Aims: This research objective was to investigate some in-vitro properties of methanol-extracted plant extracts of Ludwigia adscendens (the water primrose). The primary method of assessment for the methanolic extract of Ludwigia adscendens (MELA) was investigated for phytochemical screening. It was determined to look into the plant's potential for in vitro activities, such as antioxidant, antimicrobial, anti-inflammatory anti-arthritis, cytotoxic, thrombolytic and membrane stabilizing activity due to the pharmaceutical interest in its component parts.
Methodology: Phytochemical screening was performed utilizing a battery of test reagents. Antioxidant, antimicrobial, anti-inflammatory, anti-arthritic, cytotoxic, thrombolytic and membrane stabilizing activities were measured at various doses using the DPPH Free Radical Scavenging Assay, the Disk Diffusion Method, Egg Albumin Protein Denaturation Assay, the Inhibition of Protein Denaturation Assay, the Brine Shrimp Lethality Bioassay, Clot lysis assay and heat induced and hypotonic induced haemolysis method.
Results: The findings of research on MELA's phytochemical screening showed the presence of a wide range of various phytochemical components. The higher antioxidant activity of MELA is shown by its IC50 value of 85.76 µg/mL. Significant antibacterial action was shown by MELA. As compared to the standard ascorbic acid, this extract showed significantly greater anti-arthritic and anti-inflammatory effects. MELA exhibited much higher LC50 value which is 1.147 µg/mL than the typical vincristine-sulphate in a lethality test with brine shrimp. Comparatively speaking, this herb has quite powerful thrombolytic action. Significant membrane stabilizing action has also been shown by MELA.
Conclusion: To wrap it up, it is clear that this plants phytochemical can be used for wide range of drug discovery field due to its potent pharmacological actions.
{"title":"In-vitro Pharmacological Investigation of Ludwigia adscendens","authors":"Md. Anik Bhuiyan, Hasib Khan Shomudro, Sadia Afreen Chowdhury","doi":"10.9734/aprj/2023/v11i6229","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i6229","url":null,"abstract":"Aims: This research objective was to investigate some in-vitro properties of methanol-extracted plant extracts of Ludwigia adscendens (the water primrose). The primary method of assessment for the methanolic extract of Ludwigia adscendens (MELA) was investigated for phytochemical screening. It was determined to look into the plant's potential for in vitro activities, such as antioxidant, antimicrobial, anti-inflammatory anti-arthritis, cytotoxic, thrombolytic and membrane stabilizing activity due to the pharmaceutical interest in its component parts.
 Methodology: Phytochemical screening was performed utilizing a battery of test reagents. Antioxidant, antimicrobial, anti-inflammatory, anti-arthritic, cytotoxic, thrombolytic and membrane stabilizing activities were measured at various doses using the DPPH Free Radical Scavenging Assay, the Disk Diffusion Method, Egg Albumin Protein Denaturation Assay, the Inhibition of Protein Denaturation Assay, the Brine Shrimp Lethality Bioassay, Clot lysis assay and heat induced and hypotonic induced haemolysis method.
 Results: The findings of research on MELA's phytochemical screening showed the presence of a wide range of various phytochemical components. The higher antioxidant activity of MELA is shown by its IC50 value of 85.76 µg/mL. Significant antibacterial action was shown by MELA. As compared to the standard ascorbic acid, this extract showed significantly greater anti-arthritic and anti-inflammatory effects. MELA exhibited much higher LC50 value which is 1.147 µg/mL than the typical vincristine-sulphate in a lethality test with brine shrimp. Comparatively speaking, this herb has quite powerful thrombolytic action. Significant membrane stabilizing action has also been shown by MELA.
