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Impact of drying method as pretreatment for extraction of bioactive compounds from jambolan (Syzygium cumini (L.) Skeels) 干燥法预处理对大头菜生物活性物质提取的影响斯基尔)
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.05521
A. E. Santos, G. P. Aguiar, Camila Dal Magro, Roberto Alves Lacowicz, Isabela Maia Toaldo Fedrigo, M. Bordignon-Luiz, J. V. Oliveira, M. Lanza
Abstract Jambolan (Syzygium cumini (L.) Skeels) is an under-explored fruit rich in polyphenols, which are associated with health benefits, such as increasing resistance to oxidative stress, inflammatory processes and cardiovascular, and platelet functions. These polyphenols can be obtained by extraction, but an efficient standard method remains a challenge. In this context, this work evaluated the impact of different pretreatments on jambolans to obtain bioactive compounds by aqueous extraction. An Air Circulation Oven (ACO) and Lyophilization (LYO) were used as pretreatments. In addition, the influence of mass, temperature, cycle, and time parameters were studied in the extraction methods used: Percolated Solid-Liquid (PSL), Conventional Solid-Liquid (CSL), and solid-liquid assisted by ultrasound (USL). The extraction yield was from 7.3% (ACO) to 46.3% (LYO), both using the PSL method. In addition, eleven phenolic compounds and six anthocyanins were detected by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), in expressive amounts of catechin and cyaniding in the LYO sample and, these extracts showed higher concentrations of bioactive compounds. The CSL method was more efficient on ACO samples and PSL on LYO samples. LYO extracts showed higher concentrations of bioactive compounds. Therefore, the use of a drying pretreatment results in extracts with a high antioxidant potential for application in the food, cosmetic, and pharmaceutical markets.
Jambolan (Syzygium cumini (L.))果皮是一种富含多酚的未开发水果,多酚对健康有益,如增强对氧化应激、炎症过程和心血管以及血小板功能的抵抗力。这些多酚可以通过萃取得到,但有效的标准方法仍然是一个挑战。在此背景下,本工作评估了不同的预处理方法对竹柱水萃取获得生物活性化合物的影响。采用空气循环烘箱(ACO)和冻干(LYO)作为预处理。此外,还研究了质量、温度、周期、时间等参数对渗透固液萃取法(PSL)、常规固液萃取法(CSL)和超声辅助固液萃取法(USL)的影响。两种方法的提取率均为7.3% (ACO) ~ 46.3% (LYO)。此外,通过高效液相色谱-二极管阵列检测器(HPLC-DAD)检测到11种酚类化合物和6种花青素,在LYO样品中表达了大量的儿茶素和氰化,这些提取物显示出较高的生物活性化合物浓度。CSL法在ACO样品上效率更高,PSL法在LYO样品上效率更高。LYO提取物显示出较高的生物活性成分浓度。因此,使用干燥预处理可以得到具有高抗氧化潜力的提取物,可用于食品、化妆品和制药市场。
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引用次数: 0
Effect of pre-gelatinized temperature on physical and nutritional content of indonesian instant cassava leaves porridge: rowe luwa 预糊化温度对印尼即食木薯叶粥rowe luwa物理和营养成分的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.05021
A. Iwansyah, Trian Apriadi, Dede Zainal Arif, Y. Andriana, A. Indriati, N. I. Mayasti, R. Luthfiyanti
Abstract Rowe luwa is a traditional porridge from Southwest Sumba, Indonesia, made from the steamed pulp cassava leaves pounded together with rice. This study examined the effect of pre-gelatinization temperature on the physical quality and nutritional content of instant rowe luwa porridge. The experimental design used in this study was a Completely Randomized Design (CRD) with pre-gelatinization temperature factors, viz., 60 °C (X1), 65 °C (X2), 70 °C (X3), 75 °C (X4) and 80 °C (X5). Physical properties were measured by analyzing color, viscosity, rehydration, and syneresis, while for nutritional compositions, water content, ash, fat, protein, carbohydrate, and energy were evaluated. The simple linear regression was employed to examine the correlation between pre-gelatinization temperature and physical properties or nutritional composition. The results showed that the pre-gelatinization temperature correlated with physical properties, such as: viscosity (r = 0.9924), rehydration (r = 0.807) and syneresis (r = 0.841). Furthermore, the pre-gelatinized temperature significantly affected protein and carbohydrate contents (p < 0.05), while the moisture, ash, and fat contents showed negligible effect (p > 0.05). Principal Component Analysis (PCA) showed that instant rowe luwa porridge prepared with the pre-gelatinization temperature of 80 °C (object X5) had the highest viscosity, rehydration, syneresis, and protein contents compared to the other cooking temperatures. These preliminary data are useful for further research to determine the method and optimization formula of the instant rowe luwa porridge.
