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Assessment of hygiene status of poultry slaughtering facilities and meat handling practices of butchers by using a hygiene assessment tool 使用卫生评估工具评估家禽屠宰设施的卫生状况和屠夫的肉类处理做法
Pub Date : 2021-11-27 DOI: 10.18502/jfsh.v7i1.7843
Sadaf Tagar, Naveed Ahmed
In the present study, hygiene conditions and meat handling practices of poultry butchers are assessed by using a hygiene assessment tool. The study was conducted in the Hyderabad and Jamshoro districts of Pakistan from April 2019 to February 2020. A total of fifty slaughtering facilities were selected based on convenient sampling techniques and butchers from those shops were interviewed by using a hygienic assessment tool. Moreover, socio-demographic details of butchers were also recorded by questionnaire survey. The hygiene assessment tool comprises three domains, personal hygiene index (PHI), meat hygiene index (MHI) and slaughtering facility hygiene index (SFHI). Each of the indexes consists of certain observations and each of the positive observations was scored one, while negative observation was scored zero. It has been revealed from this study that more than 50% of butchers were without any education and none of the butchers had received any formal training in slaughtering and meat handling practices. Basic prerequisites of hygiene such as handwashing facilities and clean water were missing in all of the slaughtering facilities. More than 30 and 50 percent of butchers scored the least (between zero – one) in PHI MHI and SFHI. However, the total score of butchers with a higher level of education was greater (p < 0.05) than with a lower education level. To improve hygiene levels and reduce transmission of diseases due to the consumption of contaminated meat, regular surveillance of the poultry shops and formal training of butchers are necessary.
在本研究中,使用卫生评估工具对家禽肉店的卫生条件和肉类处理做法进行了评估。这项研究于2019年4月至2020年2月在巴基斯坦海得拉巴和詹绍罗地区进行。根据方便的抽样技术,共选择了50家屠宰厂,并使用卫生评估工具采访了这些屠宰厂的屠夫。此外,还通过问卷调查记录了屠夫的社会人口统计细节。卫生评估工具包括三个领域,即个人卫生指数、肉类卫生指数和屠宰设施卫生指数。每个指标都由某些观察结果组成,每个阳性观察结果都得一分,而阴性观察结果得零分。这项研究表明,超过50%的屠夫没有受过任何教育,也没有一名屠夫接受过任何屠宰和肉类处理实践方面的正式培训。所有屠宰设施都缺少洗手设施和清洁水等基本的卫生先决条件。超过30%和50%的屠夫在PHI MHI和SFHI中得分最低(在零到一之间)。然而,受教育程度较高的屠夫的总分高于受教育程度较低的屠夫(p<0.05)。为了提高卫生水平,减少因食用受污染肉类而导致的疾病传播,有必要对家禽店进行定期监测,并对屠夫进行正式培训。
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引用次数: 2
Measuring the levels of Zn, Cu, Pb and Cd via the polarography method in fermentative pickled cucumbers purchased from Tehran market, Iran 用极谱法测定产自伊朗德黑兰市场的发酵腌黄瓜中锌、铜、铅和镉的含量
Pub Date : 2021-11-27 DOI: 10.18502/jfsh.v7i1.7844
B. Jannat, Sara Mirshamsi, N. Sadeghi, M. Oveisi, M. Hajimahmoodi
Pickled cucumber is frequently consumed by Iranians. According to the production process, there are two forms of pickled cucumber including industrial and fermentative. There is not an appropriate monitoring system available for the production of the latter form; therefore, high levels of heavy metals might be present in this type of pickled cucumber. Accordingly, so the levels of cadmium, zinc, lead and copper were measured in fermentative pickled cucumbers obtained from the Tehran market via the polarographic method. Polarography is a subclass of voltammetry where the working electrode is a dropping mercury electrode (DME) or a static mercury drop electrode (SMDE), which are useful for their wide cathodic ranges and renewable surfaces. Fifty different samples of pickled cucumbers were purchased from the market. Of each sample, dry ashes were produced. Measurement was repeated three times and the acquired data was then analyzed. The mean levels of zinc and copper in the evaluated samples were significantly lower than the standard limits respectively presented by the EOS and Codex (P value<0.001) while the mean level of cadmium and lead was slightly higher than the standard limit established by Codex with no significant differences observed (P values=0.450 and 0.246, respectively).
