Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220748
Yang Wang, J. Ren, Shangbin Li, Zhijun Hu, Raja Soosaimarian Peter Raj
: Human posture estimation is the basis of many computer vision tasks, such as motion recognition, violence detection and behavior understanding. Therefore, it is of great significance to study the estimation algorithm of human motion posture (HMP). To address the problem of poor estimation effect of traditional HMP estimation algorithm, in this paper, an estimation algorithm for HMP using multi-labeling transfer learning is proposed. First, the original human motion image is labeled by using the multi-label transfer learning, the HMP features are extracted
{"title":"Estimation of Human Motion Posture Using Multi-labeling Transfer Learning","authors":"Yang Wang, J. Ren, Shangbin Li, Zhijun Hu, Raja Soosaimarian Peter Raj","doi":"10.1590/1678-4324-2023220748","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220748","url":null,"abstract":": Human posture estimation is the basis of many computer vision tasks, such as motion recognition, violence detection and behavior understanding. Therefore, it is of great significance to study the estimation algorithm of human motion posture (HMP). To address the problem of poor estimation effect of traditional HMP estimation algorithm, in this paper, an estimation algorithm for HMP using multi-labeling transfer learning is proposed. First, the original human motion image is labeled by using the multi-label transfer learning, the HMP features are extracted","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023230002
Tony Alexandre Medeiros, Raimundo Guimarães Saraiva Junior, G. Cassia, F. Nascimento, João Luiz Azevedo de Carvalho
: Gliomas comprise the vast majority of all malignant brain tumors. Low-grade glioma patients with combined whole-arm losses of 1p and 19q chromosomes
{"title":"Classification of 1p/19q Status in Low-Grade Gliomas: Experiments with Radiomic Features and Ensemble-Based Machine Learning Methods","authors":"Tony Alexandre Medeiros, Raimundo Guimarães Saraiva Junior, G. Cassia, F. Nascimento, João Luiz Azevedo de Carvalho","doi":"10.1590/1678-4324-2023230002","DOIUrl":"https://doi.org/10.1590/1678-4324-2023230002","url":null,"abstract":": Gliomas comprise the vast majority of all malignant brain tumors. Low-grade glioma patients with combined whole-arm losses of 1p and 19q chromosomes","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220297
Kushangi Atrey, B. Singh, N. Bodhey
: The existing practice for Breast Cancer (BC) characterization includes histopathological analysis, which is tedious and time-consuming due to massive data analysis. Further, such techniques are subjected to inter-and intra-observer variability due to the non-availability of skilled pathologists, particularly in low resource settings. Thus, we propose a multi-feature classification technique for risk stratification of BC in Histopathology Images (HI) using machine learning strategies and a Long Short-Term Memory (LSTM) based deep learning approach. Experiments are performed on a publicly available HI database from which a total of 658 image features are extracted, while 192 relevant features are obtained after feature selection using genetic algorithm. The highest accuracy of 99.85% using 192 features under the 5-fold data division protocol is obtained with the LSTM approach. The proposed framework for analyzing HI using multiple grayscale and color features showed promising results and can be an effective tool in the histopathology laboratory.