罗维卢瓦是印度尼西亚松巴西南部的一种传统粥,由蒸熟的木薯叶浆与大米一起捣碎而成。本研究考察了预糊化温度对即食罗泥粥物理品质和营养成分的影响。本研究采用完全随机设计(CRD),预糊化温度因子分别为60℃(X1)、65℃(X2)、70℃(X3)、75℃(X4)和80℃(X5)。物理性质通过分析颜色、粘度、再水合作用和协同作用来测量,而营养成分则对含水量、灰分、脂肪、蛋白质、碳水化合物和能量进行了评估。采用简单线性回归法考察预糊化温度与物理性质或营养成分的相关性。结果表明,预糊化温度与粘度(r = 0.9924)、复水化(r = 0.807)、协同作用(r = 0.841)等物理性能相关。预糊化温度对蛋白质和碳水化合物含量影响显著(p < 0.05),对水分、灰分和脂肪含量影响不显著(p < 0.05)。主成分分析(PCA)结果表明,预糊化温度为80℃(对象X5)时制备的即食罗洼粥黏度、复水、协同作用和蛋白质含量均高于其他温度。这些初步数据为进一步研究即食罗泥粥的制备方法和优化配方提供了依据。
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引用次数: 1
Consumer-consumption characteristics of ready-to-eat sous vide food products within the habitual context of the household 消费者在家庭习惯背景下对即食真空烹调食品的消费特征
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.05121
M. Aviles, E. Naef, M. B. Gómez, R. A. Abalos
Abstract Preparing cooked food by sous vide is an alternative for the design of products that permit the consumer to eat quickly and practically, without losing the sensory and nutritional characteristics of foods. This study aimed to determine the sensory properties, the overall liking, and the consumer-consumption characteristics and predispositions regarding ready-to-eat high-nutritional-quality preparations made with locally produced vegetables and cooked by the sous vide technique. A vegetable millefeuille and a chicken and vegetable hamburger were prepared for sensory characterization. Questions related to the consumer's consumption characteristics and predisposition to purchase this type of product were incorporated into the survey. Consumers performed the sensory evaluation in their home, within the habitual living ambience where they consumed their usual diet. The results revealed that most consumers accepted both millefeuille (100 %) and chicken and vegetable hamburger (92 %) and also reported a predisposition for purchasing both preparations (87 % and 84 %, respectively). The main characteristics that made the consumption attractive were quality (95%), market availability (89 %), lack of practical cooking skills (87 %), the price (84 %), the shortness of time invested in preparation (78 %), and the consumer's occupation (73 %). These types of preparations constitute an innovative alternative for the design of products of higher nutritional quality, without ignoring the requirements for their consumption.
