ACS Biomaterials Science & Engineering最新文献

Improvements in tumor therapy require a combination of strategies where targeted treatment is critical. We developed a new versatile nanoplatform, MA@E, that generates high levels of reactive oxygen species (ROS) with effective photothermal conversions in the removal of tumors. Enhanced stability liposomes were employed as carriers to facilitate the uniform distribution and stable storage of encapsulated gold nanorods (AuNRs) and Mn-MIL-100 metal-organic frameworks, with efficient delivery of MA@E to the cytoplasm. In the targeted phagocytosis of tumor cells, MA@E can effectively deplete the reduced glutathione (GSH) with increased hydroxyl radicals that combine with Mn²⁺ released from Mn-MIL-100 to trigger Fenton-like reactions, generating ROS that induces cell apoptosis. Exposure to near-infrared (NIR-II) irradiation results in a AuNRs-induced thermogenic effect that expedites the release of Mn²⁺ and promotes Fenton-like reactions, achieving increased production of ^•OH. In the murine tumor model, MA@E effectively removed the implanted tumor tissue within 2 days without any obvious toxic effects. This response is attributed to a synergism involving the photothermal capability of AuNRs and ROS chemodynamic treatment. The proposed MA@E provides a new approach to utilizing unstable nanomaterials in effective tumor therapy.

Roughly 1.71 billion people worldwide suffer from large bone abnormalities, which are the primary cause of disability. Traditional bone grafting procedures have several drawbacks that impair their therapeutic efficacy and restrict their use in clinical settings. A great deal of work has been done to create fresh, more potent strategies. Under these circumstances, a crucial technique for the regeneration of major lesions has emerged: bone tissue engineering (BTE). BTE involves the use of biomaterials that can imitate the natural design of bone. To yet, no biological material has been able to fully meet the parameters of the perfect implantable material, even though several varieties have been created and investigated for bone regeneration. Against this backdrop, researchers have focused a great deal of interest over the past few years on the subject of nanotechnology and the use of nanostructures in regenerative medicine. The ability to create nanoengineered particles that can overcome the current constraints in regenerative strategies─such as decreased cell proliferation and differentiation, insufficient mechanical strength in biological materials, and insufficient production of extrinsic factors required for effective osteogenesis has revolutionized the field of bone and tissue engineering. The effects of nanoparticles on cell characteristics and the application of biological materials for bone regeneration are the main topics of our review, which summarizes the most recent in vitro and in vivo research on the application of nanotechnology in the context of BTE.

Magnesium alloys are often used in bone repair surgeries due to their biodegradability and excellent elastic modulus, making them a promising alternative to traditional nondegradable implants like titanium alloys. However, their rapid degradation rate limits their use as implants in the body. To enhance the corrosion resistance and bioactivity of magnesium alloys, we applied an ultrasonic spray coating on microarc oxidized (MAO) AZ31 magnesium alloy, using a mixture of silk fibroin (SF) and nanohydroxyapatite (nHAp). This SF/nHAp composite embeds directly into the micropores on the MAO-treated surface without additional physical or chemical treatment, forming a stable interlocked coating structure. The effects of different spray parameters on coating adhesion and interface characteristics were investigated, leading to the development of a corrosion-resistant and highly biocompatible composite coating. Further biological evaluations were conducted through subcutaneous implantation, assessing the in vivo degradation of the samples and the surrounding tissue response from multiple perspectives. A novel concept of in vivo tissue-reactive coatings was proposed, suggesting that highly biocompatible coating materials, in the early stages postimplantation, enable surrounding fibrous tissues to closely adhere to the surface, thereby slowing material degradation. As a result, the highly bioactive MAO-SF/nHAp coating significantly enhances the corrosion resistance of magnesium alloys, reduces hydrogen evolution, promotes regeneration of surrounding tissues, and minimizes postimplant inflammation. This approach offers a new strategy to improve the biocompatibility and corrosion resistance of magnesium alloys in vivo, suggesting that the overall evaluation of biodegradable magnesium alloys should focus more on assessing in-body corrosion.

