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Isolation of pathogenic microorganisms from fresh fruits and screening the efficacy of different disinfectant solution against the pathogens 新鲜水果中病原微生物的分离及不同消毒液对病原菌的抑菌效果的筛选
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6945
I. Nur
Fruits are important parts of our daily diet to maintain a healthy life as they have potential nutritional values. But unfortunately, a number of foodborne diseases have been noticed due to the consumption of raw and contaminated fruits. The present study was carried out to isolate the pathogenic microorganisms from fruits sample and to determine the effects of some household cleaning methods including washing and various common chemical treatments for the removal of bacterial load. A conventional spread plate technique was performed for the detection of bacteria. Total heterotrophic bacteria, Escherichia coli, Staphylococcus spp. Salmonella spp. and Listeria spp. were found in 30 samples of 6 categories including Java apple (Syzygium cumini), Carambola (Averrhoa carambola), Indian gooseberry (Phyllanthus emblica), Olive (Olea europaea) Koromcha (Carissa carandas) and Pear (Pyrus). Tap water, hot water (50°C), 100 mg/L sodium hypochlorite (NaOCl), 50 mg/L calcium lactate, 4% acetic acid and 2 ml/L CleanAva were used as decontaminating agents. All samples were soaked in tested cleaning agents for 20 min at room temperature. All the tested solutions were found to be effective and reduced bacterial loads in fruits compared to the unwashed fruits samples (p<0.01). It was revealed, NaOCl, calcium lactate, acetic acid and CleanAva were more effective cleaning agents than water wash. Two to three log of bacterial load was reduced when samples were subjected to treat with decontaminating agents. Potable water, the types and concentration of the disinfectant solutions are important parameters for effective washing.
水果是我们日常饮食中保持健康生活的重要组成部分,因为它们具有潜在的营养价值。但不幸的是,由于食用生的和受污染的水果,已经注意到一些食源性疾病。本研究旨在从水果样品中分离病原微生物,并确定一些家庭清洁方法,包括洗涤和各种常见的化学处理对细菌负荷的去除效果。采用常规平板法进行细菌检测。在Java苹果(Syzygium cumini)、杨桃(Averrhoa Carambola)、印度醋栗(Phyllanthus emblica)、橄榄(Olea europaea)、卡瑞萨(Carissa carandas)和梨(Pyrus) 6类30份样品中检出大肠杆菌、葡萄球菌、沙门氏菌和李斯特菌。以自来水、热水(50℃)、次氯酸钠(NaOCl) 100 mg/L、乳酸钙50 mg/L、乙酸4%、CleanAva 2 ml/L作为去污剂。所有样品在室温下用所测清洗剂浸泡20分钟。与未清洗的水果样品相比,所有被测试的溶液都有效,并且减少了水果中的细菌负荷(p<0.01)。结果表明,NaOCl、乳酸钙、乙酸、CleanAva等清洗剂比水洗效果更好。当样品受到去污剂处理时,细菌负荷减少了2至3 log。饮用水、消毒液的种类和浓度是有效洗涤的重要参数。
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引用次数: 0
Assessment of microbial quality of industrial and traditional creams in Alborz province, Iran 伊朗Alborz省工业面霜和传统面霜微生物质量评价
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6947
Monica Aghvami, G. J. Khaniki, S. Shokri, N. Jalali
Milk and dairy products play an important role in the human diet and society's health. The aim of this study was the assessment of the microbial quality of industrial and traditional breakfast cream in Alborz province, Iran. In this study, 40 different samples of breakfast cream (20 samples of traditional breakfast cream and 20 samples of industrial pasteurized breakfast cream) were collected randomly in Alborz province in 2018. Microbial quality tests were performed according to Iran National Standards on Coliforms, Escherichia coli, and Staphylococcus aureus, and then the collected data were analyzed. The microbiological examinations revealed that 43% of the samples were contaminated with coliform bacteria that 12 samples (60%) out of 20 samples of traditional cream, 5 samples (25%) out of 20 samples of industrial cream were higher than the allowable microbial limit of the national standard of Iran. About 15% of samples of traditional creams and 10% of industrial creams were contaminated with Escherichia coli. 10% of samples of traditional cream were contaminated with Staphylococcus aureus, which was not observed in industrial creams. High contamination with bacteria, needs using different methods to control microbial growth, including the promotion of sanitary awareness among laborers, the codification of microbial standards for traditional dairy products, training to staff for preparing the cream and disinfection of tools.
