Pub Date : 2019-01-21DOI: 10.11648/j.plm.20190301.12
Huiping Liang, T. Lu, Huaying Liu, L. Tan, Li Li, Xiao-Jing Tang
Background: Although there are many biochemical indicators to reflect changes in renal function, but these indicators can not reflect renal damage timely and accurately. Renal microcirculation changes can not be reflected also. This study amied at evaluate significance of renal perfusion angiography and the indexes of relevant biochemical in early diagnosis of type 2 diabetic nephropathy. Methods: 30 I-III T2DN patients (DN group) and 25 healthy volunteers (control group) were studied, and both of groups were performed renal perfusion CT angiography imaging examination. Biochemical indexes, which are fasting blood glucose (FBG), urinary albumin excretion rate (UAE), 24 h urine protein quantitation (UPQ), blood urea nitrogen (BUN), serum creatinine (Scr), Cystatin C(Cys C), random albumin to creatinine ratio(ACR), were measured in both of groups, then simplified MDRD (Modification of Diet in Renal Disease) and Cys C equation were used to calculate the Estimated Glomerular Filtration Rate(eGFR). double-renal perfusion contrast scanning in both of groups were also conducted to obtain data of double renal blood flow (BF), blood volume (BV), mean transit time (MTT), and permeability surface (PS) for statistical analysis. Results: The indexes of BF, MTT and FBG, UAE, 24 h UPQ, ACR, Cys C, eGFR were found to have relative strong correlation and statistical significance (P<0.05). BF was shown the negative correlation with those indexes, however MTT had positive correlation with them. The area under ROC (receiver operating characteristic curve), were more than 0.9 for UAE, ACR, NF, and MTT, and it indicate that these indexes have relatively positive effect on the early diagnosis of the DN. For 24 h UPQ, cysteine C, eGFR and BV value, the area Under the curve are between 0.7 and 0.9, which indicates that diagnosis using those index for the disease were accuracy. Thus, the above indexes have better diagnostic efficiency compared to Scr, BUN and eGFR. Conclusion: The biochemical indexes such as UAE, ACR and renal perfusion indexes such as BF and MTT can be applied in the early predicative diagnosis and DN screening. Its diagnosis effect is better than other biochemical indexes.
背景:虽然有许多生化指标可以反映肾功能的变化，但这些指标并不能及时、准确地反映肾脏损害。肾脏微循环变化也不能反映。本研究旨在评价肾灌注血管造影及相关生化指标在2型糖尿病肾病早期诊断中的意义。方法:选取30例I-III型T2DN患者(DN组)和25例健康志愿者(对照组)，两组均行肾灌注CT血管造影成像检查。测定两组患者空腹血糖(FBG)、尿白蛋白排泄率(UAE)、24 h尿蛋白定量(UPQ)、血尿素氮(BUN)、血清肌酐(Scr)、胱抑素C(Cys C)、随机白蛋白/肌酐比(ACR)等生化指标，采用简化的MDRD (dietary Modification in Renal Disease)和Cys C方程计算肾小球滤过率(Estimated Glomerular Filtration rate, eGFR)。两组患者均行双肾灌注造影扫描，获取双肾血流量(BF)、血容量(BV)、平均传递时间(MTT)、通透面(PS)等数据进行统计分析。结果:BF、MTT、FBG、UAE、24 h UPQ、ACR、Cys C、eGFR等指标相关性较强，差异均有统计学意义(P<0.05)。BF与这些指标呈负相关，而MTT与这些指标呈正相关。UAE、ACR、NF、MTT的受试者工作特征曲线下面积均大于0.9，说明这些指标对DN的早期诊断有相对积极的作用。对于24 h UPQ、半胱氨酸C、eGFR和BV值，曲线下面积在0.7 ~ 0.9之间，说明使用这些指标对疾病的诊断是准确的。因此，上述指标较Scr、BUN、eGFR具有更好的诊断效能。结论:UAE、ACR等生化指标和BF、MTT等肾灌注指标可用于早期预测诊断和DN筛查。其诊断效果优于其他生化指标。
{"title":"Significance of Renal Perfusion Angiography and Biochemical Indicators in Early Diagnosis of Type 2 Diabetic Nephropathy","authors":"Huiping Liang, T. Lu, Huaying Liu, L. Tan, Li Li, Xiao-Jing Tang","doi":"10.11648/j.plm.20190301.12","DOIUrl":"https://doi.org/10.11648/j.plm.20190301.12","url":null,"abstract":"Background: Although there are many biochemical indicators to reflect changes in renal function, but these indicators can not reflect renal damage timely and accurately. Renal microcirculation changes can not be reflected also. This study amied at evaluate significance of renal perfusion angiography and the indexes of relevant biochemical in early diagnosis of type 2 diabetic nephropathy. Methods: 30 I-III T2DN patients (DN group) and 25 healthy volunteers (control group) were studied, and both of groups were performed renal perfusion CT angiography imaging examination. Biochemical indexes, which are fasting blood glucose (FBG), urinary albumin excretion rate (UAE), 24 h urine protein quantitation (UPQ), blood urea nitrogen (BUN), serum creatinine (Scr), Cystatin C(Cys C), random albumin to creatinine ratio(ACR), were measured in both of groups, then simplified MDRD (Modification of Diet in Renal Disease) and Cys C equation were used to calculate the Estimated Glomerular Filtration Rate(eGFR). double-renal perfusion contrast scanning in both of groups were also conducted to obtain data of double renal blood flow (BF), blood volume (BV), mean transit time (MTT), and permeability surface (PS) for statistical analysis. Results: The indexes of BF, MTT and FBG, UAE, 24 h UPQ, ACR, Cys C, eGFR were found to have relative strong correlation and statistical significance (P<0.05). BF was shown the negative correlation with those indexes, however