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Intramuscular fat levels in sheep muscle during growth 绵羊生长期间肌内脂肪水平
Pub Date : 2008-06-18 DOI: 10.1071/EA08046
M. McPhee, D. Hopkins, D. Pethick
A5 ·4 factorial experiment was designed in which lambs representing five genotypes were slaughtered at four ages (110, 236, 412 and 662 days of age). The genotypes represented were Poll Dorsetgrowth ·Border Leicester Merino, Poll Dorsetgrowth ·Merino, Poll Dorsetmuscling ·Merino, Merino ·Merino and Border Leicester ·Merino. Both sexes (ewes and wethers) were represented for each genotype and slaughter age combination. In total, 595 animals were slaughtered and the carcass composition and intramuscular fat were measured. Carcass composition (fat, ash and protein (lean)) was determined by dual energy X-ray absorptiometry, with the intramuscular fat percentage determined using near- infrared spectroscopy following removal and weighing of the entire longissimus thoracis et lumborum (LL) muscle. Analysis revealed that the proportion of intramusular fat in the loin relative to total carcass fat decreases as animals mature, thus indicating that intramusular fat deposition occurs early in the maturation of sheep. Furthermore, as animals became heavier and older the accretion rate of intramuscular fat in the LL muscle slowed down. Both genotype (P < 0.05) and sex (P < 0.001) were found to impact on this pattern, with Border Leicester ·Merino animals exhibiting the largest increase in intramuscular fat proportion in the LL muscle (4.92 and 5.50% at 22 months of age for ewes and wethers, respectively). The Poll Dorsetgrowth ·Border Leicester Merino animals were found to have the greatest absolute levels of intramuscular fat in the whole LL muscle (80.95 and 97.60g at maturity for ewes and wethers, respectively). The amount of intramuscular fat significantly increased as the sheep became older and fatter; however, these differences were quantitatively small. As such, finishing prime lambs to high levels of total carcass fatness would have little effect on any eating quality benefits associated with increased intramuscular fat proportion.
设计5·4因子试验,选取5种基因型的羔羊,分别在4个日龄(110、236、412和662日龄)屠宰。所代表的基因型为Poll多赛特生长·边境莱斯特美利奴、Poll多赛特生长·美利奴、Poll多赛特肌肉·美利奴、美利奴·美利奴和边境莱斯特·美利奴。每种基因型和屠宰年龄组合均代表了两性(母羊和公羊)。共屠宰595只动物,测定胴体成分和肌内脂肪含量。胴体组成(脂肪、灰分和蛋白质(瘦肉))采用双能x射线吸收仪测定,肌内脂肪百分比采用近红外光谱法测定,然后切除并称量整个胸腰最长肌(LL)。分析表明,随着动物的成熟,腰部肌内脂肪相对于胴体总脂肪的比例降低,这表明肌内脂肪沉积发生在绵羊成熟早期。此外,随着动物体重的增加和年龄的增长,左腰肌肌内脂肪的增加速度减慢。基因型(P < 0.05)和性别(P < 0.001)均对这一模式有影响,其中边境莱斯特·美利奴动物的LL肌肌内脂肪比例增加最多,母羊和公羊22月龄时分别增加4.92%和5.50%。结果表明,波尔多塞特生长·边境莱斯特美利奴羊的肌内脂肪绝对含量最高(母羊和公羊成熟时分别为80.95和97.60g)。随着绵羊年龄的增长和体重的增加,肌内脂肪含量显著增加;然而,这些差异在数量上很小。因此,将优质羔羊育肥至高水平的胴体总脂肪对任何与肌内脂肪比例增加相关的饮食质量益处几乎没有影响。
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引用次数: 43
Diet quality and liveweight gain of steers grazing Leucaena–grass pasture estimated with faecal near infrared reflectance spectroscopy (F.NIRS) 利用粪便近红外反射光谱(F.NIRS)评价长尾草-草地放牧阉牛的饲粮品质和活重
Pub Date : 2008-06-18 DOI: 10.1071/EA08007
R. Dixon, D. Coates
