Pub Date : 2018-05-22DOI: 10.11648/j.ijgg.20180602.11
Sognan Dao, Halimatou Timbine, O. Goita, D. Traoré
Rice species (Oryza sativa, L. and Oryza glaberrima, Steud) provide 20% energy of world’s food. Knowledge of genetic variability is important to remove duplicate materials for gene bank management and conservation. In this study, 59 microsatellite markers were used to assess the genetic diversity of 54 intraspecific (Oryza sativa) and interspecific (Oryza sativa X Oryza glaberrima) irrigated rice varieties by PCR-SSR. A total of 250 alleles were detected with an average of 4.24 alleles per SSR. Genetic diversity was ranged from 0.0713 (RM333, RM3744) to 0.8937 (RM251) to with an average of 0.4325. Polymorphism Information Content (PIC) varied from 0.0688 (RM333, RM3744) to 0.8854 (RM251) with an average of 0.3940. Rice genotypes were shared between five Groups based on their similarity with majority of them (64.81%) under Group V. Variety SK 7-8 within Group III was highly dissimilar to other varieties. Malian varieties were shared out between Groups II, III, VI and V. Strong genetic variability was observed within cluster V2 of Group V among the most cultivated and appreciated rice varieties such as Kogoni 91-1, Adny11 and BG90-2. Allelic variability observed among rice varieties could help breeding programs to identify cultivars with good agronomic traits for crop improvement.
{"title":"Genetic Variability Assessment in Irrigated Rice ( Oryza sativa and Oryza glaberrima ) by PCR-SSR in Mali","authors":"Sognan Dao, Halimatou Timbine, O. Goita, D. Traoré","doi":"10.11648/j.ijgg.20180602.11","DOIUrl":"https://doi.org/10.11648/j.ijgg.20180602.11","url":null,"abstract":"Rice species (Oryza sativa, L. and Oryza glaberrima, Steud) provide 20% energy of world’s food. Knowledge of genetic variability is important to remove duplicate materials for gene bank management and conservation. In this study, 59 microsatellite markers were used to assess the genetic diversity of 54 intraspecific (Oryza sativa) and interspecific (Oryza sativa X Oryza glaberrima) irrigated rice varieties by PCR-SSR. A total of 250 alleles were detected with an average of 4.24 alleles per SSR. Genetic diversity was ranged from 0.0713 (RM333, RM3744) to 0.8937 (RM251) to with an average of 0.4325. Polymorphism Information Content (PIC) varied from 0.0688 (RM333, RM3744) to 0.8854 (RM251) with an average of 0.3940. Rice genotypes were shared between five Groups based on their similarity with majority of them (64.81%) under Group V. Variety SK 7-8 within Group III was highly dissimilar to other varieties. Malian varieties were shared out between Groups II, III, VI and V. Strong genetic variability was observed within cluster V2 of Group V among the most cultivated and appreciated rice varieties such as Kogoni 91-1, Adny11 and BG90-2. Allelic variability observed among rice varieties could help breeding programs to identify cultivars with good agronomic traits for crop improvement.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"1 1","pages":"22"},"PeriodicalIF":0.0,"publicationDate":"2018-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79942833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-05DOI: 10.11648/J.IJGG.20180601.13
L. M. A. Neghery, R. Kenana, Albandary Al Bakheet, Rawan Al Mass, Faten Al Mutairi, Maysoon Al Sagob, A. Qari, Rozeena Huma, D. Çolak, M. Daghestani, N. Kaya, Moeen Al‐ Sayed
Mutations in ADP-ribosylation factor guanine nucleotide-exchange factor 2 (ARFGEF2) lead to autosomal recessive periventricular heterotopia (PH). To date, 11 mutations, (six missense mutations, one splicing mutation, one small deletion, two small insertions, and one small deletion/insertion) have been reported. Assessing ARFGEF2 mutations will provide a holistic overview of PH. This retrospective study was conducted in 2016 at King Faisal Specialist Hospital and Research Center. For the index patient, magnetic resonance imaging studies revealed a symmetrical focal hyperintensity involving the putamen bilaterally and the inner aspect of the globus pallidus. After family members were genotyped, an autozygosity analysis was performed, followed by exome sequencing of the index patient; A comprehensive filtering approach based on the loss of heterozygosity (LOH) was used to identify variants in phenotypically relevant genes. We report a consanguineous family with two affected individuals, a boy and a girl, with a history of microcephaly, global developmental delay, intellectual disability, myoclonic seizure, and dystonia. The patients carried a novel nonsense mutation (c.3974G>A, p. Trp1325*) in the Armadillo-type fold domain of ARFGEF2. These findings extend our understanding of the phenotype–genotype correlations for ARFGEF2 mutations.
