CC Nganwuchu, K. Habas, N. Mohammed, M. O. Wusuansa, D. Makanjuola, K. Assi, RC Gopalan, M. Nasim
Since December 2019, a new type of coronavirus called novel coronavirus (2019-nCoV, or COVID-19) was identified in Wuhan, China and on March 11, 2020, the World Health Organization (WHO) has declared the novel coronavirus (COVID-19) outbreak a global pandemic. With more than 101,797,158 confirmed cases, resulting in 3,451,354 deaths as of May 21, 2021, the world faces an unprecedented economic, social, and health impact. The clinical spectrum of COVID-19 has a wide range of manifestations, ranging from an asymptomatic state or mild respiratory symptoms to severe viral pneumonia and acute respiratory distress syndrome. Several diagnostic methods are currently available for detecting the coronavirus in clinical, research, and public health laboratories. Some tests detect the infection directly by detecting the viral RNA using real time reverse transcriptase polymerase chain reaction (RT-PCR) and other tests detect the infection indirectly by detecting the host antibodies. Additional techniques are using medical imaging diagnostic tools such as X-ray and computed tomography (CT). Various approaches have been employed in the development of COVID-19 therapies. Some of these approaches use drug repurposing (e.g. Remdesivir and Dexamethasone) and combinational therapy (e.g. Lopinavir/Ritonavir), whilst others aim to develop anti-viral vaccines (e.g. mRNA and antibody). Additionally, health experts integrate data sharing, provide with guidelines and advice to minimize the effects of the pandemic. These guidelines include wearing masks, avoiding direct contact with infectious people, respiratory and personal hygiene. Taken together, an extensive knowledge on transmission mechanisms, clinical spectrums, specific diagnostics methods, host-virus interactions are required to tackle this pandemic. J. Bio-Sci. 29(2): 175-186, 2021 (December)
{"title":"Tackling the COVID-19 Pandemic- A Review","authors":"CC Nganwuchu, K. Habas, N. Mohammed, M. O. Wusuansa, D. Makanjuola, K. Assi, RC Gopalan, M. Nasim","doi":"10.3329/jbs.v29i2.54964","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54964","url":null,"abstract":"Since December 2019, a new type of coronavirus called novel coronavirus (2019-nCoV, or COVID-19) was identified in Wuhan, China and on March 11, 2020, the World Health Organization (WHO) has declared the novel coronavirus (COVID-19) outbreak a global pandemic. With more than 101,797,158 confirmed cases, resulting in 3,451,354 deaths as of May 21, 2021, the world faces an unprecedented economic, social, and health impact. The clinical spectrum of COVID-19 has a wide range of manifestations, ranging from an asymptomatic state or mild respiratory symptoms to severe viral pneumonia and acute respiratory distress syndrome. Several diagnostic methods are currently available for detecting the coronavirus in clinical, research, and public health laboratories. Some tests detect the infection directly by detecting the viral RNA using real time reverse transcriptase polymerase chain reaction (RT-PCR) and other tests detect the infection indirectly by detecting the host antibodies. Additional techniques are using medical imaging diagnostic tools such as X-ray and computed tomography (CT). Various approaches have been employed in the development of COVID-19 therapies. Some of these approaches use drug repurposing (e.g. Remdesivir and Dexamethasone) and combinational therapy (e.g. Lopinavir/Ritonavir), whilst others aim to develop anti-viral vaccines (e.g. mRNA and antibody). Additionally, health experts integrate data sharing, provide with guidelines and advice to minimize the effects of the pandemic. These guidelines include wearing masks, avoiding direct contact with infectious people, respiratory and personal hygiene. Taken together, an extensive knowledge on transmission mechanisms, clinical spectrums, specific diagnostics methods, host-virus interactions are required to tackle this pandemic.\u0000J. Bio-Sci. 29(2): 175-186, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46367872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Sultana, M. Rabbi, B. Sarker, MS Islam, M. Begum, K. Hossain
