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Methionine enkephalin(MENK) upregulated memory T cells in anti-influenza response. 甲硫氨酸脑啡肽(MENK)在抗流感反应中上调记忆T细胞。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-12 DOI: 10.1186/s12865-023-00573-0
Jing Tian, Wenrui Fu, Zifeng Xie, Xiaonan Wang, Miao Miao, Fengping Shan, Xiaodong Yu

Novel prophylactic drugs and vaccination strategies for protection against influenza virus should induce specific effector T-cell immune responses in pulmonary airways and peripheral lymphoid organs. Designing approaches that promote T-cell-mediated responses and memory T-cell differentiation would strengthen host resistance to respiratory infectious diseases. The results of this study showed that pulmonary delivery of MENK via intranasal administration reduced viral titres, upregulated opioid receptor MOR and DOR, increased the proportions of T-cell subsets including CD8+ T cells, CD8+ TEM cells, NP/PA-effector CD8+ TEM cells in bronchoalveolar lavage fluid and lungs, and CD4+/CD8+ TCM cells in lymph nodes to protect mice against influenza viral challenge. Furthermore, we demonstrated that, on the 10th day of infection, the proportions of CD4+ TM and CD8+ TM cells were significantly increased, which meant that a stable TCM and TEM lineage was established in the early stage of influenza infection. Collectively, our data suggested that MENK administered intranasally, similar to the route of natural infection by influenza A virus, could exert antiviral activity through upregulating T-cell-mediated adaptive immune responses against influenza virus.

预防流感病毒的新型预防药物和疫苗接种策略应在肺气道和外周淋巴器官中诱导特异性效应T细胞免疫反应。设计促进T细胞介导的反应和记忆T细胞分化的方法将增强宿主对呼吸道传染病的抵抗力。本研究的结果表明,通过鼻内给药的MENK肺部给药降低了病毒滴度,上调了阿片受体MOR和DOR,增加了支气管肺泡灌洗液和肺部T细胞亚群的比例,包括CD8+T细胞、CD8+TEM细胞、NP/PA效应CD8+TEM细胞,以及淋巴结中的CD4+/CD8+TCM细胞,以保护小鼠免受流感病毒攻击。此外,我们证明,在感染的第10天,CD4+TM和CD8+TM细胞的比例显著增加,这意味着在流感感染的早期阶段建立了稳定的TCM和TEM谱系。总之,我们的数据表明,类似于甲型流感病毒的自然感染途径,鼻内给药MENK可以通过上调T细胞介导的针对流感病毒的适应性免疫反应来发挥抗病毒活性。
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引用次数: 0
Transcriptomic analysis identifies CYP27A1 as a diagnostic marker for the prognosis and immunity in lung adenocarcinoma. 转录组学分析确定CYP27A1是肺腺癌预后和免疫的诊断标志物。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-10 DOI: 10.1186/s12865-023-00572-1
Yi Yin, Muqun He, Yunjian Huang, Xianhe Xie

Background: The association between lipid metabolism disorder and carcinogenesis is well-established, but there is limited research on the connection between lipid metabolism-related genes (LRGs) and lung adenocarcinoma (LUAD). The objective of our research was to identify LRGs as the potential biomarkers for prognosis and assess their impact on immune cell infiltration in LUAD.

Methods: We identified novel prognostic LRGs for LUAD patients via the bioinformatics analysis. CYP27A1 expression level was systematically evaluated via various databases, such as TCGA, UALCAN, and TIMER. Subsequently, LinkedOmics was utilized to perform the CYP27A1 co-expression network and GSEA. ssGSEA was conducted to assess the association between infiltration of immune cells and CYP27A1 expression. CYP27A1's expression level was validated by qRT-PCR analysis.

Results: CYP27A1 expression was decreased in LUAD. Reduced CYP27A1 expression was linked to unfavorable prognosis in LUAD. Univariate and multivariate analyses indicated that CYP27A1 was an independent prognostic biomarker for LUAD patients. GSEA results revealed a positive correlation between CYP27A1 expression and immune-related pathways. Furthermore, CYP27A1 expression was positively correlated with the infiltration levels of most immune cells.

Conclusion: CYP27A1 is a potential biomarker for LUAD patients, and our findings provided a novel perspective to develop the prognostic marker for LUAD patients.

