Lathyrus sativus known as grass pea belongs to the family of Fabaceae commonly grown for human consumption and livestock feed throughout the world. This study based on the morphological features of the basic chromosome numbers and numerical characterization of the karyotypes using total chromosome length (TCL), arm ratio and total fraction frequency. Outcome of BARI Khesari-2 is more effective than BARI Khesari-3 on the basis of mitotic index, nuclear volume and interphase chromosome volume. Whereas, mitotic index directly relevant to cell division; when MI high than cell division rate will be high. The two varieties (BARI Khesari-2 and BARI Khesari-3) were found to possess 2n = 14 chromosomes. Karyotype analysis is an essential tool for studying the genetic constitution of any organism. In this investigation, we found the karyotype formula of BARI Khesari-2 is 7L 4m + 2sm + 1st and BARI Khesari-3 is 7L 2m +3sm + 2st. Chromosome morphology showed a variation in the chromosome length, chromosome structure and centromere position of the accessions. Presence of sub-terminal chromosome in both varieties shows asymmetric karyotype which indicates advanceness. The variation in L. sativus may be regarded as having evolution and adaptive significance. J. Bio-Sci. 30(2): 59-65, 2022
{"title":"Nuclear Phenotype and Karyotype Analysis in two Varieties of Lathyrus Sativus L.","authors":"Md Saifur Rahman, Ummay Salma Pritha, Shaila Ferdous, Md Mamunur Rashid Sarkar, Golam Kabir","doi":"10.3329/jbs.v30i2.68913","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68913","url":null,"abstract":"Lathyrus sativus known as grass pea belongs to the family of Fabaceae commonly grown for human consumption and livestock feed throughout the world. This study based on the morphological features of the basic chromosome numbers and numerical characterization of the karyotypes using total chromosome length (TCL), arm ratio and total fraction frequency. Outcome of BARI Khesari-2 is more effective than BARI Khesari-3 on the basis of mitotic index, nuclear volume and interphase chromosome volume. Whereas, mitotic index directly relevant to cell division; when MI high than cell division rate will be high. The two varieties (BARI Khesari-2 and BARI Khesari-3) were found to possess 2n = 14 chromosomes. Karyotype analysis is an essential tool for studying the genetic constitution of any organism. In this investigation, we found the karyotype formula of BARI Khesari-2 is 7L 4m + 2sm + 1st and BARI Khesari-3 is 7L 2m +3sm + 2st. Chromosome morphology showed a variation in the chromosome length, chromosome structure and centromere position of the accessions. Presence of sub-terminal chromosome in both varieties shows asymmetric karyotype which indicates advanceness. The variation in L. sativus may be regarded as having evolution and adaptive significance. J. Bio-Sci. 30(2): 59-65, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135816891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of the study was to screen phytochemicals and assess the antioxidant as well as anti-inflammatory properties of Vitex pinnata leaf extracts. Alkaloids, glycosides, tannins, terpenoids, saponins, phlobatannins, steroids, quinine, phenol, coumarins, and flavonoids were found during the phytochemical investigation. V. pinnata leaf extract exhibited an IC50 value of 154.33 µg/ml in the DPPH free radical scavenging assay (standard: IC50 = 25.48 µg/ml) IC50 value of 218.8 µg/ml in the albumin denaturation inhibition assay (standard: IC50 = 33.74 µg/ml). According to the findings, the leaves of V. pinnata contain a variety of pharmaceutically important secondary metabolites capable of scavenging DPPH and inhibiting albumin denaturation, demonstrating their antioxidant and anti-inflammatory properties. J. Bio-Sci. 30(2): 01-11, 2022
{"title":"Qualitative Phytochemical Screening, Evaluation of Antioxidant and Anti-Inflammatory Potentials of Vitex pinnata L. Leaf Extract","authors":"Najmun Naher Sonia, Minhajur Rahman, Animesh Biswas","doi":"10.3329/jbs.v30i2.68905","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68905","url":null,"abstract":"The purpose of the study was to screen phytochemicals and assess the antioxidant as well as anti-inflammatory properties of Vitex pinnata leaf extracts. Alkaloids, glycosides, tannins, terpenoids, saponins, phlobatannins, steroids, quinine, phenol, coumarins, and flavonoids were found during the phytochemical investigation. V. pinnata leaf extract exhibited an IC50 value of 154.33 µg/ml in the DPPH free radical scavenging assay (standard: IC50 = 25.48 µg/ml) IC50 value of 218.8 µg/ml in the albumin denaturation inhibition assay (standard: IC50 = 33.74 µg/ml). According to the findings, the leaves of V. pinnata contain a variety of pharmaceutically important secondary metabolites capable of scavenging DPPH and inhibiting albumin denaturation, demonstrating their antioxidant and anti-inflammatory properties. J. Bio-Sci. 30(2): 01-11, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Saifullah Bin Aziz, Md Mehedi Alam, Mohammad Mahfujul Haque, Md Mostafizur Rahman Mondol