Three drafts of Bos indicus cross steers (initially 178-216 kg) grazed Leucaena-grass pasture [Leucaena leucocephala subspecies glabrata cv. Cunningham with green panic (Panicum maximum cv. trichoglume)] from late winter through to autumn during three consecutive years in the Burnett region of south-east Queensland. Measured daily weight gain (DWGActual) of the steers was generally 0.7-1.1 kg/day during the summer months. Estimated intakes of metabolisable energy and dry matter (DM) were calculated from feeding standards as the intakes required by the steers to grow at the DWGActual. Diet attributes were predicted from near infrared reflectance spectroscopy spectra of faeces (F.NIRS) using established calibration equations appropriate for northern Australian forages. Inclusion of some additional reference samples from cattle consuming Leucaena diets into F.NIRS calibrations based on grass and herbaceous legume-grass pastures improved prediction of the proportion of Leucaena in the diet. Mahalanobis distance values supported the hypothesis that the F.NIRS predictions of diet crude protein concentration and DM digestibility (DMD) were acceptable. F.NIRS indicated that the percentage of Leucaena in the diet varied widely (10-99%). Diet crude protein concentration and DMD were usually high, averaging 12.4 and 62%, respectively, and were related asymptotically to the percentage of Leucaena in the diet (R2 = 0.48 and 0.33, respectively). F.NIRS calibrations for DWG were not satisfactory to predict this variable from an individual faecal sample since the s.e. of prediction were 0.33-0.40 kg/day. Cumulative steer liveweight (LW) predicted from F.NIRS DWG calibrations, which had been previously developed with tropical grass and grass-herbaceous legume pastures, greatly overestimated the measured steer LW; therefore, these calibrations were not useful. Cumulative steer LW predicted from a modified F.NIRS DWG calibration, which included data from the present study, was strongly correlated (R2 = 0.95) with steer LW but overestimated LW by 19-31 kg after 8 months. Additional reference data are needed to develop robust F.NIRS calibrations to encompass the diversity of Leucaena pastures of northern Australia. In conclusion, the experiment demonstrated that F.NIRS could improve understanding of diet quality and nutrient intake of cattle grazing Leucaena-grass pasture, and the relationships between nutrient supply and cattle growth.
3批杂交籼牛(初始体重178 ~ 216公斤)放牧银合欢草甸[银合欢亚种glabrata cv.]。坎宁安有绿色恐慌(恐慌菌最大cv)。在昆士兰州东南部的伯内特地区,从冬末到秋天,连续三年都会出现这种植物。在夏季,阉牛的实测日增重(DWGActual)一般为0.7-1.1 kg/d。代谢能和干物质(DM)的估计采食量是根据饲粮标准计算的,作为在DWGActual生长所需的采食量。利用建立的适用于澳大利亚北部牧草的校准方程,从粪便的近红外反射光谱(F.NIRS)预测饲粮属性。将食用银合欢日粮的牛的一些额外参考样品纳入基于草和草本豆科草牧场的近红外光谱校准中,可以提高对日粮中银合欢比例的预测。马氏距离值支持F.NIRS预测日粮粗蛋白质浓度和DM消化率(DMD)的假设。近红外光谱分析表明,鱼青菜在饮食中的比例差异很大(10-99%)。饲粮粗蛋白质浓度和DMD均较高,平均分别为12.4%和62%,且与饲料中银合子的比例呈渐近相关(R2分别为0.48和0.33)。近红外光谱(F.NIRS)对DWG的校准不能令人满意地从单个粪便样本中预测这一变量,因为预测的正确率为0.33-0.40 kg/天。利用fnirs DWG校准预测的累积阉牛活重(LW),以前是用热带草和草-草本豆科牧草开发的,大大高估了实测的阉牛LW;因此,这些校准是没有用的。根据修正的F.NIRS DWG校准(包括本研究的数据)预测的累积转向LW与转向LW呈强相关(R2 = 0.95),但8个月后LW高估了19-31 kg。需要更多的参考数据来开发可靠的近红外光谱校准,以涵盖澳大利亚北部Leucaena牧场的多样性。综上所述,利用近红外光谱可以更好地了解采食白毛草草地的牛的日粮质量和营养摄入量,以及营养供应与牛生长的关系。
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引用次数: 15
Putting Merino weaner management recommendations to the test 将美利奴断奶仔猪管理建议付诸实践
Pub Date : 2008-06-18 DOI: 10.1071/EA08003
J. E. Edwards, R. Gould, K. J. Copping