牛奶和奶制品在人类饮食和社会健康中起着重要作用。本研究的目的是评估伊朗阿尔博尔斯省工业和传统早餐奶油的微生物质量。在本研究中,2018年在阿尔博尔斯省随机收集了40种不同的早餐奶油样品(20种传统早餐奶油和20种工业巴氏杀菌早餐奶油)。按照伊朗大肠菌群、大肠杆菌和金黄色葡萄球菌国家标准进行微生物质量检测,并对采集数据进行分析。微生物学检测结果显示,43%的样品被大肠菌群污染,20个传统乳膏样品中有12个样品(60%),20个工业乳膏样品中有5个样品(25%)的微生物含量高于伊朗国家标准允许的微生物限量。约15%的传统面霜样品和10%的工业面霜样品被大肠杆菌污染。10%的传统乳膏样品被金黄色葡萄球菌污染,而在工业乳膏中未发现这种情况。细菌高度污染,需要使用不同的方法来控制微生物的生长,包括提高劳动者的卫生意识,对传统乳制品的微生物标准进行编纂,对员工进行乳霜准备和工具消毒的培训。
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引用次数: 1
Microbiological quality of street vended Panipuri in Jagtial district of Telangana, India 印度特伦甘纳邦贾格提尔区街头售卖的帕尼普里的微生物质量
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6944
Venkatesh Teegala, Kanakamedala Bipin Chandra Pavan, Anumolu Vijaya Kumar
Street vended foods have gained a lot of popularity in recent days owing to their appealing taste and flavour. These foods apart from the taste and flavour, have been cost-effective and easily available for all people. That has been the reason for such high demand for these foods in all walks of life and are being relished by the populations from the rural to urban areas. Street foods have been satisfying the hunger of a good percentage of people at an affordable cost. On the other side of the situation, these foods have been posing a good risk of health problems for the people because of the unhygienic methods and process of preparation of these foods by the mostly illiterate community of businessmen who have been preparing them. Hence the need of evaluating these foods for the contamination levels and risk factors for human health have been exponentially felt and the present study was taken up. Therefore, the present study was carried out to check the microbiological quality of street vended panipuri sold in Korutla, Metpally, and Jagtial towns of Jagtial Dist, Telangana state. A total of ninety-six samples viz., water, dall and kachori (thirty two each) were aseptically collected from various vendors and were subjected for standard plate count, total Coliform count, total Salmonella count and total Staphylococcus aureus count. Results revealed that 74% of samples had high loads of bacterial pathogens such as Escherichia coli (41%), Staphylococcus aureus (31%), sp. (20%), Pseudomonas sp. (5%) and yeast (3%). Hence, it has been enumerated that the quality of street foods must be monitored, and standards of the microbiological load have to be followed for human health safety.