摘要:通过真空烹调技术制备熟食是一种设计产品的替代方案,它可以让消费者快速而实际地进食,而不会失去食物的感官和营养特性。本研究的目的是确定感官特性,整体喜好,以及消费者的消费特征和倾向对即食高营养品质的准备与本地生产的蔬菜和真空烹调技术。为了进行感官表征,我们准备了蔬菜千面和鸡肉蔬菜汉堡。与消费者的消费特征和购买这类产品的倾向相关的问题被纳入调查。消费者在家中进行感官评估,在他们习惯的生活环境中,他们吃他们通常的饮食。结果显示,大多数消费者都接受millefeuille(100%)和鸡肉蔬菜汉堡(92%),并报告了购买这两种制剂的倾向(分别为87%和84%)。使消费具有吸引力的主要特征是质量(95%),市场可用性(89%),缺乏实用的烹饪技能(87%),价格(84%),准备时间短(78%)和消费者的职业(73%)。这些类型的制剂在不忽视其消费要求的情况下,构成了设计更高营养质量产品的创新选择。
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引用次数: 0
Perfil do consumidor de alimentos funcionais: identidade e hábitos de vida 功能食品消费者概况:身份与生活习惯
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.07221
Giovana Flores Safraid, Cristine Zanquetta Portes, Roseana Machado Dantas, Â. G. Batista
Resumo Com a mudança na alimentação dos adultos nas últimas décadas, aumenta o desenvolvimento de doenças crônicas não transmissíveis. Aumentar o consumo de alimentos funcionais, com ações metabólicas benéficas ao organismo, é uma estratégia para proporcionar benefícios à saúde em longo prazo. O objetivo deste trabalho foi identificar os fatores que têm influência na percepção e no consumo de alimentos funcionais por adultos. Neste estudo, dentre os fatores que interferem no consumo de alimentos funcionais identificados, os aspectos socioeconômicos, de saúde e de qualidade dos alimentos se destacaram. No âmbito geral, apesar de controvérsias, os estudos caracterizam os consumidores de alimentos funcionais como mulheres, de boa situação financeira e alto nível de escolaridade, que se preocupam com a saúde de forma global, na medida em que praticam atividades físicas e têm uma alimentação adequada. Ademais, percebe-se que a cultura local tem bastante influência na percepção dos alimentos funcionais. Alguns fatores, como a familiaridade, sejam do produto, seja da alegação funcional, também são importantes para determinar a atitude do consumidor perante esses alimentos. O sabor é o principal atributo sensorial que define o consumo de alimentos funcionais. Os consumidores também tendem a preferir alimentos que são considerados naturais e não processados. Portanto, estudos que relacionem hábitos globais de saúde e o consumo de alimentos funcionais podem ser interessantes para assim nortear as intervenções e a inovação na produção de alimentos nesta área.
摘要近几十年来,随着成人饮食的改变,慢性非传染性疾病的发展增加。增加对身体有益代谢作用的功能性食品的消费是提供长期健康益处的一种策略。本研究的目的是确定影响成人功能性食品感知和消费的因素。在本研究中,在影响功能性食品消费的因素中,社会经济、健康和食品质量方面脱颖而出。总的来说,尽管存在争议,但研究将功能性食品的消费者描述为经济状况良好、受教育程度高的妇女,她们关心全球健康,因为她们从事体育活动并有足够的营养。此外,当地文化对功能性食品的感知有很大的影响。一些因素,如对产品或功能声明的熟悉程度,在决定消费者对这些食品的态度方面也很重要。风味是定义功能性食品消费的主要感官属性。消费者也倾向于选择被认为是天然和未经加工的食品。因此,将全球健康习惯与功能性食品消费联系起来的研究可能有助于指导这一领域食品生产的干预和创新。
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引用次数: 1
Green banana and ora-pro-nóbis mixed flours: nutritional and technological characteristics 青香蕉ora-pro-nóbis混合粉:营养与工艺特性
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.08122
Hebert Teixeira Cândido, M. Leonel, S. Leonel, Lucas Felipe dos Ouros, Paulo Ricardo Rodrigues de Jesus, Maiqui Izidoro, Nicholas Zanette Molha, Vinícius Martins Domiciano
Abstract The constant need for innovation in the food industries to meet the demands of consumers and the growing market for gluten-free and high-protein products have driven studies of mixed flour. In this study, 'BRS Platina' banana fruits were processed to obtain Whole Green Banana Flour (WGBF) and Green Banana Pulp Flour (GBPF) as well as leaves of Ora-pro-nóbis Flour (OPNF). Mixed flours were prepared by blending banana flours (WGBF or GBPF) with increasing levels of OPNF (from 5 to 25%). Flours were analyzed for physicochemical characteristics, mineral contents, Water Absorption Index (WAI), Water Solubility Index (WSI), and color. The main component of banana flours was starch, in which GBPF has the highest content. The WGBF had a higher mineral content, and in both flours, the potassium was at a greater level. OPNF showed the highest protein and mineral contents. Independent of the type of banana flour, mixed flours were darker, and had higher WAI and considerable increases in Mg, Fe, and Zn than no mixed ones. The blend of banana flours and Ora-pro-nóbis leaf flour can be a viable combination for preparing a variety of food products, due to its nutritional and technological properties.