A study was conducted to examine the various factors influencing the decline in fish production in the Hakaluki Haor wetland ecosystem in northeast Bangladesh, aiming to investigate the impacts of both climatic and anthropogenic factors on fish production in the area. Nowadays, this wetland is increasingly impacted by climate change, which poses significant challenges to fish production and sustainability. The primary data were collected through a face-to-face questionnaire survey (n = 150) with fishers, focus group discussion (n =15), and key informant interviews (n = 10). The majority of the fishers were middle-aged group (31-50 year), whereas 41% had primary level (schooling year 5) of education and 48% were highly experienced fishermen (≤16 years). Stakeholder perceptions of human activities, including overfishing (52%), discard of non-target species (23%), use of destructive fishing gear (67%), beel drying (47%), habitat destruction (48%), water pollution (23%), migration route changes (30%) and agricultural intensification (45%), have been identified to reduce fish production. Additionally, the study revealed that climate change impacts the ecosystem, including temperature fluctuation (57%), erratic rainfall (49%), flood and flash flood (37%), drought (17%), and siltation in beel (37%). Stakeholders perceived these impacts to have significant negative effects on aquatic biodiversity, particularly in terms of habitat destruction and water quality degradation. This study proposes potential solutions to mitigate the impacts of climate changes and man-made factors associated with fish production reduction in the region, such as improved water management practices, diversification of fish species, and the use of climate-resilient fisheries management. Community engagement and government support are highlighted as crucial factors to address the impacts of climate change on fish production in Hakaluki Haor. The findings of this study have important implications for the development of effective management strategies for ensuring sustainable livelihoods for fishers. J. Bio-Sci. 30(2): 23-37, 2022
{"title":"Reduction of Fish Production in Hakaluki Haor Caused by Climatic and Anthropogenic Factors","authors":"Md Saifullah Bin Aziz, Md Mehedi Alam, Mohammad Mahfujul Haque, Md Mostafizur Rahman Mondol","doi":"10.3329/jbs.v30i2.68908","DOIUrl":"https://doi.org/10.3329/jbs.v30i2.68908","url":null,"abstract":"A study was conducted to examine the various factors influencing the decline in fish production in the Hakaluki Haor wetland ecosystem in northeast Bangladesh, aiming to investigate the impacts of both climatic and anthropogenic factors on fish production in the area. Nowadays, this wetland is increasingly impacted by climate change, which poses significant challenges to fish production and sustainability. The primary data were collected through a face-to-face questionnaire survey (n = 150) with fishers, focus group discussion (n =15), and key informant interviews (n = 10). The majority of the fishers were middle-aged group (31-50 year), whereas 41% had primary level (schooling year 5) of education and 48% were highly experienced fishermen (≤16 years). Stakeholder perceptions of human activities, including overfishing (52%), discard of non-target species (23%), use of destructive fishing gear (67%), beel drying (47%), habitat destruction (48%), water pollution (23%), migration route changes (30%) and agricultural intensification (45%), have been identified to reduce fish production. Additionally, the study revealed that climate change impacts the ecosystem, including temperature fluctuation (57%), erratic rainfall (49%), flood and flash flood (37%), drought (17%), and siltation in beel (37%). Stakeholders perceived these impacts to have significant negative effects on aquatic biodiversity, particularly in terms of habitat destruction and water quality degradation. This study proposes potential solutions to mitigate the impacts of climate changes and man-made factors associated with fish production reduction in the region, such as improved water management practices, diversification of fish species, and the use of climate-resilient fisheries management. Community engagement and government support are highlighted as crucial factors to address the impacts of climate change on fish production in Hakaluki Haor. The findings of this study have important implications for the development of effective management strategies for ensuring sustainable livelihoods for fishers. J. Bio-Sci. 30(2): 23-37, 2022","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hosen, M. Hoque, M. Rahim, A. Hannan, T. Biswas, K. Akter
