Pub Date : 2022-11-12DOI: 10.1080/10889868.2022.2143473
Ifeoma Anthonia Okpara-Elom, Charles Chike Onochie, Michael Okpara Elom, Emmanuel Ezaka, Ogbonnaya Elom
Abstract
Soil samples were collected from research farm of Ebonyi State University and mining site in Izzi Local Government Area (L.G.A) of Ebonyi State. Rhizobacteria from rhizosphere of maize were identified using molecular methods. Tolerance of the isolates to heavy metals was assessed using standard laboratory techniques. The heavy metal profile of the soil was determined using atomic absorption spectrophotometer (AAS). Ability of the PGPB to remediate heavy metal polluted soil was assessed with hot plate aqua-regiae digestion method. The isolates were identified as: Pseudomonas fluorescens strain 5113, Alcaligenes faecalis strain P156, Pseudomonas syringae HS191, Bacillus subtilis strain NBSL51 and Bacillus cereus strain 20 UPMNR. P. fluorescens exhibited maximum tolerance at different concentrations of metals while A. faecalis had minimum tolerance. The heavy metal profile of the soil was determined and expressed in ppm as lead (25.0), copper (2.4), chromium (1.2) and zinc (16.0). The Isolates remediated the heavy metals. P. fluorescens + B. cereus maximally degraded the metals with values of (Pb=87.2%, Zn=69.25%, Cr =67.5%) while B. cereus alone degraded 70.0% Cu. P. fluorescens + B. cereus is recommended for soil that is contaminated with chromium, lead and zinc while B. cereus is recommended for copper contaminated soils.
{"title":"Bioremediation of heavy metal polluted soil using plant growth promoting bacteria: an assessment of response","authors":"Ifeoma Anthonia Okpara-Elom, Charles Chike Onochie, Michael Okpara Elom, Emmanuel Ezaka, Ogbonnaya Elom","doi":"10.1080/10889868.2022.2143473","DOIUrl":"https://doi.org/10.1080/10889868.2022.2143473","url":null,"abstract":"<p><b>Abstract</b></p><p>Soil samples were collected from research farm of Ebonyi State University and mining site in Izzi Local Government Area (L.G.A) of Ebonyi State. Rhizobacteria from rhizosphere of maize were identified using molecular methods. Tolerance of the isolates to heavy metals was assessed using standard laboratory techniques. The heavy metal profile of the soil was determined using atomic absorption spectrophotometer (AAS). Ability of the PGPB to remediate heavy metal polluted soil was assessed with hot plate aqua-regiae digestion method. The isolates were identified as: <i>Pseudomonas fluorescens</i> strain 5113, <i>Alcaligenes faecalis</i> strain P156, <i>Pseudomonas syringae</i> HS191, <i>Bacillus subtilis</i> strain NBSL51 and <i>Bacillus cereus</i> strain 20 UPMNR. <i>P. fluorescens</i> exhibited maximum tolerance at different concentrations of metals while <i>A. faecalis</i> had minimum tolerance. The heavy metal profile of the soil was determined and expressed in ppm as lead (25.0), copper (2.4), chromium (1.2) and zinc (16.0). The Isolates remediated the heavy metals. <i>P. fluorescens</i> + <i>B. cereus</i> maximally degraded the metals with values of (Pb<b> </b>=<b> </b>87.2%, Zn<b> </b>=<b> </b>69.25%, Cr =67.5%) while <i>B. cereus</i> alone degraded 70.0% Cu. <i>P. fluorescens</i> + <i>B. cereus</i> is recommended for soil that is contaminated with chromium, lead and zinc while <i>B. cereus</i> is recommended for copper contaminated soils.</p>","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"24 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138524094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Stenotrophomonas koreensis, was isolated from a textile effluent and employed to biologically degrade 98.8% of the recalcitrant toxic dye malachite green in 4 h in the absence of any supplements or media. Chromatographic and Spectroscopic analysis confirmed the degradation of the dye. According to Response Surface Methodology, the optimum conditions for biodegradation were pH 6, inoculum size 6 mL (initial concentration Log10CFU/mL = 6.2), and temperature 45 °C. More than 98% decolorization was achieved within 2.5 h of the addition of soymeal extract or peptone. One of the most promising features of this strain is that even in the presence of heavy metals, S. koreensis actively degraded the dye. The bacteria biodegraded malachite green following the first-order reaction kinetics. LC-MS analysis of the degradation product yielded several intermediates like Michler’s ketone (m/z 269), 4-N,N-dimethylaminophenol (m/z 137), Benzophenone (m/z 182), N,N-dimethylaniline (m/z 121), 4-(N-methylamino)-benzophenone (m/z 211), 4-aminobenzaldehyde (m/z 121), anionic canonical form of 4-aminobenzaldehyde (m/z 120), 4-(N,N-dimethylamino)benzoic acid (m/z 165), 4-(N-methylamino) benzoic acid (m/z 152). The enzymes responsible for the malachite green degradation were tyrosinase, Malachite Green reductase, and NADH-DCIP reductase. The present work is the first to report the degradation of malachite green by S. koreensis.
