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Climate Services for Agriculture: Tools for Informing Decisions Relating to Climate Change and Climate Variability in the Wine Industry 农业气候服务:为葡萄酒行业气候变化和气候变率相关决策提供信息的工具
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-27 DOI: 10.1155/2023/5025359
L. Webb, C. Tozer, L. Bettio, R. Darbyshire, Bella Robinson, A. Fleming, Sigrid Tijs, R. Bodman, M. Prakash
Background and Aims. Australia’s changing climate is already impacting the agriculture sector and will continue to do so in the future. To help respond to these impacts, the Climate Services for Agriculture (CSA) platform presents readily accessible climate data, including future climate projections, relevant to specific agricultural commodities. This wine industry example aims to demonstrate the functionality and utility of the CSA for national use across a broad range of commodities. Methods and Results. The platform includes commodity-relevant climate indices designed in consultation with experts to ensure that they are as salient to producers as possible; the wine-grape specific indices include measures of growing season temperature, rainfall, extreme heat, and frost. Here, we describe the research behind the wine-grape specific indices and present sample outputs from the CSA platform for a site within a selected winegrowing region. We note the CSA platform has been developed through an extensive and continuing user engagement initiative, ensuring it meets the needs of the agriculture community as they grapple with how to make decisions based on longer term climate projections. Conclusions. Provision of past, seasonal outlook, and future climate information for Australia and for a range of important agricultural commodities can help improve on-farm planning and decision-making to respond to climate risks. The wine industry provides a leading example of how to use these data for decision-making, noting ongoing adjustments will be needed. Significance of the Study. The CSA platform brings together historical climate data, seasonal climate outlooks, and future climate projections to assist agricultural producers to better manage climate variability and climate change. It aims to nationalise this information for all major agricultural commodities in Australia. We use wine production as a demonstration case here.
背景和目的。澳大利亚不断变化的气候已经影响到农业部门,并将在未来继续这样做。为了帮助应对这些影响,农业气候服务(CSA)平台提供了易于获取的气候数据,包括与特定农产品相关的未来气候预测。这个葡萄酒行业的例子旨在展示CSA在全国范围内广泛使用的功能和效用。方法与结果。该平台包括与专家协商设计的与商品有关的气候指数,以确保这些指数对生产者尽可能突出;酿酒葡萄的具体指标包括生长期温度、降雨量、极端高温和霜冻。在这里,我们描述了葡萄酒-葡萄特定指数背后的研究,并展示了CSA平台在选定的葡萄酒产区内的一个站点的样本输出。我们注意到,CSA平台是通过广泛和持续的用户参与倡议发展起来的,确保它满足农业社区的需求,因为他们正在努力做出基于长期气候预测的决策。结论。提供澳大利亚和一系列重要农产品的过去、季节前景和未来气候信息,有助于改善农场规划和决策,以应对气候风险。葡萄酒行业为如何利用这些数据进行决策提供了一个领先的例子,并指出需要不断进行调整。研究的意义。CSA平台汇集了历史气候数据、季节气候展望和未来气候预测,以帮助农业生产者更好地管理气候变率和气候变化。它的目标是将澳大利亚所有主要农产品的信息国有化。我们在这里以葡萄酒生产为例。
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引用次数: 0
Modelling Smoke Flavour in Wine from Chemical Composition of Smoke-Exposed Grapes and Wine 从烟熏葡萄和葡萄酒的化学成分模拟葡萄酒中的烟味
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-21 DOI: 10.1155/2023/4964850
M. Parker, WenWen Maddy Jiang, E. Bilogrevic, D. Likos, John Gledhill, A. Coulter, G. Cowey, C. Simos, I. Francis, M. Herderich
Wine grapes exposed to smoke and wine made from grapes exposed to smoke can robustly be identified through their elevated concentrations of volatile phenols and phenolic glycosides serving as smoke markers, compared to concentrations typically found in non-smoke-exposed samples. Smoke-affected wines with high concentrations of volatile phenols and glycosides can have smoky flavours, but the relationship between concentrations of specific smoke markers in grapes and the intensity of smoky sensory attributes in the resulting wine has not been established. This study sought to determine whether volatile phenols and glycoside concentration in grapes and wine are suited to predict smoke flavour, to identify the key drivers of smoke flavour in both matrices. The study aimed to determine what concentrations of volatiles and glycosides in grapes impart an unacceptable smoke flavour in the resulting wine, to provide a guide for producers assessing suitability of smoke-exposed grapes for wine production. During vintage 2020, a total of 65 grape samples were collected from vineyards exposed to bushfire smoke, as well as unaffected vineyards. Chardonnay, Pinot Noir, and Shiraz grapes were harvested from vineyards in New South Wales, South Australia, and Victoria. Unoaked wines (50 kg scale) were produced under controlled conditions. The wines had a wide range of smoke flavour intensities rated by a trained sensory panel. Statistical models based on guaiacol, o-cresol, m-cresol, p-cresol, and some glycosides gave good predictions of smoke flavour intensity, with a slightly different optimal model for each cultivar. Subsequently, critical concentrations for quality defects were estimated to provide a guide for producers. A subset of smoke exposure markers in wine grapes affected by smoke from bushfires can be used to predict the degree of smoke flavour in wine. This information provides a first guide for assessing the risk of producing smoke tainted wine from smoke-exposed grapes.
