首页 > 最新文献

BMC Genomics最新文献

英文 中文
Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters. 睾丸和附睾的全转录组测序揭示了与公鸡精子发育有关的基因。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s12864-024-10836-8
Shihao Guo, Bailin Cong, Liyang Zhu, Yao Zhang, Ying Yang, Xiaolong Qi, Xiangguo Wang, Longfei Xiao, Cheng Long, Yaxi Xu, Xihui Sheng

Background: Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development.

Results: In this study, we established transcriptome profiles of lncRNAs, miRNAs, and mRNAs in 3 testis tissues and 3 epididymis tissues from "Jing Hong No.1" roosters at 24, 35, and 64 weeks of age. Using the data, we conducted whole transcriptome analysis and constructed a ceRNA network. We detected 10 differentially expressed mRNAs (DEmRNAs), 33 differentially expressed lncRNAs (DElncRNAs), and 10 differentially expressed miRNAs (DEmiRNAs) in the testis, as well as 149 DEmRNAs, 12 DElncRNAs, and 10 DEmiRNAs in the epididymis. These genes were found to be involved in cell differentiation and development, as well as various signaling pathways such as GnRH, MAPK, TGF-β, mTOR, VEGF, and calcium ion pathways. Subsequently, we constructed two competing endogenous RNA (ceRNA) networks comprising DEmRNAs, DElncRNAs, and DEmiRNAs. Furthermore, we identified four crucial lncRNA-mRNA-miRNA interactions that govern specific biological processes in the chicken reproductive system: MSTRG.2423.1-gga-miR-1563-PPP3CA and MSTRG.10064.2-gga-miR-32-5p-GPR12 regulating sperm motility in the testis; MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 governing immunomodulation in the epididymis; and MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 controlling epididymal sperm maturation and motility.

Conclusions: Whole transcriptome sequencing of chicken testis and epididymis screened several key genes and ceRNA regulatory networks, which may be involved in the regulation of epididymal immunity, spermatogenesis and sperm viability through the pathways of MAPK, TGF-β, mTOR, and calcium ion. These findings contribute to our comprehensive understanding of the intricate molecular processes underlying rooster spermatogenesis, maturation and motility.

背景:鸡作为全球鸡蛋和家禽的主要来源发挥着至关重要的作用,而公鸡精液的质量对家禽的繁殖效率有着重大影响。因此,了解精子发育的调控机制势在必行:在这项研究中,我们建立了 "京红1号 "公鸡在24周龄、35周龄和64周龄时3个睾丸组织和3个附睾组织中lncRNA、miRNA和mRNA的转录组图谱。利用这些数据,我们进行了全转录组分析,并构建了一个 ceRNA 网络。我们在睾丸中检测到了10个差异表达的mRNA(DEmRNA)、33个差异表达的lncRNA(DElncRNA)和10个差异表达的miRNA(DEmiRNA),在附睾中检测到了149个DEmRNA、12个DElncRNA和10个DEmiRNA。这些基因被发现参与了细胞分化和发育,以及各种信号通路,如 GnRH、MAPK、TGF-β、mTOR、VEGF 和钙离子通路。随后,我们构建了由 DEmRNA、DElncRNA 和 DEmiRNA 组成的两个竞争性内源性 RNA(ceRNA)网络。此外,我们还发现了四种关键的lncRNA-mRNA-miRNA相互作用,它们支配着鸡生殖系统的特定生物过程:MSTRG.2423.1-gga-miR-1563-PPP3CA和MSTRG.10064.2-gga-miR-32-5p-GPR12调节睾丸中的精子活力;MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1调节附睾中的免疫调节;MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1控制附睾精子的成熟和活力:鸡睾丸和附睾的全转录组测序筛选出了几个关键基因和ceRNA调控网络,它们可能通过MAPK、TGF-β、mTOR和钙离子等途径参与附睾免疫、精子发生和精子活力的调控。这些发现有助于我们全面了解公鸡精子发生、成熟和运动的复杂分子过程。
{"title":"Whole transcriptome sequencing of testis and epididymis reveals genes associated with sperm development in roosters.","authors":"Shihao Guo, Bailin Cong, Liyang Zhu, Yao Zhang, Ying Yang, Xiaolong Qi, Xiangguo Wang, Longfei Xiao, Cheng Long, Yaxi Xu, Xihui Sheng","doi":"10.1186/s12864-024-10836-8","DOIUrl":"10.1186/s12864-024-10836-8","url":null,"abstract":"<p><strong>Background: </strong>Chickens play a crucial role as the primary global source of eggs and poultry, and the quality of rooster semen significantly impacts poultry reproductive efficiency. Therefore, it is imperative to comprehend the regulatory mechanisms underlying sperm development.</p><p><strong>Results: </strong>In this study, we established transcriptome profiles of lncRNAs, miRNAs, and mRNAs in 3 testis tissues and 3 epididymis tissues from \"Jing Hong No.1\" roosters at 24, 35, and 64 weeks of age. Using the data, we conducted whole transcriptome analysis and constructed a ceRNA network. We detected 10 differentially expressed mRNAs (DEmRNAs), 33 differentially expressed lncRNAs (DElncRNAs), and 10 differentially expressed miRNAs (DEmiRNAs) in the testis, as well as 149 DEmRNAs, 12 DElncRNAs, and 10 DEmiRNAs in the epididymis. These genes were found to be involved in cell differentiation and development, as well as various signaling pathways such as GnRH, MAPK, TGF-β, mTOR, VEGF, and calcium ion pathways. Subsequently, we constructed two competing endogenous RNA (ceRNA) networks comprising DEmRNAs, DElncRNAs, and DEmiRNAs. Furthermore, we identified four crucial lncRNA-mRNA-miRNA interactions that govern specific biological processes in the chicken reproductive system: MSTRG.2423.1-gga-miR-1563-PPP3CA and MSTRG.10064.2-gga-miR-32-5p-GPR12 regulating sperm motility in the testis; MSTRG.152556.1-gga-miR-9-3p-GREM1/THYN1 governing immunomodulation in the epididymis; and MSTRG.124708.1-gga-miR-375-NDUFB9/YBX1 controlling epididymal sperm maturation and motility.</p><p><strong>Conclusions: </strong>Whole transcriptome sequencing of chicken testis and epididymis screened several key genes and ceRNA regulatory networks, which may be involved in the regulation of epididymal immunity, spermatogenesis and sperm viability through the pathways of MAPK, TGF-β, mTOR, and calcium ion. These findings contribute to our comprehensive understanding of the intricate molecular processes underlying rooster spermatogenesis, maturation and motility.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased DNMT1 acetylation leads to global DNA methylation suppression in follicular granulosa cells during reproductive aging in mammals. 在哺乳动物生殖衰老过程中,DNMT1 乙酰化的增加导致卵泡颗粒细胞中 DNA 甲基化的全面抑制。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s12864-024-10957-0
Shunran Zhao, Haoliang Cui, Xiaohuan Fang, Wei Xia, Chenyu Tao, Junjie Li

With increasing age, the reproductive performance of women and female animals declines. However, the molecular mechanisms underlying ovarian aging and age-related fertility decline remain unclear. Granulosa cells (GCs) are suspected to play an important role in reproductive aging, and their proliferation, apoptosis, and steroid hormone secretion are used to determine the fate of follicles and ovarian function. First, we found that the proliferative ability of GCs from the old mouse group (10-month-old) decreased compared with that from the young mouse group (6-week-old), and cell cycle arrest occurred in old mice. To investigate changes in protein modification, we compared the levels of protein acetylation in GCs from young and old mice. We found that the K1118, K1120, K1122, and K1124 sites of DNA methyltransferase 1 (DNMT1) were increasingly acetylated with age, resulting in a decrease in DNMT1 protein expression. Therefore, we performed whole-genome methylation sequencing of GCs in the two groups and found that the CG methylation levels in the old group were lower than those in the young group. Furthermore, the inhibition of DNMT1 expression in GCs resulted in cell cycle arrest. This study revealed the dynamics and importance of protein acetylation and DNA methylation in GCs during reproductive aging. The findings provide a theoretical basis for studying the mechanism of reproductive aging in mammals.

