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Comparative study on the metabolic behaviour of anode biofilm in microbial fuel cell under different external resistance 不同外阻条件下微生物燃料电池阳极生物膜代谢行为的比较研究
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2055491
Yana Mersinkova, Hyusein Yemendzhiev, V. Nenov
Abstract In this study the influence of the external circuit resistance on the metabolic behaviour of anodic biofilm in Microbial fuel cell (MFC) was studied. The results obtained demonstrated that extremely low and extremely high circuit loads could deteriorate the bio-electrochemistry of anodic respiration by shifting the microbial metabolism towards typical fermentation of the substrates. The best conditions for respiration and efficient substrate mineralisation of nearly 70% were found in the MFC reactor with 0.1 kΩ resistance in the anode and cathode connecting circuit. Two species of electrochemically active bacteria were isolated from the anodic community and were taxonomically affiliated to Pseudomonas and Bacillus genera based on 16 s rRNA genes amplification and sequencing.
摘要本文研究了外路电阻对微生物燃料电池(MFC)阳极生物膜代谢行为的影响。结果表明,极低和极高的电路负荷会通过将微生物代谢转向底物的典型发酵而使阳极呼吸的生物电化学恶化。阳极和阴极连接电路电阻为0.1 kΩ时,MFC反应器的呼吸作用条件最佳,底物矿化效率接近70%。从阳极菌群中分离到两种具有电化学活性的细菌,经16s rRNA基因扩增和测序,分别归属于假单胞菌和芽孢杆菌属。
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引用次数: 1
Two QTL for kernel number per spike identified from durum wheat 硬粒小麦每穗粒数的两个QTL
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2054728
Jingtian Yang, Zhengqiao Liao, Baoguo Du, K. Zhang, Lei Liu
Abstract As one of the three yield components, kernel number per spike (KNPS) plays a key role in determining wheat yield. In this study, in combination with the previously constructed genetic map for a tetraploid wheat recombinant inbred line (RIL) population developed from the cross between a durum wheat and a wild emmer wheat and phenotype data from four environments, two major and stably expressed quantitative trait loci (QTL) for KNPS were identified. The phenotypic variation explained by these two QTL, Qknps-DW-3A.1 and Qknps-DW-3A.2, was up to 12.52% and 29.52%, respectively. The positive alleles were both from the durum wheat. Pyramiding analysis suggested that the combination of these two positive alleles has the largest effect on increasing KNPS. Comparison of physical interval for these two QTL with those reported previously showed that they may be novel loci. Genetic correlations between KNPS and other agronomic traits were also evaluated. Taken together, the two major and stably expressed QTL for KNPS from tetraploid wheat reported here they should be useful in wheat breeding.
摘要穗粒数(KNPS)作为小麦产量的三个组成部分之一,在决定小麦产量中起着关键作用。在本研究中,结合先前构建的由硬粒小麦和野生二粒小麦杂交形成的四倍体小麦重组自交系(RIL)群体的遗传图谱和来自四种环境的表型数据,鉴定了KNPS的两个主要且稳定表达的数量性状基因座(QTL)。Qknps-DW-3A.1和Qknps-DW-3A.2两个QTL解释的表型变异分别高达12.52%和29.52%。阳性等位基因均来自硬粒小麦。金字塔分析表明,这两个阳性等位基因的组合对KNPS的增加影响最大。将这两个QTL的物理间隔与先前报道的QTL进行比较,表明它们可能是新的基因座。并对KNPS与其它农艺性状的遗传相关性进行了评价。总之,本文报道的两个主要且稳定表达的四倍体小麦KNPS QTL应可用于小麦育种。
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引用次数: 0
An immobilization-based, loop-mediated isothermal amplification device for nucleic acid detection of SARS-CoV-2 N gene 基于固定化环介导的sars - cov - 2n基因核酸检测等温扩增装置
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2134822
Tianyu Song, Chang Chen, Shaoheng Bao, Bin Du, Xiaokun Wang, Jiajia Liu, Fuli Wang, Wei Ma, G. Yao, Xiukun Wan, Xinlong Zhang, JingJing Wang, Hui Jiang