adp核糖基化因子鸟嘌呤核苷酸交换因子2 (ARFGEF2)突变导致常染色体隐性心室周围异位(PH)。迄今为止，已报道了11个突变(6个错义突变，1个剪接突变，1个小缺失，2个小插入和1个小缺失/插入)。评估ARFGEF2突变将提供ph的整体概况。这项回顾性研究于2016年在费萨尔国王专科医院和研究中心进行。对于指数患者，磁共振成像研究显示对称的局灶性高强度涉及双侧壳核和苍白球内侧。在对家庭成员进行基因分型后，进行自合子分析，然后对索引患者进行外显子组测序;基于杂合性损失(LOH)的综合过滤方法被用于鉴定表型相关基因的变异。我们报告了一个近亲家庭，有两个患病个体，一男一女，有小头畸形、整体发育迟缓、智力残疾、肌阵挛性发作和肌张力障碍的病史。患者在ARFGEF2的Armadillo-type fold domain中携带一个新的无义突变(c.3974G> a, p. Trp1325*)。这些发现扩展了我们对ARFGEF2突变的表型-基因型相关性的理解。
{"title":"A Systematic Genetic Assessment of ARFGEF2 Mutations in Periventricular Heterotopia","authors":"L. M. A. Neghery, R. Kenana, Albandary Al Bakheet, Rawan Al Mass, Faten Al Mutairi, Maysoon Al Sagob, A. Qari, Rozeena Huma, D. Çolak, M. Daghestani, N. Kaya, Moeen Al‐ Sayed","doi":"10.11648/J.IJGG.20180601.13","DOIUrl":"https://doi.org/10.11648/J.IJGG.20180601.13","url":null,"abstract":"Mutations in ADP-ribosylation factor guanine nucleotide-exchange factor 2 (ARFGEF2) lead to autosomal recessive periventricular heterotopia (PH). To date, 11 mutations, (six missense mutations, one splicing mutation, one small deletion, two small insertions, and one small deletion/insertion) have been reported. Assessing ARFGEF2 mutations will provide a holistic overview of PH. This retrospective study was conducted in 2016 at King Faisal Specialist Hospital and Research Center. For the index patient, magnetic resonance imaging studies revealed a symmetrical focal hyperintensity involving the putamen bilaterally and the inner aspect of the globus pallidus. After family members were genotyped, an autozygosity analysis was performed, followed by exome sequencing of the index patient; A comprehensive filtering approach based on the loss of heterozygosity (LOH) was used to identify variants in phenotypically relevant genes. We report a consanguineous family with two affected individuals, a boy and a girl, with a history of microcephaly, global developmental delay, intellectual disability, myoclonic seizure, and dystonia. The patients carried a novel nonsense mutation (c.3974G>A, p. Trp1325*) in the Armadillo-type fold domain of ARFGEF2. These findings extend our understanding of the phenotype–genotype correlations for ARFGEF2 mutations.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"51 1","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2018-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86644387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-05-05DOI: 10.11648/j.ijgg.20180601.14
Jesus David Sendoya Vargas, Divya Periasamy, F. Sánchez, Maria Alejandra Benavides Fierro, Ingrid Carolina Duran Palacios, R. Sidlow, Henry Ostos Alfonso
Duplications of the long arm of chromosome 3 most frequently occur in the context of other chromosomal copy number variations. Pure duplications of this region are exceedingly rare, varying widely in size and clinical presentation. Presented below is a case of a small pure duplication of the long arm of chromosome 3q25.1 associated with marked cerebral organization defects and the possible genotype/phenotype correlation between the two.