食品工业不断创新,以满足消费者的需求,以及市场对无麸质和高蛋白产品的需求不断增长,这推动了混合面粉的研究。本研究以“BRS Platina”香蕉果实为原料,加工得到全青香蕉粉(WGBF)和青香蕉浆粉(GBPF)以及Ora-pro-nóbis粉(OPNF)叶。将香蕉粉(WGBF或GBPF)与OPNF水平增加(从5%到25%)混合制备混合面粉。分析了面粉的理化特性、矿物质含量、吸水指数(WAI)、水溶性指数(WSI)和颜色。香蕉粉的主要成分是淀粉,其中GBPF含量最高。WGBF的矿物质含量较高,两种面粉中钾含量均较高。OPNF的蛋白质和矿物质含量最高。与香蕉粉的种类无关,混合面粉的颜色更深,WAI更高,Mg、Fe和Zn的含量也明显增加。香蕉粉和Ora-pro-nóbis叶粉的混合物由于其营养和技术特性,可以成为制备各种食品的可行组合。
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引用次数: 1
Evaluating ultrasound pre-treatment as a tool for improving the process of a fermented beverage made from pineapple by-products 评价超声预处理作为改进菠萝副产物发酵饮料工艺的工具
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.11621
Karla Aguilar
Abstract Fermentation has the potential of converting fruit by-products into value-added products via an efficient, sustainable, and low-cost process. Traditionally, Mexicans use pineapple residues to produce a fermented beverage called tepache. As this soft drink is increasingly consumed in restaurants, it is necessary to develop an effective and reliable process to yield a final product with desirable physicochemical properties. In this work, tepache was prepared using an ultrasound pre-treatment to enhance the fermentation process and improve the end-product quality. The ultrasound was provided by a probe (25 kHz, 400 W) submerged in pineapple preparations before fermentation. Characterization of physicochemical properties was performed on samples processed under different types of amplitude (20 and 100%) and sonication time (5 and 10 min). In all samples, the pH, acidity, and ºBrix values were similar to those in commercial tepaches. On the other hand, microscopy revealed that 5 min of sonication induced positive changes in the suspended matter responsible for the physical stability of fruit beverages. The tepaches obtained with this method had color uniformity. Indeed, 5 min of sonication at the highest amplitude (16.34 kJ・cm-2) augmented the soluble solids during the initial phases of fermentation. Moreover, the results from IR spectroscopy proved that ultrasound helped the ethanol release from yeasts. The maximum ethanol yield, calculated by model fitting, had a positive variation of 35%. These findings prove that ultrasound is capable to induce physicochemical changes useful for the industrial production of tepache.
发酵具有通过高效、可持续和低成本的过程将水果副产品转化为增值产品的潜力。传统上,墨西哥人用菠萝渣制作一种叫做tepache的发酵饮料。随着这种软饮料越来越多地在餐馆消费,有必要开发一种有效和可靠的工艺,以产生具有理想物理化学性质的最终产品。在本研究中,采用超声预处理法制备tepache,以增强发酵过程,提高最终产品质量。在发酵前,用探针(25 kHz, 400 W)浸泡在菠萝制剂中。对不同振幅(20%和100%)和超声时间(5和10 min)下处理的样品进行了理化性质表征。在所有样品中,pH值、酸度和白锐度值与商业样品相似。另一方面,显微镜显示,5分钟的超声诱导悬浮物的积极变化,负责水果饮料的物理稳定性。该方法获得的膜片具有颜色均匀性。事实上,在发酵的初始阶段,以最高振幅(16.34 kJ·cm-2)进行5分钟的超声处理可以增加可溶性固形物。红外光谱分析结果表明,超声波有助于酵母中乙醇的释放。通过模型拟合计算得到的最大乙醇产率为35%。这些发现证明了超声波能够诱导对乙酰胆碱工业生产有用的物理化学变化。
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引用次数: 3
Desenvolvimento e caracterização de sorvete funcional de alto teor proteico com ora-pro-nóbis (Pereskia aculeata Miller) e inulina 含ora-pro- nobis (Pereskia aculeata Miller)和菊粉的功能性高蛋白冰淇淋的开发与表征
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.12920
Pedro Paullo Alves dos Santos, Gabrieli da Silva Ferrari, Márcia Silva Rosa, K. Almeida, Lucas de Andrade de Araújo, Maíra de Carvalho Pereira, Maria Eulália Felix Wanderley, Priscila Neder Morato
