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Fe3O4-Cy5.5-trastuzumab magnetic nanoparticles for magnetic resonance / near-infrared imaging targeting HER2 in breast cancer. 用于乳腺癌 HER2 靶向磁共振/近红外成像的 Fe3O4-Cy5.5- 曲妥珠单抗磁性纳米粒子。
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-16 DOI: 10.1088/1748-605X/ad3f61
Qiangqiang Yin, Xiaolong Gao, Hao Zhang, Zhicheng Zhang, Xiaoyang Yu, Jialong He, Guangyue Shi, Liguo Hao
This study developed a probe Fe3O4-Cy5.5-trastuzumab with fluorescence and magnetic resonance imaging functions that can target breast cancer with high HER2 expression, aiming to provide a new theoretical method for the diagnosis of early breast cancer. Methods:Fe3O4-Cy5.5-trastuzumab nanoparticles were combined with Fe3O4 for T2 imaging and Cy5.5 for near-infrared imaging, and coupled with trastuzumab for HER2 targeting. We characterized the nanoparticles used transmission electron microscopy, hydration particle size, Zeta potential, UV and fourier transform infrared spectroscopy, and examined its magnetism, fluorescence, and relaxation rate related properties. CCK-8 and blood biochemistry analysis evaluated the biosafety and stability of the nanoparticles, and validated the targeting ability of Fe3O4-Cy5.5 trastuzumab nanoparticles through in vitro and in vivo cell and animal experiments. Results: Characterization results showed the successful synthesis of Fe3O4-Cy5.5-trastuzumab nanoparticles with a diameter of 93.72±6.34 nm. The nanoparticles showed a T2 relaxation rate 42.29 mM-1s-1, magnetic saturation strength of 27.58 emg/g. Laser confocal and flow cytometry uptake assay showed that the nanoparticles could effectively target HER2 expressed by breast cancer cells. As indicated by in vitro and in vivo studies, Fe3O4-Cy5.5-trastuzumab were specifically taken up and effectively aggregated to tumor regions with prominent NIRF/MR imaging properties. CCK-8, blood biochemical analysis and histological results suggested Fe3O4-Cy5.5-trastuzumab that exhibited low toxicity to major organs and good in vivo biocompatibility. The prepared Fe3O4-Cy5.5-trastuzumab exhibited excellent targeting, NIRF/MR imaging performance. It is expected to serve as a safe and effective diagnostic method that lays a theoretical basis for the effective diagnosis of of early breast cancer. Conclusion: This study successfully prepared a kind of nanoparticles with near-infrared fluorescence imaging and T2 imaging properties, which is expected to serve as a new theory and strategy for early detection of breast cancer. Keywords Breast cancer ; HER2 ;Trastuzumab ;T2 imaging properties; Near infrared fluorescence imaging; Early detection .