Milk contains many nutrients including carbohydrate, protein, fat, vitamins and minerals. Milk protein has high nutritional value because it contains all the essential amino acids. Considering the nutritive value, milk is widely consumed by the people as an ideal food. The biochemical changes in milk and milk products by microorganisms can be either desirable or undesirable. The safety of milk and milk products with respect to food borne diseases is of great concern around the world. Therefore, the present study was undertaken to determine the prevalence of Escherichia coli in milk and milk product with their antibiogram assay. A total of 150 milk and milk product (yogurt) samples were collected from Rajshahi Metropolitan area of Bangladesh and analyzed by cultural, staining and biochemical tests for the isolation and identification of E. coli. Antibiogram assay of all the isolates were done by disk diffusion method. The overall prevalence of E. coli was 20.0% in milk and milk product (yogurt) in Rajshahi Metropolitan area. The prevalence of E. coli was 26.0% and 34.0% in raw milk and in milk product (yogurt), respectively. E. coli was not detected in pasteurized milk in this study. In antibiogram assay, isolated E. coli showed 100.0%, 60.0%, 40.0%, 40.0%, 33.3%, 33.3%, 20.0%, and 10.0% resistance to penicillin, gentamycin, ampicillin, streptomycin, amoxycillin, sulfamethoxasole-trimethoprim, nalidixic acid, and ciprofloxacin, respectively. The isolates also showed 73.3%, 60.0%, 53.3%, 53.3%, 30.0%, 23.3%, and 20% sensitive to ciprofloxacin, nalidixic acid, sulfamethoxasole-trimethoprim, streptomycin, amoxycillin, ampicillin, and gentamycin, respectively. The findings of this experiment speculated that the use of ciprofloxacin and nalidixic acid may have the preference in the clinical control of milk borne E. coli infection in Bangladesh. J. Bio-Sci. 29(2): 81-91, 2021 (December)
{"title":"Prevalence and Antibiotic Resistance Patters of Escherichia Coli Isolated From Milk and Milk Product","authors":"T. Sultana, M. Rabbi, B. Sarker, MS Islam, M. Begum, K. Hossain","doi":"10.3329/jbs.v29i2.54957","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54957","url":null,"abstract":"Milk contains many nutrients including carbohydrate, protein, fat, vitamins and minerals. Milk protein has high nutritional value because it contains all the essential amino acids. Considering the nutritive value, milk is widely consumed by the people as an ideal food. The biochemical changes in milk and milk products by microorganisms can be either desirable or undesirable. The safety of milk and milk products with respect to food borne diseases is of great concern around the world. Therefore, the present study was undertaken to determine the prevalence of Escherichia coli in milk and milk product with their antibiogram assay. A total of 150 milk and milk product (yogurt) samples were collected from Rajshahi Metropolitan area of Bangladesh and analyzed by cultural, staining and biochemical tests for the isolation and identification of E. coli. Antibiogram assay of all the isolates were done by disk diffusion method. The overall prevalence of E. coli was 20.0% in milk and milk product (yogurt) in Rajshahi Metropolitan area. The prevalence of E. coli was 26.0% and 34.0% in raw milk and in milk product (yogurt), respectively. E. coli was not detected in pasteurized milk in this study. In antibiogram assay, isolated E. coli showed 100.0%, 60.0%, 40.0%, 40.0%, 33.3%, 33.3%, 20.0%, and 10.0% resistance to penicillin, gentamycin, ampicillin, streptomycin, amoxycillin, sulfamethoxasole-trimethoprim, nalidixic acid, and ciprofloxacin, respectively. The isolates also showed 73.3%, 60.0%, 53.3%, 53.3%, 30.0%, 23.3%, and 20% sensitive to ciprofloxacin, nalidixic acid, sulfamethoxasole-trimethoprim, streptomycin, amoxycillin, ampicillin, and gentamycin, respectively. The findings of this experiment speculated that the use of ciprofloxacin and nalidixic acid may have the preference in the clinical control of milk borne E. coli infection in Bangladesh. J. Bio-Sci. 29(2): 81-91, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Rahman, M. Hossain, Md. Mizanur Rahman, M. Islam, M. A. Rahman, Most. Farida Parvin, Sarmin, B. Sarker, W. Sabbir, Khadija Habib