伊朗人经常食用腌黄瓜。根据生产工艺,腌黄瓜有两种形式,包括工业腌黄瓜和发酵腌黄瓜。没有适当的监测系统可用于后一种形式的生产;因此,在这种类型的腌黄瓜中可能存在高水平的重金属。因此,通过极谱法测定了从德黑兰市场获得的发酵腌黄瓜中的镉、锌、铅和铜的含量。极谱法是伏安法的一个子类,其中工作电极是滴汞电极(DME)或静态汞滴电极(SMDE),这对其宽的阴极范围和可再生表面很有用。从市场上购买了50种不同的腌黄瓜样品。在每个样品中,都产生了干灰。重复测量三次,然后对采集的数据进行分析。评估样品中锌和铜的平均水平显著低于EOS和Codex分别规定的标准限值(P值<0.001),而镉和铅的平均水平略高于Codex规定的标准限量,没有观察到显著差异(P值分别为0.450和0.246)。
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引用次数: 0
Potential application of essential oil of Lavandulastoechas in poultry meat during refrigerated storage Lavandulastechas精油在禽肉冷藏中的潜在应用
Pub Date : 2021-11-27 DOI: 10.18502/jfsh.v7i1.7846
J. Sriti, M. Boulares, Youkabed Zarroug, R. Essid, Nadia Farès, Sélim Jallouli, Saida Djebbi, N. Salem
The inhibitory effects of essential oil (EO) on bacteria development give them an important role in the fields of the food industry as an additive in food packaging. This study was aimed to identify the effect of Lavandula stoechas EO at different concentrations on chicken fillets quality during refrigerated storage. Antimicrobial and antioxidant activities showed that EO extracted has an important antibacterial activity and antiradical potential. In chicken fillets, the lavender EO (100 and 200 ppm) reduced their oxidation and microbial proliferation during refrigerated storage and with no cytotoxicity effect towards murine macrophage cells. During the storage period, the values of pH, dry matter, acidity and cooking loss of treated fillets were lower than that of the control. The statistical analyses proved greatly significant variations of color between the control and the treated by LEO, during storage. The sensory analysis selected an improvement effect in the organoleptic quality of the chicken meat when it was supplemented by 100 ppm of LEO. The statistical analysis of the microbiological characteristics clearly discriminated the control and those treated with LEO (p<0.05). The effectiveness of LEO was proved by its incorporation as a natural food preservative and the improvement of the shelf life of poultry meat products by about 3 days.
精油对细菌生长的抑制作用使其作为食品包装添加剂在食品工业领域发挥着重要作用。本研究旨在研究不同浓度的熏衣草精油对冷藏过程中鸡柳品质的影响。抑菌活性和抗氧化活性表明,精油提取物具有重要的抑菌活性和抗自由基活性。熏衣草EO(100和200 ppm)在鸡排冷藏过程中降低了鸡排的氧化和微生物增殖,对小鼠巨噬细胞没有细胞毒性作用。贮藏期间,处理鱼片的pH、干物质、酸度和蒸煮损失值均低于对照。统计分析表明,在贮藏过程中,对照和经LEO处理的玉米的颜色变化非常显著。感官分析发现,添加100 ppm的LEO对鸡肉的感官品质有改善作用。微生物学特征的统计分析明显区分了对照组和LEO处理组(p<0.05)。LEO作为天然食品防腐剂的有效性得到了证明,禽肉产品的保质期延长了约3天。
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引用次数: 0
Evaluation of food safety and health risk factors in food service establishments; a case study in Qom province, Iran 评估食品服务机构的食品安全和健康风险因素;伊朗库姆省的一个案例研究
Pub Date : 2021-11-27 DOI: 10.18502/jfsh.v7i1.7845
Y. Ghafuri, Z. Atafar, G. Jahed Khaniki
Foodborne diseases still occur and affect 30 % of people in the world annually. Existing data related to the status of food safety and health indicators of food services facilities in Qom province indicate that a low percentage of these centers have the necessary criteria in the principles of food safety. According to the factors affecting the promotion of food health and safety indicators, this study was conducted to identify and investigate the factors affecting the risk of food safety in food supply centers in Qom in 2018. In this cross-sectional study, the required data were obtained through validated questionnaires about food safety and environmental health of food services facilities (n=380). Also, the effectiveness of variables such as training on food safety principles through designing before and after study, educational certification, having trading licenses, ownership, urban health status and the age of operators, have been assessed by food safety factors based on relevant tests. The results showed that the training of food handlers in improving and upgrading food safety conditions has had a significant increase. (49 %, mean 5.44 %, SD 3.20 ±, p < 0.001) Also variables such as education level (p < 0.05) and educational certification (p < 0.001) have been effective in increasing food safety and health standards. In order to improve the level of food safety, reviewing the process of educational programs, strengthening educational equipment and media and inter-sectorial coordination to strengthen the role of education should be planned. The food holders, managers of the food establishment and local health experts should enhance the food safety practice.