{"title":"Multi-Feature Classification of Breast Cancer Histopathology Images: An Experimental Investigation in Machine Learning and Deep Learning Paradigm","authors":"Kushangi Atrey, B. Singh, N. Bodhey","doi":"10.1590/1678-4324-2023220297","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220297","url":null,"abstract":": The existing practice for Breast Cancer (BC) characterization includes histopathological analysis, which is tedious and time-consuming due to massive data analysis. Further, such techniques are subjected to inter-and intra-observer variability due to the non-availability of skilled pathologists, particularly in low resource settings. Thus, we propose a multi-feature classification technique for risk stratification of BC in Histopathology Images (HI) using machine learning strategies and a Long Short-Term Memory (LSTM) based deep learning approach. Experiments are performed on a publicly available HI database from which a total of 658 image features are extracted, while 192 relevant features are obtained after feature selection using genetic algorithm. The highest accuracy of 99.85% using 192 features under the 5-fold data division protocol is obtained with the LSTM approach. The proposed framework for analyzing HI using multiple grayscale and color features showed promising results and can be an effective tool in the histopathology laboratory.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220069
H. Noor, M. Sun, A. Ren, P. Ding, Fida Noor, Sana Ullah, Z. Gao
: Wheat is the most widely cultivated food crop on the planet, and it feeds the majority of the world's population. The field experiment was performed from 2019 to 2021 in the eastern part of Loess Plateau, China Nitrogen (N) fertilizer application improves winter wheat yield. It was necessary to analyze the nitrogen utilization of different varieties and screen varieties with high yield and high nitrogen utilization. Six dryland wheat varieties YH-20410, YH-618, YH-805, JM-92, C-6359, and LH-6 were selected to compare the differences in nitrogen uptake and utilization yield, and quality. The aim was to provide theoretical basis for high yield, and high-efficiency wheat production in dryland of southeast loess Plateau. The results showed that the rainfall during the fallow period from 2019 to 2021 was 245.5 mm and 288.9 mm, respectively, and the nitrogen application rate was 180 kg ha –1 .The highest transshipment amount, and transshipment rate of nitrogen before anther were found in YH-618, which reached 79.4 kg ha –1 and 58.6%, respectively. The yield of wheat cultivar YH-20410 was the highest, reaching 4890 kg ha –1 , which was significantly higher than that of YH-805, JM-92, C-6359
{"title":"Nitrogen Use Efficiency and Adaptation of Elite Varieties of Dryland Wheat (Triticum aestivum L.) in the Loess Plateau of China","authors":"H. Noor, M. Sun, A. Ren, P. Ding, Fida Noor, Sana Ullah, Z. Gao","doi":"10.1590/1678-4324-2023220069","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220069","url":null,"abstract":": Wheat is the most widely cultivated food crop on the planet, and it feeds the majority of the world's population. The field experiment was performed from 2019 to 2021 in the eastern part of Loess Plateau, China Nitrogen (N) fertilizer application improves winter wheat yield. It was necessary to analyze the nitrogen utilization of different varieties and screen varieties with high yield and high nitrogen utilization. Six dryland wheat varieties YH-20410, YH-618, YH-805, JM-92, C-6359, and LH-6 were selected to compare the differences in nitrogen uptake and utilization yield, and quality. The aim was to provide theoretical basis for high yield, and high-efficiency wheat production in dryland of southeast loess Plateau. The results showed that the rainfall during the fallow period from 2019 to 2021 was 245.5 mm and 288.9 mm, respectively, and the nitrogen application rate was 180 kg ha –1 .The highest transshipment amount, and transshipment rate of nitrogen before anther were found in YH-618, which reached 79.4 kg ha –1 and 58.6%, respectively. The yield of wheat cultivar YH-20410 was the highest, reaching 4890 kg ha –1 , which was significantly higher than that of YH-805, JM-92, C-6359","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"42 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220558
Zeynep Akşit, H. Gençcelep
: Utilizing food waste is crucial for several reasons, including preserving valuable ingredients, lowering formulation costs, taking advantage of its functional properties, and preserving the environment. Grapefruit waste (GW) and Tomato waste (TW) are used in oil-in-water (O/W) emulsion formulation to investigate their contributions to emulsion stability, textural parameters, and steady shear rheological properties of the model system. O/W emulsions were formed with four different concentrations (0.5%, 1%, 2%, and 3%) of GW and TW, and chicken breast meat and corn oil were the main components of emulsions. Both food wastes increased emulsion stability (ES) in most concentrations compared to the control. The best ES of 86.35 was observed at a concentration of 1% TW emulsion. Emulsions were subjected to texture profile analysis as both row and heat-treated. Heat-treated emulsions had higher hardness values (241.2-518.6 g) than the row emulsions (149.7-247.2 g). All emulsions were found to have pseudoplastic character and exhibit shear thinning behavior. All emulsion samples' apparent viscosity fit well with the Ostwald-de-Waele model (R 2 >0.9). Food waste samples used in the study improved the chicken-type O/W emulsion properties in terms of emulsion stability and textural properties.