Abstract Confronting the global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), simple, fast and specific non-laboratory SARS-CoV-2 diagnostic tests are urgently required. However, the current nucleic acid assays generally rely on the diagnostic laboratory, trained staff and specialized equipment for execution and analysis, presenting clear limitations in the field detection. Here, we describe a portable and reliable immobilization-based loop-mediated isothermal amplification (LAMP) device which is mobile, without the requirement of any complicated instrument and appropriate for high-throughput testing. This device was constructed by utilizing the interaction between a carboxyl-tagged primer and an amino-tagged substrate, and capable of catching the target sequence in SARS-CoV-2 produced via the immobilization-based LAMP. In this study, the immobilization conditions and immobilized primer structure were explored and optimized. With this proposed device, the analysis result can be obtained rapidly in 30 min with excellent specificity, even if the template is extracted from a complex sample containing pharyngeal swab or human blood. In addition, the device can be applied to detect the nucleic acid of SARS-CoV-2 and various other pathogens, showing attractive potential for rapid and high-throughput detection at airports, railway stations, cold-chain transportations, community hospitals and so on. Therefore, we believe that the immobilization-based LAMP device is an advanced approach to developing a portable, specific, low-cost and high-throughput diagnostic platform.
面对SARS-CoV-2在全球的传播,迫切需要简单、快速、特异性的非实验室诊断方法。然而,目前的核酸检测通常依赖于诊断实验室、训练有素的工作人员和专门的设备来执行和分析,在现场检测方面存在明显的局限性。在这里,我们描述了一种便携式和可靠的基于固定化的环介导等温扩增(LAMP)装置,它是可移动的,不需要任何复杂的仪器,适合于高通量测试。该装置利用羧基标记的引物与氨基标记的底物之间的相互作用构建,能够捕获通过固定化LAMP产生的SARS-CoV-2中的目标序列。本研究对固定化条件和固定化引物结构进行了探索和优化。使用该装置,即使从含有咽拭子或人血的复杂样品中提取模板,也可以在30分钟内快速获得分析结果,具有极好的特异性。此外,该装置可用于检测SARS-CoV-2核酸和其他多种病原体,在机场、火车站、冷链运输、社区医院等领域具有快速、高通量检测的潜力。因此,我们相信基于固定的LAMP设备是开发便携式,特异性,低成本和高通量诊断平台的先进方法。
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引用次数: 0
Autologous ovarian platelet rich plasma treatment improves oocyte and embryo quality: a before-after prospective study 自体卵巢富血小板血浆治疗改善卵母细胞和胚胎质量:一项前后前瞻性研究
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2090280
D. Parvanov, R. Ganeva, N. Vidolova, K. Nikolova, Magdalena Vasileva, T. Totev, G. Stamenov
Abstract Platelet-rich plasma (PRP) is used for successful regeneration of female reproductive tissues. However, little is known about the effect of ovarian PRP treatment on oocyte and embryo quality. The objective of our study was to assess the role of autologous ovarian PRP treatment on ovarian reserve and number and quality of oocytes and embryos in women with poor ovarian response (POR) undergoing in-vitro fertilization cycles. A total of 66 women with POR were treated with ovarian PRP injection in two subsequent menstrual cycles. The antral follicle counts, serum anti-Mullerian hormone, follicle-stimulating hormone levels, fertilization rate, number and quality of oocytes, and embryos were assessed and compared between the cycle before and after PRP treatment. Ovarian PRP treatment resulted in insignificantly lower follicle-stimulating hormone levels, significantly higher antral follicle count, anti-Mullerian hormone, number of retrieved oocytes, and insignificantly higher fertilization rate. However, the mean number of Day 5 embryos (2.19 ± 1.45 vs. 1.58 ± 1.30, p = 0.01), the percentage of high-quality oocytes (45.29% ± 42.40% vs. 15.21% ± 30.24%, p < 0.01) and the percentage of grade-I blastocysts (52.10% ± 37.94% vs. 12.86% ± 22.97%, p < 0.01) were significantly higher after PRP treatment in comparison to the pretreatment period. Moreover, the mean MII oocyte quality (1.60 ± 0.54 vs. 2.31 ± 0.63, p < 0.01) and mean blastocyst quality (1.53 ± 0.45 vs. 2.42 ± 0.63, p < 0.01) were significantly improved in the post-treatment period. In conclusion, the applied autologous ovarian PRP treatment in poor responders may significantly improve oocyte and embryo quality.