背景:脂质代谢紊乱与致癌作用之间的联系已经得到证实,但对脂质代谢相关基因(LRGs)与肺腺癌(LUAD)之间的联系的研究有限。我们的研究目的是确定LRG作为潜在的预后生物标志物,并评估其对LUAD免疫细胞浸润的影响。方法:通过生物信息学分析,我们为LUAD患者确定了新的预后LRG。CYP27A1的表达水平通过各种数据库进行系统评估,如TCGA、UALCAN和TIMER。随后,利用LinkedOmics进行CYP27A1共表达网络和GSEA。进行ssGSEA以评估免疫细胞浸润与CYP27A1表达之间的相关性。通过qRT-PCR分析验证CYP27A1的表达水平。结果:CYP27A1在LUAD中的表达降低。CYP27A1表达降低与LUAD的不良预后有关。单变量和多变量分析表明CYP27A1是LUAD患者的独立预后生物标志物。GSEA结果显示CYP27A1的表达与免疫相关途径呈正相关。此外,CYP27A1的表达与大多数免疫细胞的浸润水平呈正相关。结论:CYP27A1是LUAD患者潜在的生物标志物,我们的研究结果为开发LUAD患者的预后标志物提供了新的视角。
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引用次数: 1
Bcl-3 regulates T cell function through energy metabolism. Bcl-3通过能量代谢调节T细胞功能。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-04 DOI: 10.1186/s12865-023-00570-3
Hui Liu, Lin Zeng, Mengmeng Pan, Liwenhui Huang, Hanying Li, Mengxia Liu, Xinqing Niu, Chenguang Zhang, Hui Wang

Background: Bcl-3 is a member of the IκB protein family and an essential modulator of NF-κB activity. It is well established that Bcl-3 is critical for the normal development, survival and differentiation of adaptive immune cells, especially T cells. However, the regulation of immune cell function by Bcl-3 through metabolic pathways has rarely been studied.

Results: In this study, we explored the role of Bcl-3 in the metabolism and function of T cells via the mTOR pathway. We verified that the proliferation of Bcl-3-deficient Jurkat T cells was inhibited, but their activation was promoted, and Bcl-3 depletion regulated cellular energy metabolism by reducing intracellular ATP and ROS production levels and mitochondrial membrane potential. Bcl-3 also regulates cellular energy metabolism in naive CD4+ T cells. In addition, the knockout of Bcl-3 altered the expression of mTOR, Akt, and Raptor, which are metabolism-related genes, in Jurkat cells.

Conclusions: This finding indicates that Bcl-3 may mediate the energy metabolism of T cells through the mTOR pathway, thereby affecting their function. Overall, we provide novel insights into the regulatory role of Bcl-3 in T-cell energy metabolism for the prevention and treatment of immune diseases.

背景:Bcl-3是IκB蛋白家族的成员,也是NF-κB活性的重要调节剂。Bcl-3对适应性免疫细胞,特别是T细胞的正常发育、存活和分化至关重要。然而,Bcl-3通过代谢途径调节免疫细胞功能的研究很少。结果:在本研究中,我们通过mTOR途径探讨了Bcl-3在T细胞代谢和功能中的作用。我们证实Bcl-3缺陷的Jurkat T细胞的增殖受到抑制,但它们的激活被促进,Bcl-3缺失通过降低细胞内ATP和ROS的产生水平以及线粒体膜电位来调节细胞能量代谢。Bcl-3还调节幼稚CD4+T细胞的细胞能量代谢。此外,Bcl-3的敲除改变了Jurkat细胞中代谢相关基因mTOR、Akt和Raptor的表达。结论:Bcl-3可能通过mTOR途径介导T细胞的能量代谢,从而影响其功能。总之,我们对Bcl-3在T细胞能量代谢中的调节作用提供了新的见解,用于预防和治疗免疫疾病。
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引用次数: 0
The digestive system and autoimmunity. 消化系统和自身免疫。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-10-04 DOI: 10.1186/s12865-023-00561-4
Lina Sun, Baojun Zhang

Digestive autoimmune conditions are a growing challenge to global health. Risk factors associated with autoimmune digestive diseases are complex, including genetic variation, immunological dysfunction, and various environmental factors. To improve our understanding of the mechanisms behind digestive autoimmune conditions, including factors causing gastrointestinal manifestations and pathogenesis, BMC Immunology has launched a new Collection "The digestive system and autoimmunity".

