Pub Date : 2021-08-23DOI: 10.31258/biogenesis.17.2.81-87
Viola Vinca Valisa, E. Suryawati, Arnentis Arnentis
The most problem occurs in the praticum in the real laboratory, so it's not going well. It can affect the depth of concepts, principles, laws and theories that must be understood by the biology teacher candidates. One of the alternatives that can be done is practicum to use a virtual laboratory. The purpose of research is to develop a practical guidebook for animal tissue materials in practicum activities using virtual laboratories for teacher candidates. This research was conducted at the Laboratory of PMIPA FKIP Riau University in December 2020–May 2021. It used a Research and Development (R&D) approach with ADDIE model. The instruments used are validation expert and response questionnaires. The results showed that the guide books is good quality and suitable to be used as independent learning resources to increase content knowledge for teacher candidates.
{"title":"BUKU PENUNTUN PRAKTIKUM JARINGAN HEWAN MENGGUNAKAN VIRTUAL LABORATORY","authors":"Viola Vinca Valisa, E. Suryawati, Arnentis Arnentis","doi":"10.31258/biogenesis.17.2.81-87","DOIUrl":"https://doi.org/10.31258/biogenesis.17.2.81-87","url":null,"abstract":"The most problem occurs in the praticum in the real laboratory, so it's not going well. It can affect the depth of concepts, principles, laws and theories that must be understood by the biology teacher candidates. One of the alternatives that can be done is practicum to use a virtual laboratory. The purpose of research is to develop a practical guidebook for animal tissue materials in practicum activities using virtual laboratories for teacher candidates. This research was conducted at the Laboratory of PMIPA FKIP Riau University in December 2020–May 2021. It used a Research and Development (R&D) approach with ADDIE model. The instruments used are validation expert and response questionnaires. The results showed that the guide books is good quality and suitable to be used as independent learning resources to increase content knowledge for teacher candidates.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75843815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-23DOI: 10.31258/biogenesis.17.2.69-80
Hafizah Saleh, Binari Manurung, Ely Djulia
This development research aims to determine: (1) Content Feasibility; (2) Feasibility of Presentation, (3) Feasibility of Language (Readability), and (4) Feasibility of Design of Ceria Cartoon Calendar (Indonesian Youth Story) for Junior High School students. The research procedure uses the Thiagarajan development model which consists of four stages (four-D models), namely: define stage, design stage, develop stage and disseminate. The feasibility analysis was carried out using a questionnaire instrument which was assessed by three parties consisting of Expert Validators, Biology Teacher Reviewers, and a Student Perception Questionnaire of the Cheerful Cartoon Calendar (Indonesian Youth Story) Biology Class IX SMP PGRI 24 Medan. Analysis. The results of this study showed that: (1) The Feasibility of the Contents of the Ceria Cartoon Calendar (Indonesian Youth Story) obtained good criteria, (2) the Feasibility of the Presentation of the Cheerful Cartoon Calendar (Indonesian Youth Story) obtained very good criteria, (3) the Feasibility of Language (Readability) Calendar Ceria Cartoon (Indonesian Youth Story) obtained good criteria, and (4) the Feasibility of the Ceria Cartoon Calendar (Indonesian Youth Story) Design Feasibility criteria obtained very good eligibility criteria. The results of this study imply that the Ceria Cartoon Calendar (Indonesian Youth Story) Biology has good criteria to be used for adolescent students, especially in class IX SMP in conveying information about knowledge about sex education in supporting reproductive system materials in the classroom.