本研究开发了一种具有荧光和磁共振成像功能的探针Fe3O4-Cy5.5-曲妥珠单抗,可靶向HER2高表达的乳腺癌,旨在为早期乳腺癌的诊断提供一种新的理论方法。方法:Fe3O4-Cy5.5-曲妥珠单抗纳米颗粒与Fe3O4结合用于T2成像,与Cy5.5结合用于近红外成像,并与曲妥珠单抗结合用于HER2靶向。我们利用透射电子显微镜、水合粒度、Zeta 电位、紫外光谱和傅立叶变换红外光谱对纳米粒子进行了表征,并研究了其磁性、荧光和弛豫速率相关特性。CCK-8和血液生化分析评估了纳米颗粒的生物安全性和稳定性,并通过体外和体内细胞及动物实验验证了Fe3O4-Cy5.5曲妥珠单抗纳米颗粒的靶向能力。结果表征结果表明,成功合成了直径为 93.72±6.34 nm 的 Fe3O4-Cy5.5 曲妥珠单抗纳米颗粒。纳米颗粒的 T2 弛豫速率为 42.29 mM-1s-1,磁饱和强度为 27.58 emg/g。激光共聚焦和流式细胞仪摄取分析表明,该纳米颗粒能有效靶向乳腺癌细胞表达的 HER2。体外和体内研究表明,Fe3O4-Cy5.5-曲妥珠单抗被特异性摄取并有效聚集到肿瘤区域,具有显著的近红外/红外成像特性。CCK-8、血液生化分析和组织学结果表明,Fe3O4-Cy5.5-曲妥珠单抗对主要器官毒性低,体内生物相容性好。制备的 Fe3O4-Cy5.5-trastuzumab 具有良好的靶向性和近红外/红外成像性能。它有望成为一种安全有效的诊断方法,为有效诊断早期乳腺癌奠定理论基础。结论本研究成功制备了一种具有近红外荧光成像和T2成像特性的纳米颗粒,有望成为乳腺癌早期检测的新理论和新策略。关键词 乳腺癌;HER2;曲妥珠单抗;T2成像特性;近红外荧光成像;早期检测 。
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引用次数: 0
Fully synthetic, tunable poly(α-amino acids) as the base of bioinks curable by visible light. 全合成、可调聚(α-氨基酸)作为可通过可见光固化的生物墨水的基体。
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-16 DOI: 10.1088/1748-605X/ad3f62
A. Golunova, Jana Dvořáková, Nadiia Velychkivska, Beata Strachota, Aneta Dydowiczová, Jiří Trousil, V. Proks
Bioinks play a crucial role in tissue engineering, influencing mechanical and chemical properties of the printed scaffold as well as the behavior of encapsulated cells. Recently, there has been a shift from animal origin materials to their synthetic alternatives. In this context, we present here bioinks based on fully synthetic and biodegradable poly(α,L-amino acids) (PolyAA) as an alternative to animal-based gelatin methacrylate (Gel-Ma) bioinks. Additionally, we first reported the possibility of the visible light photoinitiated incorporation of the bifunctional cell adhesive RGD peptide into the PolyAA hydrogel matrix. The obtained hydrogels are shown to be cytocompatible, and their mechanical properties closely resemble those of gelatin methacrylate-based scaffolds. Moreover, combining the unique properties of PolyAA-based bioinks, the photocrosslinking strategy, and the use of droplet-based printing allows the printing of constructs with high shape fidelity and structural integrity from low-viscosity bioinks without using any sacrificial components. Overall, presented PolyAA-based materials are a promising and versatile toolbox that extends the range of bioinks for droplet bioprinting.
生物墨水在组织工程中起着至关重要的作用,它影响着打印支架的机械和化学特性以及包裹细胞的行为。最近,人们开始从动物源材料转向合成材料。在此背景下,我们介绍了基于全合成、可生物降解的聚(α,L-氨基酸)(PolyAA)的生物墨水,作为动物基甲基丙烯酸明胶(Gel-Ma)生物墨水的替代品。此外,我们首次报道了在可见光光引发下将双功能细胞粘合剂 RGD 肽掺入 PolyAA 水凝胶基质的可能性。结果表明,所获得的水凝胶具有细胞相容性,其机械性能与基于明胶甲基丙烯酸酯的支架非常相似。此外,结合聚AA 基生物墨水的独特性能、光交联策略和液滴打印技术的使用,可以在不使用任何牺牲成分的情况下,利用低粘度生物墨水打印出具有高形状保真度和结构完整性的构建体。总之,所介绍的基于 PolyAA 的材料是一种前景广阔的多功能工具箱,可扩展液滴生物打印的生物墨水范围。
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引用次数: 0
3D printing of an artificial intelligence-generated patient-specific coronary artery segmentation in a support bath. 在支撑槽中进行人工智能生成的患者特异性冠状动脉分段 3D 打印。
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-16 DOI: 10.1088/1748-605X/ad3f60
Serkan Sokmen, Soner Çakmak, Ilkay Oksuz
