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Efficacy of Some Biorational Approaches against Two-Spotted Spider Mite (Tetranychus urticae Koch) in Bitter Gourd (Momordica charantia L.) 几种生物防治苦瓜双斑蜘蛛螨的效果研究
Pub Date : 2022-05-09 DOI: 10.9734/aprj/2022/v9i230202
M. M. Rahman, S. Akter, M. Hossain, M. Hossain
Aims: The aim of the study was to test the effectiveness of several biorational techniques against two-spotted spider mite, Tetranychus urticae Koch. in bitter gourd. Study Design: The study used a single component Randomized Complete Block Design (RCBD) with three replications. Place and Duration of Study: The experiment was conducted at Sher-e-Bangla Agricultural University's central farm in Dhaka, Bangladesh from March 2021 to July 2021. Methodology: The study included seven treatments. T1= Spinomax (0.4ml/L water); T2= Neem Oil (5ml/L water); T3= Biomax (1.2ml/L water); T4= Ripcord (1ml/L water); T5= Trichoderma spp (200mg/L water); T6= Mehogany Leaf Extract (200mg/L water); T7= Absolute (Control). All the treatments were applied at 15 days interval. Results: Treatments for mite infestations showed varying responses in experiments with various biorational control techniques. Trichoderma spp. had the highest results, followed by neem oil, which was effective against mite pests infesting bitter gourd. The results showed that when Trichoderma spp. was used to treat leaf infestation, the total number of fruit set, % healthy fruit, and % infested fruit were all greater. Ripcord had the lowest outcomes against mite pests in most circumstances, with the exception of control treatment. Conclusion: It can be concluded that Trichoderma spp. @200mg/L of water can be used to treat two spotted spider mites in an environmentally acceptable manner.
目的:研究几种生物防螨技术对荨麻疹叶螨的防螨效果。在苦瓜里。研究设计:本研究采用单组分随机完全区设计(RCBD),有3个重复。研究地点和时间:实验于2021年3月至2021年7月在孟加拉国达卡的Sher-e-Bangla农业大学中央农场进行。方法:本研究包括7种治疗方法。T1= Spinomax (0.4ml/L水);T2=印楝油(5ml/L水);T3= Biomax (1.2ml/L水);T4=拉索(1ml/L水);T5=木霉spp (200mg/L水);T6=桃花心木叶提取物(200mg/L水);T7=绝对(控制)。所有处理间隔15 d。结果:不同生物防治技术对螨害的防治效果不同。其中木霉效果最好,印楝油效果次之,对苦瓜的螨虫有较好的防治效果。结果表明,用木霉处理叶片侵染时,总坐果数、健康果率和侵染果率均较大;在大多数情况下,除对照处理外,开伞索对螨虫的防治效果最低。结论:用@200mg/L的水处理木霉对2只斑蛛螨可达到环保可接受的效果。
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引用次数: 0
Effect of Photoperiod and Nutritional-Shock on Increasing the Number of Minitubers from Apical Rooted Cuttings Grown in Coco-Peat 光周期和营养冲击对增加椰泥根茎扦插苗数的影响
Pub Date : 2022-05-05 DOI: 10.9734/aprj/2022/v9i230201
Mai Hải Châu, Nguyen The Nhuan
Currently, a variety of techniques for producing potato minituber have been investigated, including soilless aeroponic, hydroponic and deep-water culture systems. However, minitubers potato production in the greenhouse from apical rooted cutting grown coco-peat under a photoperiod and nutritional - shock system is a new and creative idea. Therefore, the objective of the study was to optimize the effect of photoperiod and nutritional-shock on growth performance and yield of first generation potato tuber production of potato plants. Potato variety ‘Atlantic’ was treated under 5 photoperiod – (8 h/day, 9 h/day, 10 h/day, 11 h/day and natural light) under nutritional-shock process with reducing N concentration in nutrient solution (0;25%; 50%;75% and 100%), the growth stage of plant to conduct nutritional-shock (30, 35, 40, 45 and 50 DAT), the implementation time of nutritional-shock (12, 24, 48 and 60 hours) and the combination of photoperiod (9 h/day, 10 h/day) and nutritional-shock (75%, 100%) on the formation and development of minitubers. The study revealed that photoperiod at 10h/day showed the best result with 7.4 tubers/ plant and 569.7 tubers m-2. The number of tuber stolons, average number of tubers and tuber yield was directly proportional to the decreasing in N concentration in the nutrient solution, in which the highest was the 100% reduction in N concentration with 9.3 tubers/plant, higher than the control treatment 300%. The nutrient shock treatment at 30 DAT in 48 ours gave the best tuber production efficiency. There was a strong interaction between photoperiod and nutritional-shock on the formation and yield of potatoes (10.6 tubers/plant, 308 tubers m-2).