{"title":"KARTUN CERIA (CERITA REMAJA INDONESIA) TENTANG PENDIDIKAN SEKS SEBAGAI PENUNJANG MATERI AJAR SISTEM REPRODUKSI DI SMP","authors":"Hafizah Saleh, Binari Manurung, Ely Djulia","doi":"10.31258/biogenesis.17.2.69-80","DOIUrl":"https://doi.org/10.31258/biogenesis.17.2.69-80","url":null,"abstract":"This development research aims to determine: (1) Content Feasibility; (2) Feasibility of Presentation, (3) Feasibility of Language (Readability), and (4) Feasibility of Design of Ceria Cartoon Calendar (Indonesian Youth Story) for Junior High School students. The research procedure uses the Thiagarajan development model which consists of four stages (four-D models), namely: define stage, design stage, develop stage and disseminate. The feasibility analysis was carried out using a questionnaire instrument which was assessed by three parties consisting of Expert Validators, Biology Teacher Reviewers, and a Student Perception Questionnaire of the Cheerful Cartoon Calendar (Indonesian Youth Story) Biology Class IX SMP PGRI 24 Medan. Analysis. The results of this study showed that: (1) The Feasibility of the Contents of the Ceria Cartoon Calendar (Indonesian Youth Story) obtained good criteria, (2) the Feasibility of the Presentation of the Cheerful Cartoon Calendar (Indonesian Youth Story) obtained very good criteria, (3) the Feasibility of Language (Readability) Calendar Ceria Cartoon (Indonesian Youth Story) obtained good criteria, and (4) the Feasibility of the Ceria Cartoon Calendar (Indonesian Youth Story) Design Feasibility criteria obtained very good eligibility criteria. The results of this study imply that the Ceria Cartoon Calendar (Indonesian Youth Story) Biology has good criteria to be used for adolescent students, especially in class IX SMP in conveying information about knowledge about sex education in supporting reproductive system materials in the classroom.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90416497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-23DOI: 10.31258/biogenesis.17.2.49-60
Kandida Prajnaparamita, S. Susanti
Melinjo seeds (Gnetum gnemon L.) have many benefits, that it is necessary to know its morphological and anatomical characters. This study aimed to determine differences in morphological characters and anatomical development of melinjo seeds at four seed maturity stages. The morphological observation was carried out based on the quantitative and organoleptic characteristics of the melinjo seeds: outer envelope, size, and the color of the middle envelope. Seed development was anatomically observed in slides prepared with a non-embedding method using a sliding microtome then observed through a microscope. The outer seed envelope has a green to blackish-red color in the final stage, while the seed middle envelope has a light-brown to dark-brown in the final stage. The inner seed envelope is thin, non-rigid, and attached on the outside of the endosperm. The seeds' length ranges from ±1,5 cm until ±2,25 cm at the end-stage; seeds width are 1 cm – 1,18 cm; seeds diameter are 1 cm – 1,16 cm. The anatomical development showed tissue thickening and differentiation. The middle envelope is getting thicker: 318,84 μm to 397,29 μm. Endosperm tissue cells undergo cell compaction as the seeds ripen. At the same time, embryonic tissue differentiation forms hypocotyl, epicotyl, and cotyledon.
Melinjo种子(Gnetum gnemon L.)具有许多益处，有必要了解其形态和解剖特征。摘要本研究旨在探讨不同种子成熟期melinjo种子形态特征和解剖发育的差异。形态学观察主要依据甜椒种子的数量和感官特征:外包膜、大小和中包膜的颜色。在非包埋方法制备的载玻片上用滑动切片机解剖观察种子发育，然后通过显微镜观察。末期外种皮呈绿色至黑红色，末期种子中部种皮呈浅棕色至深棕色。种子的内层被膜很薄，不坚硬，附着在胚乳的外面。末期种子长度为±1.5 cm ~±2.25 cm;种子宽度为1厘米- 1.18厘米;种子直径为1厘米- 1.16厘米。解剖发育表现为组织增厚和分化。中间包层厚度从318,84 μm增加到397,29 μm。胚乳组织细胞在种子成熟时进行细胞压实。同时胚胎组织分化形成下胚轴、上胚轴和子叶。
{"title":"KARAKTER MORFOLOGIS DAN PERKEMBANGAN ANATOMIS BIJI MELINJO (Gnetum gnemon L.)","authors":"Kandida Prajnaparamita, S. Susanti","doi":"10.31258/biogenesis.17.2.49-60","DOIUrl":"https://doi.org/10.31258/biogenesis.17.2.49-60","url":null,"abstract":"Melinjo seeds (Gnetum gnemon L.) have many benefits, that it is necessary to know its morphological and anatomical characters. This study aimed to determine differences in morphological characters and anatomical development of melinjo seeds at four seed maturity stages. The morphological observation was carried out based on the quantitative and organoleptic characteristics of the melinjo seeds: outer envelope, size, and the color of the middle envelope. Seed