Pub Date : 2023-07-03DOI: 10.1590/1678-4324-2023220330
Rathi Karuppasamy, Gomathi Velusamy, Raja Soosaimarian Peter Raj
: This paper aims to improve the quality of reconstructed visual stimuli and reduce the computational complexity of the visual stimuli reconstruction processes in the form of functional Magnetic Resonance Imaging (fMRI) profiles. The preceding work envisions the non-cognitive contents of brain activity vain to integrate visual data of diverse hierarchical levels. Existing approaches such as Deep Canonically Correlated Auto Encoder detect the significant challenges of reconstructing visual stimuli from brain activity: fMRI noise, large dimensionality of a limited number of data instances, and complex structure of visual stimuli. In this activity, we will also analyze the scope for utilizing the spatiotemporal data to resolve the neural correlates of visual stimulus representations and reconstruct the resembling visual stimuli. The purpose of this work is to manipulate those suffering from developmental disabilities.
{"title":"A Novel Approach of Dynamic Vision Reconstruction from fMRI Profiles Using Siamese Conditional Generative Adversarial Network","authors":"Rathi Karuppasamy, Gomathi Velusamy, Raja Soosaimarian Peter Raj","doi":"10.1590/1678-4324-2023220330","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220330","url":null,"abstract":": This paper aims to improve the quality of reconstructed visual stimuli and reduce the computational complexity of the visual stimuli reconstruction processes in the form of functional Magnetic Resonance Imaging (fMRI) profiles. The preceding work envisions the non-cognitive contents of brain activity vain to integrate visual data of diverse hierarchical levels. Existing approaches such as Deep Canonically Correlated Auto Encoder detect the significant challenges of reconstructing visual stimuli from brain activity: fMRI noise, large dimensionality of a limited number of data instances, and complex structure of visual stimuli. In this activity, we will also analyze the scope for utilizing the spatiotemporal data to resolve the neural correlates of visual stimulus representations and reconstruct the resembling visual stimuli. The purpose of this work is to manipulate those suffering from developmental disabilities.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1590/1678-4324-2023210769
I. Singh, Jaswinder Kaur, Sukhanpreet Kaur, B. Barik, R. Pahwa
: Pharmaceutical product development is a challenging, time-consuming, and cost-intensive process. Computational methods could be used for assistance and speed up the industrial process. Artificial neural networks (ANN) and neuro-fuzzy models are tools of artificial intelligence that can be used to develop pharmaceutical products to enhance productivity, quality, and consistency. In the present review, the working principle of ANN and neuro-fuzzy models has been discussed, elaborating on their different types, advantages, and disadvantages. Furthermore, the application of these computational techniques in developing pharmaceutical products like suspension, emulsion, microemulsion, nanocarriers, tablets, transdermal preparations, etc., has been discussed in detail.
{"title":"Artificial Neural Networks and Neuro-Fuzzy Models: Applications in Pharmaceutical Product Development","authors":"I. Singh, Jaswinder Kaur, Sukhanpreet Kaur, B. Barik, R. Pahwa","doi":"10.1590/1678-4324-2023210769","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210769","url":null,"abstract":": Pharmaceutical product development is a challenging, time-consuming, and cost-intensive process. Computational methods could be used for assistance and speed up the industrial process. Artificial neural networks (ANN) and neuro-fuzzy models are tools of artificial intelligence that can be used to develop pharmaceutical products to enhance productivity, quality, and consistency. In the present review, the working principle of ANN and neuro-fuzzy models has been discussed, elaborating on their different types, advantages, and disadvantages. Furthermore, the application of these computational techniques in developing pharmaceutical products like suspension, emulsion, microemulsion, nanocarriers, tablets, transdermal preparations, etc., has been discussed in detail.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"37 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67459653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1590/1678-4324-2023220736
W. G. Vieira Júnior, María Melisa Alberti, A. Pardo-Giménez, M. Iossi, E. S. Dias, D. Zied
: The present work evaluated the agronomic behavior of species and varieties of Pleurotus submitted or not to the scratching technique. The cultivated species/varieties were Pleurotus ostreatus and Pleurotus ostreatus var. Florida, and Pleurotus djamor , where half of the treatments were scratched before the first and second flushes, while the rest were only scratched before the second flush. Yield (%), biological efficiency (%), weight of mushroom (g) and number of mushrooms (uni) were evaluated. It was observed that scratching harmed the mushroom weight for P. ostreatus , where the treatment without scratching produced significantly higher values, in the first harvest flush. On the other hand, scratching favored yield and the number of mushrooms for P. djamor . When the species are compared, it is noted that P. ostreatus obtained a lower yield, which was also reflected in the biological efficiency. P. djamor stood out with a large number of mushrooms, statistically higher than the other species, on the other hand, producing mushrooms of low weight of mushroom. The use of the scratched technique is not recommended for the conditions used in the work, due to the increase in labor and loss of mushroom weight