与非烟雾暴露样品中的典型浓度相比,暴露在烟雾中的酿酒葡萄和由暴露在烟雾下的葡萄制成的葡萄酒可以通过其作为烟雾标记的挥发性酚类和酚苷类的浓度升高来进行有力的鉴定。含有高浓度挥发性酚类和糖苷的受烟雾影响的葡萄酒可能具有烟熏味,但葡萄中特定烟雾标记物的浓度与葡萄酒中烟熏感官特征的强度之间的关系尚未确定。本研究试图确定葡萄和葡萄酒中的挥发性酚类和糖苷浓度是否适合预测烟味,以确定两种基质中烟味的关键驱动因素。这项研究旨在确定葡萄中的挥发物和糖苷的浓度会在所得葡萄酒中产生不可接受的烟熏味,为生产商评估烟熏葡萄是否适合葡萄酒生产提供指导。在2020年份期间,共从暴露在山火烟雾中的葡萄园以及未受影响的葡萄园中采集了65个葡萄样本。霞多丽、黑皮诺和设拉子葡萄来自新南威尔士州、南澳大利亚州和维多利亚州的葡萄园。未酿造的葡萄酒(50 kg规模)在受控条件下生产。这些葡萄酒具有广泛的烟熏味强度,由经过培训的感官小组评定。基于愈创木酚、邻甲酚、间甲酚、对甲酚和一些糖苷的统计模型可以很好地预测烟味强度,每个品种的最佳模型略有不同。随后,对质量缺陷的临界浓度进行了估算,为生产商提供了指导。受山火烟雾影响的葡萄酒葡萄中的烟雾暴露标记子集可以用来预测葡萄酒中烟雾的味道程度。这些信息为评估暴露在烟雾中的葡萄生产烟雾污染葡萄酒的风险提供了第一个指南。
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引用次数: 1
Distribution of 3-Isobutyl-2-methoxypyrazine across Rachis Components of Vitis vinifera Shiraz and Cabernet Sauvignon 3-异丁基-2-甲氧基吡嗪在葡萄和赤霞珠Rachis组分中的分布
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-18 DOI: 10.1155/2023/2428791
Ross D. Sanders, P. Boss, D. Capone, Catherine M. Kidman, E. L. Nicholson, D. Jeffery
Rootstock can significantly alter the concentration of methoxypyrazines (MPs) in the bunch stem (rachis) of Vitis vinifera L. cv. Cabernet Sauvignon and Shiraz, which has implications for winemaking and wine style. The distribution of MPs across the rachis is an important consideration, but such information was not available. This study aimed to address this research question by comparing MP concentrations in different rachis components throughout grape maturation and in the absence of ambient light. Shiraz and Cabernet Sauvignon bunches were sampled throughout development, segmented into four components (peduncle, top rachis, bottom rachis, and pedicel), and 3-isobutyl-2-methoxypyrazine (IBMP) was quantified in each. For both cultivars, IBMP showed a negative correlation with grape maturity, with concentrations in pedicel at harvest being significantly higher than other rachis components. Additionally, light exclusion significantly increased IBMP concentrations in all rachis segments. The concentration of IBMP varied significantly between different rachis components. The greatest concentrations were measured in the pedicel, which also contributed the largest proportion among the components to total rachis by weight. Due to elevated IBMP concentrations in rachis and the difficulties in excluding matter other than grape from a fermentor, the presence of pedicel during fermentation could produce Shiraz and Cabernet Sauvignon wines with higher concentrations of MPs, thereby potentially increasing vegetal sensory characteristics.
