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Metabolic profile and oxidative status in goats during the peripartum period 围产期山羊的代谢特征和氧化状态
Pub Date : 2008-06-18 DOI: 10.1071/EA07410
P. Celi, A. Trana, A. Quaranta
The aim of this study was to monitor the metabolic profile and oxidative status in goats during the peripartum period. A blood sample was taken from 10 Red Syrian goats on days −21, −3, +1, +14, and +28 from delivery. Samples were assayed for glutathione peroxidase and superoxide dismutase activities, and reactive oxygen metabolites, insulin, insulin-like growth factor-I, free triiodothyronine (fT3), free thyroxine (fT4), leptin, urea, non-esterified fatty acids, triglycerides, cholesterol, total protein, albumin, globulin, calcium and inorganic phosphorus concentrations. Blood glutathione peroxidase activity decreased during the postpartum period and its values were significantly (P < 0.05) lower on days 14 and 28 postpartum. Albumin levels were significantly (P < 0.05) lower on days −3, 1 and 28 from delivery compared with day −21. Plasma urea levels significantly (P < 0.001) decreased starting from day −3 from delivery. No effect of time from delivery was noted on reactive oxygen metabolites, non-esterified fatty acids, triglycerides, cholesterol, total protein, calcium and inorganic phosphorus, insulin and leptin concentrations and superoxide dismutase activity. Plasma concentrations of fT3 were significantly (P < 0.01) higher on days 14 and 28 compared with days −21, −3 and 1 from delivery. Plasma levels of fT4 were significantly (P < 0.01) lower on days −3 and 1 from delivery. Plasma insulin-like growth factor-I values slowly decreased during the postpartum period and its values were significantly (P < 0.05) lower on day 28. This study indicates that goats experienced moderate oxidative stress during the peripartum period.
本研究的目的是监测围生期山羊的代谢特征和氧化状态。10只叙利亚红山羊于分娩后- 21、- 3、+1、+14和+28天采集血样。检测样品谷胱甘肽过氧化物酶和超氧化物歧化酶活性、活性氧代谢物、胰岛素、胰岛素样生长因子- 1、游离三碘甲状腺原氨酸(fT3)、游离甲状腺素(fT4)、瘦素、尿素、非酯化脂肪酸、甘油三酯、胆固醇、总蛋白、白蛋白、球蛋白、钙和无机磷浓度。产后血谷胱甘肽过氧化物酶活性降低,产后14、28 d血谷胱甘肽过氧化物酶活性显著降低(P < 0.05)。与分娩后第21天相比,分娩后第3,1和28天白蛋白水平显著降低(P < 0.05)。血浆尿素水平从分娩后第3天开始显著下降(P < 0.001)。分娩时间对活性氧代谢物、非酯化脂肪酸、甘油三酯、胆固醇、总蛋白、钙和无机磷、胰岛素和瘦素浓度以及超氧化物歧化酶活性没有影响。与分娩后第21天、第3天和第1天相比,分娩后第14天和第28天血浆fT3浓度显著升高(P < 0.01)。分娩后第3天和第1天血浆fT4水平显著降低(P < 0.01)。血浆胰岛素样生长因子- 1在产后缓慢下降,并在第28天显著降低(P < 0.05)。本研究表明,山羊在围产期经历中度氧化应激。
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引用次数: 58
Linking rumen function to animal response by application of metagenomics techniques 应用宏基因组技术将瘤胃功能与动物反应联系起来
Pub Date : 2008-06-18 DOI: 10.1071/EA08028
J. Firkins, S. Karnati, Zhongtang Yu
Metagenomics techniques applied to the rumen microbiota have demonstrated tremendous diversity originally among populations of bacteria and, more recently, among the methanogenic archaea, including those associated with protozoa. Although with some potential limitations, cluster analyses of sequences recovered from clone libraries have revealed differences in populations among animals fed forage v. grain, including amylolytic ruminococci and novel groups of clostridia adhering to the rumen particulates. Rapid profiling procedures, such as denaturing gradient gel electrophoresis (DGGE), can be used to infer likely differences in community structure of bacteria and archaea among numerous replicates of animals and times after feeding diets that are more representative of intense ruminant animal production. Metagenomics procedures also are being applied to issues related to ruminal output of fatty acid isomers influencing milk fat composition and consumer acceptance, the environmental impact of nitrogen in animal waste and methane emissions, and future potential approaches to improve ruminal fibre digestibility. If varying concentrations of ruminal metabolites and fluxes quantified from microbial processes can be combined with results from metagenomics applied to rumen microbiota, then we should reduce the unexplained variability in models in which the prediction of nutrient supply to the intestine is synchronised with nutritional guidelines for more efficient feed conversion by ruminants.