最近几天,街头小吃因其诱人的味道和风味而大受欢迎。这些食物除了味道和味道外,还具有成本效益,而且所有人都可以轻松获得。这就是各行各业对这些食物需求如此之高的原因,从农村到城市,人们都在享用这些食物。街头食品以可承受的成本满足了相当一部分人的饥饿感。另一方面,这些食品对人们的健康构成了很大的风险,因为这些食品的制备方法和过程不卫生,而这些食品的大部分是文盲商人。因此,人们普遍认为有必要对这些食品的污染水平和人类健康的风险因素进行评估,并开始了本研究。因此,本研究旨在检查特伦甘纳州贾格蒂亚尔区Korutla、Metpally和贾格蒂阿尔镇街头出售的帕尼普里的微生物质量。共从不同供应商无菌收集了96个样本,即水、dall和kachori(各32个),并进行了标准平板计数、大肠杆菌总数、沙门氏菌总数和金黄色葡萄球菌总数。结果显示,74%的样本具有高载量的细菌病原体,如大肠杆菌(41%)、金黄色葡萄球菌(31%)、假单胞菌(20%)、假单孢菌(5%)和酵母(3%)。因此,为了人类健康安全,必须监测街头食品的质量,并遵守微生物负荷标准。
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引用次数: 1
A clinical epidemiological onsite study of a massive outbreak of Scombroid fish poisoning after consumption of yellowtail kingfish in northern Chile 智利北部食用黄尾王鱼后大规模爆发鲭鱼中毒的临床流行病学现场研究
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6946
R. Zapata, K. Acevedo, M. Mella, Verena Mella, Keryma Zapata
Scombroid fish poisoning (SFP) is an acute illness caused by the consumption of poorly preserved fish containing high levels of histamine. Symptoms develop within 1-2 h, with gastrointestinal, dermatologic, and cardiopulmonary manifestations. The diagnosis relies exclusively on the clinical picture. To describe an onsite clinical-epidemiological study of a massive outbreak of SFP occurring in Chile. Descriptive study of an outbreak occurring in a closed Convention Center after lunch with Chilean Palometa or Seriola lalandi (Yellowtail kingfish). An Onsite questionnaire was answered by all attendants within 24 h. Local health officials supervised the place with a second survey and sample recollection of suspected fish. Eighty-three adults attended the event, of which 81 ate fish. Mean age: 58.5 years old, 82.7% women. The symptomatic attack rate of people eating suspected poisoned fish was 79% (64 out of 81 participants). Most common symptoms included: diarrhea (68%); headache (64%); flushing (64%) and diffuse redness (56%). Mean incubation period: 77 min (30-240 min). 98.4% of patients recovered within 10 h. Ten patients were referred to the emergency room, but none were admitted. There was a presumption of loss of refrigeration in the handling of fish, and confirmation of SFP by clinical basis, incubation period and attack rate. Fish was the only food item associated with illness (Odds ratio: 19, p = 0.014; Fisher two tails). This is one of the 5 largest outbreaks of SFP with fresh fish, ever reported in the literature. The clinical picture and rapid onset of symptoms made it possible to suspect SFP allowing timely management of patients. It is relevant to spread the knowledge about this underdiagnosed and underreported syndrome.
鲭鱼中毒(Scombroid fish poisoning, SFP)是一种急性疾病,是由于食用了含有高水平组胺的保存不良的鱼而引起的。症状在1-2小时内出现,伴有胃肠道、皮肤和心肺症状。诊断完全依靠临床表现。描述智利发生的一次大规模SFP暴发的现场临床流行病学研究。在封闭的会议中心与智利Palometa或serola lalandi(黄尾王鱼)共进午餐后发生的一次暴发的描述性研究。所有工作人员在24小时内回答了现场问卷。当地卫生官员对该场所进行了第二次调查,并回收了可疑鱼类的样本。83名成年人参加了这次活动,其中81人吃了鱼。平均年龄:58.5岁,82.7%为女性。在81名参与者中,有64人食用了疑似中毒的鱼类,症状发作率为79%。最常见的症状包括:腹泻(68%);头痛(64%);潮红(64%)和弥漫性发红(56%)。平均潜伏期:77分钟(30-240分钟)。98.4%的患者在10小时内康复。10名患者被转介到急诊室,但没有人住院。在处理鱼的过程中有冷藏损失的推定,并通过临床基础、潜伏期和发病率确认SFP。鱼是唯一与疾病相关的食物(优势比:19,p = 0.014;两条尾巴)。这是文献中报道过的5次最大的鲜鱼SFP爆发之一。临床表现和快速发作的症状使人们有可能怀疑SFP,从而及时对患者进行治疗。传播关于这种未被充分诊断和报告不足的综合征的知识是有意义的。
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引用次数: 1
Evaluation of Escherichia coli contamination of consumed eggs around the campus of the agricultural state Polytechnic of Payakumbuh, Indonesia 印度尼西亚巴亚昆布农业州立理工学院校园周围食用鸡蛋的大肠杆菌污染评估
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6943
Kevin Akvia Pratama, Prima Silvia Noor, Engki Zelpina, Sujatmiko
Escherichia coli (E. coli) is a pathogen that is enteropathogenic and/or toxic to human health. In addition, it is also a food safety indicator and an indicator of sanitary conditions for food of animal origin. This study aims to quantify E. coli in consumptive eggs in chicken coops around the Agricultural State Polytechnic of Payakumbuh University campus. A total of 30 samples of eggs consumed for food were taken from 5 cooperatives around the campus of the Agricultural State Polytechnic of Payakumbuh. Testing the amount of E. coli in eggs using the total bacterial count (TPC) method, the average E. coli count was 1.9 x 106 cfu/mL. The existence of E. coli illustrates the contamination of chicken eggs from laying hens around the campus Agricultural State Polytechnic of Payakumbuh, the need to maintain sanitation and biosecurity measures in keeping laying hens so that they produce eggs that are safe and sound suitable for community consumption.