砧木可以显著改变葡萄赤霞珠和设拉子束茎(轴)中甲氧基吡嗪(MP)的浓度,这对酿酒和葡萄酒风格有影响。MP在脊柱上的分布是一个重要的考虑因素,但没有这样的信息。本研究旨在通过比较葡萄成熟过程中和在没有环境光的情况下不同果穗轴成分中MP的浓度来解决这一研究问题。在整个发育过程中对设拉子和赤霞珠串进行取样,分为四种成分(蒂、顶轴、底轴和蒂),并对每种成分中的3-异丁基-2-甲氧基吡嗪(IBMP)进行定量。对于这两个品种,IBMP与葡萄成熟度呈负相关,收获时蒂中的浓度显著高于其他轴成分。此外,光排斥显著增加了所有轴段的IBMP浓度。IBMP的浓度在不同轴组分之间存在显著差异。在椎弓根中测得的浓度最大,按重量计算,椎弓根在各成分中占总轴的比例也最大。由于果穗轴中IBMP浓度升高,以及难以将葡萄以外的物质排除在发酵罐之外,发酵过程中出现果蒂可能会生产出MP浓度更高的设拉子和赤霞珠葡萄酒,从而潜在地提高植物的感官特征。
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引用次数: 1
Using Zeolites to Cold Stabilize White Wines 沸石冷稳定白葡萄酒的研究
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-07 DOI: 10.1155/2023/7259974
Tim Reilly, P. Mierczyński, A. Suwanto, Satriyo Krido Wahono, W. Maniukiewicz, K. Vasilev, K. Bindon, A. Mierczynska-Vasilev
Background and Aims. Tartrate stabilisation is a necessary step in commercial wine production. The traditional method to prevent crystallisation and precipitation of potassium bitartrate (KHT) after a wine is bottled is by adding seed KHT crystals to wine stored in a tank and holding temperatures below 0°C for a set period of time before bottling. This process requires time and energy and a filtration step to remove sediment. However, compared to other technical solutions such as reverse osmosis, electrodialysis, or ion exchange, it is still the most economical stabilisation option. This work aims to evaluate the ability of zeolites to cold stabilize white wines. Since zeolites can also remove proteins and thus heat-stabilize white wines, the new process can potentially combine heat and cold stability in a single treatment. Methods and Results. Effective tartrate stabilisation was achieved by mixing a natural zeolite sample with white wine for three hours. Although the quantum of required zeolite was larger than bentonite, zeolite did not exhibit shrink-swell behaviour, thus enabling greater wine recovery and capacity to be regenerated. Effective heat and cold stability could be achieved using a low-calcium zeolite as a processing aid in a single treatment. To avoid aluminium leaching and elevated aluminium concentrations in the treated wine, the zeolite was calcinated before being added to the wine. The calcination process also reduced calcium content in the wine after treatment with zeolite, thus eliminating the risk of calcium instability. Conclusions. The application of zeolite as a processing aid can potentially be effective in cold-stabilizing white wines and removing proteins responsible for haze formation. Significance of the study. Zeolites may constitute an alternative technology in white wine production facilitating heat and cold stabilisation in a single treatment.