应用于瘤胃微生物群的宏基因组学技术表明,最初在细菌种群中存在巨大的多样性,最近在产甲烷的古菌中,包括与原生动物相关的古菌。尽管存在一些潜在的局限性,但从克隆文库中恢复的序列聚类分析已经揭示了饲料和谷物饲料动物群体的差异,包括解淀粉性瘤胃球菌和粘附在瘤胃颗粒上的新型梭菌群。快速分析程序,如变性梯度凝胶电泳(DGGE),可用于推断细菌和古菌群落结构在多次重复的动物和饲喂更能代表强烈反刍动物生产的饲料后的时间之间的可能差异。元基因组学程序也被应用于与影响乳脂组成和消费者接受度的脂肪酸异构体的瘤胃产量、动物粪便中氮和甲烷排放的环境影响以及未来提高瘤胃纤维消化率的潜在方法有关的问题。如果从微生物过程中量化的不同浓度的瘤胃代谢物和通量可以与应用于瘤胃微生物群的宏基因组学结果相结合,那么我们应该减少模型中无法解释的变异,在这些模型中,对肠道营养供应的预测与反刍动物更有效的饲料转化的营养指南同步。
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引用次数: 28
Improving calf survival and performance by supplementation in Bali cattle 通过补充饲料提高巴厘牛犊牛存活率和生产性能
Pub Date : 2008-06-18 DOI: 10.1071/EA08038
I. Jelantik, M. Mullik, C. L. Leo-Penu, J. Jeremias, R. Copland
An on-farm experiment was conducted in several villages in the district of Kupang, East Nusa Tenggara Province (NTT), Indonesia from June to October 2007. The experiment investigated the efficacy of a supplementation strategy to improve calf survival and performance during the dry season. A total of 258 Bali (Banteng, Bos javanicus) cow-calf pairs were used in the experiment. Calves from the 190 cow-calf pairs in the treatment groups were allocated grass hay + concentrate supplementation as follows: 1% (n = 98), 2% (n = 56) and 3% (n = 42) of calf bodyweight. There were 62 cow-calf pairs in the unsupplemented control group. The supplement consisted of grass hay and concentrate (rice bran, cornmeal, leucaena leaf and fish meal) containing 18% crude protein. The supplement was introduced to calves in the morning while confined to calf pens when the dams were grazing. Calves were reunited with the cows during the night. Parameters measured were calf mortality, calf and cow daily gain, and milk production. Supplementation tended to reduce calf mortality (P = 0.094) from 6.4% in control to 0% in calves receiving 2% and 3% supplement. Levels of supplementation significantly (P < 0.001) improved calf daily weight gain. Bodyweight changes and milk production of cows were unaffected by calf supplementation. It can be concluded that supplementation at 2% calf bodyweight reduces calf mortality and improves calf weight gain.