{"title":"A Small Pure 3q25.1 Duplication Associated with Multiple Cerebral Organizational Defects","authors":"Jesus David Sendoya Vargas, Divya Periasamy, F. Sánchez, Maria Alejandra Benavides Fierro, Ingrid Carolina Duran Palacios, R. Sidlow, Henry Ostos Alfonso","doi":"10.11648/j.ijgg.20180601.14","DOIUrl":"https://doi.org/10.11648/j.ijgg.20180601.14","url":null,"abstract":"Duplications of the long arm of chromosome 3 most frequently occur in the context of other chromosomal copy number variations. Pure duplications of this region are exceedingly rare, varying widely in size and clinical presentation. Presented below is a case of a small pure duplication of the long arm of chromosome 3q25.1 associated with marked cerebral organization defects and the possible genotype/phenotype correlation between the two.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"47 1","pages":"18"},"PeriodicalIF":0.0,"publicationDate":"2018-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90962919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-30DOI: 10.9735/0975-2862.10.4.390-393
A. Sharma, M. Sarma, N. Ramchiary
{"title":"GENETIC VARIABILITY AND CHARACTER ASSOCIATION IN SOYBEAN GERMPLASM FOR PRE-HARVEST SPROUTING TOLERANCE AND ASSOCIATED TRAITS","authors":"A. Sharma, M. Sarma, N. Ramchiary","doi":"10.9735/0975-2862.10.4.390-393","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.390-393","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"12 4","pages":"390"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72452559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Quain, K. Adofo, D. Appiah-Kubi, R. Prempeh, J. Asafu-Agyei, Belinda Akomeah, Harrison Kwame Dapaah
Development of improved varieties of sweet potato to meet specific end users in the sub-Saharan African region has the potential of transforming the crop’s market, consumption and health benefits. Molecular biology techniques such as fingerprinting are key to crop improvement, and critical for numerous genetic studies. This study, carried out in Ghana, seeks to determine genetic relatedness among two populations of sweet potato (released and elite), as well as to identify polymorphic and informative markers for diversity studies. Twenty expressed sequence tags-simple sequence repeat (EST-SSR) markers were used for analysis of DNA assembled. A total of 169 alleles were obtained, with an average of 8.5 alleles per locus. Pairwise genetic distance among genotypes ranged from 12 – 89. GenAlex v6.4 analysis showed that a higher genetic diversity existed within elite genotypes than the released lines. Results analyzed using Popgene version 1.31 software indicated that on the basis of both populations studied, EST-SSR markers utilized were very polymorphic (95%) and showed genetic diversity (I =0.888, He= 0.548) suggesting high heterozygosity in the collected varieties. The overall Fit (0.372%) and Fis (0.088%) suggest high genetic diversity within populations, and gene flow (1.95) indicate an exchange of genes between released sweet potatoes and elite ones. Ewens-Watterson test was conducted to test the neutrality. From the results obtained, markers CB329940, CB330477 and CB330936 showed high allelic polymorphism among varieties used and were recommended to be included in diversity studies. Key words: Genetic diversity, genetic relatedness, expressed sequence tags-simple sequence repeat (EST-SSR), populations, released sweet potato, elite sweet potato.
{"title":"Use of expressed sequence tags-derived simple sequence repeat (SSR) markers for population studies of released and elite sweet potato","authors":"M. Quain, K. Adofo, D. Appiah-Kubi, R. Prempeh, J. Asafu-Agyei, Belinda Akomeah, Harrison Kwame Dapaah","doi":"10.5897/IJGMB2017.0159","DOIUrl":"https://doi.org/10.5897/IJGMB2017.0159","url":null,"abstract":"Development of improved varieties of sweet potato to meet specific end users in the sub-Saharan African region has the potential of transforming the crop’s market, consumption and health benefits. Molecular biology techniques such as fingerprinting are key to crop improvement, and critical for numerous genetic studies. This study, carried out in Ghana, seeks to determine genetic relatedness among two populations of sweet potato (released and elite), as well as to identify polymorphic and informative markers for diversity studies. Twenty expressed sequence tags-simple sequence repeat (EST-SSR) markers were used for analysis of DNA assembled. A total of 169 alleles were obtained, with an average of 8.5 alleles per locus. Pairwise genetic distance among genotypes ranged from 12 – 89. GenAlex v6.4 analysis showed that a higher genetic diversity existed within elite genotypes than the released lines. Results analyzed using Popgene version 1.31 software indicated that on the basis of both populations studied, EST-SSR markers utilized were very polymorphic (95%) and showed genetic diversity (I =0.888, He= 0.548) suggesting high heterozygosity in the collected varieties. The overall Fit (0.372%) and Fis (0.088%) suggest high genetic diversity within populations, and gene flow (1.95) indicate an exchange of genes between released sweet potatoes and elite ones. Ewens-Watterson test was conducted to test the neutrality. From the results obtained, markers CB329940, CB330477 and CB330936 showed high allelic polymorphism among varieties used and were recommended to be included in diversity studies. \u0000 \u0000 \u0000 \u0000 Key words: Genetic diversity, genetic relatedness, expressed sequence tags-simple sequence repeat (EST-SSR), populations, released sweet potato, elite sweet potato.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"26 1","pages":"14-25"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75533533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-30DOI: 10.9735/0975-2862.10.4.387-389
Deepika, G. Sharma
{"title":"PERFORMANCE OF STRAWBERRY F1 HYBRIDS AND PARENTS FOR FLOWERING, FRUITING AND VEGETATIVE PARAMETERS","authors":"Deepika, G. Sharma","doi":"10.9735/0975-2862.10.4.387-389","DOIUrl":"https://doi.org/10.9735/0975-2862.10.4.387-389","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"16 1","pages":"387"},"PeriodicalIF":0.0,"publicationDate":"2018-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88555393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-15DOI: 10.9735/0975-2862.10.3.365-369
A. Nath, S. Maloo, Devendra Jain, S. Nath, A. Chakma, R. Verma
Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied method is marker analysis. RAPD and ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes. Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement. The RAPD and ISSR data were evaluated to obtain a combined similarity matrix. The similarity coefficient values lay between 0.46–0.68. The RAPD and ISSR cluster tree analysis showed that the eight genotypes could be divided into 4 clusters. The genotype BM 4 was grouped in separate VI cluster. However, PDM 139 was grouped on cluster IIA. In the light of RAPD and ISSR study the parents of the cross BM 4 x PDM 139 were also noticed for their genetic diversity, having 53% dissimilarity and grouped into the separate clusters.