Tomato (Solanum lycopersicum L.) is one of the most popular vegetable crops in Bangladesh. The production of this crop in open field is constrained by major pests like fruit borer, yellow striped armyworm and caterpillar. A pest exclusion net (PEN) experiment was carried out to assess the performance of seven varieties of tomato through growth, yield and pest occurrence. The two-factor experiment was carried out in randomized complete block design (RCBD) with three conditions viz. under net without pesticide, no net with pesticide, no net without pesticide and three replications. Data were analyzed with MSTAT-Cv. 2.1 software package and means were separated by Duncan’s multiple range test (DMRT) at 1% level of significance. The result revealed that crop under PEN had maximum plant height (98.47±1.02 cm), inflorescence number per plant (20.34±0.06), open flowers number per plant (69.34±2.02), fruit number per plant (43.12±0.03), individual fruit weight (49.70±0.02 g), gross yield (50.76±1.06 tons/ha) whereas no net with or without pesticide condition produced the minimum above traits. However, most of the parameters showed decreasing trend under no net condition except pest incidence. This study shows that the use of PEN protect tomato against pests, increases growth and yield which can be considered as a viable technology for tomato production by marginal farmers of Bangladesh. J Bio-Sci. 30(1): 21-32, 2022 (June)
{"title":"Growth, Yield and Pest Infestation Assessment of Solanum Lycopersicum Under Pest Exclusion Net (Pen) Technique in Bangladesh","authors":"M. Hosen, M. Hoque, M. Rahim, A. Hannan, T. Biswas, K. Akter","doi":"10.3329/jbs.v30i1.63098","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63098","url":null,"abstract":"Tomato (Solanum lycopersicum L.) is one of the most popular vegetable crops in Bangladesh. The production of this crop in open field is constrained by major pests like fruit borer, yellow striped armyworm and caterpillar. A pest exclusion net (PEN) experiment was carried out to assess the performance of seven varieties of tomato through growth, yield and pest occurrence. The two-factor experiment was carried out in randomized complete block design (RCBD) with three conditions viz. under net without pesticide, no net with pesticide, no net without pesticide and three replications. Data were analyzed with MSTAT-Cv. 2.1 software package and means were separated by Duncan’s multiple range test (DMRT) at 1% level of significance. The result revealed that crop under PEN had maximum plant height (98.47±1.02 cm), inflorescence number per plant (20.34±0.06), open flowers number per plant (69.34±2.02), fruit number per plant (43.12±0.03), individual fruit weight (49.70±0.02 g), gross yield (50.76±1.06 tons/ha) whereas no net with or without pesticide condition produced the minimum above traits. However, most of the parameters showed decreasing trend under no net condition except pest incidence. This study shows that the use of PEN protect tomato against pests, increases growth and yield which can be considered as a viable technology for tomato production by marginal farmers of Bangladesh.\u0000J Bio-Sci. 30(1): 21-32, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49323161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Azad, Mithun Adhikari, Md. Nuruzzaman Sarker, Anm Maruf Hasan, M. N. Amin
An efficient protocol for in vitro clonal propagation of Smilax zeylanica was developed using nodal and shoot tip segments from field grown plants. Nodal explants performed better than shoot tip explants when two distinct explants were tested for shoot proliferation. The best results for shoot proliferation were achieved with MS medium supplemented with BAP and Kn alone or in combination with NAA and IBA at various concentrations. On MS medium containing 4.0 μM BAP, the greatest number of shoots were produced. In vitro proliferating shoots were transplanted to full strength MS medium fortified with IBA and NAA separately at varying concentrations (1.0 - 6.0 μM) in order to induce adventitious roots. The medium containing 2.0 μM IBA showed the best rooting reactions. Plantlets having well developed root system were transferred to soil and successfully acclimatized with 80% survival rate under ex vitro condition. J. Bio-Sci. 30(1): 69-78, 2022 (June)