2007年6月至10月在印度尼西亚东努沙登加拉省(NTT) Kupang区的几个村庄进行了一项农场试验。本试验旨在研究一种添加策略对提高旱季犊牛成活率和生产性能的效果。试验共选用258对巴厘(Banteng, Bos javanicus)牛犊牛。各处理组190对犊牛分别按犊牛体重的1% (n = 98)、2% (n = 56)和3% (n = 42)添加干草+精料。对照组为62对母牛。添加物为含18%粗蛋白质的干草和精料(米糠、玉米粉、合家乐叶和鱼粉)。在早上给小牛喂食补充剂,而当水坝放牧时将其限制在小牛围栏内。小牛在夜间与母牛团聚。测量的参数是小牛死亡率、小牛和奶牛日增重以及产奶量。犊牛死亡率(P = 0.094)从对照组的6.4%降低到添加2%和3%的犊牛的0%。饲粮添加水平显著提高了犊牛日增重(P < 0.001)。奶牛的体重变化和产奶量不受补充小牛的影响。由此可见,在犊牛体重的2%水平上添加饲料可降低犊牛死亡率,提高犊牛增重。
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引用次数: 13
Diversity of methanogens in ruminants in Queensland 昆士兰反刍动物产甲烷菌的多样性
Pub Date : 2008-06-18 DOI: 10.1071/EA08049
D. Ouwerkerk, A. F. Turner, A. Klieve
Methane emissions from ruminant livestock represent a loss of carbon during feed conversion, which has implications for both animal productivity and the environment because this gas is considered to be one of the more potent forms of greenhouses gases contributing to global warming. Many strategies to reduce emissions are targeting the methanogens that inhabit the rumen, but such an approach can only be successful if it targets all the major groups of ruminant methanogens. Therefore, a thorough knowledge of the diversity of these microbes in different breeds of cattle and sheep, as well as in response to different diets, is required. A study was undertaken using the molecular techniques denaturing gradient gel electrophoresis, DNA cloning and DNA sequence analysis to define the extent of diversity among methanogens in ruminants, particularly Bos indicus cross cattle, on differing forages in Queensland. It was found that the diversity of methanogens in forage-fed cattle in Queensland was greater than in grain-fed cattle but there was little variability in methanogen community composition between cattle fed different forages. The species that dominate the rumen microbial communities of B. indicus cross cattle are from the genus Methanobrevibacter, although rumen-fluid inoculated digestors fed Leucaena leucocephala leaf were populated with Methanosphaera-like strains, with the Methanobrevibacter-like strains displaced. If ruminant methane emissions are to be reduced, then antimethanogen bioactives that target both broad groups of ruminant methanogens are most likely to be needed, and as a part of an integrated suite of approaches that redirect rumen fermentation towards other more useful end products.
反刍牲畜的甲烷排放代表了饲料转化过程中碳的损失,这对动物生产力和环境都有影响,因为这种气体被认为是导致全球变暖的更有效的温室气体之一。许多减少排放的策略都是针对瘤胃中的产甲烷菌,但这种方法只有针对所有主要的反刍动物产甲烷菌群才能成功。因此,需要全面了解这些微生物在不同品种的牛羊身上的多样性,以及它们对不同饮食的反应。利用分子变性梯度凝胶电泳、DNA克隆和DNA序列分析等技术,研究了昆士兰不同牧草上反刍动物(特别是杂交牛)产甲烷菌的多样性程度。结果表明,昆士兰草饲牛产甲烷菌群落多样性高于粗粮饲牛,但不同草饲牛产甲烷菌群落组成差异不大。在杂交牛瘤胃微生物群落中占主导地位的菌种是甲烷预防菌属,但在瘤胃液接种过的消化器中,以白毛藻叶为饲料的瘤胃中存在类似甲烷菌的菌种,而类似甲烷预防菌的菌种被取代。如果要减少反刍动物的甲烷排放,那么最有可能需要针对反刍动物产甲烷菌的广泛群体的抗产甲烷菌生物活性物质,并且作为将瘤胃发酵转向其他更有用的最终产品的综合方法的一部分。
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引用次数: 29
Approaches for assessing some attributes of feed-base systems in mixed farming enterprises 混合养殖企业饲料基础系统某些属性的评估方法
Pub Date : 2008-06-18 DOI: 10.1071/EA07421
L. Bell, M. Robertson, D. Revell, J. Lilley, A. Moore
