首页 > 最新文献

Ernst Schering Foundation symposium proceedings最新文献

英文 中文
In vivo characterization of progestins with reduced non-genomic activity in vitro. 体外非基因组活性降低的孕激素的体内表征。
C Otto, B Rohde-Schulz, G Schwarz, I Fuchs, M Klewer, H Altmann, K H Fritzemeier

Postmenopausal women that still have an uterus and suffer from hot flushes are treated with combinations of estrogens and progestins. Whereas estrogens are indispensable for treating postmenopausal symptoms, progestins are added to counteract the proliferative activity of estrogens on uterine epithelial cells. However, in the mammary gland, progestins, given together with estrogens, stimulate the proliferation of mammary epithelial cells. Therefore, progestins with reduced proliferative activity in the mammary gland would be of advantage for hormone therapy of postmenopausal women. In order to identify progestins with better tissue-selectivity, we exploited the activation of different signal transduction pathways by the classical progesterone receptor. We demonstrated that progestins with reduced non-genomic versus genomic activity in vitro show a better dissociation of uterine versus mammary gland effects in vivo than medroxyprogesterone acetate (MPA), a synthetic progestin that is widely used in hormone therapy.

绝经后仍有子宫并患有潮热的妇女可以用雌激素和黄体酮联合治疗。虽然雌激素对于治疗绝经后症状是必不可少的,但添加孕激素是为了抵消雌激素对子宫上皮细胞的增殖活性。然而,在乳腺中,黄体酮与雌激素一起使用会刺激乳腺上皮细胞的增殖。因此,降低乳腺增殖活性的黄体酮将有利于绝经后妇女的激素治疗。为了鉴定具有更好组织选择性的黄体酮,我们利用了经典黄体酮受体激活的不同信号转导途径。我们证明体外非基因组活性和基因组活性降低的孕激素在体内比醋酸甲羟孕酮(MPA)表现出更好的子宫和乳腺分离作用,醋酸甲羟孕酮是一种广泛用于激素治疗的合成孕激素。
{"title":"In vivo characterization of progestins with reduced non-genomic activity in vitro.","authors":"C Otto,&nbsp;B Rohde-Schulz,&nbsp;G Schwarz,&nbsp;I Fuchs,&nbsp;M Klewer,&nbsp;H Altmann,&nbsp;K H Fritzemeier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Postmenopausal women that still have an uterus and suffer from hot flushes are treated with combinations of estrogens and progestins. Whereas estrogens are indispensable for treating postmenopausal symptoms, progestins are added to counteract the proliferative activity of estrogens on uterine epithelial cells. However, in the mammary gland, progestins, given together with estrogens, stimulate the proliferation of mammary epithelial cells. Therefore, progestins with reduced proliferative activity in the mammary gland would be of advantage for hormone therapy of postmenopausal women. In order to identify progestins with better tissue-selectivity, we exploited the activation of different signal transduction pathways by the classical progesterone receptor. We demonstrated that progestins with reduced non-genomic versus genomic activity in vitro show a better dissociation of uterine versus mammary gland effects in vivo than medroxyprogesterone acetate (MPA), a synthetic progestin that is widely used in hormone therapy.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 1","pages":"151-70"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27487551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pyruvate kinase type M2: a key regulator within the tumour metabolome and a tool for metabolic profiling of tumours. 丙酮酸激酶M2型:肿瘤代谢组的关键调节因子和肿瘤代谢谱的工具。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_091
S Mazurek

Normal proliferating cells and tumour cells in particular express the pyruvate kinase isoenzyme type M2 (M2-PK, PKM2). The quaternary structure of M2-PK determines whether the glucose carbons are degraded to pyruvate and lactate with production of energy (tetrameric form) or channelled into synthetic processes, debranching from glycolytic intermediates such as nucleic acid, amino acid and phospholipid synthesis. The tetramer:dimer ratio of M2-PK is regulated by metabolic intermediates, such as fructose 1,6-P2 and direct interaction with different oncoproteins, such as pp60v-src kinase, HPV-16 E7 and A-Raf. The metabolic function of the interaction between M2-PK and the HERC1 oncoprotein remains unknown. Thus, M2-PK is a meeting point for different oncogenes and metabolism. In tumour cells, the dimeric form of M2-PK is predominant and has therefore been termed Tumour M2-PK. Tumour M2-PK is released from tumours into the blood and from gastrointestinal tumours also into the stool of tumour patients. The quantification of Tumour M2-PK in EDTA plasma and stool is a tool for early detection of tumours and therapy control.