目前,人们研究了多种马铃薯微型栽培技术,包括无土气培、水培和深水栽培。然而,在光周期和营养冲击系统下,利用根茎扦插种植的可可豆在温室内生产小块薯是一种新的、有创造性的想法。因此,本研究的目的是优化光周期和营养休克对第一代马铃薯块茎植株生长性能和产量的影响。采用5个光周期(8 h/d、9 h/d、10 h/d、11 h/d和自然光)处理马铃薯品种“大西洋”,降低营养液中N浓度(0;25%;50%;75%和100%),植物进行营养休克的生长阶段(30、35、40、45和50个DAT),营养休克的实施时间(12、24、48和60小时)以及光周期(9 h/天、10 h/天)和营养休克(75%、100%)对微孢子形成和发育的影响。结果表明,光周期为10h/d时,植株产量为7.4根/株,m-2产量为569.7根。块茎匍匐茎数、平均块茎数和块茎产量与营养液中氮浓度的降低成正比,其中氮浓度降幅最大,达到100%,为9.3根/株,比对照处理高出300%。营养休克处理在30dat, 48h后块茎生产效率最高。光周期和营养休克对马铃薯(10.6根/株,308根m-2)的形成和产量有较强的交互作用。
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引用次数: 0
In vitro Assessment of Pseudomonas sp. Strain FCBB-2 for Effective Plant Growth Promotion and Antifungal Activity under Drought Stress 假单胞菌菌株FCBB-2在干旱胁迫下促进植物生长和抗真菌活性的体外研究
Pub Date : 2022-02-10 DOI: 10.9734/aprj/2022/v9i230200
Sai Shiva Krishna Prasad Vurukonda, Sandhya Vardharajula, Ali Shaik Zulfikar
The significance of plant growth-promoting rhizobacteria (PGPR) in plant adaptation to harsh conditions is still unknown. Native bacteria were isolated from rhizospheric soils and tested for growth-promoting qualities as well as antagonistic capabilities against phytopathogenic fungi in this investigation. All the Pseudomonas spp. strains were characterized for in vitro drought tolerance in trypticase soy broth supplemented with different concentrations of polyethylene glycol (PEG6000). Out of nine isolates, only one strain was able to tolerate maximum level of stress (−1.03 MPa) and is further screened for plant growth promoting (PGP) properties under non-stress and drought stress conditions. Strain FCBB-2 isolated from cluster bean plant rhizosphere soil, showed multiple PGP activities such as indole-3-acetic acid (IAA) production, siderophore, hydrogen cyanide (HCN) production and exopolysaccharide (EPS) production and antifungal activity under non-stress and drought stress conditions against various plant fungal pathogens like Fusarium oxysporum, Macrophomina phaseolina, Alternaria alternata, Sclerotium hydrophilium, Pythium aphanidermatum and Rhizoctonia solani. The strain FCBB-2 was identified as Pseudomonas aeruginosa based on morphological and 16S rRNA gene sequence analysis and the sequence was submitted to NCBI GenBank under the accession number KT311003.