消化系统自身免疫性疾病对全球健康构成越来越大的挑战。与自身免疫性消化系统疾病相关的危险因素是复杂的,包括遗传变异、免疫功能障碍和各种环境因素。为了更好地了解消化系统自身免疫性疾病背后的机制,包括引起胃肠道表现和发病机制的因素,BMC免疫学推出了一个新的集合“消化系统和自身免疫”。
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引用次数: 0
Function and autophagy of monocyte-derived dendritic cells is affected by hepatitis B virus infection. 单核细胞来源的树突状细胞的功能和自噬受到乙型肝炎病毒感染的影响。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-26 DOI: 10.1186/s12865-023-00571-2
Hua Xu, Juan Kang, Shan Zhong, Min Chen, Peng Hu, Hong Ren, Zhi Zhou, Yu Lei

Background: The role of dendritic cells and the autophagy state of dendritic cells in the immune response of hepatitis B virus (HBV) infection was still controversial. In this study, we carefully examined the phenotype, function and autophagy pathway of dendritic cells in HBV infection.

Methods: Monocyte-derived dendritic cells from healthy blood donors and patients with chronic HBV infection were stimulated by lipopolysaccharide, supernatant of HepG2.2.15 cells or supernatant of HepG2 cells respectively. Phenotype of dendritic cells was examined by flow cytometry and cytokines secretion was detected by enzyme-linked immunosorbent assay. Autophagy related proteins were detected by western blot and immunofluorescence analysis.

Results: Our results showed that the expression of both major histocompatibility complex II molecules and co-stimulated molecules including cluster of differentiation antigen 80, cluster of differentiation antigen 86 in the monocyte-derived dendritic cells from patients with chronic HBV infection was significantly higher than that from healthy donors when cultured with supernatant of HepG2.2.15 cells. The amount of cytokines, including tumour necrosis factor-α, interleukin-10 and interleukin-12, secreted by monocyte-derived dendritic cells from patients with chronic HBV infection was also significantly higher than that from healthy donors when stimulate by HBV. Interestingly, the expression level of autophagy-related proteins including autophagy-related protein5 and associated protein 1 light chain in dendritic cells from patients with chronic HBV infection was significantly increased when compared with that from healthy donors when re-exposed to HBV.

Conclusions: Our results indicated that dendritic cells from patients with chronic HBV infection could intensively present antigen and express co-stimulatory molecules. The increased activation of dendritic cells might be related to the enhanced autophagy of dendritic cells in HBV infection.

背景:树突状细胞和树突状细胞的自噬状态在乙型肝炎病毒(HBV)感染的免疫反应中的作用仍然存在争议。在本研究中,我们仔细检查了HBV感染中树突状细胞的表型、功能和自噬途径。方法:分别用脂多糖、HepG2.2.15细胞上清液和HepG2细胞上清液刺激健康献血者和慢性HBV感染者的单核细胞来源的树突状细胞。流式细胞仪检测树突状细胞表型,酶联免疫吸附法检测细胞因子分泌。通过蛋白质印迹和免疫荧光分析检测自噬相关蛋白。结果:主要组织相容性复合体II分子和共刺激分子(包括分化抗原簇80,当与HepG2.2.15细胞的上清液培养时,来自慢性HBV感染患者的单核细胞衍生的树突状细胞中的分化抗原簇86显著高于来自健康供体的分化抗原。当HBV刺激时,慢性HBV感染患者的单核细胞衍生的树突状细胞分泌的细胞因子,包括肿瘤坏死因子-α、白细胞介素-10和白细胞介质-12的量也显著高于健康供体。有趣的是,与健康供体相比,慢性HBV感染患者树突状细胞中自噬相关蛋白(包括自噬相关蛋白质5和相关蛋白质1轻链)的表达水平显著升高呈递抗原并表达共刺激分子。树突状细胞活化的增加可能与HBV感染中树突状细胞自噬的增强有关。
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引用次数: 0
The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through membrane immunoassay device. 将新开发的单克隆抗体应用于自动化学发光流通膜免疫测定装置的高度灵敏和定量的严重急性呼吸系统综合征冠状病毒2型快速抗原检测的开发。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-26 DOI: 10.1186/s12865-023-00567-y
Kengo Nishimura, Hiroaki Kitazawa, Takashi Kawahata, Kosuke Yuhara, Takahiro Masuya, Toshihiro Kuroita, Kentarou Waki, Seiichi Koike, Masaharu Isobe, Nobuyuki Kurosawa

