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The occurrence and severity of grass toxicoses in Australian alpaca (Vicugna pacos) herds 澳大利亚羊驼(Vicugna pacos)群草中毒的发生和严重程度
Pub Date : 2008-08-04 DOI: 10.1071/EA06325
Natália G. Sampaio, M. Gishen, K. Reed, Melessa Brown, D. Gregory, K. Munyard
A survey of 108 alpaca producers registered with the Australian Alpaca Association examined the occurrence and severity of ‘staggers’ (a colloquial term embracing various diseases of the nervous system in animals, characterised by neck tremors and head nodding in the milder alpaca cases and a lack of coordination in moving, a staggering gait and frequent falling in severe cases) in Australian alpaca and the presence of four pasture grasses, during three production seasons in 2004–2006. There have been few studies on the susceptibility of alpaca to staggers and its effect on productivity and animal welfare. The survey found that 23% of alpaca producers had observed staggering animals, with Victoria and South Australia being the most severely affected states. Clinical signs of staggers were most frequent in January–March, with a mean duration of 3 months. Some animals showed clinical signs lasting up to 12 months. A strong correlation was found between the presence of perennial ryegrass (P < 0.001) and phalaris (P < 0.003) and the occurrence of staggers. Based on grass presence and the timing when staggers was observed, it was concluded that perennial ryegrass toxicosis was the main cause of staggers in alpaca. About 12% and 9% of alpaca grazing pasture containing perennial ryegrass exhibited staggers in the two full seasons for which data were collected. Herds with staggering animals were correlated with those exhibiting possible subclinical effects – heat sensitivity and ill-thrift (P < 0.01) and reduced fertility (P < 0.05) – of perennial ryegrass endophyte toxins. Results indicate that weanlings and cria are more likely to stagger than adults. Thirteen of the 15 mixed farms with staggering alpaca did not observe staggers in other livestock, suggesting that alpaca may be more sensitive to the causal toxins than sheep and cattle.
在2004-2006年的三个生产季节,对在澳大利亚羊驼协会注册的108个羊驼生产者进行了调查,调查了澳大利亚羊驼的“蹒跚症”(一种口语化的术语,包括动物的各种神经系统疾病,其特点是轻度羊驼的颈部震颤和点头,严重的羊驼的移动不协调、步态蹒跚和经常摔倒)的发生和严重程度,以及四种牧草的存在。羊驼对错落的易感性及其对生产力和动物福利的影响的研究很少。调查发现,23%的羊驼生产商发现了令人震惊的动物,维多利亚州和南澳大利亚州是受影响最严重的州。临床症状以1 - 3月为多见,平均病程3个月。一些动物表现出持续长达12个月的临床症状。多年生黑麦草(P < 0.001)和phalaris (P < 0.003)的存在与错落草的发生有较强的相关性。根据草的存在及发生错动的时间,认为多年生黑麦草中毒是羊驼错动的主要原因。有多年生黑麦草的羊驼牧场在两个完整的季节中分别有12%和9%的羊驼牧场出现错落现象。多年生黑麦草内生毒素对畜群的亚临床效应(热敏性、不节俭性)和生育力降低(P < 0.05)与畜群间存在显著相关性。研究结果表明,断奶婴儿和婴儿比成年人更容易摇晃。在15个混合饲养羊驼的农场中,有13个农场没有观察到其他牲畜的蹒跚,这表明羊驼可能比羊和牛对致病毒素更敏感。
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引用次数: 7
Dietary cation–anion differences in some pasture species, changes during the season and effects of soil acidity and lime amendment 牧草品种饲粮正负离子差异、季节变化及土壤酸度和石灰改良剂的影响
Pub Date : 2008-08-04 DOI: 10.1071/EA08121
S. Pelletier, R. Simpson, R. Culvenor, G. Bélanger, G. Tremblay, G. Allard, J. Braschkat, P. Randall
The difference between cation and anion concentrations is an important property when assessing feed for dry dairy cows in order to avoid hypocalcaemia following calving. Dietary cation–anion difference (DCAD) is used to assess suitability of feed and predict the risk of milk fever; a value of –5 cmol(+)/kg dry matter (DM) or less is desirable. This work has examined the DCAD of 16 field-grown species found in pasture in southern Australia. The DCAD [cmol(+)/kg DM] at the flowering stage varied from 7 to 32 for grasses, 21 to 72 for legumes and 72 to 99 for dicot weeds. The average DCAD for legumes was 50 cmol(+)/kg DM, over 2-fold higher than the 20 cmol(+)/kg DM average for grasses. There was a substantial decline in DCAD of herbage as the season progressed. In a glasshouse experiment with five grass species in an acid soil, lime application increased yield and tended to lower the DCAD. Lime decreased uptake per unit root length of potassium and chlorine and increased uptake of calcium by phalaris and timothy. While DCAD is an important attribute of herbage for assessing its suitability for prepartum diets of dairy cows, the present data indicate that it would be prudent to also consider concentrations of calcium and other mineral nutrients in herbage, particularly when examining less familiar plant species or the effects of different cultural practices on the composition of herbage for such diets.
