Aim: A HPLC method was developed and validated for the novel combination of rutin (RN) and donepezil (DNP). Materials & methods: RN and DNP were simultaneously eluted through a C18 column (Ø 150 × 4.6 mm) with a 60:40 v/v ratio of 0.1% formic acid aqueous solution to methanol at 0.5 ml/min. Results: The purposed method was found linear, selective, reproducible, accurate and precise with percent RSD less than 2. The limit of quantification for RN and DNP was found 3.66 and 3.25 μg/ml, respectively. Conclusion: Validated as per the ICH guidelines, the developed method efficiently quantified RN and DNP co-loaded in DQAsomes (121 nm) estimating matrix effect, release profile, entrapment efficiency, loading efficiency and in vivo plasma kinetics.
{"title":"Simultaneous estimation of rutin and donepezil through RP-HPLC: implication in pharmaceutical and biological samples.","authors":"Rafquat Rana, Keerti Mishra, Shourya Tripathi, Animesh Kumar Gupta, Amrendra Kumar Tiwari, Pavan Kumar Yadav, Abhiram Kumar, Jvus Chakradhar, Sanjay Singh, Sonia Verma, Pooja Yadav, Manish K Chourasia","doi":"10.1080/17576180.2024.2344395","DOIUrl":"10.1080/17576180.2024.2344395","url":null,"abstract":"<p><p><b>Aim:</b> A HPLC method was developed and validated for the novel combination of rutin (RN) and donepezil (DNP). <b>Materials & methods:</b> RN and DNP were simultaneously eluted through a C18 column (Ø 150 × 4.6 mm) with a 60:40 v/v ratio of 0.1% formic acid aqueous solution to methanol at 0.5 ml/min. <b>Results:</b> The purposed method was found linear, selective, reproducible, accurate and precise with percent RSD less than 2. The limit of quantification for RN and DNP was found 3.66 and 3.25 μg/ml, respectively. <b>Conclusion:</b> Validated as per the ICH guidelines, the developed method efficiently quantified RN and DNP co-loaded in DQAsomes (121 nm) estimating matrix effect, release profile, entrapment efficiency, loading efficiency and <i>in vivo</i> plasma kinetics.</p>","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11299792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-22DOI: 10.1080/17576180.2024.2349423
Anish Khan, Nisha Khan, Raj Singh
{"title":"Tuberculosis diagnosis versus GeneXpert\u0000 ®\u0000 MTB/RIF formats","authors":"Anish Khan, Nisha Khan, Raj Singh","doi":"10.1080/17576180.2024.2349423","DOIUrl":"https://doi.org/10.1080/17576180.2024.2349423","url":null,"abstract":"","PeriodicalId":8797,"journal":{"name":"Bioanalysis","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141109492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}