Background: Rapid and accurate diagnosis of individuals with SARS-CoV-2 infection is an effective way to prevent and control the spread of COVID-19. Although the detection of SARS-CoV-2 viral RNA by RT-qPCR is the gold standard for COVID-19 testing, the use of antigen-detecting rapid diagnostic tests (Ag-RDTs) is emerging as a complementary surveillance tool as Omicron case numbers skyrocket worldwide. However, the results from Ag-RDTs are less accurate in individuals with low viral loads.

Results: To develop a highly sensitive and accurate Ag-RDT, 90 monoclonal antibodies were raised from guinea pigs immunized with SARS CoV-2 nucleocapsid protein (CoV-2-NP). By applying a capture antibody recognizing the structural epitope of the N-terminal domain of CoV-2-NP and a detection antibody recognizing the C-terminal tail of CoV-2-NP to an automated chemiluminescence flow-through membrane immunoassay device, we developed a novel Ag-RDT, CoV-2-POCube. The CoV-2-POCube exclusively recognizes CoV-2-NP variants but not the nucleocapsid proteins of other human coronaviruses. The CoV-2-POCube achieved a limit of detection sensitivity of 0.20 ~ 0.66 pg/mL of CoV-2-NPs, demonstrating more than 100 times greater sensitivity than commercially available SARS-CoV-2 Ag-RDTs.

Conclusions: CoV-2-POCube has high analytical sensitivity and can detect SARS-CoV-2 variants in 15 min without observing the high-dose hook effect, thus meeting the need for early SARS-CoV-2 diagnosis with lower viral load. CoV-2-POCube is a promising alternative to currently available diagnostic devices for faster clinical decision making in individuals with suspected COVID-19 in resource-limited settings.