为了避免产犊后低钙血症,在评估干奶牛饲料时,正离子和阴离子浓度的差异是一个重要的特性。采用日粮阴阳离子差(DCAD)评价饲料适宜性,预测乳热风险;理想值为- 5cmol (+)/kg干物质(DM)或更低。这项工作检查了在澳大利亚南部牧场发现的16种田间种植物种的DCAD。禾本科植物开花期DCAD [cmol(+)/kg DM]为7 ~ 32,豆科植物为21 ~ 72,双科杂草为72 ~ 99。豆科植物的平均DCAD为50 cmol(+)/kg DM,比禾本科植物的平均20 cmol(+)/kg DM高出2倍以上。随着季节的进展,牧草的DCAD有明显的下降。在酸性土壤中对5种禾草进行的温室试验中,施石灰提高了产量,并有降低DCAD的趋势。石灰降低了phalaris和timothym单位根长对钾和氯的吸收,增加了钙的吸收。虽然DCAD是评估牧草是否适合奶牛预备日粮的一个重要属性,但目前的数据表明,考虑牧草中钙和其他矿质营养素的浓度是谨慎的,特别是在检查不太熟悉的植物物种或不同培养方式对这些日粮中牧草成分的影响时。
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引用次数: 7
Designing complex research projects to estimate genetic parameters plus treatment and other effects – optimising the experimental design 设计复杂的研究项目来估计遗传参数加上治疗和其他影响——优化实验设计
Pub Date : 2008-08-04 DOI: 10.1071/EA07356
D. L. Robinson
There is an increasing trend towards integrated research, in which several individuals or institutions pool their expertise and make use of common resources, collaborating towards a common set of scientific goals. Integrated research enables a larger number of factors to be investigated, and the most influential or important ones identified, providing information on how the different factors interact or fit together. Good experimental design is, however, required to ensure the aims can be achieved and resources spent wisely. Issues involved in the experimental design of the Australian Beef Cattle Cooperative Research Centre for Meat Quality are discussed. Theoretical results and simulation studies were used to determine optimal numbers of progeny per sire for estimating genetic parameters. For heritabilities of 0.2 and 0.5, the optima are respectively 21 and 9 progeny with recorded measurements. The curves surrounding the optima are quite flat, so aiming for 10–15 progeny with measurements per trait should provide reasonable accuracy in many situations. Estimates of heritabilities, genetic correlations and phenotypic variances have lower sampling correlations than genetic variances and covariances, suggesting that when results are pooled over different breeds or trials, it is better to pool estimates of heritabilities and genetic correlations than (co)variances. Using sires in more than one year increases the robustness of estimated sire effects and increases the accuracy of genetic parameter estimates for hard-to-measure traits (e.g. feed efficiency) that are not recorded on all animals. Unless sires can be chosen as a true random sample of the population, arrangements of link sires (and other effects such as treatments) should be chosen to provide accurate estimates when all terms in the model are fitted as fixed.