富血小板血浆(PRP)用于女性生殖组织的成功再生。然而,目前对卵巢PRP处理对卵母细胞和胚胎质量的影响知之甚少。本研究的目的是评估自体卵巢PRP治疗对体外受精周期卵巢反应差(POR)女性卵巢储备、卵母细胞和胚胎数量和质量的作用。共有66名患有POR的妇女在随后的两个月经周期中接受卵巢PRP注射治疗。评估和比较PRP治疗前后周期的卵泡计数、血清抗苗勒管激素、促卵泡激素水平、受精率、卵母细胞数量和质量、胚胎数量和质量。卵巢PRP治疗导致促卵泡激素水平无显著降低,窦卵泡计数、抗苗勒管激素、卵母细胞数量显著升高,受精率无显著升高。然而,PRP处理后的第5天平均胚胎数(2.19±1.45比1.58±1.30,p = 0.01)、优质卵母细胞百分比(45.29%±42.40%比15.21%±30.24%,p < 0.01)和一级囊胚百分比(52.10%±37.94%比12.86%±22.97%,p < 0.01)均显著高于预处理期。治疗后各组平均MII卵母细胞质量(1.60±0.54比2.31±0.63,p < 0.01)和囊胚质量(1.53±0.45比2.42±0.63,p < 0.01)均显著提高。综上所述,对不良反应者应用自体卵巢PRP治疗可显著改善卵母细胞和胚胎质量。
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引用次数: 2
Optimization of succinic acid production from xylose mother liquor (XML) by Actinobacillus succinogenes using response surface methodology 响应面法优化琥珀酸放线菌木糖母液产琥珀酸的工艺条件
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2095303
N. Shen, Shiyong Li, Yan Qin, Mingguo Jiang, Hongyan Zhang
Abstract In the present study, conditions for succinic acid (SA) production using xylose mother liquor (XML) as culture medium by Actinobacillus succinogenes GXAS137 were optimized. Firstly, single–factor experiments were performed to evaluate the basal culture medium for SA fermentation. Thereafter, the Plackett–Burman design was used to screen out three significant factors of XML, corn steep liquor powder (CSLP) and MgCO3 affecting the SA yields from the original nine factors. Subsequent use of steepest ascent experiment determined the center area of the three factors. Finally, the response surface methodology was used to further optimize the interactions between the three main factors and predict the maximum SA concentration through Box-Behnken design. The optimal conditions of SA fermentation were maximally documented in the XML (110 g/L), CSLP (18.86 g/L) and MgCO3 (69.12 g/L). The maximum production of SA was 58.06 ± 0.57 g/L after 60 h with a yield of 0.72 ± 0.06 g/g total sugar, approaching the predicted value (57.99 g/L). It was 1.63-fold of the SA production obtained with the basic medium (35.54 g/L). In addition, batch fermentations were carried out in a 1.3-L stirred bioreactor and SA reached 58.47 g/L. These results indicate that XML could be an alternative substrate for the economical production of SA by A. succinogenes GXAS137.