Improving the feed-base to better balance livestock demands with the variation in feed supply can improve the sustainability and productivity of livestock enterprises. This paper outlines some approaches and tools that can be applied to the assessment of new feed options and/or potential changes to the feed-base in mixed farming systems. Demonstrations of strategic aspects of designing feed systems include: whole-farm feed planning using simple tools, such as the MLA Feed Demand Calculator, that enable iterative changes to the balance between feed supply and demand to be considered simultaneously; assessing production and environmental risks of different feed-base systems using simulation models (e.g. APSIM, GRAZPLAN); and using bio-economic models (e.g. MIDAS) to investigate the impact of a new feed source on whole-farm profitability and the optimal balance of other feed sources and livestock production system. Also included is an example of an approach to identifying opportunities and seasonal triggers for a tactical response for utilising an alternate feed source (e.g. grazing a grain crop). The importance of economics and risk as factors for assessing feed-base systems is demonstrated. In particular, the marginal value of extra feed supply is a critical element driving the whole-farm economics of the feed system. Some approaches consider seasonal averages, but the risk of year-to-year and within-year variations in the timing and amount of feed supply should also be assessed. Several tools of varying complexity exist to investigate attributes of the feed-base, but it is important that the correct approach is applied to the particular question in mind. A range of approaches could be applied concurrently to fully explore a range of aspects of the performance of a feed-base system.
改善饲料基础,更好地平衡牲畜需求和饲料供应的变化,可以提高畜牧企业的可持续性和生产力。本文概述了可用于评估混合养殖系统中新饲料选择和/或饲料基础潜在变化的一些方法和工具。设计饲料系统的战略方面的演示包括:使用简单的工具进行整个农场饲料规划,例如MLA饲料需求计算器,它可以同时考虑饲料供需平衡的迭代变化;利用模拟模型(如APSIM、GRAZPLAN)评估不同饲料基础系统的生产和环境风险;并使用生物经济模型(例如MIDAS)调查新饲料来源对整个农场盈利能力的影响以及其他饲料来源和牲畜生产系统的最佳平衡。还包括一个方法示例,以确定利用替代饲料来源(例如放牧谷物作物)的战术反应的机会和季节性触发因素。经济和风险作为评估饲料基系统的因素的重要性得到了证明。特别是,额外饲料供应的边际价值是推动饲料系统的整个农场经济的关键因素。有些方法考虑季节平均值,但也应评估饲料供应时间和数量的年与年之间和年内变化的风险。有几种不同复杂程度的工具可以用来研究feed-base的属性,但重要的是要将正确的方法应用于所考虑的特定问题。可以同时应用一系列方法来充分探索馈基系统性能的一系列方面。
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引用次数: 33
Effect of whole cottonseed supplementation on energy and nitrogen partitioning and rumen function in dairy cattle on a forage and cereal grain diet 饲粮中添加全棉籽对饲粮和谷物型奶牛能量、氮分配及瘤胃功能的影响
Pub Date : 2008-06-18 DOI: 10.1071/EA07400
C. Grainger, T. Clarke, R. Eckard
The experimental objective was to determine the effect of adding whole cottonseed (WCS) to a forage and cereal grain diet on the energy and nitrogen balance and rumen function of lactating dairy cattle. Two experiments were carried out, a field experiment and an indoor metabolism experiment. In the field experiment, 50 lactating cows ~200 days in milk were randomly allocated to one of two groups (control or WCS). Cows were offered lucerne hay (morning) and