Resumo A utilização de matérias-primas funcionais vem crescendo nos últimos anos e tem como objetivo fornecer, além de nutrientes essenciais, compostos associados a efeitos benéficos à saúde. Dentre os ingredientes com alegações funcionais, a inulina tem sido considerada uma importante fibra alimentar. Além disso, o conhecimento e a utilização de Plantas Alimentícias Não Convencionais (PANCs) vêm recebendo destaque, valorizando a biodiversidade e a alimentação mais sustentável. Assim, o presente estudo teve como objetivo desenvolver sorvetes utilizando matérias-primas não convencionais, a planta ora-pro-nóbis e a inulina, a fim de produzir alimento funcional e inovador. Foram elaboradas duas formulações de sorvetes, que se diferenciaram apenas pela quantidade de inulina. Na Formulação 1 (F1), o sorvete de ora-pro-nóbis recebeu adição de 3% de inulina e, na Formulação 2 (F2), o sorvete de ora-pro-nóbis recebeu adição de 6% de inulina. Foram realizadas análises físico-químicas e de aminoácidos das folhas de ora-pro-nóbis. Posteriormente, foram conduzidas as análises microbiológicas, físico-químicas e sensoriais das formulações de sorvete. Os resultados foram comparados estatisticamente pelo método t-Student com nível de significância de (p˂0,05). O teor proteico das formulações foi elevado, 10,54 ± 2,20% para F1 e 12,13 ± 0,57% para F2. Não houve diferença significativa nos conteúdos de lipídios, cinzas, proteínas e fibra bruta entre as formulações. As amostras estão de acordo com os padrões microbiológicos estabelecidos pela legislação. Com relação à análise de incorporação de ar, a F2 apresentou valor superior (41,26 ± 0,13%) comparada à F1 (36,36 ± 0,17%). Além disso, a F2 apresentou menor taxa de derretimento (1,20 g/minuto) que a F1 (3,38 g/minuto). Os sorvetes apresentaram boa aceitação sensorial e podem servir como opção de consumo para as pessoas que procuram um produto saudável e com elevado teor de proteínas.
摘要近年来,功能性原料的使用越来越多,其目的是提供与健康有益作用相关的必需营养素和化合物。在声称具有功能的成分中,菊粉被认为是一种重要的膳食纤维。此外,非传统食物植物的知识和使用受到重视,重视生物多样性和更可持续的食物。因此,本研究的目的是利用非传统原料、植物和菊粉开发冰淇淋,以生产功能性和创新性的食品。研制了两种冰淇淋配方,其区别仅在于菊粉的含量。配方1 (F1)中添加3%菊粉,配方2 (F2)中添加6%菊粉。对其叶片进行了理化分析和氨基酸分析。随后,对冰淇淋配方进行了微生物学、理化和感官分析。结果采用t-Student方法进行统计学比较,显著性水平为(p < 0.05)。配方蛋白质含量较高,F1为10.54±2.20%,F2为12.13±0.57%。各配方的脂质、灰分、蛋白质和粗纤维含量无显著差异。样品符合法律规定的微生物标准。在空气掺入分析中,F2的值(41.26±0.13%)高于F1(36.36±0.17%)。此外,F2的熔化速率(1.20 g/min)低于F1 (3.38 g/min)。冰淇淋具有良好的感官接受度,可以作为寻求健康和高蛋白产品的人的消费选择。
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引用次数: 2
Optimization of enzymatic hydrolysis of Pleurotus ostreatus derived proteins through RSM and evaluation of nutritional and functional qualities of mushroom protein hydrolysates RSM法优化平菇衍生蛋白酶解工艺及蛋白水解产物的营养和功能品质评价
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.18620
B. Goswami, S. Majumdar, R. Dutta, J. Bhowal
Abstract Pleurotus ostreatus (Jacq.) P. Kumm., the second most widely cultivated oyster mushroom was grown on paddy straw, which is cheap and readily available waste material. After harvesting and drying, nutritional, and antinutritional composition of P. ostreatus were estimated using the standard assay methods. Tannin and phytic acid were present in very negligible amount (0.095 ± 0.027 mg/g and 0.150 ± 0.083 mg/g, respectively), whereas oxalate and cyanide were absent in whole mushroom. In fact, P. ostreatus was hydrolysed with commercially available proteinase K, pepsin and trypsin with different concentrations of the enzymes (0.05%, 0.10% and 0.15%), at different temperatures (30 °C, 40 °C and 50 °C) for different time periods (60, 90 and 120 min) to get the mushroom protein hydrolysates. Degree of hydrolysis and protein content varied from 4.29 ± 1.12% to 99.42 ± 0.02% and from 0.25 ± 0.07 mg/mL to 3.22 ± 0.12 mg/mL, respectively. Maximum degree of hydrolysis and the highest protein content of protein hydrolysate was obtained when using 0.15% proteinase K, at 50 °C for 120 minutes. Mushroom protein hydrolysates thus obtained exhibited improved functional characteristics such as foaming capacity, foaming stability and emulsifying property than the unhydrolysed mushroom. Based on the result of the present study, the mushroom protein hydrolysates could be served as useful ingredient for food and nutraceutical applications.
