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Using metabolomics to monitor anticancer drugs. 利用代谢组学监测抗癌药物。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_089
Y-L Chung, J R Griffiths

The metabolome of a cancer cell is likely to show changes after responding to an anticancer drug. These changes could be used to decide whether to continue treatment or, in the context of a drug trial, to indicate whether the drug is working and perhaps its mechanism of action. (Nuclear) magnetic resonance spectroscopy (NMR/MRS) methods can offer important insights into novel anticancer agents in order to accelerate the drug development process including time-course studies on the effect of a drug on its site of action (termed pharmacodynamics), in this case the cancer. In addition, some classes of anticancer agents currently under development (e.g. antiangiogenics) are designed to be used in combination with other drugs and will not cause tumour shrinkage when used as single agents in Phase 1 clinical trials. Thus NMR/MRS may have a special role in monitoring the pharmacodynamic actions of such drugs in early-phase clinical trials. This review focuses on the use of ex vivo NMR and in vivo MRS methods for monitoring the effect of some novel anticancer drugs on the cancer metabolome. Ex vivo NMR methods are complementary to in vivo measurements, as they can provide additional information and help in the interpretation of the in vivo data.

在抗癌药物起作用后,癌细胞的代谢组可能会发生变化。这些变化可以用来决定是否继续治疗,或者在药物试验的背景下,表明药物是否有效,也许是其作用机制。核磁共振波谱(NMR/MRS)方法可以为新的抗癌药物提供重要的见解,以加速药物开发过程,包括药物对其作用部位(称为药效学)的影响的时间过程研究,在这种情况下是癌症。此外,目前正在开发的某些类型的抗癌药物(例如抗血管生成药物)被设计为与其他药物联合使用,并且在1期临床试验中作为单一药物使用时不会引起肿瘤缩小。因此,核磁共振/MRS可能在早期临床试验中监测此类药物的药效学作用方面具有特殊作用。本文综述了利用体外核磁共振和体内核磁共振技术监测一些新型抗癌药物对肿瘤代谢组的影响。离体核磁共振方法是对体内测量的补充,因为它们可以提供额外的信息,并有助于解释体内数据。
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引用次数: 26
N-heterocyclic carbenes: organocatalysts displaying diverse modes of action. n -杂环碳:显示不同作用模式的有机催化剂。
K Zeitler

Within the context of Lewis base catalysis N-heterocyclic carbenes represent an extremely versatile class of organocatalyst that allows for a great variety of different transformations. Starting from the early investigations on benzoin, and later Stetter reactions, the mechanistic diversity of N-heterocyclic carbenes, depending on their properties, has led to the development of several unprecedented catalytic reactions. This article will provide an overview of the versatile reactivity of N-heterocyclic carbenes.

在路易斯碱催化的背景下,n -杂环碳代表了一种非常通用的有机催化剂,可以实现各种不同的转化。从早期对安息香的研究开始,到后来的Stetter反应,n -杂环羰基的机理多样性,取决于它们的性质,导致了一些前所未有的催化反应的发展。本文将对n -杂环碳烯的多用途反应性进行综述。
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引用次数: 0
Progesterone signaling in mammary gland development. 乳腺发育中的孕激素信号。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_075
O. Conneely, B. Mulac‐Jeričević, R. Arnett-Mansfield
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引用次数: 33
Organocatalysis by hydrogen bonding networks. 氢键网络的有机催化。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_080
A. Berkessel
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引用次数: 3
Use of metabolic pathway flux information in anticancer drug design. 代谢途径通量信息在抗癌药物设计中的应用。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_094
L G Boros, T F Boros

The metabolic phenotype of tumor cells promote the proliferative state, which indicates that (a) cell transformation is associated with the activation of specific metabolic substrate channels toward nucleic acid synthesis and (b) increased expression phosphorylation, allosteric or transcriptional regulation of intermediary metabolic enzymes and their substrate availability together mediate unlimited growth. It is evident that cell transformation due to various K-ras point mutations is associated with the activation of specific metabolic substrate channels that increase glucose channeling toward nucleic acid synthesis. Therefore, phosphorylation, allosteric and transcriptional regulation of intermediary metabolic enzymes and their substrate availability together mediate cell transformation and growth. In this review, we summarize opposite changes in metabolic phenotypes induced by various cell-transforming agents, and tumor growth-inhibiting drugs or phytochemicals, or novel synthetic antileukemic drugs such as imatinib mesylate (Gleevec). Metabolic enzymes that further incite growth signaling pathways and thus promote malignant cell transformation serve as high-efficacy nongenetic novel targets for cancer therapies.

