Hiroyuki Kikuchi, M. Yoshizawa, K. Tanemura, E. Sato, Hiroaki Yoshida
Abstract: Recently, the quality of in vitro matured (IVM) oocytes has been improved by numerous studies using animal models. However, the implantation rates of IVM oocytes remain lower than those of in vitro fertilization (IVF). Chromosome abnormalities, which mainly occur during the first meiotic division, are often observed in IVM oocytes. To clarify how the properties of the cumulus cell layers relate to chromosome abnormalities of oocytes during IVM, we investigated the occurrence of aneuploidy and developmental competence using two different media in a mouse model. Maturation rates were significantly lower in denuded oocytes at oocyte collection in both types of maturation media as compared with those of oocytes mechanically denuded just after collection of cumulus-oocyte complex. Fertilization rates and blastocyst rates were lower in Waymouth's MB752/1 medium than in HTF medium. The occurrence of premature chromatid separation (PCS) remarkably increased in Waymouth's MB752/1 medium compared to HTF medium. This demonstrates that denuded oocytes at oocyte collection have low ability to progress to nuclear maturation under IVM conditions. However, the fertilization ability and developmental competence to the blastocyst stage of denuded oocytes were fulfilled under a suitable culture condition. We suggest that oocytes that are already denuded, before or during oocyte collection, have the potential to be matured in vitro if the denuded oocytes are cultured in a suitable culture media.
{"title":"Risk of Premature Chromatid Separation is Increased by Poor Cumulus Cell Layers and Inappropriate Culture Media for in vitro Maturation of Mouse Oocytes","authors":"Hiroyuki Kikuchi, M. Yoshizawa, K. Tanemura, E. Sato, Hiroaki Yoshida","doi":"10.1274/jmor.33.63","DOIUrl":"https://doi.org/10.1274/jmor.33.63","url":null,"abstract":"Abstract: \u0000 Recently, the quality of in vitro matured (IVM) oocytes has been improved by numerous studies using animal models. However, the implantation rates of IVM oocytes remain lower than those of in vitro fertilization (IVF). Chromosome abnormalities, which mainly occur during the first meiotic division, are often observed in IVM oocytes. To clarify how the properties of the cumulus cell layers relate to chromosome abnormalities of oocytes during IVM, we investigated the occurrence of aneuploidy and developmental competence using two different media in a mouse model. Maturation rates were significantly lower in denuded oocytes at oocyte collection in both types of maturation media as compared with those of oocytes mechanically denuded just after collection of cumulus-oocyte complex. Fertilization rates and blastocyst rates were lower in Waymouth's MB752/1 medium than in HTF medium. The occurrence of premature chromatid separation (PCS) remarkably increased in Waymouth's MB752/1 medium compared to HTF medium. This demonstrates that denuded oocytes at oocyte collection have low ability to progress to nuclear maturation under IVM conditions. However, the fertilization ability and developmental competence to the blastocyst stage of denuded oocytes were fulfilled under a suitable culture condition. We suggest that oocytes that are already denuded, before or during oocyte collection, have the potential to be matured in vitro if the denuded oocytes are cultured in a suitable culture media.","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"13 1","pages":"63 - 68"},"PeriodicalIF":0.0,"publicationDate":"2016-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84912082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preface: Culture Media for Preimplantation Development","authors":"T. Kasai","doi":"10.1274/032.033.0102","DOIUrl":"https://doi.org/10.1274/032.033.0102","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"48 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2016-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76295092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preface: Cellular Remodeling and Epigenetic Reprogramming during the Development of Germ Line Cells","authors":"Kazuya Matsumoto","doi":"10.1274/JMOR.30.67","DOIUrl":"https://doi.org/10.1274/JMOR.30.67","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"2 1","pages":"67-67"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76313450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differentiation in Early Development of Embryo to Placental Formation in Mammalian Species : The Roles of Specific Gene Regulation and Expression in Placental Formation","authors":"Y. Kawano","doi":"10.1274/JMOR.30.1","DOIUrl":"https://doi.org/10.1274/JMOR.30.1","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"33 1","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76736539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Parthenogenesis is the process by which an oocyte develops into an embryo without fertilization. Parthenogenetic activation can be performed at various stages of meiosis, yielding embryos with a distinct genetic pattern of homozygousity and heterozygousity. The heterozygousity pattern specific to parthenogenetic embryonic stem (pES) cells derived from such embryos, can be predicted using genome-wide single nucleotide polymorphism (SNP) analysis to determine whether extrusion of the first or second polar body is prohibited. The heterozygous pES cells carrying the full complement of major histocompatibility complex (MHC) antigen matched to the oocyte donor, could therefore provide a potential source of MHC matched cells or tissue for cell replacement therapy. In this review, we summarized the mechanism of deriving heterozygous MHC-matched pES cells using a mouse and human models.