 Conclusion: To wrap it up, it is clear that this plants phytochemical can be used for wide range of drug discovery field due to its potent pharmacological actions.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135243756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-01DOI: 10.9734/aprj/2023/v11i6228
D. S. H. S. Peiris, D. T. K. Fernando, S. P. N. N. Senadeera, C. B. Ranaweera
Salacia reticulata (HIPPOCRATEACEAE) is a large ligneous climber or scandent shrub held by Sri Lanka, the Andaman Islands, and south India. It is known as Kothala himbutu/Himbutu wel in Sinhalese and is well-known among natives for its extensive use in diabetes treatment, a chronic condition impacting carbohydrate, protein, and fat metabolism due to insulin secretion deficiencies, with or without insulin resistance. It is also employed in remedies for various ailments, including asthma, amenorrhea, itching and other skin problems, gonorrhoea, haemorrhoids, swellings, and obesity. Key antidiabetic compounds within S. reticulata are recognized, such as salacinol and kotalanol (from both roots and stems), and mangiferin (root-derived). Additionally, the plant's roots contain diverse chemical constituents like 1,3-diketones, 26-hydroxy-1,3-friedelanedione, 30-hydroxy-20(30) dihydroisoiguesterin, dulcitol, epicatechin, glycosidal tannins, hydroxyferruginol, iguesterin, kotalagenin 16-acetate, lambertic acid, leucopelargonidin, phlobatannin, and triterpenes, collectively contributing to its therapeutic potential. Salacia's antidiabetic effects are considered to stem primarily from its capacity to inhibit intestinal enzymes, specifically α-glucosidase and α-amylase, delaying glucose absorption and enhancing blood sugar control. Furthermore, the Salacia extracts have appeared to be very safe in vivo and in vitro experiments, and human use. The purpose of the current study is to review the botanical, ethnomedicinal, phytochemical, and pharmacological data on Salacia reticulata while assessing the evidence pertaining to in vitro, animal, and human studies for its anti-inflammatory, antibacterial, anti-diabetic, anti-obesity, anti-oxidant, and anti-hyperlipidaemic effects.
{"title":"A Comprehensive Review of Salacia reticulata: Botanical, Ethnomedicinal, Phytochemical, and Pharmacological Insights","authors":"D. S. H. S. Peiris, D. T. K. Fernando, S. P. N. N. Senadeera, C. B. Ranaweera","doi":"10.9734/aprj/2023/v11i6228","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i6228","url":null,"abstract":"Salacia reticulata (HIPPOCRATEACEAE) is a large ligneous climber or scandent shrub held by Sri Lanka, the Andaman Islands, and south India. It is known as Kothala himbutu/Himbutu wel in Sinhalese and is well-known among natives for its extensive use in diabetes treatment, a chronic condition impacting carbohydrate, protein, and fat metabolism due to insulin secretion deficiencies, with or without insulin resistance. It is also employed in remedies for various ailments, including asthma, amenorrhea, itching and other skin problems, gonorrhoea, haemorrhoids, swellings, and obesity. Key antidiabetic compounds within S. reticulata are recognized, such as salacinol and kotalanol (from both roots and stems), and mangiferin (root-derived). Additionally, the plant's roots contain diverse chemical constituents like 1,3-diketones, 26-hydroxy-1,3-friedelanedione, 30-hydroxy-20(30) dihydroisoiguesterin, dulcitol, epicatechin, glycosidal tannins, hydroxyferruginol, iguesterin, kotalagenin 16-acetate, lambertic acid, leucopelargonidin, phlobatannin, and triterpenes, collectively contributing to its therapeutic potential. Salacia's antidiabetic effects are considered to stem primarily from its capacity to inhibit intestinal enzymes, specifically α-glucosidase and α-amylase, delaying glucose absorption and enhancing blood sugar control. Furthermore, the Salacia extracts have appeared to be very safe in vivo and in vitro experiments, and human use. The purpose of the current study is to review the botanical, ethnomedicinal, phytochemical, and pharmacological data on Salacia reticulata while assessing the evidence pertaining to in vitro, animal, and human studies for its anti-inflammatory, antibacterial, anti-diabetic, anti-obesity, anti-oxidant, and anti-hyperlipidaemic effects.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135320793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-25DOI: 10.9734/aprj/2023/v11i6227
G. D. S. Pushpika, R. S. Maddumage, H. E. H. Perera, R. S. P. Fernando, A. R. N. Silva
Aims: The study aims to evaluate the albumin heat denaturalization inhibition activity of a Sri Lankan Traditional Medicine pill (STMP) as an indirect measure of anti-inflammation.
Methodology: The extracts of Aloe vera, Centella asiatica (Asiatic pennywort), and Strychnos potatorum (Cleaning nut) are the ingredients of the pill. Concentration gradients from 1000 µg/ml to 0.02 µg/ml were prepared using egg albumin and phosphate buffer saline. The pill was incubated in controlled experimental settings at various concentrations, and the absorbance was measured to quantify the level of albumin denaturation. One NSAID (Ibuprofen) and one steroid (Prednisolone) were used as the reference drugs. The percentage inhibition of protein denaturation at each concentration was calculated by using the formula of (Vt / Vc - 1) x 100% where Vt = absorbance of the test sample and Vc = absorbance of control.
