Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220861
I. H. Çerçi̇, Ömer Faruk Durusoy, Kamil Neyfel Çerçi
: This study aimed to examine the shriveling parameters and some aerobic microbial qualities of waste watermelons in a combined solar energy drying system (CDS) and an open sun drying system (OSDS). Results showed that the CDS was better than the OSDS in terms of the temperature, relative humidity, surface temperature, time, and speed of reaching the target humidity level of the air-shriveled watermelon slices. The number of mesophilic aerobic bacteria (log 10 cfu/g) increased in the shriveled (P>0.05) silages compared to fresh watermelon slices in the CDS and OSDS groups and decreased in silages (P<0.05). Compared to fresh watermelon
{"title":"The Effects of Shriveling up Waste Watermelons in Different Solar Drying Systems on Withering and Relevant Microbial Parameters","authors":"I. H. Çerçi̇, Ömer Faruk Durusoy, Kamil Neyfel Çerçi","doi":"10.1590/1678-4324-2023220861","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220861","url":null,"abstract":": This study aimed to examine the shriveling parameters and some aerobic microbial qualities of waste watermelons in a combined solar energy drying system (CDS) and an open sun drying system (OSDS). Results showed that the CDS was better than the OSDS in terms of the temperature, relative humidity, surface temperature, time, and speed of reaching the target humidity level of the air-shriveled watermelon slices. The number of mesophilic aerobic bacteria (log 10 cfu/g) increased in the shriveled (P>0.05) silages compared to fresh watermelon slices in the CDS and OSDS groups and decreased in silages (P<0.05). Compared to fresh watermelon","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220345
Vilson Roiz Gonçalves Rebelo da Silva, E. Loures, E. Lima, Luiz Antonio Belinaso
: This paper presents the proposition of a set of technical and managerial actions recommended for the management of energy in industry. Economic, environmental, political and social aspects have been putting pressure on the whole society for a more conscious and efficient use and consumption of energy in its diverse forms. Energy-intensive industries show a high energy consumption, they work with processes in severe temperature conditions, residual dissipation of heat and flux of not used residues, always being subject to great environment risks. These large-sized companies offer great
{"title":"Energy Management in Energy Intensive Industries - Recommended Technical & Managerial Actions and a Delphi Study","authors":"Vilson Roiz Gonçalves Rebelo da Silva, E. Loures, E. Lima, Luiz Antonio Belinaso","doi":"10.1590/1678-4324-2023220345","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220345","url":null,"abstract":": This paper presents the proposition of a set of technical and managerial actions recommended for the management of energy in industry. Economic, environmental, political and social aspects have been putting pressure on the whole society for a more conscious and efficient use and consumption of energy in its diverse forms. Energy-intensive industries show a high energy consumption, they work with processes in severe temperature conditions, residual dissipation of heat and flux of not used residues, always being subject to great environment risks. These large-sized companies offer great","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023220358
Elif Erturk, O. Onur, İ. Aydın, M. Z. Ozel, Mehmet Firat, F. Ari
: Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer
{"title":"Cytotoxic Potential of Rare Plant Salvia candidissima subsp. candidissima on Breast Cancer Cells","authors":"Elif Erturk, O. Onur, İ. Aydın, M. Z. Ozel, Mehmet Firat, F. Ari","doi":"10.1590/1678-4324-2023220358","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220358","url":null,"abstract":": Breast cancer is the leading cause of cancer-related deaths in women throughout the world. Research on natural anti-cancer products from plants has gained traction. Salvia L. species and their derivatives are rare in Turkey and have suggested for their potential anti-cancer","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-19DOI: 10.1590/1678-4324-2023210014
Ana Paula Nogueira Ferri, Bruna Gomide, Fellipe Augusto Tocchini de Figueiredo, C. L. Carta, I. Balducci, J. Almeida
: The objective of the present work was to quantify the effect of phenolic derivatives on in the oral mucosa of university laboratory technicians. Twenty (20) university laboratory technicians exposed to agents and 11 non-smokers without occupational exposure to phenolic derivatives for more than 10 years underwent anamnesis and extra and intra-oral clinical examination. Exfoliative cytology of the right buccal mucosa was performed. The frequency of micronuclei formation (MN) was analyzed in 1200 cells by standard microscopy. Mann-Whitney and Kruskal-Wallis test were used. The difference between the groups evaluated was observed in the frequency of more than 01 MN/cell (p=0.0397), being more present in the exposed group. The use of personal protective equipment contributed to a decrease in the frequency of 01 MN/cell (p=0.0272) for the exposed group. Genotoxic effects may be reduced by the use of PPE and/or exhaust laminar flow hood. Five years of exposure could adapt the nucleus cells to the genotoxic aggressor demonstrating that the oral mucosa could be a marker to long-term genotoxic exposure.
