This study was performed on in vitro bioassay screening for salt tolerance of ten native and six exotic potato genotypes in Bangladesh. Single node was used to evaluate salinity tolerance especially on biomass production. Five different concentrations of NaCl (0 = control, 100, 150, 200 and 250 mM) were used in addition to MS medium and evaluated salt tolerant and sensitive genotype by various morphological and physiological parameters e.g. shoot and root length and its thickness, number of leaves and roots, fresh and dry weight of whole plant and water contents. The ANOVA, DMRT and correlation coefficient were found highly significant at p<0.01 among the genotypes. With salt stress condition highly positive correlation, co-efficient were found between stem length and internodal distance, leaves number, roots number, root length, dry weight of whole plant and fresh weight of whole plant. A dendrogram based on relative values of 10 morphological and physiological parameters of growth under salt conditions were led to clustering into four distinct group’s i.e. tolerant, moderately tolerant, sensitive and very sensitive. On the basis of stress tolerance trait indices (STTIs), Arun (92.78) and Ausha (80.27) showed as a highest salt tolerant, Jamalu (56.33) and Chollisha (57.03) showed the most salt sensitive potato cultivars. From this finding it may be concluded that in vitro screening with bioassay are relatively simple, rapid and convenient and these methods can be used for further advance biotechnological research on potato improvement. J. bio-sci. 28: 21-32, 2020
{"title":"In vitro screening for salt stress tolerance of native and exotic potato genotypes by morphological and physiological parameters","authors":"M. Rashid, S. Islam, M. Bari","doi":"10.3329/jbs.v28i0.44707","DOIUrl":"https://doi.org/10.3329/jbs.v28i0.44707","url":null,"abstract":"This study was performed on in vitro bioassay screening for salt tolerance of ten native and six exotic potato genotypes in Bangladesh. Single node was used to evaluate salinity tolerance especially on biomass production. Five different concentrations of NaCl (0 = control, 100, 150, 200 and 250 mM) were used in addition to MS medium and evaluated salt tolerant and sensitive genotype by various morphological and physiological parameters e.g. shoot and root length and its thickness, number of leaves and roots, fresh and dry weight of whole plant and water contents. The ANOVA, DMRT and correlation coefficient were found highly significant at p<0.01 among the genotypes. With salt stress condition highly positive correlation, co-efficient were found between stem length and internodal distance, leaves number, roots number, root length, dry weight of whole plant and fresh weight of whole plant. A dendrogram based on relative values of 10 morphological and physiological parameters of growth under salt conditions were led to clustering into four distinct group’s i.e. tolerant, moderately tolerant, sensitive and very sensitive. On the basis of stress tolerance trait indices (STTIs), Arun (92.78) and Ausha (80.27) showed as a highest salt tolerant, Jamalu (56.33) and Chollisha (57.03) showed the most salt sensitive potato cultivars. From this finding it may be concluded that in vitro screening with bioassay are relatively simple, rapid and convenient and these methods can be used for further advance biotechnological research on potato improvement. \u0000J. bio-sci. 28: 21-32, 2020","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"28 1","pages":"21-32"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v28i0.44707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44027688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Otomycosis is a chronic ear disease of external auditory canal and it is more prevalent in warm, humid and dusty environment, although it is now found throughout the world. The most commonly found causative agents as of fungal species are Aspergillus and Candida along with preponderance of various fungal and bacterial species has also been reported. The aim of this review is to focus on the prevalence of causal agents of otomycosis in the global context and its clinical management. A total of 63 research articles have been reviewed, which deals prevalence of otomycosis. Its clinicomycological studies both separately and altogether with folk medicine in relation to treatment of otomycosis were also reviewed. Findings of various reports revealed that the Aspergillus and Candida species are the main causal agents of otomycosis. But many reports indicate that a few of other fungal species along with bacteria particularly Staplylococcus aureus and Pseudomonas aeruginosa are also responsible for accelerating this disease in human all over the world. In many reports, improper self-cleaning of ear has been found as major predisposing factor of otomycosis. This review suggests that health education, improvement of socioeconomic status and health caring facilities should be increased for reducing prevalence of otomycosis. However, more research needs to be carried out because of its severe parallel effects to the human health. J. bio-sci. 28: 121-135, 2020