Merino weaner survival is poor under normal traditional management practices. Data from four trials conducted between 2001 and 2005 were used to assess the effectiveness of current industry recommendations to achieve 95% survival rates of Merino weaners. There was very little difference between 2001 and 2003 in survival of Merino weaners despite average weaning weight ranging from 20 to 24 kg between years. The recommended target weaning weight of 23 kg does not always appear to be a good predictor of survival. However, a target of 45% mature liveweight at pasture senescence achieved the industry recommendation of 95% weaner survival. Merino weaners were fed over the summer–autumn period to reach 40 kg liveweight by the time green feed became available. Weaner survival reached 93% compared with 89% survival to hogget shearing in weaners managed under normal growth paths. Furthermore, twice as many ewe weaners died in the traditional feeding program compared with the well fed ewe weaners. Differences in mortality between feeding levels in the wether weaners was less than that observed in the ewe weaners. Flock average growth rates were not useful indicators of weaner survival and there was no relationship between Merino ram estimated breeding values for weaning weight and the survival of the progeny.
在传统的管理方法下,美利奴断奶仔猪的存活率很低。2001年至2005年期间进行的四项试验的数据用于评估当前行业建议实现美利奴断奶仔猪95%存活率的有效性。2001年和2003年期间,尽管平均断奶体重在20 - 24公斤之间,但美利奴断奶仔猪的存活率差异很小。建议的目标断奶体重23公斤似乎并不总是一个很好的预测生存。然而,在牧草衰老期,45%的成熟活重目标达到了95%断奶成活率的行业推荐值。夏秋两季饲喂美利奴断奶仔猪,到绿饲料可用时达到40公斤活重。正常生长路径下断奶仔猪的成活率为89%,而正常生长路径下断奶仔猪的成活率为93%。此外,在传统喂养方案中,断奶母羊的死亡率是喂养良好的断奶母羊的两倍。在饲喂水平之间,断奶仔猪死亡率的差异小于母羊断奶仔猪。羊群平均生长率不是断奶成活率的有效指标,美利奴公羊断奶体重的估计育种值与后代成活率之间没有关系。
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引用次数: 12
Faecal near infrared reflectance spectroscopy estimates of diet quality and responses to nitrogen supplements by cattle grazing Bothriochloa pertusa pastures 粪近红外反射光谱估计放牧牛日粮质量和对氮补充的响应
Pub Date : 2008-06-18 DOI: 10.1071/EA08004
D. Coates, R. Dixon
A grazing experiment in the seasonally dry tropics of north Queensland examined the diet selected and the growth responses of Bos indicus steers to urea supplement over two dry seasons and one wet season, from August 2001 to January 2003. There were two groups of 10 steers (control and urea-supplemented) and each group comprised two age cohorts, A and B, of five steers each with an age difference of ~1 year. In June 2002, cohort A steers were replaced with steers 2 years younger (cohort C). The steers grazed Bothriochloa pertusa pastures on a low fertility Red Chromosol soil. The groups were switched between two adjoining paddocks at fortnightly intervals when they were weighed and faecal samples were collected for faecal near infrared reflectance spectroscopy (F.NIRS) estimates of diet quality and growth rate. Rainfall and diet quality followed the expected seasonal pattern, but the 2001–02 wet season was very short (November–January) with only 65% of the long-term average rainfall. There was no rain during the 2001 dry season (DS-1) from August to mid November, and no effective rain (17 mm) between February and December 2002. Non-grass (i.e. herbaceous dicot plants) made only a small contribution to the diet, averaging 13%. In DS-1 and in the dry season of 2002 (DS-2) diet crude protein (CP) averaged 2.5% and 2.9%, and DMD/CP (ratio of dry matter digestibility to CP) averaged 18.6 and 