{"title":"Effect of Phenol Derivatives in the Oral Mucosa of University Laboratory Technicians","authors":"Ana Paula Nogueira Ferri, Bruna Gomide, Fellipe Augusto Tocchini de Figueiredo, C. L. Carta, I. Balducci, J. Almeida","doi":"10.1590/1678-4324-2023210014","DOIUrl":"https://doi.org/10.1590/1678-4324-2023210014","url":null,"abstract":": The objective of the present work was to quantify the effect of phenolic derivatives on in the oral mucosa of university laboratory technicians. Twenty (20) university laboratory technicians exposed to agents and 11 non-smokers without occupational exposure to phenolic derivatives for more than 10 years underwent anamnesis and extra and intra-oral clinical examination. Exfoliative cytology of the right buccal mucosa was performed. The frequency of micronuclei formation (MN) was analyzed in 1200 cells by standard microscopy. Mann-Whitney and Kruskal-Wallis test were used. The difference between the groups evaluated was observed in the frequency of more than 01 MN/cell (p=0.0397), being more present in the exposed group. The use of personal protective equipment contributed to a decrease in the frequency of 01 MN/cell (p=0.0272) for the exposed group. Genotoxic effects may be reduced by the use of PPE and/or exhaust laminar flow hood. Five years of exposure could adapt the nucleus cells to the genotoxic aggressor demonstrating that the oral mucosa could be a marker to long-term genotoxic exposure.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67459615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220427
Iliasu Alhassan, H. Çoklar, M. Akbulut
: The main aim of this study was to determine the changes in quality properties and bioactive compounds of 3 crab apple species ( Malus evereste, Malus floribunda and Malus floribunda coccinella ) harvested at 5 different edible maturity stages. Acidity, soluble solids, organic acid and sugar contents were determined in the whole apple. The fleshes and peels of the crab apples were analyzed for antioxidant activities, color, protein, total monomeric anthocyanin, and total phenolic contents. Maturity stage had no significant effect on size, pH, titratable acidity, and citric acid content of crab apples. Peels of all three species had higher total phenolic, monomeric anthocyanin, tannin, and protein concentrations than their fleshes. M. evereste was the richest source of total phenolic and tannin among the species and it exhibited the highest antioxidant activity. Unlike the total phenolic compounds, anthocyanin accumulation was the highest in the flesh of M. floribunda . Concentrations of sugars in all species increased throughout the maturity. While the highest levels of glucose and fructose determined in M. evereste at the last stage, sucrose concentration was higher in M. floribunda that those in other two species at all stages. The trend in malic acid accumulation showed differences between the species. As a result, it can be concluded that the fruits of all species can be harvested in the first two weeks of September.