背景和目的。稳定酒石酸盐是商业葡萄酒生产中的一个必要步骤。防止葡萄酒装瓶后酒石酸钾结晶和沉淀的传统方法是将种子KHT晶体添加到储存在罐中的葡萄酒中,并在装瓶前将温度保持在0°C以下一段时间。这个过程需要时间和精力,还需要一个过滤步骤来去除沉积物。然而,与反渗透、电渗析或离子交换等其他技术解决方案相比,它仍然是最经济的稳定选择。这项工作旨在评估沸石对白葡萄酒的冷稳定能力。由于沸石还可以去除蛋白质,从而使白葡萄酒热稳定,因此新工艺可能在一次处理中结合热稳定性和冷稳定性。方法和结果。通过将天然沸石样品与白葡萄酒混合三小时来实现有效的酒石酸盐稳定。尽管所需沸石的量比膨润土大,但沸石没有表现出收缩-膨胀行为,因此能够提高葡萄酒的回收率和再生能力。使用低钙沸石作为加工助剂可以在一次处理中实现有效的热稳定性和冷稳定性。为了避免处理过的葡萄酒中铝的浸出和铝浓度的升高,沸石在加入葡萄酒中之前进行了煅烧。煅烧过程还降低了用沸石处理后葡萄酒中的钙含量,从而消除了钙不稳定的风险。结论。沸石作为加工助剂的应用可能在冷稳定白葡萄酒和去除导致雾霾形成的蛋白质方面有效。研究的意义。沸石可能是白葡萄酒生产中的一种替代技术,有助于在单一处理中实现热稳定和冷稳定。
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引用次数: 0
Controlled Aeration Driven by Oxidation-Reduction Potential Affects Ester Profile in Wine Alcohol Fermentation with Different Starter Cultures 氧化还原电位驱动的控制曝气对不同发酵剂发酵葡萄酒中酯谱的影响
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-03-31 DOI: 10.1155/2023/5667458
S. Xue, L. Wang, S. Chen, X. H. Liu, K. Hu, J. Jiang, Y. Tao, G. Jin
Background and Aims. Aeration, an important operation in winemaking, cannot be controlled accurately based on dissolved oxygen for ester production in wine alcohol fermentation. The following study describes an aeration control method with oxidation-reduction potential (ORP) in alcohol fermentation and investigates its effect on ester production in fermentations with different starter cultures. Methods and Results. The proposed method is based on using ORP as a switch for aeration timing. Different aeration levels driven by ORP were performed in wine alcohol fermentation with different starter cultures including Saccharomyces cerevisiae (Sc), Pichia fermentans (Pf), Hanseniaspora uvarum (Hu), and their mixes (Pf/Sc and Hu/Sc). The accumulated aeration volume, residual sugar concentration, viable cell number, and ester concentration were analyzed. Results showed that aeration levels could be controlled effectively with an ORP value, and aeration with higher ORPs triggered faster sugar utilization in Sc and Hu/Sc fermentation. Pf and Hu survived one day less in their respective cofermentation with aeration when ORP was −100 mV or −50 mV compared to the natural ORP (−150 mV∼−105 mV in Pf/Sc and −141 mV∼−107 mV in Hu/Sc, respectively). Aeration driven by ORP changed ester profiles in cofermentations. With the aeration levels increasing, the proportion of medium-chain fatty acid ethyl esters in the concentration of total esters first increased and then decreased in Hu/Sc fermentation. When ORP was −100 mV in Pf/Sc fermentation, the proportion of higher alcohol acetates to total esters was highest (8.14%), while that of ethyl acetate to total esters was lowest (87.35%). Conclusions. Aeration driven by ORP improved wine ester profiles by increasing the proportion of medium-chain fatty acid ethyl esters or reducing that of ethyl acetate in cofermentations. Significance of the Study. The present study will allow wineries and researchers to optimize the aeration process in alcohol fermentation and develop a scientific aeration strategy to improve the wine ester profile and aroma quality.