肿瘤细胞的代谢表型促进了增殖状态,这表明(a)细胞转化与核酸合成的特定代谢底物通道的激活有关;(b)中间代谢酶的表达磷酸化、变构或转录调节的增加以及它们的底物可用性共同介导无限生长。很明显,由于各种K-ras点突变引起的细胞转化与特定代谢底物通道的激活有关,这些通道增加了葡萄糖向核酸合成的通道。因此,中间代谢酶的磷酸化、变构和转录调控及其底物利用率共同介导细胞的转化和生长。在这篇综述中,我们总结了各种细胞转化剂,肿瘤生长抑制药物或植物化学物质,或新型合成抗白血病药物如甲酸伊马替尼(格列卫)诱导的代谢表型的相反变化。代谢酶进一步刺激生长信号通路,从而促进恶性细胞转化,是癌症治疗的高效非遗传新靶点。
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引用次数: 8
Sensing, presenting, and regulating PAMPs. 感知、呈现和调节PAMPs。
J L de Diego, G Gerold, A Zychlinsky

Recognition of microbial infection and initiation of immune responses are controlled by multiple mechanisms. Toll-like receptors (TLRs) are key components of the innate immune system that detect microbial infection. TLR activation helps to eliminate the invading pathogens, coordinate systemic defenses, and initiate adaptive immune responses. Despite progress elucidating the TLR signaling aspects and the physiological relevance of TLRs in microbial infections, the molecular basis of microbial recognition by TLRs is still not fully understood. In this article we focus on the availability of microbial ligands to regulate presentation to TLRs and assist in our understanding of TLR-mediated microbial recognition.

微生物感染的识别和免疫应答的启动由多种机制控制。toll样受体(TLRs)是先天免疫系统检测微生物感染的关键组成部分。激活TLR有助于消除入侵的病原体,协调系统防御,并启动适应性免疫反应。尽管在阐明TLR信号方面和TLR在微生物感染中的生理相关性方面取得了进展,但TLR对微生物识别的分子基础仍未完全了解。在这篇文章中,我们将重点关注微生物配体的可用性,以调节tlr的呈递,并帮助我们理解tlr介导的微生物识别。
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引用次数: 0
The role of diacylglycerol kinases in T cell anergy. 二酰甘油激酶在T细胞能量中的作用。
X P Zhong, B A Olenchock, G A Koretzky

Engagement of the T cell antigen receptor (TCR) results in the activation of multiple biochemical second messenger cascades that must be integrated for the appropriate T cell response. Once the critical TCR-stimulated signaling pathway is initiated by activation of protein tyrosine kinases, a series of adapter proteins is recruited that brings tyrosine-phosphorylated phospholipase Cgamma1 into the vicinity of its substrate, phosphatidylinositol-4,5-bisphosphate, resulting in the formation of two second messengers, inositol-1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). Previous work from multiple laboratories has shown that the balance between signals downstream of IP3 versus those downstream of DAG has profound effects on the fate of the stimulated T cells. In this report we summarize our recent data indicating that one key determinant of this balance of signals is the activity of members of the diacylglycerol kinase family, enzymes that convert DAG into phosphatidic acid.

T细胞抗原受体(TCR)的参与导致多个生化第二信使级联的激活,这些级联必须整合以实现适当的T细胞反应。一旦酪氨酸激酶激活了tcr刺激的关键信号通路,就会招募一系列适配器蛋白,将酪氨酸磷酸化的磷脂酶Cgamma1带到其底物磷脂酰肌醇4,5-二磷酸附近,从而形成两个第二信使,肌醇1,4,5-三磷酸(IP3)和二酰基甘油(DAG)。先前来自多个实验室的研究表明,IP3下游信号与DAG下游信号之间的平衡对受刺激T细胞的命运有着深远的影响。在这篇报告中,我们总结了我们最近的数据,表明信号平衡的一个关键决定因素是二酰基甘油激酶家族成员的活性,这些酶将DAG转化为磷脂酸。
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引用次数: 0
Fragment-based drug discovery using rational design. 使用合理设计的基于片段的药物发现。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2007_064
H Jhoti

Fragment-based drug discovery (FBDD) is established as an alternative approach to high-throughput screening for generating novel small molecule drug candidates. In FBDD, relatively small libraries of low molecular weight compounds (or fragments) are screened using sensitive biophysical techniques to detect their binding to the target protein. A lower absolute affinity of binding is expected from fragments, compared to much higher molecular weight hits detected by high-throughput screening, due to their reduced size and complexity. Through the use of iterative cycles of medicinal chemistry, ideally guided by three-dimensional structural data, it is often then relatively straightforward to optimize these weak binding fragment hits into potent and selective lead compounds. As with most other lead discovery methods there are two key components of FBDD; the detection technology and the compound library. In this review I outline the two main approaches used for detecting the binding of low affinity fragments and also some of the key principles that are used to generate a fragment library. In addition, I describe an example of how FBDD has led to the generation of a drug candidate that is now being tested in clinical trials for the treatment of cancer.