{"title":"In Vitro Shoot Regeneration From Nodal and Shoot Tip Explants of a Threatened Medicinal Plant - Smilax Zeylanica L.","authors":"M. Azad, Mithun Adhikari, Md. Nuruzzaman Sarker, Anm Maruf Hasan, M. N. Amin","doi":"10.3329/jbs.v30i1.63102","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63102","url":null,"abstract":"An efficient protocol for in vitro clonal propagation of Smilax zeylanica was developed using nodal and shoot tip segments from field grown plants. Nodal explants performed better than shoot tip explants when two distinct explants were tested for shoot proliferation. The best results for shoot proliferation were achieved with MS medium supplemented with BAP and Kn alone or in combination with NAA and IBA at various concentrations. On MS medium containing 4.0 μM BAP, the greatest number of shoots were produced. In vitro proliferating shoots were transplanted to full strength MS medium fortified with IBA and NAA separately at varying concentrations (1.0 - 6.0 μM) in order to induce adventitious roots. The medium containing 2.0 μM IBA showed the best rooting reactions. Plantlets having well developed root system were transferred to soil and successfully acclimatized with 80% survival rate under ex vitro condition.\u0000J. Bio-Sci. 30(1): 69-78, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42201985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluated the growth and reproductive activity of Thai sharpunti Barbonymus gonionotus in the L-shaped freshwater pond at Rajshahi University campus of northwest Bangladesh during January and August, 2017. Length and weight increment was recorded for the growth study and gonado-somatic index (GSI) with fecundity was estimated for the assessment of reproductive activity. During the study a significant length and weight increment was observed (p<0.05). Monthly mean total length (TL) ranged 11.76 to 22.47 cm, fork length (FL) was 10.02 to 19.12 cm, standard length (SL) was 8.87 to 16.83 cm and body weight (BW) was 19.89 to 167.81g. Both the length-length relationship (LLR) and length-weight relationship (LWR) showed a positive allometric growth pattern of the fish and the relationships were TL = 1.1759 FL - 0.035, TL = 1.338 SL - 0.0002, FL = 1.0988 SL + 0.6557 and BW = 0.0051TL 3.347. Gonadal observation showed that the fish initiated sexuality in the month of April and was fully matured in the month of May. During the study male female ratio was significantly different from the 1:1 ratio (χ2 = 3.84, p<0.05) and the overall male to female sex ratio was 1:1.42. The gonado-somatic index (GSI) was ranged from 3.72 to 32.39 and showed significant monthly fluctuations (p<0.05). A sharp decline of GSI during May and June indicates the peak spawning of B. gonionotus in the study site. During the study the fecundity of the fish was ranged from 46145 to 353969 and the highest fecundity was recorded in the months of May. The findings of the present study would be helpful for the sustainable management of B. gonionotus in the freshwater ecosystem of Bangladesh and elsewhere. J Bio-Sci. 30(1): 101-114, 2022 (June)
{"title":"Growth and Reproduction of Thai Sharpunti (Barbonymus Gonionotus) In the Lentic Water Habitat of Northwest Bangladesh","authors":"MR Ali, M. Mondol","doi":"10.3329/jbs.v30i1.63105","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63105","url":null,"abstract":"This study evaluated the growth and reproductive activity of Thai sharpunti Barbonymus gonionotus in the L-shaped freshwater pond at Rajshahi University campus of northwest Bangladesh during January and August, 2017. Length and weight increment was recorded for the growth study and gonado-somatic index (GSI) with fecundity was estimated for the assessment of reproductive activity. During the study a significant length and weight increment was observed (p<0.05). Monthly mean total length (TL) ranged 11.76 to 22.47 cm, fork length (FL) was 10.02 to 19.12 cm, standard length (SL) was 8.87 to 16.83 cm and body weight (BW) was 19.89 to 167.81g. Both the length-length relationship (LLR) and length-weight relationship (LWR) showed a positive allometric growth pattern of the fish and the relationships were TL = 1.1759 FL - 0.035, TL = 1.338 SL - 0.0002, FL = 1.0988 SL + 0.6557 and BW = 0.0051TL 3.347. Gonadal observation showed that the fish initiated sexuality in the month of April and was fully matured in the month of May. During the study male female ratio was significantly different from the 1:1 ratio (χ2 = 3.84, p<0.05) and the overall male to female sex ratio was 1:1.42. The gonado-somatic index (GSI) was ranged from 3.72 to 32.39 and showed significant monthly fluctuations (p<0.05). A sharp decline of GSI during May and June indicates the peak spawning of B. gonionotus in the study site. During the study the fecundity of the fish was ranged from 46145 to 353969 and the highest fecundity was recorded in the months of May. The findings of the present study would be helpful for the sustainable management of B. gonionotus in the freshwater ecosystem of Bangladesh and elsewhere.