{"title":"Enzymes analysis, degradation kinetics, response surface optimization and heavy metal tolerance of the biodegradation of malachite green by Stenotrophomonas koreensis","authors":"Shreya Biswas, Tuhin Kahali, Anwesha Mukherjee, Debasmita Chakraborty, Chayan Guha, Tathagata Adhikary, Pratik Das, Nandan Kumar Jana, Suvendu Manna, Piyali Basak","doi":"10.1080/10889868.2022.2143472","DOIUrl":"https://doi.org/10.1080/10889868.2022.2143472","url":null,"abstract":"<p><b>Abstract</b></p><p><i>Stenotrophomonas koreensis</i>, was isolated from a textile effluent and employed to biologically degrade 98.8% of the recalcitrant toxic dye malachite green in 4 h in the absence of any supplements or media. Chromatographic and Spectroscopic analysis confirmed the degradation of the dye. According to Response Surface Methodology, the optimum conditions for biodegradation were pH 6, inoculum size 6 mL (initial concentration Log<sub>10</sub>CFU/mL = 6.2), and temperature 45 °C. More than 98% decolorization was achieved within 2.5 h of the addition of soymeal extract or peptone. One of the most promising features of this strain is that even in the presence of heavy metals, <i>S. koreensis</i> actively degraded the dye. The bacteria biodegraded malachite green following the first-order reaction kinetics. LC-MS analysis of the degradation product yielded several intermediates like Michler’s ketone (m/z 269), 4-N,N-dimethylaminophenol (m/z 137), Benzophenone (m/z 182), N,N-dimethylaniline (m/z 121), 4-(N-methylamino)-benzophenone (m/z 211), 4-aminobenzaldehyde (m/z 121), anionic canonical form of 4-aminobenzaldehyde (m/z 120), 4-(N,N-dimethylamino)benzoic acid (m/z 165), 4-(N-methylamino) benzoic acid (m/z 152). The enzymes responsible for the malachite green degradation were tyrosinase, Malachite Green reductase, and NADH-DCIP reductase. The present work is the first to report the degradation of malachite green by <i>S. koreensis</i>.</p>","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":"31 6","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138524091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-11DOI: 10.1080/10889868.2022.2136134
Shailendra Ganna, Poonam R. Kulkarni, S. Garg, C. Newell
{"title":"Spatial-temporal trends and correlations from a large natural source zone depletion (NSZD) research project at a site with LNAPL","authors":"Shailendra Ganna, Poonam R. Kulkarni, S. Garg, C. Newell","doi":"10.1080/10889868.2022.2136134","DOIUrl":"https://doi.org/10.1080/10889868.2022.2136134","url":null,"abstract":"","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46654083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-01DOI: 10.1080/10889868.2022.2136132
M. Abdollahpour, M. Ahmadpour, M. Sinkakarimi, Salman Ahmady Asbchin, Khodadad Soltani, K. Khermandar, K. Kucharska, Łukasz J. Binkowski
{"title":"Humic acid reduces lead phytoextraction efficiency of Erythrostemon gilliesii","authors":"M. Abdollahpour, M. Ahmadpour, M. Sinkakarimi, Salman Ahmady Asbchin, Khodadad Soltani, K. Khermandar, K. Kucharska, Łukasz J. Binkowski","doi":"10.1080/10889868.2022.2136132","DOIUrl":"https://doi.org/10.1080/10889868.2022.2136132","url":null,"abstract":"","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46817817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-31DOI: 10.1080/10889868.2022.2138256
M. Saad, Mai A. Saad, Bedour S. Saad, Fatma A. Zakaria, Abdel-Rahman A. Husain, S. Abdelgaleil
{"title":"Bioremediation and microbial-assisted phytoremediation of heavy metals by endophytic Fusarium species isolated from Convolvulus arvensis","authors":"M. Saad, Mai A. Saad, Bedour S. Saad, Fatma A. Zakaria, Abdel-Rahman A. Husain, S. Abdelgaleil","doi":"10.1080/10889868.2022.2138256","DOIUrl":"https://doi.org/10.1080/10889868.2022.2138256","url":null,"abstract":"","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48754584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-20DOI: 10.1080/10889868.2022.2136133
H. Mukhtar, Iram Hina, B. Muneer, Umar Farooq Gohar, M. Akhtar
{"title":"Kinetics of the biosorptive removal of chromium from water using mycelial biomass of Aspergillus oryzae","authors":"H. Mukhtar, Iram Hina, B. Muneer, Umar Farooq Gohar, M. Akhtar","doi":"10.1080/10889868.2022.2136133","DOIUrl":"https://doi.org/10.1080/10889868.2022.2136133","url":null,"abstract":"","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42898136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-12DOI: 10.1080/10889868.2022.2130874
M. Omeiri, Rony S. Khnayzer, H. Yusef, S. Tokajian, Tamara Salloum
{"title":"Biodegradation of chlorpyrifos by bacterial strains isolated from Lebanese soil and its association with plant growth improvement","authors":"M. Omeiri, Rony S. Khnayzer, H. Yusef, S. Tokajian, Tamara Salloum","doi":"10.1080/10889868.2022.2130874","DOIUrl":"https://doi.org/10.1080/10889868.2022.2130874","url":null,"abstract":"","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49610965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-06DOI: 10.1080/10889868.2022.2130873