{"title":"Histocompatible parthenogenetic embryonic stem cells as a potential source for regenerative medicine.","authors":"Akiko Yabuuchi, Haniya Rehman, Kitai Kim","doi":"10.1274/jmor.29.17","DOIUrl":"https://doi.org/10.1274/jmor.29.17","url":null,"abstract":"<p><p>Parthenogenesis is the process by which an oocyte develops into an embryo without fertilization. Parthenogenetic activation can be performed at various stages of meiosis, yielding embryos with a distinct genetic pattern of homozygousity and heterozygousity. The heterozygousity pattern specific to parthenogenetic embryonic stem (pES) cells derived from such embryos, can be predicted using genome-wide single nucleotide polymorphism (SNP) analysis to determine whether extrusion of the first or second polar body is prohibited. The heterozygous pES cells carrying the full complement of major histocompatibility complex (MHC) antigen matched to the oocyte donor, could therefore provide a potential source of MHC matched cells or tissue for cell replacement therapy. In this review, we summarized the mechanism of deriving heterozygous MHC-matched pES cells using a mouse and human models.</p>","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"29 1","pages":"17-21"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1274/jmor.29.17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32703459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"特集：卵子のエネルギー代謝 ―ミトコンドリア機能について―","authors":"直 山海","doi":"10.1274/JMOR.29.145","DOIUrl":"https://doi.org/10.1274/JMOR.29.145","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"312 1","pages":"145"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79705714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Journal of Mammalian Ova Research: Preface","authors":"J. Otsuki","doi":"10.1274/JMOR.26.1","DOIUrl":"https://doi.org/10.1274/JMOR.26.1","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2009-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76407763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"特集：強拡大顕微鏡による形態良好精子の選別～精子形態と受精卵の妊孕性","authors":"直昭 久慈","doi":"10.1274/JMOR.25.231","DOIUrl":"https://doi.org/10.1274/JMOR.25.231","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"223 1","pages":"231-231"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76696784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"特集：選択的単一胚移植（eSET）","authors":"直昭 久慈","doi":"10.1274/JMOR.25.205","DOIUrl":"https://doi.org/10.1274/JMOR.25.205","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"14 1","pages":"205-205"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82510658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hideaki Watanabe, Kaoru Miya, A. Yanaihara, A. Arichi, Kaori Suga, S. Ishikawa, Shiho Monzen, Y. Morimatsu, Yukiko Tazawa, H. Motoyama, Tsuyoshi Yorimitsu, T. Kawamura
{"title":"How to Choice the Culture Media in Our Clinic","authors":"Hideaki Watanabe, Kaoru Miya, A. Yanaihara, A. Arichi, Kaori Suga, S. Ishikawa, Shiho Monzen, Y. Morimatsu, Yukiko Tazawa, H. Motoyama, Tsuyoshi Yorimitsu, T. Kawamura","doi":"10.1274/JMOR.24.177","DOIUrl":"https://doi.org/10.1274/JMOR.24.177","url":null,"abstract":"","PeriodicalId":90599,"journal":{"name":"Journal of mammalian ova research","volume":"9 1","pages":"177-178"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88383801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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