The long whiskers catfish, Mystus gulio (Hamilton, 1822), is a valuable, delicious food fish in coastal areas of Bangladesh. It is found in fresh and brackish water of South Asian region. The present study illustrates the temporal variation of condition factor (KF) and prey-predator through relative weight (WR) along with length-weight relation (LWR) of M. gulio in the Maloncho River at southern Bangladesh. There were a total of 1200 individuals collected between January-December 2017 and October 2019 to June 2020. Different traditional gears (such as cast net, seine net, square lift net) were used for collecting the fish samples. There were 35% males and 65% females. The total length of fish ranged 7.8-18.3 cm for males and 9.3-22.1 cm for females. The body weight ranged 8.80–72.34 g for males and 8.09-128.82 g for females. LWR was significant with r2 value >0.950. The calculated co-efficient (b) were negative allometric growth in male and female (b<3.0, p<0.001). The KF indicated the best condition of this species. WR revealed no significant difference from 100 for both sex (p = 0.441) and females (p = 0.221), indicating a suitable habitat for growth. From conservation point of view, the findings of this present study on M. gulio will be helpful in Bangladesh (Maloncho River) and neighboring countries. J. Bio-Sci. 29(1): 111-122, 2021 (June)
{"title":"Temporal Variations of Condition Factor and Relative Weight For Mystus Gulio (Hamilton, 1822) From the Coastal Water in Bangladesh","authors":"O. Rahman, M. Hossain, Md. Mizanur Rahman, M. Islam, M. A. Rahman, Most. Farida Parvin, Sarmin, B. Sarker, W. Sabbir, Khadija Habib","doi":"10.3329/jbs.v29i0.54827","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54827","url":null,"abstract":"The long whiskers catfish, Mystus gulio (Hamilton, 1822), is a valuable, delicious food fish in coastal areas of Bangladesh. It is found in fresh and brackish water of South Asian region. The present study illustrates the temporal variation of condition factor (KF) and prey-predator through relative weight (WR) along with length-weight relation (LWR) of M. gulio in the Maloncho River at southern Bangladesh. There were a total of 1200 individuals collected between January-December 2017 and October 2019 to June 2020. Different traditional gears (such as cast net, seine net, square lift net) were used for collecting the fish samples. There were 35% males and 65% females. The total length of fish ranged 7.8-18.3 cm for males and 9.3-22.1 cm for females. The body weight ranged 8.80–72.34 g for males and 8.09-128.82 g for females. LWR was significant with r2 value >0.950. The calculated co-efficient (b) were negative allometric growth in male and female (b<3.0, p<0.001). The KF indicated the best condition of this species. WR revealed no significant difference from 100 for both sex (p = 0.441) and females (p = 0.221), indicating a suitable habitat for growth. From conservation point of view, the findings of this present study on M. gulio will be helpful in Bangladesh (Maloncho River) and neighboring countries.\u0000J. Bio-Sci. 29(1): 111-122, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44837961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. B. M. Zahidul Hoque, M. Alam, S. Khatun, M. Salahin
