Pub Date : 2024-03-02DOI: 10.1007/s40415-024-00992-2
Otávio Miranda Verly, Antonio Miguel Olivo-Neto, Carlos Alberto Ramos Domiciano, Indira Bifano Comini, Simone Silva, Carlos Moreira Miquelino Eleto Torres, Maria Paula Miranda Xavier Rufino, Laiz de Oliveira Sartori, Reginaldo Antonio Medeiros
Alien species pose a potential threat to biodiversity, necessitating vigilant monitoring to inform effective control strategies and prevent their invasiveness. The primary objective of this study was to evaluate the impact of alien species on the richness, horizontal structure, and diversity of the tree–shrub layer within a Cerrado-Pantanal transition region fragment. A floristic census was conducted across a fragment of approximately 3.0 ha, measuring all individuals with an aboveground diameter (DAG) ≥ 5.0 cm. The study assessed the statistical significance of diversity variation in the presence and absence of alien species using the Hutcheson t-test and examined the correlation between the proportion of alien species and various community parameters through Pearson’s correlation. The findings revealed a total of 84 species belonging to 34 families, with a total of 2218 recorded individuals. Notably, eight species (9.52%) were identified as alien, distributed across five families. Among the alien species, Leucaena leucocephala (Lam.) de Wit exhibited the highest importance value. However, the analysis indicated that the presence of alien species did not yield a significant variation in diversity, as demonstrated by the applied test with a 5.0% probability level, at both the plot and community levels. Moreover, the correlation analysis indicated that the community variables exhibited a low association with the proportion of individuals belonging to alien species. Specifically, the correlation decreased in the following order: Shannon–Weaver Diversity Index, density of native species, basal area of native species, and richness of native species. The observed low correlation coefficients (r) and nonsignificant p-values (p < 0.05) suggested that the presence of alien species did not exert a significant influence on the richness, structure, and diversity of the tree–shrub layer within the studied fragment. This outcome was likely facilitated by the absence of dominant populations of alien species. Thus, the study concludes that, at least within this specific context, alien species do not exert a significant influence on the assessed community parameters.
{"title":"Are the anthropized Cerradão of the Cerrado-Pantanal ecotone resistant to alien species invasion?","authors":"Otávio Miranda Verly, Antonio Miguel Olivo-Neto, Carlos Alberto Ramos Domiciano, Indira Bifano Comini, Simone Silva, Carlos Moreira Miquelino Eleto Torres, Maria Paula Miranda Xavier Rufino, Laiz de Oliveira Sartori, Reginaldo Antonio Medeiros","doi":"10.1007/s40415-024-00992-2","DOIUrl":"https://doi.org/10.1007/s40415-024-00992-2","url":null,"abstract":"<p>Alien species pose a potential threat to biodiversity, necessitating vigilant monitoring to inform effective control strategies and prevent their invasiveness. The primary objective of this study was to evaluate the impact of alien species on the richness, horizontal structure, and diversity of the tree–shrub layer within a <i>Cerrado-Pantanal</i> transition region fragment. A floristic census was conducted across a fragment of approximately 3.0 ha, measuring all individuals with an aboveground diameter (DAG) ≥ 5.0 cm. The study assessed the statistical significance of diversity variation in the presence and absence of alien species using the Hutcheson t-test and examined the correlation between the proportion of alien species and various community parameters through Pearson’s correlation. The findings revealed a total of 84 species belonging to 34 families, with a total of 2218 recorded individuals. Notably, eight species (9.52%) were identified as alien, distributed across five families. Among the alien species, <i>Leucaena leucocephala</i> (Lam.) de Wit exhibited the highest importance value. However, the analysis indicated that the presence of alien species did not