17.1, respectively. Liveweight (LW) loss in control steers during DS-1 averaged 32 kg. Urea supplement reduced LW loss by 18 kg (P < 0.001), but most of the benefit was lost during the following growing season. During the extended dry period in 2002, average LW losses of control steers were 85 and 47 kg in the older and younger cohorts, respectively, and supplementation with urea reduced weight losses by 53 and 31 kg, respectively (P < 0.001). F.NIRS predicted the cumulative LW of the unsupplemented steers in cohorts A and B with acceptable accuracy, the difference between the observed and predicted final LW being 6 kg for cohort A after 10 months, 12 kg for cohort B after 17 months, and 27 kg for cohort C after 7 months. The results demonstrated that F.NIRS can be effectively used to monitor dietary CP and DMD levels in grazing cattle, to help develop an understanding of cattle responses to urea supplement relative to the quality of the basal forage diet, and to provide useful decision support information for the nutritional management of grazing cattle.
2001年8月至2003年1月,在昆士兰州北部季节性干燥热带地区进行了一项放牧试验,研究了两个旱季和一个雨季籼稻牛的日粮选择和对尿素补充的生长反应。试验分为两组,每组10头(对照组和加尿素组),每组分为A组和B组,每组5头,年龄相差1岁左右。2002年6月,A组阉牛被2岁以下的阉牛(C组)所取代。这些阉牛在低肥力的红色土壤上放牧波斯洛马牧场。每隔两周将各组轮换到两个相邻的围场,称重时收集粪便样本,用于粪便近红外反射光谱(F.NIRS)估计饮食质量和生长速度。降水和饲料质量符合预期的季节模式,但2001-02年雨季非常短(11月至1月),仅为长期平均降雨量的65%。2001年旱季(DS-1) 8月至11月中旬无雨,2002年2月至12月无有效雨量(17毫米)。非草类植物(即草本菊科植物)对饮食的贡献很小,平均为13%。DS-1和2002年旱季(DS-2)日粮粗蛋白质(CP)平均为2.5%和2.9%,干物质消化率/CP (DMD/CP)平均为18.6和17.1。在DS-1期间,对照肉牛的活重损失平均为32公斤。添加尿素可减少18 kg的低重损失(P < 0.001),但大部分效益在接下来的生长季节消失。在2002年延长的干旱期,老龄组和年轻组对照阉牛的平均体重损失分别为85和47公斤,补充尿素分别减少了53和31公斤的体重损失(P < 0.001)。fnirs预测A组和B组未补充的公牛的累积LW具有可接受的准确性,观察到的和预测的最终LW之间的差异在10个月后A组为6 kg,在17个月后B组为12 kg,在7个月后C组为27 kg。结果表明,近红外光谱可有效监测放牧牛饲粮CP和DMD水平,有助于了解牛对尿素补充的反应与基础饲粮质量的关系,并为放牧牛的营养管理提供有用的决策支持信息。
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引用次数: 13
Development and integration of animal welfare standards into company quality assurance programs in the Australian livestock (meat) processing industry 在澳大利亚家畜(肉类)加工业中,将动物福利标准发展和整合到公司质量保证计划中
Pub Date : 2008-06-18 DOI: 10.1071/EA08024
M. K. Edge, J. L. Barnett
A collaborative project between the Australian Meat Industry Council and the Animal Welfare Science Centre, a Centre partnered by the University of Melbourne, Department of Primary Industries (Victoria) and Monash University, that commenced in 2005, resulted in the development of a series of industry animal welfare standards designed to complement existing regulatory and commercial requirements. The project was the fourth in a series of similar projects conducted since 1998 for the poultry, pork and dairy industries, that was aimed at developing industry-wide animal welfare audit material. This concept was further developed to result in national industry animal welfare standards with accompanying audit material that could be integrated with existing industry, commercial and regulatory quality assurance documentation to demonstrate animal welfare practices. The resultant animal welfare standards were accompanied by additional implementation and reference information to enable integration and uptake within the industries and for the delivery of assurance on animal welfare to Governments, customers, trading partners and the consumer.