背景:快速准确地诊断SARS-CoV-2感染者是预防和控制新冠肺炎传播的有效途径。尽管RT-qPCR检测SARS-CoV-2病毒RNA是新冠肺炎检测的金标准,但随着全球奥密克戎病例数飙升,抗原检测快速诊断检测(Ag-RDT)的使用正在成为一种补充监测工具。然而,Ag-RDT的结果在病毒载量低的个体中不太准确。结果:从SARS冠状病毒2型核衣壳蛋白(CoV-2-NP)免疫的豚鼠身上制备了90株单克隆抗体,建立了一种高灵敏、准确的Ag-RDT。通过将识别CoV-2-NP N端结构域结构表位的捕获抗体和识别CoV-2-NP C端尾部的检测抗体应用于自动化学发光流通膜免疫测定装置,我们开发了一种新型的Ag-RDT,CoV-2-POCube。CoV-2-POCube只识别CoV-2-NP变体,但不识别其他人类冠状病毒的核衣壳蛋白。CoV-2-POCube实现了0.20的检测灵敏度极限 ~ 0.66 pg/mL的CoV-2-NP,显示出比市售的严重急性呼吸系统综合征冠状病毒2型Ag-RDT高100多倍的灵敏度。CoV-2-POCube是目前可用诊断设备的一种很有前途的替代品,可以在资源有限的环境中为疑似新冠肺炎患者更快地做出临床决策。
{"title":"The development of a highly sensitive and quantitative SARS-CoV-2 rapid antigen test applying newly developed monoclonal antibodies to an automated chemiluminescent flow-through membrane immunoassay device.","authors":"Kengo Nishimura, Hiroaki Kitazawa, Takashi Kawahata, Kosuke Yuhara, Takahiro Masuya, Toshihiro Kuroita, Kentarou Waki, Seiichi Koike, Masaharu Isobe, Nobuyuki Kurosawa","doi":"10.1186/s12865-023-00567-y","DOIUrl":"10.1186/s12865-023-00567-y","url":null,"abstract":"<p><strong>Background: </strong>Rapid and accurate diagnosis of individuals with SARS-CoV-2 infection is an effective way to prevent and control the spread of COVID-19. Although the detection of SARS-CoV-2 viral RNA by RT-qPCR is the gold standard for COVID-19 testing, the use of antigen-detecting rapid diagnostic tests (Ag-RDTs) is emerging as a complementary surveillance tool as Omicron case numbers skyrocket worldwide. However, the results from Ag-RDTs are less accurate in individuals with low viral loads.</p><p><strong>Results: </strong>To develop a highly sensitive and accurate Ag-RDT, 90 monoclonal antibodies were raised from guinea pigs immunized with SARS CoV-2 nucleocapsid protein (CoV-2-NP). By applying a capture antibody recognizing the structural epitope of the N-terminal domain of CoV-2-NP and a detection antibody recognizing the C-terminal tail of CoV-2-NP to an automated chemiluminescence flow-through membrane immunoassay device, we developed a novel Ag-RDT, CoV-2-POCube. The CoV-2-POCube exclusively recognizes CoV-2-NP variants but not the nucleocapsid proteins of other human coronaviruses. The CoV-2-POCube achieved a limit of detection sensitivity of 0.20 ~ 0.66 pg/mL of CoV-2-NPs, demonstrating more than 100 times greater sensitivity than commercially available SARS-CoV-2 Ag-RDTs.</p><p><strong>Conclusions: </strong>CoV-2-POCube has high analytical sensitivity and can detect SARS-CoV-2 variants in 15 min without observing the high-dose hook effect, thus meeting the need for early SARS-CoV-2 diagnosis with lower viral load. CoV-2-POCube is a promising alternative to currently available diagnostic devices for faster clinical decision making in individuals with suspected COVID-19 in resource-limited settings.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10523765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41103995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immune cell profiles of idiopathic inflammatory myopathy patients expressed anti-aminoacyl tRNA synthetase or anti-melanoma differentiation-associated gene 5 autoantibodies. 特发性炎性肌病患者的免疫细胞谱表达抗氨酰基tRNA合成酶或抗黑色素瘤分化相关基因5自身抗体。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-26 DOI: 10.1186/s12865-023-00569-w
Joung-Liang Lan, Shih-Hsin Chang, Gregory J Tsay, Der-Yuan Chen, Yu-Hua Chao, Ju-Pi Li

Background: Patients with idiopathic inflammatory myopathy (IIM) often express a different type of myositis-specific autoantibodies (MSAs), each associated with different clinical symptoms. Understanding the immunopathogenesis of various IIM subgroups can help improve the diagnosis and prognosis of IIM patients with different MSAs. However, the immune cell profiles of these IIM patients with anti-aminoacyl tRNA synthetase (ARS) or anti-melanoma differentiation-associated gene 5 (MDA5) autoantibodies remain unclear. We focused on the immune cell profiles of IIM patients with anti-ARS or anti-MDA5 autoantibodies.

Results: The peripheral blood from IIM patients with anti-MDA5 autoantibody (MDA5 + group, n = 24) or one of the anti-ARS autoantibodies (ARS + group, n = 40) autoantibodies, and healthy controls (HC group, n = 60) were collected and examined. We found that IIM patients had a lower CD3 T cell population compared to the HC group. IIM patients showed a significantly lower TN cell population and a higher TEMRA cell population. Higher Th17 and Treg cell populations were found in these IIM patients than in the HC group. In these IIM patients, the MDA5 + group exhibited the higher percentages of Th17 and Treg cells than the ARS + group. It is noteworthy that the percentage of Th1 cells in the survival subgroup was higher than in the death subgroup in IIM patients with ARS + or MDA5 + . Furthermore, in the MDA5 + group, the percentage of Treg cells was higher in the survival subgroup compared to the death subgroup.

Conclusions: Our study demonstrated that elevated Th1 may be a good prognostic indicator in IIM patients with ARS + or MDA5 + . Elevated Treg may also help predict a good prognosis in MDA5 + IIM patients. However, more large-scale studies and clinical samples are needed to verify the significance of Th1 and Treg cell subsets in clinical outcomes for these IIM patients with ARS + or MDA5 + . These data may help design a therapeutic approach that specifically targets the pathogenic immune molecular responsible for autoimmune attacks in IIM.