{"title":"The application of the scratching technique has the same effect on Pleurotus spp.?","authors":"W. G. Vieira Júnior, María Melisa Alberti, A. Pardo-Giménez, M. Iossi, E. S. Dias, D. Zied","doi":"10.1590/1678-4324-2023220736","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220736","url":null,"abstract":": The present work evaluated the agronomic behavior of species and varieties of Pleurotus submitted or not to the scratching technique. The cultivated species/varieties were Pleurotus ostreatus and Pleurotus ostreatus var. Florida, and Pleurotus djamor , where half of the treatments were scratched before the first and second flushes, while the rest were only scratched before the second flush. Yield (%), biological efficiency (%), weight of mushroom (g) and number of mushrooms (uni) were evaluated. It was observed that scratching harmed the mushroom weight for P. ostreatus , where the treatment without scratching produced significantly higher values, in the first harvest flush. On the other hand, scratching favored yield and the number of mushrooms for P. djamor . When the species are compared, it is noted that P. ostreatus obtained a lower yield, which was also reflected in the biological efficiency. P. djamor stood out with a large number of mushrooms, statistically higher than the other species, on the other hand, producing mushrooms of low weight of mushroom. The use of the scratched technique is not recommended for the conditions used in the work, due to the increase in labor and loss of mushroom weight","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"54 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1590/1678-4324-2023220556
Elvis Ricardo de Oliveira, Vanias de Araujo Junior, José Faustino da Silva Cândido, G. Lambert-Torres, Luiz Eduardo Borges da Silva, E. Bonaldi, G. C. C. D. Andrade, Levy Ely de Lacerda de Oliveira, C. H. V. Moraes, Carlos Eduardo Teixeira
: The presented development is an intelligent diagnostic system for transformers that studied machine learning techniques to determine the operational status of these transformers. The study of these techniques is initiated by observing the quantities that define the operational behavior of large transformers, aiming to identify anomalies in their operation from data from sensors that equipment it in the functioning environment. This large power transformer has a theoretical service life of above 20 years and a low failure rate. Thus, obtaining failure values, which have their evolution monitored for large transformers, is almost nil. Therefore, a supervised machine training methodology to diagnose these cases is practically unfeasible. The study carried out with several traditional intelligent techniques can verify this. Several supervised methods (Closest Neighbor K-th Neighbor, Support Vector Machine, Radial Base Function, Decision Trees, Random Forest, Neural Network, AdaBoost, Gaussian Naive Bayes, and Quadratic Discriminant Analysis) were studied.
{"title":"Studying Intelligent Techniques Acting in Large Power Transformer Monitoring","authors":"Elvis Ricardo de Oliveira, Vanias de Araujo Junior, José Faustino da Silva Cândido, G. Lambert-Torres, Luiz Eduardo Borges da Silva, E. Bonaldi, G. C. C. D. Andrade, Levy Ely de Lacerda de Oliveira, C. H. V. Moraes, Carlos Eduardo Teixeira","doi":"10.1590/1678-4324-2023220556","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220556","url":null,"abstract":": The presented development is an intelligent diagnostic system for transformers that studied machine learning techniques to determine the operational status of these transformers. The study of these techniques is initiated by observing the quantities that define the operational behavior of large transformers, aiming to identify anomalies in their operation from data from sensors that equipment it in the functioning environment. This large power transformer has a theoretical service life of above 20 years and a low failure rate. Thus, obtaining failure values, which have their evolution monitored for large transformers, is almost nil. Therefore, a supervised machine training methodology to diagnose these cases is practically unfeasible. The study carried out with several traditional intelligent techniques can verify this. Several supervised methods (Closest Neighbor K-th Neighbor, Support Vector Machine, Radial Base Function, Decision Trees, Random Forest, Neural Network, AdaBoost, Gaussian Naive Bayes, and Quadratic Discriminant Analysis) were studied.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-03DOI: 10.1590/1678-4324-2023220183
D. Sumalapao, Angelyn R. Lao, Athena Acain Adriano, J. So, N. Gloriani