{"title":"Genetic Confirmation of Mungbean Genotypes (Vigna Radiata L. Wilczek) Using Molecular Markers","authors":"A. Nath, S. Maloo, Devendra Jain, S. Nath, A. Chakma, R. Verma","doi":"10.9735/0975-2862.10.3.365-369","DOIUrl":"https://doi.org/10.9735/0975-2862.10.3.365-369","url":null,"abstract":"Molecular characterization is helpful in understanding the phylogenetic relationship among various germplasm to reveal the genetic diversity among the used parental genotypes. Among several efficient methods for revealing genetic variability within and among plant populations, one of the most widely applied method is marker analysis. RAPD and ISSR, markers are commonly used because they are quick, simple and environment non-sensitive enabling genetic diversity analysis in several types of plant material like natural populations, population in breeding programmes. Evaluation of genetic diversity would promote the efficient use of genetic variations, effective conservation and purity of the genotype to be determined as well as utilization of germplasm in crop improvement. The RAPD and ISSR data were evaluated to obtain a combined similarity matrix. The similarity coefficient values lay between 0.46–0.68. The RAPD and ISSR cluster tree analysis showed that the eight genotypes could be divided into 4 clusters. The genotype BM 4 was grouped in separate VI cluster. However, PDM 139 was grouped on cluster IIA. In the light of RAPD and ISSR study the parents of the cross BM 4 x PDM 139 were also noticed for their genetic diversity, having 53% dissimilarity and grouped into the separate clusters.","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"5 1","pages":"365"},"PeriodicalIF":0.0,"publicationDate":"2018-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81408059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-15DOI: 10.9735/0975-2862.10.3.373-375
T. Bhutia, A. Munshi, A. K. Sureja, B. Gurung, S. Singh
{"title":"Estimates of Genetic Component of Variances and Genetic Ratios for Earliness and Yield Attributing Traits in Cucumber","authors":"T. Bhutia, A. Munshi, A. K. Sureja, B. Gurung, S. Singh","doi":"10.9735/0975-2862.10.3.373-375","DOIUrl":"https://doi.org/10.9735/0975-2862.10.3.373-375","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"3 1","pages":"373"},"PeriodicalIF":0.0,"publicationDate":"2018-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84452477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-15DOI: 10.9735/0975-2862.10.3.376-379
Jyoti Singh, Shubha Banerjee
{"title":"Phylogenetic Relationship among the Wild Rice (Oryza Nivara) and Intraspecies of Rice Genotypes Belonging to Chhattisgarh Revealed by Chloroplast Dna Regions psbA-trnH","authors":"Jyoti Singh, Shubha Banerjee","doi":"10.9735/0975-2862.10.3.376-379","DOIUrl":"https://doi.org/10.9735/0975-2862.10.3.376-379","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"22 1","pages":"376"},"PeriodicalIF":0.0,"publicationDate":"2018-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78238201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-04-15DOI: 10.9735/0975-2862.10.3.370-372
Suchit Kumar, A. Mukherjee, Sunil Kumar, A. Yadav, N. Kumar
{"title":"Role of Osteopontin in Bovines and their Association with Different Traits: A Review","authors":"Suchit Kumar, A. Mukherjee, Sunil Kumar, A. Yadav, N. Kumar","doi":"10.9735/0975-2862.10.3.370-372","DOIUrl":"https://doi.org/10.9735/0975-2862.10.3.370-372","url":null,"abstract":"","PeriodicalId":88902,"journal":{"name":"International journal of genetics and molecular biology","volume":"83 1","pages":"370"},"PeriodicalIF":0.0,"publicationDate":"2018-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75305931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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