基于片段的药物发现(FBDD)是一种高通量筛选的替代方法,用于生成新的小分子候选药物。在FBDD中,使用灵敏的生物物理技术筛选相对较小的低分子量化合物(或片段)文库,以检测它们与目标蛋白的结合。与高通量筛选检测到的高分子量命中相比,由于碎片的尺寸和复杂性减小,预计其结合的绝对亲和力较低。通过使用药物化学的迭代循环,在三维结构数据的理想指导下,通常可以相对直接地将这些弱结合片段优化为有效和选择性的先导化合物。与大多数其他先导发现方法一样,FBDD有两个关键组成部分:检测技术及化合物库。在这篇综述中,我概述了用于检测低亲和力片段结合的两种主要方法,以及用于生成片段库的一些关键原则。此外,我还描述了一个例子,说明FBDD如何导致了一种候选药物的产生,这种候选药物目前正在进行癌症治疗的临床试验。
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引用次数: 20
Progesterone signaling in mammary gland development. 乳腺发育中的孕激素信号。
O M Conneely, B Mulac-Jericevic, R Arnett-Mansfield

The mammary gland undergoes extensive epithelial expansion and differentiation during pregnancy, leading ultimately to the development of functional milk-producing alveolar lobules. This phase of mammary gland remodeling is controlled primarily by the cooperative interplay between hormonal signals initiated by both progesterone and prolactin. Abrogation of mammary epithelial expression of receptors for either one of the hormones results in failure of alveologenesis and an absence of pregnancy-induced tertiary ductal side branches in the case of progesterone receptor-null (PRKO) mammary glands. By combining gene array approaches to identify PR- and prolactin (PRL)-dependent downstream signaling pathways and by using genetic mouse models to address the consequences of abrogation and/or misexpression of potential downstream genes, recent studies have begun to illuminate key signaling pathways that mediate the morphogenic effects of these hormones during pregnancy-induced mammary gland remodeling. Analysis of deregulated expression of PR-dependent gene transcripts in PRKO mammary glands has revealed that convergence between progesterone and prolactin signaling occurs in part through progesterone-dependent induction of mammary epithelial PRL receptors to prime the mammary epithelium to respond to PRL. Additional genes activated by PRs encode epithelial paracrine growth factor signals that regulate ductal and alveolar epithelial proliferation and survival, lineage-restricted transcription factors that control luminal and alveolar cell fate establishment and maintenance, and gap junction proteins that play a critical role in alveolar morphogenesis by establishment of epithelial cell polarity. Finally, two distinct isoforms of PRs (PR-A and PR-B) are coexpressed in the mammary gland and display extensively overlapping but partially distinct gene regulatory properties in relaying the progesterone signal.

在怀孕期间,乳腺经历了广泛的上皮细胞扩张和分化,最终导致功能性产奶的肺泡小叶的发育。乳腺重塑的这一阶段主要由孕激素和催乳素发起的激素信号之间的合作相互作用控制。在孕激素受体缺失(PRKO)乳腺的情况下,乳腺上皮中任一激素受体的表达缺失会导致肺泡形成失败和妊娠诱导的第三导管侧支缺失。通过结合基因阵列方法鉴定PR-和催乳素(PRL)依赖的下游信号通路,并通过使用遗传小鼠模型来解决潜在下游基因缺失和/或错误表达的后果,最近的研究已经开始阐明在妊娠诱导的乳腺重塑过程中介导这些激素形态发生作用的关键信号通路。对PRKO乳腺中pr依赖基因转录本表达失调的分析表明,孕激素和催乳素信号之间的趋同部分是通过孕激素依赖的乳腺上皮PRL受体诱导乳腺上皮对PRL作出反应而发生的。由PRs激活的其他基因编码调节导管和肺泡上皮增殖和存活的上皮旁分泌生长因子信号,控制管腔和肺泡细胞命运建立和维持的谱系限制性转录因子,以及通过上皮细胞极性的建立在肺泡形态发生中起关键作用的间隙连接蛋白。最后,pr的两种不同亚型(PR-A和PR-B)在乳腺中共表达,并在传递孕激素信号中表现出广泛重叠但部分不同的基因调控特性。
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引用次数: 0
Mammary development, carcinomas and progesterone: role of Wnt signalling. 乳腺发育、癌症和黄体酮:Wnt信号的作用。
Pub Date : 2007-01-01 DOI: 10.1007/2789_2008_074
R. Lamb, H. Harrison, R. Clarke
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引用次数: 15
期刊
Ernst Schering Foundation symposium proceedings
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