Results: The maximum inhibition rate of egg albumin denaturation (46.7%) was seen in the 200 µg/ml concentration, while Prednisolone and Ibuprofen had 2.5% and 24.6% inhibition rates in the same concentration, respectively. Less than 35% inhibition rate of egg albumin denaturation showed in all other concentrations lower than 200 µg/ml (0.1 µg/ml – 100 µg/ml) of this STMP. The inhibition rates of these concentrations did not differ noticeably from those of the reference medications. In addition, the STMP had a 28.3% inhibited rate of egg albumin denaturation in 1000 µg/ml concentration while Prednisolone and Ibuprofen showed low inhibition rates compared to the STMP (3.4% and 13.8% respectively) in this concentration.
Conclusion: This study discovered that 200 µg/ml of the tested Sri Lankan Traditional Medicine pill had the strongest anti-inflammatory efficacy against protein denaturation. Furthermore, the effect against albumin denaturation in high-concentration Sri Lankan STMP (200 µg/ml and 1000 µg/ml) surpassed that of the reference drugs, signifying a more pronounced impact.
{"title":"Evaluation of In-vitro Anti-inflammatory Activity of Sri Lankan Medicinal Pill of Aloe vera, Centella asiatica, and Strychnos potatorum Exacts Using Egg Albumin Denaturation Assay","authors":"G. D. S. Pushpika, R. S. Maddumage, H. E. H. Perera, R. S. P. Fernando, A. R. N. Silva","doi":"10.9734/aprj/2023/v11i6227","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i6227","url":null,"abstract":"Aims: The study aims to evaluate the albumin heat denaturalization inhibition activity of a Sri Lankan Traditional Medicine pill (STMP) as an indirect measure of anti-inflammation.
 Methodology: The extracts of Aloe vera, Centella asiatica (Asiatic pennywort), and Strychnos potatorum (Cleaning nut) are the ingredients of the pill. Concentration gradients from 1000 µg/ml to 0.02 µg/ml were prepared using egg albumin and phosphate buffer saline. The pill was incubated in controlled experimental settings at various concentrations, and the absorbance was measured to quantify the level of albumin denaturation. One NSAID (Ibuprofen) and one steroid (Prednisolone) were used as the reference drugs. The percentage inhibition of protein denaturation at each concentration was calculated by using the formula of (Vt / Vc - 1) x 100% where Vt = absorbance of the test sample and Vc = absorbance of control.
 Results: The maximum inhibition rate of egg albumin denaturation (46.7%) was seen in the 200 µg/ml concentration, while Prednisolone and Ibuprofen had 2.5% and 24.6% inhibition rates in the same concentration, respectively. Less than 35% inhibition rate of egg albumin denaturation showed in all other concentrations lower than 200 µg/ml (0.1 µg/ml – 100 µg/ml) of this STMP. The inhibition rates of these concentrations did not differ noticeably from those of the reference medications. In addition, the STMP had a 28.3% inhibited rate of egg albumin denaturation in 1000 µg/ml concentration while Prednisolone and Ibuprofen showed low inhibition rates compared to the STMP (3.4% and 13.8% respectively) in this concentration.
 Conclusion: This study discovered that 200 µg/ml of the tested Sri Lankan Traditional Medicine pill had the strongest anti-inflammatory efficacy against protein denaturation. Furthermore, the effect against albumin denaturation in high-concentration Sri Lankan STMP (200 µg/ml and 1000 µg/ml) surpassed that of the reference drugs, signifying a more pronounced impact.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135215973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-17DOI: 10.9734/aprj/2023/v11i6225
Md. Rabbi Hasan, A. K. M. Zakir Hossain, Muhammad Humayun Kabir, Md. Mohimenul Islam, S. M. Abdul Alim, Md. Robiul Islam Akondo
A hydroponic pot experiment was driven to investigate the improvement of drought tolerance in six maize varieties (e.g. Gold Star, BHM 14, Paloan, Bharti 981, BHM 9, Pioneer) based on morpho-physiological and biochemical characteristics. Six Maize varieties were used as test crops and the experiment was laid out in a completely randomized design (RCBD) with three replications. Drought stress was imposed 14 days after sowing by using the PEG solution. The results revealed that BHM 14 possessed the longest root length (69.33 cm), higher shoot length (42.67 cm), longest total plant height (112.00 cm), highest fresh root weight (3.32 g), high leaf greenness found in BHM 14 measured by SPAD meter in both top and lower leaves, high amount of proline whereas, Paloan produced shortest total plant height (78.50 cm), lowest fresh root weight (1.52 g), lowest proline after 42 days of sowing under stress condition. The maximal photochemical efficiency (Fv/Fm) appeared to measure photosynthesis whereas Pioneer was comparatively higher in stress conditions. The fresh weight of root and shoot was significantly least affected in stress conditions, whereas BHM 14 performed better in stress conditions. On the other hand, varieties had a significant difference in total dry matter of control condition and stress condition. Therefore, it is suggested that BHM 14 showed maximum drought tolerance in respect of growth and morpho-physiological changes under drought conditions.