development was anatomically observed in slides prepared with a non-embedding method using a sliding microtome then observed through a microscope. The outer seed envelope has a green to blackish-red color in the final stage, while the seed middle envelope has a light-brown to dark-brown in the final stage. The inner seed envelope is thin, non-rigid, and attached on the outside of the endosperm. The seeds' length ranges from ±1,5 cm until ±2,25 cm at the end-stage; seeds width are 1 cm – 1,18 cm; seeds diameter are 1 cm – 1,16 cm. The anatomical development showed tissue thickening and differentiation. The middle envelope is getting thicker: 318,84 μm to 397,29 μm. Endosperm tissue cells undergo cell compaction as the seeds ripen. At the same time, embryonic tissue differentiation forms hypocotyl, epicotyl, and cotyledon.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83890154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The main protease of the SARS-CoV-2 virus, SARS-CoV-2 Mpro, can be discovered as a promising target to treat the COVID-19 pandemic. The peptide-based inhibitors may present better options than small molecules for inhibits SARS-CoV-2 Mpro. Ziziphus spina-christi species reported have a peptide-based of alkaloids group, i.e. Amphibine that the analogues can be identified the potential as an inhibitor of SARS-CoV-2 Mpro. The compound structure was drawn and optimized using semi-empirical AM-1 method using Quantum ESPRESSO v.6.6, then the biological activity using PASS Prediction server and molecular docking simulation using MGLTools 1.5.6 with AutoDock 4.2 were performed. Afterward, the ADME profiles were predicted using the SWISS-ADME server. PASS server was predicting Amphibine B-F and H showed potency both as antiviral and as a protease inhibitor. The molecular docking simulation of Amphibine analogues showed lower binding energy than the native ligand. The binding energy of the native ligand was −7.69 Kcal/mol compared to the lowest binding energy of Amphibine analogues was −10.10 Kcal/mol (Amphibine-F). The ADME prediction showed, as an oral drug Amphibine-F has the best bioavailability, Amphibine-B, C, and D have good bioavailability, and Amphibine-E and H have poor bioavailability. Concluded, Amphibine B-F and H of Amphibine analogues showed potency as COVID-19 treatment targeting SARS-CoV-2 Mpro.
{"title":"Biological activity, molecular docking, and ADME predictions of amphibine analogues of Ziziphus spina-christi towards SARS-CoV-2 Mpro","authors":"T. M. Fakih, D. Ramadhan, F. Darusman","doi":"10.24252/bio.v9i1.21335","DOIUrl":"https://doi.org/10.24252/bio.v9i1.21335","url":null,"abstract":"The main protease of the SARS-CoV-2 virus, SARS-CoV-2 Mpro, can be discovered as a promising target to treat the COVID-19 pandemic. The peptide-based inhibitors may present better options than small molecules for inhibits SARS-CoV-2 Mpro. Ziziphus spina-christi species reported have a peptide-based of alkaloids group, i.e. Amphibine that the analogues can be identified the potential as an inhibitor of SARS-CoV-2 Mpro. The compound structure was drawn and optimized using semi-empirical AM-1 method using Quantum ESPRESSO v.6.6, then the biological activity using PASS Prediction server and molecular docking simulation using MGLTools 1.5.6 with AutoDock 4.2 were performed. Afterward, the ADME profiles were predicted using the SWISS-ADME server. PASS server was predicting Amphibine B-F and H showed potency both as antiviral and as a protease inhibitor. The molecular docking simulation of Amphibine analogues showed lower binding energy than the native ligand. The binding energy of the native ligand was −7.69 Kcal/mol compared to the lowest binding energy of Amphibine analogues was −10.10 Kcal/mol (Amphibine-F). The ADME prediction showed, as an oral drug Amphibine-F has the best bioavailability, Amphibine-B, C, and D have good bioavailability, and Amphibine-E and H have poor bioavailability. Concluded, Amphibine B-F and H of Amphibine analogues showed potency as COVID-19 treatment targeting SARS-CoV-2 Mpro.