食源性疾病每年仍会发生并影响到世界上30%的人。与库姆省食品安全状况和食品服务设施健康指标有关的现有数据表明,这些中心中具备食品安全原则中必要标准的比例很低。根据影响食品健康安全指标提升的因素,本研究对2018年库姆市食品供应中心食品安全风险的影响因素进行了识别和调查。在本横断面研究中,通过对食品服务设施食品安全和环境卫生的有效问卷调查(n=380)获得所需数据。通过食品安全因素的相关测试,评估了学习前后通过设计进行食品安全原则培训、教育认证、拥有经营许可证、所有权、城市健康状况、经营者年龄等变量的有效性。结果表明,食品处理人员在改善和提升食品安全条件方面的培训有了显著的增加。(49%,平均值5.44%,标准差3.20±,p < 0.001)此外,教育程度(p < 0.05)和教育认证(p < 0.001)等变量对提高食品安全卫生标准有效。为了提高食品安全水平,应有计划地审查教育计划的过程,加强教育设备和媒体以及跨部门协调,以加强教育作用。食品持有人、食品企业管理者和当地卫生专家应加强食品安全实践。
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引用次数: 1
Isolation and identification of probiotic bacteria from Iranian traditional cheese produced by lamb or kid abomasum microorganisms 伊朗传统奶酪中益生菌的分离与鉴定
Pub Date : 2021-10-20 DOI: 10.18502/jfsh.v6i4.7567
Iraj Hasani, A. Sepahi, S. Shoeibi, Gholamhassan Asadi Aghdash
Different strains of lactic acid bacteria are the most common micro-organisms known as probiotics. Two major kinds of probiotic bacteria are members of the genera Lactobacillus and Bifidobacterium, that are generally associated with the genus Lactobacillus which play a vital role in the body health, as well as the ability to produce antimicrobials and vitamins in the gastrointestinal tract. The purpose of the study was the isolation of probiotics in traditional cheeses that are produced from the natural flora of the newborn mammalian digestive tract. Bacteria from lamb and kid abomasum are added to milk. The curd is slaughtered from the abomasum of a lamb or kid that is suckling and has now no longer began out to devour forage, after which its belly is dried, that is referred to as curd. In this study, sixteen samples of cheeses local to Markazi province of Iran have been prepared. The isolated bacteria have been examined for morphological, biochemical, probiotic properties, and molecular identity. Out of sixteen traditional cheese samples, five kinds of Lactobacillus have been diagnosed through PCR. L. casei strain J026 strain was identified in the traditional cheese samples as the most genera. Using probiotic starters from local sources is a beneficial strategy for producing traditional cheese, which the native strains are more compatible with the humans’ intestinal flora and therefore may also higher play their probiotic’s characteristic.