正常增殖细胞和肿瘤细胞表达丙酮酸激酶同工酶M2 (M2- pk, PKM2)。M2-PK的季元结构决定了葡萄糖碳是通过产生能量(四聚体形式)降解为丙酮酸盐和乳酸盐,还是通过糖酵解中间体(如核酸、氨基酸和磷脂合成)的脱支进入合成过程。M2-PK的四聚体:二聚体比例受代谢中间体(如果糖1、6-P2)和与不同癌蛋白(如pp60v-src激酶、HPV-16 E7和A-Raf)的直接相互作用调节。M2-PK与HERC1癌蛋白相互作用的代谢功能尚不清楚。因此,M2-PK是不同癌基因和代谢的交汇点。在肿瘤细胞中,二聚体形式的M2-PK占主导地位,因此被称为肿瘤M2-PK。肿瘤M2-PK从肿瘤释放到血液中,也从胃肠道肿瘤释放到肿瘤患者的粪便中。EDTA血浆和粪便中肿瘤M2-PK的定量是肿瘤早期检测和治疗控制的工具。
{"title":"Pyruvate kinase type M2: a key regulator within the tumour metabolome and a tool for metabolic profiling of tumours.","authors":"S Mazurek","doi":"10.1007/2789_2008_091","DOIUrl":"https://doi.org/10.1007/2789_2008_091","url":null,"abstract":"<p><p>Normal proliferating cells and tumour cells in particular express the pyruvate kinase isoenzyme type M2 (M2-PK, PKM2). The quaternary structure of M2-PK determines whether the glucose carbons are degraded to pyruvate and lactate with production of energy (tetrameric form) or channelled into synthetic processes, debranching from glycolytic intermediates such as nucleic acid, amino acid and phospholipid synthesis. The tetramer:dimer ratio of M2-PK is regulated by metabolic intermediates, such as fructose 1,6-P2 and direct interaction with different oncoproteins, such as pp60v-src kinase, HPV-16 E7 and A-Raf. The metabolic function of the interaction between M2-PK and the HERC1 oncoprotein remains unknown. Thus, M2-PK is a meeting point for different oncogenes and metabolism. In tumour cells, the dimeric form of M2-PK is predominant and has therefore been termed Tumour M2-PK. Tumour M2-PK is released from tumours into the blood and from gastrointestinal tumours also into the stool of tumour patients. The quantification of Tumour M2-PK in EDTA plasma and stool is a tool for early detection of tumours and therapy control.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 4","pages":"99-124"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/2789_2008_091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27690210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 79
Biomimetic organocatalytic C-C-bond formations. 仿生有机催化c - c键形成。
D Enders, M R M Hüttl, O Niemeier

Mother Nature utilizes simple precursors to build up complex organic molecules efficiently. One important example is the C3 building block dihydroxyacetone phosphate, which is used in various enzyme-catalyzed reactions. Following this biosynthetic strategy the DHAP equivalent 'dioxanone' can be used in organocatalytic reactions to synthesize sugars, aminosugars, carbasugars, polyoxamic acids and sphingosines. In this respect, organocatalytic domino reactions can also be seen as biomimetic as they are reminiscent of tandem reactions that may occur during biosyntheses of complex natural products. In nature, the coenzyme thiamin (vitamin B1), a natural thiazolium salt, is used in biochemical nucleophilic acylations ('Umpolung'). The catalytic active species is a nucleophilic carbene. Mimicking this approach, organocatalytic carbene catalysis has emerged to an exceptionally fruitful research area, which is used in asymmetric C-C bond formations.