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78985668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microalgae Chlorella vulgaris is a microscopic green algae that has known potential as a source of food and medicine (functional food). However, the pharmacological capacity of this microalgae species against disease due to metabolic disorders is still not understood. Thus, this study aims to evaluate the effect of C. vulgaris extract on animal models of Drosophila melanogaster (Drosophila) which are fed high-sucrose diet (HSD). Wild type Drosophila was used in this study for 7 days of observation. Drosophila was divided into four groups consisting of the first group that was not treated as a control. The second group was treated with HSD (30%), the third group was treated with C. vulgaris extract (120 g/L) and the fourth group was treated with double treatment (HSD and C. vulgaris extract). The parameters observed included fecundity, hatchability, hemolymph glucose and triglyceride levels. The results showed that the extract of C. vulgaris was able to reduce the negative effects of giving HSD. The single treatment of C. vulgaris extract and double treatment between C. vulgaris extract and HSD significantly decreased levels of hemolymph glucose, triglycerides of Drosophila, while the parameters of fecundity and hatchability were significantly increased when compared to Drosophila which only received HSD treatment. The conclusion of this study is that C. vulgaris extract can be used as an antihyperglycemic agent which requires further study to prove the results that have been obtained.
{"title":"Effect of Chlorella vulgaris extract on high sucrose diet-induced diabetes in Drosophila melanogaster","authors":"Mohamad Agus Salim, M. Subandi, Y. Yuniarti","doi":"10.24252/bio.v9i1.18108","DOIUrl":"https://doi.org/10.24252/bio.v9i1.18108","url":null,"abstract":"Microalgae Chlorella vulgaris is a microscopic green algae that has known potential as a source of food and medicine (functional food). However, the pharmacological capacity of this microalgae species against disease due to metabolic disorders is still not understood. Thus, this study aims to evaluate the effect of C. vulgaris extract on animal models of Drosophila melanogaster (Drosophila) which are fed high-sucrose diet (HSD). Wild type Drosophila was used in this study for 7 days of observation. Drosophila was divided into four groups consisting of the first group that was not treated as a control. The second group was treated with HSD (30%), the third group was treated with C. vulgaris extract (120 g/L) and the fourth group was treated with double treatment (HSD and C. vulgaris extract). The parameters observed included fecundity, hatchability, hemolymph glucose and triglyceride levels. The results showed that the extract of C. vulgaris was able to reduce the negative effects of giving HSD. The single treatment of C. vulgaris extract and double treatment between C. vulgaris extract and HSD significantly decreased levels of hemolymph glucose, triglycerides of Drosophila, while the parameters of fecundity and hatchability were significantly increased when compared to Drosophila which only received HSD treatment. The conclusion of this study is that C. vulgaris extract can be used as an antihyperglycemic agent which requires further study to prove the results that have been obtained.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76417232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The native orchid Coelogyne rochussenii de Vriese is under threat of extinction, so conservation measures need to be taken. In-situ conservation is constrained by time, resources and costs, and the appropriate conservation must be ex-situ. Therefore, This study aims to determine the most effective method for ex-situ conservation, especially basal medium and activated charcoal, in order to determine the fastest germination. The method used in this research was a factorial completely randomized design, consisting of four different basal medium treatments, Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + Vitamin medium. This treatment was combined with the application of activated charcoal at 4 different levels, with concentrations of 0.1,2.3 g/L-1, the parameters observed were germination day (day), germination percentage (%), and percentage of contamination (%). The results of this study concluded that the conservation of the Native orchid Coelogyne Rochussenii de Vriese using various growing mediums and activated charcoal had a significant effect on the growth of the embryo culture native orchid. Hyponex + Vitamin medium with 3 g/L-1 is able to accelerate germination days to 29.33 days, increase germination percentage to 92.06%, and reduce contamination to 0%.