An experiment was conducted to study the effect of micronutrients on the growth and yield performance of chickpea. The experiment was consisted using two factors- variety and treatments. Two local cultivars viz. BARI Chola-5 and BARI Chola-9 and five treatments e.g. T0 = control, T1 = 2 kg B/ha, T2 = 2 kg Mo/ha, T3= seed priming with Mo (1g/l water), T4 = foliar spray of B (0.5 g/l water) + seed priming with Mo (1g/l water), and T5 = 2 kg B/ha + seed priming with Mo (1g/l water) were considered for this study. A randomized complete block design (RCBD) was adopted with three replications. Significant variation was observed between the varieties and here the BARI Chola-9 produced the higher seed yield (2.47 ton/ ha). Treatment showed significant effect on the yield attributes and yield of chickpea. The highest plant height, number of branches/ plant, number of pods/ plant, nodule/ plant, number of effective pod/ plant, length of the pod, 1000-grain weight, seed yield, straw yield, biological yield was obtained from T4. The highest seed yield (2.68 ton/ ha) was recorded from BARI Chola-9 with foliar spray of boron and seed priming with Mo. The results indicated that foliar spray of B and seed priming with molybdenum can be beneficial in improving growth and yield of chickpea variety BARI Chola-9. J. Bio-Sci. 29(2): 43-51, 2021 (December)
通过试验研究了微量营养元素对鹰嘴豆生长和产量的影响。试验采用品种和处理两种因素。本研究考虑了2个地方品种BARI Chola-5和BARI Chola-9，以及T0 =对照，T1 = 2 kg B/ha, T2 = 2 kg Mo/ha, T3= Mo (1g/l水)引种，T4 =叶片喷施B (0.5 g/l水)+ Mo (1g/l水)引种，T5 = 2 kg B/ha + Mo (1g/l水)引种。采用随机完全区组设计(RCBD)，共3个重复。品种间存在显著差异，其中BARI Chola-9的种子产量最高(2.47吨/公顷)。处理对鹰嘴豆的产量性状和产量有显著影响。通过T4获得最高株高、枝数/株、荚果数/株、根瘤数/株、有效荚果数/株、荚果长、千粒重、种子产量、秸秆产量、生物产量。叶面喷硼+ Mo种子催收的BARI Chola-9种子产量最高，为2.68 t / hm2。结果表明，叶面喷硼+ Mo种子催收有利于提高BARI Chola-9 - j的生长和产量。Bio-Sci。29(2): 43-51, 2021(12月)
{"title":"Response of Chickpea (Cicer Arietinum L.) To Boron and Molybdenum Fertilization","authors":"A. B. M. Zahidul Hoque, M. Alam, S. Khatun, M. Salahin","doi":"10.3329/jbs.v29i2.54953","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54953","url":null,"abstract":"An experiment was conducted to study the effect of micronutrients on the growth and yield performance of chickpea. The experiment was consisted using two factors- variety and treatments. Two local cultivars viz. BARI Chola-5 and BARI Chola-9 and five treatments e.g. T0 = control, T1 = 2 kg B/ha, T2 = 2 kg Mo/ha, T3= seed priming with Mo (1g/l water), T4 = foliar spray of B (0.5 g/l water) + seed priming with Mo (1g/l water), and T5 = 2 kg B/ha + seed priming with Mo (1g/l water) were considered for this study. A randomized complete block design (RCBD) was adopted with three replications. Significant variation was observed between the varieties and here the BARI Chola-9 produced the higher seed yield (2.47 ton/ ha). Treatment showed significant effect on the yield attributes and yield of chickpea. The highest plant height, number of branches/ plant, number of pods/ plant, nodule/ plant, number of effective pod/ plant, length of the pod, 1000-grain weight, seed yield, straw yield, biological yield was obtained from T4. The highest seed yield (2.68 ton/ ha) was recorded from BARI Chola-9 with foliar spray of boron and seed priming with Mo. The results indicated that foliar spray of B and seed priming with molybdenum can be beneficial in improving growth and yield of chickpea variety BARI Chola-9. J. Bio-Sci. 29(2): 43-51, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
KS Sultana, PS Brishti, S. Ahmed, M. Billah, K. Habib
The present study reports the morphological and molecular characterization of marine neogastropods collected from the South-East to the South-West coasts of Bangladesh. A total of 21 species under 13 families were identified morphologically, of which 7 species were barcoded successfully using partial sequence of mitochondrial cytochrome oxidase subunit-I (COI) gene. The average nucleotide frequencies of these species were G (guanine) = 20.57%, C (cytosine) = 18.44%, A (adenine) = 23.65%, T (thymine) = 37.35% and the GC content was 39.01%. The average Kimura two-parameter (K2P) distances of the COI barcode sequences within species, genera, and families were 20.7%, 22.0%, and 22.0%, respectively. The average inter-species genetic divergence was calculated as 21.0%. In addition, the COI barcode sequence of Nassarius stolatus was identified and submitted to the GenBank for the first time. The study also reports the new record of a species, Indothais rufotincta Tan & Sigurdsson, 1996 from Bangladesh. This finding greatly extends the distributional range of this species from the West coast to the East coast of the Bay of Bengal. J. Bio-Sci. 29(1): 79-91, 2021 (June)
