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Heterologous expression of pediocin PA-1 in Pichia pastoris: cloning, expression, characterization, and application in pork bologna preservation. 在 Pichia pastoris 中异源表达 pediocin PA-1:克隆、表达、表征及在猪肉腊肠保存中的应用。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-27 DOI: 10.1007/s42770-024-01388-w
Nguyen Pham Anh Thu, Nguyen Hieu Nghia, Dang Thi Phuong Thao, Nguyen Thi My Trinh

Objective: Pediocin PA-1, an antimicrobial peptide derived from Pediococcus acidilactici PAC1.0, has a potential application as a food preservative thanks to its strong inhibitory activity against the foodborne pathogen L. monocytogenes. This study aimed to produce Pediocin PA-1 from the yeast P. pastoris and evaluate its characteristics.

Methods: Gene encoding Pediocin PA-1 was integrated into P. pastoris X33 genome to establish the strain X33::ped, which could produce and secrete this peptide into culture medium. The antimicrobial activity of Pediocin PA-1 was examined using agar diffusion assay. The stability of pediocin PA-1 was determined based on its remaining antibacterial activity after exposure to proteases and extreme pH and temperatures. The potential use of this bacteriocin in food preservation was demonstrated using the L. monocytogenes infected pork bologna. The anticancer activity of Pediocin PA-1 was also investigated on some cancer cells using MTT assay.

Results: We established the yeast P. pastoris X33::ped capable of producing pediocin PA-1 with antimicrobial activity against L. monocytogenes and some other harmful bacteria. Pediocin PA-1 was stable at 100˚C and resistant against pH 1-12 for 1 h, but susceptible to trypsin, α-chymotrypsin, and proteinase K. This peptide could reduce the number of L. monocytogenes in pork bologna by 3.59 log CFU/g after 7 days of storage at 4˚C. Finally, Pediocin PA-1 (25 µg/ml) inhibited the proliferation of A549 and Hela cancer cells.

Conclusion: We succeeded in producing active Pediocin PA-1 from P. pastoris and demonstrated its potential use in food preservation and pharmaceutical industry.

目的:Pediocin PA-1 是一种从 Pediococcus acidilactici PAC1.0 中提取的抗菌肽,由于其对食源性病原体单核细胞增多症具有很强的抑制活性,有望用作食品防腐剂。本研究旨在从棒状酵母中生产 Pediocin PA-1,并评估其特性:方法:将编码Pediocin PA-1的基因整合到P. pastoris X33基因组中,建立了X33::ped菌株,该菌株能生产Pediocin PA-1并将其分泌到培养基中。琼脂扩散试验检验了 Pediocin PA-1 的抗菌活性。Pediocin PA-1 的稳定性是根据其暴露于蛋白酶、极端 pH 值和温度后的剩余抗菌活性来确定的。利用单核细胞增多性酵母菌感染的猪肉腊肠证明了这种细菌素在食品保鲜中的潜在用途。此外,还使用 MTT 法研究了 Pediocin PA-1 对一些癌细胞的抗癌活性:结果:我们建立了能够产生对单核细胞增多性酵母菌和其他一些有害细菌具有抗菌活性的 Pediocin PA-1 的 P. pastoris X33::ped 酵母菌。Pediocin PA-1 在 100˚C 温度下稳定,1 小时内可耐受 pH 1-12,但对胰蛋白酶、α-糜蛋白酶和蛋白酶 K 易感。最后,Pediocin PA-1(25 µg/ml)可抑制 A549 和 Hela 癌细胞的增殖:我们成功地从P.pastoris中生产出了活性Pediocin PA-1,并证明了其在食品保鲜和制药业中的潜在用途。
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引用次数: 0
Development and standardization of an enzyme-linked inmunosorbent for the detection of orthohantavirus infection in Argentina based on its bacterial-expressed nucleocapside protein. 基于细菌表达的核苷酸蛋白,开发用于检测阿根廷正泛病毒感染的酶联免疫吸附剂并使之标准化。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01412-z
Patricia M Muzulin, Julia Brignone, Néstor Gabriel Iglesias, Marcelo Rodríguez, Lucía Irazu, Jorge B García, Carina Sen, Silvana Levis

