Pub Date : 2019-01-29DOI: 10.11648/J.IJGG.20180604.12
A. Amin, K. Iftekharuddaula, A. Sarker, A. Talukder, Sharmishtha Ghoshal, A. Shalahuddin, T. Aditya, Ansar Ali, B. Collard
Bangladesh had harbored numerous submergence tolerant rice local cultivars or landraces (popularly known as Joli Aman). As the local cultivars endure intervallic flash-flooding year after year, it is presumed that genes/Quantitative Trait Loci (QTLs) that give resilience are probably going to be available in these genotypes. In submergence screening, the performance of three tested accessions (Lakhi, Atshotti and Damsi) demonstrated them to be as tolerant as the check FR13A. Other germplasm accessions (DSL-78-8, Laldepa, Putidepa, Laxmi digha) performed great recuperation capacity after de-submergence. Damsi, Rajasail, Haloi, Lal Digha, Manik Digha, Bhawalia, DSL-78-8, Putidepa, Atshotti and Jalda IRRI don't have the SUB1 QTL and furthermore not like the BR5, a submergence susceptible check variety. Bajal, Horkoach, Pathornuti, Laxmi Digha, Horinga Digha, Bhawalia Digha and BR5 was not amplified by Gns2 conferring SUB1 QTL (submergence tolerance specific QTL). Significantly, the local cultivars i.e. DSL-78-8, Putidepa and Sadadanga boro were identified as having better submergence tolerance however having no resistant alleles of GnS2 and Sub1C173, indel markers specific to SUB1 QTL. The result of molecular screening revealed new submergence tolerant rice cultivars which do not possess SUB1 QTL. To discover new submergence tolerant QTLs other than SUB1 through QTL mapping, newly identified submergence tolerant germplasm can be utilized.
{"title":"Identification of Novel Submergence Tolerant Local Rice Cultivars of Bangladesh","authors":"A. Amin, K. Iftekharuddaula, A. Sarker, A. Talukder, Sharmishtha Ghoshal, A. Shalahuddin, T. Aditya, Ansar Ali, B. Collard","doi":"10.11648/J.IJGG.20180604.12","DOIUrl":"https://doi.org/10.11648/J.IJGG.20180604.12","url":null,"abstract":"Bangladesh had harbored numerous submergence tolerant rice local cultivars or landraces (popularly known as Joli Aman). As the local cultivars endure intervallic flash-flooding year after year, it is presumed that genes/Quantitative Trait Loci (QTLs) that give resilience are probably going to be available in these genotypes. In submergence screening, the performance of three tested accessions (Lakhi, Atshotti and Damsi) demonstrated them to be as tolerant as the check FR13A. Other germplasm accessions (DSL-78-8, Laldepa, Putidepa, Laxmi digha) performed great recuperation capacity after de-submergence. Damsi, Rajasail, Haloi, Lal Digha, Manik Digha, Bhawalia, DSL-78-8, Putidepa, Atshotti and Jalda IRRI don't have the SUB1 QTL and furthermore not like the BR5, a submergence susceptible check variety. Bajal, Horkoach, Pathornuti, Laxmi Digha, Horinga Digha, Bhawalia Digha and BR5 was not amplified by Gns2 conferring SUB1 QTL (submergence tolerance specific QTL). Significantly, the local cultivars i.e. DSL-78-8, Putidepa and Sadadanga boro were identified as having better submergence tolerance however having no resistant alleles of GnS2 and Sub1C173, indel markers specific to SUB1 QTL. The result of molecular screening revealed new submergence tolerant rice cultivars which do not possess SUB1 QTL. To discover new submergence tolerant QTLs other than SUB1 through QTL mapping, newly identified submergence tolerant germplasm can be utilized.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"3 1","pages":"44"},"PeriodicalIF":0.0,"publicationDate":"2019-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88205971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-23DOI: 10.11648/j.ijgg.20180604.11
H. Abouzied, W. El-Orabey, M. Abou-Zeid
Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.