摘要:平菇(Pleurotus ostreatus)p . Kumm。在美国,种植面积第二大的平菇是在稻秆上种植的,稻秆是一种廉价且易得的废料。在采收和干燥后,用标准的测定方法估计了白藜芦醇的营养和抗营养成分。单宁和植酸含量非常低(分别为0.095±0.027 mg/g和0.150±0.083 mg/g),而草酸和氰化物则不存在。事实上,在不同温度(30°C、40°C和50°C)、不同时间(60、90和120 min)下,用市购的蛋白酶K、胃蛋白酶和胰蛋白酶分别用不同浓度的酶(0.05%、0.10%和0.15%)对P. ostreatus进行水解,得到蘑菇蛋白水解产物。水解度为4.29±1.12% ~ 99.42±0.02%,蛋白质含量为0.25±0.07 mg/mL ~ 3.22±0.12 mg/mL。当使用0.15%的蛋白酶K,在50°C, 120分钟时,水解程度最大,蛋白水解产物的蛋白含量最高。由此得到的蘑菇蛋白水解产物比未水解的蘑菇具有更好的功能特性,如发泡能力、发泡稳定性和乳化性能。根据本研究结果,蘑菇蛋白水解物可作为食品和营养保健品的有益成分。
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引用次数: 4
Impact of controlled fermentation on the volatile aroma of roasted cocoa 控制发酵对烘焙可可挥发性香气的影响
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.27020
Y. P. Rahardjo, Khaswar Syamsu, Sapta Rahardja, Samsudin, D. Mangunwijaya
Abstract The study of controlled methods of cocoa fermentation on a small scale is important to assess the maintenance of heat generated in the last days of fermentation. The research aimed to study the impact of spontaneous fermentation in controlled fermentation systems on the quality and acceptability of fermented cocoa beans. A 2×3 complete factorial design used different controlled fermentation systems (jacket system, solar heater and wooden box) and pulp reduction as variables. Samples were analyzed for fermentation index and volatile aroma composition profile using Headspace-Solid Phase Microextraction (HS-SPME) and Gas Chromatography-Mass Spectrometry (GC-MS). The profile of volatile compounds is evaluated for the studied variables using a multivariate Principal Components Analysis (PCA). The results showed increasing fermentation times in the jacket system seeing that it raised the fermentation rate and accelerated it to five days of fermentation combined with pulp reduction. The PCA analysis showed differences in the chemical composition of volatile compounds that were mainly associated with the reduction of the pulping process than the type of controlled system in four days of fermentation.