植物促生根瘤菌(PGPR)在植物适应恶劣环境中的作用尚不清楚。本研究从根际土壤中分离出原生细菌,并测试了其促进生长的特性和对植物病原真菌的拮抗能力。所有假单胞菌菌株对添加不同浓度聚乙二醇(PEG6000)的胰酶豆汤具有体外耐旱性。在9个分离株中,只有1株能够耐受最大水平的胁迫(- 1.03 MPa),并在非胁迫和干旱胁迫条件下进一步筛选植物生长促进(PGP)特性。菌株FCBB-2从丛豆植物根际土壤中分离得到,在非胁迫和干旱胁迫条件下对尖孢镰刀菌(Fusarium oxysporum)、phaseolina、alternnaria alternata、Sclerotium hydroophilum、pyium aphanidermatum和Rhizoctonia solani等多种植物真菌病原菌具有吲哚-3-乙酸(IAA)、铁载体(siderophina)、氰化氢(HCN)和胞外多糖(EPS)等多种PGP活性和抗真菌活性。菌株FCBB-2经形态学和16S rRNA基因序列分析鉴定为铜绿假单胞菌(Pseudomonas aeruginosa),并将序列提交至NCBI GenBank,登录号为KT311003。
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引用次数: 0
Diversity of Homestead Plants in Sreemangal Upazilla, Bangladesh 孟加拉国Sreemangal Upazilla地区家园植物的多样性
Pub Date : 2022-02-05 DOI: 10.9734/aprj/2022/v9i230199
Sabiha Akter Seema, Joyanta Banik, Rajassree Nandi
The research was carried out in the Kalighat union of Sreemangal Upazilla, Moulvibazar district, Bangladesh with the objective of discovering the diversity of homestead plants. Five villages were selected randomly from the Kalighat union named Kalighat tea garden, Baraura Tea Garden, 8 No. Line, 8 No Kalighat and 9 No. Basti. To determine the plant diversity, 105 households were surveyed. In comparison to the other villages, the 8 No. Line had more plant diversity, in this study. In the study region, 73 valuable plant species were identified, including fruits, woods, fuel wood, aesthetics, and medicinal. The highest Shannon Wiener Index value was found as 2.95 in 8 No. Line and the lowest Shannon Wiener Index value was 2.21 in Kalighat Tea Garden. On the other hand, Baraura Tea Garden had the highest Index of Dominance (0.47), while 8 No. Kalighat and 8 No. Line had the lowest Index of Dominance (0.09). The Evenness Index was found in the plot of Kalighat tea garden (0.76), Baraura tea garden (0.7) and 8 No. Line (0.77) were more evenly distributed except 8 No. Kalighat (0.84) and 9 No. Basti (0.64) indicated irregular even distribution. Plant species diversity among different villages of Sreemangal Upazilla was found statistically significant (p-value =0.0001173<0.05). The most important value index and top-ranked planted species were Bamboo (Bambusa sp.) (42.20) followed by Mango (Mangifera indica) (37.79), Kathal (Artocarpus heterophyllus) (15.19) and Supari (Areca catechu) (35.88) respectively. Agro-crop diversity was also found to be predominant in different unions of Sreemangal Upazilla. A total of 24 agro-crop species were found in the study area. Rice crop was found the most important crop in the study area. Rice production in each family is 1200 kilograms per year, whereas consumption is 500.5 kilograms per year. Farmers can be authorized more specific species for more production and sustainable homestead forest management in the study area based on findings from the analysis of soil conditions and properties. The present study revealed that homestead plant diversity and homestead crops are the prime resources for the socio-economic development of rural people.
这项研究是在孟加拉国Moulvibazar地区Sreemangal Upazilla的Kalighat联盟进行的,目的是发现家园植物的多样性。从卡利加特联盟中随机抽取了5个村庄,命名为卡里加特茶园,Baraura茶园,8号。8号线和9号线。Basti。为确定植物多样性,对105户家庭进行了调查。与其他村庄相比,8号村的情况更差。在本研究中,Line具有更多的植物多样性。在研究区共鉴定出有价值的植物73种,包括水果、木材、薪柴、美学和药用等。Shannon Wiener指数最高值为2.95。卡里加特茶园Shannon Wiener指数最低值为2.21。优势度指数最高的是Baraura茶园,为0.47;Kalighat和8号。株系的显性指数最低,为0.09。均匀度指数在卡列加特茶园(0.76)、巴罗拉茶园(0.7)和8号茶园均有显著差异。除8号线外,其余线(0.77)分布均匀。Kalighat(0.84)和9位。Basti(0.64)表示不规则均匀分布。Sreemangal Upazilla不同村落间植物物种多样性差异有统计学意义(p值=0.0001173<0.05)。最重要的价值指数和排名最高的种植树种为竹(Bambusa sp.)(42.20),其次为芒果(Mangifera indica)(37.79)、Kathal (Artocarpus heterophyllus)(15.19)和Supari (Areca catechu)(35.88)。不同组合的作物多样性也具有优势。研究区共发现24种农作物。水稻是研究区最重要的作物。每户每年的大米产量为1200公斤,而消费量为500.5公斤。根据对土壤条件和性质的分析结果,可以授权农民在研究地区种植更具体的物种,以提高产量和可持续的家园森林管理。本研究表明,耕地植物多样性和耕地作物是农村人口社会经济发展的主要资源。
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引用次数: 0
Influence of Seedling Age and Training on Yield Performance of Cucumber (Cucumis sativus) 苗龄和栽培对黄瓜产量性能的影响
Pub Date : 2022-01-30 DOI: 10.9734/aprj/2022/v9i130198
M. Sumi, T. Mostarin, K. Khatun, M. Samad, S. Akter, A. Khanom, Md. Rafiul Alam Khan, Md. Touhidujjaman
