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Communication and its disorders: Definition and taxonomy from a phoniatric perspective 沟通及其障碍:从语音学角度的定义和分类
Pub Date : 2010-11-24 DOI: 10.3109/1651386X.2010.530023
A. Schindler, G. Ruoppolo, Umberto Barillari
Abstract Objective: The aim of the paper is to describe communication sciences and disorders from a phoniatric perspective, i.e. from the point of view of medical doctors involved in the rehabilitation management of a communication disorder. Communication: Communication is a complex behaviour, combining physical and mental events, with the aim of exchanging messages between two or more individuals. Communication is the execution of a task by an individual in his daily life. Different body structures and functions, as well as environmental factors, strongly impact on communication and related activities. Communication disorders: A communication disorder is an impairment in sending and/or receiving a message; it could be the consequence of a disease, a treatment or an environmental situation. There are several professions, both medical and non-medical, involved in the management of patients with a communication disorder. The phoniatrician is the only medical doctor whose focus is the diagnosis and rehabilitation of communication disorders. Taxonomy of communication disorders: There are several diseases, conditions and situations that can lead to a limitation and/or restriction in communication. A list of diseases would be misleading in describing possible communication disorders; it might be more useful to offer a framework of the impaired functions that may lead to a communication disorder. A communication disorder taxonomy based on the direct and indirect impairment of voice, speech, language and hearing functions is presented. Conclusion: The presented taxonomy may contribute to the assessment and management of patients with communication disorders and represents a framework for clinical research in the different disciplines involved in the large field of communication science and disorders.
摘要目的:本文的目的是从语音学的角度,即从参与沟通障碍康复管理的医生的角度来描述沟通科学和障碍。沟通:沟通是一种复杂的行为,结合了身体和心理事件,目的是在两个或更多人之间交换信息。沟通是个体在日常生活中执行任务的过程。不同的身体结构和功能以及环境因素对交流和相关活动有很大的影响。沟通障碍:沟通障碍是发送和/或接收信息的障碍;它可能是疾病、治疗或环境状况的结果。有几个专业,包括医疗和非医疗,涉及到沟通障碍患者的管理。语音医生是唯一专注于诊断和康复沟通障碍的医生。交流障碍的分类:有几种疾病、状况和情况会导致交流受到限制和/或限制。在描述可能出现的沟通障碍时,列出一系列疾病会产生误导;提供一个可能导致沟通障碍的功能受损的框架可能会更有用。提出了一种基于语音、言语、语言和听力功能直接和间接损害的沟通障碍分类方法。结论:该分类有助于沟通障碍患者的评估和管理,并为沟通科学与障碍大领域中不同学科的临床研究提供了一个框架。
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引用次数: 10
Communication disorders in the elderly 老年人的沟通障碍
Pub Date : 2010-11-24 DOI: 10.3109/1651386X.2010.522859
A. am Zehnhoff-Dinnesen, W. Angerstein, D. Deuster
Abstract The percentage of elderly individuals in the general population is increasing because of rising average life expectancy. Elderly people today enjoy much more active and more communicative lives than previous generations. However, due to age related changes and the increasing prevalence of diseases affecting phonation, articulation, respiration, speech, deglutition and their neurological regulation mechanisms – dysphonia, dysarthria, aphasia/dysphasia and dysphagia, they are becoming more frequent in this population group. These communication disorders can cause social isolation, anxiety and depression. Patients and family members alike suffer from the negative impact these disorders have on communication and quality of life. The prevalence of age related voice problems in the elderly is reported to be as high as 20–47%, for speech and/or language problems 70–75%, and up to 40% for dysphagia. There is a growing need for effective therapeutic approaches to communication disorders in the elderly. Physiological age related changes, the causes of communication disorders in the elderly, their impact on daily life, and rehabilitative options are presented.