{"title":"Using Grapefruit and Tomato Waste in O/W Type Chicken Meat Emulsion","authors":"Zeynep Akşit, H. Gençcelep","doi":"10.1590/1678-4324-2023220558","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220558","url":null,"abstract":": Utilizing food waste is crucial for several reasons, including preserving valuable ingredients, lowering formulation costs, taking advantage of its functional properties, and preserving the environment. Grapefruit waste (GW) and Tomato waste (TW) are used in oil-in-water (O/W) emulsion formulation to investigate their contributions to emulsion stability, textural parameters, and steady shear rheological properties of the model system. O/W emulsions were formed with four different concentrations (0.5%, 1%, 2%, and 3%) of GW and TW, and chicken breast meat and corn oil were the main components of emulsions. Both food wastes increased emulsion stability (ES) in most concentrations compared to the control. The best ES of 86.35 was observed at a concentration of 1% TW emulsion. Emulsions were subjected to texture profile analysis as both row and heat-treated. Heat-treated emulsions had higher hardness values (241.2-518.6 g) than the row emulsions (149.7-247.2 g). All emulsions were found to have pseudoplastic character and exhibit shear thinning behavior. All emulsion samples' apparent viscosity fit well with the Ostwald-de-Waele model (R 2 >0.9). Food waste samples used in the study improved the chicken-type O/W emulsion properties in terms of emulsion stability and textural properties.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220379
Cyntia Cristinne Corrêa Baia de Aquino, T. M. Blasi, C. Unsihuay-Vila, T. Fernandes, Rafael Silva Pinto, Mauro Obladen de Lara Filho, A. R. Aoki, Fabricio H. Tabarro, Rodrigo Braun dos Santos
: The insertion of new distributed energy resources, such as distributed generation (DG), energy storage systems (ESS), demand response (DR), and microgrids (MG), is emerging, bringing new challenges to the current distribution network. In this regard, the active distribution networks (ADN) with multi-microgrids concept appears. The present paper proposes a hierarchical (master-slave problem) computational model to achieve optimal coordinated operation of multi-microgrids connected to an ADN. Day-ahead operation planning of an ADN was formulated as a multiperiod non-linear optimal power flow model, resulting in a non-linear optimization problem, additionally, the day-ahead operation planning of MGs was formulated as a multiperiod linearized optimal power flow resulting in a mixed-integer linear optimization problem. Numerical results on four different test-system microgrids connected to a 359-nodes ADNs test-system belonging to a Brazilian distribution company show the effectiveness of the proposed model and solution strategy. Three cases have been tested: with a maximum load-shedding restriction, without this restriction, and considering insertion of DG. Besides, the hierarchical model can evaluate how much losses and load shedding take effect
{"title":"A Hierarchical Framework for Day-Ahead Optimal Operation Planning of Active Distribution Networks with Multi-Microgrids","authors":"Cyntia Cristinne Corrêa Baia de Aquino, T. M. Blasi, C. Unsihuay-Vila, T. Fernandes, Rafael Silva Pinto, Mauro Obladen de Lara Filho, A. R. Aoki, Fabricio H. Tabarro, Rodrigo Braun dos Santos","doi":"10.1590/1678-4324-2023220379","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220379","url":null,"abstract":": The insertion of new distributed energy resources, such as distributed generation (DG), energy storage systems (ESS), demand response (DR), and microgrids (MG), is emerging, bringing new challenges to the current distribution network. In this regard, the active distribution networks (ADN) with