不同菌株的乳酸菌是最常见的微生物被称为益生菌。两种主要的益生菌是乳酸菌属和双歧杆菌属的成员,它们通常与乳酸菌属有关,乳酸菌属在人体健康中起着至关重要的作用,并且能够在胃肠道中产生抗菌剂和维生素。这项研究的目的是分离传统奶酪中的益生菌,这些奶酪是由新生哺乳动物消化道的天然菌群产生的。从羊肉和山羊胃中提取的细菌被添加到牛奶中。凝乳是从正在吃奶的羊羔或小山羊的皱胃中屠宰出来的,现在已经不再开始吃牧草了,然后把它的肚子晒干,这就是凝乳。在这项研究中,准备了16种伊朗马卡齐省当地的奶酪样品。对分离的细菌进行了形态学、生化、益生菌特性和分子特性的检测。在16份传统奶酪样品中,通过PCR检测出5种乳酸菌。在传统奶酪样品中鉴定出干酪乳杆菌J026属最多。使用本地产的益生菌发酵剂是生产传统奶酪的一种有益策略,因为本地菌株与人体肠道菌群的相容性更强,因此也可以更好地发挥其益生菌的特性。
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引用次数: 0
Rapid determination of histamine levels in canned tuna by a novel spectrophotometric method 新型分光光度法快速测定金枪鱼罐头中组胺含量
Pub Date : 2021-10-20 DOI: 10.18502/jfsh.v6i4.7565
M. Karami, Hadi Eghbaljoo gharehgheshlaghi, Mahmoud Alizadeh-sani, P. Sadighara, Behrouz Tajdar-oranj, N. Shariatifar, E. Molaee-aghaee, Leila Peivasteh-roudsari
Histamine is the most common biogenic amine and is responsible for Scombroid fish poisoning. The presence of histamine at high concentrations in foodstuff indicates a public health issue Therefore, to the assurance of safety and quality, the monitoring of histamine concentration in fish and fishery products is urgent. To this aim, histamine content in 30 samples of canned tuna was measured by the UV-Vis spectrophotometry method. Linear regression was gained (R2 = 0.9905). The range of histamine levels was calculated between 85.04-125.08 mg kg-1 with an overall mean of 98.104 ± 5.18 mg kg-1. 40% of the samples were contained more than 100 mg kg-1, the allowable limit declared by Iranian National Standard (INS). However, The histamine amount of all samples were below the limit set by Codex Alimentarius (200 mg kg-1). There was no significant difference between the mean values of histamine in various brands of canned tuna. This spectrophotometric method used in this study can be introduced as a simple, applicable method for rapid monitoring of fish products, which is based on histamine reaction with copper and Alizarin Red S.
组胺是最常见的生物胺,是造成鲭鱼中毒的原因。食品中存在高浓度组胺是一个公共卫生问题,因此,对鱼类和渔业产品中组胺浓度的监测是保证安全和质量的迫切需要。为此,采用紫外-可见分光光度法测定了30份金枪鱼罐头中组胺的含量。得到线性回归(R2 = 0.9905)。组胺水平范围为85.04 ~ 125.08 mg kg-1,总体平均值为98.104±5.18 mg kg-1。40%的样品含量超过伊朗国家标准(INS)规定的允许限量100mg kg-1。但所有样品的组胺含量均低于国际食品法典委员会规定的200 mg kg-1的限量。不同品牌的金枪鱼罐头中组胺的平均值没有显著差异。本研究采用的基于组胺与铜和茜素红S反应的分光光度法是一种简单、适用的鱼制品快速监测方法。
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引用次数: 0
The antioxidant activity of Lactic acid bacteria and probiotics: a review 乳酸菌和益生菌的抗氧化活性研究进展
Pub Date : 2021-10-20 DOI: 10.18502/jfsh.v6i4.7563
Azita Faraki, F. Rahmani
Probiotics and Lactic Acid Bacteria play important roles such as the production of antimicrobial compounds and other metabolites. So they have positive effects on human health. When reactive oxygen species generated in excess or cellular defenses are deficient, biomolecules can be damaged by the oxidative stress process. Various studies have shown that the best way to protect the human body from the effects of oxidation reactions is to avoid them, which can be accomplished by using antioxidants. Due to the damages of synthetic antioxidants, their usage has been discussed. Nowadays natural antioxidants derived from bio-resources have recently gained a lot of attention as a potential replacement for synthetic antioxidants. Probiotic bacteria are thought to defend against oxidative stress by restoring the gut microbiota, according to hypothesis of some scientists. This type of microorganisms indicated their antioxidant activity by producing and increasing antioxidant enzymes, production of secondary metabolites, small hydrolyzed peptides in food, resistance to the presence of hydrogen peroxide, and production of intracellular and extracellular compounds such as Exopolysaccharides. Also, they have shown their positive effect on in vivo models. In conclusion, according to the results of studies, lactic acid bacteria and probiotics are significant sources of natural antioxidants. Therefore, they have important research value and market development potential. Also, it should be noted that the mechanism of antioxidant activity of this group of microorganisms has not been fully investigated, this requires further research.