We conducted a development and standardization of an IgG ELISA assay for serological detection of human orthohantavirus infections using the recombinant antigen rLECH13 produced in bacterial and derived from the LECHV. The evaluation and standardization were carried out by analyzing serum samples from a total of 50 patients with confirmed Hantavirus Pulmonary Syndrome (HPS) diagnosis through the reference technique, 50 negative sera, and 53 patients with other medical conditions. The data from the assay analysis showed a diagnostic sensitivity value of 95% and a diagnostic specificity of 80%. The high sensitivity of this novel assay leads us to conclude that rLECH13 is a feasible option for use in the immunodiagnostic of orthohantavirus infection. Additionally, it is crucial to have an antigen that can be produced under conditions that do not require highly complex laboratories. Furthermore, the new assay is cost-effective, reproducible, and demonstrates excellent performance.

我们开发了一种 IgG ELISA 检测方法,并将其标准化,该方法使用在细菌中生产的重组抗原 rLECH13(来源于 LECHV),用于人类正汉坦病毒感染的血清学检测。评估和标准化工作是通过分析 50 名通过参考技术确诊为汉坦病毒肺综合征(HPS)的患者、50 名阴性血清和 53 名患有其他疾病的患者的血清样本进行的。化验分析数据显示,诊断灵敏度为 95%,诊断特异性为 80%。这种新型检测方法的高灵敏度使我们得出结论,rLECH13 是用于正疱疹病毒感染免疫诊断的可行选择。此外,在不需要高度复杂的实验室的条件下生产抗原也是至关重要的。此外,新的检测方法具有成本效益、可重复性和出色的性能。
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引用次数: 0
Metagenomics insight into Puga geothermal geyser located in Himalayan Geothermal Belt (Trans-Himalayan Plateau) Ladakh, India. 元基因组学对位于印度拉达克喜马拉雅地热带(跨喜马拉雅高原)的普加地热间歇泉的深入研究。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01408-9
Shalini Kumari, Geetanjli Choudhary, Kumari Anu, Sarita Devi

Puga geothermal geyser and surrounding area, located in the Himalayan Geothermal Belt of the Trans-Himalayan Plateau in Ladakh, India, are very geographically isolated and considered pristine and free of anthropogenic activities. In this study, we have conducted the first metagenomic investigation of the microbes in and around the geyser. The whole genome sequencing analysis showed the presence of a total of 44.8%, 39.7% and 41.4% bacterial phyla in the PugW, PugS, and PugSo samples respectively, 8.6% of archaeal phyla (in all the samples), unclassified (derived from other sequences, PugW: 27.6%, PugS: 27.6%, and PugSo: 15.5%) and unclassified (derived from bacteria, PugW: 12%, PugS: 13.8%, and PugSo: 13.8%). The majority of archaeal sequences were linked to Euryarchaeota (2.84%) while the majority of the bacterial communities that predominated in most geothermal locations were linked to Pseudomonadota (67.14%) and Bacteroidota (12.52%). The abundant bacterial strains at the species level included Dechloromonas aromatica, Acinetobacter baumannii, and Arcobacter butzleri, in all the samples while the most abundant archaeal species were Methanosaeta thermophile, Methanoregula boonei, and Methanosarcina berkeri. Further, this geothermal geyser metagenome has a large number of unique sequences linked to unidentified and unclassified lineages, suggesting a potential source for novel species of microbes and their products. The present study which only examined one of the many geothermal geysers and springs in the Puga geothermal area, should be regarded as a preliminary investigation of the microbiota that live in the geothermal springs on these remote areas. These findings suggest that further investigations should be undertaken to characterize the ecosystems of the Puga geothermal area, which serve as a repository for unidentified microbial lineages.