随着年龄的增长,女性和雌性动物的生殖能力会下降。然而,卵巢衰老和与年龄相关的生育能力下降的分子机制仍不清楚。人们怀疑颗粒细胞(GCs)在生殖衰老中扮演重要角色,它们的增殖、凋亡和类固醇激素分泌被用来决定卵泡和卵巢功能的命运。首先,我们发现老龄小鼠组(10 个月大)的 GCs 增殖能力比年轻小鼠组(6 周大)的 GCs 减低,而且老龄小鼠的细胞周期发生停滞。为了研究蛋白质修饰的变化,我们比较了年轻小鼠和老年小鼠 GCs 中蛋白质乙酰化的水平。我们发现,随着年龄的增长,DNA甲基转移酶1(DNMT1)的K1118、K1120、K1122和K1124位点的乙酰化程度越来越高,导致DNMT1蛋白表达量减少。因此,我们对两组患者的 GC 进行了全基因组甲基化测序,发现老年组的 CG 甲基化水平低于年轻组。此外,抑制 GC 中 DNMT1 的表达会导致细胞周期停滞。这项研究揭示了生殖衰老过程中 GC 中蛋白质乙酰化和 DNA 甲基化的动态变化及其重要性。这些发现为研究哺乳动物生殖衰老的机制提供了理论依据。
{"title":"Increased DNMT1 acetylation leads to global DNA methylation suppression in follicular granulosa cells during reproductive aging in mammals.","authors":"Shunran Zhao, Haoliang Cui, Xiaohuan Fang, Wei Xia, Chenyu Tao, Junjie Li","doi":"10.1186/s12864-024-10957-0","DOIUrl":"10.1186/s12864-024-10957-0","url":null,"abstract":"<p><p>With increasing age, the reproductive performance of women and female animals declines. However, the molecular mechanisms underlying ovarian aging and age-related fertility decline remain unclear. Granulosa cells (GCs) are suspected to play an important role in reproductive aging, and their proliferation, apoptosis, and steroid hormone secretion are used to determine the fate of follicles and ovarian function. First, we found that the proliferative ability of GCs from the old mouse group (10-month-old) decreased compared with that from the young mouse group (6-week-old), and cell cycle arrest occurred in old mice. To investigate changes in protein modification, we compared the levels of protein acetylation in GCs from young and old mice. We found that the K1118, K1120, K1122, and K1124 sites of DNA methyltransferase 1 (DNMT1) were increasingly acetylated with age, resulting in a decrease in DNMT1 protein expression. Therefore, we performed whole-genome methylation sequencing of GCs in the two groups and found that the CG methylation levels in the old group were lower than those in the young group. Furthermore, the inhibition of DNMT1 expression in GCs resulted in cell cycle arrest. This study revealed the dynamics and importance of protein acetylation and DNA methylation in GCs during reproductive aging. The findings provide a theoretical basis for studying the mechanism of reproductive aging in mammals.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of cold stress on the blood-brain barrier in Plectropomus leopardus. 冷应激对豹纹鹦鹉血脑屏障的影响
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s12864-024-10943-6
Yilan Guo, Cun Wei, Hui Ding, Peiyu Li, Yurui Gao, Kangning Zhong, Zhenmin Bao, Zhe Qu, Bo Wang, Jingjie Hu

Background: The leopard coral grouper (Plectropomus leopardus) is a commercially valuable tropical marine fish species known to be sensitive to low temperatures. A comprehensive understanding of the molecular mechanisms governing its response to acute cold stress is of great importance. However, there is a relative scarcity of fundamental research on low-temperature tolerance in the leopard coral grouper.

Methods: In this study, a cooling and rewarming experiment was conducted on 6-month-old leopard coral groupers. Within 24 h, we decreased the ambient temperature from 25 °C to 13 °C and subsequently allowed it to naturally return to 25 °C. During this process, a comprehensive investigation of serum hormone levels, enzyme activity, and brain transcriptome analysis was performed.

Results: P. leopardus displayed a noticeable adaptive response to the initial temperature decrease by temporarily reducing its life activities. Our transcriptome analysis revealed that the differentially expressed genes (DEGs) were primarily concentrated in crucial pathways including the blood-brain barrier (BBB), inflammatory response, and coagulation cascade. In situ hybridization of claudin 15a (cldn15a), a key gene for BBB maintaining, further confirmed that exposure to low temperatures led to the disruption of the blood-brain barrier and stimulated a pronounced inflammatory reaction within the brain. Upon rewarming, there was a recovery of BBB integrity accompanied by the persistence of inflammation within the brain tissue.

Conclusions: Our study reveals the complex interactions between blood-brain barrier function, inflammation, and recovery in P. leopardus during short-term temperature drops and rewarming. These findings provide valuable insights into the physiological responses of this species under cold stress conditions.

背景:豹纹珊瑚石斑鱼(Plectropomus leopardus)是一种具有商业价值的热带海洋鱼类,对低温非常敏感。全面了解其对急性低温应激反应的分子机制非常重要。然而,有关豹纹石斑鱼耐低温能力的基础研究相对较少:本研究对 6 个月大的豹纹珊瑚石斑鱼进行了降温和复温实验。在24小时内,我们将环境温度从25 °C降至13 °C,然后让其自然恢复到25 °C。在此过程中,我们对血清激素水平、酶活性和脑转录组分析进行了全面调查:结果:豹纹鲤通过暂时减少生命活动,对初始温度降低表现出明显的适应性反应。我们的转录组分析表明,差异表达基因(DEGs)主要集中在血脑屏障(BBB)、炎症反应和凝血级联等关键通路。维持血脑屏障的关键基因克劳丁 15a(cldn15a)的原位杂交进一步证实,暴露于低温会导致血脑屏障破坏,并刺激脑内出现明显的炎症反应。回温后,血脑屏障的完整性得到恢复,但脑组织内的炎症却持续存在:我们的研究揭示了豹斑蛙在短期降温和复温过程中血脑屏障功能、炎症和恢复之间复杂的相互作用。这些发现为了解该物种在冷应激条件下的生理反应提供了宝贵的见解。
{"title":"Effects of cold stress on the blood-brain barrier in Plectropomus leopardus.","authors":"Yilan Guo, Cun Wei, Hui Ding, Peiyu Li, Yurui Gao, Kangning Zhong, Zhenmin Bao, Zhe Qu, Bo Wang, Jingjie Hu","doi":"10.1186/s12864-024-10943-6","DOIUrl":"10.1186/s12864-024-10943-6","url":null,"abstract":"<p><strong>Background: </strong>The leopard coral grouper (Plectropomus leopardus) is a commercially valuable tropical marine fish species known to be sensitive to low temperatures. A comprehensive understanding of the molecular mechanisms governing its response to acute cold stress is of great importance. However, there is a relative scarcity of fundamental research on low-temperature tolerance in the leopard coral grouper.</p><p><strong>Methods: </strong>In this study, a cooling and rewarming experiment was conducted on 6-month-old leopard coral groupers. Within 24 h, we decreased the ambient temperature from 25 °C to 13 °C and subsequently allowed it to naturally return to 25 °C. During this process, a comprehensive investigation of serum hormone levels, enzyme activity, and brain transcriptome analysis was performed.</p><p><strong>Results: </strong>P. leopardus displayed a noticeable adaptive response to the initial temperature decrease by temporarily reducing its life activities. Our transcriptome analysis revealed that the differentially expressed genes (DEGs) were primarily concentrated in crucial pathways including the blood-brain barrier (BBB), inflammatory response, and coagulation cascade. In situ hybridization of claudin 15a (cldn15a), a key gene for BBB maintaining, further confirmed that exposure to low temperatures led to the disruption of the blood-brain barrier and stimulated a pronounced inflammatory reaction within the brain. Upon rewarming, there was a recovery of BBB integrity accompanied by the persistence of inflammation within the brain tissue.</p><p><strong>Conclusions: </strong>Our study reveals the complex interactions between blood-brain barrier function, inflammation, and recovery in P. leopardus during short-term temperature drops and rewarming. These findings provide valuable insights into the physiological responses of this species under cold stress conditions.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Streptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes. 人类角质细胞宿主细胞进入环境中的犬链球菌转录组学变化
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-04 DOI: 10.1186/s12864-024-10974-z
Haruno Yoshida, Mieko Goto, Yuzo Tsuyuki, Jae-Seok Kim, Takashi Takahashi