大肠杆菌(E. coli)是一种肠致病性和/或对人体健康有毒的病原体。此外,它也是食品安全指标和动物源性食品卫生条件的指标。本研究旨在量化Payakumbuh大学农业州立理工学院校园周围鸡舍中食用鸡蛋中的大肠杆菌。从Payakumbuh国立农业理工学院校园周围的5个合作社共采集了30个食用鸡蛋样本。采用总细菌计数法(TPC)检测鸡蛋中大肠杆菌的数量,平均大肠杆菌计数为1.9 × 106 cfu/mL。大肠杆菌的存在说明了帕亚库姆布农业州立理工学院校园周围的蛋鸡对鸡蛋的污染,以及在饲养蛋鸡方面保持卫生和生物安全措施的必要性,以便它们生产出适合社区消费的安全可靠的鸡蛋。
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引用次数: 0
The effect of food processing on the amount of trace elements and their bioavailability: a review 食品加工对微量元素含量及其生物利用度的影响
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6520
M. Neyestani, Parisa Shavali-Gilani, M. Fesahat, E. Molaee-aghaee, N. Shariatifar
Trace elements are compounds that are essential in small amounts for biochemical reactions and to maintain human health. Almost all foods can contain varying amounts of these metals. In this study, the effects of food processing on the content of trace metals are investigated. Extensive interpretations of processing, including aspects of food production and specific examples of changes in metal content due to processing will be discussed. Pre-consumption food processing to improve rheological properties and increase shelf life is inevitable, which changes the bioavailability and amount of these compounds in different directions depending on the process. The amount of these trace metals in the food product can be affected by various conditions such as heating, fermentation, food additives, etc. The main factor in reducing trace elements in food, especially the use of heat in a special method and on the other hand, factors such as fermentation can also increase the bioavailability of these elements.
微量元素是生物化学反应和维持人体健康所必需的微量化合物。几乎所有的食物都含有不同数量的这些金属。本研究探讨了食品加工对微量金属含量的影响。将讨论对加工的广泛解释,包括食品生产的各个方面以及由于加工而导致金属含量变化的具体例子。食用前食品加工以改善流变性能和延长保质期是不可避免的,这会根据加工过程在不同方向上改变这些化合物的生物利用度和数量。这些微量金属在食品中的含量会受到加热、发酵、食品添加剂等各种条件的影响。减少食品中微量元素的主要因素,特别是用特殊的方法加热,另一方面,发酵等因素也可以增加这些元素的生物利用度。
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引用次数: 2
Exposure to acetaldehyde through food; a carcinogenic agent 通过食物接触乙醛;致癌物质
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6525
P. Sadighara, A. Mohajer, E. Molaee-aghaee, Mohammad Zirak
The article's abstract is not available.