综合研究的趋势正在增加,在这种趋势中,几个个人或机构汇集他们的专门知识并利用共同资源,为一套共同的科学目标而合作。综合研究能够调查更多的因素,并确定最具影响力或最重要的因素,提供关于不同因素如何相互作用或相互配合的信息。然而,需要良好的实验设计来确保目标的实现和资源的合理使用。讨论了澳大利亚肉牛肉质合作研究中心实验设计中涉及的问题。理论结果和模拟研究用于确定每一种品种的最佳子代数,以估计遗传参数。遗传力为0.2和0.5时,有记录的最优子代分别为21个和9个。围绕最优点的曲线是相当平坦的,因此在许多情况下,针对每个性状测量10-15个子代应该提供合理的精度。遗传力、遗传相关性和表型方差的估计值比遗传方差和协方差具有更低的抽样相关性,这表明当汇总不同品种或试验的结果时,汇总遗传力和遗传相关性估计值比(co)方差更好。在一年以上的时间内使用母猪增加了估计母猪效应的稳健性,并提高了对难以测量的性状(如饲料效率)的遗传参数估计的准确性,这些性状并非在所有动物身上都有记录。除非可以选择样本作为人口的真正随机样本,否则当模型中的所有项都被拟合为固定时,应该选择链接样本的安排(以及其他影响,如处理)来提供准确的估计。
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引用次数: 2
Application of rumen microbial genome information to livestock systems in the postgenomic era 后基因组时代瘤胃微生物基因组信息在家畜系统中的应用
Pub Date : 2008-06-18 DOI: 10.1071/EA07408
G. Attwood, W. Kelly, E. Altermann, C. Moon, S. Leahy, A. Cookson
Sequencing the genomes of individual rumen microbes and determining the function of their encoded genes promises to transform our understanding of the microbiology of the rumen. The diversity and density of microbes in the rumen, and our inability to culture the majority of rumen microbes, limit current genome studies to only a small fraction of the microbes present in this environment. Nevertheless, genomes of fibre-degrading organisms are beginning to reveal a previously unexpected abundance of genes encoding glycosyl hydrolases and carbohydrate esterases, which could be used to enhance fibre digestion in the rumen. Additionally, genome sequencing of a rumen methanogen is identifying conserved genes within the methanogenic archaea that may serve as targets for their inhibition and therefore reduction of methane emissions from ruminants. The problem of rumen microbe culturability can be overcome by a new approach called metagenomics, in which microbial DNAs are extracted from rumen samples and sequenced independent of cultivation. In the future, sequencing individual genomes and metagenomic libraries is likely to capture much more of the microbial DNA in the rumen and, coupled with postgenomic studies on gene and protein expression, is likely to enhance our knowledge of the microbial component of ruminant digestion.
对单个瘤胃微生物的基因组进行测序并确定其编码基因的功能有望改变我们对瘤胃微生物学的理解。瘤胃中微生物的多样性和密度,以及我们无法培养大多数瘤胃微生物,限制了目前的基因组研究仅局限于这种环境中存在的一小部分微生物。然而,纤维降解生物的基因组开始揭示先前意想不到的编码糖基水解酶和碳水化合物酯酶的基因丰富,这可以用来增强瘤胃中的纤维消化。此外,瘤胃产甲烷菌的基因组测序正在鉴定产甲烷古菌中的保守基因,这些基因可能作为抑制反刍动物甲烷排放的靶点。瘤胃微生物可培养性的问题可以通过一种称为宏基因组学的新方法来克服,该方法从瘤胃样本中提取微生物dna并独立于培养进行测序。在未来,个体基因组测序和宏基因组文库可能会捕获更多的瘤胃微生物DNA,再加上基因和蛋白质表达的后基因组研究,可能会增强我们对反刍动物消化微生物成分的了解。
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引用次数: 15
In utero effects on livestock muscle development and body composition 子宫内对家畜肌肉发育和体成分的影响
Pub Date : 2008-06-18 DOI: 10.1071/EA08017
J. Brameld, Z. Daniel
This review will focus on the evidence for in utero effects on development of skeletal muscle in farm and laboratory animals, particularly sheep and pigs. We will describe genetic and environmental factors that have been shown to alter the numbers of muscle fibres formed and outline our working hypothesis for the mechanism involved and the critical window during pregnancy when these effects are seen. We will then discuss the long-term consequences in terms of body composition. Although this review concentrates on skeletal muscle development, the mechanism we suggest might be equally applicable to other tissues in the body (e.g. the brain, kidneys or sex organs) and, therefore, impact on their physiological functions.