An experiment was conducted during the period of April to July 2019 at Horticulture farm of Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh to evaluate the yield performance of cucumber influenced by seedling age and training. The experiment was laid out in the Randomized Complete Block Design with three replications. Treatment as three different age of seedling i.e. S1=15 days old seedling, S2=20 days old seedling, S3=25 days old seedling; and four levels of training i.e. T0=Control, T1=Removal of all branches up to 40 cm height, T2=Removal of all branches up to 80 cm height and T3=Removal of all branches up to 120 cm height which in combination made 12 treatment combination. ‘Baromashi’ (local variety) was used in this experiment. Different seedling age and levels of training showed significant variations on most of the parameters. In case of seedling age, the maximum average fruits per plant (17.18), individual fruit weight (174.33 gm) and fruit yield (41.63 ton/ha) was recorded from S3 treatment, whereas the lowest was recorded from S1 treatment. On the other hand, the maximum average fruits per plant (15.93), individual fruit weight (160.87 gm) and fruit yield (35.97 ton/ha) was recorded from T1 treatment, while the lowest was recorded from T0 (control) treatment. In case of combined effect S3T1 produced the maximum average fruits per plant (19.45), weight of individual fruit (177.91 gm) and the highest yield (47.73 t/ha) while the lowest were (10.47, 132.40gm and 19.16 ton/ha) recorded from S1T0treatment combination respectively. The highest net income (730827 tk/ha) and benefit cost ratio (2.58) was noted from S3T1 treatment. It may therefore be concluded that the 25 days old seedling and removal of all branches up to 40 cm height showed more economical than rest of the combinations in cultivation of cucumber.
2019年4月至7月,在孟加拉国达卡Sher-e-Bangla农业大学园艺农场进行了一项试验,以评估苗龄和培训对黄瓜产量性能的影响。实验采用随机完全区组设计,3个重复。作为3个不同年龄的幼苗处理,即S1=15天龄的幼苗,S2=20天龄的幼苗,S3=25天龄的幼苗;以及四个级别的训练,即T0=控制,T1=去除所有高度不超过40厘米的树枝,T2=去除所有高度不超过80厘米的树枝,T3=去除所有高度不超过120厘米的树枝,这些组合构成12个处理组合。实验中使用的是“Baromashi”(当地品种)。不同苗龄和不同训练水平对大部分参数有显著影响。苗龄方面,S3处理单株平均果数最高(17.18个),单株果重最高(174.33 gm),单株果产量最高(41.63 t / hm2), S1处理最低。单株平均果数(15.93)、单株果重(160.87 gm)和单株产量(35.97 t / hm2)均以T1处理最高,而对照T0处理最低。在组合效应下,S3T1处理单株平均果数最高(19.45个),单株果重最高(177.91 gm),产量最高(47.73 t/ha),最低(10.47,132.40gm和19.16 t/ha)。S3T1处理的净收入最高(730827塔卡/公顷),效益成本比最高(2.58)。由此可见,在黄瓜栽培中,25日龄育苗和拔除40 cm以下分枝比其他组合更经济。
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引用次数: 0
Depiction of Genomic Regions and Genes Related with Drought Tolerance in Sorghum [Sorghum bicolor (L.) Moench] 高粱(Sorghum bicolor (L.))耐旱性相关基因组区域和基因描述Moench]
Pub Date : 2022-01-28 DOI: 10.9734/aprj/2022/v9i130196
Habtamu Demelash
The study of quantitative trait loci (QTLs) can bridge the gap between traditional breeding procedures and more efficient genetic improvements. The study of quantitative trait loci (QTLs) can bridge the gap between traditional breeding procedures and more efficient genetic improvements. In this report, we assess progress in mapping quantitative trait loci and genes associated with drought resistance in sorghum. Drought avoidance is linked to a number of morphological and physiological adaptations, including limiting water loss and increasing water absorption. The effects of physiological parameters will be investigated in order to find certain selection criteria that could be relevant in the development of drought-tolerant genotypes. Drought avoidance is linked to a number of morphological and physiological adaptation traits, including In grain sorghum, quantitative trait loci affecting green retention are an important feature for post blooming drought tolerance that have been investigated in various situations and with various genetic backgrounds. The two QTLs remain green (Stg2 and Stg4) of B35 x Tx7000 RILs were entirely matched in both populations, according to QTL analysis performed in the two mapping populations of recombinant inbred lines (RILs). Investigation done on different 44 sorghum lines confirmed that four QTL root nodal root angle discovered in the mapping population. Significant marker–trait association’s delignated within the quantitative chromosome (CI) for root angle (qRA2_5, qRA1_8 and qRA1_10) or closely allied to root angle (qRA1_5). Post-flowering drought response is expressed in sorghum when moisture stress occurs during grain development (GS-3). Three QTLs were detected for flowering time on chromosomes 2, 6, and 9, with log odds values (LOD) ranging from 2.5 to 3.3 and explained phenotypic variance (PVE) values calculated from 6 to 11%.