multi-microgrids concept appears. The present paper proposes a hierarchical (master-slave problem) computational model to achieve optimal coordinated operation of multi-microgrids connected to an ADN. Day-ahead operation planning of an ADN was formulated as a multiperiod non-linear optimal power flow model, resulting in a non-linear optimization problem, additionally, the day-ahead operation planning of MGs was formulated as a multiperiod linearized optimal power flow resulting in a mixed-integer linear optimization problem. Numerical results on four different test-system microgrids connected to a 359-nodes ADNs test-system belonging to a Brazilian distribution company show the effectiveness of the proposed model and solution strategy. Three cases have been tested: with a maximum load-shedding restriction, without this restriction, and considering insertion of DG. Besides, the hierarchical model can evaluate how much losses and load shedding take effect","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"16 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.1590/1678-4324-2023220186
S. Doğan, G. Çağlar
: Iris sari Schott ex Baker, an endemic plant, has a natural distribution in Turkey. The plant, also known as “Ana kurtkulağı, Bahar çiçeği” among the people, has showy flowers. Chromosome doubling in the plant was successfully performed with colchicine applied to the micro bulbs of the I. sari obtained in vitro . The different colchicine doses and treatment times tested with I. sari were: 0.1 and 0.5% (2, 4, and 6 hours). The resulting polyploid plantlets were determined by flow cytometric analysis. The highest tetraploid plant yield was observed after 4 h of treatment with 0.1% colchicine. From a total of 45 surviving plantlets, 3 were detected. At the end of the applications, the control and other groups were compared in terms of the viability rates of plants. After 2 subcultures of in vitro conditions, the highest viability rate in plantlets was observed with the control group and 0.1% colchicine for 4 h application, 62.97%, and 67.12%, respectively. Also, I. sari putative tetraploid plantlets had fewer and larger stomata than the control, confirming the data. Although the best ratios in terms of stomata length and width (49.04 and 30.52 mm) were come off after 4 h of 0.5% colchicine treatment, tetraploid was not obtained as a result of this application.
{"title":"Chromosome Doubling in Endemic Iris sari Schott ex Baker with In Vitro Colchicine Treatments","authors":"S. Doğan, G. Çağlar","doi":"10.1590/1678-4324-2023220186","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220186","url":null,"abstract":": Iris sari Schott ex Baker, an endemic plant, has a natural distribution in Turkey. The plant, also known as “Ana kurtkulağı, Bahar çiçeği” among the people, has showy flowers. Chromosome doubling in the plant was successfully performed with colchicine applied to the micro bulbs of the I. sari obtained in vitro . The different colchicine doses and treatment times tested with I. sari were: 0.1 and 0.5% (2, 4, and 6 hours). The resulting polyploid plantlets were determined by flow cytometric analysis. The highest tetraploid plant yield was observed after 4 h of treatment with 0.1% colchicine. From a total of 45 surviving plantlets, 3 were detected. At the end of the applications, the control and other groups were compared in terms of the viability rates of plants. After 2 subcultures of in vitro conditions, the highest viability rate in plantlets was observed with the control group and 0.1% colchicine for 4 h application, 62.97%, and 67.12%, respectively. Also, I. sari putative tetraploid plantlets had fewer and larger stomata than the control, confirming the data. Although the best ratios in terms of stomata length and width (49.04 and 30.52 mm) were come off after 4 h of 0.5% colchicine treatment, tetraploid was not obtained as a result of this application.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.1590/1678-4324-2023220459