背景:特发性炎性肌病(IIM)患者通常表达不同类型的肌炎特异性自身抗体(MSAs),每种抗体都与不同的临床症状有关。了解不同IIM亚组的免疫发病机制有助于改善不同MSAs的IIM患者的诊断和预后。然而,这些具有抗氨酰基tRNA合成酶(ARS)或抗黑色素瘤分化相关基因5(MDA5)自身抗体的IIM患者的免疫细胞图谱尚不清楚。我们重点研究了具有抗ARS或抗MDA5自身抗体的IIM患者的免疫细胞谱。结果:具有抗MDA5自身抗体(MDA5)的IIM患者的外周血 + 组,n = 24)或一种抗ARS自身抗体(ARS + 组,n = 40)自身抗体和健康对照组(HC组,n = 60)进行收集和检查。我们发现,与HC组相比,IIM患者的CD3 T细胞群较低。IIM患者表现出显著较低的TN细胞群和较高的TEMRA细胞群。在这些IIM患者中发现Th17和Treg细胞群高于HC组。在这些IIM患者中,MDA5 + 与ARS相比,该组Th17和Treg细胞的百分比更高 + 组值得注意的是,在患有ARS的IIM患者中,生存亚组中Th1细胞的百分比高于死亡亚组 + 或MDA5 + . 此外,在MDA5中 + Treg细胞在存活亚组中的百分比高于死亡亚组。结论:我们的研究表明,Th1升高可能是伴有ARS的IIM患者的良好预后指标 + 或MDA5 + . Treg升高也可能有助于预测MDA5的良好预后 + IIM患者。然而,需要更大规模的研究和临床样本来验证Th1和Treg细胞亚群在这些患有ARS的IIM患者的临床结果中的意义 + 或MDA5 + . 这些数据可能有助于设计一种治疗方法,专门针对负责IIM自身免疫攻击的致病性免疫分子。
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引用次数: 0
Development and validation of a machine learning-based nomogram for predicting HLA-B27 expression. 用于预测HLA-B27表达的基于机器学习的列线图的开发和验证。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-26 DOI: 10.1186/s12865-023-00566-z
Jichong Zhu, Weiming Tan, Xinli Zhan, Qing Lu, Tuo Liang, JieJiang, Hao Li, Chenxing Zhou, Shaofeng Wu, Tianyou Chen, Yuanlin Yao, Shian Liao, Chaojie Yu, Liyi Chen, Chong Liu

Background: HLA-B27 positivity is normal in patients undergoing rheumatic diseases. The diagnosis of many diseases requires an HLA-B27 examination.

Methods: This study screened totally 1503 patients who underwent HLA-B27 examination, liver/kidney function tests, and complete blood routine examination in First Affiliated Hospital of Guangxi Medical University. The training cohort included 509 cases with HLA-B27 positivity whereas 611 with HLA-B27 negativity. In addition, validation cohort included 147 cases with HLA-B27 positivity whereas 236 with HLA-B27 negativity. In this study, 3 ML approaches, namely, LASSO, support vector machine (SVM) recursive feature elimination and random forest, were adopted for screening feature variables. Subsequently, to acquire the prediction model, the intersection was selected. Finally, differences among 148 cases with HLA-B27 positivity and negativity suffering from ankylosing spondylitis (AS) were investigated.

Results: Six factors, namely red blood cell count, human major compatibility complex, mean platelet volume, albumin/globulin ratio (ALB/GLB), prealbumin, and bicarbonate radical, were chosen with the aim of constructing the diagnostic nomogram using ML methods. For training queue, nomogram curve exhibited the value of area under the curve (AUC) of 0.8254496, and C-value of the model was 0.825. Moreover, nomogram C-value of the validation queue was 0.853, and the AUC value was 0.852675. Furthermore, a significant decrease in the ALB/GLB was noted among cases with HLA-B27 positivity and AS cases.

Conclusion: To conclude, the proposed ML model can effectively predict HLA-B27 and help doctors in the diagnosis of various immune diseases.