\u0000J Bio-Sci. 30(1): 101-114, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43021712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was conducted to assess the features of the stinging catfish Heteropneustes fossilis (Bloch) in terms of total length (TL), total weight (TW) and growth performance and survival rate after treatment of fish feed with a synthetic male hormone, 17 α-methyltestosterone (17 α-MT) under laboratory conditions. Results showed that TL in the untreated (C) and T1 group of fishes differed significantly (p<0.05) even though the overall effect of the hormone did not influence TL of the fishes under study (p = 0.085). A similar result was observed for TW, but the overall impact of 17-αMT on TW of H. fossilis was highly significant one (p<0.001), indicating that the TW was increased much more than the TL due to the hormone treatment in the experimental fishes. Growth performances of the fishes in terms of their gains in TL and TW due to the hormone treatment and over a rearing period of four months from March to June showed that compared to the control groups, all three treatment groups increased in both the morphometric parameters in H. fossilis. The increases in TW were more drastic than the corresponding increases in TL. Notably, the hormone treatments did not affect the survival of the experiment fishes under study. Histopathological changes in the intestine of T2 fishes were characterized by hemorrhage and degeneration of villi, whereas hemorrhage, degeneration of serosa and rapture of villi were remarkable in T3 group. Enlarged glomeruli, lots of vacuoles, disorganization of renal tubules and necrosis were observed in T2 whereas the T3 group was characterized by vacuoles, disorganization of tubules, enlarging glomeruli and wider tubular lumen. Since the present results clearly demonstrated that 17 α-MT treatments enhanced the total length and weight as well as growth performance of H. fossilis, carefully designed experiments with appropriate doses of the hormone in fish feed would be beneficial for the commercial production and conservation of this popular catfish in the country. J Bio-Sci. 30(1): 131-141, 2022 (June)
{"title":"A Male Hormone-Induced Changes in Growth Performance and Histopathology in the Stinging Catfish Heteropneustes Fossilis (Siluriformes: Heteropneustidae) Under Laboratory Conditions","authors":"S. Sultana, Fahmida Farha","doi":"10.3329/jbs.v30i1.63108","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63108","url":null,"abstract":"The present study was conducted to assess the features of the stinging catfish Heteropneustes fossilis (Bloch) in terms of total length (TL), total weight (TW) and growth performance and survival rate after treatment of fish feed with a synthetic male hormone, 17 α-methyltestosterone (17 α-MT) under laboratory conditions. Results showed that TL in the untreated (C) and T1 group of fishes differed significantly (p<0.05) even though the overall effect of the hormone did not influence TL of the fishes under study (p = 0.085). A similar result was observed for TW, but the overall impact of 17-αMT on TW of H. fossilis was highly significant one (p<0.001), indicating that the TW was increased much more than the TL due to the hormone treatment in the experimental fishes. Growth performances of the fishes in terms of their gains in TL and TW due to the hormone treatment and over a rearing period of four months from March to June showed that compared to the control groups, all three treatment groups increased in both the morphometric parameters in H. fossilis. The increases in TW were more drastic than the corresponding increases in TL. Notably, the hormone treatments did not affect the survival of the experiment fishes under study. Histopathological changes in the intestine of T2 fishes were characterized by hemorrhage and degeneration of villi, whereas hemorrhage, degeneration of serosa and rapture of villi were remarkable in T3 group. Enlarged glomeruli, lots of vacuoles, disorganization of renal tubules and necrosis were observed in T2 whereas the T3 group was characterized by vacuoles, disorganization of tubules, enlarging glomeruli and wider tubular lumen. Since the present results clearly demonstrated that 17 α-MT treatments enhanced the total length and weight as well as growth performance of H. fossilis, carefully designed experiments with appropriate doses of the hormone in fish feed would be beneficial for the commercial production and conservation of this popular catfish in the country.\u0000J Bio-Sci. 30(1): 131-141, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Toukir Mahmud Asif, M. Nesa, Kazi Mahnaz Mehrin, Nelufa Yasmin, A. Islam, N. Fatema, N. Islam