Roughly 1.71 billion people worldwide suffer from large bone abnormalities, which are the primary cause of disability. Traditional bone grafting procedures have several drawbacks that impair their therapeutic efficacy and restrict their use in clinical settings. A great deal of work has been done to create fresh, more potent strategies. Under these circumstances, a crucial technique for the regeneration of major lesions has emerged: bone tissue engineering (BTE). BTE involves the use of biomaterials that can imitate the natural design of bone. To yet, no biological material has been able to fully meet the parameters of the perfect implantable material, even though several varieties have been created and investigated for bone regeneration. Against this backdrop, researchers have focused a great deal of interest over the past few years on the subject of nanotechnology and the use of nanostructures in regenerative medicine. The ability to create nanoengineered particles that can overcome the current constraints in regenerative strategies─such as decreased cell proliferation and differentiation, insufficient mechanical strength in biological materials, and insufficient production of extrinsic factors required for effective osteogenesis has revolutionized the field of bone and tissue engineering. The effects of nanoparticles on cell characteristics and the application of biological materials for bone regeneration are the main topics of our review, which summarizes the most recent in vitro and in vivo research on the application of nanotechnology in the context of BTE.

Magnesium alloys are often used in bone repair surgeries due to their biodegradability and excellent elastic modulus, making them a promising alternative to traditional nondegradable implants like titanium alloys. However, their rapid degradation rate limits their use as implants in the body. To enhance the corrosion resistance and bioactivity of magnesium alloys, we applied an ultrasonic spray coating on microarc oxidized (MAO) AZ31 magnesium alloy, using a mixture of silk fibroin (SF) and nanohydroxyapatite (nHAp). This SF/nHAp composite embeds directly into the micropores on the MAO-treated surface without additional physical or chemical treatment, forming a stable interlocked coating structure. The effects of different spray parameters on coating adhesion and interface characteristics were investigated, leading to the development of a corrosion-resistant and highly biocompatible composite coating. Further biological evaluations were conducted through subcutaneous implantation, assessing the in vivo degradation of the samples and the surrounding tissue response from multiple perspectives. A novel concept of in vivo tissue-reactive coatings was proposed, suggesting that highly biocompatible coating materials, in the early stages postimplantation, enable surrounding fibrous tissues to closely adhere to the surface, thereby slowing material degradation. As a result, the highly bioactive MAO-SF/nHAp coating significantly enhances the corrosion resistance of magnesium alloys, reduces hydrogen evolution, promotes regeneration of surrounding tissues, and minimizes postimplant inflammation. This approach offers a new strategy to improve the biocompatibility and corrosion resistance of magnesium alloys in vivo, suggesting that the overall evaluation of biodegradable magnesium alloys should focus more on assessing in-body corrosion.

Improvements in tumor therapy require a combination of strategies where targeted treatment is critical. We developed a new versatile nanoplatform, MA@E, that generates high levels of reactive oxygen species (ROS) with effective photothermal conversions in the removal of tumors. Enhanced stability liposomes were employed as carriers to facilitate the uniform distribution and stable storage of encapsulated gold nanorods (AuNRs) and Mn-MIL-100 metal–organic frameworks, with efficient delivery of MA@E to the cytoplasm. In the targeted phagocytosis of tumor cells, MA@E can effectively deplete the reduced glutathione (GSH) with increased hydroxyl radicals that combine with Mn²⁺ released from Mn-MIL-100 to trigger Fenton-like reactions, generating ROS that induces cell apoptosis. Exposure to near-infrared (NIR-II) irradiation results in a AuNRs-induced thermogenic effect that expedites the release of Mn²⁺ and promotes Fenton-like reactions, achieving increased production of ^•OH. In the murine tumor model, MA@E effectively removed the implanted tumor tissue within 2 days without any obvious toxic effects. This response is attributed to a synergism involving the photothermal capability of AuNRs and ROS chemodynamic treatment. The proposed MA@E provides a new approach to utilizing unstable nanomaterials in effective tumor therapy.

Intervertebral disc degeneration (IVDD) is a major contributor to chronic back pain and disability, with limited effective therapeutic options. Current treatment options, including conservative management and surgical interventions, often fail to effectively halt disease progression and come with notable side effects. IVDD is characterized by the breakdown of the extracellular matrix (ECM) and the infiltration of inflammatory cells, which exacerbate disc degeneration. This study presents a novel therapeutic strategy aimed at addressing the dual challenges of inflammation and ECM degradation in IVDD. We developed a gelatin methacryloyl (GelMA) hydrogel system loaded with interleukin-10 (IL-10), an anti-inflammatory cytokine, and kartogenin (KGN), a small-molecule compound known for its regenerative properties. The KGN + IL-10@GelMA hydrogel was designed to deliver these agents in a controlled manner directly to the degenerated disc, targeting both the inflammatory microenvironment and the promotion of nucleus pulposus (NP) tissue regeneration. In a puncture-induced IVDD model, this hydrogel system effectively delayed the degenerative progression and facilitated NP regeneration. Our findings suggest that the KGN + IL-10@GelMA hydrogel holds significant potential as a nonsurgical treatment option for IVDD, offering a promising approach to mitigate the progression of IVDD and enhance disc repair.