Accurate segmentation of coronary artery tree and personalised 3D printing from medical images is essential for CAD diagnosis and treatment. The current literature on 3D printing relies solely on generic models created with different software or 3D coronary artery models manually segmented from medical images. Moreover, there are not many studies examining the bioprintability of a 3D model generated by artificial intelligence (AI) segmentation for complex and branched structures. In this study, deep learning algorithms with transfer learning have been employed for accurate segmentation of the coronary artery tree from medical images to generate printable segmentations. We propose a combination of deep learning and 3D printing, which accurately segments and prints complex vascular patterns in coronary arteries. Then, we performed the 3D printing of the AI-generated coronary artery segmentation for the fabrication of bifurcated hollow vascular structure. Our results indicate improved performance of segmentation with the aid of transfer learning with a Dice overlap score of 0.86 on a test set of 10 CTA images. Then, bifurcated regions from 3D models were printed into the Pluronic F-127 support bath using alginate+glucomannan hydrogel. We successfully fabricated the bifurcated coronary artery structures with high length and wall thickness accuracy, however, the outer diameters of the vessels and length of the bifurcation point differ from the 3D models. The extrusion of unnecessary material, primarily observed when the nozzle moves from left to the right vessel during 3D printing, can be mitigated by adjusting the nozzle speed. Moreover, the shape accuracy can also be improved by designing a multi-axis printhead that can change the printing angle in three dimensions. Thus, this study demonstrates the potential of the use of AI-segmented 3D models in the 3D printing of coronary artery structures and, when further improved, can be used for the fabrication of patient-specific vascular implants.
准确分割冠状动脉树并根据医学图像进行个性化三维打印对 CAD 诊断和治疗至关重要。目前有关三维打印的文献仅依赖于使用不同软件创建的通用模型或从医学影像中手动分割的三维冠状动脉模型。此外,对人工智能(AI)分割生成的复杂和分支结构三维模型的生物打印性进行研究的并不多。在这项研究中,我们采用了带有迁移学习的深度学习算法,从医学图像中准确分割冠状动脉树,生成可打印的分割图像。我们提出了一种深度学习与三维打印相结合的方法,可以精确地分割并打印出冠状动脉中复杂的血管形态。然后,我们对人工智能生成的冠状动脉分割进行了三维打印,用于制造分叉的空心血管结构。我们的结果表明,借助迁移学习,在 10 张 CTA 图像的测试集上,分割性能得到了提高,Dice 重叠得分达到了 0.86。然后,使用藻酸盐+葡甘露聚糖水凝胶将三维模型中的分叉区域打印到 Pluronic F-127 支撑槽中。我们成功制作出了长度和壁厚精确度较高的冠状动脉分叉结构,但血管外径和分叉点长度与三维模型存在差异。在三维打印过程中,主要是当喷嘴从左侧移到右侧血管时,会观察到不必要的材料挤出,这可以通过调整喷嘴速度来缓解。此外,还可以通过设计可在三维空间改变打印角度的多轴打印头来提高形状精度。因此,这项研究证明了在冠状动脉结构的三维打印中使用人工智能分段三维模型的潜力,如果进一步改进,还可用于制造特定患者的血管植入物。
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引用次数: 0
Surface engineered nanodiamonds: mechanistic intervention in biomedical applications for diagnosis and treatment of cancer 表面工程纳米金刚石:癌症诊断和治疗生物医学应用中的机理干预
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-15 DOI: 10.1088/1748-605x/ad3abb
Tanima Dey, Anushikha Ghosh, Arka Sanyal, Chelsea Josephine Charles, Sahas Pokharel, Lakshmi Nair, Manjari Singh, Santanu Kaity, Velayutham Ravichandiran, Kulwinder Kaur, Subhadeep Roy
In terms of biomedical tools, nanodiamonds (ND) are a more recent innovation. Their size typically ranges between 4 to 100 nm. ND are produced via a variety of methods and are known for their physical toughness, durability, and chemical stability. Studies have revealed that surface modifications and functionalization have a significant influence on the optical and electrical properties of the nanomaterial. Consequently, surface functional groups of NDs have applications in a variety of domains, including drug administration, gene delivery, immunotherapy for cancer treatment, and bio-imaging to diagnose cancer. Additionally, their biocompatibility is a critical requisite for their in vivo and in vitro interventions. This review delves into these aspects and focuses on the recent advances in surface modification strategies of NDs for various biomedical applications surrounding cancer diagnosis and treatment. Furthermore, the prognosis of its clinical translation has also been discussed.