数量性状位点(qtl)的研究可以弥补传统育种方法与更有效的遗传改良之间的差距。数量性状位点(qtl)的研究可以弥补传统育种方法与更有效的遗传改良之间的差距。在这篇报告中,我们评估了高粱抗旱性的数量性状位点和相关基因的定位进展。避免干旱与许多形态和生理适应有关,包括限制水分流失和增加水分吸收。生理参数的影响将被研究,以找到某些选择标准,可能与耐旱基因型的发展有关。干旱避免与许多形态和生理适应性状有关,包括在谷物高粱中,影响绿色保持的数量性状位点是开花后抗旱性的一个重要特征,已经在各种情况和不同遗传背景下进行了研究。对重组自交系(RILs)的两个定位群体进行的QTL分析表明,B35 × Tx7000 RILs的两个QTL (Stg2和Stg4)保持绿色,在两个群体中完全匹配。对44个不同高粱品系的调查证实,在定位群体中发现了4个QTL根节根角。根角(qRA2_5、qRA1_8和qRA1_10)或与根角(qRA1_5)密切相关的标记性状在数量染色体(CI)内存在显著关联。高粱在籽粒发育过程中发生水分胁迫时,花后干旱响应得到表达(GS-3)。在染色体2、6和9上检测到3个与开花时间有关的qtl,其对数比值值(LOD)在2.5 ~ 3.3之间,解释表型方差(PVE)在6 ~ 11%之间。
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引用次数: 0
CRISPR-Cas Mediated Genome Editing: A Paradigm Shift towards Sustainable Agriculture and Biotechnology CRISPR-Cas介导的基因组编辑:向可持续农业和生物技术的范式转变
Pub Date : 2022-01-28 DOI: 10.9734/aprj/2022/v9i130197
Rao Saad Rehman, Syed Ali Zafar, Mujahid Ali, A. Pasha, Muhammad Naveed, M. Waseem, M. Ahmad, Asad Raza
CRISPR–Cas genome editing technology developed from prokaryotes has transformed the molecular biology of plants past all assumptions. CRISPR–Cas, which is distinguished by its resilience, relatively high specificity, and easy implementation, enables specific genetic modification of crops, allowing for the creation of germplasms with favorable characters and the development of innovative, highly efficient agricultural systems. Moreover, many new biotechnologies in the framework of CRISPR–Cas platforms have bolstered basic research as well as synthetic biology toolkit of plants. In this article, initially, we provide a brief overview of CRISPR–Cas gene editing, emphasis on the modern, most specific gene-editing techniques, such as prime and base editing. Following that, the major role of CRISPR–Cas in plants in enhancing pesticide and disease resistance, quality, yield, breeding, and faster domestication are next discussed. In this review, we discuss the current advancements in plant biotechnology linked to CRISPR–Cas, such as CRISPR–Cas gene control, reagent conveyance, multiplexed gene editing, directed evolution, and mutagenesis. In the end, we talk about how this innovative technology may be used in the future.