益生菌和乳酸菌在产生抗菌化合物和其他代谢产物方面发挥着重要作用。因此,它们对人类健康有积极影响。当过量产生的活性氧或细胞防御不足时,生物分子可能会被氧化应激过程破坏。各种研究表明,保护人体免受氧化反应影响的最佳方法是避免氧化反应,这可以通过使用抗氧化剂来实现。由于合成抗氧化剂的危害,人们讨论了它们的用途。近年来,从生物资源中提取的天然抗氧化剂作为合成抗氧化剂的潜在替代品受到了广泛关注。根据一些科学家的假设,益生菌被认为可以通过恢复肠道微生物群来抵御氧化应激。这类微生物通过产生和增加抗氧化酶、次级代谢产物的产生、食物中小水解肽的产生、对过氧化氢存在的抗性以及胞内和胞外化合物(如外多糖)的产生来表明其抗氧化活性。此外,它们在体内模型上也显示出了积极的作用。总之,根据研究结果,乳酸菌和益生菌是天然抗氧化剂的重要来源。因此,它们具有重要的研究价值和市场发展潜力。此外,需要注意的是,这类微生物的抗氧化活性机制尚未得到充分研究,这需要进一步研究。
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引用次数: 2
Meat quality, biosecurity, and animal welfare: consequences of an inefficient inspection 肉类质量、生物安全和动物福利:低效检验的后果
Pub Date : 2021-10-20 DOI: 10.18502/jfsh.v6i4.7568
P. Tatemoto, Vanessa Louise Santos Oliveira, David Attuy Vey da Silva, Marco Túlio Costa Almeida
In livestock, biosecurity, animal welfare and meat quality have a synergetic effect on the final product. To meet minimal safety and humane production, it is necessary to consider biosecurity risks and procedures to mitigate animal welfare problems. Following guidelines to achieve it, the meat quality improves consequently. Here we tested two parameters regarding the meat quality of beef cattle to understand how the poor inspection affects the quality of the final product. Therefore, 12 samples of the longissimus dorsi muscle were evaluated from different butcheries, using fresh meat recently received by the supplier. There were no sanitary inspection records related to the sampled meat. The results showed a large percentage of PSE (pale, soft, and exudative) and DFD (dark, firm, and dry) meats. Given the pH and color values, it is concluded that the slaughtering was not carried out properly. Hence, technological defects in the meat were observed, making it inappropriate for consumption. We concluded that inefficient pre-slaughter handling is compromising animal welfare, increasing the biosecurity risk, and impairing the quality of the final product. We argue that these problems not only decrease the sustainability of livestock systems, but also compromises human health. Currently, livestock plays an important role in global environmental concerns, such as climate change, land degradation, water pollution and biodiversity loss. Then, it is needed to reduce the waste of such products.
在畜牧业,生物安全、动物福利和肉类质量对最终产品有协同作用。为了实现最低限度的安全和人道生产,有必要考虑生物安全风险和程序,以缓解动物福利问题。按照指导方针来实现这一点,肉的质量也随之提高。在这里,我们测试了关于肉牛肉质的两个参数,以了解不良检查如何影响最终产品的质量。因此,使用供应商最近收到的新鲜肉类,对来自不同肉店的12个背最长肌样本进行了评估。没有与抽样肉类有关的卫生检查记录。结果显示,PSE(苍白、柔软、渗出)和DFD(深色、坚硬、干燥)肉的比例很大。考虑到pH值和颜色值,得出的结论是屠宰没有正确进行。因此,观察到肉的技术缺陷,使其不适合食用。我们得出的结论是,低效的屠宰前处理正在损害动物福利,增加生物安全风险,并损害最终产品的质量。我们认为,这些问题不仅降低了畜牧系统的可持续性,而且损害了人类健康。目前,牲畜在气候变化、土地退化、水污染和生物多样性丧失等全球环境问题中发挥着重要作用。然后,需要减少此类产品的浪费。
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引用次数: 0
Evaluation of heavy metals content in the canned/packed fruit juices from local and imported origin in Lahore, Pakistan 巴基斯坦拉合尔本地和进口果汁罐头/包装中重金属含量的评估
Pub Date : 2021-10-20 DOI: 10.18502/jfsh.v6i4.7564
Nazeefa Fatima, Munazza Khan, Muhammad Shuaib Kabeer
This study was conducted to determine the mean concentration of heavy metals such as lead (Pb), copper (Cu), zinc (Zn), chromium (Cr), manganese (Mn), nickel (Ni), selenium (Se), magnesium (Mg), and iron (Fe) in canned/packed fruits juices, collected from various stores in Lahore in a period of three months. These juices were categorized into four groups; local packed and canned and also imported packed and canned products. Every group consisted of ten samples. By using the di-acid digestion method, the collected samples were digested and analyzed under Atomic Absorption Spectrophotometer (AAS). The results indicated that the mean values of 7 out of 9 tested heavy metals including Pb, Mg, Ni, Fe, Cr, Se and Mn were above permissible limits (set by WHO) in all four understudy groups. Therefore, it was concluded that commercially available fruit juices are not all safe according to their heavy metals content for the human consumption despite their nutritive values.