yield a significant variation in diversity, as demonstrated by the applied test with a 5.0% probability level, at both the plot and community levels. Moreover, the correlation analysis indicated that the community variables exhibited a low association with the proportion of individuals belonging to alien species. Specifically, the correlation decreased in the following order: Shannon–Weaver Diversity Index, density of native species, basal area of native species, and richness of native species. The observed low correlation coefficients (r) and nonsignificant p-values (<i>p</i> < 0.05) suggested that the presence of alien species did not exert a significant influence on the richness, structure, and diversity of the tree–shrub layer within the studied fragment. This outcome was likely facilitated by the absence of dominant populations of alien species. Thus, the study concludes that, at least within this specific context, alien species do not exert a significant influence on the assessed community parameters.</p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140017102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-27DOI: 10.1007/s40415-024-00990-4
Nadezhda Chukina, Angelina Elkina, Galina Borisova, Olga Sinenko, Irina Kiseleva, Elena Filimonova, Maria Maleva
The study of the rare plant species in technogenically disturbed habitats is a significant prerequisite to preserve their natural populations. Structural and functional characteristics of photosynthetic apparatus and biomass allocation in regionally rare orchid Epipactis atrorubens (Hoffm.) Besser (dark-red helleborine), colonizing two serpentine dumps post-asbestos mining (Anatol’sko-Shilovsky deposit, Sverdlovsk region, Russian), in comparison with the natural forest community were studied. Despite the adverse edaphic conditions (the high stoniness, excess some metals, low content of nutrients, water deficiency), quite numerous populations of E. atrorubens were found in transformed sites. The orchid plants colonizing the serpentine dumps were distinguished by the thicker leaf blade (by 23%), higher leaf mass per area and leaf density (by 36 and 12%, respectively), as well as reduced diffusion resistance to CO2 (by 30%) in comparison with plants in the natural forest community. In addition, the number of cells and chloroplasts per unit leaf area of orchid leaves had increased (by 22% on average). Though orchids grew on serpentine dumps in the unfavorable conditions, the CO2 uptake per unit leaf area and chlorophyll content decreased significantly (by 16 and 40%, respectively) only on one of the dumps, which was characterized by greater stoniness, excess light and water deficiency due to the lack of crown closure. The total fresh and dry biomass of orchid individuals from dumps was slightly less (14% on average) than that in the forest natural community. The underground biomass decreased significantly (26% on average), and at the same time aboveground biomass was higher for plant on dumps. The changes in leaf mesostructure, as well as the maintaining of photosynthetic activity contributed to stability of orchid populations in disturbed habitats. Analysis of the structural adaptations of E. atrorubens indicates that it possess a secondary ecological strategy (competitor–stress-tolerator–ruderal). Thus, the study suggests that plasticity of E. atrorubens photosynthetic apparatus promotes its successful survival in adverse environmental conditions of transformed ecosystems.
{"title":"Photosynthetic apparatus plasticity contributes to successful survival of Epipactis atrorubens (Orchidaceae) in adverse conditions of serpentine dumps","authors":"Nadezhda Chukina, Angelina Elkina, Galina Borisova, Olga Sinenko, Irina Kiseleva, Elena Filimonova, Maria Maleva","doi":"10.1007/s40415-024-00990-4","DOIUrl":"https://doi.org/10.1007/s40415-024-00990-4","url":null,"abstract":"<p>The study of the rare plant species in technogenically disturbed habitats is a significant prerequisite to preserve their natural populations. Structural and functional characteristics of photosynthetic apparatus and biomass allocation in regionally rare