{"title":"Prevalence and Clinicomycological studies of Otomycosis: A review","authors":"KK Nipa, A. Kamal, Ahmed Imtiaj","doi":"10.3329/jbs.v28i0.44718","DOIUrl":"https://doi.org/10.3329/jbs.v28i0.44718","url":null,"abstract":"Otomycosis is a chronic ear disease of external auditory canal and it is more prevalent in warm, humid and dusty environment, although it is now found throughout the world. The most commonly found causative agents as of fungal species are Aspergillus and Candida along with preponderance of various fungal and bacterial species has also been reported. The aim of this review is to focus on the prevalence of causal agents of otomycosis in the global context and its clinical management. A total of 63 research articles have been reviewed, which deals prevalence of otomycosis. Its clinicomycological studies both separately and altogether with folk medicine in relation to treatment of otomycosis were also reviewed. Findings of various reports revealed that the Aspergillus and Candida species are the main causal agents of otomycosis. But many reports indicate that a few of other fungal species along with bacteria particularly Staplylococcus aureus and Pseudomonas aeruginosa are also responsible for accelerating this disease in human all over the world. In many reports, improper self-cleaning of ear has been found as major predisposing factor of otomycosis. This review suggests that health education, improvement of socioeconomic status and health caring facilities should be increased for reducing prevalence of otomycosis. However, more research needs to be carried out because of its severe parallel effects to the human health. \u0000J. bio-sci. 28: 121-135, 2020","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"28 1","pages":"121-135"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v28i0.44718","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41361252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Avilés, A. Barragán, J. Hernández, I. Chóez-Guaranda, R. Viteri, K. Aguaguiña, P. Manzano
This investigation aims to establish the optimum parameters to extract polyphenols from cocoa bean shell (CBS). Time, solvent and method of extraction were considered as factors and Total Content of Polyphenols (TCP) expressed as Gallic Acid Equivalent (GAE) was selected as the response. Results showed that using factorial design the optimum conditions were water in reflux during 5 minutes (262.76 ± 20.66 mg GAE/ 100 g). This revealed that CBS represent a high potential of use in food industry, principally in the field of functional food development.
{"title":"Optimization of polyphenols extraction from cocoa bean shell using factorial design.","authors":"M. Avilés, A. Barragán, J. Hernández, I. Chóez-Guaranda, R. Viteri, K. Aguaguiña, P. Manzano","doi":"10.3329/jbs.v26i0.44664","DOIUrl":"https://doi.org/10.3329/jbs.v26i0.44664","url":null,"abstract":"This investigation aims to establish the optimum parameters to extract polyphenols from cocoa bean shell (CBS). Time, solvent and method of extraction were considered as factors and Total Content of Polyphenols (TCP) expressed as Gallic Acid Equivalent (GAE) was selected as the response. Results showed that using factorial design the optimum conditions were water in reflux during 5 minutes (262.76 ± 20.66 mg GAE/ 100 g). This revealed that CBS represent a high potential of use in food industry, principally in the field of functional food development.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"26 1","pages":"49-54"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v26i0.44664","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46877641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Interphase nuclear phenotype in different accessions (Acc.) of Cynodon dactylon studied in the present experiment showed chromocentric nuclear organization and the chromocenters were found to be visible clearly. The chromocenter numbers were not same and sometimes it was found to be significantly less and never more than total number of chromosomes. Percentages of heterochromatin values were expressed per nuclear area and the values range from 19.759% (Acc. 16) to 66.022% (Acc.18). Nuclear volume as well as interphase chromosome volume was found to vary 0.674 μm3 (Acc.6) to 41.921 μm3 (Acc.10) and from 0.028 μm3 (Acc. 6) to 1.905 μm3 (Acc. 10), respectively. The somatic chromosome number found to vary from 12 to 40. 2n = 18 chromosomes were found in eight accessions of C. dactylon. Only one accession was found to be tetraploid and rest of them aneuploid whose chromosome numbers were 12, 14, 16, 22, 24, 26, 32, 40 etc. The availability of aneuploid shows great aspects of forage breeding programme.