普加地热间歇泉及其周围地区位于印度拉达克跨喜马拉雅高原的喜马拉雅地热带,地理位置非常偏僻,被认为是原始的,没有人类活动。在这项研究中,我们首次对间歇泉及其周围的微生物进行了元基因组调查。全基因组测序分析表明,在 PugW、PugS 和 PugSo 样本中分别存在 44.8%、39.7% 和 41.4%的细菌门类,8.6%的古细菌门类(在 PugW 样本中)。6%)、未分类(源自其他序列,PugW:27.6%,PugS:27.6%,PugSo:15.5%)和未分类(源自细菌,PugW:12%,PugS:13.8%,PugSo:13.8%)。大多数古细菌序列与极古细菌群(2.84%)有关,而在大多数地热区占主导地位的细菌群落则与假单胞菌群(67.14%)和类杆菌群(12.52%)有关。在所有样本中,物种水平上最丰富的细菌菌株包括芳香脱氯单胞菌(Dechloromonas aromatica)、鲍曼不动杆菌(Acinetobacter baumannii)和丁兹勒弧菌(Arcobacter butzleri),而最丰富的古细菌物种则是嗜热甲烷杆菌(Methanosaeta thermophile)、沸甲烷杆菌(Methanoregula boonei)和贝克尔甲烷杆菌(Methanosarcina berkeri)。此外,该地热间歇泉元基因组有大量与未确定和未分类菌系相关联的独特序列,这表明它可能是新型微生物及其产物的来源。本研究仅考察了普加地热区众多地热间歇泉和泉眼中的一个,应被视为对生活在这些偏远地区地热泉眼中的微生物群的初步调查。这些研究结果表明,应开展进一步调查,以确定普加地热区生态系统的特征,因为该地区是未查明微生物菌系的宝库。
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引用次数: 0
Utilization of Fusarium Solani lipase for enrichment of polyunsaturated Omega-3 fatty acids. 利用 Fusarium Solani 脂肪酶富集多不饱和 Omega-3 脂肪酸。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-06-14 DOI: 10.1007/s42770-024-01411-0
Allysson Kayron de Carvalho Silva, Fernanda Jeniffer Lindoso Lima, Katia Regina Assunção Borges, Laís Araújo Souza Wolff, Marcelo Souza de Andrade, Rita de Nazaré Silva Alves, Carolina Borges Cordeiro, Marcos Antônio Custódio Neto da Silva, Maria do Desterro Soares Brandão Nascimento, Talita da Silva Espósito, Geusa Felipa de Barros Bezerra

Omega-3 fatty acids, such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), offer numerous health benefits. Enriching these fatty acids in fish oil using cost-effective methods, like lipase application, has been studied extensively. This research aimed to investigate F. solani as a potential lipase producer and compare its efficacy in enhancing polyunsaturated omega-3 fatty acids with commercial lipases. Submerged fermentation with coconut oil yielded Lipase F2, showing remarkable activity (215.68 U/mL). Lipase F2 remained stable at pH 8.0 (activity: 93.84 U/mL) and active between 35 and 70 °C, with optimal stability at 35 °C. It exhibited resistance to various surfactants and ions, showing no cytotoxic activity in vitro, crucial for its application in the food and pharmaceutical industries. Lipase F2 efficiently enriched EPA and DHA in fish oil, reaching 22.1 mol% DHA and 23.8 mol% EPA. These results underscore the economic viability and efficacy of Lipase F2, a partially purified enzyme obtained using low-cost techniques, demonstrating remarkable stability and resistance to diverse conditions. Its performance was comparable to highly pure commercially available enzymes in omega-3 production. These findings highlight the potential of F. solani as a promising lipase source, offering opportunities for economically producing omega-3 and advancing biotechnological applications in the food and supplements industry.

