Australian Journal of Grape and Wine Research最新文献

Background and Aims. Grape phylloxera in Australia comprises diverse genetic strains that feed on roots and leaves of Vitis spp. The G38 phylloxera strain was detected on roots of Vitis spp., for the first time in North East Victoria in 2015. Prior to 2015, G38 phylloxera was only known to feed on leaves. The aim of this study was to evaluate the survival and development of G38 phylloxera on roots of diverse Vitis spp. under field, controlled laboratory, and greenhouse conditions. Methods and Results. In the field, emergence traps quantified first instars and alates emerging from roots of diverse rootstocks and Vitis vinifera L. High numbers of phylloxera were collected in traps placed at vines of rootstocks 101-14, 3309 Courderc and Schwarzmann. Nodosity were also observed on roots of 101-14, 3309 Courderc and Schwarzmann in the field and in-pot vines experiments. The better performance of G38 phylloxera on these three rootstocks compared to V. vinifera in the field and in potted vines parallelled the excised roots experiments. Conclusions. The relatively high performance of G38 phylloxera on the 101-14, 3309 Courderc and Schwarzmann rootstocks suggest a susceptible response and could be associated with rootstock parentage. Further investigation is warranted to determine implications for rootstocks development. Significance of the Study. These findings are fundamental for decision-making in phylloxera risk assessment and rootstock selection. The study reaffirms the need for triphasic (in vitro, in planta, and in-field) rootstock screening protocols for phylloxera.

Background and Aims. Shiraz disease (SD) is a viral disease associated with Grapevine virus A that causes significant yield loss in economically important grape cultivars in Australia such as Shiraz and Merlot. Current diagnostic methods are time-consuming and costly. This study evaluates an alternative methodology using visible remote sensing imagery to detect SD in Shiraz grapevines. Methods and Results. High-resolution visible remote sensing images were captured of Shiraz grapevines in two South Australian viticultural regions over two seasons. The projected leaf area (PLA) of individual grapevines was estimated from the images. Virus-infected vines had significantly lower PLA than healthy vines in the early season but fewer difference after veraison. The lower PLA was only observed in grapevines coinfected with grapevine leafroll-associated viruses (GLRaVs) and Grapevine virus A (GVA). Shiraz vines infected with either GLRaVs or GVA had similar PLA to healthy vines. Conclusions. High-resolution RGB remote sensing technology has the potential to rapidly estimate SD infection in Shiraz grapevines. Our observations of shoot devigouration only in coinfected vines calls into question the etiology of SD. Further validation of the PLA technique incorporating different regions, seasons, cultivars, and combinations of viruses is needed for improving the robustness of the method. Significance of the Study. This preliminary study presents a new rapid and low-cost surveillance method to estimate SD infections in Shiraz vineyards, which could significantly lower the cost for growers who conduct on-ground SD visual assessments or lab-based tissue testing at the vineyard scale.

Background and Aims. Australia’s changing climate is already impacting the agriculture sector and will continue to do so in the future. To help respond to these impacts, the Climate Services for Agriculture (CSA) platform presents readily accessible climate data, including future climate projections, relevant to specific agricultural commodities. This wine industry example aims to demonstrate the functionality and utility of the CSA for national use across a broad range of commodities. Methods and Results. The platform includes commodity-relevant climate indices designed in consultation with experts to ensure that they are as salient to producers as possible; the wine-grape specific indices include measures of growing season temperature, rainfall, extreme heat, and frost. Here, we describe the research behind the wine-grape specific indices and present sample outputs from the CSA platform for a site within a selected winegrowing region. We note the CSA platform has been developed through an extensive and continuing user engagement initiative, ensuring it meets the needs of the agriculture community as they grapple with how to make decisions based on longer term climate projections. Conclusions. Provision of past, seasonal outlook, and future climate information for Australia and for a range of important agricultural commodities can help improve on-farm planning and decision-making to respond to climate risks. The wine industry provides a leading example of how to use these data for decision-making, noting ongoing adjustments will be needed. Significance of the Study. The CSA platform brings together historical climate data, seasonal climate outlooks, and future climate projections to assist agricultural producers to better manage climate variability and climate change. It aims to nationalise this information for all major agricultural commodities in Australia. We use wine production as a demonstration case here.