Vanessa Barbosa, V. P. Almeida, Jane Manfron, V. Raman, C. F. Oliveira, Fernando Cesar Martins Betim, L. Cruz, J. Swiech, Vinícius Bednarczuk de Oliveira, J. P. Paula, O. G. Miguel
: Phenolic compounds are frequently found in the specific tissues in the leaves and stems of Baccharis species . However, only a few species of the genus have been studied in recent years, including B. erioclada . This study evaluates the in vitro antioxidant potential of B. erioclada crude extract and its fractions. Phytochemical studies, including quantification of total phenolics and flavonoids have also been carried out. Crude extract presented higher antioxidant capacity since it showed lower values of IC50 or greater reductive capacity compared to the fractions. The total phenolic compounds and flavonoids in the HIGHLIGHTS
{"title":"Antioxidant Activity and Determination of Phenolic Compounds, Total Flavonoids and Hispidulin in Baccharis erioclada DC.","authors":"Vanessa Barbosa, V. P. Almeida, Jane Manfron, V. Raman, C. F. Oliveira, Fernando Cesar Martins Betim, L. Cruz, J. Swiech, Vinícius Bednarczuk de Oliveira, J. P. Paula, O. G. Miguel","doi":"10.1590/1678-4324-2023220459","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220459","url":null,"abstract":": Phenolic compounds are frequently found in the specific tissues in the leaves and stems of Baccharis species . However, only a few species of the genus have been studied in recent years, including B. erioclada . This study evaluates the in vitro antioxidant potential of B. erioclada crude extract and its fractions. Phytochemical studies, including quantification of total phenolics and flavonoids have also been carried out. Crude extract presented higher antioxidant capacity since it showed lower values of IC50 or greater reductive capacity compared to the fractions. The total phenolic compounds and flavonoids in the HIGHLIGHTS","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.1590/1678-4324-2023220203
Neslihan Ulubayram, Aycan Yigit Cinar
: Royal Jelly (RJ) is a unique functional food having rich nutrient composition. Due to its extremely sensitive and perishable nature, the cold chain is inevitable to maintain the biological properties of RJ. Microencapsulation is considered as an alternative technology for commercial RJ forms, owing to the elimination of cold-chain requirements. The objective of the study is to evaluate the microencapsulation of RJ and its protective effect on the 10-HDA content as well as on the antimicrobial activity during a defined storage period. Microcapsules were formed by utilizing alginate cross-linking technique in the encapsulator (Buchi B-390, Flawil, Switzerland) under 450 mbar. The antibacterial and antifungal activity of fresh and microencapsulated royal jelly (MRJ) was examined, comparatively. The possible changes in antimicrobial activity in the 1st, 3rd, and 6th months were evaluated considering the changes in 10-HDA levels. The antimicrobial efficiency of RJ on test bacteria ( Micrococcus luteus, Staphylococcus epidermis, Salmonella Enteritidis, Escherichia coli ) and yeast ( Candida albicans, Candida parapsilosis ) was maintained throughout the storage period. On the other hand, antifungal activity on test molds ( Penicillium digitatum, Aspergillus flavus ) slightly decreased from the 3rd month. No significant difference between 10-HDA contents was observed until the end of storage ( p ˃0.05). The results indicate that microencapsulation retains the 10-HDA content of RJ for six months and is a promising method enabling storage at room temperature.