欧米伽-3 脂肪酸,如二十碳五烯酸(EPA)和二十二碳六烯酸(DHA),对健康有诸多益处。通过应用脂肪酶等具有成本效益的方法来丰富鱼油中的这些脂肪酸已被广泛研究。本研究旨在调查 F. solani 作为潜在脂肪酶生产者的情况,并比较其与商业脂肪酶在提高多不饱和欧米加-3 脂肪酸方面的功效。椰子油浸没发酵产生了脂肪酶 F2,显示出显著的活性(215.68 U/mL)。脂肪酶 F2 在 pH 值为 8.0 时保持稳定(活性:93.84 U/mL),在 35 至 70 °C之间具有活性,在 35 °C时稳定性最佳。它对各种表面活性剂和离子具有耐受性,在体外没有细胞毒性,这对其在食品和制药行业的应用至关重要。脂肪酶 F2 能有效富集鱼油中的 EPA 和 DHA,DHA 和 EPA 的含量分别达到 22.1 摩尔% 和 23.8 摩尔%。这些结果凸显了脂肪酶 F2 的经济可行性和功效,它是一种利用低成本技术获得的部分纯化酶,具有显著的稳定性和对各种条件的耐受性。在欧米茄-3 的生产中,它的性能可与高纯度的市售酶相媲美。这些发现凸显了 F. solani 作为一种有前途的脂肪酶来源的潜力,为经济地生产欧米伽-3 和推进食品及补充剂行业的生物技术应用提供了机会。
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引用次数: 0
Equidae pythiosis in Brazil and the world: a systematic review of the last 63 years (1960-2023). 巴西和全世界的马科焦虫病:对过去 63 年(1960-2023 年)的系统回顾。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-07-05 DOI: 10.1007/s42770-024-01435-6
Daniela Isabel Brayer Pereira, Sônia Avila Botton, Lara Baccarin Ianiski, Caroline Quintana Braga, Aline Fontanella Maciel, Luíze Garcia Melo, Cristina Gomes Zambrano, Fábio Raphael Pascoti Bruhn, Janio Morais Santurio

This systematic review compiles reports of clinical pythiosis in horses, mules and donkeys from 1960 to 2023 worldwide, focusing on Brazil. We searched databases and included 71 articles detailing clinical characteristics, geographic distribution, epidemiology, diagnostic methods, therapies, and outcomes. The results showed that publications on equine pythiosis have significantly increased since 2010. Brazil reported the highest incidence, comprising 55% of cases, predominantly in the southern, northeastern, and central-western regions during summer and autumn. Cutaneous pythiosis was the most prevalent form, generally presenting as single lesions in the appendicular region, and affected females more than males. Diagnosis typically involved histopathology, used alone or with other methods. Various treatments have been employed, with surgery, often combined with chemotherapy and immunotherapy, being the most common. Notably, 80.84% of treated animals recovered, highlighting the effectiveness of these therapies in enhancing survival rates. The limitations of the study included the lack of data in published case reports, which made it difficult to collect and calculate epidemiological data. Additionally, we recognize that pythiosis in Brazil is underreported, since this disease does not have mandatory notification and several cases are not registered and/or reported in the literature. Lastly, it is hypothesized that equid pythiosis may be more widespread than currently known, and its real occurrence in Brazil remains uncertain.

本系统综述汇编了 1960 年至 2023 年全球范围内马、骡和驴的临床脓毒血症报告,重点关注巴西。我们检索了数据库,收录了 71 篇文章,详细介绍了临床特征、地理分布、流行病学、诊断方法、疗法和结果。结果表明,自 2010 年以来,有关马焦虫病的文献显著增加。巴西的发病率最高,占病例总数的55%,主要发生在夏季和秋季的南部、东北部和中西部地区。皮肤脓毒血症是最常见的形式,一般表现为阑尾部位的单个病变,女性患者多于男性。诊断通常涉及组织病理学,可单独使用或与其他方法一起使用。治疗方法多种多样,最常见的是手术,通常结合化疗和免疫疗法。值得注意的是,80.84%接受过治疗的动物都痊愈了,这说明这些疗法在提高存活率方面非常有效。这项研究的局限性包括缺乏已发表的病例报告数据,因此难以收集和计算流行病学数据。此外,我们认识到巴西对焦虫病的报告不足,因为这种疾病没有强制通报,一些病例没有在文献中登记和/或报告。最后,我们推测马焦虫病可能比目前已知的更为普遍,其在巴西的实际发生率仍不确定。
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引用次数: 0
Long-term occurrence of multiple antimicrobial drug resistant Klebsiella pneumoniae isolates harboring virulent potential in a tertiary hospital from Brazil. 巴西一家三甲医院长期出现多种耐抗菌药物的肺炎克雷伯菌分离株,并具有致病潜能。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-14 DOI: 10.1007/s42770-024-01358-2
Ana Paula Cardoso Almeida, Miguel Augusto de Moraes, Amanda Kamyla Ferreira da Silva, Mariana Oliveira-Silva, Rafael Nakamura-Silva, Felipe Marques de Almeida, Georgios Joannis Pappas Junior, André Pitondo-Silva, Tatiana Amabile de Campos