Background: Streptococcus canis is a commensal bacterium in companion animals. This microorganism can infect humans who have been in deep contact with or bitten by pet dogs, suggesting that the skin/soft tissue is one of infection entry sites. To understand pathological process in human cells, we aimed to determine S. canis transcriptomic changes in invasive environments of human keratinocytes.

Methods: We selected one isolate from candidates with whole-genome sequences, based on re-obtained cell invasion ability (CIA) data into human keratinocytes along with bacterial cytotoxicity. RNA-sequencing was conducted for the samples at baselines and 2 h/5 hr post-inoculation using NovaSeq 6000. Global/differential gene expression analyses [principal component analysis (PCA)/k-means clustering analysis/differentially expressed gene (DEG) analyses] were performed. We classified DEGs into their functional categories. To validate transcriptomic results, we did quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays.

Results: FU1 isolate was selected from seven candidates, based on re-obtained CIA data with less cytotoxicity. Total read bases of 6.17-9.02 Gbp were obtained by RNA-sequencing. PCA and k-means clustering analysis indicated clustering according to their inoculation times. Volcano plots and Venn diagrams revealed that S. canis invasion into keratinocytes produced altered distributions of many genes. Gene ontology enrichment analysis showed most of the gene expressions were downregulated. DEG functional analysis showed the downregulated DEGs belonging to energy production and conversion/carbohydrate transport and metabolism/amino acid transport and metabolism/nucleotide transport and metabolism, with the upregulated DEGs belonging to transcription. qRT-PCR assays for downregulated/upregulated expressions of four genes (pgk-slo/opuAA-kdpB) validated transcriptomic results.

Conclusion: Our observations suggest that S. canis can downregulate its metabolism-associated gene expressions in human keratinocyte environments. The observed gene expression changes can imply the latent infection in human cells. Further investigation is needed to elucidate the underlying mechanisms for the latent infection.

背景:犬链球菌是伴侣动物的共生细菌。这种微生物可感染与宠物狗有深度接触或被宠物狗咬伤的人类,这表明皮肤/软组织是感染的入口之一。为了了解人体细胞的病理过程,我们旨在确定 S. canis 转录组在人角质细胞侵入环境中的变化:方法:我们根据重新获得的人类角朊细胞细胞侵袭能力(CIA)数据和细菌细胞毒性,从具有全基因组序列的候选分离株中选择了一个。使用 NovaSeq 6000 对基线和接种后 2 小时/5 小时的样本进行了 RNA 测序。进行了全局/差异基因表达分析[主成分分析(PCA)/k-均值聚类分析/差异表达基因(DEG)分析]。我们对 DEG 进行了功能分类。为了验证转录组的结果,我们进行了定量反转录聚合酶链反应(qRT-PCR)检测:结果:根据重新获得的细胞毒性较低的 CIA 数据,我们从七个候选者中选出了 FU1 分离物。通过 RNA 测序获得的总读数碱基为 6.17-9.02 Gbp。PCA 和 k-means 聚类分析显示,根据接种时间的不同,这些菌株有不同的聚类。火山图和维恩图显示,犬小孢子菌侵入角朊细胞会改变许多基因的分布。基因本体富集分析表明,大多数基因表达下调。对四个基因(pgk-slo/opuAA-kdpB)表达下调/上调的 qRT-PCR 检测验证了转录组学结果:我们的观察结果表明,犬沙门氏菌可在人类角朊细胞环境中下调其代谢相关基因的表达。观察到的基因表达变化可能意味着人类细胞中的潜伏感染。需要进一步研究以阐明潜伏感染的潜在机制。
{"title":"Streptococcus canis transcriptomic modifications in host cell entry environments of human keratinocytes.","authors":"Haruno Yoshida, Mieko Goto, Yuzo Tsuyuki, Jae-Seok Kim, Takashi Takahashi","doi":"10.1186/s12864-024-10974-z","DOIUrl":"10.1186/s12864-024-10974-z","url":null,"abstract":"<p><strong>Background: </strong>Streptococcus canis is a commensal bacterium in companion animals. This microorganism can infect humans who have been in deep contact with or bitten by pet dogs, suggesting that the skin/soft tissue is one of infection entry sites. To understand pathological process in human cells, we aimed to determine S. canis transcriptomic changes in invasive environments of human keratinocytes.</p><p><strong>Methods: </strong>We selected one isolate from candidates with whole-genome sequences, based on re-obtained cell invasion ability (CIA) data into human keratinocytes along with bacterial cytotoxicity. RNA-sequencing was conducted for the samples at baselines and 2 h/5 hr post-inoculation using NovaSeq 6000. Global/differential gene expression analyses [principal component analysis (PCA)/k-means clustering analysis/differentially expressed gene (DEG) analyses] were performed. We classified DEGs into their functional categories. To validate transcriptomic results, we did quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays.</p><p><strong>Results: </strong>FU1 isolate was selected from seven candidates, based on re-obtained CIA data with less cytotoxicity. Total read bases of 6.17-9.02 Gbp were obtained by RNA-sequencing. PCA and k-means clustering analysis indicated clustering according to their inoculation times. Volcano plots and Venn diagrams revealed that S. canis invasion into keratinocytes produced altered distributions of many genes. Gene ontology enrichment analysis showed most of the gene expressions were downregulated. DEG functional analysis showed the downregulated DEGs belonging to energy production and conversion/carbohydrate transport and metabolism/amino acid transport and metabolism/nucleotide transport and metabolism, with the upregulated DEGs belonging to transcription. qRT-PCR assays for downregulated/upregulated expressions of four genes (pgk-slo/opuAA-kdpB) validated transcriptomic results.</p><p><strong>Conclusion: </strong>Our observations suggest that S. canis can downregulate its metabolism-associated gene expressions in human keratinocyte environments. The observed gene expression changes can imply the latent infection in human cells. Further investigation is needed to elucidate the underlying mechanisms for the latent infection.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11533360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systematical characterization of Rab7 gene family in Gossypium and potential functions of GhRab7B3-A gene in drought tolerance. 格桑花 Rab7 基因家族的系统特征及 GhRab7B3-A 基因在抗旱中的潜在功能。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s12864-024-10930-x
Mengyuan Yan, Zhiwei Dong, Tian Pan, Libei Li, Ziyue Zhou, Wen Li, Zhanbo Ke, Zhen Feng, Shuxun Yu

Background: Cotton serves as a primary source of natural fibers crucial for the textile industry. However, environmental elements such as drought have posed challenges to cotton cultivation, resulting in adverse impacts on both production and fiber quality. Improving cotton's resilience to drought could mitigate yield losses and foster the expansion of cotton farming. Rab7 protein, widely present in organisms, controls the degradation and recycling of cargo, and has a potential role in biotic and abiotic tolerance. However, comprehensive exploration of the Rab7 gene family in Gossypium remains scarce.