这篇综述将集中在子宫内对农场和实验动物,特别是羊和猪的骨骼肌发育的影响的证据。我们将描述遗传和环境因素,这些因素已被证明会改变形成的肌肉纤维的数量,并概述我们对所涉及的机制的工作假设,以及孕期看到这些影响的关键窗口期。然后我们会讨论身体组成方面的长期后果。虽然这篇综述集中在骨骼肌的发育上,但我们提出的机制可能同样适用于身体的其他组织(如大脑、肾脏或性器官),因此对它们的生理功能有影响。
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引用次数: 24
The effect of finishing diet and meat cuts on the attributes of four processed products from bull beef 肥育日粮和切肉对4种牛肉制品性状的影响
Pub Date : 2008-06-18 DOI: 10.1071/EA07395
M. Farouk, C. Podmore, C. Boom, G. Sheath
Breakfast beef, stir-fry, pot-roast and oven-roast meats were prepared from the brisket, clod, inside round and knuckle of bulls on two pasture-based finishing diets (6 weeks of grain supplement before slaughter or no supplement). Diet affected the fatness of the raw meat but had no significant effect on the attributes assessed by the in-house consumer or trained panel. Stir fry from brisket, and pot roast and oven roast from brisket and clod, had higher (P knuckle > inside round > brisket; and that of product acceptability with appropriate processing was breakfast beef > oven roast > pot roast > stir fry. Female in-house consumer panellists assigned lower acceptability scores to the beef products than their male counterparts (P < 0.05). Because none of the muscles or products made from the muscles were totally rejected by the consumer panel (overall scores range from 4.2 to 7.0), there is potential to add value to these cuts through further processing.
早餐牛肉、炒牛肉、炖牛肉和烤箱烤肉分别来自牛的胸肉、肉、牛的内脏和牛的指关节,采用两种基于牧场的育肥日粮(屠宰前补充6周谷物或不补充谷物)。饮食会影响生肉的脂肪度,但对内部消费者或培训小组评估的属性没有显著影响。从牛腩炒,锅烤和烤箱烤从牛腩和泥,有更高的(P指关节>内圆>牛腩;加工工艺适宜的产品可接受度为早餐牛肉>烤箱烤>锅烤>炒。女性内部消费者小组成员对牛肉产品的可接受性评分低于男性小组成员(P < 0.05)。因为没有肌肉或由肌肉制成的产品被消费者小组完全拒绝(总分从4.2到7.0不等),因此有可能通过进一步加工来增加这些切割的价值。
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引用次数: 0
Development and validation of a simple technique for logging body temperature in free-ranging cattle 开发和验证一种简单的技术来记录自由放养的牛的体温
Pub Date : 2008-06-18 DOI: 10.1071/EA07422
J. Lea, D. Niemeyer, M. Reed, A. Fisher, D. Ferguson
This series of experiments was undertaken to develop a low animal impact, inexpensive and simple method of continuous logging of body temperature in free-ranging adult cattle for periods up to 7 days. Two different types of logger (iButton and Vemco minilogger) and three different methods of attachment (surgical implant, anal girth strap and anal tail attachment) for the iButton were investigated. The technique based on the iButton temperature logger, secured to the tail head was the most effective. This technique required minimal animal handling and provided reliable and accurate measurement of temperature in freely behaving cattle.