Klebsiella pneumoniae strains are globally associated with a plethora of opportunistic and severe human infections and are known to spread genes conferring antimicrobial resistance. Some strains harbor virulence determinants that enable them to cause serious disease in any patient, both in the hospital and in the community. The aim of this study was to determine the frequency of antimicrobial resistance and virulence traits (by gene detection and string test) among 83 K. pneumoniae isolates obtained from patient cultures of a scholar tertiary hospital in the Midwestern Brazil (Brasília, DF). Antimicrobial susceptibility analysis showed that 94% (78/83) of the isolates presented one of the following resistance profiles: resistant (R, 39), multidrug-resistant (MDR, 29), or extensively drug-resistant (XDR, 10). Several MDR and XDR strains harbored multiple virulence genes and displayed hypermucoviscous phenotype. These characteristics were observed among isolates obtained throughout all the sample collection period (2013 - 2017). The K2 serotype gene, a molecular marker of hypervirulence, was detected in three isolates, one of which classified as XDR. Sequence typing revealed the occurrence of isolates belonged to high-risk (ST13) and multiple resistance-spreading clones (ST105). Thus, our findings showed the occurrence of virulent potential isolates that also presented MDR/XDR phenotypes from 2013 to 2015. This study also indicates the probable convergence of virulence and resistance since at least 2013 in Brazil.

肺炎克雷伯氏菌菌株在全球范围内与大量机会性和严重的人类感染有关,并且已知会传播产生抗菌药耐药性的基因。有些菌株具有毒力决定因子,能在医院和社区的任何病人身上引发严重疾病。本研究旨在确定巴西中西部(巴西利亚,DF)一家学术性三甲医院从患者培养物中分离出的 83 株肺炎克雷伯菌的抗菌药耐药性频率和毒力特征(通过基因检测和串联测试)。抗菌药敏感性分析表明,94%(78/83)的分离株呈现出以下耐药性特征之一:耐药(R,39 株)、多重耐药(MDR,29 株)或广泛耐药(XDR,10 株)。一些 MDR 和 XDR 菌株携带多个毒力基因,并显示出高黏液表型。在所有样本采集期间(2013-2017 年)获得的分离株中都观察到了这些特征。在三个分离株中检测到 K2 血清型基因,这是一种高毒力的分子标记,其中一个被归类为 XDR。序列分型显示,分离物属于高风险(ST13)和多重耐药性传播克隆(ST105)。因此,我们的研究结果表明,在 2013 年至 2015 年期间,出现了同时具有 MDR/XDR 表型的潜在毒力分离株。这项研究还表明,至少从 2013 年起,巴西的毒力和抗药性可能趋于一致。
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引用次数: 0
Unveiling the impact of selected essential oils on MRSA strain ATCC 33591: antibacterial efficiency, biofilm disruption, and staphyloxanthin inhibition. 揭示精选精油对 MRSA 菌株 ATCC 33591 的影响:抗菌效率、生物膜破坏和抑制钉螺黄素。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI: 10.1007/s42770-024-01374-2
Fares Elghali, Ibtissem Ibrahim, Maha Guesmi, Fakher Frikha, Sami Mnif