大自然母亲利用简单的前体有效地构建复杂的有机分子。一个重要的例子是C3构建块磷酸二羟丙酮,它用于各种酶催化反应。按照这种生物合成策略,DHAP等效的“二恶酮”可用于有机催化反应,以合成糖、氨基糖、碳糖、聚肟酸和鞘氨醇。在这方面,有机催化多米诺反应也可以被看作是仿生的,因为它们让人想起在复杂天然产物的生物合成过程中可能发生的串联反应。在自然界中,辅酶硫胺素(维生素B1)是一种天然的噻唑盐,用于生化亲核酰化(Umpolung)。催化活性物质为亲核碳。模仿这种方法,有机催化二氧化碳催化已经出现了一个非常富有成果的研究领域,它用于不对称C-C键的形成。
{"title":"Biomimetic organocatalytic C-C-bond formations.","authors":"D Enders,&nbsp;M R M Hüttl,&nbsp;O Niemeier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Mother Nature utilizes simple precursors to build up complex organic molecules efficiently. One important example is the C3 building block dihydroxyacetone phosphate, which is used in various enzyme-catalyzed reactions. Following this biosynthetic strategy the DHAP equivalent 'dioxanone' can be used in organocatalytic reactions to synthesize sugars, aminosugars, carbasugars, polyoxamic acids and sphingosines. In this respect, organocatalytic domino reactions can also be seen as biomimetic as they are reminiscent of tandem reactions that may occur during biosyntheses of complex natural products. In nature, the coenzyme thiamin (vitamin B1), a natural thiazolium salt, is used in biochemical nucleophilic acylations ('Umpolung'). The catalytic active species is a nucleophilic carbene. Mimicking this approach, organocatalytic carbene catalysis has emerged to an exceptionally fruitful research area, which is used in asymmetric C-C bond formations.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 2","pages":"45-124"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27548210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biomimetic organocatalytic C-C-bond formations. 仿生有机催化c - c键形成。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2007_069
D. Enders, Matthias R. M. Hüttl, O. Niemeier
{"title":"Biomimetic organocatalytic C-C-bond formations.","authors":"D. Enders, Matthias R. M. Hüttl, O. Niemeier","doi":"10.1007/2789_2007_069","DOIUrl":"https://doi.org/10.1007/2789_2007_069","url":null,"abstract":"","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":"44 1","pages":"45-124"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73761137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Chiral organocatalysts for enantioselective photochemical reactions. 对映选择性光化学反应的手性有机催化剂。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2007_065
S. Breitenlechner, Philipp S. Selig, T. Bach
{"title":"Chiral organocatalysts for enantioselective photochemical reactions.","authors":"S. Breitenlechner, Philipp S. Selig, T. Bach","doi":"10.1007/2789_2007_065","DOIUrl":"https://doi.org/10.1007/2789_2007_065","url":null,"abstract":"","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":"27 1","pages":"255-79"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81203410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Molecular mechanisms that control leukocyte extravasation through endothelial cell contacts. 通过内皮细胞接触控制白细胞外渗的分子机制。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2007_063
D Vestweber

Leukocyte extravasation and entry into tissue forms the basis for inflammatory reactions and lymphocyte surveillance. After docking at the blood vessel wall at sites of exit leukocytes migrate through the endothelial cell layer and the underlying basement membrane, a process described as diapedesis. In recent years, several endothelial membrane proteins that which participate in this process have been identified. This review focuses on three membrane proteins located at endothelial cell contacts that are involved in the regulation of leukocyte diapedesis. The endothelial cell selective adhesion molecule (ESAM) at endothelial tight junctions and the vascular endothelial receptor-type protein tyrosine phosphatase (VE-PTP), a protein associating with VE-cadherin, both seem to control the integrity of endothelial cell contacts during diapedesis. CD99 and the distantly related CD99L2 are leukocyte membrane proteins that do not belong to any known protein family. They are expressed at endothelial cell contacts and participate in the migration of leukocytes through endothelium and basement membrane.

