source.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43803868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.56289.1326
M. Anous, Emanuel Kodit, H. Elhifnawy, M. A. Rashed, M. Sadek
{"title":"Inter and Intra-Breed Genetics Diversity Assessment of Three Egyptian Goat Breeds Using COI and F-AFLP Markers","authors":"M. Anous, Emanuel Kodit, H. Elhifnawy, M. A. Rashed, M. Sadek","doi":"10.21608/AJS.2021.56289.1326","DOIUrl":"https://doi.org/10.21608/AJS.2021.56289.1326","url":null,"abstract":"","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41682189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.64667.1341
M. Mohamed, M. Hegazi, Essam A. Wasif, Osama Ahmed Bedir
{"title":"Improvement of Irrigation Efficiency by Developing on Farm Irrigation System","authors":"M. Mohamed, M. Hegazi, Essam A. Wasif, Osama Ahmed Bedir","doi":"10.21608/AJS.2021.64667.1341","DOIUrl":"https://doi.org/10.21608/AJS.2021.64667.1341","url":null,"abstract":"","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46075915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.49572.1295
Ahmed B. A. Ali, Y. El-Kenany, I. Aumara, O. Aita
Nonstarter lactic acid bacteria (NSLAB) have an important role in quality and safety of traditional Baramily cheese (Domiati cheese related type). Therefore, the objective of this study was to isolate and identify NSLAB with potential technological features from traditional Baramily cheese. Thirty-three samples of Baramily cheese randomly collected from retails in Cairo metropolitan area. The samples were characterized by physiochemical, textural profile and microbiological analysis. Ninety presumptive NSLAB (30 Lactobacillus spp. and 35 Enterococcus spp.) strains were isolated on MRS and Kenner-Faecal (KF) Streptococci media; and were characterized for growth temperature, salt tolerance and milk coagulation. All presumptive NSLAB isolates were tolerant to 6.5 % NaCl. Of them, 40 isolates were tolerant to 10.0 % NaCl including 16 presumptive Lactobacillus spp., and 24 presumptive Enterococcus spp. isolates. based on the results, 11 representative isolates with potential technological features were selected for genetic identification using 16S rRNA technique, then were confirmed for growth and acidity development in skim milk within 48 h, and were tested for antimicrobial activity against some food spoilage and pathogenic microorganisms. The eleven isolates were identified as Ent. durans (1), Ent. faecalis (5), Lb. paraplantarum (1), Lb. plantarum (3), and Lb. rhamnosus (1). All isolated strains were confirmed active in skim milk, and some exhibited antimicrobial activity against tested food spoilage and pathogenic microorganisms. Both Lb. rhamnosus and Lb. plantarum were confirmed as the isolates with high activity in milk. Ent. durans and Ent. faecalis exhibited antimicrobial activity against Enterobacer aerogenes and E. coli. However, Lb. plantarum exhibited antimicrobial activity against both Enterobacer aerogenes, E. coli and Ps. aeruginosa. Listeria. monocytogenes, S. typhimurium, and Campylobacter. jejuni showed significant resistance to all tested NSLAB isolates. They can be concluded that the identified NSLAB isolated can be used to standardized and improve the quality and safety of Baramily cheese and other types related to Domiati cheese.