This work aimed to evaluate the effects of 4 selected essential oils on planktonic cells and microbial biofilms of the Staphylococcus aureus strain (MRSA ATCC 33591). The antibacterial activities of the four essential oils Geranium (Pelargonium graveolens), PgEO, Tea Tree (Melaleuca alternifolia) MaEO, Lemon peel (Citrus limon) ClEO and Peppermint (Mentha piperita) MpEO had MICs ranging from 1.56 to 12.5 µl/ml. The evaluation of the antibiofilm activities of the 4 EOs revealed that they had antiadhesive activities against S. aureus MRSA biofilms; the activity reached 60% (the EO of MpEO peppermint at a concentration of 3.12 µl/ml), and the eradication activity was 80% (the EO of PgEO and MpEO at 3.12 µl/ml). The antibiofilm activity of S. aureus has been explained by the binding of several essential oil bioactive molecules to the SarA protein, the main target protein involved in biofilm formation. The synthesis of the virulence factor staphyloxanthin by S. aureus MRSA ATCC 33591 was significantly inhibited in the presence of PgEO at a concentration of MIC/2. This inhibition was explained by the binding of the main PgEO molecules (β-citronellol and geraniol) to the CrTM protein involved in the staphyloxanthin synthesis pathway. There is evidence that these essential oils could be used as potential anti-virulents to control Staphylococcus biofilm formation.

这项研究旨在评估 4 种精油对金黄色葡萄球菌(MRSA ATCC 33591)浮游细胞和微生物生物膜的影响。四种精油天竺葵(Pelargonium graveolens)PgEO、茶树(Melaleuca alternifolia)MaEO、柠檬皮(Citrus limon)ClEO 和薄荷(Mentha piperita)MpEO 的抗菌活性 MIC 在 1.56 至 12.5 µl/ml 之间。对 4 种环氧乙烷抗生物膜活性的评估显示,它们对金黄色葡萄球菌 MRSA 生物膜具有抗粘附活性;活性达到 60%(浓度为 3.12 µl/ml 的薄荷环氧乙烷),根除活性达到 80%(浓度为 3.12 µl/ml 的苦橙环氧乙烷和薄荷环氧乙烷)。金黄色葡萄球菌的抗生物膜活性是由于几种精油生物活性分子与参与生物膜形成的主要靶蛋白 SarA 蛋白结合所致。当 PgEO 的浓度为 MIC/2 时,金黄色葡萄球菌 MRSA ATCC 33591 毒力因子短链霉素的合成受到显著抑制。这种抑制作用的原因是 PgEO 的主要分子(β-香茅醇和香叶醇)与参与短链霉素合成途径的 CrTM 蛋白结合。有证据表明,这些精油可用作潜在的抗病毒剂,以控制葡萄球菌生物膜的形成。
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引用次数: 0
Anti-obesity effects of potential probiotic Lactobacillus strains isolated from Mongolian fermented dairy products in high-fat diet-induced obese rodent model. 从蒙古发酵乳制品中分离的潜在益生菌乳酸杆菌对高脂饮食诱导的肥胖啮齿动物模型的抗肥胖作用
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-24 DOI: 10.1007/s42770-024-01372-4
Unurjargal Galindev, Uugantsetseg Erdenebold, Galindev Batnasan, Oyundelger Ganzorig, Batjargal Batdorj

This study aims to investigate the anti-obesity properties of lactic acid bacteria (LAB) isolated from fermented dairy products such as "Airag" and "Khoormog" in Mongolia. These traditional dairy products are widely used in Mongolia and believe in having potential probiotic, anti-diabetes, anti-cancer, and anti-tuberculosis properties and are made from unheated two-humped camel milk and mare milk, respectively. We chose three LAB strains based on their probiotic characteristics, including tolerance of gastric and bile acids. Then we checked the anti-obesity activity of probiotic strains in vivo. An animal model was evaluated in twenty male C57BL/6J mice by inducing obesity with a high-fat diet (HFD), which was divided into five groups: regular diet group (Negative control), HFD group (Positive control), HFD with Lacticaseibacillus paracasei X-1 (X-1), Lacticaseibacillus paracasei X-17 (X-17), and Limosilactobacillus fermentum BM-325 (BM-325). For six weeks, 5 × 109 colony-forming units (CFU) of bacteria were given orally to the LAB-fed groups. Fasting blood glucose (FBG), lipid profiles, organ index, and organ morphology were all measured. The probiotic strains suppressed growth in adipose cell volume, stabilized FBG, reduced liver cell degeneration, and slowed HFD-induced body weight gain. The results suggest that some strains increase general metabolism while lowering body weight.