本研究旨在测定三个月内从拉合尔各商店收集的罐装/包装果汁中铅(Pb)、铜(Cu)、锌(Zn)、铬(Cr)、锰(Mn)、镍(Ni)、硒(Se)、镁(Mg)和铁(Fe)等重金属的平均浓度。这些果汁被分为四组:;本地包装和罐装以及进口包装和罐装产品。每组由10个样本组成。采用二酸消解法,在原子吸收分光光度计(AAS)下对采集的样品进行消解和分析。结果表明,在所有四个替代研究组中,9种测试重金属中有7种(包括Pb、Mg、Ni、Fe、Cr、Se和Mn)的平均值均高于世界卫生组织规定的允许限值。因此,得出的结论是,尽管有营养价值,但根据其重金属含量,市售果汁对人类消费并不完全安全。
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引用次数: 0
The role and importance of food industry and food systems during the COVID-19 and the possibility of cold plasma technology for inactivation: a review 食品工业和食品系统在COVID-19期间的作用和重要性以及冷等离子体灭活技术的可能性:综述
Pub Date : 2021-08-10 DOI: 10.18502/jfsh.v6i3.6942
Mahsa Jahangiri Siahmazgi, Y. Ramezan
This article reviews the role and importance of food systems during the COVID-19 outbreak, the impact of this pandemic on the food industry and chain, and examining the methods and possibilities of using cold plasma technology to deal with this pandemic. Since food plays an essential role in human health as an integral part of human life, food safety is critical in such cases. It is essential to adopt practical strategies in controlling the COVID-19 crisis. In the current situation, several methods are used to disinfect and control the spread of disease, some of which are ineffective and can also have problems and limitations. Therefore, emerging technologies such as cold plasma (CP) technology can help facilitate the control process and reduce pandemics. The capabilities of this method are disinfection of food, equipment, production surfaces, and indoor with poorly ventilated spaces of SARS‐CoV‐2 through aerosol microdroplets, which can remain in the air for a long time; such as public transportation, production lines, and restaurants where the possibility of transmitting the SARS‐CoV‐2 is high. On the one hand, this review summarizes all available data related to cold plasma technology as an emerging technology to inactivate SARS‐CoV‐2. It then gives us a brief explanation of the role and importance of the food industry and food supply chain during the pandemic.
本文回顾了食品系统在新冠肺炎爆发期间的作用和重要性,以及这一流行病对食品行业和供应链的影响,并研究了使用冷等离子体技术应对这一流行病的方法和可能性。由于食品作为人类生活的一个组成部分,在人类健康中发挥着重要作用,因此在这种情况下,食品安全至关重要。采取切实可行的战略控制新冠肺炎危机至关重要。在目前的情况下,有几种方法被用来消毒和控制疾病的传播,其中一些方法无效,也可能存在问题和局限性。因此,冷等离子体(CP)技术等新兴技术有助于促进控制过程并减少流行病。这种方法的能力是通过气溶胶微滴对食物、设备、生产表面和通风不良的室内严重急性呼吸系统综合征冠状病毒2型进行消毒,气溶胶微滴可以在空气中长期存在;如公共交通、生产线和餐馆,传播严重急性呼吸系统综合征冠状病毒2型的可能性很高。一方面,这篇综述总结了与冷等离子体技术相关的所有可用数据,冷等离子体技术是一种灭活SARS‐CoV‐2的新兴技术。然后,它向我们简要解释了食品行业和食品供应链在疫情期间的作用和重要性。
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引用次数: 0
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Journal of food safety and hygiene
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