{"title":"Evaluation of Maize Varieties at Seedling Stage under Drought Stress Based on Morpho-physiological and Biochemical Attributes","authors":"Md. Rabbi Hasan, A. K. M. Zakir Hossain, Muhammad Humayun Kabir, Md. Mohimenul Islam, S. M. Abdul Alim, Md. Robiul Islam Akondo","doi":"10.9734/aprj/2023/v11i6225","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i6225","url":null,"abstract":"A hydroponic pot experiment was driven to investigate the improvement of drought tolerance in six maize varieties (e.g. Gold Star, BHM 14, Paloan, Bharti 981, BHM 9, Pioneer) based on morpho-physiological and biochemical characteristics. Six Maize varieties were used as test crops and the experiment was laid out in a completely randomized design (RCBD) with three replications. Drought stress was imposed 14 days after sowing by using the PEG solution. The results revealed that BHM 14 possessed the longest root length (69.33 cm), higher shoot length (42.67 cm), longest total plant height (112.00 cm), highest fresh root weight (3.32 g), high leaf greenness found in BHM 14 measured by SPAD meter in both top and lower leaves, high amount of proline whereas, Paloan produced shortest total plant height (78.50 cm), lowest fresh root weight (1.52 g), lowest proline after 42 days of sowing under stress condition. The maximal photochemical efficiency (Fv/Fm) appeared to measure photosynthesis whereas Pioneer was comparatively higher in stress conditions. The fresh weight of root and shoot was significantly least affected in stress conditions, whereas BHM 14 performed better in stress conditions. On the other hand, varieties had a significant difference in total dry matter of control condition and stress condition. Therefore, it is suggested that BHM 14 showed maximum drought tolerance in respect of growth and morpho-physiological changes under drought conditions.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135993102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-16DOI: 10.9734/aprj/2023/v11i6226
Sanjay Singh, Rajiv Pandey, Rameshwar Das
Diospyros melanoxylon Roxb. leaves used in manufacture of indigenous traditional cigarette, called as bidi, contributes to social economic livelihood of rural and tribal people in India generating a source of subsidiary occupation and supplementary income apart from providing significant revenue to state forest departments. However, a reliable, scientific and statistically sound estimate of its production is essential to obtain optimal revenue and livelihood opportunities in the sector. Thus, the present study was carried out to quantify production of D. melanoxylon leaves in the state of Jharkhand, India on a scientific basis. Collection of D. melanoxylon leaves being a time-bound seasonal activity, field survey and data collection was planned by dividing the state into in five administrative zones namely Palamu, Hazaribagh, Giridih, Singhbhum and Ranchi divided into 45 MFP ranges comprising of 295 lots and 686 collection units. The focus of sampling was estimation of number of plants in a fixed area i.e. plant density, number of pluck able leaves per plant, number of total pluckable leaves per plant, and plant growth geometry. A novel sampling strategy designed as Stratified Cluster Line Transect Quadrat Random Sampling comprising of essential elements of sampling strategy and vegetational survey methods with line transect of 100m x 10m and quadrate of 5m x 5m. In all, 52% collection units from all lots of entire MFP ranges were surveyed. Three permanent plots per MFP range were also maintained to evaluate the quantum of leaf harvesting throughout the collection season by collecting all pluckable leaves. The productivity of D. melanoxylon leaves was found around 11.50 lakh standard bags (52000 tonnes). However, realized yield in absence of pruning/pollarding and other silvicultural operation as well as the political disturbance severely hampering efficient working, may be less.