背景:HLA-B27阳性在风湿性疾病患者中是正常的。许多疾病的诊断需要进行HLA-B27检查。方法:对广西医科大学第一附属医院1503例患者进行了HLA-B27检测、肝肾功能检查和血常规检查。训练队列包括509例HLA-B27阳性病例,而611例HLA-B28阴性病例。此外,验证队列包括147例HLA-B27阳性病例,而236例HLA-B28阴性病例。本研究采用LASSO、支持向量机递归特征消除和随机森林三种ML方法对特征变量进行筛选。随后,为了获取预测模型,选择交叉点。最后,对148例强直性脊柱炎(AS)HLA-B27阳性和阴性患者的差异进行了研究。结果:选择红细胞计数、人主要相容性复合体、平均血小板体积、白蛋白/球蛋白比(ALB/GLB)、前白蛋白和碳酸氢根6个因素,目的是用ML方法构建诊断列线图。对于训练队列,列线图曲线的曲线下面积(AUC)值为0.8254496,模型的C值为0.825。此外,验证队列的列线图C-值为0.853,AUC值为0.852675。此外,在HLA-B27阳性病例和AS病例中,ALB/GLB显著降低。结论:所提出的ML模型可以有效预测HLA-B27,有助于医生诊断各种免疫性疾病。
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引用次数: 0
Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19. 新冠肺炎患者血清白细胞介素-1β水平与炎症相关基因表达关系的评估。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-18 DOI: 10.1186/s12865-023-00568-x
Zahra Bagheri-Hosseinabadi, Ali Shamsizadeh, Fatemeh Bahrehmand, Mitra Abbasifard

Background: Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients with coronavirus disease 2019 (COVID-19).

Methods: Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA).

Results: The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient's data and expression levels of NLRP1, NLRP3, IL-1β, and ACS.

Conclusions: NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient's condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction.

背景:炎症小体是一组与引起炎症密切相关的分子。本研究旨在评估2019冠状病毒病(新冠肺炎)患者NLR家族pyrin结构域含1(NLRP1)、NLRP3和凋亡相关斑点样蛋白(ASC)的表达及其与血清白细胞介素(IL)-1β水平的关系。采集受试者的外周血样本,通过实时PCR技术评估NLRP1、NLRP3和ASC基因表达。结果:新冠肺炎患者血清IL-1β水平与HS组相比无显著差异。ASC的mRNA表达(P = 0.008)和NLRP1(P = 0.03)基因在新冠肺炎患者中的表达与HS相比显著增加,而研究组之间NLRP3的表达没有显著增加。患者数据与NLRP1、NLRP3、IL-1β和ACS的表达水平之间存在显著相关性。结论:与NLRP3相比,NLRP1和ASC可能在新冠肺炎患者IL-1β活性形式的产生中发挥更关键的作用。然而,患者的血清IL-1β水平没有显著升高,这可能是由于患者的病情以及通过NLRP3表达受损及其功能失常应用病毒逃逸机制所致。
{"title":"Evaluation of the relationship between serum interleukin-1β levels and expression of inflammasome-related genes in patients with COVID-19.","authors":"Zahra Bagheri-Hosseinabadi, Ali Shamsizadeh, Fatemeh Bahrehmand, Mitra Abbasifard","doi":"10.1186/s12865-023-00568-x","DOIUrl":"10.1186/s12865-023-00568-x","url":null,"abstract":"<p><strong>Background: </strong>Inflammasomes are a group of molecules that are strongly involved in causing inflammation. This study aimed to evaluate the expression of NLR family pyrin domain containing 1 (NLRP1), NLRP3, and Apoptosis-associated speck-like protein containing a CARD (ASC) as well as their association with serum level of interleukin (IL)-1β in patients with coronavirus disease 2019 (COVID-19).</p><p><strong>Methods: </strong>Thirty COVID-19 patients and 30 healthy subjects (HS) were recruited. Peripheral blood specimens were collected from subjects to assess NLRP1, NLRP3, and ASC gene expression by Real time-PCR technique. Serum levels of IL-1β were also measured via the enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>The findings showed no significant differences in serum IL-1β level between COVID-19 patients and the HS group. mRNA expression of ASC (P = 0.008) and NLRP1 (P = 0.03) gene had a significant increase in COVID-19 patients compared to HS, while there was no significant increase in the expression of NLRP3 between the studied group. There were significant correlations between patient's data and expression levels of NLRP1, NLRP3, IL-1β, and ACS.</p><p><strong>Conclusions: </strong>NLRP1 and ASC may have a more critical role in the generation of the active form of IL-1β in COVID-19 patients compared to NLRP3. However, serum levels of IL-1β in patients did not show a significant increase, which may be due to the patient's condition and the application of virus escape mechanisms through impaired NLRP3 expression and its malfunction.</p>","PeriodicalId":9040,"journal":{"name":"BMC Immunology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10507843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10314091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of the effect of autoclaved and non-autoclaved live soil exposure on the mouse immune system : Effect of soil exposure on immune system. 高压灭菌和非高压灭菌活土壤暴露对小鼠免疫系统影响的比较:土壤暴露对免疫系统的影响。
IF 3 4区 医学 Q3 Immunology and Microbiology Pub Date : 2023-09-09 DOI: 10.1186/s12865-023-00565-0
Laura Kummola, Martín I González-Rodríguez, Pertti Marnila, Noora Nurminen, Tanja Salomaa, Lotta Hiihtola, Iida Mäkelä, Olli H Laitinen, Heikki Hyöty, Aki Sinkkonen, Ilkka S Junttila