{"title":"Interphase nuclear phenotype and chromosomal variation in Cynodon dactylon (L.) pers","authors":"SK Nitu, S. Islam, H. Tarique","doi":"10.3329/jbs.v27i0.44678","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44678","url":null,"abstract":"Interphase nuclear phenotype in different accessions (Acc.) of Cynodon dactylon studied in the present experiment showed chromocentric nuclear organization and the chromocenters were found to be visible clearly. The chromocenter numbers were not same and sometimes it was found to be significantly less and never more than total number of chromosomes. Percentages of heterochromatin values were expressed per nuclear area and the values range from 19.759% (Acc. 16) to 66.022% (Acc.18). Nuclear volume as well as interphase chromosome volume was found to vary 0.674 μm3 (Acc.6) to 41.921 μm3 (Acc.10) and from 0.028 μm3 (Acc. 6) to 1.905 μm3 (Acc. 10), respectively. The somatic chromosome number found to vary from 12 to 40. 2n = 18 chromosomes were found in eight accessions of C. dactylon. Only one accession was found to be tetraploid and rest of them aneuploid whose chromosome numbers were 12, 14, 16, 22, 24, 26, 32, 40 etc. The availability of aneuploid shows great aspects of forage breeding programme.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"133-141"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44678","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43958948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Oyewole, A. Hamidu, IO Egbewole, R. Adewole, O. Oladoja
This study examined the influence of Escherichia coli on the growth of other selected Gram negative bacteria (Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Pseudomonas aeruginosa and Proteus vulgaris). Cultures of each bacterium at 0, 24, 48 and 72 hours of incubation were plated on MacConkey agar. Colonies that developed were counted while the optical densities were determined at 0, 24, 48 and 72 hours using spectrophotometry. Each bacterium was co-cultured with E. coli and their growth was determined using culturing method and spectrophotometry. The result showed an increase in growth in the cultures of each isolate co-cultured with E. coli when compared with single bacterium culture with the exception of P. aeruginosa. The result of this study revealed a positive growth influence between E. coli and K. pneumoniae, S. dysenteriae, S. typhi, and P. vulgaris, except for P. aeruginosa that showed a decrease in growth.
{"title":"The growth influence of Escherichia coli co-cultured with other selected gram negative bacteria","authors":"O. Oyewole, A. Hamidu, IO Egbewole, R. Adewole, O. Oladoja","doi":"10.3329/jbs.v27i0.44675","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44675","url":null,"abstract":"This study examined the influence of Escherichia coli on the growth of other selected Gram negative bacteria (Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Pseudomonas aeruginosa and Proteus vulgaris). Cultures of each bacterium at 0, 24, 48 and 72 hours of incubation were plated on MacConkey agar. Colonies that developed were counted while the optical densities were determined at 0, 24, 48 and 72 hours using spectrophotometry. Each bacterium was co-cultured with E. coli and their growth was determined using culturing method and spectrophotometry. The result showed an increase in growth in the cultures of each isolate co-cultured with E. coli when compared with single bacterium culture with the exception of P. aeruginosa. The result of this study revealed a positive growth influence between E. coli and K. pneumoniae, S. dysenteriae, S. typhi, and P. vulgaris, except for P. aeruginosa that showed a decrease in growth.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"101-108"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44675","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43596185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbiological investigation has been undertaken for freshly prepared and commercially packed fruit juices of Chittagong city, Bangladesh. Physicochemical analysis as pH, sugar content, total soluble solid (TSS), total dissolved solid (TDS), acidity, moisture content and microbiological parameters i.e. total viable count (TVC), total coliform count (TCC), fecal coliform count (FCC), total staphylococcal count (TSC), Vibrio spp., Salmonella spp., Shigella spp. were examined for all samples. No microorganisms were found positive in packed fruit juice samples, whereas the range of TVC, TCC, and TSC were found to be 2.0 × 103 1.0 × 105, 0.15 × 102 11 × 102 and 1.0 × 102 2.0 × 104 cfu/ml respectively, though there was no detectable FCC and Salmonella, Shigella for vended samples. Human pathogenic Vibrio cholerae, Klebsiella pneumoniae and Staphylococcus aureus were identified by biochemical tests and further confirmed by 16S rDNA sequence analysis. Antibiotic sensitivity of Klebsiella pneumoniae and Staphylococcus aureus were found against ciprofloxacin, imipenem, gentamicin, levofloxacin except amoxicillin; whereas Vibrio cholerae showed resistant to all above antibiotics. Hence, proper awareness and regular monitoring are required during vended fruit juice handling as antibiotic resistant pathogenic bacteria can easily spread through the contaminated fruit juices to the consumers.