进行这一系列实验是为了开发一种低动物影响、廉价和简单的方法,在长达7天的时间内连续记录自由放养的成年牛的体温。研究了iButton的两种不同类型的记录仪(iButton和Vemco迷你记录仪)和三种不同的附着方法(手术植入、肛门环带和肛门尾附着)。基于iButton温度记录仪的技术,固定在尾头是最有效的。这项技术需要最小的动物操作,并提供可靠和准确的温度测量自由行为的牛。
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引用次数: 25
Genotype and age effects on sheep meat production. 5. Lean meat and fat content in the carcasses of Australian sheep genotypes at 20-, 30- and 40-kg carcass weights 基因型和年龄对羊肉产量的影响。5. 20、30和40公斤胴体体重时澳大利亚绵羊基因型胴体的瘦肉和脂肪含量
Pub Date : 2008-06-18 DOI: 10.1071/EA08054
E. Ponnampalam, K. Butler, D. Hopkins, M. Kerr, F. Dunshea, R. Warner
Leanmeatand fat content of Australiansheep genotypes were comparedat20-,30- and 40-kg carcass weights. Sheep comprised Poll Dorsetgrowth ·Border Leicester Merino (PDg ·BLM), Poll Dorsetgrowth ·Merino (PDg ·M), Poll Dorsetmuscling ·Merino (PDm ·M), Border Leicester ·Merino (BL ·M) and Merino ·Merino (M ·M) genotypes. Lambs wereraisedasamixedflockundergrazingandslaughteredat4,8,14and22monthsofagewitheachslaughtertimeinvolving ~150mixed sex animals. At 24h after slaughter, chilled carcasses were halved along the backbone and the right sides were usedfordeterminationoflean,fatandashpercentagesusingdualenergyX-rayabsorptiometry.Withinaparticularagegroup andgenotype,animalsgrowingatfasterratesandreachingheaviercarcassweightshadlowercarcassleanmeatcontentthan slowergrowinganimals.Merinocarcassesweighing20and30kghadsimilarlevelsofleanmeattoPD ·Mgenotypes,which wasgreaterthanthatfromtheBL ·Mgenotype.Second-crossPD ·BLMcarcassesweighing20kgat4monthsand30kgat 8monthshadsimilarcarcassfatandleanpercentagesto20-kgMerinocarcassesat8monthsandfirst-crossPD ·Mcarcasses weighing30kgat14 months, respectively. At40-kgcarcass weight, 22-month-oldMerinoshad similarlevelsofleannessto carcasses from 22-month-old PD ·M animals and carcasses from 14-month-old second-cross PD ·BLM animals. Carcass leanmeatcontentdecreasedwithincreasingcarcassweightandfirst-crossBL ·Manimalshadthelowestcarcassleanacross allweightcategories.Therewasamajoraccelerationincarcassfatnessbetween14and22monthsassociatedwithareduction inmuscle deposition.Results indicatethatage ofthe animalshouldbe taken intoaccount whencarcass leanand fat contents are compared at a particular carcass weight. Merinos will achieve weight/composition specifications at least equally well to crossbreds but will take longer with a likely increase in production costs.
比较了20、30和40公斤胴体重时澳大利亚绵羊基因型的瘦肉和脂肪含量。绵羊包括Poll多赛特生长·边境莱斯特美利奴(PDg·BLM)、Poll多赛特生长·美利奴(PDg·M)、Poll多赛特肌肉·美利奴(PDm·M)、边境莱斯特·美利奴(BL·M)和美利奴·美利奴(M·M)基因型。羔羊分别饲养于4、8、14、22个月大的混交羊群中,屠宰时间约150只。屠宰后24小时,将冷却后的胴体沿脊椎骨切成两半,用双能x射线吸收仪测定胴体右侧的瘦肉、脂肪和脂肪百分比。在特定的种群和基因型中,与生长较慢的动物相比,生长速度更快、胴体重量更重、胴体重量更低、肉含量更低的动物。体重为20公斤和30公斤的merinocarassa的leinep·m基因型水平相似,高于bl·m基因型。第二次交叉spd·blmccars4个月重20公斤,第30公斤重8个月重20公斤,第一次交叉spd·mccars8个月重30公斤,第一次交叉spd·mccars14个月重30公斤。在胴体体重40公斤时,22月龄美利奴与22月龄PD·M动物和14月龄二交PD·BLM动物的胴体具有相似的瘦度。胴体瘦肉含量随着胴体体重的增加而降低,初交种动物的瘦肉含量在所有体重类别中都最低。在14至22个月之间,脂肪的增加与肌肉沉积的减少有关。结果表明,在比较特定胴体重量下的胴体瘦脂肪含量时,应考虑动物的肥力。美利奴将达到重量/成分规格,至少与杂交品种一样好,但需要更长时间,生产成本可能会增加。
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引用次数: 33
Manipulating dietary preference to improve animal performance 操纵饮食偏好以提高动物生产性能
Pub Date : 2008-06-18 DOI: 10.1071/EA08006
G. Edwards, A. Parsons, R. Bryant
This paper reviews opportunities to improve the diet quality, intake and performance of animals through manipulation of the partial preference commonly shown by grazing animals for different pasture components. Using evidence from the well-worked example of grass–legume pastures, potential opportunities to manipulate preference are highlighted, including: (i) altering plant characteristics to change the relative preference of alternative species or cultivars; (ii) utilising variation in preference among and within animal species; and (iii) working with, rather than against, known preference patterns, by offering forages as spatially separated monocultures within the same paddock or at different times of the day. In all cases, it is argued that is important to consider the full complexities of pasture–animal interactions, in particular, how current diet choices feed back to determine choices available subsequently in the pasture. To develop feeding systems where desirable pasture traits for animal performance are sustained at a high abundance in the diet, plant and animal breeding selection practises and grazing management systems should take greater account of the existence of partial preferences.