Results: Herein, we identified a total of 10, 10, 20, and 20 Rab7 genes through genome-wide analysis in Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense, respectively. Collinearity analysis unveiled the pivotal role of whole genome or segmental duplication events in the expansion of GhRab7s. Study of gene architecture, conserved protein motifs, and domains suggested the conservation of structure and function throughout evolution. Exploration of cis-regulatory elements revealed the responsiveness of GhRab7 genes to abiotic stress, corroborated by transcriptome analysis under diverse environmental stresses. Notably, the greatly induced expression of GhRab7B3-A under drought treatment prompted us to investigate its function through virus-induced gene silencing (VIGS) assays. Silencing GhRab7B3-A led to exacerbated dehydration and wilting compared with the control. Additionally, inhibition of stomatal closure, antioxidant enzyme activities and expression patterns of genes responsive to abiotic stress were observed in GhRab7B3-A silenced plants.

Conclusions: This study sheds light on Rab7 members in cotton, identifies a gene linked to drought stress, and paves the way for additional investigation of Rab7 genes associated with drought stress tolerance.

背景:棉花是对纺织业至关重要的天然纤维的主要来源。然而,干旱等环境因素给棉花种植带来了挑战,对产量和纤维质量都造成了不利影响。提高棉花的抗旱能力可以减少产量损失,促进棉花种植业的发展。Rab7 蛋白广泛存在于生物体内,控制着货物的降解和回收,在生物和非生物耐受性方面具有潜在的作用。然而,对棉花中 Rab7 基因家族的全面探索仍然很少:结果:在此,我们通过全基因组分析,在旱麻、苎麻、大花棉和芭茅棉中分别发现了 10 个、10 个、20 个和 20 个 Rab7 基因。共线性分析揭示了全基因组或片段复制事件在 GhRab7s 扩增过程中的关键作用。对基因结构、保守蛋白基序和结构域的研究表明,结构和功能在整个进化过程中保持不变。对顺式调控元件的探索揭示了 GhRab7 基因对非生物胁迫的响应性,不同环境胁迫下的转录组分析也证实了这一点。值得注意的是,在干旱处理下,GhRab7B3-A 的表达受到极大诱导,这促使我们通过病毒诱导基因沉默(VIGS)试验研究其功能。与对照组相比,沉默 GhRab7B3-A 会导致脱水和萎蔫加剧。此外,还观察到 GhRab7B3-A 沉默植物的气孔关闭、抗氧化酶活性和对非生物胁迫反应基因的表达模式受到抑制:本研究揭示了棉花中的 Rab7 成员,发现了一个与干旱胁迫相关的基因,为进一步研究与干旱胁迫耐受性相关的 Rab7 基因铺平了道路。
{"title":"Systematical characterization of Rab7 gene family in Gossypium and potential functions of GhRab7B3-A gene in drought tolerance.","authors":"Mengyuan Yan, Zhiwei Dong, Tian Pan, Libei Li, Ziyue Zhou, Wen Li, Zhanbo Ke, Zhen Feng, Shuxun Yu","doi":"10.1186/s12864-024-10930-x","DOIUrl":"10.1186/s12864-024-10930-x","url":null,"abstract":"<p><strong>Background: </strong>Cotton serves as a primary source of natural fibers crucial for the textile industry. However, environmental elements such as drought have posed challenges to cotton cultivation, resulting in adverse impacts on both production and fiber quality. Improving cotton's resilience to drought could mitigate yield losses and foster the expansion of cotton farming. Rab7 protein, widely present in organisms, controls the degradation and recycling of cargo, and has a potential role in biotic and abiotic tolerance. However, comprehensive exploration of the Rab7 gene family in Gossypium remains scarce.</p><p><strong>Results: </strong>Herein, we identified a total of 10, 10, 20, and 20 Rab7 genes through genome-wide analysis in Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense, respectively. Collinearity analysis unveiled the pivotal role of whole genome or segmental duplication events in the expansion of GhRab7s. Study of gene architecture, conserved protein motifs, and domains suggested the conservation of structure and function throughout evolution. Exploration of cis-regulatory elements revealed the responsiveness of GhRab7 genes to abiotic stress, corroborated by transcriptome analysis under diverse environmental stresses. Notably, the greatly induced expression of GhRab7B3-A under drought treatment prompted us to investigate its function through virus-induced gene silencing (VIGS) assays. Silencing GhRab7B3-A led to exacerbated dehydration and wilting compared with the control. Additionally, inhibition of stomatal closure, antioxidant enzyme activities and expression patterns of genes responsive to abiotic stress were observed in GhRab7B3-A silenced plants.</p><p><strong>Conclusions: </strong>This study sheds light on Rab7 members in cotton, identifies a gene linked to drought stress, and paves the way for additional investigation of Rab7 genes associated with drought stress tolerance.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529164/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Amphibian Genomics Consortium: advancing genomic and genetic resources for amphibian research and conservation. 两栖动物基因组学联合会:为两栖动物研究和保护推进基因组和遗传资源。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s12864-024-10899-7
Tiffany A Kosch, María Torres-Sánchez, H Christoph Liedtke, Kyle Summers, Maximina H Yun, Andrew J Crawford, Simon T Maddock, Md Sabbir Ahammed, Victor L N Araújo, Lorenzo V Bertola, Gary M Bucciarelli, Albert Carné, Céline M Carneiro, Kin O Chan, Ying Chen, Angelica Crottini, Jessica M da Silva, Robert D Denton, Carolin Dittrich, Gonçalo Espregueira Themudo, Katherine A Farquharson, Natalie J Forsdick, Edward Gilbert, Jing Che, Barbara A Katzenback, Ramachandran Kotharambath, Nicholas A Levis, Roberto Márquez, Glib Mazepa, Kevin P Mulder, Hendrik Müller, Mary J O'Connell, Pablo Orozco-terWengel, Gemma Palomar, Alice Petzold, David W Pfennig, Karin S Pfennig, Michael S Reichert, Jacques Robert, Mark D Scherz, Karen Siu-Ting, Anthony A Snead, Matthias Stöck, Adam M M Stuckert, Jennifer L Stynoski, Rebecca D Tarvin, Katharina C Wollenberg Valero

Amphibians represent a diverse group of tetrapods, marked by deep divergence times between their three systematic orders and families. Studying amphibian biology through the genomics lens increases our understanding of the features of this animal class and that of other terrestrial vertebrates. The need for amphibian genomic resources is more urgent than ever due to the increasing threats to this group. Amphibians are one of the most imperiled taxonomic groups, with approximately 41% of species threatened with extinction due to habitat loss, changes in land use patterns, disease, climate change, and their synergistic effects. Amphibian genomic resources have provided a better understanding of ontogenetic diversity, tissue regeneration, diverse life history and reproductive modes, anti-predator strategies, and resilience and adaptive responses. They also serve as essential models for studying broad genomic traits, such as evolutionary genome expansions and contractions, as they exhibit the widest range of genome sizes among all animal taxa and possess multiple mechanisms of genetic sex determination. Despite these features, genome sequencing of amphibians has significantly lagged behind that of other vertebrates, primarily due to the challenges of assembling their large, repeat-rich genomes and the relative lack of societal support. The emergence of long-read sequencing technologies, combined with advanced molecular and computational techniques that improve scaffolding and reduce computational workloads, is now making it possible to address some of these challenges. To promote and accelerate the production and use of amphibian genomics research through international coordination and collaboration, we launched the Amphibian Genomics Consortium (AGC, https://mvs.unimelb.edu.au/amphibian-genomics-consortium ) in early 2023. This burgeoning community already has more than 282 members from 41 countries. The AGC aims to leverage the diverse capabilities of its members to advance genomic resources for amphibians and bridge the implementation gap between biologists, bioinformaticians, and conservation practitioners. Here we evaluate the state of the field of amphibian genomics, highlight previous studies, present challenges to overcome, and call on the research and conservation communities to unite as part of the AGC to enable amphibian genomics research to "leap" to the next level.