ryegrass-based pasture silage (afternoon) in one group for 5 weeks. The hay and silage were placed on the ground in a bare paddock. Cows in each group were also individually offered cracked grain in a feed trough at 3 kg dry matter (DM)/cow.day at milking times. In addition, at milking times, cows in the WCS group were individually offered 2.7 kg DM/cow.day of WCS with their grain supplement. Samples of rumen fluid were collected from each fistula, ~4 h after grain feeding in the morning, of eight cows (four per group) on 1 day in each of the 5 weeks of treatment. In the metabolism experiment, immediately after the 5 weeks of feeding, 12 lactating cows, six from each treatment from the field experiment, were randomly selected and individually housed in metabolism stalls and fed the same diets for a 6-day energy and nitrogen balance study. Cows were fed at milking times (0700 and 1530 hours) and all feed offered and refused was weighed daily. All cows were offered 5.6 kg DM/cow.day of pasture silage, 4 kg DM/cow.day of lucerne hay and 3 kg DM/cow.day of cereal grain. In addition, cows in the WCS treatment group were offered 2.7 kg DM/cow.day of WCS with their grain supplement. In the metabolism study, adding WCS to the diet resulted in a greater energy intake, but there was no depression in energy digestibility. Whole cottonseed also increased nitrogen intake and nitrogen digestibility of the diet was increased from 62 to 75%, but the proportion of nitrogen in milk remained the same with a greater proportion of nitrogen appearing in body tissue. In the field experiment, supplementation with WCS did not alter rumen fluid ammonia-N or volatile fatty acid concentrations. Adding WCS did not affect three of the main classes of protozoa, based on size, within the two major orders of ciliate protozoa. The WCS did, however, reduce the levels of entodiniomorphs >200 µm diameter and holotrichs < 200 µm diameter, but these only account for a small number of the total protozoa present. Supplementation of a forage and grain-based diet with WCS improved the energy and protein content of the diet without any negative effects on rumen digestion and with a similar proportion of dietary energy and nitrogen appearing in milk. Over the summer period in winter-rainfall dairying areas in south-east Australia when pasture availability is limited and the diet is mainly comprised of forage and cereal grain fed at a level that is energy-limiting for maximum production, WCS can be and is used to supplement the diet to imp
本试验旨在研究饲粮和谷物饲粮中添加全棉籽对泌乳奶牛能量氮平衡及瘤胃功能的影响。进行了田间试验和室内代谢试验。田间试验选用50头泌乳~200日龄奶牛,随机分为对照组和WCS组。每组饲喂紫花苜蓿干草(上午)和黑麦草类牧草青贮(下午),连续饲喂5周。干草和青贮饲料放在光秃秃的围场的地上。每组奶牛分别饲喂干物质(DM)为3 kg /头的碎粒饲料。每天挤奶的时候。此外,在挤奶时,WCS组奶牛每头单独饲喂2.7 kg干物质。在WCS中添加谷物。8头奶牛(每组4头)在5周的治疗中,每组1天,于晨饲后4 h从每个瘘管处采集瘤胃液。代谢试验中,在饲喂5周后,随机选取12头泌乳奶牛,每组6头,单独饲养于代谢栏内,饲喂相同的饲粮,进行为期6 d的能量氮平衡试验。在挤奶时间(0700和1530小时)饲喂奶牛,每天称重所有供饲和拒绝的饲料。每头奶牛饲喂5.6 kg DM。日牧草青贮,每头牛4 kg DM。每头牛饲喂3 kg干草料。谷物的日子。WCS处理组每头奶牛饲喂2.7 kg干物质。在WCS中添加谷物。在代谢研究中,饲粮中添加WCS会增加能量摄入,但不会降低能量消化率。全棉籽也提高了饲粮氮消化率,使饲粮氮消化率从62%提高到75%,但牛奶中氮的比例保持不变,身体组织中氮的比例更高。在田间试验中,添加WCS对瘤胃液氨氮和挥发性脂肪酸浓度没有影响。在纤毛虫原生动物的两个主要目中,根据大小,添加WCS对三个主要类别的原生动物没有影响。然而,WCS确实降低了直径小于200µm的内二胚和直径小于200µm的holotrichs的水平,但这些只占总原生动物的一小部分。在饲料和谷物基础饲粮中添加WCS可提高饲粮的能量和蛋白质含量,但对瘤胃消化没有负面影响,且牛奶中出现的饲粮能量和氮的比例相似。在澳大利亚东南部冬季降雨奶牛区的夏季,牧草供应有限,饲粮主要由饲料和谷物组成,饲料的能量水平限制了最大产量,WCS可以而且已经用于补充饲粮,以提高产奶量和盈利能力。