The combination of green manufacturing approaches and bioinspired materials is growingly emerging in different scenarios, in particular in medicine, where widespread medical devices (MDs) as commercial electrodes for electrophysiology strongly increase the accumulation of solid waste after use. Electrocardiogram (ECG) electrodes exploit electrolytic gels to allow the high-quality recording of heart signals. Beyond their nonrecyclability/nonrecoverability, gel drying also affects the signal quality upon prolonged monitoring of biopotentials. Moreover, gel composition often causes skin reactions. This study aims to address the above limitation by presenting a composite based on the combination of silk sericin (SS) as a structural material, poly(vinyl alcohol) (PVA) as a robustness enhancer, and CaCl₂ as a plasticizer. SS/PVA/CaCl₂ formulations, optimized in terms of weight content (wt %) of single constituents, result in a biocompatible, biodegradable "green" material (free from potentially irritating cross-linking agents) that is, above all, self-adhesive on skin. The best formulation, i.e., SS(4 wt %)/PVA(4 wt %)/CaCl₂(20 wt %), in terms of long-lasting skin adhesion (favored by calcium-ion coordination in the presence of environmental/skin humidity) and time-stability of electrode impedance, is used to assemble ECG electrodes showing quality trace recording over longer time scales (up to 6 h) than commercial electrodes. ECG recording is performed using customized electronics coupled to an app for data visualization.

The impaired healing of alveolar bone defects in diabetic patients has attracted considerable attention, with Mogroside V (MV) emerging as a promising candidate due to its demonstrated antioxidation, hypoglycemic, and anti-inflammatory properties in patients with diabetes mellitus. To address the limitations of oral MV administration, such as low bioavailability, rapid metabolism, and a short half-life, we developed a nanofiber membrane utilizing electrospinning technology for topical application by preparing membranes using MV, chitosan (CS), nanohydroxyapatite (HA), and poly(vinyl alcohol) (PVA) as raw materials to prolong the effect of MV and enhance bone regeneration in diabetic patients. The MV/HA/PVA/CS exhibited a good fiber diameter, prolonged drug release, and suitable degradation time, along with other favorable properties. In vitro experiments revealed its excellent biocompatibility, effectiveness in promoting osteogenesis, upregulation of osteogenic and anti-inflammatory genes, and concurrent downregulation of pro-inflammatory genes. In vivo evaluations further confirmed its ability to effectively modulate the diabetic microenvironment, reduce bone damage, and facilitate anti-inflammatory effects and alveolar bone regeneration in diabetics. These findings suggest that a nanofiber membrane with sustained release of MV may serve as a promising biomaterial, providing new insights into improving the healing of diabetic alveolar bone defects.

Radiofrequency (RF) tissue welding is an innovative tissue anastomosis technique that utilizes bioimpedance to convert electrical energy into thermal energy, enabling the connection and reconstruction of tissues via the denaturation and crosslinking of proteins. However, the high temperatures generated in this process often lead to excessive thermal damage to tissues, thereby adversely impacting cellular activity and impeding tissue repair in practical applications. In this study, we developed a polyacrylamide/alginate (PAAm/Alg) hydrogel with high ionic conductivity (16.8 ± 1.2 S/m) achieved by introducing Ca²⁺ for the purpose of reducing thermal damage in RF tissue welding. The PAAm/Alg-Ca²⁺_0.5M hydrogel possessed excellent mechanical properties with a stress of 315.6 ± 14.1 kPa and an elongation of 382.7 ± 89.0%. Additionally, the hydrogel exhibited a high water content (83.7 ± 0.3%) and excellent stability of swelling property in water. In addition, the hydrogel extract showed good biocompatibility with no significant adverse effects on cell activity in the cytotoxicity test. At last, we conducted ex vivo experiments to investigate the effectiveness of the hydrogel as a cooling agent during RF tissue welding. The result showed that the maximum temperature was effectively reduced from 137.9 ± 4.7 to 101.8 ± 2.5 °C, while the strength of the anastomotic stoma (12.0 ± 3.2 kPa) was not affected by the intervention of this hydrogel. Histological analysis also revealed that the anastomotic structure of the tissue with hydrogel intervention was more intact than that of the control. Thus, the PAAm/Alg-Ca²⁺_0.5M hydrogel has been demonstrated to function effectively as a cooling agent, offering a new strategy for thermal damage control in RF tissue welding.