{"title":"Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas","authors":"Pebra Heriansyah, Gusti Marlina","doi":"10.24252/bio.v9i1.21274","DOIUrl":"https://doi.org/10.24252/bio.v9i1.21274","url":null,"abstract":"The native orchid Coelogyne rochussenii de Vriese is under threat of extinction, so conservation measures need to be taken. In-situ conservation is constrained by time, resources and costs, and the appropriate conservation must be ex-situ. Therefore, This study aims to determine the most effective method for ex-situ conservation, especially basal medium and activated charcoal, in order to determine the fastest germination. The method used in this research was a factorial completely randomized design, consisting of four different basal medium treatments, Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + Vitamin medium. This treatment was combined with the application of activated charcoal at 4 different levels, with concentrations of 0.1,2.3 g/L-1, the parameters observed were germination day (day), germination percentage (%), and percentage of contamination (%). The results of this study concluded that the conservation of the Native orchid Coelogyne Rochussenii de Vriese using various growing mediums and activated charcoal had a significant effect on the growth of the embryo culture native orchid. Hyponex + Vitamin medium with 3 g/L-1 is able to accelerate germination days to 29.33 days, increase germination percentage to 92.06%, and reduce contamination to 0%.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77803680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Developing to friendly, greenery, and sustain in the modern era is not easy to implement, because of development is more depends on economic profit, which has exploited massively and destructively to welfare or wellbeing achieved. Those phenomena are part of one symptom to environmental or land degradation occurrence accumulatively, so indirectly or directly it caused of interest conflict in competing to natural resources that diminished. Hence, it needed an alternative solution, which one is conservation acted in land restoring kindly and effectively and of course based on religions (Islamic) approach. Exploring and understanding verses (The Holy Qur’an) related to land management to conservation, which is a method that used in collecting data and it's used to analysis, alongside observation and in-depth individual interview is also to strengthen a scientific argument based on field data result. The result of this research revealed that the boarding school of Al-Zaytun is one of all examples of success in land and water management to conservation and it’s able to food, water, and energy security development kindly and sustains. Empirically that Pesantren Al-Zaytun is able to both manage and utilize waste (water) kindly and ecologically, which is reuse, reduce, and recycle development paradigm, so it has become of example to development in the modern era, alongside its ability to representative developed in utilizing to natural resources management for development ecologically.
{"title":"Eco Pesantren development: Challenges and opportunities to conservation development","authors":"K. Kristiyanto, H. Alikodra","doi":"10.24252/bio.v9i1.21084","DOIUrl":"https://doi.org/10.24252/bio.v9i1.21084","url":null,"abstract":"Developing to friendly, greenery, and sustain in the modern era is not easy to implement, because of development is more depends on economic profit, which has exploited massively and destructively to welfare or wellbeing achieved. Those phenomena are part of one symptom to environmental or land degradation occurrence accumulatively, so indirectly or directly it caused of interest conflict in competing to natural resources that diminished. Hence, it needed an alternative solution, which one is conservation acted in land restoring kindly and effectively and of course based on religions (Islamic) approach. Exploring and understanding verses (The Holy Qur’an) related to land management to conservation, which is a method that used in collecting data and it's used to analysis, alongside observation and in-depth individual interview is also to strengthen a scientific argument based on field data result. The result of this research revealed that the boarding school of Al-Zaytun is one of all examples of success in land and water management to conservation and it’s able to food, water, and energy security development kindly and sustains. Empirically that Pesantren Al-Zaytun is able to both manage and utilize waste (water) kindly and ecologically, which is reuse, reduce, and recycle development paradigm, so it has become of example to development in the modern era, alongside its ability to representative developed in utilizing to natural resources management for development ecologically. ","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80596304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lulut Dwi Sulistyaningsih, A. Abinawanto, M. Ardiyani, A. Salamah, Agus Haryadi
Smilacaceae is known as a taxa with wide phenotypic variation and their taxonomical complexities remain unsolved. The three species of Smilacaceae housed in Java, are given nomenclature history. Since all the potential sources of original material have been investigated, but nothing has been identified, three neotypifications were designated here. Herbarium specimen of Koorders 34990β in Herbarium Bogoriense (BO) was chosen as the neotype of Smilax klotzschii. The de Groot & Wehlburg RD52 herbarium specimen in BO was chosen as the neotype of S. nageliana and Blume 463 herbarium specimen in L was chosen as the neotype of S. odoratissima.