orchid <i>Epipactis atrorubens</i> (Hoffm.) Besser (dark-red helleborine), colonizing two serpentine dumps post-asbestos mining (Anatol’sko-Shilovsky deposit, Sverdlovsk region, Russian), in comparison with the natural forest community were studied. Despite the adverse edaphic conditions (the high stoniness, excess some metals, low content of nutrients, water deficiency), quite numerous populations of <i>E. atrorubens</i> were found in transformed sites. The orchid plants colonizing the serpentine dumps were distinguished by the thicker leaf blade (by 23%), higher leaf mass per area and leaf density (by 36 and 12%, respectively), as well as reduced diffusion resistance to CO<sub>2</sub> (by 30%) in comparison with plants in the natural forest community. In addition, the number of cells and chloroplasts per unit leaf area of orchid leaves had increased (by 22% on average). Though orchids grew on serpentine dumps in the unfavorable conditions, the CO<sub>2</sub> uptake per unit leaf area and chlorophyll content decreased significantly (by 16 and 40%, respectively) only on one of the dumps, which was characterized by greater stoniness, excess light and water deficiency due to the lack of crown closure. The total fresh and dry biomass of orchid individuals from dumps was slightly less (14% on average) than that in the forest natural community. The underground biomass decreased significantly (26% on average), and at the same time aboveground biomass was higher for plant on dumps. The changes in leaf mesostructure, as well as the maintaining of photosynthetic activity contributed to stability of orchid populations in disturbed habitats. Analysis of the structural adaptations of <i>E. atrorubens</i> indicates that it possess a secondary ecological strategy (competitor–stress-tolerator–ruderal). Thus, the study suggests that plasticity of <i>E. atrorubens</i> photosynthetic apparatus promotes its successful survival in adverse environmental conditions of transformed ecosystems.</p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139978017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-25DOI: 10.1007/s40415-024-00980-6
Alicia Enriqueta Brechú-Franco, Guillermo Laguna-Hernández, Alejandra Velázquez-Mondragón, Rosa María Fonseca-Juárez, Silvia Espinosa‑Matías
In taxonomic studies, in addition to floral characteristics, the structural characteristics of the vegetative organs also contribute to the taxonomic determination of the species. To provide information regarding these characteristics in the genus Tetramerium, a structural, micromorphological and histochemical analysis of cross sections of the leaves was performed using histochemical techniques and optical and scanning electron microscopy of two of its species, T. glutinosum, endemic to Mexico, and the widely distributed T. tenuissimum, was conducted. The two species presented amphistomatic leaves; double palisade chlorenchyma on adaxial and abaxial surfaces, leaf unifacial; vascular bundle sheaths with kranz anatomy; intradermal and subepidermal cystoliths of various shapes and sizes; nonglandular trichomes osteolate with a thin-walled conical head, glandular trichomes, including a new type, the straight, bright-ringed tricellular trichomes, and a variety of multicellular glandular trichomes. The glandular trichomes secrete waxes or oleoresins and mucilage deposited on the surfaces abaxial and adaxial in the form of platelets, granules and threads or strands. The histochemistry of the cystoliths highlights the presence of proteins and polysaccharides as a product of the possible superposition of the cell wall and plasmalemma lamellae. All these characteristics are typical of species from semi-arid habitats and correspond to the defense function against biotic and abiotic agents assigned to trichomes and their secretions in other studies of various genera and families, as well as to the adaptation function to these habitats of the kranz structure, which was novel for the genus Tetramerium.