Wine grapes exposed to smoke and wine made from grapes exposed to smoke can robustly be identified through their elevated concentrations of volatile phenols and phenolic glycosides serving as smoke markers, compared to concentrations typically found in non-smoke-exposed samples. Smoke-affected wines with high concentrations of volatile phenols and glycosides can have smoky flavours, but the relationship between concentrations of specific smoke markers in grapes and the intensity of smoky sensory attributes in the resulting wine has not been established. This study sought to determine whether volatile phenols and glycoside concentration in grapes and wine are suited to predict smoke flavour, to identify the key drivers of smoke flavour in both matrices. The study aimed to determine what concentrations of volatiles and glycosides in grapes impart an unacceptable smoke flavour in the resulting wine, to provide a guide for producers assessing suitability of smoke-exposed grapes for wine production. During vintage 2020, a total of 65 grape samples were collected from vineyards exposed to bushfire smoke, as well as unaffected vineyards. Chardonnay, Pinot Noir, and Shiraz grapes were harvested from vineyards in New South Wales, South Australia, and Victoria. Unoaked wines (50 kg scale) were produced under controlled conditions. The wines had a wide range of smoke flavour intensities rated by a trained sensory panel. Statistical models based on guaiacol, o-cresol, m-cresol, p-cresol, and some glycosides gave good predictions of smoke flavour intensity, with a slightly different optimal model for each cultivar. Subsequently, critical concentrations for quality defects were estimated to provide a guide for producers. A subset of smoke exposure markers in wine grapes affected by smoke from bushfires can be used to predict the degree of smoke flavour in wine. This information provides a first guide for assessing the risk of producing smoke tainted wine from smoke-exposed grapes.

Rootstock can significantly alter the concentration of methoxypyrazines (MPs) in the bunch stem (rachis) of Vitis vinifera L. cv. Cabernet Sauvignon and Shiraz, which has implications for winemaking and wine style. The distribution of MPs across the rachis is an important consideration, but such information was not available. This study aimed to address this research question by comparing MP concentrations in different rachis components throughout grape maturation and in the absence of ambient light. Shiraz and Cabernet Sauvignon bunches were sampled throughout development, segmented into four components (peduncle, top rachis, bottom rachis, and pedicel), and 3-isobutyl-2-methoxypyrazine (IBMP) was quantified in each. For both cultivars, IBMP showed a negative correlation with grape maturity, with concentrations in pedicel at harvest being significantly higher than other rachis components. Additionally, light exclusion significantly increased IBMP concentrations in all rachis segments. The concentration of IBMP varied significantly between different rachis components. The greatest concentrations were measured in the pedicel, which also contributed the largest proportion among the components to total rachis by weight. Due to elevated IBMP concentrations in rachis and the difficulties in excluding matter other than grape from a fermentor, the presence of pedicel during fermentation could produce Shiraz and Cabernet Sauvignon wines with higher concentrations of MPs, thereby potentially increasing vegetal sensory characteristics.

Background and Aims. Tartrate stabilisation is a necessary step in commercial wine production. The traditional method to prevent crystallisation and precipitation of potassium bitartrate (KHT) after a wine is bottled is by adding seed KHT crystals to wine stored in a tank and holding temperatures below 0°C for a set period of time before bottling. This process requires time and energy and a filtration step to remove sediment. However, compared to other technical solutions such as reverse osmosis, electrodialysis, or ion exchange, it is still the most economical stabilisation option. This work aims to evaluate the ability of zeolites to cold stabilize white wines. Since zeolites can also remove proteins and thus heat-stabilize white wines, the new process can potentially combine heat and cold stability in a single treatment. Methods and Results. Effective tartrate stabilisation was achieved by mixing a natural zeolite sample with white wine for three hours. Although the quantum of required zeolite was larger than bentonite, zeolite did not exhibit shrink-swell behaviour, thus enabling greater wine recovery and capacity to be regenerated. Effective heat and cold stability could be achieved using a low-calcium zeolite as a processing aid in a single treatment. To avoid aluminium leaching and elevated aluminium concentrations in the treated wine, the zeolite was calcinated before being added to the wine. The calcination process also reduced calcium content in the wine after treatment with zeolite, thus eliminating the risk of calcium instability. Conclusions. The application of zeolite as a processing aid can potentially be effective in cold-stabilizing white wines and removing proteins responsible for haze formation. Significance of the study. Zeolites may constitute an alternative technology in white wine production facilitating heat and cold stabilisation in a single treatment.