Ifeanyi Michael Smarte Anekwe, Y. Isa
Abstract Acid mine drainage (AMD) poses a serious challenge to the ecosystem due to its disastrous effect on soil and water resources which contributes to the pollution and reduction of the accessibility of potable water and arable land for agricultural activities. The treatment of contaminated soils using physical, chemical, or thermal methods requires some improvements to meet the required remediation purpose. The purpose of this study is to investigate the application of bioattenuation, wastewater, and air-injection for the remediation of AMD polluted soils. A microcosm comprising 1 kg of polluted soil was amended with varying loadings of domestic and brewery wastewaters for biostimulation treatments, and the bioattenuation received no amendment while the bioventing treatment was supplied with wastewaters and atmospheric air at 3 L/min for 30 mins intervals every 48 h. The investigation conducted under the ambient condition for 28 days recorded average metal removal efficiencies of 12–31% (bioattenuation), 50–66% for all heavy metals (iron, aluminum, copper and zinc) except manganese (28%) (biostimulation) and 56–70% (bioventing) while 32%, 35% and 52% sulfate removal efficiencies were recorded for bioattenuation, bioventing and biostimulation respectively. The study revealed that wastewater and bioventing systems can be efficiently utilized as an eco-friendly approach for the treatment of acid mine contaminated soils and pollution mitigation in the mining environments. In addition, from a comparative point of view, the results of the study further showed that the amendment of wastewater is more effective for sulfate removal while air injection enhanced metal removal from acid mine drainage contaminated soils. The synergetic effects have also been observed; the current study demonstrates that the combined application of biostimulation and bioventing system requires constant monitoring to ensure the provision of appropriate anoxic conditions for biosulfidogenic activity by enhancing sulfate removal while promoting an oxic environment for the oxidation process to facilitate metal removal for the effective treatment of acid mine drainage contaminated soil.
{"title":"Bioremediation of acid mine drainage contaminated soils using bioattenuation, wastewater and air-injection system","authors":"Ifeanyi Michael Smarte Anekwe, Y. Isa","doi":"10.1080/10889868.2022.2130873","DOIUrl":"https://doi.org/10.1080/10889868.2022.2130873","url":null,"abstract":"Abstract Acid mine drainage (AMD) poses a serious challenge to the ecosystem due to its disastrous effect on soil and water resources which contributes to the pollution and reduction of the accessibility of potable water and arable land for agricultural activities. The treatment of contaminated soils using physical, chemical, or thermal methods requires some improvements to meet the required remediation purpose. The purpose of this study is to investigate the application of bioattenuation, wastewater, and air-injection for the remediation of AMD polluted soils. A microcosm comprising 1 kg of polluted soil was amended with varying loadings of domestic and brewery wastewaters for biostimulation treatments, and the bioattenuation received no amendment while the bioventing treatment was supplied with wastewaters and atmospheric air at 3 L/min for 30 mins intervals every 48 h. The investigation conducted under the ambient condition for 28 days recorded average metal removal efficiencies of 12–31% (bioattenuation), 50–66% for all heavy metals (iron, aluminum, copper and zinc) except manganese (28%) (biostimulation) and 56–70% (bioventing) while 32%, 35% and 52% sulfate removal efficiencies were recorded for bioattenuation, bioventing and biostimulation respectively. The study revealed that wastewater and bioventing systems can be efficiently utilized as an eco-friendly approach for the treatment of acid mine contaminated soils and pollution mitigation in the mining environments. In addition, from a comparative point of view, the results of the study further showed that the amendment of wastewater is more effective for sulfate removal while air injection enhanced metal removal from acid mine drainage contaminated soils. The synergetic effects have also been observed; the current study demonstrates that the combined application of biostimulation and bioventing system requires constant monitoring to ensure the provision of appropriate anoxic conditions for biosulfidogenic activity by enhancing sulfate removal while promoting an oxic environment for the oxidation process to facilitate metal removal for the effective treatment of acid mine drainage contaminated soil.","PeriodicalId":8935,"journal":{"name":"Bioremediation Journal","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2022-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44121935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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