本研究报告了从孟加拉国东南至西南海岸收集的海洋新胃足类动物的形态和分子特征。形态学鉴定13科21种，利用线粒体细胞色素氧化酶亚单位- i (COI)基因部分序列成功对其中7种进行了条形码鉴定。这些物种的平均核苷酸频率分别为G(鸟嘌呤)= 20.57%、C(胞嘧啶)= 18.44%、A(腺嘌呤)= 23.65%、T(胸腺嘧啶)= 37.35%，GC含量为39.01%。COI条形码序列在种、属和科内的平均木村双参数(K2P)距离分别为20.7%、22.0%和22.0%。种间遗传差异平均值为21.0%。此外，还首次鉴定出了白刺柳COI条形码序列，并提交给GenBank。该研究还报告了一个物种的新记录，1996年来自孟加拉国的indothai rufotinta Tan & Sigurdsson。这一发现大大扩大了该物种的分布范围，从西海岸到东海岸的孟加拉湾。Bio-Sci。29(1): 79-91, 2021(6月)
{"title":"Morphological and Molecular Characterization of Several Neogastropod Species (Mollusca: Gastropoda) From Coastal Waters of Bangladesh With One New Record","authors":"KS Sultana, PS Brishti, S. Ahmed, M. Billah, K. Habib","doi":"10.3329/jbs.v29i0.54824","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54824","url":null,"abstract":"The present study reports the morphological and molecular characterization of marine neogastropods collected from the South-East to the South-West coasts of Bangladesh. A total of 21 species under 13 families were identified morphologically, of which 7 species were barcoded successfully using partial sequence of mitochondrial cytochrome oxidase subunit-I (COI) gene. The average nucleotide frequencies of these species were G (guanine) = 20.57%, C (cytosine) = 18.44%, A (adenine) = 23.65%, T (thymine) = 37.35% and the GC content was 39.01%. The average Kimura two-parameter (K2P) distances of the COI barcode sequences within species, genera, and families were 20.7%, 22.0%, and 22.0%, respectively. The average inter-species genetic divergence was calculated as 21.0%. In addition, the COI barcode sequence of Nassarius stolatus was identified and submitted to the GenBank for the first time. The study also reports the new record of a species, Indothais rufotincta Tan & Sigurdsson, 1996 from Bangladesh. This finding greatly extends the distributional range of this species from the West coast to the East coast of the Bay of Bengal. J. Bio-Sci. 29(1): 79-91, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Hossain, W. Rahman, M.S. Ali, T. Sultana, K. Hossain
Chicken eggs contain all the essential components such as proteins, lipids, vitamins, minerals, carbohydrates, and growth factors required by the human being. Despite of their nutritional values eggs can cause health problems through consumption of contaminated eggs with pathogenic microorganisms. Therefore, the present study was undertaken to identify Escherichia coli isolated from chicken eggs with their antibiogram assay in Rajshahi district of Bangladesh. E. coli was isolated from 180 chicken eggs collected from different areas of Rajshahi district and identified based on cultural, staining, and biochemical characteristics. Antibiogram assay of all the isolates were done by disk diffusion method. The overall prevalence of E. coli was 38.89% in chicken eggs of which 27.78% was on egg shells and 11.11% was in egg content. The prevalence of E. coli was 58.33% in commercial layer farm eggs, 41.66% in whole seller eggs, and 16.67% in retailer eggs. In antibiogram study, isolated E. coli showed 64.28% to 92.85% sensitivity to chloramphenicol, gentamycin, and ceftriaxone. The highest sensitivity was found to meropenem (100%). Isolated E. coli showed resistance to ciprofloxacin, tetracycline, amoxicillin, ampicillin, and erythromycin ranging from 50% to 71.42%. Judicious use of antibiotics and public awareness will help to reduce the development of antibiotic resistance. J. Bio-Sci. 29(1): 123-133, 2021 (June)