{"title":"Estimation of Diospyros melanoxylon Roxb. Leaves Production in Forests of Jharkhand, India","authors":"Sanjay Singh, Rajiv Pandey, Rameshwar Das","doi":"10.9734/aprj/2023/v11i6226","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i6226","url":null,"abstract":"Diospyros melanoxylon Roxb. leaves used in manufacture of indigenous traditional cigarette, called as bidi, contributes to social economic livelihood of rural and tribal people in India generating a source of subsidiary occupation and supplementary income apart from providing significant revenue to state forest departments. However, a reliable, scientific and statistically sound estimate of its production is essential to obtain optimal revenue and livelihood opportunities in the sector. Thus, the present study was carried out to quantify production of D. melanoxylon leaves in the state of Jharkhand, India on a scientific basis. Collection of D. melanoxylon leaves being a time-bound seasonal activity, field survey and data collection was planned by dividing the state into in five administrative zones namely Palamu, Hazaribagh, Giridih, Singhbhum and Ranchi divided into 45 MFP ranges comprising of 295 lots and 686 collection units. The focus of sampling was estimation of number of plants in a fixed area i.e. plant density, number of pluck able leaves per plant, number of total pluckable leaves per plant, and plant growth geometry. A novel sampling strategy designed as Stratified Cluster Line Transect Quadrat Random Sampling comprising of essential elements of sampling strategy and vegetational survey methods with line transect of 100m x 10m and quadrate of 5m x 5m. In all, 52% collection units from all lots of entire MFP ranges were surveyed. Three permanent plots per MFP range were also maintained to evaluate the quantum of leaf harvesting throughout the collection season by collecting all pluckable leaves. The productivity of D. melanoxylon leaves was found around 11.50 lakh standard bags (52000 tonnes). However, realized yield in absence of pruning/pollarding and other silvicultural operation as well as the political disturbance severely hampering efficient working, may be less.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136113010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.9734/aprj/2023/v11i5224
Theophilus Kabiru, Peter K. Njenga
Background and Objective: Lagenaria siceraria is extensively grown as a vegetable crop in tropical and subtropical regions of the world as a source of food and for it’s medicinal potential. The purpose of this research was to determine the optimal conditions for plantlet regeneration of Lagenaria siceraria microshoots on various concentrations of benzyl amino purine and naphthalene acetic acid in vitro.
Materials and Methods: The research was designed as a completely randomized design. Shoot tips of Lagenaria siceraria were surface sterilized with sodium hypochlorite(NaOCl) at different concentrations (0,3,4,5,6 and 7 mg/L) and cultured on Murashige and Skoog (MS) solidified medium supplemented with 6-benzylaminopurine (BAP) at different concentrations of (0,0.5,1.0,1.5,2.0,2.5 and 3.0mg/L) for shoot induction and the obtained shoots were transferred to MS medium containing naphthaleneacetic Acid(NAA) at different concentrations (0,0.5,1.0,1.5,2.0,2.5 AND 3.0mg/L) for root induction.
Results: Surface disinfection was optimal after immersion of shoot tips and/or nodal explants in cleaning detergent (5 min), ethanol 70% (1 min), and sodium hypochlorite 5mg/L (15 min). MS medium supplemented with 2.0 mg/L BAP was most effective in inducing shoot multiplication (100%), with an average of 4,33 shoots per explant after 4 weeks. MS medium supplemented with 2.0 mg/L NAA was most appropriate for rooting (100%) with an average of 5.33 roots per shoot after 4 weeks.
Conclusion: shoots sterilized with 5mg/L NaOCl and inoculated in MS medium supplemented with 2.0 mg/LBAP and 2.0 mg/L NAA were optimal conditions for the surface sterilization, shoot and root induction of Lagenaria siceraria shoots.
{"title":"In vitro Propagation of Lagenaria Siceraria: A Plant of the Cucurbitaceae Family with High Medicinal Value","authors":"Theophilus Kabiru, Peter K. Njenga","doi":"10.9734/aprj/2023/v11i5224","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i5224","url":null,"abstract":"Background and Objective: Lagenaria siceraria is extensively grown as a vegetable crop in tropical and subtropical regions of the world as a source of food and for it’s medicinal potential. The purpose of this research was to determine the optimal conditions for plantlet regeneration of Lagenaria siceraria microshoots on various concentrations of benzyl amino purine and naphthalene acetic acid in vitro.