{"title":"Application of High Density Linkage Map Derived from Genotyping by Sequencing for Detection of QTL Conferring Resistance to Leaf Rust Races Spread in Egypt","authors":"H. Abouzied, W. El-Orabey, M. Abou-Zeid","doi":"10.11648/j.ijgg.20180604.11","DOIUrl":"https://doi.org/10.11648/j.ijgg.20180604.11","url":null,"abstract":"Wheat is a major source of carbohydrates in Egypt, leaf rust disease is known to be the most common rust disease affected wheat genotypes. This study aimed to apply a constructed high density linkage map through Genotyping-by- sequencing (GBS) for detection of QTL resistant to important leaf rust races spread in Egypt. The applied map contained 3,641 markers distributed on 21 chromosomes and spanned 1,959 cM with an average distance of 1.8 cM between markers. A mapping population of 204 RILs (F6:8) obtained from the cross between two parents of winter wheat ‘Harry’ x ‘Wesley’ through single seed decent method was used to identify QTL region associated with leaf rust resistance genes in wheat. Under the Green house condition in Egypt leaf rust pathotypes i.e NTTJT, PTTGS, PTTTT, TTTBT and TTTTT were used. High-density linkage map based on GBS derived SNPs were applied in this study for QTL mapping. The wheat genotype Harry was resistant to all tested pathotype while, the wheat genotype Wesley was susceptible to all tested pathotype. One major stable QTLs, for race NTTJT was identified on chromosome 6A flanked by markers XSNP3958 and XSNP3957 with a LOD 4.22. The identified SNP marker may be used to screen for resistance to specific races of leaf rust found in Egypt in further spring wheat breeding programs as marker assisted selection.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"68 1","pages":"37"},"PeriodicalIF":0.0,"publicationDate":"2019-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80686936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.35841/GENETICS-MOLECULAR-BIOLOGY.3.1-4
Anya Mi, A. Dauda, Ayuk Aa, Okon Bi, Okpako Op
Obudu Grass Plateau for phenotypic traits measurement. The phenotypic traits measured were Body Weight (BW), Body Length (BL), Height Withers (HW), Head Length (HDL), Head Width (HDW), Ear Length (EL), Horn Length (HL), Tail Length (TL), Dewlap width (DW), Scrotal Circumference (SC), Udder Circumference (UC), Number of Teats (NOT), F Fore Length (FLG), Hind Length (HLG), Cannon Circumference (CCF), Horn circumference (HNC), Cannon Length (CL), Rump Width (RW), Rump Length (RL), Heart Girth (HG). The data were analysed using SPSS. The fixed effects of age and lots on phenotypic traits were tested using linear model. The result revealed age and lots have significant (P<0.05) effect on phenotypic traits of cattle in Obudu cattle ranch. The result of correlation among phenotypic traits showed body weight has positive correlation rp (+) with all the phenotypic traits, while cannon length had negative rp (-) correlation with all the phenotypic traits except scrotal circumference (SC), udder circumference (UC) and body weight (BW). The positive correlation among phenotypic traits could be used for improvement because improvement in one will lead to improvement in others. The study will be useful in planning for selection and breeding programmes of cattle.