由于平均预期寿命的增加,老年人在总人口中的比例正在增加。今天的老年人比前几代人享受着更活跃、更健谈的生活。然而,由于年龄相关的变化和影响发音、发音、呼吸、言语、吞咽及其神经调节机制的疾病的日益流行——发音困难、构音障碍、失语/吞咽困难和吞咽困难,它们在这一人群中变得越来越常见。这些沟通障碍会导致社会孤立、焦虑和抑郁。患者和家庭成员同样遭受这些障碍对沟通和生活质量的负面影响。据报道,老年人中与年龄相关的声音问题的患病率高达20-47%,言语和/或语言问题的患病率为70-75%,吞咽困难的患病率高达40%。对老年人沟通障碍的有效治疗方法的需求日益增长。生理年龄相关的变化,老年人沟通障碍的原因,他们对日常生活的影响,和康复方案提出。
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引用次数: 5
The phoniatric vision on communication and its disorders 交流及其障碍的语音视觉
Pub Date : 2010-11-24 DOI: 10.3109/1651386X.2010.531547
A. Schindler
Audiological Medicine is a journal that aims to provide a forum for physicians working in the fi eld of hearing, balance and communication disorders. In the last seven years several issues of the journal have been devoted to hearing or balance disorders, and several papers dealing with hearing and/or balance physiology, pathology and treatment have been published. This is the fi rst issue entirely devoted to communication and its disorders; the authors contributing to this issue belong primarily to phoniatrics, the medical speciality of communication (and swallowing) disorders. This issue represents a further step in the collaboration between audiology and phoniatrics, two medical specialities with several commonalities. In the history of several countries, such as Italy and Mexico (1,2), audiology and phoniatrics were originally two branches of a common fi eld; also, in many European countries, such as Germany, Italy and Poland, these two specialities are combined. The two most important international societies in audiology and phoniatrics, the International Association of Physicians in Audiology (IAPA) and the Union of the European Phoniatricians (UEP), have increased their cooperation in recent years, trying to support each other (3). A journal publishing papers of interest for both audiology and phoniatrics could be a further goal in this cooperation process. The aim of this issue of Audiological Medicine is two-fold: to provide a framework for communication disorders and to give a glimpse on areas of clinical research in this fi eld. In order to achieve this second goal, review articles as well as research papers are presented on issues of the entire age spectrum: papers on verbal communication, both oral and written, as well as non-verbal communications have been included. This issue of Audiological Medicine starts with a paper providing a broad vision of communication and its disorders, together with taxonomic systems. After a brief analysis of the human communication key concepts, the structures, functions, activities, participation and environmental factors contributing the most to communication are described; the ICF (International Classifi cation of Functioning, Disability and Health) terms and defi nitions are used, with the aim to reduce terminology inconsistency. Finally, the classifi cation of communications disorders from a phoniatrics perspective is reported, attempting to give a vision of the entire fi eld. A research paper by Woisard and Lapage on the differences and commonalities between intelligibility and severity of impaired speech highlights the impact of a speech disorder on the listener. The paper contributes to a clearer defi nition of the following concepts: severity, intelligibility and comprehensibility of speech disorders. In the paper of van Opstal et al., the relationship between stressed personality and perceived handicap in voice disordered patients is analysed; this is an example of the impact of voice disorder