 Materials and Methods: The research was designed as a completely randomized design. Shoot tips of Lagenaria siceraria were surface sterilized with sodium hypochlorite(NaOCl) at different concentrations (0,3,4,5,6 and 7 mg/L) and cultured on Murashige and Skoog (MS) solidified medium supplemented with 6-benzylaminopurine (BAP) at different concentrations of (0,0.5,1.0,1.5,2.0,2.5 and 3.0mg/L) for shoot induction and the obtained shoots were transferred to MS medium containing naphthaleneacetic Acid(NAA) at different concentrations (0,0.5,1.0,1.5,2.0,2.5 AND 3.0mg/L) for root induction.
 Results: Surface disinfection was optimal after immersion of shoot tips and/or nodal explants in cleaning detergent (5 min), ethanol 70% (1 min), and sodium hypochlorite 5mg/L (15 min). MS medium supplemented with 2.0 mg/L BAP was most effective in inducing shoot multiplication (100%), with an average of 4,33 shoots per explant after 4 weeks. MS medium supplemented with 2.0 mg/L NAA was most appropriate for rooting (100%) with an average of 5.33 roots per shoot after 4 weeks.
 Conclusion: shoots sterilized with 5mg/L NaOCl and inoculated in MS medium supplemented with 2.0 mg/LBAP and 2.0 mg/L NAA were optimal conditions for the surface sterilization, shoot and root induction of Lagenaria siceraria shoots.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-20DOI: 10.9734/aprj/2023/v11i5223
Oyeyemi. S. Dele, None Raymond O.
Corn silk is an agricultural waste material from corn cultivation. The need for a supplement feed material that would be readily available, affordable and has nutritional qualities prompted this study. Fresh and dry corn silk were collected from a farm land in Ado Ekiti, Ekiti State, Nigeria and evaluated for phytochemical and nutritional composition using standard techniques. The phytochemical analysis revealed that both the young and matured corn silk contained alkaloids, tannins, saponins, flavonoids, phenols, steroids and reducing sugar. The qualitative phytochemical estimates showed high concentration of flavonoids and total phenols while tannins were found in moderate quantities. The proximate estimation (%) revealed high values of carbohydrate and crude fiber, moderate crude protein and ash, low crude fat, low moisture as well as high energy content in young and matured corn silk respectively. Both samples contained high K, P, Ca, Mg, moderate Na, Fe, Mn, Zn and Cu while Pb and Ni, toxic metals, were absent. The results showed low mineral CV% which ranged from 1.32-38.92. The results of the mineral safety index were lower than the standard, an indication that there would be no mineral overload. The two corn silk samples were rich in active secondary metabolites which could offer protective health benefits, also safe and contained nutritional qualities that could make them suitable to be incorporated into man’s diet and domestic feed.
{"title":"Phytochemical and Nutritional Profile, Mineral Ratio and Mineral Safety Index of Young and Matured Corn Silk Collected from Ekiti State, Nigeria","authors":"Oyeyemi. S. Dele, None Raymond O.","doi":"10.9734/aprj/2023/v11i5223","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i5223","url":null,"abstract":"Corn silk is an agricultural waste material from corn cultivation. The need for a supplement feed material that would be readily available, affordable and has nutritional qualities prompted this study. Fresh and dry corn silk were collected from a farm land in Ado Ekiti, Ekiti State, Nigeria and evaluated for phytochemical and nutritional composition using standard techniques. The phytochemical analysis revealed that both the young and matured corn silk contained alkaloids, tannins, saponins, flavonoids, phenols, steroids and reducing sugar. The qualitative phytochemical estimates showed high concentration of flavonoids and total phenols while tannins were found in moderate quantities. The proximate estimation (%) revealed high values of carbohydrate and crude fiber, moderate crude protein and ash, low crude fat, low moisture as well as high energy content in young and matured corn silk respectively. Both samples contained high K, P, Ca, Mg, moderate Na, Fe, Mn, Zn and Cu while Pb and Ni, toxic metals, were absent. The results showed low mineral CV% which ranged from 1.32-38.92. The results of the mineral safety index were lower than the standard, an indication that there would be no mineral overload. The two corn silk samples were rich in active secondary metabolites which could offer protective health benefits, also safe and contained nutritional qualities that could make them suitable to be incorporated into man’s diet and domestic feed.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136308952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-14DOI: 10.9734/aprj/2023/v11i5222
Shabnam Kumari Thakur, None N. V. L. Suvarchala Reddy V., None Ganga Raju M., Poojeetha Sree, Sappa Sowmya, None Sree Lakshmi S., None Suchitra J.