就生物医学工具而言,纳米金刚石(ND)是一种最新的创新技术。它们的尺寸通常在 4 到 100 纳米之间。纳米金刚石的生产方法多种多样,并以其物理韧性、耐久性和化学稳定性而著称。研究表明,表面修饰和功能化对纳米材料的光学和电学特性有重大影响。因此,NDs 的表面功能基团可应用于多种领域,包括给药、基因递送、癌症治疗的免疫疗法和诊断癌症的生物成像。此外,其生物相容性也是其体内和体外干预的关键必要条件。本综述深入探讨了这些方面,并重点介绍了最近在围绕癌症诊断和治疗的各种生物医学应用的玖龙纸张表面改性策略方面取得的进展。此外,还讨论了其临床转化的前景。
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引用次数: 0
Fibroblast matrix implants - a better alternative for incisional hernia repair? 成纤维细胞基质植入物--切口疝修补术的更好选择?
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-11 DOI: 10.1088/1748-605X/ad3da4
Siufui Hendrawan, J. Lheman, Ursula Weber, Christian Eugen Oberkofler, Astheria Eryani, René Vonlanthen, Hans Ulrich Baer
The standard surgical procedure for abdominal hernia repair with conventional prosthetic mesh still results in a high recurrence rate. In the present study, we propose a Fibroblast Matrix Implant (FMI), which is a three-dimensional (3D) Poly-L-lactic acid (PLLA) scaffold coated with collagen (matrix) and seeded with fibroblasts, as an alternative mesh for hernia repair. The matrix was seeded with fibroblasts (cellularized) and treated with a Conditioned Medium (CM) of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSC). Fibroblast proliferation and function were assessed and compared between treated with CM hUC-MSC and untreated group, 24 hours after seeding onto the matrix (n=3). To study the matrices in vivo, the hernia was surgically created on male Sprague Dawley rats and repaired with four different grafts (n=3), including a commercial mesh (mesh group), a matrix without cells (cell-free group), a matrix seeded with fibroblasts (FMI group), and a matrix seeded with fibroblasts and cultured in medium treated with 1 % CM hUC-MSC (FMI-CM group). In vitro examination showed that the fibroblasts' proliferation on the matrices (treated group) did not differ significantly compared to the untreated group. CM hUC-MSC was able to promote the collagen synthesis of the fibroblasts, resulting in a higher collagen concentration compared to the untreated group. Furthermore, the in vivo study showed that the matrices allowed fibroblast growth and supported cell functionality for at least 1 month after implantation. The highest number of fibroblasts was observed in the FMI group at the 14-day endpoint, but at the 28-day endpoint, the FMI-CM group had the highest. Collagen deposition area and neovascularization at the implantation site were observed in all groups without any significant difference between the groups. FMI combined with CM hUC-MSC may serve as a better option for hernia repair, providing additional reinforcement which in turn should reduce hernia recurrence. .