摘要本研究以木糖母液(XML)为培养基,对琥珀酸放线菌GXAS137产琥珀酸的条件进行了优化。首先,通过单因素试验对SA发酵的基础培养基进行了评价。随后,采用Plackett-Burman设计从原9个因子中筛选出XML、玉米浆粉(CSLP)和MgCO3 3个影响SA产率的显著因子。随后利用最陡爬坡实验确定了三个因素的中心面积。最后,利用响应面法进一步优化3个主要因素之间的相互作用,并通过Box-Behnken设计预测最大SA浓度。发酵SA的最佳条件为XML (110 g/L)、CSLP (18.86 g/L)和MgCO3 (69.12 g/L)。60 h后SA的最大产量为58.06±0.57 g/L,总糖产量为0.72±0.06 g/g,接近预测值(57.99 g/L)。为基础培养基(35.54 g/L)的1.63倍。在1.3 L搅拌生物反应器中分批发酵,SA达到58.47 g/L。这些结果表明,XML可以作为琥珀酸A. succinogenes GXAS137经济生产SA的替代底物。
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引用次数: 4
Enhancing precision medicine through clinical mass spectrometry platform 通过临床质谱平台提升精准医疗
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2053342
J. Hristova, D. Svinarov
Abstract There is an extraordinary flood of new technologies in medicine nowadays. Sophisticated diagnostics based on genome assays, mass spectrometry and cell sorting platforms are driving the technological transfer and promote the entrance of individualized patient management in clinical practice. Mass spectrometry (MS) could be viewed as one of the major tools that promote the development of precision medicine (PM), which employs a patient’s genotype and phenotype investigation to establish individually tailored drug treatment. While genetic testing allows the physician to choose appropriate medicine, MS assays provide the patient’s actual phenotype, with all of the environmental, pharmacological and pathological variables. Therefore, MS is an essential technology for personalized patient management, and high-resolution MS systems are employed to resolve challenging analytical demands. The great technological advance of MS resulted in the introduction of methods with unprecedented identification power, extreme sensitivity, specificity and extended linearity range, which are simpler to use in the medical laboratories, and are based on the current reference analytical principles. Further, the ability to perform panel profiling with simultaneous measurement of bioactive compounds, their precursors and metabolites in a single sample, enormously amplifies the informative value of results, with ultimate improvement of patient care. Typical examples include newborn screening, therapeutic drug management, toxicology, endocrinology, microbiology, clinical omics assays and others. It should be specially emphasized that clinical MS integrates chemical and anatomical pathology: MS imaging and iKnife-MS guidance in surgery, although still in the research phase, open new horizons for personalized treatment and individualized patient care.
摘要当今医学上出现了大量的新技术。基于基因组分析、质谱和细胞分选平台的复杂诊断正在推动技术转让,并促进个性化患者管理进入临床实践。质谱(MS)可以被视为促进精准医学(PM)发展的主要工具之一,该医学利用患者的基因型和表型调查来建立个性化的药物治疗。虽然基因检测允许医生选择合适的药物,但MS检测提供了患者的实际表型,以及所有的环境、药理学和病理学变量。因此,MS是个性化患者管理的重要技术,高分辨率MS系统用于解决具有挑战性的分析需求。MS的巨大技术进步导致引入了具有前所未有的识别能力、极高的灵敏度、特异性和扩展的线性范围的方法,这些方法在医学实验室中更容易使用,并且基于当前的参考分析原理。此外,通过在单个样本中同时测量生物活性化合物、其前体和代谢物来进行面板分析的能力,极大地提高了结果的信息价值,最终改善了患者护理。典型的例子包括新生儿筛查、治疗药物管理、毒理学、内分泌学、微生物学、临床组学分析等。需要特别强调的是,临床多发性硬化综合了化学和解剖病理学:尽管仍处于研究阶段,但多发性痴呆成像和iKnife多发性病变手术指导为个性化治疗和个性化患者护理开辟了新的视野。
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引用次数: 0
Inhibitory activity of carbon quantum dots against Phytophthora infestans and fungal plant pathogens and their effect on dsRNA-induced gene silencing 碳量子点对晚疫病和真菌植物病原体的抑制活性及其对dsRNA诱导的基因沉默的影响
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-11-21 DOI: 10.1080/13102818.2022.2146533
K. Kostov, Boika Andonova-Lilova, G. Smagghe
Abstract Carbon quantum dots (CQDs) have many potential applications due to their cell-penetrating ability, biocompatibility and tunable properties. Among a variety of characteristics, the inhibition of bacteria by CQDs is often reported. However, the effect on other microorganisms, such as plant pathogenic fungi and oomycetes, is poorly studied. Here we monitored the growth of the oomycete plant pathogen Phytophthora infestans in the presence of CQDs, as well as of another three fungal plant pathogens, namely Botrytis cinerea, Alternaria alternata and Fusarium oxysporum. Moreover, the ability of CQDs to improve gene silencing caused by exogenous dsRNA in P. infestans was studied, and the toxicity of CQDs to human keratinocytes was evaluated. Our results indicate significant inhibitory activity of CQDs against P. infestans at relatively low concentrations. In a species-specific manner and to a lesser extent, the growth of the three fungal plant pathogens was also affected. We also found that the treatment of P. infestans with naked dsRNA in vitro did not trigger gene silencing. However, the mixture of CQDs with dsRNA increased RNAi efficiency, by causing a significant reduction of the transcript levels of the target gene in developing sporangia. Finally, no cytotoxicity of the CQDs, in the concentrations active against the plant pathogens, was found.