{"title":"Identification and Antibiogram Assay of Escherichia Coli Isolated From Chicken Eggs","authors":"M. Hossain, W. Rahman, M.S. Ali, T. Sultana, K. Hossain","doi":"10.3329/jbs.v29i0.54828","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54828","url":null,"abstract":"Chicken eggs contain all the essential components such as proteins, lipids, vitamins, minerals, carbohydrates, and growth factors required by the human being. Despite of their nutritional values eggs can cause health problems through consumption of contaminated eggs with pathogenic microorganisms. Therefore, the present study was undertaken to identify Escherichia coli isolated from chicken eggs with their antibiogram assay in Rajshahi district of Bangladesh. E. coli was isolated from 180 chicken eggs collected from different areas of Rajshahi district and identified based on cultural, staining, and biochemical characteristics. Antibiogram assay of all the isolates were done by disk diffusion method. The overall prevalence of E. coli was 38.89% in chicken eggs of which 27.78% was on egg shells and 11.11% was in egg content. The prevalence of E. coli was 58.33% in commercial layer farm eggs, 41.66% in whole seller eggs, and 16.67% in retailer eggs. In antibiogram study, isolated E. coli showed 64.28% to 92.85% sensitivity to chloramphenicol, gentamycin, and ceftriaxone. The highest sensitivity was found to meropenem (100%). Isolated E. coli showed resistance to ciprofloxacin, tetracycline, amoxicillin, ampicillin, and erythromycin ranging from 50% to 71.42%. Judicious use of antibiotics and public awareness will help to reduce the development of antibiotic resistance. J. Bio-Sci. 29(1): 123-133, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proper germination and proper growth of seedlings in conventional farming methods are often not achieved at the desired rate. The purpose of priming is to reduce germination time and improve stand and percentage of germination and increases vigour for fast and strong plant development. In order to evaluate the effect of seed priming on growth and yield of wheat the present study was conducted at Agronomy Farm House, Rajshahi University. Three wheat varieties (BARI Gom-24, BARI Gom-25 and BARI Gom-26) were tested with three groups of priming media (osmo-priming, thermos-priming and plant growth regulator priming) and control (non-soaked). Seeds were primed for 24 h at 25°C with four priming media, osmo-priming (1% NaCl, 2% KNO3, 2% KH2PO4, and 10% PEG), PEG-Priming (50 μM GA3 and 50 μM BAP) and thermo-priming (0°C, 5°C and 10°C) and control was without priming agent. All types of priming media were significantly different among their types for plant height (cm), tiller number and dry biomass (g). PEG was significantly different in osmo-priming, GA3 was significantly different in PGR-priming and 0°C was significantly different in thermo-priming. In case of vigor index, 2% KH2PO4 was suitable among osmo-priming, GA3 was better than BAP priming and 0°C was optimum among all temperature priming. Seed priming helps seeds to grow rapidly and evenly in the field, producing healthier and faster maturing crops with better yields. J. Bio-Sci. 29(2): 111-122, 2021 (December)
{"title":"Effects of Osmotic, Thermal and Plant Growth Regulators Seed Priming on Different Wheat Varieties","authors":"M. Ahmed, F. Khatun, Z. Islam","doi":"10.3329/jbs.v29i2.54960","DOIUrl":"https://doi.org/10.3329/jbs.v29i2.54960","url":null,"abstract":"Proper germination and proper growth of seedlings in conventional farming methods are often not achieved at the desired rate. The purpose of priming is to reduce germination time and improve stand and percentage of germination and increases vigour for fast and strong plant development. In order to evaluate the effect of seed priming on growth and yield of wheat the present study was conducted at Agronomy Farm House, Rajshahi University. Three wheat varieties (BARI Gom-24, BARI Gom-25 and BARI Gom-26) were tested with three groups of priming media (osmo-priming, thermos-priming and plant growth regulator priming) and control (non-soaked). Seeds were primed for 24 h at 25°C with four priming media, osmo-priming (1% NaCl, 2% KNO3, 2% KH2PO4, and 10% PEG), PEG-Priming (50 μM GA3 and 50 μM BAP) and thermo-priming (0°C, 5°C and 10°C) and control was without priming agent. All types of priming media were significantly different among their types for