MTT had positive correlation with them. The area under ROC (receiver operating characteristic curve), were more than 0.9 for UAE, ACR, NF, and MTT, and it indicate that these indexes have relatively positive effect on the early diagnosis of the DN. For 24 h UPQ, cysteine C, eGFR and BV value, the area Under the curve are between 0.7 and 0.9, which indicates that diagnosis using those index for the disease were accuracy. Thus, the above indexes have better diagnostic efficiency compared to Scr, BUN and eGFR. Conclusion: The biochemical indexes such as UAE, ACR and renal perfusion indexes such as BF and MTT can be applied in the early predicative diagnosis and DN screening. Its diagnosis effect is better than other biochemical indexes.","PeriodicalId":88950,"journal":{"name":"Pathology and laboratory medicine international","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74556438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zinc-binding protein-89 (ZBP-89; ZNF148, BERF-1, BFCOL-1) is a zinc-finger transcription factor of the Kruppel family. It has been shown to regulate the expression of a number of genes, acting as either an activator or repressor of gene expression, depending on the context. It is over-expressed in several cancers, but has been shown to be involved in apoptosis and to have a negative influence on cell growth in part by interactions with p53. Previously, ZBP-89 was shown to activate transcription of the matrix metalloproteinase-3 (MMP-3) gene by binding to a polymorphic promoter element in competition with nuclear factor kappaB (NF-kappaB). NF-kappaB is known to be a key regulator of the inflammatory response, but relatively little is known about regulation of ZBP-89. In order to ascertain whether ZBP-89 is regulated during inflammation, we designed experiments to determine whether and to what extent ZBP-89 levels are affected by inflammatory cytokines. Here we show that ZBP-89 mRNA and protein expression are significantly inhibited in human fibroblasts by the inflammatory cytokine interleukin-1beta. Since any change in the levels of ZBP-89 would presumably impact the regulation of MMP-3 and other ZBP-89 target genes, these results provide important insight into mechanisms involved in fine-tuning the immune response.
{"title":"Expression of transcription factor zinc-binding protein-89 (ZBP-89) is inhibited by inflammatory cytokines.","authors":"Ruth C Borghaei, Mariah Chambers","doi":"10.2147/plmi.s6249","DOIUrl":"10.2147/plmi.s6249","url":null,"abstract":"<p><p>Zinc-binding protein-89 (ZBP-89; ZNF148, BERF-1, BFCOL-1) is a zinc-finger transcription factor of the Kruppel family. It has been shown to regulate the expression of a number of genes, acting as either an activator or repressor of gene expression, depending on the context. It is over-expressed in several cancers, but has been shown to be involved in apoptosis and to have a negative influence on cell growth in part by interactions with p53. Previously, ZBP-89 was shown to activate transcription of the matrix metalloproteinase-3 (MMP-3) gene by binding to a polymorphic promoter element in competition with nuclear factor kappaB (NF-kappaB). NF-kappaB is known to be a key regulator of the inflammatory response, but relatively little is known about regulation of ZBP-89. In order to ascertain whether ZBP-89 is regulated during inflammation, we designed experiments to determine whether and to what extent ZBP-89 levels are affected by inflammatory cytokines. Here we show that ZBP-89 mRNA and protein expression are significantly inhibited in human fibroblasts by the inflammatory cytokine interleukin-1beta. Since any change in the levels of ZBP-89 would presumably impact the regulation of MMP-3 and other ZBP-89 target genes, these results provide important insight into mechanisms involved in fine-tuning the immune response.</p>","PeriodicalId":88950,"journal":{"name":"Pathology and laboratory medicine international","volume":"1 ","pages":"7-12"},"PeriodicalIF":0.8,"publicationDate":"2009-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913887/pdf/nihms171948.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29171087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2003-01-01DOI: 10.1007/978-1-59259-385-9
Alan H. B. Wu, John R. Petersen, J. A. Lott, William E. Boden
{"title":"Cardiac Markers","authors":"Alan H. B. Wu, John R. Petersen, J. A. Lott, William E. Boden","doi":"10.1007/978-1-59259-385-9","DOIUrl":"https://doi.org/10.1007/978-1-59259-385-9","url":null,"abstract":"","PeriodicalId":88950,"journal":{"name":"Pathology and laboratory medicine international","volume":"331 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77127256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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