{"title":"Morphometric differentiation of mixed coat coloured cattle in Obudu Grass Plateau, South-South Nigeria.","authors":"Anya Mi, A. Dauda, Ayuk Aa, Okon Bi, Okpako Op","doi":"10.35841/GENETICS-MOLECULAR-BIOLOGY.3.1-4","DOIUrl":"https://doi.org/10.35841/GENETICS-MOLECULAR-BIOLOGY.3.1-4","url":null,"abstract":"Obudu Grass Plateau for phenotypic traits measurement. The phenotypic traits measured were Body Weight (BW), Body Length (BL), Height Withers (HW), Head Length (HDL), Head Width (HDW), Ear Length (EL), Horn Length (HL), Tail Length (TL), Dewlap width (DW), Scrotal Circumference (SC), Udder Circumference (UC), Number of Teats (NOT), F Fore Length (FLG), Hind Length (HLG), Cannon Circumference (CCF), Horn circumference (HNC), Cannon Length (CL), Rump Width (RW), Rump Length (RL), Heart Girth (HG). The data were analysed using SPSS. The fixed effects of age and lots on phenotypic traits were tested using linear model. The result revealed age and lots have significant (P<0.05) effect on phenotypic traits of cattle in Obudu cattle ranch. The result of correlation among phenotypic traits showed body weight has positive correlation rp (+) with all the phenotypic traits, while cannon length had negative rp (-) correlation with all the phenotypic traits except scrotal circumference (SC), udder circumference (UC) and body weight (BW). The positive correlation among phenotypic traits could be used for improvement because improvement in one will lead to improvement in others. The study will be useful in planning for selection and breeding programmes of cattle.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89526221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-06DOI: 10.11648/J.IJGG.20180603.11
S. Ba, F. Mbaye, Matar Ciss, N. Guéye, M. Sembéne
Cataract and glaucoma are the leading causes of blindness in the world. The aim of this study was to investigate the involvement of somatic mutations of the MT-CYB gene in glaucoma and cataract cases among Senegalese patients. 38 samples including 12 controls and 26 patients (18 cataracts, 8 glaucomas) were used to study polymorphism, diversity and genetic structuration of the MT-CYB gene. DNA was isolated from whole blood samples and then the gene was genotyped by PCR-Sequencing. The study of the chromatograms and sequences obtained made it possible to detect a total of twenty-four (24) variants among which nine (9) non-synonymous mutations with seven (7) different positions. Two of them were common to both pathologies (G71A, T96C) and predicted to be non-pathogenic. The insertion, T235TA was specific for glaucoma and is known to be pathogenic. Four (4) mutations were specific to cataract (T118TC, A401AC, G402C and A408AC). T118TC and A408C are predicted pathogenic. A total of four (4) haplogroups (H, J, L and M) were found in this study. The haplogroups H and L are significantly represented in patients with cataract (H: p-value = 1.72182e-09 and L: p-value = 0.000351) and glaucoma (H: p-value = 3.333e-08 and L: p-value = 0.009398). The results also revealed a differentiation only between controls and glaucoma patients (Fst = 0.17144 and p-value = 0.0019) and between controls and glaucomatous belonging to haplogroup L (Fst = 0.47368 and p-value = 0.0156). The conclusions from this work were that contrary to cataract, MT-CYB somatic mutations are involved in the occurrence of glaucoma in Senegalese and this involvement is correlated with mitochondrial haplogroup L.
{"title":"Mitochondrial DNA Nucleotide Changes in Cataract and Glaucoma Patients in Senegal","authors":"S. Ba, F. Mbaye, Matar Ciss, N. Guéye, M. Sembéne","doi":"10.11648/J.IJGG.20180603.11","DOIUrl":"https://doi.org/10.11648/J.IJGG.20180603.11","url":null,"abstract":"Cataract and glaucoma are the leading causes of blindness in the world. The aim of this study was to investigate the involvement of somatic mutations of the MT-CYB gene in glaucoma and cataract cases among Senegalese patients. 