《听力学医学》是一本旨在为在听力、平衡和沟通障碍领域工作的医生提供论坛的杂志。在过去的七年中,该杂志的几期专门讨论听力或平衡障碍,并发表了几篇关于听力和/或平衡生理学,病理学和治疗的论文。这是第一期专门讨论沟通及其障碍的杂志;对这一问题作出贡献的作者主要属于语音病学,交流(和吞咽)障碍的医学专业。这一问题代表了听力学和语音病学这两个具有若干共性的医学专业之间合作的又一步。在一些国家的历史上,如意大利和墨西哥(1,2),听力学和语音病学最初是一个共同领域的两个分支;此外,在许多欧洲国家,如德国、意大利和波兰,这两种专业是结合在一起的。国际听力学医师协会(IAPA)和欧洲听力学医师联盟(UEP)这两个最重要的国际听力学和语音病学协会近年来加强了合作,试图相互支持(3)。在这一合作过程中,一个发表听力学和语音病学相关论文的期刊可能是进一步的目标。本期《听力学医学》的目的有两个方面:提供交流障碍的框架,并对该领域的临床研究领域进行一瞥。为了实现第二个目标,综述文章和研究论文都是关于整个年龄范围的问题:关于口头和书面语言交流的论文,以及非语言交流的论文都被包括在内。本期《听力学医学》以一篇论文开篇,提供了交流及其障碍的广泛视野,以及分类系统。在简要分析人类交际的关键概念后,阐述了对交际影响最大的结构、功能、活动、参与和环境因素;使用ICF(国际功能、残疾和健康分类)术语和定义,目的是减少术语的不一致。最后,通讯障碍的分类从语音病学的角度被报道,试图给整个领域的愿景。Woisard和Lapage的一篇关于言语障碍的可理解性和严重程度之间的差异和共性的研究论文强调了言语障碍对听者的影响。本文对言语障碍的严重性、可理解性和可理解性等概念进行了较为清晰的界定。van Opstal等人的论文分析了语音障碍患者应激人格与感知障碍之间的关系;这是声音障碍对生活质量影响的一个例子,这是语音病学领域日益发展的一个领域。生活在西方国家的人口年龄的增长是卫生系统组织的主要问题;am Zehnhoff-Dinnesen等人的综述从语音学的角度总结了导致老年人交流受限的功能受损。这不仅是临床研究的一个高度关注的领域,也是卫生管理人员的一个高度关注的领域。在许多论文(2,3)中,已经认识到儿童耳聋是语音病学和听力学医学重叠的领域,2010;8: 161 - 162
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引用次数: 0
Role of self-induced sound therapy: Bhramari Pranayama in Tinnitus 自我诱导声疗法的作用:调息在耳鸣中的作用
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.489694
Sidheshwar Pandey, N. Mahato, Ravishankar Navale
Absract Objectives: Treatment of tinnitus is not ‘absolute’ in terms of its approach through a single therapy model. The more recent modes of treatment focus on attenuating somatic perception and on synchronizing the emotional component of tinnitus with more ‘positive’ physiological events in the body, so that the person does not correlate the presence of tinnitus with annoyance and a source of disturbance. Both these goals are possible with neurophysiological ‘habituation’ at proper synapses across the auditory pathway. The present study has been conducted to observe the effects of Bhramari Pranayama (BP) on both the physical and emotional aspects of tinnitus. Bhramari Pranayama is a ‘Yogic’ technique that involves the combination of a relaxing posture and a process of producing sub-tinnitus level humming sound during exhalation along with simultaneous pressing of the closed eyelids. Study Design: A group of patients with tinnitus was administered BP as a therapy. Three other groups of similar patients were given Ginkgo biloba, Masking therapy and a combination of all the above-mentioned modalities, respectively, as treatment for tinnitus. The outcome of the study was determined by analysing the pre- and post-therapeutic values measured for parameters such as: 1) Loudness; 2) THI score; and 3) Anxiety and Depression scale. Results: Demonstrated that BP as well as all the other modalities of treatment significantly reduced the post-therapeutic scores in all the parameters, in all the groups. Conclusion: We concluded that BP significantly reduced the irritability, depression and the anxiety associated with tinnitus. It relieved the symptoms in tinnitus possibly by 1) acting as source of self-generated sound; 2) inducing parasympathetic predominance in the neural milieu; and 3) by acting as a relaxation technique. BP may serve as a cost effective, frequently applicable adjuvant therapy for tinnitus that probably acts through neuromodulating principles.
