K. Khalid, Heba M. Amer, H. Wahba, S. Hendawy, Tamer M. Abd El-Razik
{"title":"Selenium to Improve Growth Characters, Photosynthetic Pigments and Essential Oil Composition of Chives Varieties","authors":"K. Khalid, Heba M. Amer, H. Wahba, S. Hendawy, Tamer M. Abd El-Razik","doi":"10.3923/AJCS.2017.92.99","DOIUrl":"https://doi.org/10.3923/AJCS.2017.92.99","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"40 1","pages":"92-99"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91385653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Objective: Tropical maize is the most cultivated crop in sub-Saharan Africa and is a staple food to over 220 million people. This study, evaluated the competence of four tropical maize (Zea mays L.) inbred lines to callus induction and regeneration through somatic embryogenesis when co-cultivated on yeast extract peptone medium (YEP). Materials and Methods: Transient GUS assay was used to evaluate the competence of the genotypes to Agrobacterium-mediated transformation using YEP as co-cultivation media or when YEP was supplemented with 2,4-D (YEP+2,4-D), cysteine (YEP+CYS), proline (YEP+PRO) or in combination (YEP+ALL). Data on all parameters were analyzed using multivariate ANOVA and SAS. Results: Co-cultivation media based on YEP alone did not impact callus induction and immature embryos exhibited preconscious germination. When YEP was supplemented with 2,4-D at concentrations 1.5 and 3 mg LG1 2,4-D, the formation of embryogenic calli was induced and regeneration initiated. Immature embryos had high transient GUS expression when co-cultivated with Agrobacterium on YEP, YEP+PRO and YEP+ALL media than when co-cultivated on MS media, suggesting that YEP promotes Agrobacterium-mediated integration of transgenes in tropical maize. Agrobacterium tumefaciens at a concentration of 0.07 (OD660) gave the highest transient GUS expression (20.90%) while concentrations of 0.8 and 0.2 resulted in low transient GUS expression (9.17 and 12.22%, respectively). Conclusion: The integration of YEP media in the Agrobacterium-mediated transformation protocols is likely to contribute in the development of a more efficient Agrobacterium-mediated transformation system for tropical maize genotypes.
{"title":"Yeast Extract Peptone Based Co-cultivation Media Promotes Transient GUS Expression in Tropical Maize Genotypes","authors":"J. Muli, C. Mweu, N. Budambula, S. Anami","doi":"10.3923/AJCS.2017.71.81","DOIUrl":"https://doi.org/10.3923/AJCS.2017.71.81","url":null,"abstract":"Background and Objective: Tropical maize is the most cultivated crop in sub-Saharan Africa and is a staple food to over 220 million people. This study, evaluated the competence of four tropical maize (Zea mays L.) inbred lines to callus induction and regeneration through somatic embryogenesis when co-cultivated on yeast extract peptone medium (YEP). Materials and Methods: Transient GUS assay was used to evaluate the competence of the genotypes to Agrobacterium-mediated transformation using YEP as co-cultivation media or when YEP was supplemented with 2,4-D (YEP+2,4-D), cysteine (YEP+CYS), proline (YEP+PRO) or in combination (YEP+ALL). Data on all parameters were analyzed using multivariate ANOVA and SAS. Results: Co-cultivation media based on YEP alone did not impact callus induction and immature embryos exhibited preconscious germination. When YEP was supplemented with 2,4-D at concentrations 1.5 and 3 mg LG1 2,4-D, the formation of embryogenic calli was induced and regeneration initiated. Immature embryos had high transient GUS expression when co-cultivated with Agrobacterium on YEP, YEP+PRO and YEP+ALL media than when co-cultivated on MS media, suggesting that YEP promotes Agrobacterium-mediated integration of transgenes in tropical maize. Agrobacterium tumefaciens at a concentration of 0.07 (OD660) gave the highest transient GUS expression (20.90%) while concentrations of 0.8 and 0.2 resulted in low transient GUS expression (9.17 and 12.22%, respectively). Conclusion: The integration of YEP media in the Agrobacterium-mediated transformation protocols is likely to contribute in the development of a more efficient Agrobacterium-mediated transformation system for tropical maize genotypes.","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"106 1","pages":"71-81"},"PeriodicalIF":0.0,"publicationDate":"2017-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74367643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Forage Quality of Triple Maize Crops with Winter Barley in Kyushu, Japan","authors":"Yingkui Yang, B. Li, S. Idota, Y. Ishii","doi":"10.3923/AJCS.2017.1.10","DOIUrl":"https://doi.org/10.3923/AJCS.2017.1.10","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"1 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91519255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Genotypes and Potassium Rates on Some of Cowpea Traits Heritability","authors":"Radhi Dheyab Abe","doi":"10.3923/AJCS.2017.11.19","DOIUrl":"https://doi.org/10.3923/AJCS.2017.11.19","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"183 1","pages":"11-19"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75620665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-15DOI: 10.3923/AJCS.2016.96.102