背景和目的。曝气是酿酒中的一项重要操作,在葡萄酒酒精发酵生产酯时,不能根据溶解氧准确控制曝气。以下研究描述了酒精发酵中具有氧化还原电位(ORP)的曝气控制方法,并研究了其对不同发酵剂培养物发酵中酯生产的影响。方法和结果。所提出的方法是基于使用ORP作为曝气时间的开关。在酿酒酵母(Sc)、发酵毕赤酵母(Pf)、小曲汉塞菌(Hu)及其混合物(Pf/Sc和Hu/Sc)等不同发酵剂培养基的葡萄酒酒精发酵中,通过ORP驱动的不同通气水平进行了研究。分析了累积通气量、残留糖浓度、活细胞数和酯浓度。结果表明,ORP值可以有效地控制曝气水平,在Sc和Hu/Sc发酵中,ORP越高,糖利用率越高。当ORP为−100时,Pf和Hu在各自的通气共培养中存活时间减少了一天 mV或−50 mV与自然ORP(−150 mV~-105 Pf/Sc和−141中的mV mV~-107 分别为Hu/Sc中的mV)。ORP驱动的曝气改变了共发酵中的酯分布。在Hu/Sc发酵过程中,随着曝气量的增加,中链脂肪酸乙酯在总酯浓度中的比例先增大后减小。ORP为−100时 在Pf/Sc发酵过程中,高级醇乙酸酯占总酯的比例最高(8.14%),乙酸乙酯占总酯比例最低(87.35%)。ORP驱动的曝气通过增加中链脂肪酸乙酯的比例或减少共发酵中乙酸乙酯的比例来改善葡萄酒的酯型。研究的意义。本研究将使酿酒厂和研究人员能够优化酒精发酵中的曝气过程,并制定科学的曝气策略来改善葡萄酒的酯型和香气质量。
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引用次数: 0
The Microbial Terroir of the Nemea Zone Agiorgitiko cv.: A First Metataxonomic Approach Nemea地区微生物风土条件研究[j]。第一元分类学方法
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-03-17 DOI: 10.1155/2023/8791362
M. Kazou, Lena Pagiati, E. Dotsika, N. Proxenia, Y. Kotseridis, E. Tsakalidou
Vitis vinifera L. cv. Agiorgitiko is one of the most popular indigenous wine grape varieties in Greece, cultivated almost exclusively in the Nemea Protected Designation of Origin (PDO) zone. Here, the microbiota of soil, grapes, and wine, during controlled (CF) and spontaneous (SF) fermentations of Agiorgitko cv. from three vineyards in the PDO Nemea zone were explored, using both classical microbiological analysis and metataxonomics to get evidence about the microbial terroir of the PDO Nemea zone. The classical microbiological analysis revealed higher total mesophilic counts in soil, while in both grapes and wine samples, yeasts prevailed. Lactic acid bacteria and acetic acid bacteria counts were lower in grapes compared to wine and soil. Metataxonomic analysis revealed that, regarding yeasts/fungi, genera Fusarium, Sarea, and Alternaria dominated in soil; Aureobasidium, Cladosporium, and Penicillium in grapes; Saccharomyces in wine during CF; and Hanseniaspora and Saccharomyces in wine during SF. Regarding bacteria, genera Skermanella, Acidobacterium, and Ohtaekwangia dominated in soil, Sphingomonas, Micrococcus, and Rubrobacter in grapes, while Tatumella, Alcanivorax, and Komagateibacter in wine during both CF and SF. Finally, the factors that significantly influence the microbiota of soil, grapes, and wine samples were assessed, and potential microbial biomarkers were identified for the first time in a Greek grape variety.