这篇文章的摘要没有。
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引用次数: 0
Knowledge, attitudes and practice of food services staff on food hygiene at Tabriz University of Medical Sciences, Iran 伊朗大不里士医学科学大学食品服务人员的食品卫生知识、态度和做法
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6524
Nasim Zolfaghari Firouzsalari, Arezou Khezerlou, H. Zolfaghari, Najmeh Shahmahdi, Mahmood Alizadeh-Sani, Z. Mahmoodi
Food hygiene must be observed in production, storage, supply, and sales to obtain healthy foods with desirable quality. The purpose of present study was to evaluate the awareness, attitudes, and practices of Tabriz University of Medical Sciences food services staff toward food hygiene. A questionnaire consisting of 46 questions was completed by 40 food services staff from February to March 2019. Based on the results, respondents had an acceptable awareness (92.5% to 100% general sanitary awareness and 37.5% to 95% awareness about microbial food hazards), excellent attitudes (97.5% to 100%), and moderate practices regarding food hygiene (17.5% to 97.5%). Although all participants had a relatively high level of awareness and attitude and participated in food safety education courses, their practices were somewhat poor.
从生产、储存、供应、销售等各个环节都必须遵守食品卫生,才能获得健康、优质的食品。本研究的目的是评估大不里士医科大学食品服务人员对食品卫生的意识、态度和实践。2019年2月至3月,40名食品服务人员完成了一份包含46个问题的问卷。根据调查结果,受访者对食品卫生的认识(92.5% ~ 100%的一般卫生认识,37.5% ~ 95%的食品微生物危害认识)尚可,对食品卫生的态度优良(97.5% ~ 100%),对食品卫生的做法一般(17.5% ~ 97.5%)。虽然所有的参与者都有较高的意识和态度,并参加了食品安全教育课程,但他们的做法有些差。
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引用次数: 0
Evaluation of natural and synthetic dyes in saffron barbecued chicken collected from meat shops and restaurants in Babol using thin-layer chromatography 用薄层色谱法评价巴勃尔肉店和餐馆采集的藏红花烤鸡中的天然和合成染料
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6522
Atiyeh Bolbol Amir, R. Partovi, Ebrahim Javan Amoli, F. Tooryan
Synthetic colors have advantages in comparison to natural colors, but they cause cancer, attentiondeficit hyperactivity disorder, immune system suppression and vitamin deficiency. In the present study, authorized and unauthorized synthetic colors in saffron barbecued chicken from meat shops and restaurants in Babol city have been identified by thin-layer chromatography. Fifty samples of saffron barbecued chicken from meat shops and restaurants in Babol city have been collected from July to September 2019. Out of 50 samples, 20 had natural color, while 27 and 3 samples contained authorized and unauthorized synthetic colors respectively. According to the Institute of Standards and Industrial Research of Iran, the application of synthetic colors is banned in saffron barbecued chicken. Therefore, 40% were consumable and 60% were inconsumable, out of which 54% contained quinoline yellow and 6% had tartrazine. About 58.06% and 63.15% of samples from meat shops and restaurants were inconsumable, respectively. There were no significant differences regarding the frequency of the evaluated colors between samples from meat shops and restaurants (p>0.05). Based on the results, it is essential that the presence of synthetic colors is traced constantly and more strictly in food products and that the perpetrators receive more serious punishment.