Petroleum ether (PE), chloroform (CHCl3), and methanol (CH3OH) extracts of the whole chickpea plant Cicer arietinum (L.) have been tested against adult beetles of Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) through dose-mortality and repellent activity tests. In dose mortality tests, the PE extracts of C. arietinum gave LD50 values of 3.466, 3.292, 2.721, 2.398, 1.887, 1.663 and 1.428 mg/cm2 against C. chinensis and 4.297, 3.415, 2.246, 1.725, 1.384, 0.984 and 0.722 mg/cm2 against S. oryzae both after 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. The CHCl3 extracts gave LD50 values of 5.242, 4.844, 3.472, 2.945, 2.540, 1.875, 1.295 and 1.053 mg/cm2 against C. chinensis after 6, 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. The CH3OH extracts, on the other hand, yielded LD50 values of 0.923, 0.757, 0.649, 0.515, 0.400, 0.303, 0.247 and 0.197 mg/cm2 against C. chinensis, 4.227, 3.528, 3.454, 2.933, 2.493, 2.187, 1.938 and 1.724 mg/cm2 against S. oryzae and 3.485, 3.176, 2.851, 1.531, 1.113, 0.756, 0.534 and 0.478 mg/cm2 against T. castaneum adults after 6, 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. However, the PE extracts of C. arietinum did not show any mortality against T. castaneum and the CHCl3 extracts were inactive against S. oryzae and T. castaneum adults. In repellency tests, all three organic extracts did not show any repellency against the test beetles. According to their intensity of activities, the extracts could be arranged in the following descending order: CH3OH extracts against C. chinensis > CH3OH extracts against T. castaneum > PE extracts against S. oryzae > CHCl3 extracts against C. chinensis > PE extracts against C. chinensis > CH3OH extracts against S. oryzae adults. The present results indicate that the test plant C. arietinum contains bioactive compounds and could be used as a source of control agents for the stored product pests. J Bio-Sci. 30(1): 91-100, 2022 (June)
{"title":"Screening for Insecticidal Potential of the Chickpea Plant Cicer Arietinum (L.) Against Three Stored Grain Pests Callosobruchus Chinensis (L.), Sitophilus Oryzae (L.) And Tribolium Castaneum (Herbst) Adults Under Laboratory Conditions","authors":"Toukir Mahmud Asif, M. Nesa, Kazi Mahnaz Mehrin, Nelufa Yasmin, A. Islam, N. Fatema, N. Islam","doi":"10.3329/jbs.v30i1.63104","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63104","url":null,"abstract":"Petroleum ether (PE), chloroform (CHCl3), and methanol (CH3OH) extracts of the whole chickpea plant Cicer arietinum (L.) have been tested against adult beetles of Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) through dose-mortality and repellent activity tests. In dose mortality tests, the PE extracts of C. arietinum gave LD50 values of 3.466, 3.292, 2.721, 2.398, 1.887, 1.663 and 1.428 mg/cm2 against C. chinensis and 4.297, 3.415, 2.246, 1.725, 1.384, 0.984 and 0.722 mg/cm2 against S. oryzae both after 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. The CHCl3 extracts gave LD50 values of 5.242, 4.844, 3.472, 2.945, 2.540, 1.875, 1.295 and 1.053 mg/cm2 against C. chinensis after 6, 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. The CH3OH extracts, on the other hand, yielded LD50 values of 0.923, 0.757, 0.649, 0.515, 0.400, 0.303, 0.247 and 0.197 mg/cm2 against C. chinensis, 4.227, 3.528, 3.454, 2.933, 2.493, 2.187, 1.938 and 1.724 mg/cm2 against S. oryzae and 3.485, 3.176, 2.851, 1.531, 1.113, 0.756, 0.534 and 0.478 mg/cm2 against T. castaneum adults after 6, 12, 18, 24, 30, 36, 42 and 48 h of exposures, respectively. However, the PE extracts of C. arietinum did not show any mortality against T. castaneum and the CHCl3 extracts were inactive against S. oryzae and T. castaneum adults. In repellency tests, all three organic extracts did not show any repellency against the test beetles. According to their intensity of activities, the extracts could be arranged in the following descending order: CH3OH extracts against C. chinensis > CH3OH extracts against T. castaneum > PE extracts against S. oryzae > CHCl3 extracts against C. chinensis > PE extracts against C. chinensis > CH3OH extracts against S. oryzae adults. The present results indicate that the test plant C. arietinum contains bioactive compounds and could be used as a source of control agents for the stored product pests.\u0000J Bio-Sci. 30(1): 91-100, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45956075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Nahid Islam Chowdhury, Md. Akram Ullah, M. B. Hossain, SM Shahinul Islam