澳大利亚肉类工业委员会和动物福利科学中心(墨尔本大学、维多利亚第一产业部和莫纳什大学的合作中心)于2005年启动了一个合作项目,旨在制定一系列行业动物福利标准,以补充现有的监管和商业要求。该项目是自1998年以来为家禽、猪肉和乳制品行业开展的一系列类似项目中的第四个,旨在开发全行业动物福利审计材料。这一概念得到进一步发展,形成了国家工业动物福利标准,并附带审计材料,这些材料可以与现有的工业、商业和监管质量保证文件相结合,以证明动物福利实践。由此产生的动物福利标准还附有额外的执行和参考资料,以便在工业内部进行整合和吸收,并向政府、顾客、贸易伙伴和消费者提供动物福利保证。
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引用次数: 6
DairyMod and EcoMod: biophysical pasture-simulation models for Australia and New Zealand DairyMod和EcoMod:澳大利亚和新西兰的生物物理牧场模拟模型
Pub Date : 2008-04-28 DOI: 10.1071/EA07133
I. R. Johnson, D. Chapman, V. Snow, R. Eckard, A. Parsons, M. G. Lambert, B. Cullen
DairyMod and EcoMod, which are biophysical pasture-simulation models for Australian and New Zealand grazing systems, are described. Each model has a common underlying biophysical structure, with the main differences being in their available management options. The third model in this group is the SGS Pasture Model, which has been previously described, and these models are referred to collectively as ‘the model’. The model includes modules for pasture growth and utilisation by grazing animals, water and nutrient dynamics, animal physiology and production and a range of options for pasture management, irrigation and fertiliser application. Up to 100 independent paddocks can be defined to represent spatial variation within a notional farm. Paddocks can have different soil types, nutrient status, pasture species, fertiliser and irrigation management, but are subject to the same weather. Management options include commonly used rotational grazing management strategies and continuous grazing with fixed or variable stock numbers. A cutting regime simulates calculation of seasonal pasture growth rates. The focus of the present paper is on recent developments to the management routines and nutrient dynamics, including organic matter, inorganic nutrients, leaching and gaseous nitrogen losses, and greenhouse gases. Some model applications are presented and the role of the model in research projects is discussed.
描述了澳大利亚和新西兰放牧系统的生物物理牧场模拟模型DairyMod和EcoMod。每个模型都有一个共同的基础生物物理结构,主要区别在于它们可用的管理选项。这组中的第三个模型是SGS牧场模型,前面已经描述过了,这些模型统称为“模型”。该模型包括放牧动物的牧草生长和利用、水和养分动态、动物生理和生产以及牧场管理、灌溉和施肥的一系列选择。可以定义多达100个独立的围场来表示一个概念农场内的空间变化。围场可以有不同的土壤类型、养分状况、牧草种类、肥料和灌溉管理,但受制于相同的天气。管理方案包括常用的轮牧管理策略和固定或可变牲畜数量的连续放牧。割草制度模拟了牧草季节性生长率的计算。本文的重点是最近的发展,管理程序和营养动态,包括有机物质,无机养分,淋滤和气态氮损失,以及温室气体。介绍了模型的一些应用,并讨论了模型在研究项目中的作用。
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引用次数: 201
Water management as a future necessity in sheep feedlots 水管理是绵羊饲养场未来的必需品
Pub Date : 2008-04-28 DOI: 10.1071/EA06282
J. Pluske, A. Schlink
Improving water management in rural towns such as Wagin, Western Australia, will decrease infrastructure damage caused by water and salinity and produce a ‘new water’ resource. The aim of this paper is to predict feedlot water demand using a bioeconomic model, H20Sheep, to determine if using such a ‘new water’ resource could be a viable option for this production system. Wagin (–33.3075 S, 117.3403 E), a township south-east of Perth, was chosen as the specific location for a sheep feedlot producing prime lambs. In this paper, the H20Sheep model was used to show how feedlot returns are influenced by the price of water, different feeding regimens and climate change. This was done by integrating feed and water intake of lambs, general feedlot water use and waste disposal. To show relative sensitivity of changing other