38 samples including 12 controls and 26 patients (18 cataracts, 8 glaucomas) were used to study polymorphism, diversity and genetic structuration of the MT-CYB gene. DNA was isolated from whole blood samples and then the gene was genotyped by PCR-Sequencing. The study of the chromatograms and sequences obtained made it possible to detect a total of twenty-four (24) variants among which nine (9) non-synonymous mutations with seven (7) different positions. Two of them were common to both pathologies (G71A, T96C) and predicted to be non-pathogenic. The insertion, T235TA was specific for glaucoma and is known to be pathogenic. Four (4) mutations were specific to cataract (T118TC, A401AC, G402C and A408AC). T118TC and A408C are predicted pathogenic. A total of four (4) haplogroups (H, J, L and M) were found in this study. The haplogroups H and L are significantly represented in patients with cataract (H: p-value = 1.72182e-09 and L: p-value = 0.000351) and glaucoma (H: p-value = 3.333e-08 and L: p-value = 0.009398). The results also revealed a differentiation only between controls and glaucoma patients (Fst = 0.17144 and p-value = 0.0019) and between controls and glaucomatous belonging to haplogroup L (Fst = 0.47368 and p-value = 0.0156). The conclusions from this work were that contrary to cataract, MT-CYB somatic mutations are involved in the occurrence of glaucoma in Senegalese and this involvement is correlated with mitochondrial haplogroup L.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"26 1","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2018-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74097167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pancasakti Kusumaningrum, Hermin, B. Anto, Suprihadi Agung, Eshananda Yuriza, Fadillah Annisa, Rahayuning Pangestuti Dina
{"title":"The characterization of Citrus sp. from Parang Island Karimunjawa based on morphological, DNA barcoding and nutritional analysis","authors":"Pancasakti Kusumaningrum, Hermin, B. Anto, Suprihadi Agung, Eshananda Yuriza, Fadillah Annisa, Rahayuning Pangestuti Dina","doi":"10.5897/ijgmb2018.0167","DOIUrl":"https://doi.org/10.5897/ijgmb2018.0167","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87505536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-30DOI: 10.9735/0975-2862.10.4.415-419
B. R. Beniwal, E. Sastry, Z. Solanki
{"title":"Combining Ability And Heterosis Studies In Sesame (Sesamum indicum L)","authors":"B. R. Beniwal, E. Sastry, Z. Solanki","doi":"10.9735/0975-2862.10.4.415-419","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.415-419","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88461348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-30DOI: 10.9735/0975-2862.10.4.412-414
Balwan Raliya, R. Jat, H. Meena, R. Mundiyara, K. Kumar, Ramesh, Pukhraj
{"title":"Genetic Studies For Seed Yield And Component Traits In Mustard (Brassica Juncea L)","authors":"Balwan Raliya, R. Jat, H. Meena, R. Mundiyara, K. Kumar, Ramesh, Pukhraj","doi":"10.9735/0975-2862.10.4.412-414","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.412-414","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87164881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-30DOI: 10.9735/0975-2862.10.4.434-437
Y. Vivekananda, Jesmi Devi Warepam, J. Ahongshangbam, N. Singh, J. M. Laishram
{"title":"Identification Of Maize Line Of Manipur Containing crtRB1 3’te Favourable Allele Using Molecular Marker","authors":"Y. Vivekananda, Jesmi Devi Warepam, J. Ahongshangbam, N. Singh, J. M. Laishram","doi":"10.9735/0975-2862.10.4.434-437","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.434-437","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78555160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-30DOI: 10.9735/0975-2862.10.4.425-433
G. S. Sanghera, Lenika Kashyap, Anuradha, Rajinder Kumar, R. Bhatt, Harmandeep Singh
{"title":"Genetic Variability Studies In Sugarcane (Saccharum Sp. Hybrid Complex) Under Water Stress And Non-stress Conditions","authors":"G. S. Sanghera, Lenika Kashyap, Anuradha, Rajinder Kumar, R. Bhatt, Harmandeep Singh","doi":"10.9735/0975-2862.10.4.425-433","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.425-433","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"135 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89749279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-30DOI: 10.9735/0975-2862.10.4.420-424
G. Lokesh, Garima Madhariya, A. Srivastava, P. Kar, P. Srivastava, A. Sinha, A. Sahay
{"title":"Impact of High Temperature Gradients on the Physiolgical and Biomolecular Changes in the Tropical Tasar Silkworm Antheraea mylitta D","authors":"G. Lokesh, Garima Madhariya, A. Srivastava, P. Kar, P. Srivastava, A. Sinha, A. Sahay","doi":"10.9735/0975-2862.10.4.420-424","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.420-424","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82320954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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