笑脸科是一个具有广泛表型变异的分类群，其分类复杂性尚未得到解决。介绍了爪哇三种菝葜科植物的命名史。由于已调查了所有可能的原始材料来源，但没有发现任何东西，因此此处指定了三种新类型。选择波哥大植物标本馆(BO)的Koorders 34990β标本为菝葜新种。选择BO的de Groot & Wehlburg RD52标本馆标本作为nageliana新型，L的Blume 463标本馆标本作为odoratissima新型。
{"title":"Typification and taxonomic notes of Smilacaceae species in Java","authors":"Lulut Dwi Sulistyaningsih, A. Abinawanto, M. Ardiyani, A. Salamah, Agus Haryadi","doi":"10.24252/bio.v9i1.17218","DOIUrl":"https://doi.org/10.24252/bio.v9i1.17218","url":null,"abstract":"Smilacaceae is known as a taxa with wide phenotypic variation and their taxonomical complexities remain unsolved. The three species of Smilacaceae housed in Java, are given nomenclature history. Since all the potential sources of original material have been investigated, but nothing has been identified, three neotypifications were designated here. Herbarium specimen of Koorders 34990β in Herbarium Bogoriense (BO) was chosen as the neotype of Smilax klotzschii. The de Groot & Wehlburg RD52 herbarium specimen in BO was chosen as the neotype of S. nageliana and Blume 463 herbarium specimen in L was chosen as the neotype of S. odoratissima.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77451207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Parwanayoni, Dewa Ngurah Suprapta, Nyoman Darsini, S. K. Sudirga
Efforts to improve food security in Indonesia, especially in Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Legume crops are often attacked by stem rot diseases which can cause decreased production and economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of the two species of the fungus Sclerotium rolfsii and Athelia rolfsii are the same and difficult to distinguish, so molecular identification is needed to determine the species. The research aims to isolate and molecularly identify fungi that cause stem rot disease in local legume plants in Bali. Research methods include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease in Bali, pathogenicity test, identification of the highest levels of virulent isolates, DNA extraction, DNA amplification by PCR, and electrophoresis, ITS region sequencing, and computer analysis sequences DNA. The results of isolation pathogens that cause stem rot disease in Bali's local legume plants obtained six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate after the pathogenicity test of peanut plants. Molecular identification results show that SKT isolate is Athelia rolfsii, because it's in a clade with Athelia rolfsii fungi sequences in GenBank with 100% Bootstrap support.