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引用次数: 4
Higher weaning weight improves postweaning growth and survival in young Merino sheep 较高的断奶重可提高美利奴羊幼羊断奶后的生长和存活率
Pub Date : 2008-06-18 DOI: 10.1071/EA07407
S. Hatcher, J. Eppleston, R. Graham, J. Mcdonald, S. Schlunke, B. Watt, K. Thornberry
Two monitoring projects were conducted to investigate weaner mortality in commercial Merino flocks in the Yass and the Central Tablelands Rural Lands Protection Boards located in the Southern Tablelands agricultural region of New South Wales. The projects were conducted in Yass in 2005 and in the Central Tablelands in 2006. A random sample of weaners from four flocks in the Yass board and 11 flocks in the Central Tablelands board were regularly weighed, growth rates were calculated after weaning and survival was determined by the continuing presence of an individual weaner at subsequent weighing activities. Weaning weight was the most important factor in determining postweaning liveweight, growth rates and survival with the significant impact of weaning weight on liveweight persisting for up to 6 months after weaning. Despite the lightest weaners being capable of considerable compensatory growth given sufficient postweaning nutrition, the lightest 25% of weaners were more than twice as likely to die as heavier weaners. A focus on ewe nutrition and parasite control during late pregnancy and lactation will allow Merino producers to achieve higher weaning weights that will set their weaners up for strong postweaning growth with a decreased likelihood of mortality.
在新南威尔士州南部高原农业区的亚斯和中部高原农村土地保护委员会开展了两个监测项目,以调查商业美利奴鸡群的断奶死亡率。这些项目分别于2005年在亚斯和2006年在中央高原地区进行。从亚斯区4个断奶仔猪群和中部高原区11个断奶仔猪群中随机取样,定期称重,计算断奶后的生长率,并通过随后称重活动中单个断奶仔猪的持续存在来确定存活率。断奶重量是决定断奶后活重、生长率和存活率的最重要因素,断奶重量对活重的显著影响持续至断奶后6个月。尽管最轻的断奶仔猪在断奶后营养充足的情况下能够获得相当大的补偿性生长,但最轻的25%断奶仔猪的死亡率是较重断奶仔猪的两倍多。在妊娠后期和哺乳期关注母羊的营养和寄生虫控制将使美利奴牧场主获得更高的断奶体重,这将使他们的断奶仔猪在断奶后强劲生长,降低死亡率的可能性。
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引用次数: 30
Heifer fertility on commercial cattle properties in the Northern Territory 北领地商业牛场的小母牛生育力
Pub Date : 2008-06-18 DOI: 10.1071/EA08057
T. Schatz, M. Hearnden
The performance of heifers at their first (maiden heifers) and second matings (first lactation heifers) was recorded between 2003 and 2008 to establish current heifer fertility levels on 14 commercial cattle properties in the Northern Territory (NT). Pregnancy rates in maiden heifers (joined first at ~2 years of age) were generally adequate (>75%) to produce enough pregnant replacement breeders. Pregnancy rates in first lactation heifers were often very low ( 70%). Calf loss between pregnancy diagnosis and weaning in first lactation heifers was often high (>30%). The average of the calf loss figures from all the herds studied was 22%.