model parameters that are not directly connected with water, changes in the purchase and sale price of lambs were also investigated. As might be expected, H20Sheep shows that returns from a sheep feedlot enterprise can be extremely sensitive to changes in lamb purchase (just over 7% increase will result in negative returns) and sale prices (a 4% decrease will generate a negative outcome). With respect to water, the findings indicate that, while increases in water use in the feedlot and price have to be greater than the increase in relative price of sheep, monitoring the biological parameters associated with water as well as water prices is still important both from a management and an economic perspective. Hence, if towns involved in the Rural Towns – Liquid Assets project, such as Wagin, decide to sell their water, the relevant policy makers should ensure that the sale price enables an effective water management system for the town and is also attractive to end-users such as feedlots.
改善西澳大利亚州瓦金等农村城镇的水资源管理,将减少水和盐分对基础设施造成的破坏,并产生一种“新的水资源”。本文的目的是使用生物经济模型H20Sheep来预测饲养场的水需求,以确定使用这种“新水”资源是否可能是该生产系统的可行选择。瓦金(-33.3075 S, 117.3403 E)是珀斯东南部的一个乡镇,被选为生产优质羔羊的绵羊饲养场的具体地点。本文采用H20Sheep模型分析了水价、不同饲养方式和气候变化对饲养场收益的影响。这是通过综合羔羊的饲料和水摄入量,一般饲养场用水和废物处理来完成的。为了显示其他与水没有直接关系的模型参数变化的相对敏感性,还研究了羔羊购销价格的变化。正如预期的那样,H20Sheep表明,羊饲养场企业的回报对羔羊购买量(仅仅增加7%以上就会导致负回报)和销售价格(减少4%就会产生负结果)的变化非常敏感。关于水,研究结果表明,虽然饲养场用水和价格的增长必须大于羊的相对价格的增长,但从管理和经济角度来看,监测与水有关的生物参数以及水价仍然很重要。因此,如果参与农村城镇-流动资产项目的城镇,如瓦金,决定出售其水,相关政策制定者应确保销售价格使城镇能够建立有效的水管理系统,并对饲养场等最终用户具有吸引力。
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引用次数: 5
Influence of biotin supplementation on hoof growth and integrity in long-fed F1 Wagyu/Black Angus feedlot steers of known genotype 添加生物素对已知基因型F1和/黑安格斯长期饲养阉牛蹄生长和完整性的影响
Pub Date : 2008-04-28 DOI: 10.1071/EA07010
R. J. Lawrence, R. Elliott, B. Norton, M. B. Thoefner, I. Loxton, G. Webber
Biotin was supplemented at three levels (0, 10 and 20 mg/steer.day) to 108 F1 Wagyu/Black Angus steers of known genotype, which were fed a wheat-based ration, to evaluate the effect on hoof growth, wear and integrity within feedlot pens. One hundred and eight steers of known sire lines were assigned to three biotin treatments (0, 10 and 20 mg/steer.day) with each treatment replicated four times using an unfasted liveweight of 410.5kg (s.e. ± 2.27, s.d. ± 24.41). A subset of 36 steers was randomly selected for monitoring of hoof growth on seven occasions, over a period of 301 days. Front hooves from selected steers within turn-off group were collected at the abattoir and investigated for hoof integrity through claw lesions, white line width and dry matter of both white line and hoof. Hoof growth rates over time remained relatively consistent. In contrast, hoof wear rates increased (P 0.05) hoof growth or wear. Examination of collected hooves revealed lesions present on both lateral and medial claws. Biotin supplementation had no effect (P > 0.05) on lesion number, white line width of the lateral and medial claws, or hoof and white line dry matter of the medial claw. In contrast, Grandsire had an effect on total hoof length at measurement periods 0, 167, 251 and 301 (P < 0.05), and also on white line width of both lateral (P = 0.015) and medial (P = 0.002) claws. Rainfall and related increase in pen moisture weakened hoof integrity by increasing hoof moisture content and hoof wear. Genotype appears to play a role in hoof integrity.