: Continuous heavy rains along with poor road construction and sewage system had resulted in the frequent occurrence of floods in many areas of the Philippines. As a consequence, leptospirosis remains an infectious disease endemic in the Philippines with numerous cases reported as aftermaths of typhoons especially after heavy flooding. Leptospirosis is known for its invasiveness
{"title":"Activation of T-cells and Activity of Macrophages among Smokers with Leptospirosis: a Synergistic Dynamics in the Impairment of Human Immune System","authors":"D. Sumalapao, Angelyn R. Lao, Athena Acain Adriano, J. So, N. Gloriani","doi":"10.1590/1678-4324-2023220183","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220183","url":null,"abstract":": Continuous heavy rains along with poor road construction and sewage system had resulted in the frequent occurrence of floods in many areas of the Philippines. As a consequence, leptospirosis remains an infectious disease endemic in the Philippines with numerous cases reported as aftermaths of typhoons especially after heavy flooding. Leptospirosis is known for its invasiveness","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67461516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220688
Umut Naci Yilmaz, B. Ozturk, Erdal Aglar, O. Saraçoğlu, C. Kaiser
: Both ‘0900 Ziraat’ and ‘Regina’ grafted on ‘Krymsk 5’, or ‘Piku 1’ rootstocks were trained to either Upright Fruiting Offshoots (UFO), Super Slender Axe (SSA) or Kym Green Bush (KGB) training systems. Vegetative growth of the tree, determined by measuring trunk cross-sectional area (TCSA), canopy volume and leaf area, differed significantly, depending on the cultivar x rootstock x training system combination. In general, ‘Krymsk 5’ rootstock resulted in trees with significantly thicker trunks (TCSA: 37.75 cm 2 ) and increased leaf area (up to 86.97 cm 2 ). Fruit weight and fruit quality parameters including Hunter a*, firmness, TSS and acidity were variable between rootstocks and training systems and often not significantly different between treatments. In some years however, significant differences were highly dependent on the training system and rootstock interactions. Higher concentrations of bioactive phytochemical concentrations for total monomeric anthocyanin and antioxidant concentrations were mostly associated with the UFO training system in conjunction with the ‘Krymsk 5’ rootstock suggesting that these are linked to increased tree vigour and increased leaf surface area.
: ' 0900 Ziraat '和' Regina '嫁接在' Krymsk 5 '，或' Piku 1 '砧木被训练成直立果枝(UFO)，超级细长的Axe (SSA)或Kym Green Bush (KGB)训练系统。通过测量树干横截面积(TCSA)、冠层体积和叶面积来测定树木的营养生长，不同品种×砧木×栽培体系组合的差异显著。总体而言，‘克里姆斯克5号’砧木的树干显著变粗(TCSA为37.75 cm 2)，叶面积显著增加(达86.97 cm 2)。果实质量和果实品质参数包括Hunter a*、硬度、TSS和酸度在砧木和训练系统之间存在差异，处理之间通常没有显著差异。然而，在某些年份，显著差异高度依赖于训练系统和砧木的相互作用。较高浓度的总单体花青素生物活性植物化学浓度和抗氧化剂浓度主要与UFO训练系统与“克里姆斯克5号”砧木相关联，表明这些与增加的树木活力和增加的叶表面积有关。
{"title":"Effects of Rootstock and Training System on Tree Canopy, Fruit Quality and Phytochemicals of ‘0900 Ziraat’ and ‘Regina’ Sweet Cherry Cultivars","authors":"Umut Naci Yilmaz, B. Ozturk, Erdal Aglar, O. Saraçoğlu, C. Kaiser","doi":"10.1590/1678-4324-2023220688","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220688","url":null,"abstract":": Both ‘0900 Ziraat’ and ‘Regina’ grafted on ‘Krymsk 5’, or ‘Piku 1’ rootstocks were trained to either Upright Fruiting Offshoots (UFO), Super Slender Axe (SSA) or Kym Green Bush (KGB) training systems. Vegetative growth of the tree, determined by measuring trunk cross-sectional area (TCSA), canopy volume and leaf area, differed significantly, depending on the cultivar x rootstock x training system combination. In general, ‘Krymsk 5’ rootstock resulted in trees with significantly thicker trunks (TCSA: 37.75 cm 2 ) and increased leaf area (up to 86.97 cm 2 ). Fruit weight and fruit quality parameters including Hunter a*, firmness, TSS and acidity were variable between rootstocks and training systems and often not significantly different between treatments. In some years however, significant differences were highly dependent on the training system and rootstock interactions. Higher concentrations of bioactive phytochemical concentrations for total monomeric anthocyanin and antioxidant concentrations were mostly associated with the UFO training system in conjunction with the ‘Krymsk 5’ rootstock suggesting that these are linked to increased tree vigour and increased leaf surface area.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
: Exponential increase in population has led to the release of large amounts of unregulated and untreated wastewater into water bodies such as rivers, lakes, streams, and estuaries causing various environmental and health issues. Traditionally various procedures are applied for the treatment of wastewater at the local and municipality level. Due to the increased need and development of newer technologies, it is necessary to understand all the available procedures and evaluate their efficiency. In the present review, all the important methods involved in the treatment of wastewater and its monitoring have been comprehensively discussed and compared along with their advantages and disadvantages. Information will not only help in understanding the treatment of wastewater but it will also help in developing procedures for the reuse of treated water for various alternative purposes.