在2003年至2008年期间,记录了北领地(NT) 14个商业养牛场的母牛首次(处女母牛)和第二次交配(第一次哺乳母牛)的表现,以确定当前的母牛生育力水平。雏牛(2岁左右首次加入)的妊娠率通常足够(约75%)以产生足够的妊娠替代种猪。初次哺乳期母牛的妊娠率通常很低(70%)。在首次哺乳的母牛中,从怀孕诊断到断奶之间的小牛损失通常很高(bbb30 %)。所有被研究的象群中牛犊的平均损失数字是22%。
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引用次数: 22
Reduced space allowance for adult sheep in lairage for 24hours limits lying behaviour but not drinking behaviour 成年羊在饲养场24小时内的空间减少,限制了它们的躺卧行为,但不限制它们的饮水行为
Pub Date : 2008-06-18 DOI: 10.1071/EA08039
E. Jongman, M. K. Edge, K. Butler, G. Cronin
The key lairage requirements for livestock include sufficient space to lie down, sufficient time to allow recovery from travel, and access to water to allow recovery from dehydration. Current recommendations for lairage of adult sheep include that holding pens should provide no less space than 0.6 m2 per sheep. Particularly during periods when large numbers of sheep are culled, space allowances may be limited to 0.3 m2 or less, which may reduce the ability of individual sheep to lie down or access water. Adult Merino-cross sheep were allocated to one of four space allowance treatments during unloading from commercial transport vehicles at a commercial abattoir after an average transport time of 3 h. The treatments were 0.3, 0.45, 0.6 and 1.0 m2/sheep and each pen measured 18 m2. Differences in space allowance were achieved by varying the number of sheep per pen, with pens holding 18 (1 m2), 30 (0.6 m2), 40 (0.45 m2) and 60 (0.3 m2) animals. In each pen, eight focal sheep were randomly selected and marked on the back for identification on video records. The sheep remained in the lairage treatments for 24 h and were continuously recorded on video using low light cameras. The data were subsequently analysed for both lying and drinking behaviours after 8 and 24 h in lairage. There were five replicates of all treatments over a 3-week period. A space allowance of 0.3 m2/sheep reduced the time sheep spent lying and the proportion of sheep that lay down at least once, compared with greater space allowances. Space allowances between 0.3 and 1 m2/sheep showed a linear relationship with the time sheep spent lying during the first 24 h. Optimal space allowance may be greater than 1 m2, based on lying behaviour. Drinking behaviour was not affected by space allowance in a 24 h period in lairage although overall 20% of sheep were not observed to drink after 24 h in lairage.
对牲畜的主要饲养要求包括有足够的空间躺下,有足够的时间从旅行中恢复,有足够的水从脱水中恢复。目前对成年羊放养的建议包括,每只羊的围栏空间不少于0.6平方米。特别是在大量羊被扑杀期间,空间可能限制在0.3平方米或更小,这可能会降低单个羊躺下或取水的能力。在平均运输时间为3小时后,在商业屠宰场从商业运输车辆卸载时,将成年美利奴杂交羊分配到四种空间补贴处理中的一种。处理分别为0.3、0.45、0.6和1.0 m2/羊,每圈18 m2。通过改变每个羊圈的羊数来实现空间的差异,羊圈可以容纳18只(1 m2)、30只(0.6 m2)、40只(0.45 m2)和60只(0.3 m2)的羊。在每个围栏中,随机选择8只焦点羊,并在背面标记,以便在视频记录中识别。放养处理24 h后,用弱光摄像机连续录像。随后,研究人员对8小时和24小时后的撒谎和饮酒行为进行了分析。所有处理在3周内进行5次重复。与更大的空间相比,0.3 m2/只羊的空间允许减少了羊躺卧的时间和至少躺一次的羊的比例。0.3 ~ 1 m2/羊的空间余量与羊在头24 h内的躺卧时间呈线性关系。根据躺卧的行为,最佳空间允许可能大于1平方米。在24小时的圈养期间,尽管总体上20%的羊在24小时的圈养后没有观察到喝水,但饮水行为不受空间限制的影响。