{"title":"Isolation and molecular identification of fungi that cause stem rot disease in Bali's local legumes","authors":"N. Parwanayoni, Dewa Ngurah Suprapta, Nyoman Darsini, S. K. Sudirga","doi":"10.24252/bio.v9i1.20426","DOIUrl":"https://doi.org/10.24252/bio.v9i1.20426","url":null,"abstract":"Efforts to improve food security in Indonesia, especially in Bali, need to be supported by improvements in cultivation techniques, including the management of pests and diseases. Legume crops are often attacked by stem rot diseases which can cause decreased production and economic losses. This disease is generally caused by the soil-borne pathogenic fungus Sclerotium rolfsii or Athelia rolfsii. The macroscopic and microscopic morphologies of the two species of the fungus Sclerotium rolfsii and Athelia rolfsii are the same and difficult to distinguish, so molecular identification is needed to determine the species. The research aims to isolate and molecularly identify fungi that cause stem rot disease in local legume plants in Bali. Research methods include isolation of pathogenic fungi from legumes showing symptoms of stem rot disease in Bali, pathogenicity test, identification of the highest levels of virulent isolates, DNA extraction, DNA amplification by PCR, and electrophoresis, ITS region sequencing, and computer analysis sequences DNA. The results of isolation pathogens that cause stem rot disease in Bali's local legume plants obtained six fungal isolates coded SKT, SKB1, SKB2 SKB3, SKL and SKN isolates. SKT isolates had the highest virulence rate after the pathogenicity test of peanut plants. Molecular identification results show that SKT isolate is Athelia rolfsii, because it's in a clade with Athelia rolfsii fungi sequences in GenBank with 100% Bootstrap support.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"520 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77057134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Low phosphate content in the soil can cause insufficient plant needs. Besides, the endophytic fungi of nutgrass have the potential as a phosphate solvent and can produce IAA (Indole-3-Acetic Acid). This study aimed to determine the levels of solubilizing phosphate and production of IAA (Indole-3-Acetic Acid) by the endophytic fungi of nutgrass and to identify the isolates based on rDNA-ITS sequences. The methods used were the isolation of endophytic fungi, analysis of solubilizing levels of phosphate and IAA production, and molecular analysis with rDNA-ITS sequences. Results of isolation and purification, found five isolates coded URT1, URT2, URT3, URT4, and URT5. The endophytic fungi of nutgrass were able to solubilizing phosphate levels around 54.03 - 87.83 ppm, with the highest levels produced by URT4 isolate. IAA levels around 5.58 - 45.50 ppm, with the highest levels produced by URT1 isolate. Based on molecular analysis with rDNA-ITS sequences, it showed that URT4 had 97.42% similarity to Aspergillus tereus species, while UTR1 had 100% similarity to Fusarium oxyporum species. To conclude, the endophytic fungi of nutgrass from A. tereus and F. oxyporum species have high levels of solubilizing phosphate and IAA production so that they are potential candidates for biofertilizer.
{"title":"Identification endophytic fungi of Nutgrass (Cyperus rotundus L.) as solubilizing phosphate and indole-3-acetic acid producers","authors":"Nur Kusmiyati, Septian Tri Wicaksono, D. Maknuna","doi":"10.24252/bio.v9i1.21216","DOIUrl":"https://doi.org/10.24252/bio.v9i1.21216","url":null,"abstract":"Low phosphate content in the soil can cause insufficient plant needs. Besides, the endophytic fungi of nutgrass have the potential as a phosphate solvent and can produce IAA (Indole-3-Acetic Acid). This study aimed to determine the levels of solubilizing phosphate and production of IAA (Indole-3-Acetic Acid) by the endophytic fungi of nutgrass and to identify the isolates based on rDNA-ITS sequences. The methods used were the isolation of endophytic fungi, analysis of solubilizing levels of phosphate and IAA production, and molecular analysis with rDNA-ITS sequences. Results of isolation and purification, found five isolates coded URT1, URT2, URT3, URT4, and URT5. The endophytic fungi of nutgrass were able to solubilizing phosphate levels around 54.03 - 87.83 ppm, with the highest levels produced by URT4 isolate. IAA levels around 5.58 - 45.50 ppm, with the highest levels produced by URT1 isolate. Based on molecular analysis with rDNA-ITS sequences, it showed that URT4 had 97.42% similarity to Aspergillus tereus species, while UTR1 had 100% similarity to Fusarium oxyporum species. To conclude, the endophytic fungi of nutgrass from A. tereus and F. oxyporum species have high levels of solubilizing phosphate and IAA production so that they are potential candidates for biofertilizer.","PeriodicalId":8911,"journal":{"name":"Biogenesis: Jurnal Ilmiah Biologi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82353430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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