使用传统假体网片进行腹腔疝修补的标准手术程序仍会导致较高的复发率。在本研究中,我们提出了一种成纤维细胞基质植入物(FMI),它是一种涂有胶原蛋白(基质)并播种有成纤维细胞的三维(3D)聚左旋乳酸(PLLA)支架,可作为疝修补术的替代网片。基质中接种了成纤维细胞(细胞化),并用人类脐带间充质干细胞(hUC-MSC)的条件培养基(CM)进行处理。在基质上播种 24 小时后,对成纤维细胞的增殖和功能进行了评估,并比较了经 CM 处理的 hUC-MSC 组和未经处理的组(n=3)。为了研究体内基质,对雄性 Sprague Dawley 大鼠进行了疝气手术,并用四种不同的移植物(n=3)进行了修复,包括商用网片(网片组)、不含细胞的基质(无细胞组)、播种了成纤维细胞的基质(FMI 组)以及播种了成纤维细胞并在经 1% CM hUC-MSC 处理的培养基中培养的基质(FMI-CM 组)。体外检查显示,与未处理组相比,成纤维细胞在基质(处理组)上的增殖没有明显差异。CM hUC-间充质干细胞能促进成纤维细胞的胶原蛋白合成,从而使胶原蛋白浓度高于未处理组。此外,体内研究表明,基质允许成纤维细胞生长,并在植入后至少 1 个月内支持细胞功能。在 14 天的终点,FMI 组的成纤维细胞数量最多,但在 28 天的终点,FMI-CM 组的成纤维细胞数量最多。所有组别都观察到了植入部位的胶原沉积面积和新生血管,组间无明显差异。FMI与CM hUC-间充质干细胞结合可作为疝修补的更好选择,提供额外的加固,从而减少疝的复发。.
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引用次数: 0
Spinning with exosomes: electrospun nanofibers for efficient targeting of stem cell-derived exosomes in tissue regeneration. 与外泌体一起旋转:电纺纳米纤维在组织再生中有效靶向干细胞衍生的外泌体。
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-09 DOI: 10.1088/1748-605X/ad3cab
Ritu Raj, Parinita Agrawal, Utkarsh Bhutani, T. Bhowmick, Arun Chandru
Electrospinning technique converts polymeric solutions into nanoscale fibers using an electric field and can be used for various biomedical and clinical applications. Extracellular vesicles (EVs) are cell-derived small lipid vesicles enriched with biological cargo (proteins and nucleic acids) potential therapeutic applications. In this review, we discuss extending the scope of electrospinning by incorporating stem cell-derived EVs, particularly exosomes, into nanofibers for their effective delivery to target tissues. The parameters used during the electrospinning of biopolymers limit the stability and functional properties of cellular products. However, with careful consideration of process requirements, these can significantly improve stability, leading to longevity, effectiveness, and sustained and localized release. Electrospun nanofibers are known to encapsulate or surface-adsorb biological payloads such as therapeutic EVs, proteins, enzymes, and nucleic acids. Small EVs, specifically exosomes, have recently attracted the attention of researchers working on regeneration and tissue engineering because of their broad distribution and enormous potential as therapeutic agents. This review focuses on current developments in nanofibers for delivering therapeutic cargo molecules, with a special emphasis on exosomes. It also suggests prospective approaches that can be adapted to safely combine these two nanoscale systems and exponentially enhance their benefits in tissue engineering, medical device coating, and drug delivery applications.
电纺丝技术利用电场将聚合物溶液转化为纳米级纤维,可用于各种生物医学和临床应用。细胞外囊泡(EVs)是源自细胞的小型脂质囊泡,富含生物货物(蛋白质和核酸),具有潜在的治疗用途。在这篇综述中,我们讨论了通过将干细胞衍生的EV(特别是外泌体)纳入纳米纤维来扩展电纺丝的范围,从而将其有效输送到目标组织。生物聚合物电纺丝过程中使用的参数限制了细胞产品的稳定性和功能特性。不过,只要仔细考虑工艺要求,这些参数就能显著提高稳定性,从而延长寿命、提高功效、实现持续和局部释放。众所周知,电纺纳米纤维可以封装或表面吸附生物载荷,如治疗性 EV、蛋白质、酶和核酸。小的 EVs,特别是外泌体,由于其广泛的分布和作为治疗剂的巨大潜力,最近吸引了从事再生和组织工程研究人员的注意。这篇综述重点介绍了目前纳米纤维在递送治疗货物分子方面的发展,并特别强调了外泌体。它还提出了一些前瞻性的方法,这些方法可以安全地将这两种纳米级系统结合起来,成倍地提高它们在组织工程、医疗设备涂层和药物输送应用中的效益。
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引用次数: 0
All-aqueous droplets-templated tailorable core-shell alginate microspheres for constructing vascularized intestinal mucosa in vitro models. 用于构建血管化肠粘膜体外模型的全水滴-可定制核壳藻酸盐微球。
IF 4 3区 医学 Q2 Engineering Pub Date : 2024-04-04 DOI: 10.1088/1748-605X/ad3abc
Xin Hao, Ting Du, Feng Yang, Yilan Wang, Huatao He, Menghan Yang, Meiying Hong, Guanxiong Wang, Deqing Huang, Yaolei Wang