摘要:目的:耳鸣的治疗方法不是“绝对的”,通过单一的治疗模式。最近的治疗模式侧重于减弱身体感知,并将耳鸣的情绪成分与体内更“积极”的生理事件同步,这样人们就不会将耳鸣的存在与烦恼和干扰源联系起来。这两个目标都可以通过听觉通路上适当突触的神经生理“习惯化”来实现。本研究旨在观察Bhramari Pranayama (BP)对耳鸣生理和情绪方面的影响。Bhramari Pranayama是一种“瑜伽”技巧,包括放松的姿势和在呼气时产生亚耳鸣水平的嗡嗡声的过程,同时按压紧闭的眼睑。研究设计:一组耳鸣患者给予BP治疗。另外三组相似的患者分别给予银杏叶、掩蔽疗法和上述所有方式的组合治疗耳鸣。研究结果是通过分析治疗前和治疗后的参数值来确定的:1)响度;2) THI分数;3)焦虑抑郁量表。结果:在所有组中,BP以及所有其他治疗方式均显著降低了所有参数的治疗后评分。结论:BP可显著减轻耳鸣患者的烦躁、抑郁和焦虑。它减轻耳鸣症状可能是由于1)作为自发声源;2)在神经环境中诱导副交感神经优势;3)通过表演作为一种放松技巧。BP可能是一种经济有效的耳鸣辅助治疗方法,可能通过神经调节原理起作用。
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引用次数: 18
The inner ear phenotype of Volchok (Vlk): An ENU-induced mouse model for CHARGE syndrome Volchok (Vlk)内耳表型:enu诱导的CHARGE综合征小鼠模型
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.490039
Danielle R. Lenz, A. Dror, G. Wekselman, H. Fuchs, M. D. de Angelis, K. Avraham
Abstract Objective: The Volchok (Vlk) mouse originated as a part of a large-scale ENU program on a C3HeB/FeJ background. The mice present a dominant pattern of inheritance reflected mainly by circling activity, which led us to search for the causative mutation underlying the vestibular phenotype. Study design: Linkage analysis and subsequent sequencing were used to narrow down the genomic region and uncover the mutation, respectively. Behavioral analysis and examination of the inner ear phenotype was performed using the modified SHIRPA protocol, auditory brainstem response (ABR), immunofluorescence, light microscopy, scanning electron microscopy (SEM), paint-fill and neurofilament staining techniques. Results: A nonsense mutation was discovered in the Chd7 gene responsible for CHARGE syndrome in humans, which leads to a decrease in Chd7 expression in the nuclei of the vestibular and cochlear cells and structural abnormalities in the inner ear. A mild hearing loss was found in the mutant mice. The malformations detected include a truncated lateral semicircular canal and smaller lateral crista, a decrease in size or absence of the round window in the cochlea and lack of innervations to the posterior crista. Conclusion: Vlk joins a series of previously characterized mice bearing a Chd7 mutation, linking abnormalities found both in ENU-induced mutants and a knock-out mouse model. Combining the information from multiple Chd7 alleles provides a more comprehensive understanding of the mechanisms underlying this human disease.