{"title":"Sustainable Waste Water Treatment: Opportunities and Challenges","authors":"Sunena Jassal, Rahul Warmoota, Deepti Goyal, Ishita Mittal, Anupama Sharma, Naveen Gupta","doi":"10.1590/1678-4324-2023220546","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220546","url":null,"abstract":": Exponential increase in population has led to the release of large amounts of unregulated and untreated wastewater into water bodies such as rivers, lakes, streams, and estuaries causing various environmental and health issues. Traditionally various procedures are applied for the treatment of wastewater at the local and municipality level. Due to the increased need and development of newer technologies, it is necessary to understand all the available procedures and evaluate their efficiency. In the present review, all the important methods involved in the treatment of wastewater and its monitoring have been comprehensively discussed and compared along with their advantages and disadvantages. Information will not only help in understanding the treatment of wastewater but it will also help in developing procedures for the reuse of treated water for various alternative purposes.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"38 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220626
E. Sholkamy, Sasikumar Palsamy, S. James Raja, K. M. Alarjani, Reem Mohammed Alharbi, N. Abdel-Raouf, K. Alsamhary, V. Duraipandiyan, Fredimoses Mangaladoss, E. Apu, M. Habila
: The main concern of today's agricultural production around the world is a cleaner and greener approach to crop production. Streptomyces species with distinct characteristics can be found in soil. The current research focuses on Streptomyces species isolated from marine sediment samples. To evaluate the morphology and biochemical properties of the isolated Streptomyces strain ukm1, it was grown in Starch Casein Nitrate (SCN) agar medium. Morphological, biochemical, and 16s rRNA analysis were performed on the strain. Streptomyces sp. was identified based on these findings. The production of bioactive metabolites by strain nkm1 was carried out in five different fermentative media. After 10 days of incubation, M6 medium was found to be efficient and was extracted with ethyl acetate. The MIC values revealed that the ethyl acetate extract inhibited the growth of plant pathogenic fungi such as Phytophthora palmivora (31.25 g/ml), Aspergillus flavus (15.62 g/ml), Rhizoctonia solani (31.25 g/ml), and Cladosporium herbarum (31.25 g/ml). The GC-MS analysis of the ethyl acetate extract revealed 26 components, the major volatile bioactive compound being nonadecane.
{"title":"GC-MS Analysis and Bioactivity of Streptomyces sp. nkm1 Volatile Metabolites against some Phytopathogenic Fungi","authors":"E. Sholkamy, Sasikumar Palsamy, S. James Raja, K. M. Alarjani, Reem Mohammed Alharbi, N. Abdel-Raouf, K. Alsamhary, V. Duraipandiyan, Fredimoses Mangaladoss, E. Apu, M. Habila","doi":"10.1590/1678-4324-2023220626","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220626","url":null,"abstract":": The main concern of today's agricultural production around the world is a cleaner and greener approach to crop production. Streptomyces species with distinct characteristics can be found in soil. The current research focuses on Streptomyces species isolated from marine sediment samples. To evaluate the morphology and biochemical properties of the isolated Streptomyces strain ukm1, it was grown in Starch Casein Nitrate (SCN) agar medium. Morphological, biochemical, and 16s rRNA analysis were performed on the strain. Streptomyces sp. was identified based on these findings. The production of bioactive metabolites by strain nkm1 was carried out in five different fermentative media. After 10 days of incubation, M6 medium was found to be efficient and was extracted with ethyl acetate. The MIC values revealed that the ethyl acetate extract inhibited the growth of plant pathogenic fungi such as Phytophthora palmivora (31.25 g/ml), Aspergillus flavus (15.62 g/ml), Rhizoctonia solani (31.25 g/ml), and Cladosporium herbarum (31.25 g/ml). The GC-MS analysis of the ethyl acetate extract revealed 26 components, the major volatile bioactive compound being nonadecane.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023210435
Z. Gharari, K. Bagheri, A. Sharafi
: Scutellaria bornmuelleri is recognized as a medicinal plant and a rich source of terpenoids. Hairy Root Cultures (HRCs) along with elicitation of in vitro cultures have been widely considered in order to produce important secondary metabolites such as terpenoids. In the present study, S. bornmuelleri HRCs were established by transformation of stem explants with Agrobacterium rhizogenes strain A4. The effect of different concentrations of sucrose on transgenic root induction and biomass production were investigated. The production and accumulation of terpenoids in HRCs elicited with chitosan (CS), methyl jasmonate (MeJA) and β -cyclodextrin ( β -CD) was evaluated using Gas chromatography/mass spectrometry (GC/MS). Among various concentrations of sucrose, half-strength Murashige and Skoog (MS) medium supplemented with 2.5% sucrose resulted in significant increase in transformation frequency and maximum root biomass production. The highest sesquiterpene production was observed in HRCs elicited with 100 mg L -1 CS. However, the highest monoterpene accumulation was occurred in HRCs treated with 100 µM MeJA. Among the combined treatments, the highest sesquiterpene and monoterpene yield was achieved by 0.7 and 7 mM β -CD plus MeJA as well as 50 and 100 mg L -1 CS plus MeJA, respectively. To the best of our knowledge, the results of this study for the first time suggested that eliciting HRCs can be an effective method to accumulate sesquiterpenes in large scale cultures of S. bornmuelleri HRs in bioreactor.