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引用次数: 9
Intramuscular fat levels in sheep muscle during growth 绵羊生长期间肌内脂肪水平
Pub Date : 2008-06-18 DOI: 10.1071/EA08046
M. McPhee, D. Hopkins, D. Pethick
A5 ·4 factorial experiment was designed in which lambs representing five genotypes were slaughtered at four ages (110, 236, 412 and 662 days of age). The genotypes represented were Poll Dorsetgrowth ·Border Leicester Merino, Poll Dorsetgrowth ·Merino, Poll Dorsetmuscling ·Merino, Merino ·Merino and Border Leicester ·Merino. Both sexes (ewes and wethers) were represented for each genotype and slaughter age combination. In total, 595 animals were slaughtered and the carcass composition and intramuscular fat were measured. Carcass composition (fat, ash and protein (lean)) was determined by dual energy X-ray absorptiometry, with the intramuscular fat percentage determined using near- infrared spectroscopy following removal and weighing of the entire longissimus thoracis et lumborum (LL) muscle. Analysis revealed that the proportion of intramusular fat in the loin relative to total carcass fat decreases as animals mature, thus indicating that intramusular fat deposition occurs early in the maturation of sheep. Furthermore, as animals became heavier and older the accretion rate of intramuscular fat in the LL muscle slowed down. Both genotype (P < 0.05) and sex (P < 0.001) were found to impact on this pattern, with Border Leicester ·Merino animals exhibiting the largest increase in intramuscular fat proportion in the LL muscle (4.92 and 5.50% at 22 months of age for ewes and wethers, respectively). The Poll Dorsetgrowth ·Border Leicester Merino animals were found to have the greatest absolute levels of intramuscular fat in the whole LL muscle (80.95 and 97.60g at maturity for ewes and wethers, respectively). The amount of intramuscular fat significantly increased as the sheep became older and fatter; however, these differences were quantitatively small. As such, finishing prime lambs to high levels of total carcass fatness would have little effect on any eating quality benefits associated with increased intramuscular fat proportion.
设计5·4因子试验,选取5种基因型的羔羊,分别在4个日龄(110、236、412和662日龄)屠宰。所代表的基因型为Poll多赛特生长·边境莱斯特美利奴、Poll多赛特生长·美利奴、Poll多赛特肌肉·美利奴、美利奴·美利奴和边境莱斯特·美利奴。每种基因型和屠宰年龄组合均代表了两性(母羊和公羊)。共屠宰595只动物,测定胴体成分和肌内脂肪含量。胴体组成(脂肪、灰分和蛋白质(瘦肉))采用双能x射线吸收仪测定,肌内脂肪百分比采用近红外光谱法测定,然后切除并称量整个胸腰最长肌(LL)。分析表明,随着动物的成熟,腰部肌内脂肪相对于胴体总脂肪的比例降低,这表明肌内脂肪沉积发生在绵羊成熟早期。此外,随着动物体重的增加和年龄的增长,左腰肌肌内脂肪的增加速度减慢。基因型(P < 0.05)和性别(P < 0.001)均对这一模式有影响,其中边境莱斯特·美利奴动物的LL肌肌内脂肪比例增加最多,母羊和公羊22月龄时分别增加4.92%和5.50%。结果表明,波尔多塞特生长·边境莱斯特美利奴羊的肌内脂肪绝对含量最高(母羊和公羊成熟时分别为80.95和97.60g)。随着绵羊年龄的增长和体重的增加,肌内脂肪含量显著增加;然而,这些差异在数量上很小。因此,将优质羔羊育肥至高水平的胴体总脂肪对任何与肌内脂肪比例增加相关的饮食质量益处几乎没有影响。
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引用次数: 43
期刊
Australian Journal of Experimental Agriculture
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