Recently, in vitro models of intestinal mucosa have become important tools for drug screening and studying the physiology and pathology of the intestine. These models enable the examination of cellular behavior in diseased states or in reaction to alterations in the microenvironment, potentially serving as alternatives to animal models. One of the major challenges in constructing physiologically relevant in vitro models of intestinal mucosa is the creation of three-dimensional (3D) microstructures that accurately mimic the integration of intestinal epithelium and vascularized stroma. Here, core-shell alginate (Alg) microspheres were generated to create the compartmentalized extracellular matrix (ECM) microenvironment needed to simulate the epithelial and vascularized stromal compartments of the intestinal mucosa. We demonstrated that NIH-3T3 and human umbilical vein endothelial cells (HUVECs) embedded in the core of the microspheres can proliferate and develop a vascular network, while human colorectal adenocarcinoma cells (Caco-2) can form an epithelial monolayer in the shell. Compared to Caco-2 monolayer encapsulated within the shell, the presence of the vascularized stroma enhances their proliferation and functionality. As such, our core-shell Alg microspheres provide a valuable method for generating in vitro models of vascularized intestinal mucosa with epithelial and vascularized stroma arranged in a spatially relevant manner and demonstrating near-physiological functionality.
最近,肠粘膜体外模型已成为药物筛选和研究肠道生理与病理的重要工具。通过这些模型,可以研究细胞在疾病状态下的行为或对微环境改变的反应,有可能成为动物模型的替代品。构建与生理相关的体外肠粘膜模型的主要挑战之一是创建三维(3D)微结构,以准确模拟肠上皮和血管基质的整合。在这里,我们生成了核壳藻酸盐(Alg)微球,以创建模拟肠粘膜上皮和血管基质所需的分区细胞外基质(ECM)微环境。我们证明,嵌入微球核心的 NIH-3T3 和人脐静脉内皮细胞(HUVEC)可以增殖并形成血管网络,而人结肠直肠腺癌细胞(Caco-2)可以在外壳中形成上皮单层。与包裹在外壳内的 Caco-2 单层细胞相比,血管基质的存在能增强它们的增殖和功能。因此,我们的核壳 Alg 微球为体外生成血管化肠粘膜模型提供了一种有价值的方法,这种模型的上皮和血管化基质以空间相关的方式排列,并显示出接近生理的功能。
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引用次数: 0
Design of novel bioadhesive chitosan film loaded with bimetallic gold-silver nanoparticles for antibiofilm and wound healing activity 负载双金属金-银纳米粒子的新型生物粘附壳聚糖膜的设计及其抗生物膜和伤口愈合活性
IF 4 3区 医学 Q2 Engineering Pub Date : 2023-02-02 DOI: 10.1088/1748-605X/acb89b
C. Singh, A. K. Mehata, V. ., P. Tiwari, Aseem Setia, Ankit Malik, Sanjeev K Singh, Rashmi M. Tilak, M. S. Muthu
Microbial infections and antibiotic resistance are among the leading causes of morbidity and mortality worldwide. The bimetallic chitosan (CS)-capped gold-silver nanoparticles (CS-AuAg-NPs) were prepared by the seeded growth synthesis technique. The nanoparticles were optimized for particle size (PS), zeta potential (ZP) and antibacterial activity by Box–Behnken design at three levels and three factors. The developed CS-AuAg-NPs were polydispersed with mean hydrodynamic PS in the range of 55 – 289 nm and ZP ranges from +8.53 mV to +38.6 mV. The optimized CS-AuAg-NPs found to have a minimum inhibitory concentration and minimal bactericidal concentration of 1.625 ± 0.68 and 3.25 ± 0.74 µg ml−1 towards multidrug resistant (MDR) Staphylococcus aureus ATCC 25923 (MDR AT) and 3.25 ± 0.93 and 3.25 ± 0.86 µg ml−1 towards MDR S. aureus clinical isolate MDR1695 (MDR CI) strain, respectively. The CS-AuAg-NPs were much more effective against MDR AT and MDR CI compared to clindamycin standard. The live/dead assay of clinical isolates strain demonstrated significant reduction of bacterial cells ∼67.52 folds compared to control group in 12 h. The hemolysis study suggested that CS-AuAg-NPs were non-hemolytic and safer for application in the wound. Furthermore, CS-AuAg-NPs were distributed in the CS film, which showed 87% wound recovery after 7 d in mice model. Hence, we concluded that CS-AuAg-NPs was safer and more effective against MDR bacteria and capable of skin regeneration in the infected wound.