Background and Aims. Aeration, an important operation in winemaking, cannot be controlled accurately based on dissolved oxygen for ester production in wine alcohol fermentation. The following study describes an aeration control method with oxidation-reduction potential (ORP) in alcohol fermentation and investigates its effect on ester production in fermentations with different starter cultures. Methods and Results. The proposed method is based on using ORP as a switch for aeration timing. Different aeration levels driven by ORP were performed in wine alcohol fermentation with different starter cultures including Saccharomyces cerevisiae (Sc), Pichia fermentans (Pf), Hanseniaspora uvarum (Hu), and their mixes (Pf/Sc and Hu/Sc). The accumulated aeration volume, residual sugar concentration, viable cell number, and ester concentration were analyzed. Results showed that aeration levels could be controlled effectively with an ORP value, and aeration with higher ORPs triggered faster sugar utilization in Sc and Hu/Sc fermentation. Pf and Hu survived one day less in their respective cofermentation with aeration when ORP was −100 mV or −50 mV compared to the natural ORP (−150 mV∼−105 mV in Pf/Sc and −141 mV∼−107 mV in Hu/Sc, respectively). Aeration driven by ORP changed ester profiles in cofermentations. With the aeration levels increasing, the proportion of medium-chain fatty acid ethyl esters in the concentration of total esters first increased and then decreased in Hu/Sc fermentation. When ORP was −100 mV in Pf/Sc fermentation, the proportion of higher alcohol acetates to total esters was highest (8.14%), while that of ethyl acetate to total esters was lowest (87.35%). Conclusions. Aeration driven by ORP improved wine ester profiles by increasing the proportion of medium-chain fatty acid ethyl esters or reducing that of ethyl acetate in cofermentations. Significance of the Study. The present study will allow wineries and researchers to optimize the aeration process in alcohol fermentation and develop a scientific aeration strategy to improve the wine ester profile and aroma quality.

Vitis vinifera L. cv. Agiorgitiko is one of the most popular indigenous wine grape varieties in Greece, cultivated almost exclusively in the Nemea Protected Designation of Origin (PDO) zone. Here, the microbiota of soil, grapes, and wine, during controlled (CF) and spontaneous (SF) fermentations of Agiorgitko cv. from three vineyards in the PDO Nemea zone were explored, using both classical microbiological analysis and metataxonomics to get evidence about the microbial terroir of the PDO Nemea zone. The classical microbiological analysis revealed higher total mesophilic counts in soil, while in both grapes and wine samples, yeasts prevailed. Lactic acid bacteria and acetic acid bacteria counts were lower in grapes compared to wine and soil. Metataxonomic analysis revealed that, regarding yeasts/fungi, genera Fusarium, Sarea, and Alternaria dominated in soil; Aureobasidium, Cladosporium, and Penicillium in grapes; Saccharomyces in wine during CF; and Hanseniaspora and Saccharomyces in wine during SF. Regarding bacteria, genera Skermanella, Acidobacterium, and Ohtaekwangia dominated in soil, Sphingomonas, Micrococcus, and Rubrobacter in grapes, while Tatumella, Alcanivorax, and Komagateibacter in wine during both CF and SF. Finally, the factors that significantly influence the microbiota of soil, grapes, and wine samples were assessed, and potential microbial biomarkers were identified for the first time in a Greek grape variety.

Background and Aims. High temperatures during grape ripening have a negative effect on the winemaking characteristics of musts. The crop forcing technique delays ripening to a period when temperatures are lower. The objective of this study is to provide information to winemakers and grape growers on how the delay in ripening caused by crop forcing can affect berry performance. Methods and Results. This study of 3 growing seasons (2017–2019) analyzes the effect of this technique in a vineyard of the Vitis vinifera L. cv. Tempranillo in Extremadura, together with two irrigation strategies. The grapevines were forced 4 and 22 days after anthesis (F1 and F2, respectively), compared to a treatment without crop forcing techniques (NF). Each treatment was subjected to two irrigation strategies: to cover the water needs of the plants (C) and deficit irrigation during preveraison (RI). Crop forcing delayed the harvest between 32 and 56 days on average in relation to NF. Crop forcing and irrigation strategy modified berry composition at harvest: C-F1 and C-F2 had higher total polyphenol and anthocyanin concentrations, total acidity, malic acid content, and lower pH relative to C-NF; RI-NF increased total anthocyanin concentration and pH and decreased titratable acidity value. Conclusions. Crop forcing is able to delay grape ripening to lower temperature periods. This is a promising technique for restoring the coupling between phenolic and technological ripeness. The combination of both crop forcing and deficit irrigation strategy maintains the berry quality while improving water use efficiency. Significance of the Study. The present work shows how the “crop-forcing” technique is effective in modifying the relationship between different parameters that determine the characteristics of berries throughout ripening as a raw material for winemaking, compared to vines with traditional winter pruning, under different irrigation strategies.

To the memory of Jordi Marsal