两栖动物是四足动物中的一个多样化类群,其三个系统目和科之间的分化时间较长。通过基因组学的视角研究两栖动物生物学,可以加深我们对这一动物类别和其他陆生脊椎动物特征的了解。由于两栖动物面临的威胁越来越大,我们对两栖动物基因组资源的需求比以往任何时候都更加迫切。两栖动物是最濒危的分类群之一,由于栖息地丧失、土地使用模式改变、疾病、气候变化及其协同效应,约有 41% 的物种濒临灭绝。两栖动物基因组资源使人们能够更好地了解其本体多样性、组织再生、不同的生活史和繁殖模式、抗捕食者策略以及恢复力和适应性反应。它们还是研究广泛基因组特征(如进化基因组扩展和收缩)的重要模型,因为在所有动物分类群中,它们的基因组大小范围最广,并拥有多种遗传性别决定机制。尽管有这些特点,两栖类动物的基因组测序工作却明显落后于其他脊椎动物,主要原因是组装两栖类动物庞大、重复丰富的基因组所面临的挑战,以及相对缺乏社会支持。长读数测序技术的出现,再加上先进的分子和计算技术,改善了脚手架结构,减少了计算工作量,使解决其中一些难题成为可能。为了通过国际协调与合作促进和加快两栖动物基因组学研究的生产和使用,我们于2023年初发起了两栖动物基因组学联盟(AGC,https://mvs.unimelb.edu.au/amphibian-genomics-consortium )。这个新兴社区已经拥有来自 41 个国家的 282 多名成员。两栖动物基因组学联盟旨在利用其成员的不同能力来推动两栖动物基因组资源的发展,并缩小生物学家、生物信息学家和保护工作者之间的实施差距。在此,我们对两栖动物基因组学领域的现状进行了评估,重点介绍了以往的研究,提出了需要克服的挑战,并呼吁研究界和保护界作为 AGC 的一部分团结起来,使两栖动物基因组学研究 "飞跃 "到新的水平。
{"title":"The Amphibian Genomics Consortium: advancing genomic and genetic resources for amphibian research and conservation.","authors":"Tiffany A Kosch, María Torres-Sánchez, H Christoph Liedtke, Kyle Summers, Maximina H Yun, Andrew J Crawford, Simon T Maddock, Md Sabbir Ahammed, Victor L N Araújo, Lorenzo V Bertola, Gary M Bucciarelli, Albert Carné, Céline M Carneiro, Kin O Chan, Ying Chen, Angelica Crottini, Jessica M da Silva, Robert D Denton, Carolin Dittrich, Gonçalo Espregueira Themudo, Katherine A Farquharson, Natalie J Forsdick, Edward Gilbert, Jing Che, Barbara A Katzenback, Ramachandran Kotharambath, Nicholas A Levis, Roberto Márquez, Glib Mazepa, Kevin P Mulder, Hendrik Müller, Mary J O'Connell, Pablo Orozco-terWengel, Gemma Palomar, Alice Petzold, David W Pfennig, Karin S Pfennig, Michael S Reichert, Jacques Robert, Mark D Scherz, Karen Siu-Ting, Anthony A Snead, Matthias Stöck, Adam M M Stuckert, Jennifer L Stynoski, Rebecca D Tarvin, Katharina C Wollenberg Valero","doi":"10.1186/s12864-024-10899-7","DOIUrl":"10.1186/s12864-024-10899-7","url":null,"abstract":"<p><p>Amphibians represent a diverse group of tetrapods, marked by deep divergence times between their three systematic orders and families. Studying amphibian biology through the genomics lens increases our understanding of the features of this animal class and that of other terrestrial vertebrates. The need for amphibian genomic resources is more urgent than ever due to the increasing threats to this group. Amphibians are one of the most imperiled taxonomic groups, with approximately 41% of species threatened with extinction due to habitat loss, changes in land use patterns, disease, climate change, and their synergistic effects. Amphibian genomic resources have provided a better understanding of ontogenetic diversity, tissue regeneration, diverse life history and reproductive modes, anti-predator strategies, and resilience and adaptive responses. They also serve as essential models for studying broad genomic traits, such as evolutionary genome expansions and contractions, as they exhibit the widest range of genome sizes among all animal taxa and possess multiple mechanisms of genetic sex determination. Despite these features, genome sequencing of amphibians has significantly lagged behind that of other vertebrates, primarily due to the challenges of assembling their large, repeat-rich genomes and the relative lack of societal support. The emergence of long-read sequencing technologies, combined with advanced molecular and computational techniques that improve scaffolding and reduce computational workloads, is now making it possible to address some of these challenges. To promote and accelerate the production and use of amphibian genomics research through international coordination and collaboration, we launched the Amphibian Genomics Consortium (AGC, https://mvs.unimelb.edu.au/amphibian-genomics-consortium ) in early 2023. This burgeoning community already has more than 282 members from 41 countries. The AGC aims to leverage the diverse capabilities of its members to advance genomic resources for amphibians and bridge the implementation gap between biologists, bioinformaticians, and conservation practitioners. Here we evaluate the state of the field of amphibian genomics, highlight previous studies, present challenges to overcome, and call on the research and conservation communities to unite as part of the AGC to enable amphibian genomics research to \"leap\" to the next level.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic diversities and drug resistance in Mycobacterium bovis isolates from zoonotic tuberculosis using whole genome sequencing. 利用全基因组测序分析人畜共患病结核分枝杆菌分离物的遗传多样性和耐药性。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s12864-024-10909-8
Noha Salah Soliman, May Sherif Soliman, Sahar Mohammed Khairat, Maha Ali Gad, Sherine Shawky, Amani Ali Elkholy

Background: Zoonotic human tuberculosis (TB) caused by Mycobacterium bovis (M. bovis) is as vital as Mycobacterium tuberculosis, however with scarce available information. We aimed to use whole-genome sequencing (WGS) technology to take a deep insight into the circulating genotypes of human M. bovis and the genomic characteristics underlying virulence and drug resistance.

Methods: The study included smear positive Ziehl-Neelsen samples from patients with suspected tuberculosis. Samples were cultured on Lowenstein-Jensen media and suspected colonies of M. bovis were selected to undergo DNA extraction and WGS. Data was analysed using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC), and online bioinformatics tools. A phylogenetic tree was constructed for our sequenced strains, in addition to a set of 59 previously sequenced M. bovis genomes from different hosts and countries.

Results: Out of total 112 mycobacterial positive cultures, five M. bovis were isolated and underwent WGS. All sequenced strains belonged to Mycobacterium tuberculosis var bovis, spoligotype BOV_1; BOV_11. Resistance gene mutations were determined in 100% of strains to pyrazinamide (pncA and rpsA), isoniazid (KatG and ahpC), ethambutol (embB, embC, embR and ubiA), streptomycin (rpsl) and fluoroquinolones (gyrA and gyrB). Rifampin (rpoB and rpoC) and delamanid (fbiC) resistance genes were found in 80% of strains. The major represented virulence classes were the secretion system, cell surface components and regulation system. The phylogenetic analysis revealed close genetic relatedness of three sequenced M. bovis strains to previous reported cow strains from Egypt and human strains from France, as well as relatedness of one M. bovis strain to four human Algerian strains. One sequenced strain was related to one cow strain from Egypt and a human strain from South Africa.

Conclusions: All sequenced M. bovis isolates showed the same spoligotype, but diverse phylogeny. Resistance gene mutations were detected for anti-TB drugs including pyrazinamide, isoniazid, streptomycin, ethambutol, fluoroquinolones, cycloserine, rifampin and delamanid. The virulence profile comprised genes assigned mainly to secretion system, cell surface components and regulation system. Phylogenetic analysis revealed genetic relatedness between our isolates and previously sequenced bovine strains from Egypt as well as human strains from other nearby countries in the region.