碳量子点(CQDs)由于其细胞穿透能力、生物相容性和可调性而具有许多潜在的应用前景。在多种特性中,CQDs对细菌的抑制作用经常被报道。然而,对其他微生物的影响,如植物病原真菌和卵菌的研究很少。在CQDs存在的情况下,我们监测了卵霉菌植物病原体疫霉(Phytophthora infestans)以及另外三种植物真菌病原体Botrytis cinerea、Alternaria alternata和Fusarium oxysporum的生长情况。此外,我们还研究了CQDs改善P. infestans由外源dsRNA引起的基因沉默的能力,并评估了CQDs对人角质形成细胞的毒性。结果表明,CQDs在较低浓度下对病原菌有明显的抑制作用。以一种特定的方式,在较小程度上,三种真菌植物病原体的生长也受到影响。我们还发现,在体外用裸dsRNA处理病原菌不会引发基因沉默。然而,CQDs与dsRNA的混合物通过显著降低孢子囊发育过程中靶基因的转录水平,提高了RNAi效率。最后,在对植物病原体有活性的浓度下,没有发现CQDs的细胞毒性。
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引用次数: 1
High rate of fluoroquinolone resistant Neisseria gonorrhoeae detected by molecular surveillance of antimicrobial resistance determinants in Bulgaria 保加利亚抗微生物耐药性决定因素的分子监测检测到耐氟喹诺酮类药物的淋病奈瑟菌的高比率
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-11-11 DOI: 10.1080/13102818.2022.2146532
I. Philipova, V. Levterova, I. Simeonovski, T. Kantardjiev
Abstract Antimicrobial-resistant Neisseria gonorrhoeae is a major public health concern. The surveillance of antimicrobial resistance benefits from rapid and accurate molecular techniques in molecular diagnostics to facilitate individualised medicine and antimicrobial stewardship. To support the recommendations for empirical treatment of gonococcal infections in Bulgaria, we investigated N. gonorrhoeae-positive clinical specimens from 2018 to 2021 for the presence of genetic determinants associated with antimicrobial resistance. N. gonorrhoeae-positive samples stored at the National Center of Infectious and Parasitic Diseases during the four-year study period were retrospectively analysed by polymerase chain reaction and DNA sequencing assays for resistance determinants to fluoroquinolones, third-generation cephalosporins and macrolides. The detected determinants indicated a high rate of fluoroquinolone resistance (59%), very low level of decreased susceptibility to third-generation cephalosporins (3%) but no macrolide resistance (0%). These findings validate the utilisation of the international guidelines’ recommendations for empirical dual therapy with ceftriaxone/cefixime and azithromycin in Bulgaria. Because of the high fluoroquinolone resistance rate, ciprofloxacin should only be considered as treatment if phenotypic or molecular antimicrobial susceptibility data indicate susceptibility to ciprofloxacin. For the purposes of surveillance and individualised medicine, molecular assays for resistance determinants could complement culture-based phenotypic gonococcal antimicrobial resistance testing.