从原核生物发展而来的CRISPR-Cas基因组编辑技术已经超越了所有假设,改变了植物的分子生物学。CRISPR-Cas的特点是其弹性、相对较高的特异性和易于实施,能够对作物进行特异性基因改造,从而创造出具有有利性状的种质,并开发出创新的、高效的农业系统。此外,在CRISPR-Cas平台框架下的许多新生物技术支持了植物的基础研究和合成生物学工具包。在本文中,首先,我们提供了CRISPR-Cas基因编辑的简要概述,重点介绍了现代,最具体的基因编辑技术,如引物和碱基编辑。接下来讨论了CRISPR-Cas在植物中增强抗农药和抗病性、品质、产量、育种和更快驯化等方面的主要作用。本文综述了与CRISPR-Cas相关的植物生物技术的最新进展,包括CRISPR-Cas基因控制、试剂传输、多重基因编辑、定向进化和诱变等。最后,我们讨论了这项创新技术在未来的应用。
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引用次数: 6
Indicator Plants of Perennial Streams: Ecological Approach for the Wildlife Management in Mining Areas 多年生河流指示植物:矿区野生动物管理的生态学途径
Pub Date : 2022-01-27 DOI: 10.9734/aprj/2022/v9i130194
S. Kumar N., Sinu Aradhana, Sugimani Marndi, Sanjeet Kumar
Mining activities create lot of ecological imbalance, including destruction of wildlife habitat. It also gives a negative impact on perennial stream. In near future, we have to restore them using ecological keys. Bio – indicator of perennial stream is very important for restoration and act like ecological keys. Keeping this in view, an attempt has been made to enumerate the plant species growing in and around perennial stream of Koira range, Bonai Forest Division, Odisha, India. Koira range comes under mining impacted areas. Results revealed that about 21 plant species are commonly available in 10 streams of Koira range. Enumerated species could be the indicators of perennial streams which would be fruitful for future restoration strategies.
采矿活动造成了许多生态失衡,包括破坏野生动物栖息地。同时对多年生河流也有负面影响。在不久的将来,我们必须使用生态钥匙来恢复它们。多年生河流的生物指标对恢复具有重要意义,是生态的关键。考虑到这一点,本文试图列举生长在印度奥里萨邦博奈森林科伊拉山脉多年生溪流及其周围的植物物种。科伊拉山脉位于受采矿影响的地区。结果表明,在科依拉山脉的10条河流中,常见的植物种类约有21种。列举的物种可以作为多年生河流的指标,为未来的恢复策略提供有益的参考。
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引用次数: 0
Taxonomic and Pharmacognostic Evaluation of Leaf of Mussaenda philippica. (Rubiaceae) 牡丹叶的分类与生药学评价。(茜草科)
Pub Date : 2022-01-27 DOI: 10.9734/aprj/2022/v9i130195
Romanus A. Umoh, I. Johnny, Anwanabasi E.Udoh, N. Andy, A. –, .. Essien, Inyang J. Udoh, Trust E. Ekpo, Gabriel U. Ashibeshi
Mussaenda philippica Linn. (Rubiaceae) a very important medicinal plant in the treatment of appendicitis and hepatitis as the locals use the species with the white flower. The objective of the study determines various taxonomic and pharmacognostic standards helpful to ensure the identity, purity, safety and efficacy of the medicinal plant, M. phillipica. The leaves were collected, identified, air dried and pulverized. Standard procedures were carried out to obtain microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, flourescence properties, soluble extractive values, moisture contents and ash values. The results of the microscopy study of the fresh leaf revealed hypostomatic distribution of stomata with paracytic stomata on the abaxial surface of the leaf only, stomatal number of 56.8, stomatal index of 25.06%, and epidermal number of 169.8, while the adaxial surface had an epidermal number of 241.9. The plant sample of the leaf also possessed unicellular trichomes. Micromeritic properties of the powdered leaf samples showed bulk volume of 35.33±0.33, tapped volume of 28.00±0.00, bulk density of 0.28±0.00, tapped density of 0.35±0.00, angle of repose of 35.4°, Carr’s Index of 20.90±0.74, Hausner’s ratio of 1.26±0.01. Chemomicroscopy study on the leaf powder revealed the presence of lignin, starch, cellulose, calcium oxalate crystals, oil, mucilage and protein. The moisture content was 11 %w/w. Results for the total ash, acid-insoluble ash and water-soluble ash values were 9 %w/w, 1%w/w and 5 %w/w respectively. Results for the ethanol-soluble, methanol-soluble and water-soluble extractive values were 18%w/w, 17 %w/w and 25 %w/w respectively. The above results could be used to establish pharmacopoeial standard of fresh and powdered drug of M. philippica.