plant height (cm), tiller number and dry biomass (g). PEG was significantly different in osmo-priming, GA3 was significantly different in PGR-priming and 0°C was significantly different in thermo-priming. In case of vigor index, 2% KH2PO4 was suitable among osmo-priming, GA3 was better than BAP priming and 0°C was optimum among all temperature priming. Seed priming helps seeds to grow rapidly and evenly in the field, producing healthier and faster maturing crops with better yields. J. Bio-Sci. 29(2): 111-122, 2021 (December)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69504129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meiotic behavior of ten pteridophyte species from Bangladesh was analyzed in the present investigation. In SMCs of Adiantum capillus-veneris, thirty distinct bivalents were enumerated. In A. caudatum 16 SMCs resulting 64 spores were observed in sporangium which appeared to be diploid sexual. A. lunulatum was found to be triploid apogamous and as diploid sexual forms, thus this species of Adiantum exhibited wide range of morphological variations. Pteris biaurita, P. graffithi and P. vittata were recorded to be n = 29 as diploid. In thelypterids, all the four species were observed to be diploid sexual except C. dentata when it was tetraploid (n = 36, 4x = 144). In C. arida, meiosis was almost regular with 2n = 72 chromosomes. C. cylindrothrix and Ampelopteris prolifera were also found to be diploid sexual, with 2n = 72 chromosomes. J. Bio-Sci. 29(1): 135-142, 2021 (June)
{"title":"Meiotic Behaviour in Ten Species of Pteridophytes From Bangladesh","authors":"M. Mannan, G. Kabir","doi":"10.3329/jbs.v29i0.54829","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54829","url":null,"abstract":"Meiotic behavior of ten pteridophyte species from Bangladesh was analyzed in the present investigation. In SMCs of Adiantum capillus-veneris, thirty distinct bivalents were enumerated. In A. caudatum 16 SMCs resulting 64 spores were observed in sporangium which appeared to be diploid sexual. A. lunulatum was found to be triploid apogamous and as diploid sexual forms, thus this species of Adiantum exhibited wide range of morphological variations. Pteris biaurita, P. graffithi and P. vittata were recorded to be n = 29 as diploid. In thelypterids, all the four species were observed to be diploid sexual except C. dentata when it was tetraploid (n = 36, 4x = 144). In C. arida, meiosis was almost regular with 2n = 72 chromosomes. C. cylindrothrix and Ampelopteris prolifera were also found to be diploid sexual, with 2n = 72 chromosomes. J. Bio-Sci. 29(1): 135-142, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Acalypha indica L. is an herbaceous medicinal plant, which is grown abundantly in Bangladesh. Bangladeshi people typically use various parts of this plant for various primary healthcare treatments. The current research was conducted to check and quantify various phytochemicals and mineral components of the assorted parts of Acalypha indica L. The screening analysis shows that studied all phytochemicals were present in the leaf part but not in stem and shoot parts and some were missing or trace amount in root. Total phenol, flavonoid, and tannins content on a dry weight basis ranged from 0.53-0.82 mg GAE/g, 0.02-1.47 mg QE/g and 0.21-0.32 mg TAE/g respectively. Among the phytochemicals the leaf contains the highest amount of flavonoid (1.47 mg QE/g), and lowest in stem (0.02 mg QE/g). Similarly, the amount of minerals like Fe, Zn, K and Pb was 0.23-0.40 mg/kg, 0.01- 0.021 mg/kg, 1.55-2.85 mg/kg and 0.149-0.204 mg/kg respectively. K was the largest volume of mineral for both leaf and stem. Lowest quantity of Zn was observed in most cases. This finding indicates that the studied plant might be good source of phytochemicals. J. Bio-Sci. 29(1): 69-77, 2021 (June)