Congenital heart disease (CHD) is a congenital defect affecting the structure of the heart. Early detection can be done by auscultating the murmur and this could help to diagnosis the disease. This study was aimed to detect CHD by rapid evaluation of clinical features, findings and cardiac auscultation which will help to customize the future management plan in each CHD child and reduce the progression of complications and financial burden associated with CHD on this region over time. This descriptive cross-sectional study was conducted on children hospitalized to the paediatric department. A systematic sampling technique was implemented. Total 3978 children were taken during the study period among them 54 were detected as CHD. Total 3978 children under 10 years were examined during the study. Among them-murmur with CHD was found in 54 (1.36%) cases and it was found that 30 (55.6%) were male and most (53.7%) of them from rural area came from 42.6% middle socio-economic group. It was revealed that, among the study cases 40.70% had ventricular septal defect (VSD), 63% had rapid breathing regarding clinical presentation. Regarding clinical findings among the study cases 33 (61.10%) had chest retractions. Among 54 murmur positive cases 29 (53.70%) had pansystolic murmur which help to assess regarding CHD before completing echo. Congenital heart disease, especially in the newborn era, is a pediatric cardiology emergency due to the high morbidity and mortality rates. This research could aid in the early diagnosis of CHD and save the lives of youngsters. J Bio-Sci. 30(1): 115-121, 2022 (June)
{"title":"Prevalence and Clinical Features of Cardiac Murmur With Its Subtypes of Congenital Heart Disease in Rajshahi, Bangladesh","authors":"Md Nahid Islam Chowdhury, Md. Akram Ullah, M. B. Hossain, SM Shahinul Islam","doi":"10.3329/jbs.v30i1.63106","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63106","url":null,"abstract":"Congenital heart disease (CHD) is a congenital defect affecting the structure of the heart. Early detection can be done by auscultating the murmur and this could help to diagnosis the disease. This study was aimed to detect CHD by rapid evaluation of clinical features, findings and cardiac auscultation which will help to customize the future management plan in each CHD child and reduce the progression of complications and financial burden associated with CHD on this region over time. This descriptive cross-sectional study was conducted on children hospitalized to the paediatric department. A systematic sampling technique was implemented. Total 3978 children were taken during the study period among them 54 were detected as CHD. Total 3978 children under 10 years were examined during the study. Among them-murmur with CHD was found in 54 (1.36%) cases and it was found that 30 (55.6%) were male and most (53.7%) of them from rural area came from 42.6% middle socio-economic group. It was revealed that, among the study cases 40.70% had ventricular septal defect (VSD), 63% had rapid breathing regarding clinical presentation. Regarding clinical findings among the study cases 33 (61.10%) had chest retractions. Among 54 murmur positive cases 29 (53.70%) had pansystolic murmur which help to assess regarding CHD before completing echo. Congenital heart disease, especially in the newborn era, is a pediatric cardiology emergency due to the high morbidity and mortality rates. This research could aid in the early diagnosis of CHD and save the lives of youngsters.\u0000J Bio-Sci. 30(1): 115-121, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48182814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coronavirus disease 2019 (COVID-19) pandemic represents a massive impact on human health, causing sudden lifestyle changes, through social distancing and isolation at home, with social and economic consequences. The aim of the study was to determine both of community and gender-based distribution of perception, attitude, preventive measures, changing lifestyle regarding COVID-19 and to help the Government and policymakers to take indispensable steps. This cross-sectional survey self-administered online-based study was carried out after the declaration of strict initial lockdown enforced in Bangladesh. The study was conducted in Rajshahi district from 30 March 2020 to 31 August 2020, a very critical time of mental