本研究旨在调查从蒙古 "Airag "和 "Khoormog "等发酵乳制品中分离出的乳酸菌(LAB)的抗肥胖特性。这些传统乳制品在蒙古被广泛使用,被认为具有潜在的益生菌、抗糖尿病、抗癌和抗结核特性,分别由未经加热的双驼峰骆驼奶和母马奶制成。我们根据三种 LAB 菌株的益生特性(包括对胃酸和胆汁酸的耐受性)选择了它们。然后,我们检测了益生菌株在体内的抗肥胖活性。我们以二十只雄性 C57BL/6J 小鼠为动物模型,通过高脂饮食(HFD)诱导肥胖,并将其分为五组:普通饮食组(阴性对照组)、HFD 组(阳性对照组)、添加副乳酸杆菌 X-1 (X-1)、副乳酸杆菌 X-17 (X-17) 和 Limosilactobacillus fermentum BM-325 (BM-325) 的 HFD 组。喂食 LAB 的组别连续六周口服 5 × 109 菌落总数单位(CFU)的细菌。对空腹血糖(FBG)、血脂、器官指数和器官形态进行了测量。益生菌株抑制了脂肪细胞体积的增长,稳定了 FBG,减少了肝细胞变性,并减缓了 HFD 诱导的体重增加。结果表明,一些菌株在降低体重的同时,还能增加一般代谢。
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引用次数: 0
Influence of different culture media on the antimicrobial activity of Pediococcus pentosaceus ST65ACC against Listeria monocytogenes. 不同培养基对五味 Pediococcus ST65ACC 对李斯特菌抗菌活性的影响。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-24 DOI: 10.1007/s42770-024-01391-1
Francielly Soares Oliveira, Rafaela da Silva Rodrigues, Valéria Quintana Cavicchioli, Antônio Fernandes de Carvalho, Luís Augusto Nero

Pediococcus pentosaceus ST65ACC is a bacteriocinogenic lactic acid bacteria (LAB) isolated from Brazilian artisanal cheese that is capable of inhibiting different food pathogens, mainly Listeria monocytogenes. The production of bacteriocins can be influenced by several growth conditions, such as temperature, pH, and medium composition. This study aimed to evaluate the effect of different culture media on the production of bacteriocins and antimicrobial activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The strains were inoculated alone and in coculture in four different media: BHI broth, MRS broth, meat broth, and reconstituted skim milk (RSM) 10% (w/v). The culture media were then incubated at 37 °C for 96 h, and count analysis, pH measurement, and bacteriocin production were performed at 0, 24, 48, 72 and 96 h. L. monocytogenes was inhibited to nondetectable levels in coculture with P. pentosaceus ST65ACC in MRS broth within 96 h, consistent with the high production of bacteriocin throughout the analysis period (3,200-12,800 AU/mL). However, lower inhibitory activities of P. pentosaceus ST65ACC on L. monocytogenes Scott A were recorded in BHI, RSM, and meat broth, with low or no production of bacteriocins at the analyzed times. The composition of these culture media may have repressed the production and activity of bacteriocins and, consequently, the antagonist activity of P. pentosaceus ST65ACC on L. monocytogenes Scott A. The results showed that the antimicrobial activity was more effective in MRS broth, presenting greater production of bacteriocins and less variability when compared to the other media analyzed.