在分类学研究中,除了花的特征外,无性器官的结构特征也有助于确定物种的分类。为了提供有关四裂叶属这些特征的信息,研究人员利用组织化学技术对叶片的横截面进行了结构、微形态和组织化学分析,并对其中的两个物种(墨西哥特有的 T. glutinosum 和广泛分布的 T. tenuissimum)进行了光学和扫描电子显微镜观察。这两个物种的叶片为两栖叶;正面和背面均有双栅栏状毛状体,叶片单面;维管束鞘具有克兰兹解剖结构;皮内和表皮下有各种形状和大小的囊石;非腺毛状体呈骨状,头部为薄壁圆锥形;腺毛状体包括一种新类型--直的亮环三细胞毛状体,以及多种多细胞腺毛状体。腺毛体分泌蜡或油脂以及粘液,以板状、颗粒状、线状或股状的形式沉积在背面和正面。囊石的组织化学特征是存在蛋白质和多糖,这可能是细胞壁和质膜叠层的产物。所有这些特征都是半干旱生境物种的典型特征,符合其他不同属和科的研究中赋予毛状体及其分泌物的抵御生物和非生物因子的功能,以及克兰兹结构对这些生境的适应功能,这对四膜属来说是新颖的。
{"title":"Structural characteristics of the leaves of two species of Tetramerium an endemic to Mexico","authors":"Alicia Enriqueta Brechú-Franco, Guillermo Laguna-Hernández, Alejandra Velázquez-Mondragón, Rosa María Fonseca-Juárez, Silvia Espinosa‑Matías","doi":"10.1007/s40415-024-00980-6","DOIUrl":"https://doi.org/10.1007/s40415-024-00980-6","url":null,"abstract":"<p>In taxonomic studies, in addition to floral characteristics, the structural characteristics of the vegetative organs also contribute to the taxonomic determination of the species. To provide information regarding these characteristics in the genus <i>Tetramerium</i>, a structural, micromorphological and histochemical analysis of cross sections of the leaves was performed using histochemical techniques and optical and scanning electron microscopy of two of its species, <i>T. glutinosum</i>, endemic to Mexico, and the widely distributed <i>T. tenuissimum</i>, was conducted. The two species presented amphistomatic leaves; double palisade chlorenchyma on adaxial and abaxial surfaces, leaf unifacial; vascular bundle sheaths with kranz anatomy; intradermal and subepidermal cystoliths of various shapes and sizes; nonglandular trichomes osteolate with a thin-walled conical head, glandular trichomes, including a new type, the straight, bright-ringed tricellular trichomes, and a variety of multicellular glandular trichomes. The glandular trichomes secrete waxes or oleoresins and mucilage deposited on the surfaces abaxial and adaxial in the form of platelets, granules and threads or strands. The histochemistry of the cystoliths highlights the presence of proteins and polysaccharides as a product of the possible superposition of the cell wall and plasmalemma lamellae. All these characteristics are typical of species from semi-arid habitats and correspond to the defense function against biotic and abiotic agents assigned to trichomes and their secretions in other studies of various genera and families, as well as to the adaptation function to these habitats of the kranz structure, which was novel for the genus <i>Tetramerium</i>. </p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139967544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-12DOI: 10.1007/s40415-023-00978-6
Ling Huang, Yuting Zhang, Can Zhou, Fangwei Zhu, Zhuo Lv, Shuguang Wang
As a famous and widely planted shoot-use bamboo species, few researches have been focused on the micropropagation of Dendrocalamus brandisii. This study established an efficient micropropagation system of D. brandisii through seeds and cutoff nodal buds from the multiple shoots in vitro. The orthogonal tests were employed to screen out the optimal disinfection time and hormone combination for multiple shoot induction, proliferation in vitro and root induction in vitro. The results showed that the optimal disinfection time was 10 min. MS medium containing 3 mg L−1 BA, 0.5 mg L−1 KT, 0.10 mg L−1 NAA and 25 g L−1 sucrose showed the highest multiplication rates, and those supplemented with 5 mg L−1 BA, 0.5 mg L−1 KT, 1.0 mg L−1 NAA and 25 g L−1 sucrose were the best for shoot proliferation. 1/2 MS medium supplemented with the hormone combination of 0.1 mg L−1 IBA, and 1.0 mg L−1 NAA was optimal for root induction. For plantlets regeneration from the single nodal buds of multiple shoots, the optimal medium was MS + BA 2.0 mg L−1 + NAA 0.5 mg L−1 for shoot multiplication and 1/2MS + BA 0.1 mg L−1 + NAA 2.0 mg L−1 for root induction. The optimal acclimatization medium was vermiculite + perlite (1:1). This study firstly and systematically established the micropropagation system of D. brandisii by using the seeds and nodal buds of the multiple shoots as explants. Multiple shoots induced from the cutoff nodal buds of the multiple shoots could further expand the micropropagation efficiency.