研究可可发酵的小尺度控制方法对于评估发酵最后几天产生的热量的维持是重要的。本研究旨在研究受控发酵系统中自发发酵对发酵可可豆质量和可接受性的影响。2×3全因子设计使用不同的控制发酵系统(夹套系统,太阳能加热器和木箱)和纸浆还原作为变量。采用顶空-固相微萃取(HS-SPME)和气相色谱-质谱(GC-MS)分析样品的发酵指数和挥发性香气成分。利用多元主成分分析(PCA)对研究变量的挥发性化合物进行了评估。结果表明,夹套系统的发酵次数增加,发酵速率提高,发酵时间缩短至5天,并伴有纸浆还原。主成分分析表明,在4天的发酵过程中,挥发性化合物的化学成分的差异主要与制浆过程的减少有关,而不是与控制系统的类型有关。
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引用次数: 3
Polyphenols, carotenoids and flavonoids in an antioxidant probiotic yogurt made with tumbo pulp (Passiflora tripartita Kunth) 含多酚、类胡萝卜素和黄酮类化合物的抗氧化益生菌酸奶
Q3 Agricultural and Biological Sciences Pub Date : 2022-01-01 DOI: 10.1590/1981-6723.17521
Miguel Ángel Inocente-Camones, Gladys Constanza Arias-Arroyo, Saby Mauricio-Alza, G. Bravo-Araujo, Marleny Flor Capcha-Siccha, Emely Cabanillas-Alvitrez
Abstract The species Passiflora tripartita Kunth (tumbo) is endemic to South America, whose edible fruits are a rich source of antioxidant metabolites. This study aimed to develop a probiotic yogurt with tumbo fruit pulp, consisting of an adequate antioxidant capacity related to its content in phenolic compounds, flavonoids, and carotenoids, and with good acceptability. Antioxidant capacity was determined by radical scavenging capacity test (DPPH●) and cation radical scavenging capacity test (ABTS+●), total phenol content by Folin Ciocalteu method, total flavonoids, and carotenoids by spectrophotometric method, on days 1, 7, 14 and 21 of storage. The surface plate count method quantified Lactic Acid Bacteria (LAB). The results evidenced that at day 21 of the analysis, the antioxidant capacity presented high values (DPPH●: 8.774 mg GAE/g, 3.386 mg TAE/g, 6.159 mg AAE/g; ABTS+●: 11.630 mg GAE/g, 7.018 mg TAE/g, 9.218 mg AAE/g), the content of phenolic compounds presented high values (3746.389 mg TPGAE/g; 2355.933 mg TPTAE/g), as well as total flavonoids (52.421 mg Quercetin/g) and carotenoids (72.109 µg β-carotene/g). Yogurt presents a value of 3.4 x 108 CFU/g of LAB and it is therefore considered a probiotic. High values were determined as the sensory characteristics such as odor (6.89), color (6.97), texture (6.94), flavor (6.97), and acceptability (6.94), thus being analyzed according to the hedonic scale in 200 panelists. The physicochemical and microbiological quality of the yogurt complies with current regulations. In conclusion, the probiotic yogurt developed with tumbo fruit pulp had a high amount of LAB. It presented high antioxidant capacity correlated with its high content of phenolic compounds, flavonoids and carotenoids, which remained high during the 21 days of storage. Furthermore, it showed high acceptability and had adequate physicochemical and microbiological quality.
摘要西番莲(Passiflora tripartita Kunth)是南美洲特有的植物,其可食用的果实是抗氧化代谢产物的丰富来源。本研究旨在开发一种具有抗氧化能力的益生菌酸奶,该酸奶含有丰富的酚类化合物、类黄酮和类胡萝卜素,具有良好的可接受性。在贮藏第1、7、14、21天,采用自由基清除能力试验(DPPH●)和阳离子自由基清除能力试验(ABTS+●)测定抗氧化能力,用Folin Ciocalteu法测定总酚含量,分光光度法测定总黄酮含量和类胡萝卜素含量。平板计数法定量乳酸菌(LAB)。结果表明,在分析第21天,抗氧化能力较高(DPPH: 8.774 mg GAE/g, 3.386 mg TAE/g, 6.159 mg AAE/g;ABTS+●:11.630 mg GAE/g, 7.018 mg TAE/g, 9.218 mg AAE/g),其中酚类化合物含量较高(3746.389 mg TPGAE/g;2355.933 mg TPTAE/g),总黄酮(52.421 mg槲皮素/g)和类胡萝卜素(72.109µg β-胡萝卜素/g)。酸奶的乳酸值为3.4 × 108 CFU/g,因此被认为是一种益生菌。高值被确定为气味(6.89)、颜色(6.97)、质地(6.94)、味道(6.97)和可接受性(6.94)等感官特征,从而根据200名小组成员的享乐量表进行分析。酸奶的理化和微生物质量符合现行规定。综上所述,利用汤姆博果肉制作的益生菌酸奶具有较高的乳酸菌含量。其抗氧化能力与其酚类化合物、类黄酮和类胡萝卜素的高含量有关,在21 d的贮藏期间保持较高的含量。此外,它具有较高的可接受性和足够的理化和微生物质量。
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引用次数: 3
期刊
Brazilian Journal of Food Technology
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