微生物感染和抗生素耐药性是全世界发病率和死亡率的主要原因之一。采用种子生长合成技术制备了双金属壳聚糖(CS)包覆的金-银纳米粒子(CS- auag - nps)。采用Box-Behnken设计,对纳米颗粒粒径(PS)、ζ电位(ZP)和抗菌活性进行了3个层次、3个因素的优化。制备的CS-AuAg-NPs是多分散的,平均水动力PS范围为55 ~ 289 nm, ZP范围为+8.53 mV ~ +38.6 mV。优化后的CS-AuAg-NPs对耐多药金黄色葡萄球菌ATCC 25923 (MDR AT)的最小抑菌浓度为1.625±0.68µg ml - 1,对耐多药金黄色葡萄球菌临床分离株MDR1695 (MDR CI)的最小抑菌浓度为3.25±0.93µg ml - 1,对耐多药金黄色葡萄球菌ATCC 25923 (MDR AT)的最小杀菌浓度为3.25±0.74µg ml - 1。与克林霉素标准相比,CS-AuAg-NPs对耐多药AT和耐多药CI更有效。临床分离菌株的活/死试验显示,与对照组相比,12小时内细菌细胞显著减少约67.52倍。溶血研究表明,CS-AuAg-NPs无溶血作用,应用于伤口更安全。此外,CS- auag - nps分布在CS膜中,在小鼠模型中,7 d后伤口恢复率为87%。因此,我们得出结论,CS-AuAg-NPs对耐多药细菌更安全,更有效,并且能够在感染伤口中进行皮肤再生。
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引用次数: 1
3D printed bioresorbable scaffolds for articular cartilage tissue engineering: a comparative study between neat polycaprolactone (PCL) and poly(lactide-b-ethylene glycol) (PLA-PEG) block copolymer 3D打印关节软骨组织工程生物可吸收支架:纯聚己内酯(PCL)与聚乳酸-b-乙二醇(PLA-PEG)嵌段共聚物的比较研究
IF 4 3区 医学 Q2 Engineering Pub Date : 2022-06-14 DOI: 10.1088/1748-605X/ac78b7
Uzuri Urtaza, O. Guaresti, Izar Gorroñogoitia, Ana Zubiarrain-Laserna, Emma Muiños‐López, Froilán Granero-Moltó, JM Lamo de Espinosa, T. López-Martínez, M. Mazo, F. Prósper, A. Zaldua, J. Anakabe
This work identifies and describes different material-scaffold geometry combinations for cartilage tissue engineering (CTE). Previously reported potentially interesting scaffold geometries were tuned and printed using bioresorbable polycaprolactone and poly(lactide-b-ethylene) block copolymer. Medical grades of both polymers were 3D printed with fused filament fabrication technology within an ISO 7 classified cleanroom. Resulting scaffolds were then optically, mechanically and biologically tested. Results indicated that a few material-scaffold geometry combinations present potential for excellent cell viability as well as for an enhance of the chondrogenic properties of the cells, hence suggesting their suitability for CTE applications.