{"title":"Microbiological quality assessment of locally vended and commercially packed fruit juices in Chittagong city of Bangladesh","authors":"F. Sultana, L. Marzan, S. Mina","doi":"10.3329/jbs.v27i0.44670","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44670","url":null,"abstract":"Microbiological investigation has been undertaken for freshly prepared and commercially packed fruit juices of Chittagong city, Bangladesh. Physicochemical analysis as pH, sugar content, total soluble solid (TSS), total dissolved solid (TDS), acidity, moisture content and microbiological parameters i.e. total viable count (TVC), total coliform count (TCC), fecal coliform count (FCC), total staphylococcal count (TSC), Vibrio spp., Salmonella spp., Shigella spp. were examined for all samples. No microorganisms were found positive in packed fruit juice samples, whereas the range of TVC, TCC, and TSC were found to be 2.0 × 103 1.0 × 105, 0.15 × 102 11 × 102 and 1.0 × 102 2.0 × 104 cfu/ml respectively, though there was no detectable FCC and Salmonella, Shigella for vended samples. Human pathogenic Vibrio cholerae, Klebsiella pneumoniae and Staphylococcus aureus were identified by biochemical tests and further confirmed by 16S rDNA sequence analysis. Antibiotic sensitivity of Klebsiella pneumoniae and Staphylococcus aureus were found against ciprofloxacin, imipenem, gentamicin, levofloxacin except amoxicillin; whereas Vibrio cholerae showed resistant to all above antibiotics. Hence, proper awareness and regular monitoring are required during vended fruit juice handling as antibiotic resistant pathogenic bacteria can easily spread through the contaminated fruit juices to the consumers.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"43-58"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44670","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44649634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present investigation has been conducted for identification, documentation and exploration of wild edible fruit producing plant species and traditional medicinal uses of these fruits by local peoples from 14 villages of 7 upazila of Barisal district. A total of 43 wild edible fruit producing plant species belonging to 33 genera under 23 families have been recorded. For each species scientific, local and English name, habit, fruiting time and traditional medicinal uses of these fruits have been presented. Among the identified species Polyalthia suberosa Roxb., Flacourtia indica (Burm.f.) Merr., Flacourtia jangomas (Lour.) Rausch., Dimocarpus longan Lour., were recorded from the study area as new findings. Moreover, Flacourtia indica (Burm.f.) Merr., Flacourtia jangomas (Lour.) Rausch., Dimocarpus longan Lour., Polyalthia suberosa Roxb., Syzygium jambos L., Lepisanthes senegalensis (Poir.) Leenhouts., Lepisanthes rubiginosa (Roxb.) Leenh., were identified as rare species and currently they are endangered in their natural habitat. The findings of the research indicate that, there has a great pharmacological value of wild edible fruits for discovering new drugs and medicines.