目的:Volchok (Vlk)小鼠起源于C3HeB/FeJ背景下的大规模ENU项目的一部分。小鼠表现出主要通过循环活动反映的显性遗传模式,这促使我们寻找前庭表型下的致病突变。研究设计:分别使用连锁分析和随后的测序来缩小基因组区域并揭示突变。采用改进的SHIRPA方案,听觉脑干反应(ABR),免疫荧光,光学显微镜,扫描电镜(SEM),油漆填充和神经丝染色技术进行内耳表型的行为分析和检查。结果:在人类CHARGE综合征的Chd7基因中发现了一个无义突变,导致前庭和耳蜗细胞细胞核中Chd7表达减少,内耳结构异常。在突变小鼠中发现了轻微的听力损失。检测到的畸形包括外侧半规管截短、外侧嵴变小、耳蜗圆窗缩小或缺失、后嵴缺乏神经支配。结论:Vlk加入了一系列先前表征的携带Chd7突变的小鼠,将在enu诱导突变体和敲除小鼠模型中发现的异常联系起来。结合来自多个Chd7等位基因的信息,可以更全面地了解这种人类疾病的潜在机制。
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引用次数: 4
Hearing profile in hepatitis C virus patients under dual treatment with interferon and ribavirin 干扰素和利巴韦林双重治疗丙型肝炎患者的听力状况
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.515708
M. Shabana, Ayman Amer, A. Dabbous, Amani Ahmed Al-Sunni
Abstract Objective: To study the effect of dual therapy with interferon and ribavirin on hearing in chronic HCV patients treated with these medications. Study design: This was a cross-sectional study in which assessment of hearing and cochlear function was carried out using pure tone audiometry and transient otoacoustic emissions (TEOAEs). Thirty young-aged adult patients with chronic active hepatitis C virus (HCV) received dual treatment with interferon and ribavirin for at least three months. They were compared to 30 chronic HCV patients for whom treatment was not applicable. Results: HCV patients under treatment exhibited a mild high-frequency sensorineural hearing loss (SNHL) in 63.3% of subjects (unilateral in 36.7% and bilateral in 26.7% of subjects). HCV patients not receiving treatment showed SNHL, which was unilateral in 13.3% of subjects. In HCV patients under treatment 33% of subjects had a bilateral TEOAE pass and 30% of subjects had a unilateral pass (unilateral partial pass). The remaining 37% of subjects had a bilateral partial pass, compared to a bilateral pass in 96.67% of subjects not receiving treatment. There was a statistically significant difference between the two groups with regard to the hearing threshold at different frequencies and TEOAE overall wave reproducibility in both ears. Normal hearing ears of those with a unilateral SNHL showed a lower pass rate (27.3%) than those of the bilateral normal hearing group (54.5%), which suggests subtle changes in their cochleae. Conclusion: Dual interferon and ribavirin therapy for HCV patients could damage the cochlear outer or inner hair cells.
摘要目的:探讨干扰素和利巴韦林双重治疗对慢性HCV患者听力的影响。研究设计:这是一项横断面研究,使用纯音测听和瞬态耳声发射(teoae)来评估听力和耳蜗功能。30例患有慢性活动性丙型肝炎病毒(HCV)的年轻成人患者接受干扰素和利巴韦林的双重治疗至少三个月。他们与30名不适用治疗的慢性HCV患者进行了比较。结果:在接受治疗的HCV患者中,63.3%的受试者表现出轻度高频感音神经性听力损失(SNHL)(单侧36.7%,双侧26.7%)。未接受治疗的HCV患者出现单侧SNHL,占13.3%。在接受治疗的HCV患者中,33%的受试者双侧TEOAE通过,30%的受试者单侧TEOAE通过(单侧部分通过)。其余37%的受试者双侧部分通过,而未接受治疗的受试者双侧通过的比例为96.67%。两组在不同频率下的听阈值及双耳TEOAE总波再现性差异均有统计学意义。单侧SNHL患者听力正常耳的通过率(27.3%)低于双侧听力正常组(54.5%),提示耳蜗有细微变化。结论:干扰素联合利巴韦林双药治疗HCV患者可损伤耳蜗外毛细胞或内毛细胞。
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引用次数: 1
Basic audiology for medical students 医学生基础听力学
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.499708
P. Berruecos
Abstract Delay in the identification, diagnosis and intervention of hearing impairment and deafness is mainly linked to the lack of knowledge and commitment of medical doctors (MDs) towards this impairment. In 1997 and in 2006, a multicentre study on the status of prevention of hearing impairment (HI) and deafness in Mexico, directed towards the parents of deaf children, clearly showed this. Similar findings were obtained with a 10-item questionnaire designed to evaluate knowledge and attitudes towards hearing loss (HL) and used with those MDs selected in the annual national examination for the selection of candidates for residencies. From 23,482 applicants, 5464 MDs – the elite of general practitioners (GPs) who had graduated from more than 70 medical schools all over the country – were selected to start their specialization in 2007, and from these a total of 2727 completed questionnaires (49.9%) were obtained. These again showed a very low level of knowledge and misguided attitudes towards hearing, deafness and its consequences. In addition to efforts centred upon the postgraduate four-year residency in ‘Audiology, Otoneurology and Phoniatrics’ (Faculty of Medicine, National University of Mexico), we proposed inclusion of an audiology module in medical students’ clinical training and the undergraduate rotatory internship. In 2008, 20 multiple-choice questions were administered to 151 students at the beginning and end of the module. The comparison of these evaluations showed an initial average of correct responses of 33.4% and a final one of 77.9%, with a clear improvement of 44.5%. It is proposed to disseminate the module from our university, the most important in the country, to all the medical schools in Mexico, in order to improve the identification, diagnosis and intervention skills of future MDs.