背景:由牛分枝杆菌(M. bovis)引起的人畜共患人类结核病(TB)与结核分枝杆菌一样重要,但可用信息却很少。我们的目的是利用全基因组测序(WGS)技术深入了解人牛分枝杆菌的循环基因型以及毒力和耐药性的基因组特征:研究对象包括疑似肺结核患者的涂片阳性 Ziehl-Neelsen 样本。样本在 Lowenstein-Jensen 培养基上进行培养,选出疑似牛杆菌菌落进行 DNA 提取和 WGS 分析。使用细菌和病毒生物信息学资源中心(BV-BRC)和在线生物信息学工具对数据进行分析。除了一组先前测序的来自不同宿主和国家的 59 个牛分枝杆菌基因组外,还为我们测序的菌株构建了一棵系统发生树:结果:在总计 112 个分枝杆菌阳性培养物中,有 5 株牛分枝杆菌被分离出来并进行了 WGS 测序。所有测序菌株均属于结核分枝杆菌变种牛分枝杆菌,孢子型为 BOV_1; BOV_11。100%的菌株对吡嗪酰胺(gncA和rpsA)、异烟肼(KatG和ahpC)、乙胺丁醇(embB、embC、embR和ubiA)、链霉素(rpsl)和氟喹诺酮类(gyrA和gyrB)的耐药基因突变被确定。在 80% 的菌株中发现了利福平(rpoB 和 rpoC)和delamanid(fbiC)抗性基因。主要的毒力类型包括分泌系统、细胞表面成分和调节系统。系统发育分析表明,3株已测序的牛海绵状芽孢杆菌与之前报告的埃及奶牛菌株和法国人用菌株有密切的遗传亲缘关系,1株牛海绵状芽孢杆菌与4株阿尔及利亚人用菌株有亲缘关系。一个测序菌株与埃及的一个奶牛菌株和南非的一个人类菌株有亲缘关系:结论:所有已测序的牛海绵状芽孢杆菌分离株都显示出相同的spoligotype,但系统发育各不相同。检测到的抗结核药物耐药基因突变包括吡嗪酰胺、异烟肼、链霉素、乙胺丁醇、氟喹诺酮类、环丝氨酸、利福平和地拉那米德。毒力谱包括主要分配给分泌系统、细胞表面成分和调节系统的基因。系统发育分析表明,我们分离到的菌株与之前在埃及测序的牛菌株以及该地区其他邻近国家的人类菌株之间存在遗传亲缘关系。
{"title":"Genetic diversities and drug resistance in Mycobacterium bovis isolates from zoonotic tuberculosis using whole genome sequencing.","authors":"Noha Salah Soliman, May Sherif Soliman, Sahar Mohammed Khairat, Maha Ali Gad, Sherine Shawky, Amani Ali Elkholy","doi":"10.1186/s12864-024-10909-8","DOIUrl":"10.1186/s12864-024-10909-8","url":null,"abstract":"<p><strong>Background: </strong>Zoonotic human tuberculosis (TB) caused by Mycobacterium bovis (M. bovis) is as vital as Mycobacterium tuberculosis, however with scarce available information. We aimed to use whole-genome sequencing (WGS) technology to take a deep insight into the circulating genotypes of human M. bovis and the genomic characteristics underlying virulence and drug resistance.</p><p><strong>Methods: </strong>The study included smear positive Ziehl-Neelsen samples from patients with suspected tuberculosis. Samples were cultured on Lowenstein-Jensen media and suspected colonies of M. bovis were selected to undergo DNA extraction and WGS. Data was analysed using the Bacterial and Viral Bioinformatics Resource Center (BV-BRC), and online bioinformatics tools. A phylogenetic tree was constructed for our sequenced strains, in addition to a set of 59 previously sequenced M. bovis genomes from different hosts and countries.</p><p><strong>Results: </strong>Out of total 112 mycobacterial positive cultures, five M. bovis were isolated and underwent WGS. All sequenced strains belonged to Mycobacterium tuberculosis var bovis, spoligotype BOV_1; BOV_11. Resistance gene mutations were determined in 100% of strains to pyrazinamide (pncA and rpsA), isoniazid (KatG and ahpC), ethambutol (embB, embC, embR and ubiA), streptomycin (rpsl) and fluoroquinolones (gyrA and gyrB). Rifampin (rpoB and rpoC) and delamanid (fbiC) resistance genes were found in 80% of strains. The major represented virulence classes were the secretion system, cell surface components and regulation system. The phylogenetic analysis revealed close genetic relatedness of three sequenced M. bovis strains to previous reported cow strains from Egypt and human strains from France, as well as relatedness of one M. bovis strain to four human Algerian strains. One sequenced strain was related to one cow strain from Egypt and a human strain from South Africa.</p><p><strong>Conclusions: </strong>All sequenced M. bovis isolates showed the same spoligotype, but diverse phylogeny. Resistance gene mutations were detected for anti-TB drugs including pyrazinamide, isoniazid, streptomycin, ethambutol, fluoroquinolones, cycloserine, rifampin and delamanid. The virulence profile comprised genes assigned mainly to secretion system, cell surface components and regulation system. Phylogenetic analysis revealed genetic relatedness between our isolates and previously sequenced bovine strains from Egypt as well as human strains from other nearby countries in the region.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First report and genomic characterization of Escherichia coli O111:H12 serotype from raw mussels in Türkiye. 首次报告图尔基耶生贻贝中的大肠杆菌 O111:H12 血清型及其基因组特征。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s12864-024-10945-4
Artun Yibar, Nihed Ajmi, Muhammed Duman

Background: This study aimed to assess the prevalence and genomic characteristics of Shiga-toxigenic (STEC) and Enteroaggregative E. coli (EAEC) strains in raw mussels and ready-to-eat (RTE)-stuffed mussels, focusing on potential public health implications for identifying virulence and antimicrobial resistance genes.

Results: The genome sequence analysis identified the E. coli strain named 23EM as serotype O111:H12, with adhesion (fimH-54) and fumarate hydratase (fumC-11) genes. The draft genome (4.9 Mb, 50.6% GC content, 111 contigs, 4,688 genes) is available in NCBI GenBank (accession JAWXVJ000000000). The strain, classified as ST292 and CC ST10, showed high similarity to nonpathogenic E. coli MG1655 but was distinct from pathogenic strains such as EAEC and ExPEC. In silico serotyping revealed the presence of O111-antigen flippase (wzx) and H12-antigen flagellin (fliC) genes. The strain harbors an IncFII (pCoo) plasmid with 96.95% identity. PathogenFinder predicted a 92% probability of being a human pathogen, supported by 720 pathogenic protein families. CRISPR analysis identified one high-evidence sequence with nine spacers and six low-evidence sequences. Phylogenetic analysis using RAxML positioned 23EM close to nonpathogenic E. coli but distant from other pathogenic strains. Antimicrobial resistance genes across multiple classes, including macrolides, fluoroquinolones, and aminoglycosides, were identified. The strain also contains several virulence factors, such as adhesins (e.g., ECP, ELF, TIF, type IV pili), and autotransporter genes (espP, pic), highlighting its significant pathogenic potential and public health risk.

Conclusions: This study highlights the ability of the detection of E. coli strains harboring virulence and antimicrobial resistance genes in mussels, thus emphasizing the importance of ongoing surveillance and careful consideration of the potential risks associated with the consumption of these shellfish.