本文综述了通过控制放牧动物对不同牧草成分的部分偏好来改善动物日粮质量、采食量和生产性能的机会。利用从草-豆科牧草的良好案例中获得的证据,强调了操纵偏好的潜在机会,包括:(i)改变植物特性以改变替代物种或栽培品种的相对偏好;(ii)利用动物物种之间和物种内部的偏好差异;(三)利用而不是反对已知的偏好模式,在同一围场内或一天的不同时间提供空间分离的单一栽培饲料。在所有情况下,认为重要的是要考虑牧场与动物相互作用的全部复杂性,特别是当前的饮食选择如何反馈,以确定随后在牧场中可用的选择。为了发展饲料中对动物生产性能有利的牧草特性保持高丰度的喂养系统,植物和动物育种选择实践和放牧管理系统应更多地考虑到部分偏好的存在。
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引用次数: 24
Application of gene expression studies in livestock production systems: a European perspective 基因表达研究在畜牧生产系统中的应用:欧洲视角
Pub Date : 2008-06-18 DOI: 10.1071/EA08018
I. Cassar-Malek, B. Picard, C. Bernard, J. Hocquette
In the context of sustainable agriculture and animal husbandry, understanding animal physiology remains a major challenge in the breeding and production of livestock, especially to develop animal farming systems that respond to the new and diversified consumer demand. Physiological processes depend on the expression of many genes acting in concert. Considerable effort has been expended in recent years on examining the mechanisms controlling gene expression and their regulation by biological and external factors (e.g. genetic determinants, nutritional factors, and animal management). Two main strategies have been developed to identify important genes. The first one has focussed on the expression of candidate genes for key physiological pathways at the level of both the transcripts and proteins. An original strategy has emerged with the advent of genomics that addresses the same issues through the examination of the molecular signatures of all genes and proteins using high-throughput techniques (e.g. transcriptomics and proteomics). In this review, the application of the gene expression studies in livestock production systems is discussed. Some practical examples of genomics applied to livestock production systems (e.g. to optimise animal nutrition, meat quality or animal management) are presented, and their outcomes are considered. In the future, integration of the knowledge gained from these studies will finally result in optimising livestock production systems through detection of desirable animals and their integration into accurate breeding programs or innovative management systems.
在可持续农业和畜牧业的背景下,了解动物生理学仍然是牲畜育种和生产中的一个主要挑战,特别是在开发能够满足新的和多样化消费者需求的动物养殖系统方面。生理过程依赖于许多协同作用的基因的表达。近年来,人们在研究基因表达的控制机制及其受生物和外部因素(如遗传决定因素、营养因素和动物管理)的调控方面付出了相当大的努力。已经发展了两种主要的策略来识别重要的基因。第一个集中在转录本和蛋白质水平上的关键生理途径的候选基因的表达。随着基因组学的出现,通过使用高通量技术(例如转录组学和蛋白质组学)检查所有基因和蛋白质的分子特征来解决相同问题的原始策略已经出现。本文综述了基因表达研究在畜牧生产系统中的应用。介绍了基因组学应用于牲畜生产系统的一些实际例子(例如,优化动物营养、肉质或动物管理),并考虑了它们的结果。在未来,整合从这些研究中获得的知识将最终通过发现理想的动物并将其整合到准确的育种计划或创新的管理系统中来优化畜牧生产系统。
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引用次数: 46
期刊
Australian Journal of Experimental Agriculture
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