{"title":"Enhanced Terpenoids Production of Elicited Hairy Root Cultures of Scutellaria bornmuelleri","authors":"Z. Gharari, K. Bagheri, A. Sharafi","doi":"10.1590/1678-4324-2023210435","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210435","url":null,"abstract":": Scutellaria bornmuelleri is recognized as a medicinal plant and a rich source of terpenoids. Hairy Root Cultures (HRCs) along with elicitation of in vitro cultures have been widely considered in order to produce important secondary metabolites such as terpenoids. In the present study, S. bornmuelleri HRCs were established by transformation of stem explants with Agrobacterium rhizogenes strain A4. The effect of different concentrations of sucrose on transgenic root induction and biomass production were investigated. The production and accumulation of terpenoids in HRCs elicited with chitosan (CS), methyl jasmonate (MeJA) and β -cyclodextrin ( β -CD) was evaluated using Gas chromatography/mass spectrometry (GC/MS). Among various concentrations of sucrose, half-strength Murashige and Skoog (MS) medium supplemented with 2.5% sucrose resulted in significant increase in transformation frequency and maximum root biomass production. The highest sesquiterpene production was observed in HRCs elicited with 100 mg L -1 CS. However, the highest monoterpene accumulation was occurred in HRCs treated with 100 µM MeJA. Among the combined treatments, the highest sesquiterpene and monoterpene yield was achieved by 0.7 and 7 mM β -CD plus MeJA as well as 50 and 100 mg L -1 CS plus MeJA, respectively. To the best of our knowledge, the results of this study for the first time suggested that eliciting HRCs can be an effective method to accumulate sesquiterpenes in large scale cultures of S. bornmuelleri HRs in bioreactor.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67459853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220880
Carlos de Almeida Barbosa, R. Simeoni, Luize Kremer Gamba, V. Ribeiro, M. Cardoso, R. Cunha, C. F. Souza, J. C. Francisco, L. C. Guarita-Souza, B. Fernandes
: The use of biopolymers and platelet-rich fibrin (PRF) is currently investigated as an excellent application biomaterial in the tissue engineering field. Natural biomaterials with application potential in tissue engineering and regenerative medicine are due to their characteristics in biocompatibility, biodegradability, and mechanical characteristic. Though, the basic knowledge of three ‐ dimensional fibrin scaffold and chitosan/alginate processing technology combined with an understanding of the physical-chemical properties of this natural biomaterial is necessary for proper application in regenerative medicine and tissue engineering.
{"title":"Alginate/Chitosan Associates a Platelet-rich in Fibrin Exudates as Drug Delivery Systems in Wounds: a Mini-Review","authors":"Carlos de Almeida Barbosa, R. Simeoni, Luize Kremer Gamba, V. Ribeiro, M. Cardoso, R. Cunha, C. F. Souza, J. C. Francisco, L. C. Guarita-Souza, B. Fernandes","doi":"10.1590/1678-4324-2023220880","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220880","url":null,"abstract":": The use of biopolymers and platelet-rich fibrin (PRF) is currently investigated as an excellent application biomaterial in the tissue engineering field. Natural biomaterials with application potential in tissue engineering and regenerative medicine are due to their characteristics in biocompatibility, biodegradability, and mechanical characteristic. Though, the basic knowledge of three ‐ dimensional fibrin scaffold and chitosan/alginate processing technology combined with an understanding of the physical-chemical properties of this natural biomaterial is necessary for proper application in regenerative medicine and tissue engineering.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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