白细胞外渗和进入组织是炎症反应和淋巴细胞监视的基础。在血管壁出口处停靠后,白细胞通过内皮细胞层和其下的基底膜迁移,这一过程被称为渗出。近年来,已经发现了参与这一过程的几种内皮膜蛋白。本文综述了三种位于内皮细胞接触处的膜蛋白,它们参与了白细胞浸润的调节。内皮细胞紧密连接处的内皮细胞选择性粘附分子(ESAM)和血管内皮受体型蛋白酪氨酸磷酸酶(VE-PTP),一种与ve -钙粘蛋白相关的蛋白,似乎都控制着浸润过程中内皮细胞接触的完整性。CD99和远亲CD99L2是白细胞膜蛋白,不属于任何已知的蛋白家族。它们在内皮细胞接触处表达,参与白细胞通过内皮和基底膜的迁移。
{"title":"Molecular mechanisms that control leukocyte extravasation through endothelial cell contacts.","authors":"D Vestweber","doi":"10.1007/2789_2007_063","DOIUrl":"https://doi.org/10.1007/2789_2007_063","url":null,"abstract":"<p><p>Leukocyte extravasation and entry into tissue forms the basis for inflammatory reactions and lymphocyte surveillance. After docking at the blood vessel wall at sites of exit leukocytes migrate through the endothelial cell layer and the underlying basement membrane, a process described as diapedesis. In recent years, several endothelial membrane proteins that which participate in this process have been identified. This review focuses on three membrane proteins located at endothelial cell contacts that are involved in the regulation of leukocyte diapedesis. The endothelial cell selective adhesion molecule (ESAM) at endothelial tight junctions and the vascular endothelial receptor-type protein tyrosine phosphatase (VE-PTP), a protein associating with VE-cadherin, both seem to control the integrity of endothelial cell contacts during diapedesis. CD99 and the distantly related CD99L2 are leukocyte membrane proteins that do not belong to any known protein family. They are expressed at endothelial cell contacts and participate in the migration of leukocytes through endothelium and basement membrane.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 3","pages":"151-67"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/2789_2007_063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27469095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Dynamic regulation of progesterone receptor activity in female reproductive tissues. 雌性生殖组织中孕酮受体活性的动态调控。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2007_056
S J Han, F J DeMayo, B W O'Malley

The progesterone receptor (PR) in cooperation with coregulator complexes coordinates crucial processes in female reproduction. To investigate the dynamic regulation of PR activity in vivo, a new transgenic mouse model utilizing a PR activity indicator (PRAI) system was generated. Studies utilizing the PRAI mouse have revealed that progesterone temporally regulates PR activity in female reproductive tissues. Specifically, progesterone rapidly enhances PR activity immediately after administration. However, chronic progesterone stimulation represses PR activity in female reproductive organs. Like progesterone, RU486 also temporally regulates PR activity in female reproductive organs. However, the temporal regulation of PR activity by RU486 is the inverse of progesterone's activity. RU486 acutely represses PR activity after injection but increases PR activity after chronic treatment in female reproductive tissues. Treatment with a mixed antagonist/agonist of PR, when compared to natural hormone, results in dramatically different tissue-specific patterns of intracellular PR activity, coregulator levels, and kinase activity. Transcriptional regulation of gene expression by PR is facilitated by coordinate interactions with the steroid receptor coactivators (SRCs). Bigenic PRAI-SRC knockout mouse models enabled us to draw a tissue-specific coactivator atlas for PR activity in vivo. Based on this atlas, we conclude that the endogenous physiological function of PR in distinct tissues is modulated by different SRCs. SRC-3 is the primary coactivator for PR in the breast and SRC-1 is the primary coactivator for PR in the uterus.