听力障碍和耳聋的识别、诊断和干预的延迟主要与医生(md)对这种障碍缺乏知识和承诺有关。1997年和2006年,墨西哥针对失聪儿童的父母开展了一项多中心研究,研究听力损伤和耳聋的预防状况,结果清楚地表明了这一点。一份包含10个项目的问卷用于评估对听力损失(HL)的知识和态度,并用于参加年度全国住院医师选拔考试的医学博士,也得到了类似的结果。从23,482名申请者中,5464名医学博士——从全国70多所医学院毕业的全科医生精英——被选中于2007年开始他们的专业研究,从中总共获得了2727份完成的问卷(49.9%)。这再次表明,他们对听力、耳聋及其后果的认识水平很低,态度也很错误。除了在“听力学、耳神经学和听力病学”(墨西哥国立大学医学院)的研究生四年住院医师培训上所做的努力外,我们还建议在医学生的临床培训和本科生轮岗实习中纳入听力学模块。2008年,151名学生在考试模块的开始和结束时被要求回答20道选择题。这些评价的比较表明,初始平均正确率为33.4%,最终平均正确率为77.9%,明显提高了44.5%。建议将我国最重要的大学的模块推广到墨西哥所有医学院,以提高未来医学博士的识别、诊断和干预技能。
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引用次数: 0
Speech-evoked auditory potentials in attention deficit hyperactivity disorder 注意缺陷多动障碍的言语诱发听觉电位
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.499738
Hannan Azzam, D. Hassan
Abstract Objective: To explore the processing of auditory information through auditory brainstem and higher cortical regions in a sample of children with attention deficit hyperactivity disorder (ADHD) of the combined inattention and hyperactivity subtype (ADHD/C) using auditory evoked potentials. Study Design: Fifteen Arabic speaking ADHD/C children were compared to 15 age-matched normal controls, aged between five and ten years. A Mini-International Neuropsychiatric Interview for Children and Adolescents (Arabic version) was performed to confirm the diagnosis, subtype of ADHD and to exclude comorbid conditions. All children were subjected to Auditory Brainstem Evoked Response (ABR) to click, speech ABR and cortical Mis-Match Negativity (MMN) to speech stimuli. Results: Thirty-three percent of the ADHD/C group showed prolongation of absolute and/or inter-peak wave latencies of the click ABR beyond two standard deviations. The peak latency of the onset waves for speech ABR was abnormal in 87% of the study group. Only three ADHD/C children gave normal MMN. Conclusion: This study demonstrated that abnormal auditory brainstem timing with reduced cortical functions was a characteristic in the studied ADHD/C children. Speech ABR can serve as a biological marker for brainstem neural asynchrony in children with ADHD/C. It might help to organize the highly heterogeneous population of ADHD into more homogeneous subgroups, at least on a particular biological indicator, specifically in individuals who cannot perform the behavioural measures. Further research is needed to determine whether individuals within these subgroups share a similar auditory perceptual profile.