菲利比卡林。(茜草科)是治疗阑尾炎和肝炎非常重要的药用植物,当地人使用该物种的白色花。研究的目的是确定各种分类和生药学标准,以帮助确保药用植物黄芩的身份、纯度、安全性和有效性。收集、鉴定、风干和粉碎叶子。采用标准程序获得新鲜和粉末状样品的显微特征、显微分析、化学显微观察、荧光特性、可溶性萃取物值、水分含量和灰分值。鲜叶显微镜观察结果显示,鲜叶仅在叶背面有气孔的下气孔分布,气孔数为56.8个,气孔指数为25.06%,表皮数为169.8个,而鲜叶正面表皮数为241.9个。叶子的植物样本也具有单细胞毛状体。叶粉样品的微分形性状为:容积为35.33±0.33,抽头体积为28.00±0.00,容重为0.28±0.00,抽头密度为0.35±0.00,休止角为35.4°,卡尔指数为20.90±0.74,豪斯纳比为1.26±0.01。对叶粉的化学显微镜研究发现木质素、淀粉、纤维素、草酸钙晶体、油、粘液和蛋白质的存在。含水率为11% w/w。结果表明,总灰分、酸不溶灰分和水溶性灰分分别为9%、1%和5% w/w。结果表明,乙醇溶、甲醇溶、水溶提取物的提取率分别为18%、17%、25%。上述结果可用于制定菲立比鲜药和粉药的药典标准。
{"title":"Taxonomic and Pharmacognostic Evaluation of Leaf of Mussaenda philippica. (Rubiaceae)","authors":"Romanus A. Umoh, I. Johnny, Anwanabasi E.Udoh, N. Andy, A. –, .. Essien, Inyang J. Udoh, Trust E. Ekpo, Gabriel U. Ashibeshi","doi":"10.9734/aprj/2022/v9i130195","DOIUrl":"https://doi.org/10.9734/aprj/2022/v9i130195","url":null,"abstract":"Mussaenda philippica Linn. (Rubiaceae) a very important medicinal plant in the treatment of appendicitis and hepatitis as the locals use the species with the white flower. The objective of the study determines various taxonomic and pharmacognostic standards helpful to ensure the identity, purity, safety and efficacy of the medicinal plant, M. phillipica. The leaves were collected, identified, air dried and pulverized. Standard procedures were carried out to obtain microscopic features of the fresh and powdered samples, micromeritic, chemomicroscopy, flourescence properties, soluble extractive values, moisture contents and ash values. The results of the microscopy study of the fresh leaf revealed hypostomatic distribution of stomata with paracytic stomata on the abaxial surface of the leaf only, stomatal number of 56.8, stomatal index of 25.06%, and epidermal number of 169.8, while the adaxial surface had an epidermal number of 241.9. The plant sample of the leaf also possessed unicellular trichomes. Micromeritic properties of the powdered leaf samples showed bulk volume of 35.33±0.33, tapped volume of 28.00±0.00, bulk density of 0.28±0.00, tapped density of 0.35±0.00, angle of repose of 35.4°, Carr’s Index of 20.90±0.74, Hausner’s ratio of 1.26±0.01. Chemomicroscopy study on the leaf powder revealed the presence of lignin, starch, cellulose, calcium oxalate crystals, oil, mucilage and protein. The moisture content was 11 %w/w. Results for the total ash, acid-insoluble ash and water-soluble ash values were 9 %w/w, 1%w/w and 5 %w/w respectively. Results for the ethanol-soluble, methanol-soluble and water-soluble extractive values were 18%w/w, 17 %w/w and 25 %w/w respectively. The above results could be used to establish pharmacopoeial standard of fresh and powdered drug of M. philippica.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85689847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In vitro Anti-inflammatory Activity of Endemic Artocarpus nobilis Thw Found in Sri Lanka 斯里兰卡特有种树树的体外抗炎活性研究
Pub Date : 2021-12-29 DOI: 10.9734/aprj/2021/v8i430192
S. Senadeera, K. Fernando, W. L. L. N. Wickramasekara, M. Fernando, C. Ranaweera, W. Rajapaksha, A. R. Silva
Aims: To investigate in vitro anti-inflammatory activity of aqueous, methanol, dichloromethane, and hexane extracts of Artocarpus nobilis Thw. leaves and stem bark using heat-induced protein denaturation test (egg albumin denaturation). Methodology: About 500 g of each matured, fully expanded leaves and stem bark of Artocarpus nobilis were collected, washed and air-dried. Leaves and stem bark parts were grounded to obtain a fine powder material. The extractions were obtained using the decoction extraction method. Anti-inflammatory activity was evaluated using the heat-induced egg albumin denaturation method. Diclofenac sodium was used as the positive control. Results: Results showed that Diclofenac sodium exhibited an IC50 value of 243.4 µg/mL and methanolic stem bark extract had an IC50 Value of 249.8 µg/mL) for heat-induced egg albumin protein denaturation test. R² and P values for aqueous, methanol, dichloromethane, and hexane extracts indicated that there was a strong, statistically significant correlation (P<0.01) between concentration and percentage inhibition for all extracts of A. nobilis Thw. However, methanol stem bark extract demonstrated the highest efficacy and potency with similar activity observed for the positive control Diclofenac sodium. Conclusion: Methanol stem bark extract of Artocarpus nobilis Thw. have marked in vitro anti-inflammatory activity. Further studies are necessary to determine the mechanism and the active constituents responsible for the anti-inflammatory activity of the plant parts of Artocarpus nobilis Thw.