与天然色素相比,合成色素具有优势,但它们会导致癌症、注意力缺陷多动障碍、免疫系统抑制和维生素缺乏。本研究采用薄层色谱法对巴宝勒市肉店和餐馆藏红花烤鸡中授权和未经授权的合成色素进行了鉴定。2019年7月至9月,从巴宝勒市的肉店和餐馆收集了50份藏红花烤鸡样本。在50个样品中,20个样品含有天然色素,27个样品含有授权合成色素,3个样品含有未经授权合成色素。根据伊朗标准和工业研究所的规定,在藏红花烤鸡中禁止使用合成色素。因此,40%为消耗品,60%为不可消耗品,其中54%含喹啉黄,6%含酒黄石。来自肉店和食肆的样本分别约58.06%和63.15%为不可食用的。在肉店和餐馆的样品中,评估颜色的频率没有显著差异(p < 0.05)。根据研究结果,有必要对食品中合成色素的存在进行不断和更严格的追查,并对肇事者进行更严厉的惩罚。
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引用次数: 0
The occurrence of multidrug-resistant Salmonella nagoya and other serovars in healthy commercial layers in Ilorin, Nigeria 尼日利亚伊洛林市健康商品蛋鸡中耐多药名古屋沙门氏菌及其他血清型的发生
Pub Date : 2021-06-23 DOI: 10.18502/jfsh.v6i2.6523
A. Al-Mustapha, Raufu A. Ibrahim, Y. Adesiji, A. Heikinheimo, V. Adetunji
The occurrence of drug-resistant non-typhoidal Salmonella (NTS) in poultry has serious economic implications for the poultry industry and has the potential to cause human Salmonellosis. This study, therefore, aimed to determine the circulating serovars of NTS and their antibiotic susceptibility patterns in poultry in Ilorin. This cross-sectional study was conducted from January to March 2015. A total of 420 samples (cloacal, n=140; fecal, n=140; feed, n=70, and water, n=70) were aseptically collected from live adult birds from 14 farms using a systematic random sampling technique. Salmonella was isolated using the ISO 6579 method. Antibiotic sensitivity testing of NTS serovars was performed using the Kirby Bauer disc – diffusion method and interpreted using the epidemiological cut-off (ECOFF) values. The prevalence of NTS in poultry was 7.4% (n=31). Feed samples were the most contaminated samples (42%, n=13/31). Faecal sample (32%, n=10/31), cloacal swabs (19.5%, n= 6/31), and water samples (6.5%, n=2/31) also contained NTS. There was a significant difference between NTS isolation rates between farms (p<0.05). Only 21 isolates purposively selected across farms and sample types were serotyped. Salmonella nagoya was the most prevalent (52%, n=11/21). Other serovars were Salmonella brijbhumi (5%, n=1/21); Salmonella enteritidis (5%, n=1/21); and Salmonella enterica subsp. enterica serovar 6, 8: z4 (19%, n=4/21). Four isolates (19%) were untypable. All isolates showed multidrug resistance. Most of the isolates were resistant to ampicillin (82.3%) and tetracycline (76.5%). Some isolates were resistant to cefotaxime (23.5%) and ciprofloxacin (29.4%). The occurrence of multidrug-resistant salmonella isolates is considered a critical public health threat that requires urgent global action. There is a need for a coordinated national salmonella surveillance program in Nigeria.
家禽中出现耐药性非伤寒沙门氏菌(NTS)对家禽业具有严重的经济影响,并有可能导致人类沙门氏菌病。因此,本研究旨在确定伊洛林地区家禽中NTS的循环血清型及其抗生素易感性模式。这项横断面研究于2015年1月至3月进行。采用系统随机抽样技术,从14个养殖场的成年活禽中无菌采集了420个样本(泄殖腔,n=140;粪便,n=140,饲料,n=70,水,n=70)。沙门氏菌是使用ISO6579方法分离的。NTS血清型的抗生素敏感性测试使用Kirby-Bauer圆盘扩散法进行,并使用流行病学截止值(ECOFF)进行解释。NTS在家禽中的患病率为7.4%(n=31)。饲料样品是污染最严重的样品(42%,n=13/31)。粪便样本(32%,n=10/31)、泄殖腔拭子(19.5%,n=6/31)和水样(6.5%,n=2/31)也含有NTS。不同养殖场的NTS分离率之间存在显著差异(p<0.05)。只有21个分离株是在不同养殖场和样本类型中有意选择的。名古屋沙门氏菌最为流行(52%,n=11/21)。其他血清型为brijbhumi沙门氏菌(5%,n=1/21);肠炎沙门氏菌(5%,n=1/21);和肠炎沙门氏菌亚种。肠炎血清型6,8:z4(19%,n=4/21)。4个分离株(19%)不可分型。所有分离株均表现出多药耐药性。大多数分离株对氨苄青霉素(82.3%)和四环素(76.5%)具有耐药性。一些分离株对头孢噻肟(23.5%)和环丙沙星(29.4%)具有抗性。耐多药沙门氏菌分离株的出现被认为是一个严重的公共健康威胁,需要紧急采取全球行动。尼日利亚需要一个协调一致的全国沙门氏菌监测计划。
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引用次数: 0
期刊
Journal of food safety and hygiene
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