背景:本研究旨在评估生贻贝和即食(RTE)填料贻贝中志贺致毒大肠杆菌(STEC)和肠道聚集性大肠杆菌(EAEC)菌株的流行率和基因组特征,重点是确定毒力基因和抗菌药耐药性基因对公共卫生的潜在影响:基因组序列分析确定了名为 23EM 的大肠杆菌菌株为血清型 O111:H12,具有粘附(fimH-54)和富马酸水合酶(fumC-11)基因。基因组草案(4.9 Mb,50.6% GC 含量,111 个等位基因,4,688 个基因)可在 NCBI GenBank(登录号 JAWXVJ000000000)上查阅。该菌株被归类为 ST292 和 CC ST10,与非致病性大肠杆菌 MG1655 具有高度相似性,但与 EAEC 和 ExPEC 等致病性菌株不同。默克血清分型显示,该菌株存在 O111 抗原鞭毛蛋白酶(wzx)和 H12 抗原鞭毛蛋白(fliC)基因。该菌株携带的 IncFII(pCoo)质粒具有 96.95% 的同一性。根据 720 个致病蛋白家族的支持,PathogenFinder 预测其成为人类病原体的可能性为 92%。CRISPR 分析确定了一个带有九个间隔的高证据序列和六个低证据序列。使用 RAxML 进行的系统发育分析将 23EM 定位为接近非致病性大肠杆菌,但远离其他致病性菌株。鉴定出了多类抗菌药耐药性基因,包括大环内酯类、氟喹诺酮类和氨基糖苷类。该菌株还含有多种毒力因子,如粘附素(如 ECP、ELF、TIF、IV 型纤毛虫)和自转运基因(espP、pic),突显了其巨大的致病潜力和公共卫生风险:本研究强调了在贻贝中检测携带毒力基因和抗菌药耐药性基因的大肠杆菌菌株的能力,从而强调了持续监测和仔细考虑与食用这些贝类相关的潜在风险的重要性。
{"title":"First report and genomic characterization of Escherichia coli O111:H12 serotype from raw mussels in Türkiye.","authors":"Artun Yibar, Nihed Ajmi, Muhammed Duman","doi":"10.1186/s12864-024-10945-4","DOIUrl":"10.1186/s12864-024-10945-4","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to assess the prevalence and genomic characteristics of Shiga-toxigenic (STEC) and Enteroaggregative E. coli (EAEC) strains in raw mussels and ready-to-eat (RTE)-stuffed mussels, focusing on potential public health implications for identifying virulence and antimicrobial resistance genes.</p><p><strong>Results: </strong>The genome sequence analysis identified the E. coli strain named 23EM as serotype O111:H12, with adhesion (fimH-54) and fumarate hydratase (fumC-11) genes. The draft genome (4.9 Mb, 50.6% GC content, 111 contigs, 4,688 genes) is available in NCBI GenBank (accession JAWXVJ000000000). The strain, classified as ST292 and CC ST10, showed high similarity to nonpathogenic E. coli MG1655 but was distinct from pathogenic strains such as EAEC and ExPEC. In silico serotyping revealed the presence of O111-antigen flippase (wzx) and H12-antigen flagellin (fliC) genes. The strain harbors an IncFII (pCoo) plasmid with 96.95% identity. PathogenFinder predicted a 92% probability of being a human pathogen, supported by 720 pathogenic protein families. CRISPR analysis identified one high-evidence sequence with nine spacers and six low-evidence sequences. Phylogenetic analysis using RAxML positioned 23EM close to nonpathogenic E. coli but distant from other pathogenic strains. Antimicrobial resistance genes across multiple classes, including macrolides, fluoroquinolones, and aminoglycosides, were identified. The strain also contains several virulence factors, such as adhesins (e.g., ECP, ELF, TIF, type IV pili), and autotransporter genes (espP, pic), highlighting its significant pathogenic potential and public health risk.</p><p><strong>Conclusions: </strong>This study highlights the ability of the detection of E. coli strains harboring virulence and antimicrobial resistance genes in mussels, thus emphasizing the importance of ongoing surveillance and careful consideration of the potential risks associated with the consumption of these shellfish.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11531133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SWATH-MS based proteomics reveals the role of photosynthesis related proteins and secondary metabolic pathways in the colored leaves of sweet olive (Osmanthus fragrans). 基于 SWATH-MS 的蛋白质组学揭示了甜橄榄(Osmanthus fragrans)彩色叶片中光合作用相关蛋白质和次级代谢途径的作用。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 DOI: 10.1186/s12864-024-10867-1
Cheng Zhang, Kailu Zhang, Min Zhang, Daowu Zhang, Qi Ye, Xianrong Wang, Takashi Akagi, Yifan Duan

Colored leaves, a notable horticultural trait, have high research and ornamental value. The evergreen sweet olive (Osmanthus fragrans), one of the top ten traditional flowers in China, has been cultivated for more than two thousand years. However, in recent years, an increasing number of O. fragrans cultivars with colored leaves have been cultivated for their ornamental value. To study the molecular mechanism underlying the observed changes in leaf color, we selected O. fragrans 'Yinbi Shuanghui' (Y), which has yellow-white leaves, and O. fragrans 'Sijigui' (S), which has green leaves, as materials. Pigment content measurement showed that the chlorophyll, carotenoid and anthocyanin contents in Y were lower than in S. According to the SWATH-MS sequencing results, a total of 3,959 proteins were quantitatively identified, 1,300 of which were differentially expressed proteins (DEPs), including 782 up-regulated and 518 down-regulated proteins in Y compared to S. Functional enrichment analysis of DEPs revealed that down-regulated expression of photosynthesis related proteins may lead to the inhibition of chlorophyll synthesis in Y, this may be the main cause of leaf color change. Moreover, a protein interaction prediction model also showed that proteins such as PetC, PsbO, PsbP, and PsbQ were key proteins in the interaction network, and the up-regulated proteins participating in the anthocyanin and carotenoid pathways may be related to the formation of yellow-white leaves. Taken together, our findings represent the first SWATH-MS-based proteomic report on colored leaf O. fragrans and reveal that chlorophyll synthesis and secondary metabolism pathways contribute to the changes in leaf color.