{"title":"Microencapsulated and Fresh Royal Jelly: Monitoring 10-HDA Content, Antibacterial and Antifungal Activity at Different Storage Periods","authors":"Neslihan Ulubayram, Aycan Yigit Cinar","doi":"10.1590/1678-4324-2023220203","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220203","url":null,"abstract":": Royal Jelly (RJ) is a unique functional food having rich nutrient composition. Due to its extremely sensitive and perishable nature, the cold chain is inevitable to maintain the biological properties of RJ. Microencapsulation is considered as an alternative technology for commercial RJ forms, owing to the elimination of cold-chain requirements. The objective of the study is to evaluate the microencapsulation of RJ and its protective effect on the 10-HDA content as well as on the antimicrobial activity during a defined storage period. Microcapsules were formed by utilizing alginate cross-linking technique in the encapsulator (Buchi B-390, Flawil, Switzerland) under 450 mbar. The antibacterial and antifungal activity of fresh and microencapsulated royal jelly (MRJ) was examined, comparatively. The possible changes in antimicrobial activity in the 1st, 3rd, and 6th months were evaluated considering the changes in 10-HDA levels. The antimicrobial efficiency of RJ on test bacteria ( Micrococcus luteus, Staphylococcus epidermis, Salmonella Enteritidis, Escherichia coli ) and yeast ( Candida albicans, Candida parapsilosis ) was maintained throughout the storage period. On the other hand, antifungal activity on test molds ( Penicillium digitatum, Aspergillus flavus ) slightly decreased from the 3rd month. No significant difference between 10-HDA contents was observed until the end of storage ( p ˃0.05). The results indicate that microencapsulation retains the 10-HDA content of RJ for six months and is a promising method enabling storage at room temperature.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"54 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-08DOI: 10.1590/1678-4324-2023220010
Nelzy Neyza Vargas Ramirez de Bazan, Lucas Ranzan, M. Farenzena, J. O. Trierweiler
: Lipids extracted from microalgae for biofuel production have drawn the attention of researchers. Various methods for quantification of neutral lipids, such as solvent extraction or gravimetric, require a large volume of sample and are time-consuming. In this work, Nile Red was employed to evaluate the lipid content in microalgae Scenedesmus sp. through 2D fluorescence spectroscopy using chemometric modeling with a selection of wavelength pairs reducing the variable numbers. Cultivation of microalgae for 10 days was performed. Samples were taken starting after the 5th day of cultivation. Microalgae samples were dried using an oven with tangential airflow and the conventional Bligh and Dyer method for lipid extraction was employed. Before the measurement of fluorescence, the samples were diluted in ethanol at 40°C for 10 minutes, and then Nile Red solution (10 µg/mL ethanol) was added. The results of lipid quantification using the proposed technique achieved mean absolute percentage error (MAPE) lower than 5% and correlation coefficients around 0.99 in the test phase. Linear models using four excitation-emission pairs of fluorescence were fitted and were selected based on Pure Spectral Chemometric Modelling (PSCM). The method for lipid quantification using predictive linear models and 2D fluorescence proved to be robust and fast (requiring less than 30 min) compared to the conventional gravimetric method (which takes at least 5 hours) to obtain comparable results.
{"title":"Rapid Quantification of Lipids in Microalgae Scenedesmus sp. Using Fluorescence","authors":"Nelzy Neyza Vargas Ramirez de Bazan, Lucas Ranzan, M. Farenzena, J. O. Trierweiler","doi":"10.1590/1678-4324-2023220010","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220010","url":null,"abstract":": Lipids extracted from microalgae for biofuel production have drawn the attention of researchers. Various methods for quantification of neutral lipids, such as solvent extraction or gravimetric, require a large volume of sample and are time-consuming. In this work, Nile Red was employed to evaluate the lipid content in microalgae Scenedesmus sp. through 2D fluorescence spectroscopy using chemometric modeling with a selection of wavelength pairs reducing the variable numbers. Cultivation of microalgae for 10 days was performed. Samples were taken starting after the 5th day of cultivation. Microalgae samples were dried using an oven with tangential airflow and the conventional Bligh and Dyer method for lipid extraction was employed. Before the measurement of fluorescence, the samples were diluted in ethanol at 40°C for 10 minutes, and then Nile Red solution (10 µg/mL ethanol) was added. The results of lipid quantification using the proposed technique achieved mean absolute percentage error (MAPE) lower than 5% and correlation coefficients around 0.99 in the test phase. Linear models using four excitation-emission pairs of fluorescence were fitted and were selected based on Pure Spectral Chemometric Modelling (PSCM). The method for lipid quantification using predictive linear models and 2D fluorescence proved to be robust and fast (requiring less than 30 min) compared to the conventional gravimetric method (which takes at least 5 hours) to obtain comparable results.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"145 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67460340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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