这项工作确定并描述了软骨组织工程(CTE)的不同材料-支架几何组合。先前报道的可能有趣的支架几何形状是使用生物可吸收的聚己内酯和聚(丙交酯-乙乙烯)嵌段共聚物进行调整和打印的。这两种聚合物的医用级都是在ISO 7级洁净室中使用熔丝制造技术进行3D打印的。然后对得到的支架进行光学、机械和生物学测试。结果表明,一些材料-支架的几何组合具有优异的细胞活力和增强细胞软骨性质的潜力,因此表明它们适用于CTE应用。
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引用次数: 2
A tunable gelatin-hyaluronan dialdehyde/methacryloyl gelatin interpenetrating polymer network hydrogel for additive tissue manufacturing 用于添加剂组织制造的可调明胶-透明质酸双醛/甲基丙烯酰明胶互穿聚合物网络水凝胶
IF 4 3区 医学 Q2 Engineering Pub Date : 2022-06-14 DOI: 10.1088/1748-605X/ac78b8
R. Anand, Mehdi Salar Amoli, An-Sofie Huysecom, P. Amorim, Hannah Agten, L. Geris, V. Bloemen
Methacryloyl gelatin (GelMA) is a versatile material for bioprinting because of its tunable physical properties and inherent bioactivity. Bioprinting of GelMA is often met with challenges such as lower viscosity of GelMA inks due to higher methacryloyl substitution and longer physical gelation time at room temperature. In this study, a tunable interpenetrating polymer network (IPN) hydrogel was prepared from gelatin-hyaluronan dialdehyde (Gel-HDA) Schiff’s polymer, and 100% methacrylamide substituted GelMA for biofabrication through extrusion based bioprinting. Temperature sweep rheology measurements show a higher sol-gel transition temperature for IPN (30 °C) compared to gold standard GelMA (27 °C). Furthermore, to determine the tunability of the IPN hydrogel, several IPN samples were prepared by combining different ratios of Gel-HDA and GelMA achieving a compressive modulus ranging from 20.6 ± 2.48 KPa to 116.7 ± 14.80 KPa. Our results showed that the mechanical properties and printability at room temperature could be tuned by adjusting the ratios of GelMA and Gel-HDA. To evaluate cell response to the material, MC3T3-E1 mouse pre-osteoblast cells were embedded in hydrogels and 3D-printed, demonstrating excellent cell viability and proliferation after 10 d of 3D in vitro culture, making the IPN an interesting bioink for the fabrication of 3D constructs for tissue engineering applications.
甲基丙烯酰明胶(GelMA)具有可调的物理性能和固有的生物活性,是一种多用途的生物打印材料。GelMA的生物打印经常遇到挑战,例如由于较高的甲基丙烯酰基取代度和室温下较长的物理凝胶化时间,GelMA油墨的粘度较低。在本研究中,以明胶-透明质酸二醛(Gel HDA)Schiff’s聚合物和100%甲基丙烯酰胺取代的GelMA为原料,通过挤出生物打印制备了可调互穿聚合物网络(IPN)水凝胶。温度扫描流变学测量显示,与金标准GelMA(27°C)相比,IPN(30°C)的溶胶-凝胶转变温度更高。此外,为了确定IPN水凝胶的可调性,通过组合不同比例的Gel HDA和GelMA制备了几个IPN样品,获得了20.6±2.48 KPa至116.7±14.80 KPa的压缩模量。我们的结果表明,可以通过调节GelMA和Gel-HDA的比例来调节室温下的机械性能和可印刷性。为了评估细胞对该材料的反应,将MC3T3-E1小鼠前成骨细胞包埋在水凝胶中并进行3D打印,在3D体外培养10天后显示出优异的细胞活力和增殖,使IPN成为制造组织工程应用的3D构建体的有趣的生物墨水。
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引用次数: 4
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Biomedical materials
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