suffering and lockdown. A total of 651 respondents from Rajshahi district in Bangladesh participated in the survey, and by a self-administered questionnaire, designed in the language of English. Among the collected responses, 535 were considered for the analysis, which largely belonged to Rajshahi’s middle to upper socio-economic status (SES). The data analysis was performed using Microsoft Excel 2019 and SPSS version 25.0 (Chicago, IL, USA). The data were analyzed by using descriptive statistic, chi-square test and z-proportional test. In this empirical study, 278 males and 257 females participated. More than one-tenth of males and 35% of females were not conscious of the type of Coronavirus. A lot of misperception about the incubation period of COVID-19 existed among participants. The research observed that 34.2%, of males and 25.3% of the female study population had no opinion about the treatment of COVID-19 and 27.3% of males and 21.8% of females gave a verdict that COVID-19 had no available treatment. The study discovered more surprisingly that females (67.3%), greater than males (65.5%) did not want to stay at home during the lockdown. Positively 87.8% of males and 70.4% of females washed their hands with soap or used sanitizer after coming back from outside. The consumption of a balanced diet increased by males and females was 13.3% and 6.7% respectively. Z-proportional test showed that regarding COVID-19, there were significant difference between male and female’s conception and precaution (p<0.0001). The study has used the first-time data based on the population’s lifestyle and eating habits during the COVID-19 lockdown, which will give assistance to the policy makers to develop the situation. J Bio-Sci. 30(1): 33-48, 2022 (June)
{"title":"Lifestyle and Immunity Boosting Practices During COVID-19 Pandemic Among the Population of Rajshahi District, Bangladesh","authors":"K. Yeasmin, Mam Samaun, H. Jahan, T. Yesmin","doi":"10.3329/jbs.v30i1.63099","DOIUrl":"https://doi.org/10.3329/jbs.v30i1.63099","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) pandemic represents a massive impact on human health, causing sudden lifestyle changes, through social distancing and isolation at home, with social and economic consequences. The aim of the study was to determine both of community and gender-based distribution of perception, attitude, preventive measures, changing lifestyle regarding COVID-19 and to help the Government and policymakers to take indispensable steps. This cross-sectional survey self-administered online-based study was carried out after the declaration of strict initial lockdown enforced in Bangladesh. The study was conducted in Rajshahi district from 30 March 2020 to 31 August 2020, a very critical time of mental suffering and lockdown. A total of 651 respondents from Rajshahi district in Bangladesh participated in the survey, and by a self-administered questionnaire, designed in the language of English. Among the collected responses, 535 were considered for the analysis, which largely belonged to Rajshahi’s middle to upper socio-economic status (SES). The data analysis was performed using Microsoft Excel 2019 and SPSS version 25.0 (Chicago, IL, USA). The data were analyzed by using descriptive statistic, chi-square test and z-proportional test. In this empirical study, 278 males and 257 females participated. More than one-tenth of males and 35% of females were not conscious of the type of Coronavirus. A lot of misperception about the incubation period of COVID-19 existed among participants. The research observed that 34.2%, of males and 25.3% of the female study population had no opinion about the treatment of COVID-19 and 27.3% of males and 21.8% of females gave a verdict that COVID-19 had no available treatment. The study discovered more surprisingly that females (67.3%), greater than males (65.5%) did not want to stay at home during the lockdown. Positively 87.8% of males and 70.4% of females washed their hands with soap or used sanitizer after coming back from outside. The consumption of a balanced diet increased by males and females was 13.3% and 6.7% respectively. Z-proportional test showed that regarding COVID-19, there were significant difference between male and female’s conception and precaution (p<0.0001). The study has used the first-time data based on the population’s lifestyle and eating habits during the COVID-19 lockdown, which will give assistance to the policy makers to develop the situation.\u0000J Bio-Sci. 30(1): 33-48, 2022 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44944120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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