孕激素受体(PR)与协同调节复合物合作协调女性生殖的关键过程。为了研究PR活性在体内的动态调控,利用PR活性指示剂(PRAI)系统构建了一种新的转基因小鼠模型。利用PRAI小鼠的研究表明,黄体酮可以暂时调节雌性生殖组织中的PR活性。具体来说,黄体酮在给药后立即迅速增强PR活动。然而,慢性黄体酮刺激会抑制女性生殖器官的PR活性。与黄体酮一样,RU486也能暂时调节女性生殖器官中的PR活性。然而,RU486对PR活性的时间调节与黄体酮的活性相反。在雌性生殖组织中,RU486注射后急性抑制PR活性,慢性治疗后PR活性升高。与天然激素相比,使用PR的混合拮抗剂/激动剂治疗会导致细胞内PR活性、共调节因子水平和激酶活性的组织特异性模式显著不同。PR与类固醇受体共激活因子(src)的协调相互作用促进了基因表达的转录调控。双基因prap - src敲除小鼠模型使我们能够绘制组织特异性PR活性的共激活因子图谱。基于这一图谱,我们得出结论,PR在不同组织中的内源性生理功能受到不同src的调节。SRC-3是乳腺PR的主要共激活因子,SRC-1是子宫PR的主要共激活因子。
{"title":"Dynamic regulation of progesterone receptor activity in female reproductive tissues.","authors":"S J Han,&nbsp;F J DeMayo,&nbsp;B W O'Malley","doi":"10.1007/2789_2007_056","DOIUrl":"https://doi.org/10.1007/2789_2007_056","url":null,"abstract":"<p><p>The progesterone receptor (PR) in cooperation with coregulator complexes coordinates crucial processes in female reproduction. To investigate the dynamic regulation of PR activity in vivo, a new transgenic mouse model utilizing a PR activity indicator (PRAI) system was generated. Studies utilizing the PRAI mouse have revealed that progesterone temporally regulates PR activity in female reproductive tissues. Specifically, progesterone rapidly enhances PR activity immediately after administration. However, chronic progesterone stimulation represses PR activity in female reproductive organs. Like progesterone, RU486 also temporally regulates PR activity in female reproductive organs. However, the temporal regulation of PR activity by RU486 is the inverse of progesterone's activity. RU486 acutely represses PR activity after injection but increases PR activity after chronic treatment in female reproductive tissues. Treatment with a mixed antagonist/agonist of PR, when compared to natural hormone, results in dramatically different tissue-specific patterns of intracellular PR activity, coregulator levels, and kinase activity. Transcriptional regulation of gene expression by PR is facilitated by coordinate interactions with the steroid receptor coactivators (SRCs). Bigenic PRAI-SRC knockout mouse models enabled us to draw a tissue-specific coactivator atlas for PR activity in vivo. Based on this atlas, we conclude that the endogenous physiological function of PR in distinct tissues is modulated by different SRCs. SRC-3 is the primary coactivator for PR in the breast and SRC-1 is the primary coactivator for PR in the uterus.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 1","pages":"25-43"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/2789_2007_056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27490409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Mammary development, carcinomas and progesterone: role of Wnt signalling. 乳腺发育、癌症和黄体酮:Wnt信号的作用。
R Lamb, H Harrison, R B Clarke

The mammary gland begins development during embryogenesis but after exposure to hormonal changes during puberty and pregnancy undergoes extensive further development. Hormonal changes are key regulators in the cycles of proliferation, differentiation, apoptosis and remodelling associated with pregnancy, lactation and involution following weaning. These developmental processes within the breast epithelium can be explained by the presence of a long-lived population of tissue-specific stem cells. The longevity of these stem cells makes them susceptible to accumulating genetic change and consequent transformation. The ovarian steroid progesterone, acting via the secreted factor Wnt4, is known to be essential for side branching of the mammary gland. One function of Wnt proteins is self-renewal of adult tissue stem cells, suggesting that progesterone may exert its effects within the breast, at least partly, by regulating the mammary stem cell population.