The current study focuses on the green production of silver nanoparticles utilising an ethanolic extract of Millingtonia hortensis leaves. This medicinal plant was high in the phenol and flavonoids classes of chemicals. They converted silver nitrate into silver nanoparticles (AgNPs), which were then characterised by FT-IR, particle size analysis, and Zeta potential. The FT-IR analysis confirmed the existence of several functional groups around AgNPs. The particle size examination revealed that the synthesised silver nanoparticles were spherical in shape and nano in size. The zeta potential of AgNPs was determined to be -14 mV; this negative value validates particle repulsion and so increases the formulation's stability. The antioxidant and anti-diabetic properties of AgNPs were investigated, and the results revealed significant free radical scavenging ability, inhibition of carbohydrate digesting enzymes (α-Amylase and α-glucosidase). The green synthesized silver nanoparticles were found to be a promising phyto-medicine for the treatment of diabetes.
{"title":"Green Synthesis and Characterization of Silver Nanoparticles from Millingtonia hortensis and Their Antidiabetic and Antioxidant Efficacy","authors":"Shabnam Kumari Thakur, None N. V. L. Suvarchala Reddy V., None Ganga Raju M., Poojeetha Sree, Sappa Sowmya, None Sree Lakshmi S., None Suchitra J.","doi":"10.9734/aprj/2023/v11i5222","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i5222","url":null,"abstract":"The current study focuses on the green production of silver nanoparticles utilising an ethanolic extract of Millingtonia hortensis leaves. This medicinal plant was high in the phenol and flavonoids classes of chemicals. They converted silver nitrate into silver nanoparticles (AgNPs), which were then characterised by FT-IR, particle size analysis, and Zeta potential. The FT-IR analysis confirmed the existence of several functional groups around AgNPs. The particle size examination revealed that the synthesised silver nanoparticles were spherical in shape and nano in size. The zeta potential of AgNPs was determined to be -14 mV; this negative value validates particle repulsion and so increases the formulation's stability. The antioxidant and anti-diabetic properties of AgNPs were investigated, and the results revealed significant free radical scavenging ability, inhibition of carbohydrate digesting enzymes (α-Amylase and α-glucosidase). The green synthesized silver nanoparticles were found to be a promising phyto-medicine for the treatment of diabetes.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134913493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.9734/aprj/2023/v11i5221
Tao Yuan, Xin-yu Wen, Zhi-qiang Zhang, Yi Huang
Objective: Compared the nuclear magnetic resonance (NMR) fingerprint spectra of Ophiopogon japonicus from three planted regions in Sichuan province and used multiple stoichiometric methods to distinguish these medicinal material's production areas. Methods: The 1H-NMR data of three Ophiopogon japonicus-producing areas in Sichuan province were obtained. Three stoichiometric methods---similarity analysis, clustering analysis, and principal component analysis (PCA) were used to analyze these 1H-NMR characteristics. Results: There were significant differences in Ophiopagon japonicus from the three production areas. Samples in distinct regions of the same large production area could also observe a slight divergence. The similarity analysis, clustering analysis, and PCA analysis results consistently indicated that NMR spectroscopy combined with chemical measurement analysis could identify the production areas of medicinal material. Conclusion: This study established a method of determining the Ophiopogon japonicus's district based on the 1H-NMR fingerprint map and stoichiometric methods. The technique was relatively simple and could serve as a basis for identifying the production areas of Ophiopogon japonicus, providing a reference for developing and utilization of Ophiopogon japonicus.