葡萄(Vitis vinifera L.)agorgitiko是希腊最受欢迎的本土酿酒葡萄品种之一,几乎只在Nemea原产地指定保护(PDO)区域种植。在这里,agorgitko cv的控制(CF)和自然(SF)发酵过程中土壤、葡萄和葡萄酒的微生物群。对PDO Nemea地区的三个葡萄园进行了研究,使用经典微生物学分析和元类群学来获得PDO Nemea地区微生物风土的证据。经典的微生物分析显示,土壤中嗜温菌总数较高,而在葡萄和葡萄酒样品中,酵母占主导地位。与葡萄酒和土壤相比,葡萄中的乳酸菌和乙酸细菌数量较低。元分类分析表明,酵母菌/真菌中镰刀菌属、Sarea菌属和Alternaria菌属占主导地位;葡萄中的小孢子菌、枝孢子菌和青霉;CF过程中葡萄酒中的酵母菌;SF期间葡萄酒中的汉森菌和酵母菌。细菌方面,CF和SF期间土壤中以Skermanella、Acidobacterium和Ohtaekwangia属为主,葡萄中以Sphingomonas、Micrococcus和Rubrobacter属为主,葡萄酒中以Tatumella、Alcanivorax和Komagateibacter属为主。最后,对影响土壤、葡萄和葡萄酒样品微生物群的因素进行了评估,并首次在希腊葡萄品种中鉴定了潜在的微生物生物标志物。
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引用次数: 1
Assessment of the Crop Forcing Technique and Irrigation Strategy on the Ripening of Tempranillo Grapes in a Semiarid Climate 半干旱气候条件下甜葡萄催熟技术及灌溉策略的评价
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-03-07 DOI: 10.1155/2023/6278665
Nieves Lavado, D. Uriarte, L. Mancha, D. Moreno, M. Valdés, M. Henar Prieto
Background and Aims. High temperatures during grape ripening have a negative effect on the winemaking characteristics of musts. The crop forcing technique delays ripening to a period when temperatures are lower. The objective of this study is to provide information to winemakers and grape growers on how the delay in ripening caused by crop forcing can affect berry performance. Methods and Results. This study of 3 growing seasons (2017–2019) analyzes the effect of this technique in a vineyard of the Vitis vinifera L. cv. Tempranillo in Extremadura, together with two irrigation strategies. The grapevines were forced 4 and 22 days after anthesis (F1 and F2, respectively), compared to a treatment without crop forcing techniques (NF). Each treatment was subjected to two irrigation strategies: to cover the water needs of the plants (C) and deficit irrigation during preveraison (RI). Crop forcing delayed the harvest between 32 and 56 days on average in relation to NF. Crop forcing and irrigation strategy modified berry composition at harvest: C-F1 and C-F2 had higher total polyphenol and anthocyanin concentrations, total acidity, malic acid content, and lower pH relative to C-NF; RI-NF increased total anthocyanin concentration and pH and decreased titratable acidity value. Conclusions. Crop forcing is able to delay grape ripening to lower temperature periods. This is a promising technique for restoring the coupling between phenolic and technological ripeness. The combination of both crop forcing and deficit irrigation strategy maintains the berry quality while improving water use efficiency. Significance of the Study. The present work shows how the “crop-forcing” technique is effective in modifying the relationship between different parameters that determine the characteristics of berries throughout ripening as a raw material for winemaking, compared to vines with traditional winter pruning, under different irrigation strategies.To the memory of Jordi Marsal
背景和目的。葡萄成熟过程中的高温对葡萄酒的酿造特性有负面影响。催熟技术将催熟推迟到温度较低的时期。本研究的目的是为酿酒师和葡萄种植者提供信息,了解作物催熟造成的延迟如何影响浆果的性能。方法与结果。本研究对葡萄(Vitis vinifera L. cv.)的三个生长季节(2017-2019)进行了研究,分析了该技术在葡萄园中的效果。埃斯特雷马杜拉的丹魄,以及两种灌溉策略。与不采用作物催收技术(NF)的处理相比,在葡萄开花后4天和22天(分别为F1和F2)进行催收。每个处理都有两种灌溉策略:满足植物的水需求(C)和孕前亏缺灌溉(RI)。作物强迫使收获延迟了32 - 56天。作物胁迫和灌溉策略改变了收获期浆果成分:相对于C-NF, C-F1和C-F2具有较高的总多酚和花青素浓度、总酸度、苹果酸含量和较低的pH;RI-NF增加了总花青素浓度和pH值,降低了可滴定酸度值。结论。作物强迫能够延迟葡萄成熟到较低的温度时期。这是一种很有前途的恢复酚醛与工艺成熟度之间耦合的技术。作物强迫与亏缺灌溉策略相结合,既能保持果实品质,又能提高水分利用效率。研究的意义。目前的工作表明,与传统的冬季修剪葡萄藤相比,在不同的灌溉策略下,“作物强迫”技术如何有效地改变不同参数之间的关系,这些参数决定了作为酿酒原料的浆果在整个成熟过程中的特征。纪念乔迪·马绍尔
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引用次数: 2
Biomimetic Strategies for Peripheral Nerve Injury Repair: An Exploration of Microarchitecture and Cellularization. 外周神经损伤修复的仿生策略:微结构和细胞化的探索。
3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-03-01 Epub Date: 2022-10-03 DOI: 10.1007/s44174-022-00039-8