摘要抗微生物淋病奈瑟菌是一个主要的公共卫生问题。抗菌药物耐药性的监测得益于分子诊断中快速准确的分子技术,以促进个性化药物和抗菌药物管理。为了支持保加利亚对淋球菌感染的经验治疗建议,我们调查了2018年至2021年淋球菌阳性临床标本中是否存在与抗微生物耐药性相关的遗传决定因素。在四年的研究期间,通过聚合酶链式反应和DNA测序分析,对国家传染病和寄生虫病中心储存的淋病奈瑟菌阳性样本进行了回顾性分析,以确定对氟喹诺酮类、第三代头孢菌素类和大环内酯类的耐药性决定因素。检测到的决定因素表明氟喹诺酮类药物的耐药率很高(59%),对第三代头孢菌素的易感性降低的水平很低(3%),但没有大环内酯类药物耐药性(0%)。这些发现验证了国际指南建议在保加利亚使用头孢曲松/头孢克肟和阿奇霉素的经验双重治疗。由于氟喹诺酮类药物的高耐药率,只有当表型或分子抗菌药敏数据表明对环丙沙星敏感时,才应将环丙沙星视为治疗药物。为了监测和个体化医学的目的,耐药性决定因素的分子测定可以补充基于培养的表型淋球菌抗微生物耐药性测试。
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引用次数: 0
Light filtration technology for sustainable microalgal biomass production 可持续微藻生物量生产的光过滤技术
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-11-07 DOI: 10.1080/13102818.2022.2144455
E. T. Sero, N. Siziba, Tavengwa Bunhu, R. Shoko
Abstract The use of native microalgal strains, which are well adapted to local environmental conditions, for sustainable biofuels production has largely been marred by photonics-related challenges. To date, most photobioreactor systems make use of artificial sources of illumination thus increasing the overall costs of biomass production. Solar energy, although sustainable and cost-effective, is difficult to manage and control. It also contains other wavelengths which are detrimental to microalgae. Thus, this study sought to make use of spectral filters for optimal outdoor algaculture. Hence, solar energy was used in wastewater-mediated algaculture of native and imported Chlorella sp. under blue, green, red and yellow coloured spectral filters. The native Chlorella sp. had the highest growth rate of 0.892 d−1 and 0.754 d−1 under green and blue coloured filters, respectively. In comparison, the imported Chlorella strain had a growth rate of 0.379 d−1 and 0.267 d−1 under green and blue filters, respectively. Both strains produced high lipid yields under the blue coloured filter, with the native and imported Chlorella strains managing lipid yields of 41.87% dry cell weight (dcw) and 32.29% dcw, respectively. The native Chlorella strain also significantly lowered (p < 0.05) the levels of total nitrogen and ammonium from wastewater with removal efficiencies of 92.17% and 44.60%, respectively, whereas the imported Chlorella strain managed a removal efficiency of 80.81% total nitrogen and 26.10% ammonium under the blue coloured filter. The results indicate that light filtration technology can be used, sustainably, in the simultaneous algaculture of native strains and remediation of wastewater.