{"title":"Documentation of wild edible minor fruits used by the local people of Barishal, Bangladesh with emphasis on traditional medicinal values","authors":"A. R. Islam, Sudripta Das, M. Alam, A. Rahman","doi":"10.3329/jbs.v27i0.44672","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44672","url":null,"abstract":"The present investigation has been conducted for identification, documentation and exploration of wild edible fruit producing plant species and traditional medicinal uses of these fruits by local peoples from 14 villages of 7 upazila of Barisal district. A total of 43 wild edible fruit producing plant species belonging to 33 genera under 23 families have been recorded. For each species scientific, local and English name, habit, fruiting time and traditional medicinal uses of these fruits have been presented. Among the identified species Polyalthia suberosa Roxb., Flacourtia indica (Burm.f.) Merr., Flacourtia jangomas (Lour.) Rausch., Dimocarpus longan Lour., were recorded from the study area as new findings. Moreover, Flacourtia indica (Burm.f.) Merr., Flacourtia jangomas (Lour.) Rausch., Dimocarpus longan Lour., Polyalthia suberosa Roxb., Syzygium jambos L., Lepisanthes senegalensis (Poir.) Leenhouts., Lepisanthes rubiginosa (Roxb.) Leenh., were identified as rare species and currently they are endangered in their natural habitat. The findings of the research indicate that, there has a great pharmacological value of wild edible fruits for discovering new drugs and medicines.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"69-81"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Usman An, J. Aliyu, B. Christiana, U. Umar, A. Adedayo, B. David, KF Adamu
Sterile nutrient agar (NA) plates were exposed to the inner and outer environment of the laboratory for 15 minutes before the commencement of laboratory work in the morning and after closure of activities in the evening after which the plates were incubated at 37oC for 24 hours. Antibiotic susceptibility of the bacterial isolates was done using agar well diffusion technique. The aerial bacterial load outside the laboratory was found to be higher than inside the laboratory. Bacteria isolated include members of the genus Staphylococcus, Bacillus, Lactobacillus, Salmonella, Micrococcus, Streptococcus, Proteus and Escherichia. Bacillus subtilis had the highest frequency of occurrence (31.0%) followed by alpha haemolytic streptococcus (19.0%) while the least frequency of occurrence was shown by Proteus bulgaricus and Proteus species (1.7%). All the bacteria were sensitive to antibiotics tested except Micrococcus luteus. They were equally found to be sensitive to the anti-microbial agent (antiseptic and disinfectant) tested except for the hand sanitizer to which most isolates displayed high resistance. The presence of a wide variety of pathogenic microorganisms in the laboratory shows that the environment requires regular cleaning and disinfection to enhance the safety of staff and students using the laboratory. J. bio-sci. 26: 41-48, 2018
{"title":"Isolation and characterization of aerial bacteria from a microbiology laboratory environment","authors":"Usman An, J. Aliyu, B. Christiana, U. Umar, A. Adedayo, B. David, KF Adamu","doi":"10.3329/jbs.v26i0.44663","DOIUrl":"https://doi.org/10.3329/jbs.v26i0.44663","url":null,"abstract":"Sterile nutrient agar (NA) plates were exposed to the inner and outer environment of the laboratory for 15 minutes before the commencement of laboratory work in the morning and after closure of activities in the evening after which the plates were incubated at 37oC for 24 hours. Antibiotic susceptibility of the bacterial isolates was done using agar well diffusion technique. The aerial bacterial load outside the laboratory was found to be higher than inside the laboratory. Bacteria isolated include members of the genus Staphylococcus, Bacillus, Lactobacillus, Salmonella, Micrococcus, Streptococcus, Proteus and Escherichia. Bacillus subtilis had the highest frequency of occurrence (31.0%) followed by alpha haemolytic streptococcus (19.0%) while the least frequency of occurrence was shown by Proteus bulgaricus and Proteus species (1.7%). All the bacteria were sensitive to antibiotics tested except Micrococcus luteus. They were equally found to be sensitive to the anti-microbial agent (antiseptic and disinfectant) tested except for the hand sanitizer to which most isolates displayed high resistance. The presence of a wide variety of pathogenic microorganisms in the laboratory shows that the environment requires regular cleaning and disinfection to enhance the safety of staff and students using the laboratory. \u0000J. bio-sci. 26: 41-48, 2018","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v26i0.44663","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42273671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Girls face discrimination from the moment of her birth. Different underlying factors are responsible for this disparity. Though in recent decades, there is a significant advance in gender disparity, it is still prevalent in different aspect of life throughout the world. Yet a wide level of disparity still exists, whether implicit or explicit, in nutrition and child cares both in the rural and urban areas. Thus, the aim of the study was to determine the gender disparity in nutritional status among under five children in Rajshahi City, Bangladesh. A community based multi-stage cross-sectional study were accomplished for collecting anthropometric and household socio-demographic data of under-five (36≤ age ≤59) children to assess gender disparity on nutritional status in Rajshahi City, Bangladesh. It was performed during the period March 2017 to September 2017. Simple random sampling was used to select children from households by applying multistage sampling technique. Data were analyzed using WHO anthro 2005 software (WHO, Geneva, Switzerland) and Statistical Package for Social Sciences IBM (SPSS) version 22.0. A value of p<0.05 was regarded as statistically significant in the analysis. Under five 371 girls with mean age 47.26 ± 8.33 months and 366 boys with mean age 48.28 ± 7.75 months were participated in this study. No failure in composite anthropometry/normal healthy boys and girls were found 46% and 36% respectively. The prevalence of moderate underweight, stunting and wasting among girls were 23.5%, 27.5% and 14.6% respectively, whereas, among boys 22.7%, 25.4% and 9.8% respectively. Deficiency of energy intake, insufficiency of macro and micro nutrients increase the chance of suffering protein-energy malnutrition, nutritional disorders among girls, compared to boys. Aggregating social development and intervention program of Government may help to reduce gender disparity and improve the nutritional status of overall all children.