Jeremy M Perrelle, Andrew J Boreland, Jasmine M Gamboa, Prarthana Gowda, N Sanjeeva Murthy

Injuries to the nervous system present formidable challenges to scientists, clinicians, and patients. While regeneration within the central nervous system is minimal, peripheral nerves can regenerate, albeit with limitations. The regenerative mechanisms of the peripheral nervous system thus provide fertile ground for clinical and scientific advancement, and opportunities to learn fundamental lessons regarding nerve behavior in the context of regeneration, particularly the relationship of axons to their support cells and the extracellular matrix environment. However, few current interventions adequately address peripheral nerve injuries. This article aims to elucidate areas in which progress might be made toward developing better interventions, particularly using synthetic nerve grafts. The article first provides a thorough review of peripheral nerve anatomy, physiology, and the regenerative mechanisms that occur in response to injury. This is followed by a discussion of currently available interventions for peripheral nerve injuries. Promising biomaterial fabrication techniques which aim to recapitulate nerve architecture, along with approaches to enhancing these biomaterial scaffolds with growth factors and cellular components, are then described. The final section elucidates specific considerations when developing nerve grafts, including utilizing induced pluripotent stem cells, Schwann cells, nerve growth factors, and multilayered structures that mimic the architectures of the natural nerve.

神经系统的损伤给科学家、临床医生和病人带来了严峻的挑战。虽然中枢神经系统的再生能力微乎其微,但周围神经却可以再生,尽管有一定的局限性。因此,周围神经系统的再生机制为临床和科学进步提供了肥沃的土壤,也为我们提供了在再生过程中学习神经行为基本知识的机会,特别是轴突与其支持细胞和细胞外基质环境之间的关系。然而,目前很少有干预措施能充分解决周围神经损伤问题。本文旨在阐明可在哪些领域取得进展,以开发更好的干预措施,特别是使用合成神经移植物。文章首先全面回顾了周围神经解剖学、生理学以及损伤后的再生机制。随后讨论了目前可用于治疗周围神经损伤的干预措施。然后介绍了旨在再现神经结构的前景看好的生物材料制造技术,以及用生长因子和细胞成分增强这些生物材料支架的方法。最后一部分阐明了开发神经移植物时的具体注意事项,包括利用诱导多能干细胞、许旺细胞、神经生长因子和模仿天然神经结构的多层结构。
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引用次数: 0
In-the-Field Authentication of Grapevine (Vitis vinifera L.) cv: Albariño Using Chlorotype Discrimination and a Single SNP Interrogation by LAMP 葡萄(Vitis vinifera L.) cv: Albariño的叶绿体鉴别和LAMP单SNP鉴定
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-02-28 DOI: 10.1155/2023/2117139
J. Quinteiro, Lara Quinteiro, Ángela Díaz-Fernández, M. Rey-Méndez, J. Ibáñez, E. Díaz-Losada
Authentication of grapevine cultivars, Vitis vinifera L., is difficult, especially when analytical specimens lack diagnostic ampelographic characters, which prevents the verification of traceability systems aimed at guaranteeing varietal integrity. This issue is problematic when viticultural strategies and regulations associated with high-value wine-producing geographical areas rely on extensive control and monitoring of valuable cultivars. Varietal identification based on grapevine molecular markers is a standardized methodology that requires a specialised laboratory for its application. In contrast, the use of loop-mediated isothermal amplification (LAMP) allows DNA markers to be characterized quickly and easily, without the need for skilled personnel, allowing implementation in-situ or in-the-field. Simultaneous identification of the chlorotype and the interrogation of a single SNP using a portable device have allowed the first discrimination in-the-field of grafted grapevines, without appreciable ampelographic characters, as belonging to the valuable “Albariño” cultivar. This methodology constitutes a valuable tool for cultivar discrimination and can be efficiently implemented in the traceability of valuable grapevine genetic resources.