H. Salama, A. Nawar
{"title":"Variations of the Cell Wall Components of Multi-cut Forage Legumes, Grasses and Legume-grass Binary Mixtures Grown in Egypt","authors":"H. Salama, A. Nawar","doi":"10.3923/AJCS.2016.96.102","DOIUrl":"https://doi.org/10.3923/AJCS.2016.96.102","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"21 1","pages":"96-102"},"PeriodicalIF":0.0,"publicationDate":"2016-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83114970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-09-15DOI: 10.3923/AJCS.2016.103.108
J. S. Hamdani, Kusumiyati, Yayat Rochayat Suradinata
{"title":"Growth and Yield of Cultivar Atlantic Potato in Medium Altitude with Paclobutrazol Application and Different Amount of Watering","authors":"J. S. Hamdani, Kusumiyati, Yayat Rochayat Suradinata","doi":"10.3923/AJCS.2016.103.108","DOIUrl":"https://doi.org/10.3923/AJCS.2016.103.108","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"99 1","pages":"103-108"},"PeriodicalIF":0.0,"publicationDate":"2016-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80978798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. A. Kadhimi, A. A. Kadhimi, C. Zain, A. N. Alhasnawi, A. Isahak, M. Ashraf, A. Mohamad, Febri Doni, W. Yusoff
Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20% polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20% PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.
{"title":"Effect of irradiation and polyethylene glycol on drought tolerance of MR269 genotype rice (Oryza sativa L.)","authors":"A. A. Kadhimi, A. A. Kadhimi, C. Zain, A. N. Alhasnawi, A. Isahak, M. Ashraf, A. Mohamad, Febri Doni, W. Yusoff","doi":"10.3923/AJCS.2016.52.59","DOIUrl":"https://doi.org/10.3923/AJCS.2016.52.59","url":null,"abstract":"Background: A drought-tolerant genotype should be identified and improved for the continuity of food security because of the global importance of rice. Mutation and tissue culture techniques are effective and important for studies on the development of potential rice resources. The effects of irradiation and drought stress on rice genotype MR269 of Oryza sativa L., differing in drought tolerance capability were investigated. Methodology: The seeds of the genotypes were exposed to gamma rays at 350 Gy. Naked seeds were initiated onto half-strength standard MS medium enhanced with 0, 10 and 20% polyethylene glycol (PEG) 6000. The effects of PEG on root and shoot length, fresh and dry seedling weights, total chlorophyll and carbohydrate contents and proline accumulation were examined. Significant differences were observed in the studied traits. The non-irradiated seeds presented the highest mean shoot and seedling fresh weight, whereas the irradiated seeds presented the largest root length and highest proline concentrations. Results: The PEG produced a decrease in the means of all the characteristics investigated by enhancing PEG concentration, except for the means of proline content, which increased with the increase in PEG concentration. The irradiated genotypes produced the highest values of root length, dry weight, chlorophyll, proline and carbohydrates in concentrations of 20% PEG compared with the non-irradiated ones. Conclusion: Thus, a positive signal exists in the effect of radiation on these characteristics in drought tolerance. Research on the improvement of a drought-tolerant genotype under different drought conditions is necessary to enhance rice productivity and food security.","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"11 1","pages":"52-59"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72788598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ramla, M. Yakhou, N. Bilek, M. Hamou, A. Hannachi, A. Aissat, L. Mekliche-H
{"title":"Grain Yield Stability Analysis of Barley Doubled Haploid Lines in Algerian Semi-arid Zones","authors":"D. Ramla, M. Yakhou, N. Bilek, M. Hamou, A. Hannachi, A. Aissat, L. Mekliche-H","doi":"10.3923/AJCS.2016.43.51","DOIUrl":"https://doi.org/10.3923/AJCS.2016.43.51","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"1 1","pages":"43-51"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80094117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Weed Survey in Sweet Corn (Zea mays saccharata Sturt.) in Regency of Sumedang and Bandung Indonesia","authors":"D. Kurniadie, U. Umiyati, D. Widayat","doi":"10.3923/AJCS.2016.66.70","DOIUrl":"https://doi.org/10.3923/AJCS.2016.66.70","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"33 1","pages":"66-70"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87935582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vurnerability Assessment of Climate Change on Agriculture Sector in the South Sumatra Province, Indonesia","authors":"Ruminta, Handoko","doi":"10.3923/AJCS.2016.31.42","DOIUrl":"https://doi.org/10.3923/AJCS.2016.31.42","url":null,"abstract":"","PeriodicalId":8504,"journal":{"name":"Asian Journal of Crop Science","volume":"68 1","pages":"31-42"},"PeriodicalIF":0.0,"publicationDate":"2016-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85573832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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