乳腺在胚胎发生期间开始发育,但在青春期和怀孕期间暴露于激素变化后,经历了广泛的进一步发育。激素变化是与妊娠、哺乳和断奶后复归相关的增殖、分化、凋亡和重塑周期的关键调节因子。乳腺上皮内的这些发育过程可以通过存在一个长寿的组织特异性干细胞群来解释。这些干细胞的寿命使它们易于积累遗传变化和随后的转化。卵巢类固醇黄体酮通过分泌因子Wnt4起作用,已知对乳腺侧分支至关重要。Wnt蛋白的一个功能是成体组织干细胞的自我更新,这表明黄体酮可能在乳房内发挥作用,至少部分是通过调节乳腺干细胞群来发挥作用的。
{"title":"Mammary development, carcinomas and progesterone: role of Wnt signalling.","authors":"R Lamb,&nbsp;H Harrison,&nbsp;R B Clarke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mammary gland begins development during embryogenesis but after exposure to hormonal changes during puberty and pregnancy undergoes extensive further development. Hormonal changes are key regulators in the cycles of proliferation, differentiation, apoptosis and remodelling associated with pregnancy, lactation and involution following weaning. These developmental processes within the breast epithelium can be explained by the presence of a long-lived population of tissue-specific stem cells. The longevity of these stem cells makes them susceptible to accumulating genetic change and consequent transformation. The ovarian steroid progesterone, acting via the secreted factor Wnt4, is known to be essential for side branching of the mammary gland. One function of Wnt proteins is self-renewal of adult tissue stem cells, suggesting that progesterone may exert its effects within the breast, at least partly, by regulating the mammary stem cell population.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 1","pages":"1-23"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27490503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New concepts for organocatalysis. 有机催化的新概念。
S C Pan, B List

Organocatalysis, catalysis with low-molecular weight catalysts in which a metal is not part of the catalytic principle or the reaction substrate, can be as efficient and selective as metal- or biocatalysis. Important discoveries in this area include novel Lewis base-catalyzed enantioselective processes and, more recently, simple Brønsted acid organocatalysts that rival the efficiency of traditional metal-based asymmetric Lewis acid-catalysts. Contributions to organocatalysis from our laboratories include several new and broadly useful concepts such as enamine catalysis and asymmetric counteranion-directed catalysis. Our laboratory has discovered the proline-catalyzed direct asymmetric intermolecular aldol reaction and introduced several other organocatalytic reactions.

有机催化是指使用低分子量催化剂的催化,其中金属不是催化原理或反应底物的一部分,它可以像金属催化或生物催化一样高效和选择性。该领域的重要发现包括新型Lewis碱催化的对映选择性过程,以及最近的简单Brønsted酸有机催化剂,其效率可与传统的金属基不对称Lewis酸催化剂相媲美。我们的实验室对有机催化的贡献包括一些新的和广泛有用的概念,如烯胺催化和不对称反阴离子定向催化。本实验室发现了脯氨酸催化的直接不对称分子间醛醇反应,并介绍了其他几种有机催化反应。
{"title":"New concepts for organocatalysis.","authors":"S C Pan,&nbsp;B List","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Organocatalysis, catalysis with low-molecular weight catalysts in which a metal is not part of the catalytic principle or the reaction substrate, can be as efficient and selective as metal- or biocatalysis. Important discoveries in this area include novel Lewis base-catalyzed enantioselective processes and, more recently, simple Brønsted acid organocatalysts that rival the efficiency of traditional metal-based asymmetric Lewis acid-catalysts. Contributions to organocatalysis from our laboratories include several new and broadly useful concepts such as enamine catalysis and asymmetric counteranion-directed catalysis. Our laboratory has discovered the proline-catalyzed direct asymmetric intermolecular aldol reaction and introduced several other organocatalytic reactions.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 2","pages":"1-43"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27552839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New developments in enantioselective Brønsted acid catalysis: chiral ion pair catalysis and beyond. 对映选择性Brønsted酸催化的新进展:手性离子对催化及其他。
M Rueping, E Sugiono