{"title":"Isolation and Identification of Nonstarter Lactic Acid Bacteria from Traditional Baramily Cheese","authors":"Ahmed B. A. Ali, Y. El-Kenany, I. Aumara, O. Aita","doi":"10.21608/AJS.2021.49572.1295","DOIUrl":"https://doi.org/10.21608/AJS.2021.49572.1295","url":null,"abstract":"Nonstarter lactic acid bacteria (NSLAB) have an important role in quality and safety of traditional Baramily cheese (Domiati cheese related type). Therefore, the objective of this study was to isolate and identify NSLAB with potential technological features from traditional Baramily cheese. Thirty-three samples of Baramily cheese randomly collected from retails in Cairo metropolitan area. The samples were characterized by physiochemical, textural profile and microbiological analysis. Ninety presumptive NSLAB (30 Lactobacillus spp. and 35 Enterococcus spp.) strains were isolated on MRS and Kenner-Faecal (KF) Streptococci media; and were characterized for growth temperature, salt tolerance and milk coagulation. All presumptive NSLAB isolates were tolerant to 6.5 % NaCl. Of them, 40 isolates were tolerant to 10.0 % NaCl including 16 presumptive Lactobacillus spp., and 24 presumptive Enterococcus spp. isolates. based on the results, 11 representative isolates with potential technological features were selected for genetic identification using 16S rRNA technique, then were confirmed for growth and acidity development in skim milk within 48 h, and were tested for antimicrobial activity against some food spoilage and pathogenic microorganisms. The eleven isolates were identified as Ent. durans (1), Ent. faecalis (5), Lb. paraplantarum (1), Lb. plantarum (3), and Lb. rhamnosus (1). All isolated strains were confirmed active in skim milk, and some exhibited antimicrobial activity against tested food spoilage and pathogenic microorganisms. Both Lb. rhamnosus and Lb. plantarum were confirmed as the isolates with high activity in milk. Ent. durans and Ent. faecalis exhibited antimicrobial activity against Enterobacer aerogenes and E. coli. However, Lb. plantarum exhibited antimicrobial activity against both Enterobacer aerogenes, E. coli and Ps. aeruginosa. Listeria. monocytogenes, S. typhimurium, and Campylobacter. jejuni showed significant resistance to all tested NSLAB isolates. They can be concluded that the identified NSLAB isolated can be used to standardized and improve the quality and safety of Baramily cheese and other types related to Domiati cheese.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42681416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-24DOI: 10.21608/AJS.2021.56915.1328
Esraa SeifEldin, Alia Elsaudi, Khalid Fahmy, L. M. Radwan
The present study was undertaken to test the effect of heat stress (39 °C) for 4 hours on hsp70 mRNA profiling in two chicken genotypes. Expression levels of hsp70 were used to assess the heat tolerance of two Egyptian local genotypes (Fayoumi and Matrouh). The expression level of the hsp70 gene is high in the Fayoumi type as opposed to the Matrouh type. In blood, after four hours of exposure, the hsp70 mRNA expression analysis revealed higher expression levels. These results showed that the acquired thermotolerance is positively linked to the stress memory. Within the two genotypes, Fayoumi type exhibited the highest means for hsp70 production, and therefore the heat stress is better tolerated, which indicates that Fayoumi mortality rate might be lower genetically under heat stress.
{"title":"The hsp70 EXPRESSION PROFILING IN FAYOUMI AND MATROUH CHICKEN SUBJECTED TO HEAT STRESS","authors":"Esraa SeifEldin, Alia Elsaudi, Khalid Fahmy, L. M. Radwan","doi":"10.21608/AJS.2021.56915.1328","DOIUrl":"https://doi.org/10.21608/AJS.2021.56915.1328","url":null,"abstract":"The present study was undertaken to test the effect of heat stress (39 °C) for 4 hours on hsp70 mRNA profiling in two chicken genotypes. Expression levels of hsp70 were used to assess the heat tolerance of two Egyptian local genotypes (Fayoumi and Matrouh). The expression level of the hsp70 gene is high in the Fayoumi type as opposed to the Matrouh type. In blood, after four hours of exposure, the hsp70 mRNA expression analysis revealed higher expression levels. These results showed that the acquired thermotolerance is positively linked to the stress memory. Within the two genotypes, Fayoumi type exhibited the highest means for hsp70 production, and therefore the heat stress is better tolerated, which indicates that Fayoumi mortality rate might be lower genetically under heat stress.","PeriodicalId":8366,"journal":{"name":"Arab Universities Journal of Agricultural Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44297451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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