{"title":"Phytochemical Analysis of Different Parts of Acalypha Indica L.","authors":"Mim E Tasmim, M. Nasiruddin, M. Islam, RS Sultana","doi":"10.3329/jbs.v29i0.54823","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54823","url":null,"abstract":"Acalypha indica L. is an herbaceous medicinal plant, which is grown abundantly in Bangladesh. Bangladeshi people typically use various parts of this plant for various primary healthcare treatments. The current research was conducted to check and quantify various phytochemicals and mineral components of the assorted parts of Acalypha indica L. The screening analysis shows that studied all phytochemicals were present in the leaf part but not in stem and shoot parts and some were missing or trace amount in root. Total phenol, flavonoid, and tannins content on a dry weight basis ranged from 0.53-0.82 mg GAE/g, 0.02-1.47 mg QE/g and 0.21-0.32 mg TAE/g respectively. Among the phytochemicals the leaf contains the highest amount of flavonoid (1.47 mg QE/g), and lowest in stem (0.02 mg QE/g). Similarly, the amount of minerals like Fe, Zn, K and Pb was 0.23-0.40 mg/kg, 0.01- 0.021 mg/kg, 1.55-2.85 mg/kg and 0.149-0.204 mg/kg respectively. K was the largest volume of mineral for both leaf and stem. Lowest quantity of Zn was observed in most cases. This finding indicates that the studied plant might be good source of phytochemicals. J. Bio-Sci. 29(1): 69-77, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetic peripheral neuropathy (DPN) is one of the most common long-term microvascular complications of diabetes. This study was undertaken to investigate the association of HbA1c variability with diabetic peripheral neuropathy (sensory and motor) in patients with type-2 diabetes. In this cross-sectional study, a total of 150 type-2 diabetic patients were screened for DPN and undergone quarterly HbA1c measurements during the year preceding enrolment were recruited. DPN was confirmed in patients displaying both clinical manifestations of neuropathy and neurological abnormalities assessment. Among the recruited patients, 24% (n = 36) were found to have DPN, and these patients also presented with a higher HbA1c as compared to the patients without DPN (p<0.05). In addition, the advanced age and longer duration of diabetes were important and significant (p<0.05) risk factors for peripheral neuropathy. Overall study suggests that increased HbA1c level is strongly associated with DPN (sensory and motor) in type-2 diabetic patients and could be considered as a potent indicator for DPN in the recruited patients. J. Bio-Sci. 29(1): 93-100, 2021 (June)
{"title":"Hba1c Variability Has a Strong Relationship With Peripheral Sensory And Motor Neuropathy in Type-2 Diabetes Mellitus","authors":"Mahbub Hossain, Mk Sarkar, I. Mahbub, S. Islam","doi":"10.3329/jbs.v29i0.54825","DOIUrl":"https://doi.org/10.3329/jbs.v29i0.54825","url":null,"abstract":"Diabetic peripheral neuropathy (DPN) is one of the most common long-term microvascular complications of diabetes. This study was undertaken to investigate the association of HbA1c variability with diabetic peripheral neuropathy (sensory and motor) in patients with type-2 diabetes. In this cross-sectional study, a total of 150 type-2 diabetic patients were screened for DPN and undergone quarterly HbA1c measurements during the year preceding enrolment were recruited. DPN was confirmed in patients displaying both clinical manifestations of neuropathy and neurological abnormalities assessment. Among the recruited patients, 24% (n = 36) were found to have DPN, and these patients also presented with a higher HbA1c as compared to the patients without DPN (p<0.05). In addition, the advanced age and longer duration of diabetes were important and significant (p<0.05) risk factors for peripheral neuropathy. Overall study suggests that increased HbA1c level is strongly associated with DPN (sensory and motor) in type-2 diabetic patients and could be considered as a potent indicator for DPN in the recruited patients. J. Bio-Sci. 29(1): 93-100, 2021 (June)","PeriodicalId":90580,"journal":{"name":"Journal of bio-science","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69503903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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