The design of catalytic reactions that proceed with high enantioselectivity is an important goal in organic synthesis. Increased interest in this research area has resulted in substantial progress, particularly in the field of metal catalyzed transformations. In recent years small organic molecules have been used as organocatalysts for a variety of enantioselective reactions. Among these, secondary amine catalysts are the most widely applied and can be used in the activation of the nucleophilic component through enamine formation (enamine catalysis), or by formation of an iminum intermediate to activate the electrophile (iminium catalysis). Additionally, chiral diols and thioureas, as well as carbene- and DMAP-derivatives (hydrogen bonding-, nucleophilic catalysis), have been shown to be versatile catalysts for enantioselective transformations. An alternative to these strategies is the activation of an electrophile or nucleophile by use of a chiral Brønsted acid. Compared to amino-, carbene-, pyridine- and hydrogen-bonding catalyzed transformations, enantioselective Brønsted acid catalysis has only recently emerged as important and promising area of research. In the course of our research program we were able to contribute significantly to the field of enantioselective Brønsted acid catalysis over the last 2 years, and could demonstrate for the first time that in various enantioselective transformations chiral Brønsted acid catalysts can give better or at least comparable results to metal-catalyzed processes. In this chapter we will highlight some of our most recent results and will, additionally, describe how we initially entered the field of asymmetric Brønsted acid catalysis by starting of from a biomimetic approach using nature as a role model.

设计具有高对映体选择性的催化反应是有机合成的一个重要目标。对这一研究领域的兴趣日益增加,特别是在金属催化转化领域取得了实质性进展。近年来,有机小分子已被用作各种对映选择性反应的有机催化剂。其中,仲胺催化剂应用最为广泛,可以通过形成烯胺(烯胺催化)激活亲核组分,也可以通过形成最小中间体激活亲电试剂(胺催化)。此外,手性二醇和硫脲,以及碳和dmap衍生物(氢键-亲核催化),已被证明是对映选择性转化的多功能催化剂。另一种方法是使用手性溴离子酸激活亲电试剂或亲核试剂。与氨基、羰基、吡啶和氢键催化的转化相比,对映选择性Brønsted酸催化是最近才出现的重要和有前途的研究领域。在过去两年的研究过程中,我们在对映选择性Brønsted酸催化领域做出了重大贡献,并首次证明了手性Brønsted酸催化剂在各种对映选择性转化中可以提供比金属催化过程更好或至少相当的结果。在本章中,我们将重点介绍我们的一些最新成果,并将描述我们如何最初进入不对称Brønsted酸催化领域,从仿生方法开始,以自然为榜样。
{"title":"New developments in enantioselective Brønsted acid catalysis: chiral ion pair catalysis and beyond.","authors":"M Rueping,&nbsp;E Sugiono","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The design of catalytic reactions that proceed with high enantioselectivity is an important goal in organic synthesis. Increased interest in this research area has resulted in substantial progress, particularly in the field of metal catalyzed transformations. In recent years small organic molecules have been used as organocatalysts for a variety of enantioselective reactions. Among these, secondary amine catalysts are the most widely applied and can be used in the activation of the nucleophilic component through enamine formation (enamine catalysis), or by formation of an iminum intermediate to activate the electrophile (iminium catalysis). Additionally, chiral diols and thioureas, as well as carbene- and DMAP-derivatives (hydrogen bonding-, nucleophilic catalysis), have been shown to be versatile catalysts for enantioselective transformations. An alternative to these strategies is the activation of an electrophile or nucleophile by use of a chiral Brønsted acid. Compared to amino-, carbene-, pyridine- and hydrogen-bonding catalyzed transformations, enantioselective Brønsted acid catalysis has only recently emerged as important and promising area of research. In the course of our research program we were able to contribute significantly to the field of enantioselective Brønsted acid catalysis over the last 2 years, and could demonstrate for the first time that in various enantioselective transformations chiral Brønsted acid catalysts can give better or at least comparable results to metal-catalyzed processes. In this chapter we will highlight some of our most recent results and will, additionally, describe how we initially entered the field of asymmetric Brønsted acid catalysis by starting of from a biomimetic approach using nature as a role model.</p>","PeriodicalId":87471,"journal":{"name":"Ernst Schering Foundation symposium proceedings","volume":" 2","pages":"207-53"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27547540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Ernst Schering Foundation symposium proceedings
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1