作为一种著名的、广泛种植的笋用竹种,很少有人关注烙竹的微繁殖研究。本研究通过种子和多梢节芽的离体截取,建立了一种高效的白竹微繁殖系统。采用正交试验筛选出诱导多芽、体外增殖和体外诱导生根的最佳消毒时间和激素组合。结果表明,最佳消毒时间为 10 分钟。含有 3 mg L-1 BA、0.5 mg L-1 KT、0.10 mg L-1 NAA 和 25 g L-1 蔗糖的 MS 培养基繁殖率最高,而添加 5 mg L-1 BA、0.5 mg L-1 KT、1.0 mg L-1 NAA 和 25 g L-1 蔗糖的 MS 培养基芽增殖效果最好。添加了 0.1 mg L-1 IBA 和 1.0 mg L-1 NAA 激素组合的 1/2 MS 培养基对根系诱导效果最佳。对于多芽单节芽的小植株再生,最佳培养基是 MS + BA 2.0 mg L-1 + NAA 0.5 mg L-1 用于芽增殖,1/2MS + BA 0.1 mg L-1 + NAA 2.0 mg L-1 用于根诱导。最佳适应性培养基为蛭石+珍珠岩(1:1)。本研究首次系统地建立了白兰地的微繁殖系统,以种子和多生芽的节芽为外植体。由多肉芽的节芽诱导出的多肉芽可进一步提高微繁殖效率。
{"title":"Establishment of micropropagation system of Dendrocalamus brandisii","authors":"Ling Huang, Yuting Zhang, Can Zhou, Fangwei Zhu, Zhuo Lv, Shuguang Wang","doi":"10.1007/s40415-023-00978-6","DOIUrl":"https://doi.org/10.1007/s40415-023-00978-6","url":null,"abstract":"<p>As a famous and widely planted shoot-use bamboo species, few researches have been focused on the micropropagation of <i>Dendrocalamus brandisii</i>. This study established an efficient micropropagation system of <i>D. brandisii</i> through seeds and cutoff nodal buds from the multiple shoots in vitro. The orthogonal tests were employed to screen out the optimal disinfection time and hormone combination for multiple shoot induction, proliferation in vitro and root induction in vitro. The results showed that the optimal disinfection time was 10 min. MS medium containing 3 mg L<sup>−1</sup> BA, 0.5 mg L<sup>−1</sup> KT, 0.10 mg L<sup>−1</sup> NAA and 25 g L<sup>−1</sup> sucrose showed the highest multiplication rates, and those supplemented with 5 mg L<sup>−1</sup> BA, 0.5 mg L<sup>−1</sup> KT, 1.0 mg L<sup>−1</sup> NAA and 25 g L<sup>−1</sup> sucrose were the best for shoot proliferation. 1/2 MS medium supplemented with the hormone combination of 0.1 mg L<sup>−1</sup> IBA, and 1.0 mg L<sup>−1</sup> NAA was optimal for root induction. For plantlets regeneration from the single nodal buds of multiple shoots, the optimal medium was MS + BA 2.0 mg L<sup>−1</sup> + NAA 0.5 mg L<sup>−1</sup> for shoot multiplication and 1/2MS + BA 0.1 mg L<sup>−1</sup> + NAA 2.0 mg L<sup>−1</sup> for root induction. The optimal acclimatization medium was vermiculite + perlite (1:1). This study firstly and systematically established the micropropagation system of D. brandisii by using the seeds and nodal buds of the multiple shoots as explants. Multiple shoots induced from the cutoff nodal buds of the multiple shoots could further expand the micropropagation efficiency.</p>","PeriodicalId":9140,"journal":{"name":"Brazilian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139754074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-09DOI: 10.1007/s40415-023-00977-7
Elmer Viana Gonçalves, Josiane Celerino de Carvalho, Pedro Paulo dos Santos, Karen Cristina P. da Costa, Adamir da Rocha N. Júnior, Larissa Carolina Alves, Katharine Duarte Gonçalves, Roberval Monteiro B. de Lima, Andreia Varmes Fernandes, Wagner Luiz Araújo, José Francisco de C. Gonçalves
Abstract
The Brazil nut tree (Bertholletia excelsa Bonpl.), one of the main species from the socioeconomic and ecological point of view of the Amazon, is characterized by slow and uneven germination. Considering that its genetic constitution is a determining factor for the morphology and physiology of germination, we investigated different Brazil nut tree genotypes regarding morphophysiological seed traits and performed a morphoanatomical description of the leaves. Genotypes showed differences in seed morphology, imbibition curve, germination rate, and germination speed index, and also, in some of the morphoanatomical leaf structures. The genotype 606 showed the best physiological performance during germination, mainly in terms of water imbibition, as it needs to absorb less water and achieve a higher germination percentage than the genotype Sant Fé, in addition to a higher stomatal index. In the evaluated genotypes, quintenary venation was observed in the leaves, different from a leaf from a wild matrix. Our findings aid the morphoanatomical differentiation of different genotypes of B. excelsa, and represent a significant step forward for clonal propagation, as well as providing a route for improving these species’ physiological and genetic characteristics.