摘要当地微藻菌株非常适合当地环境条件,用于可持续生物燃料生产在很大程度上受到了光子学相关挑战的破坏。迄今为止,大多数光生物反应器系统使用人工照明源,从而增加了生物质生产的总成本。太阳能虽然具有可持续性和成本效益,但难以管理和控制。它还含有对微藻有害的其他波长。因此,本研究试图利用光谱滤波器进行最佳的户外藻类培养。因此,在蓝色、绿色、红色和黄色光谱滤光片下,太阳能被用于本地和进口小球藻的废水介导的藻类培养。在绿色和蓝色滤镜下,本地小球藻的生长速率最高,分别为0.892 d−1和0.754 d−1。相比之下,进口小球藻菌株在绿色和蓝色滤镜下的生长率分别为0.379 d−1和0.267 d−1。这两种菌株在蓝色滤镜下都产生了高的脂质产量,本土和进口小球藻菌株的脂质产量分别为41.87%干细胞重量(dcw)和32.29%干细胞重量。天然小球藻菌株也显著降低(p < 0.05)废水中的总氮和铵的去除率分别为92.17%和44.60%,而进口小球藻菌株在蓝色过滤器下的总氮去除率为80.81%,铵去除率为26.10%。结果表明,光过滤技术可以可持续地用于本地菌株的同时藻类培养和废水的修复。
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引用次数: 0
In vitro and in silico inhibition performance of choline against CYP1A2, CYP2D6 and CYP3A4 胆碱对CYP1A2、CYP2D6和CYP3A4的体外和体外抑制作用
IF 1.4 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2022-11-05 DOI: 10.1080/13102818.2022.2144452
E. Mateev, Antoaneta Balkanska-Mitkova, L. Peikova, M. Dimitrova, M. Kondeva-Burdina
Abstract Choline is an essential nutrient involved in the synthesis of acetylcholine in the cholinergic neurons. The pharmacokinetic properties of choline are well described; however, there is a lack of data about its activity toward the CYP450 superfamily of enzymes. Therefore, the aim of this study was to conduct in silico and in vitro activity assessments of choline against three major CYP450 isoforms—CYP1A2, CYP2D6, and CYP3A4. Preliminary in silico modeling was performed on a specialized DL-CYP Prediction Server to evaluate the affinity of choline toward the enzymes. The in vitro study contained specific cytochrome P450 isoform inhibitors and substrates (for CYP1A2, CYP2D6, and CYP3A4) to determine the inhibition performance of choline at five different concentrations (0.150 − 1 µmol/L). The potential interactions of choline and CYPs were displayed after molecular docking with Glide (Schrödinger). In addition, induced-fit simulations and binding free energy calculations MM/GBSA (Molecular Mechanics-Generalized Born Surface Area) were applied to predict the accessibility in each CYP isoform. The initial in silico simulations revealed that choline lacks inhibition potency against the aforementioned enzymes. The in vitro evaluations confirmed that choline possessed no effect against CYP1A2; however, at 1 µmol/L choline exerted 22% and 27% blocking capacity against CYP2D6 and CYP3A4, respectively. Furthermore, there was good correlation between the in vitro results and the complexes’ free binding energy recalculations. Overall, the assessments indicated that choline is a weak CYP2D6 and CYP3A4 inhibitor. The latter results should be considered as a source of future unwanted drug–drug interactions.
摘要胆碱是胆碱能神经元合成乙酰胆碱的必需营养物质。胆碱的药代动力学特性得到了很好的描述;然而,缺乏关于其对CYP450酶超家族的活性的数据。因此,本研究的目的是对胆碱对三种主要的CYP450亚型——CYP1A2、CYP2D6和CYP3A4——进行计算机和体外活性评估。在专门的DL-CYP预测服务器上进行初步的计算机建模,以评估胆碱对酶的亲和力。体外研究包含特异性细胞色素P450亚型抑制剂和底物(用于CYP1A2、CYP2D6和CYP3A4),以确定胆碱在五种不同浓度(0.150 − 1. µmol/L)。在与Glide(Schrödinger)分子对接后,显示了胆碱和CYP的潜在相互作用。此外,还应用诱导拟合模拟和结合自由能计算MM/GBSA(分子力学广义出生表面积)来预测每个CYP亚型的可及性。最初的计算机模拟显示,胆碱对上述酶缺乏抑制作用。体外评估证实胆碱对CYP1A2没有作用;但是,在1 µmol/L胆碱对CYP2D6和CYP3A4的阻断能力分别为22%和27%。此外,体外结果与复合物的自由结合能重新计算之间存在良好的相关性。总体而言,评估表明胆碱是一种较弱的CYP2D6和CYP3A4抑制剂。后一种结果应被视为未来不需要的药物-药物相互作用的来源。
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Biotechnology & Biotechnological Equipment
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