Background: . Lack of exposure to the natural microbial diversity of the environment has been linked to dysregulation of the immune system and numerous noncommunicable diseases, such as allergies and autoimmune disorders. Our previous studies suggest that contact with soil material, rich in naturally occurring microbes, could have a beneficial immunoregulatory impact on the immune system in mice and humans. However, differences in the immunomodulatory properties of autoclaved, sterile soil material and non-autoclaved, live soil material have not been compared earlier.

Results: . In this study, we exposed C57BL/6 mice to autoclaved and live soil powders that had the same rich microbiota before autoclaving. We studied the effect of the soil powders on the mouse immune system by analyzing different immune cell populations, gene expression in the gut, mesenteric lymph nodes and lung, and serum cytokines. Both autoclaved and live soil exposure were associated with changes in the immune system. The exposure to autoclaved soil resulted in higher levels of Rorγt, Inos and Foxp3 expression in the colon. The exposure to live soil was associated with elevated IFN-γ concentration in the serum. In the mesenteric lymph node, exposure to live soil reduced Gata3 and Foxp3 expression, increased the percentage of CD8 + T cells and the expression of activation marker CD80 in XCR1+SIRPα- migratory conventional dendritic cell 1 subset.

Conclusions: . Our results indicate that exposure to the live and autoclaved soil powders is not toxic for mice. Exposure to live soil powder slightly skews the immune system towards type 1 direction which might be beneficial for inhibiting type 2-related inflammation. Further studies are warranted to quantify the impact of this exposure in experimental type 2 inflammation.

背景:。缺乏对环境中自然微生物多样性的接触与免疫系统失调和许多非传染性疾病有关,如过敏和自身免疫性疾病。我们之前的研究表明,与富含天然微生物的土壤物质接触,可能会对小鼠和人类的免疫系统产生有益的免疫调节影响。然而,高压灭菌的无菌土壤材料和非高压灭菌的活土壤材料在免疫调节特性方面的差异尚未更早进行比较。结果:。在这项研究中,我们将C57BL/6小鼠暴露于高压灭菌和活的土壤粉末中,这些粉末在高压灭菌前具有同样丰富的微生物群。我们通过分析不同的免疫细胞群、肠道、肠系膜淋巴结和肺部的基因表达以及血清细胞因子,研究了土粉对小鼠免疫系统的影响。高压灭菌和活土壤暴露都与免疫系统的变化有关。暴露于高压灭菌土壤导致结肠中Rorγt、Inos和Foxp3的表达水平升高。暴露于活土壤与血清中IFN-γ浓度升高有关。在肠系膜淋巴结中,暴露于活土壤降低了Gata3和Foxp3的表达,增加了CD8的百分比 + T细胞和激活标志物CD80在XCR1+SIRPα-迁移的常规树突状细胞1亚群中的表达。结论:。我们的研究结果表明,接触活的和高压灭菌的土壤粉末对小鼠没有毒性。接触活的土壤粉末会使免疫系统向1型方向轻微倾斜,这可能有利于抑制2型相关炎症。需要进一步的研究来量化这种暴露对实验性2型炎症的影响。
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BMC Immunology
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