在108头已知基因型的F1和牛/黑安格斯阉牛饲粮中添加3个水平(0、10和20 mg/头.d)的生物素,以小麦为基础,评价其对猪圈内蹄生长、磨损和完整性的影响。108头已知品系的阉牛被分配到3个生物素处理组(0、10和20 mg/头/天),每个处理重复4次,未空腹活重410.5kg (s.e±2.27,s.d±24.41)。在301天的时间里,随机选择36头阉牛进行7次蹄生长监测。在屠宰场收集关闭组中选定的牛的前蹄,通过爪损、白线宽度和白线和蹄的干物质来研究蹄的完整性。随着时间的推移,蹄的增长率保持相对稳定。相比之下,马蹄磨损率显著提高(p0.05)。检查收集的蹄发现病变存在于两侧和内侧的爪子。添加生物素对爪外侧和内侧的病变数、白线宽度、爪内侧的蹄和白线干物质无显著影响(P < 0.05)。相比之下,祖辈对第0、167、251和301测量期的总蹄长有影响(P < 0.05),对外侧(P = 0.015)和内侧(P = 0.002)爪白线宽度也有影响。降雨和相关的畜栏水分的增加通过增加蹄的水分含量和蹄的磨损削弱了蹄的完整性。基因型似乎在蹄的完整性中起作用。
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引用次数: 0
Effect of surface applied glycine betaine on herbage production and quality of perennial ryegrass–white clover pastures 面施甜菜碱对多年生黑麦草-白三叶草牧草产量和品质的影响
Pub Date : 2008-04-28 DOI: 10.1071/EA07123
Julia M. Lee, K. Elborough, W. Catto, D. Donaghy, J. Roche
Osmoprotectants have been reported to reduce the detrimental effects of various environmental stresses in many different plant species. However, there is little research available concerning pasture species. Two experiments were undertaken with the aim of quantifying the effect of surface applications of exogenous glycine betaine (GB) on herbage production and quality of perennial ryegrass (Lolium perenne L.)–white clover (Trifolium repens L.) pastures during periods of moisture stress and cold temperatures over 2 years. Pastures fertilised with GB were compared with unfertilised pastures and pastures fertilised with nitrogen (N). Rates of 0.5, 1.0 and 1.5 kg GB/ha.defoliation were applied in experiment 1 and 5 kg GB/ha.defoliation was applied in experiment 2. Surface applications of GB did not significantly affect herbage production relative to unfertilised pastures; herbage yields averaged 12 248 and 12 693 kg DM/ha over 11 months in experiment 1, and 7253 and 7177 kg DM/ha over 6 months during summer and autumn in experiment 2, for the unfertilised control and GB, respectively. During both experiments, herbage quality parameters were not affected by GB application, although the proportion of white clover in the sward between summer and winter during experiment 1 was greater (P < 0.01) in plots treated with GB than in untreated plots. Application of N fertiliser increased (P < 0.001) herbage production, but did not consistently affect herbage quality. The failure of surface applications of exogenous GB to improve the herbage production or quality of perennial ryegrass–white clover pastures suggests that it is not an appropriate method to enhance plant tolerance to environmental stress at the concentrations applied in these studies.