摘要目的:利用听觉诱发电位探讨注意缺陷多动障碍(ADHD/C)合并不注意多动亚型(ADHD/C)患儿听觉脑干及皮层高级区对听觉信息的加工。研究设计:将15名说阿拉伯语的ADHD/C儿童与15名年龄匹配的正常对照进行比较,年龄在5到10岁之间。对儿童和青少年进行了小型国际神经精神病学访谈(阿拉伯语版),以确认ADHD的诊断、亚型和排除合并症。所有儿童对声音刺激进行听觉脑干诱发反应(ABR),对言语刺激进行听觉脑干诱发反应(ABR),对言语刺激进行皮层错配负性反应(MMN)。结果:33%的ADHD/C组显示click ABR的绝对和/或峰间波潜伏期延长超过两个标准差。言语ABR发作波的峰潜伏期在87%的研究组中出现异常。只有3例ADHD/C患儿MMN正常。结论:本研究表明,听觉脑干时间异常伴皮层功能减少是ADHD/C儿童的一个特征。言语ABR可作为ADHD/C患儿脑干神经非同步的生物学标志物。它可能有助于将高度异质性的ADHD人群组织成更同质的亚组,至少在特定的生物学指标上,特别是在不能进行行为测量的个体中。需要进一步的研究来确定这些亚群中的个体是否具有相似的听觉感知特征。
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引用次数: 5
Three-dimensional confocal microscopy of the mammalian inner ear 哺乳动物内耳的三维共聚焦显微镜
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.502301
Glen Macdonald, E. Rubel
Abstract Objective: Control the refractive index in fixed specimens of mammalian inner ear to reduce spherical aberrations that limit our ability to obtain 3-dimensional images of fluorescently labeled inner ear specimens by conventional laser scanning confocal microscopy. Study Design: Mouse inner ear specimens were fixed with minimal dissection, rapidly decalcified and fluorescently labeled by immunohistochemistry then impregnated by epoxy resin or a clearing agent composed of 5 parts methyl salicylate:3 parts benzyl benzoate. The specimens were imaged by both confocal microscopy and by widefield epi-fluorescent microscopy, with additional processing by deconvolution. Results: Rapid decalcification preserved tissue morphology and antigenicity for the antibodies tested. Although the epoxy allowed some reduction of spherical aberration, the clearing agent enabled optical volumes of high quality and resolution to be collected through the inner ear. The conditions for immunolabeling are important to ensure adequate perfusion of the immuno-labeling reagents throughout the specimen. Conclusion: Spherical aberration reduces signal intensity, contrast and resolution in optical microscopy. Creating a homogeneous refractive index throughout the inner ear to reduce spherical aberration allowed optical volumes to be collected through an intact, fluorescently labeled cochlea in a manner limited by the working distance of the objective lens rather than by spherical aberration. Optical volumes collected by this method from the mammalian inner ear promise to be useful for applications such as tracing innervation patterns, counting sensory cells or other structures over large regions of the sensory epithelium, and characterization of the inner ear in animal models of human deafness disorders.