目的:研究石竹水提液、甲醇提液、二氯甲烷提液和己烷提液的体外抗炎活性。叶和茎皮采用热诱导蛋白变性试验(蛋白蛋白变性)。方法:收集成熟、完全展开的鹿角树叶和茎皮各约500 g,清洗后风干。叶子和茎皮部分被磨碎以获得细粉末材料。采用煎提法提取。采用热诱导蛋清变性法评价其抗炎活性。双氯芬酸钠为阳性对照。结果:双氯芬酸钠对热致鸡蛋白蛋白变性试验的IC50值为243.4µg/mL,甲醇茎皮提取物对热致鸡蛋白蛋白变性试验的IC50值为249.8µg/mL。水提液、甲醇提液、二氯甲烷提液和己烷提液的R²和P值表明,各提液的浓度与百分抑制率之间存在显著的统计学相关性(P<0.01)。然而,甲醇茎皮提取物表现出最高的功效和效力,与阳性对照双氯芬酸钠的活性相似。结论:石竹茎皮甲醇提取物具有良好的药理作用。具有明显的体外抗炎活性。其抗炎作用机制及有效成分有待进一步研究。
{"title":"In vitro Anti-inflammatory Activity of Endemic Artocarpus nobilis Thw Found in Sri Lanka","authors":"S. Senadeera, K. Fernando, W. L. L. N. Wickramasekara, M. Fernando, C. Ranaweera, W. Rajapaksha, A. R. Silva","doi":"10.9734/aprj/2021/v8i430192","DOIUrl":"https://doi.org/10.9734/aprj/2021/v8i430192","url":null,"abstract":"Aims: To investigate in vitro anti-inflammatory activity of aqueous, methanol, dichloromethane, and hexane extracts of Artocarpus nobilis Thw. leaves and stem bark using heat-induced protein denaturation test (egg albumin denaturation). \u0000Methodology: About 500 g of each matured, fully expanded leaves and stem bark of Artocarpus nobilis were collected, washed and air-dried. Leaves and stem bark parts were grounded to obtain a fine powder material. The extractions were obtained using the decoction extraction method. Anti-inflammatory activity was evaluated using the heat-induced egg albumin denaturation method. Diclofenac sodium was used as the positive control. \u0000Results: Results showed that Diclofenac sodium exhibited an IC50 value of 243.4 µg/mL and methanolic stem bark extract had an IC50 Value of 249.8 µg/mL) for heat-induced egg albumin protein denaturation test. R² and P values for aqueous, methanol, dichloromethane, and hexane extracts indicated that there was a strong, statistically significant correlation (P<0.01) between concentration and percentage inhibition for all extracts of A. nobilis Thw. However, methanol stem bark extract demonstrated the highest efficacy and potency with similar activity observed for the positive control Diclofenac sodium. \u0000Conclusion: Methanol stem bark extract of Artocarpus nobilis Thw. have marked in vitro anti-inflammatory activity. Further studies are necessary to determine the mechanism and the active constituents responsible for the anti-inflammatory activity of the plant parts of Artocarpus nobilis Thw.","PeriodicalId":8546,"journal":{"name":"Asian Journal of Plant Science & Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79326096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
期刊
Asian Journal of Plant Science & Research
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