据报道,渗透保护剂可以减少各种环境胁迫对许多不同植物物种的有害影响。然而,关于牧草种类的研究却很少。在2年多的水分胁迫和低温条件下,研究了外源甜菜碱(GB)对多年生黑麦草-白三叶草牧草产量和品质的影响。试验1和试验2分别采用0.5、1.0和1.5 kg GB/ha和5 kg GB/ha的叶片剥落速率进行叶片剥落试验。相对于未施肥的牧场,地表施用GB对牧草产量影响不显著;试验1的11个月平均牧草产量为12 248和12 693 kg DM/ hm2,试验2的夏季和秋季平均产量为7253和7177 kg DM/ hm2。在两个试验中,施用GB对牧草品质参数均无影响,但试验1中施GB的地块夏冬季节草地中白三叶草的比例高于未施GB的地块(P < 0.01)。施氮量增加了牧草产量(P < 0.001),但对牧草品质影响不一致。表面施用外源GB不能提高多年生黑麦草-白三叶草草场的牧草产量或质量,这表明在本研究中施用的浓度下,提高植物对环境胁迫的耐受性并不是一种合适的方法。
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引用次数: 3
Notch pathway gene expression and wool follicle cell fates Notch通路基因表达与毛囊细胞命运的关系
Pub Date : 2008-04-28 DOI: 10.1071/EA07315
C. Gordon‐Thomson, S. Botto, G. Cam, G. Moore
The Notch family of genes has been implicated in specifying cell fates during hair follicle morphogenesis. We examined Notch gene expression during wool follicle formation, as an understanding of genes that influence cell distributions in the developing follicle is a prerequisite for devising molecular strategies to manipulate fibre characters and follicle density. We identified transcripts for the Notch1 receptor and one of its ligands, Jagged1, in fetal sheep skin by reverse transcriptase polymerase chain reaction. The sheep-specific cDNA sequences were used as templates to produce probes to investigate the expression patterns of Notch1 and Jagged1 in developing ovine fetal skin by in situ hybridisation. Notch1 and Jagged1 were detected in the epidermis and in a subpopulation of mesenchymal cells before follicle initiation. At day 70 during follicle initiation, transcripts were also detected in cells at the tip of the epidermal plug and in dermal condensates. By day 86, Notch1 and Jagged1 were detected in the distal cells of the epidermal downgrowths and epidermis and Notch1 was no longer detected in the mesenchyme and dermal condensates. After day 96, transcripts were absent from the epidermis, but localised to differentiating outer root sheath (ORS) cells. The distributions of transcripts implicate a Notch1-Jagged1 signal pathway in the fates of prospective ORS cells. The transient appearance of Notch1 in cells at the epidermal-mesenchymal junction during early follicle morphogenesis suggests that the receptor may be responsible for the specification of a cell subpopulation committed to a prepapilla fate at initiation.
Notch基因家族在毛囊形态发生过程中被认为与细胞命运有关。我们研究了Notch基因在毛囊形成过程中的表达,因为了解影响毛囊发育中细胞分布的基因是设计分子策略来操纵纤维特性和毛囊密度的先决条件。我们通过逆转录酶聚合酶链反应在胎羊皮肤中鉴定了Notch1受体及其配体之一Jagged1的转录本。以绵羊特异性cDNA序列为模板,制备探针,原位杂交研究Notch1和Jagged1在绵羊胎皮发育中的表达规律。Notch1和Jagged1在卵泡形成前的表皮和间充质细胞亚群中检测到。在卵泡形成的第70天,在表皮塞顶端的细胞和真皮凝聚物中也检测到转录本。到第86天,表皮下生长和表皮远端细胞中检测到Notch1和Jagged1,间质和真皮凝析液中不再检测到Notch1。96天后,表皮中没有转录本,但定位于分化的外根鞘(ORS)细胞。转录本的分布暗示了Notch1-Jagged1信号通路在未来ORS细胞的命运中。在早期卵泡形态发生过程中,Notch1在表皮-间充质交界处的细胞中短暂出现,这表明该受体可能负责一个细胞亚群的指定,这些细胞亚群在开始时致力于毛前细胞的命运。
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引用次数: 5
期刊
Australian Journal of Experimental Agriculture
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