摘要:目的:控制哺乳动物内耳固定标本的折射率,以减少限制我们通过常规激光扫描共聚焦显微镜获得荧光标记内耳标本三维图像的球差。研究设计:小鼠内耳标本经最小剥离固定,快速脱钙,免疫组织化学荧光标记,然后用环氧树脂或由5份水杨酸甲酯和3份苯甲酸苄酯组成的清除剂浸渍。通过共聚焦显微镜和宽视场外延荧光显微镜对标本进行成像,并通过反褶积进行额外处理。结果:快速脱钙保存组织形态及抗体抗原性检测。虽然环氧树脂可以减少一些球差,但清除剂可以通过内耳收集高质量和分辨率的光学体积。免疫标记的条件很重要,以确保免疫标记试剂在整个标本中充分灌注。结论:球差降低了光学显微镜的信号强度、对比度和分辨率。在整个内耳中创造一个均匀的折射率来减少球差,这样就可以通过一个完整的、荧光标记的耳蜗收集光学体积,这种方式受物镜工作距离的限制,而不是受球差的限制。通过这种方法从哺乳动物内耳中收集的光学体积有望用于诸如追踪神经支配模式,计数感觉细胞或感觉上皮大区域的其他结构以及人类耳聋疾病动物模型的内耳表征等应用。
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引用次数: 9
Audiology and phoniatrics: Vision from Mexico of its origins, relationships, fields and boundaries 听力学和语音病学:从墨西哥看它的起源、关系、领域和边界
Pub Date : 2010-09-10 DOI: 10.3109/1651386X.2010.503568
P. Berruecos
A long time ago, a French Professor (Sebileau or Terracol) wrote that otorhinolaryngology (ORL) was like a nebula of unsteady origin and imprecise trajectory. At the very beginning of this speciality, general surgeons practised tracheotomy or laryngectomy, and it is impossible to forget the wide tracks opened by a non-medical doctor, Manuel Garcia, not only to the larynx but also to other anatomical areas. Nevertheless, somebody suddenly said “ ear, nose and throat ” and someone else, more ambitious, added “ eye, ear, nose and throat ” . The organ based criteria include some related areas and one odd addition and, only many years later, was it realized that the only relationship between the eyes and nose was a pair of small ducts by which infection travels up or tears go down. In Mexico, the separation of the specialities involved in the Mexican Society of Ophthalmology and ORL took place in 1947. After that, the evolution of ENT was dynamic and generated new specialties: 1) bronchoesophagology, with Killian and Jackson and, as a natural consequence, anaesthesiology; 2) plastic surgery, to treat outer ear, nose and lip abnormalities; 3) maxillofacial surgery; 4) oncology surgery linked with laryngeal carcinoma; and 5) allergy, born after the awareness that the nose must not be the object of mutilating surgery. It is not irrelevant to remember that George Laurens, speaking against the very frequent nasal septum surgery at the time described “ the septum that must be vertical but is terrifi ed by the vertical ” . In ORL evolution, Lempert in 1938 and then Rosen, Shea and other ENT specialists developed several surgical techniques to treat some types of
很久以前,一位法国教授(Sebileau或Terracol)写道,耳鼻喉科(ORL)就像一个起源不稳定、轨迹不精确的星云。在这一专业刚开始的时候,普通外科医生施行气管切开术或喉部切除术,人们不可能忘记Manuel Garcia这名非内科医生不仅对喉部而且对其他解剖部位开了宽阔的手术道。然而,突然有人说“耳朵、鼻子和喉咙”,还有人更有野心地说“眼睛、耳朵、鼻子和喉咙”。以器官为基础的标准包括一些相关区域和一个奇怪的补充,直到许多年后,人们才意识到,眼睛和鼻子之间的唯一关系是一对小导管,感染通过它们向上传播,眼泪通过它们向下流。在墨西哥,1947年,墨西哥眼科学会和ORL所涉及的专业分离。在那之后,耳鼻喉科的发展是动态的,并产生了新的专业:1)支气管食管科,有基利安和杰克逊,自然而然地,麻醉科;2)整形手术,治疗外耳、鼻、唇畸形;3)颌面外科;4)喉癌相关肿瘤手术;5)过敏,出生后意识到鼻子绝不能成为残害手术的对象。我们应该记得乔治·劳伦斯,在反对频繁的鼻中隔手术时曾说过"鼻中隔必须是垂直的但又害怕垂直"在ORL的发展中,Lempert在1938年,然后Rosen, Shea和其他耳鼻喉科专家开发了几种手术技术来治疗某些类型
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引用次数: 1
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Audiological medicine
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