{"title":"Production Area Discrimination of Ophiopogon japonicus Using 1H-NMR Fingerprints and Stoichiometric Method","authors":"Tao Yuan, Xin-yu Wen, Zhi-qiang Zhang, Yi Huang","doi":"10.9734/aprj/2023/v11i5221","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i5221","url":null,"abstract":"Objective: Compared the nuclear magnetic resonance (NMR) fingerprint spectra of Ophiopogon japonicus from three planted regions in Sichuan province and used multiple stoichiometric methods to distinguish these medicinal material's production areas. \u0000Methods: The 1H-NMR data of three Ophiopogon japonicus-producing areas in Sichuan province were obtained. Three stoichiometric methods---similarity analysis, clustering analysis, and principal component analysis (PCA) were used to analyze these 1H-NMR characteristics. \u0000Results: There were significant differences in Ophiopagon japonicus from the three production areas. Samples in distinct regions of the same large production area could also observe a slight divergence. The similarity analysis, clustering analysis, and PCA analysis results consistently indicated that NMR spectroscopy combined with chemical measurement analysis could identify the production areas of medicinal material. \u0000Conclusion: This study established a method of determining the Ophiopogon japonicus's district based on the 1H-NMR fingerprint map and stoichiometric methods. The technique was relatively simple and could serve as a basis for identifying the production areas of Ophiopogon japonicus, providing a reference for developing and utilization of Ophiopogon japonicus.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86037232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-08DOI: 10.9734/aprj/2023/v11i5220
Safoora Borzoo, S. Mohsenzadeh, D. Kahrizi
Camelina sativa, being a flowering plant in the family Brassicaceae, is traditionally cultivated as an oilseed crop due to its exceptionally high level (up to 45%) of omega-3 fatty acids, which is uncommon in vegetable sources. In the recent years camelina has been growing more than before because of its potentiality in the production of biodiesel and bioproduct. An aforementioned experiment was designed in three irrigation levels (100%, 75% and 50% of field capacity) and Micrococcus yunnanensis was used as plant growth-promoting bacteria during the reproductive phase. The bacteria were incubated in a nutrient broth medium at the temperature of 28°C for 24h on shaker ceaselessly. When sowing, seeds were treated with 1.0 ml bacteria suspension in the inoculated groups. Water stress was applied to each pot in the levels of 75 and 50 % FC from the budding stage to full maturity in generative phase. Camelina silique yield was harvested at the maturity stage for analysis of seed quality and biochemical responses. Total soluble carbohydrate was extracted thrice from 100 mg of mature seed using extraction soluble including glacial acetic acid, methanol, and water. Oil and protein content were measured using Near-Infrared Reflectance spectrometer. Seed fatty acid contents were determined using gas chromatography. The results showed a significant relation between the highest proportion of fatty acid and the polyunsaturated fatty acid (55.12 to 65.66%) in particular linolenic acid. The increase of polyunsaturated fatty acid and saturated fatty acid was coincided with the decrease of monounsaturated fatty acid under water deficit stress. The application of plant growth-promoting bacteria is proven to increase protein with 50% of field capacity. In general, water deficit stress and plant growth-promoting bacteria have significant effects on the remobilization of nutrients from the soil to developing seed and following metabolism synthesis.
{"title":"Influences of Water Deficit Stress and Symbiosis with Growth-Promoting Bacteria on Seed Biochemistry of Camelina sativa","authors":"Safoora Borzoo, S. Mohsenzadeh, D. Kahrizi","doi":"10.9734/aprj/2023/v11i5220","DOIUrl":"https://doi.org/10.9734/aprj/2023/v11i5220","url":null,"abstract":"Camelina sativa, being a flowering plant in the family Brassicaceae, is traditionally cultivated as an oilseed crop due to its exceptionally high level (up to 45%) of omega-3 fatty acids, which is uncommon in vegetable sources. In the recent years camelina has been growing more than before because of its potentiality in the production of biodiesel and bioproduct. An aforementioned experiment was designed in three irrigation levels (100%, 75% and 50% of field capacity) and Micrococcus yunnanensis was used as plant growth-promoting bacteria during the reproductive phase. \u0000The bacteria were incubated in a nutrient broth medium at the temperature of 28°C for 24h on shaker ceaselessly. When sowing, seeds were treated with 1.0 ml bacteria suspension in the inoculated groups. Water stress was applied to each pot in the levels of 75 and 50 % FC from the budding stage to full maturity in generative phase. Camelina silique yield was harvested at the maturity stage for analysis of seed quality and biochemical responses. Total soluble carbohydrate was extracted thrice from 100 mg of mature seed using extraction soluble including glacial acetic acid, methanol, and water. Oil and protein content were measured using Near-Infrared Reflectance spectrometer. Seed fatty acid contents were determined using gas chromatography. The results showed a significant relation between the highest proportion of fatty acid and the polyunsaturated fatty acid (55.12 to 65.66%) in particular linolenic acid. The increase of polyunsaturated fatty acid and saturated fatty acid was coincided with the decrease of monounsaturated fatty acid under water deficit stress. The application of plant growth-promoting bacteria is proven to increase protein with 50% of field capacity. In general, water deficit stress and plant growth-promoting bacteria have significant effects on the remobilization of nutrients from the soil to developing seed and following metabolism synthesis.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84875761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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