五胜肽球菌 ST65ACC 是一种从巴西手工奶酪中分离出来的细菌素乳酸菌(LAB),能够抑制不同的食品病原体,主要是单核细胞增生李斯特菌。细菌素的产生受多种生长条件的影响,如温度、pH 值和培养基成分。本研究旨在评估不同培养基对细菌素产量的影响以及戊糖杆菌 ST65ACC 对单核细胞增生李斯特菌 Scott A 的抗菌活性:BHI 肉汤、MRS 肉汤、肉汤和 10%(w/v)的重组脱脂奶(RSM)。在 MRS 肉汤中与戊糖杆菌 ST65ACC 共培养 96 小时内,单核细胞增多性乳酸杆菌被抑制到检测不到的水平,这与整个分析期间细菌素的高产量(3,200-12,800 AU/mL)是一致的。然而,在 BHI、RSM 和肉汤中,五胜肽菌 ST65ACC 对单核细胞增多性乳酸杆菌 Scott A 的抑制活性较低,在分析时间内细菌素产量较低或没有产量。这些培养基的成分可能抑制了细菌素的产生和活性,从而抑制了戊糖菌 ST65ACC 对单核细胞增多性梭菌斯科特 A 的拮抗活性。
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引用次数: 0
MIG1, TUP1 and NRG1 mediated yeast to hyphal morphogenesis inhibition in Candida albicans by ganciclovir. 更昔洛韦通过 MIG1、TUP1 和 NRG1 介导抑制白色念珠菌的酵母至芽胞形态发生。
IF 2.1 4区 生物学 Q3 MICROBIOLOGY Pub Date : 2024-09-01 Epub Date: 2024-05-24 DOI: 10.1007/s42770-024-01344-8
Tanjila Gavandi, Shivani Patil, Sargun Basrani, Shivanand Yankanchi, Sayali Chougule, S Mohan Karuppayil, Ashwini Jadhav

Candida albicans is a polymorphic human fungal pathogen and the prime etiological agent responsible for candidiasis. The main two aspects of C. albicans virulence that have been suggested are yeast-to-hyphal (Y-H) morphological transitions and biofilm development. Anti-fungal agents targeting these virulence attributes enhances the antifungal drug development process. Repositioning with other non-fungal drugs offered a one of the new strategies and a potential alternative option to counter the urgent need for antifungal drug development. In the current study, an antiviral drug ganciclovir was screened as an antifungal agent against ATCC 90028, 10231 and clinical isolate (C1). Ganciclovir at 0.5 mg/ml concentration reduced 50% hyphal development on a silicon-based urinary catheter and was visualized using scanning electron microscopy. Ganciclovir reduced ergosterol biosynthesis in both strains and C1 isolate of C. albicans in a concentration-dependent manner. Additionally, a gene expression profile study showed that ganciclovir treatment resulted in upregulation of hyphal-specific repressors MIG1, TUP1, and NRG1 in C. albicans. Additionally, an in vivo study on the Bombyx mori silkworm model further evidenced the virulence inhibitory ability of ganciclovir (0.5 mg/ml) against C. albicans. This is the first report that explore the novel anti-morphogenic activities of ganciclovir against the pathogenic C. albicans strains, along with clinical isolates. Further, ganciclovir may be considered for therapeutic purpose after combinations with standard antifungal agents.

白念珠菌是一种多态人类真菌病原体,也是念珠菌病的主要病原体。白念珠菌毒力的两个主要方面是酵母-蘑菇(Y-H)形态转换和生物膜发育。针对这些毒力特性的抗真菌药物可促进抗真菌药物的开发进程。使用其他非真菌药物进行重新定位是一种新策略,也是应对抗真菌药物开发迫切需求的潜在替代选择。本研究筛选了一种抗病毒药物更昔洛韦(ganciclovir)作为抗真菌药物,用于抗 ATCC 90028、10231 和临床分离株(C1)。0.5毫克/毫升浓度的更昔洛韦可减少硅基导尿管上50%的真菌生长,并通过扫描电子显微镜进行观察。更昔洛韦以浓度依赖的方式减少了两种菌株和白僵菌 C1 分离物的麦角甾醇生物合成。此外,一项基因表达谱研究表明,更昔洛韦处理会导致白僵菌中芽胞特异性抑制因子 MIG1、TUP1 和 NRG1 的上调。此外,对家蚕模型的体内研究进一步证明了更昔洛韦(0.5 mg/ml)对白僵菌的毒力抑制能力。这是第一份探讨更昔洛韦对致病性白僵菌菌株和临床分离株的新型抗变态反应活性的报告。此外,更昔洛韦与标准抗真菌药物联用后,可考虑用于治疗目的。
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引用次数: 0
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Brazilian Journal of Microbiology
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