葡萄品种Vitis vinifera L.的鉴定很困难,尤其是当分析样品缺乏诊断性的图谱特征时,这阻碍了旨在保证品种完整性的可追溯系统的验证。当与高价值葡萄酒生产地理区域相关的葡萄栽培策略和法规依赖于对有价值品种的广泛控制和监测时,这个问题就成了问题。基于葡萄分子标记的品种鉴定是一种标准化的方法,需要专门的实验室进行应用。相反,环介导的等温扩增(LAMP)的使用允许在不需要熟练人员的情况下快速而容易地表征DNA标记,从而允许原位或现场实施。使用便携式设备同时识别氯型和询问单个SNP,使嫁接葡萄藤领域首次出现了属于有价值的“Albariño”品种的歧视,没有明显的葡萄图谱特征。这种方法是一种有价值的品种鉴别工具,可以有效地用于追踪有价值的葡萄遗传资源。
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引用次数: 1
Effects of Yeast Strain and Juice Nitrogen Status on Glutathione Utilisation during Fermentation of Model Media 酵母菌株和汁氮状况对模型培养基发酵过程中谷胱甘肽利用率的影响
IF 2.8 3区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-02-09 DOI: 10.1155/2023/8041096
M. Bekker, Kathleen M. Cuijvers, Allie C. Kulcsar, Ross D. Sanders, D. Capone, D. Jeffery, S. Schmidt
Background and Aims. An OIV resolution provides guidelines on using glutathione as a prefermentation additive when the amount of yeast assimilable nitrogen (YAN) of a juice or must is adequate, to avoid the metabolism of glutathione by the yeast. The effect of YAN concentration on glutathione metabolism by yeast had not been determined. This study explored whether nitrogen management could be used to control glutathione consumption during fermentation. Methods and Results. An HPLC-UV method was developed to quantify reduced L-glutathione (GSH) and oxidised glutathione (GSSG) and used to monitor yeast GSH metabolism during alcoholic fermentation with two yeast strains (AWRI 1688 and AWRI 2861). The addition of GSH had no impact on the fermentation rate of the chemically defined medium, even in a limited YAN environment; however, a decrease in glutathione concentration occurred regardless of YAN concentration. The effect of GSH on volatile sulfur compound formation was yeast strain-dependent. Conclusions. Increasing the YAN status of a chemically defined medium led to a decrease in GSH consumption during fermentation, but the loss of GSH could not be prevented entirely, even with a low initial GSH concentration and high initial YAN. Significance of the Study. In the presence of higher concentrations of GSH during fermentation, there is a risk of forming undesirable fermentative sulfur compounds that are not mitigated through nitrogen supplementation. Thus, it seems unlikely that an argument could be made for the inclusion of GSH in relevant food standards codes as a wine additive especially if a lack of GSH metabolism was a criterion.
背景和目的。OIV决议提供了在果汁或果汁中酵母可同化氮(YAN)的量足够时使用谷胱甘肽作为预发酵添加剂的指南,以避免酵母代谢谷胱甘肽。YAN浓度对酵母谷胱甘肽代谢的影响尚未确定。本研究探讨了氮管理是否可以用于控制发酵过程中谷胱甘肽的消耗。方法和结果。开发了一种HPLC-UV方法来定量还原型谷胱甘肽(GSH)和氧化型谷胱甘肽(GSSG),并用于监测两种酵母菌株(AWRI1688和AWRI2861)在酒精发酵过程中的酵母GSH代谢。GSH的添加对化学限定培养基的发酵速率没有影响,即使在有限的YAN环境中也是如此;然而,无论YAN浓度如何,谷胱甘肽浓度都会下降。GSH对挥发性硫化合物形成的影响是酵母菌株依赖性的。结论。化学定义培养基的YAN状态的增加导致发酵过程中GSH消耗的减少,但即使在低初始GSH浓度和高初始YAN的情况下,也不能完全防止GSH的损失。研究的意义。在发酵过程中存在更高浓度的GSH的情况下,存在形成不希望的发酵硫化合物的风险,这些化合物不能通过补充氮来减轻。因此,似乎不太可能将谷胱甘肽作为葡萄酒添加剂纳入相关食品标准法规,特别是如果缺乏谷胱甘肽代谢是一个标准的话。
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引用次数: 0
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Australian Journal of Grape and Wine Research
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