{"title":"Gender disparity in nutritional status among under five children in Rajshahi city, Bangladesh","authors":"K. Yeasmin, K. Islam, T. Yeasmin","doi":"10.3329/jbs.v27i0.44666","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44666","url":null,"abstract":"Girls face discrimination from the moment of her birth. Different underlying factors are responsible for this disparity. Though in recent decades, there is a significant advance in gender disparity, it is still prevalent in different aspect of life throughout the world. Yet a wide level of disparity still exists, whether implicit or explicit, in nutrition and child cares both in the rural and urban areas. Thus, the aim of the study was to determine the gender disparity in nutritional status among under five children in Rajshahi City, Bangladesh. A community based multi-stage cross-sectional study were accomplished for collecting anthropometric and household socio-demographic data of under-five (36≤ age ≤59) children to assess gender disparity on nutritional status in Rajshahi City, Bangladesh. It was performed during the period March 2017 to September 2017. Simple random sampling was used to select children from households by applying multistage sampling technique. Data were analyzed using WHO anthro 2005 software (WHO, Geneva, Switzerland) and Statistical Package for Social Sciences IBM (SPSS) version 22.0. A value of p<0.05 was regarded as statistically significant in the analysis. Under five 371 girls with mean age 47.26 ± 8.33 months and 366 boys with mean age 48.28 ± 7.75 months were participated in this study. No failure in composite anthropometry/normal healthy boys and girls were found 46% and 36% respectively. The prevalence of moderate underweight, stunting and wasting among girls were 23.5%, 27.5% and 14.6% respectively, whereas, among boys 22.7%, 25.4% and 9.8% respectively. Deficiency of energy intake, insufficiency of macro and micro nutrients increase the chance of suffering protein-energy malnutrition, nutritional disorders among girls, compared to boys. Aggregating social development and intervention program of Government may help to reduce gender disparity and improve the nutritional status of overall all children.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44666","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44262202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes.
本研究旨在利用从孟加拉国加齐浦尔BARI收集的孟加拉国大麦基因型，改进离体愈伤组织诱导和植株再生的有效方案。将6种大麦基因型(BB-1、BB-2、BB-3、BB-4、BB-5和BB-6)的成熟种子精心分离胚，在添加不同浓度和组合的pgr的MS培养基中培养愈伤组织诱导和再生。6个基因型中，MS + 4.0 mg/l 2,4- d + 200 mg/l l -脯氨酸+ 300 mg/l酪蛋白水解物对BB-6的愈伤组织诱导率最高(38.17%);而BB-4和BB-5在同一培养基中未诱导出愈伤组织。在添加1.5 mg/l BAP + 30 g/l蔗糖的MS培养基中，同一基因型(BB-6)愈伤组织的植株再生效果最好(19.25%)。方差分析(ANOVA)显示培养基和基因型之间存在极显著差异。
{"title":"An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)","authors":"M. Haque, S. Islam","doi":"10.3329/jbs.v27i0.44674","DOIUrl":"https://doi.org/10.3329/jbs.v27i0.44674","url":null,"abstract":"This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes.","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"27 1","pages":"89-99"},"PeriodicalIF":0.0,"publicationDate":"2019-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3329/jbs.v27i0.44674","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48083417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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