彩叶是一种显著的园艺特征,具有很高的研究和观赏价值。常绿橄榄(Osmanthus fragrans)是中国十大传统名花之一,已有两千多年的栽培历史。然而,近年来,越来越多具有彩色叶片的橄榄栽培品种因其观赏价值而被栽培。为了研究观察到的叶片颜色变化的分子机制,我们选择了叶片为黄白色的香紫苏'银碧双辉'(Y)和叶片为绿色的香紫苏'四季桂'(S)作为材料。根据 SWATH-MS 测序结果,共定量鉴定出 3959 个蛋白质,其中 1300 个为差异表达蛋白质(DEPs),包括 782 个上调蛋白质和 518 个下调蛋白质。DEPs 的功能富集分析表明,光合作用相关蛋白的表达下调可能导致叶绿素合成受到抑制,这可能是叶色变化的主要原因。此外,蛋白质相互作用预测模型也表明,PetC、PsbO、PsbP 和 PsbQ 等蛋白质是相互作用网络中的关键蛋白质,而参与花青素和类胡萝卜素途径的蛋白质上调可能与黄白色叶片的形成有关。综上所述,我们的研究结果代表了首个基于 SWATH-MS 的彩叶欧鼠李蛋白质组学报告,揭示了叶绿素合成和次生代谢途径有助于叶片颜色的变化。
{"title":"SWATH-MS based proteomics reveals the role of photosynthesis related proteins and secondary metabolic pathways in the colored leaves of sweet olive (Osmanthus fragrans).","authors":"Cheng Zhang, Kailu Zhang, Min Zhang, Daowu Zhang, Qi Ye, Xianrong Wang, Takashi Akagi, Yifan Duan","doi":"10.1186/s12864-024-10867-1","DOIUrl":"10.1186/s12864-024-10867-1","url":null,"abstract":"<p><p>Colored leaves, a notable horticultural trait, have high research and ornamental value. The evergreen sweet olive (Osmanthus fragrans), one of the top ten traditional flowers in China, has been cultivated for more than two thousand years. However, in recent years, an increasing number of O. fragrans cultivars with colored leaves have been cultivated for their ornamental value. To study the molecular mechanism underlying the observed changes in leaf color, we selected O. fragrans 'Yinbi Shuanghui' (Y), which has yellow-white leaves, and O. fragrans 'Sijigui' (S), which has green leaves, as materials. Pigment content measurement showed that the chlorophyll, carotenoid and anthocyanin contents in Y were lower than in S. According to the SWATH-MS sequencing results, a total of 3,959 proteins were quantitatively identified, 1,300 of which were differentially expressed proteins (DEPs), including 782 up-regulated and 518 down-regulated proteins in Y compared to S. Functional enrichment analysis of DEPs revealed that down-regulated expression of photosynthesis related proteins may lead to the inhibition of chlorophyll synthesis in Y, this may be the main cause of leaf color change. Moreover, a protein interaction prediction model also showed that proteins such as PetC, PsbO, PsbP, and PsbQ were key proteins in the interaction network, and the up-regulated proteins participating in the anthocyanin and carotenoid pathways may be related to the formation of yellow-white leaves. Taken together, our findings represent the first SWATH-MS-based proteomic report on colored leaf O. fragrans and reveal that chlorophyll synthesis and secondary metabolism pathways contribute to the changes in leaf color.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A stepwise guide for pangenome development in crop plants: an alfalfa (Medicago sativa) case study. 作物植物泛基因组开发的逐步指南:紫花苜蓿(Medicago sativa)案例研究。
IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-10-31 DOI: 10.1186/s12864-024-10931-w
Harpreet Kaur, Laura M Shannon, Deborah A Samac

Background: The concept of pangenomics and the importance of structural variants is gaining recognition within the plant genomics community. Due to advancements in sequencing and computational technology, it has become feasible to sequence the entire genome of numerous individuals of a single species at a reasonable cost. Pangenomes have been constructed for many major diploid crops, including rice, maize, soybean, sorghum, pearl millet, peas, sunflower, grapes, and mustards. However, pangenomes for polyploid species are relatively scarce and are available in only few crops including wheat, cotton, rapeseed, and potatoes.

Main body: In this review, we explore the various methods used in crop pangenome development, discussing the challenges and implications of these techniques based on insights from published pangenome studies. We offer a systematic guide and discuss the tools available for constructing a pangenome and conducting downstream analyses. Alfalfa, a highly heterozygous, cross pollinated and autotetraploid forage crop species, is used as an example to discuss the concerns and challenges offered by polyploid crop species. We conducted a comparative analysis using linear and graph-based methods by constructing an alfalfa graph pangenome using three publicly available genome assemblies. To illustrate the intricacies captured by pangenome graphs for a complex crop genome, we used five different gene sequences and aligned them against the three graph-based pangenomes. The comparison of the three graph pangenome methods reveals notable variations in the genomic variation captured by each pipeline.

Conclusion: Pangenome resources are proving invaluable by offering insights into core and dispensable genes, novel gene discovery, and genome-wide patterns of variation. Developing user-friendly online portals for linear pangenome visualization has made these resources accessible to the broader scientific and breeding community. However, challenges remain with graph-based pangenomes including compatibility with other tools, extraction of sequence for regions of interest, and visualization of genetic variation captured in pangenome graphs. These issues necessitate further refinement of tools and pipelines to effectively address the complexities of polyploid, highly heterozygous, and cross-pollinated species.

背景:泛基因组学的概念和结构变异的重要性正在得到植物基因组学界的认可。由于测序和计算技术的进步,以合理的成本对单一物种的众多个体进行全基因组测序已变得可行。目前已经构建了许多主要二倍体作物的庞基因组,包括水稻、玉米、大豆、高粱、珍珠粟、豌豆、向日葵、葡萄和芥菜。然而,多倍体物种的泛基因组相对较少,只有小麦、棉花、油菜籽和马铃薯等少数作物有泛基因组:在这篇综述中,我们探讨了作物泛基因组开发中使用的各种方法,并根据已发表的泛基因组研究结果,讨论了这些技术所面临的挑战和意义。我们提供了一个系统指南,并讨论了构建庞基因组和进行下游分析的可用工具。紫花苜蓿是一种高度杂合、异花授粉和自交四倍体的饲料作物物种,我们以紫花苜蓿为例,讨论了多倍体作物物种带来的问题和挑战。我们使用线性方法和基于图谱的方法进行了比较分析,利用三个公开的基因组汇编构建了紫花苜蓿图谱泛基因组。为了说明庞基因组图谱捕捉到的复杂作物基因组的错综复杂性,我们使用了五个不同的基因序列,并将它们与三个基于图谱的庞基因组进行了比对。对三种图谱庞基因组方法进行比较后发现,每种方法捕获的基因组变异都存在明显差异:通过深入了解核心基因和可有可无的基因、新基因的发现以及全基因组的变异模式,庞基因组资源被证明是非常宝贵的。为线性庞基因组可视化开发用户友好型在线门户网站,使更广泛的科学界和育种界可以访问这些资源。然而,基于图谱的庞基因组仍然面临挑战,包括与其他工具的兼容性、感兴趣区域的序列提取以及庞基因组图谱中捕获的遗传变异的可视化。这些问题需要进一步完善工具和管道,以有效解决多倍体、高度杂合和异花授粉物种的复杂性。
{"title":"A stepwise guide for pangenome development in crop plants: an alfalfa (Medicago sativa) case study.","authors":"Harpreet Kaur, Laura M Shannon, Deborah A Samac","doi":"10.1186/s12864-024-10931-w","DOIUrl":"10.1186/s12864-024-10931-w","url":null,"abstract":"<p><strong>Background: </strong>The concept of pangenomics and the importance of structural variants is gaining recognition within the plant genomics community. Due to advancements in sequencing and computational technology, it has become feasible to sequence the entire genome of numerous individuals of a single species at a reasonable cost. Pangenomes have been constructed for many major diploid crops, including rice, maize, soybean, sorghum, pearl millet, peas, sunflower, grapes, and mustards. However, pangenomes for polyploid species are relatively scarce and are available in only few crops including wheat, cotton, rapeseed, and potatoes.</p><p><strong>Main body: </strong>In this review, we explore the various methods used in crop pangenome development, discussing the challenges and implications of these techniques based on insights from published pangenome studies. We offer a systematic guide and discuss the tools available for constructing a pangenome and conducting downstream analyses. Alfalfa, a highly heterozygous, cross pollinated and autotetraploid forage crop species, is used as an example to discuss the concerns and challenges offered by polyploid crop species. We conducted a comparative analysis using linear and graph-based methods by constructing an alfalfa graph pangenome using three publicly available genome assemblies. To illustrate the intricacies captured by pangenome graphs for a complex crop genome, we used five different gene sequences and aligned them against the three graph-based pangenomes. The comparison of the three graph pangenome methods reveals notable variations in the genomic variation captured by each pipeline.</p><p><strong>Conclusion: </strong>Pangenome resources are proving invaluable by offering insights into core and dispensable genes, novel gene discovery, and genome-wide patterns of variation. Developing user-friendly online portals for linear pangenome visualization has made these resources accessible to the broader scientific and breeding community. However, challenges remain with graph-based pangenomes including compatibility with other tools, extraction of sequence for regions of interest, and visualization of genetic variation captured in pangenome graphs. These issues necessitate further refinement of tools and pipelines to effectively address the complexities of polyploid, highly heterozygous, and cross-pollinated species.</p>","PeriodicalId":9030,"journal":{"name":"BMC Genomics","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11526573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
BMC Genomics
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1