{"title":"Determination of Some Physical, Chemical and Nutritional Properties in the Peel and Flesh of Three Crab Apple Species at Five Edible Maturity Stages","authors":"Iliasu Alhassan, H. Çoklar, M. Akbulut","doi":"10.1590/1678-4324-2023220427","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220427","url":null,"abstract":": The main aim of this study was to determine the changes in quality properties and bioactive compounds of 3 crab apple species ( Malus evereste, Malus floribunda and Malus floribunda coccinella ) harvested at 5 different edible maturity stages. Acidity, soluble solids, organic acid and sugar contents were determined in the whole apple. The fleshes and peels of the crab apples were analyzed for antioxidant activities, color, protein, total monomeric anthocyanin, and total phenolic contents. Maturity stage had no significant effect on size, pH, titratable acidity, and citric acid content of crab apples. Peels of all three species had higher total phenolic, monomeric anthocyanin, tannin, and protein concentrations than their fleshes. M. evereste was the richest source of total phenolic and tannin among the species and it exhibited the highest antioxidant activity. Unlike the total phenolic compounds, anthocyanin accumulation was the highest in the flesh of M. floribunda . Concentrations of sugars in all species increased throughout the maturity. While the highest levels of glucose and fructose determined in M. evereste at the last stage, sucrose concentration was higher in M. floribunda that those in other two species at all stages. The trend in malic acid accumulation showed differences between the species. As a result, it can be concluded that the fruits of all species can be harvested in the first two weeks of September.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"275 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220261
G. Misra, Sandhya Hora, Sanjana Ginwal, Neeraj Singh, Anupkuamar Anvikar
The Severe Acute Respiratory Syndrome Coronavirus-2 causes a dreadful Coronavirus Disease namely COVID-19. Respiratory system is the primary target of the virus. It also impairs other major organs such as kidney, heart, liver, brain etc. Multiple novel variants of SARS-CoV-2 have appeared since the SARS-CoV-2 pandemic occurred which are linked to increased virulence, disease transmission and severity. The virus attacks the host signalling pathways to maintain a favourable environment for its spread. The present study focuses on the comprehensive analysis of major signaling pathways affected due to several variants of SARS-CoV-2 leading to abnormalities in cell growth and differentiation. The information was curated from the weblinks of several platforms like WHO, CDC, PANGO, Nextstrain clade and GISAID clade. The data on signaling pathways and comorbidities was generated by screening of different research and review articles. SARS-CoV-2 consolidates the cytoskeleton of the host for effective cell invasion and modulates the transcription processes to enable the translation of viral protein(s). These events lead to significant increase and prolonged hyper inflammation. Further, a decreased interferon (IFN) response along with increased interleukin production leading to cytokine storm is observed. Deregulation of interleukin pathways, TNF-alpha signalling through JAK/STAT-3 signalling, MAPK1, mTOR, PI3K are few other signalling pathways that are affected on SARS-CoV-2 infection. This review represents a comprehensive analysis of the vigorous life cycle of SARS CoV-2, its different variants affecting host signalling pathways which eventually cause dysfunctioning of several organs and development of comorbidities.
{"title":"SARS-CoV-2 Variants Impact on Key Signaling Pathways Metamorphoses into Severity","authors":"G. Misra, Sandhya Hora, Sanjana Ginwal, Neeraj Singh, Anupkuamar Anvikar","doi":"10.1590/1678-4324-2023220261","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220261","url":null,"abstract":"The Severe Acute Respiratory Syndrome Coronavirus-2 causes a dreadful Coronavirus Disease namely COVID-19. Respiratory system is the primary target of the virus. It also impairs other major organs such as kidney, heart, liver, brain etc. Multiple novel variants of SARS-CoV-2 have appeared since the SARS-CoV-2 pandemic occurred which are linked to increased virulence, disease transmission and severity. The virus attacks the host signalling pathways to maintain a favourable environment for its spread. The present study focuses on the comprehensive analysis of major signaling pathways affected due to several variants of SARS-CoV-2 leading to abnormalities in cell growth and differentiation. The information was curated from the weblinks of several platforms like WHO, CDC, PANGO, Nextstrain clade and GISAID clade. The data on signaling pathways and comorbidities was generated by screening of different research and review articles. SARS-CoV-2 consolidates the cytoskeleton of the host for effective cell invasion and modulates the transcription processes to enable the translation of viral protein(s). These events lead to significant increase and prolonged hyper inflammation. Further, a decreased interferon (IFN) response along with increased interleukin production leading to cytokine storm is observed. Deregulation of interleukin pathways, TNF-alpha signalling through JAK/STAT-3 signalling, MAPK1, mTOR, PI3K are few other signalling pathways that are affected on SARS-CoV-2 infection. This review represents a comprehensive analysis of the vigorous life cycle of SARS CoV-2, its different variants affecting host signalling pathways which eventually cause dysfunctioning of several organs and development of comorbidities.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220381
Shayesteh Shookouh-Zangeneh, M. Esmaelizad, Aylar Safari, Nazanin Esmaelizad
: Mycoplasma agalactiae is an economically important pathogen and the main etiological agent of contagious agalactiae (CA) in small ruminants with a relatively high prevalence in sheep and goat, mainly characterized by mastitis, conjunctivitis and arthritis as predominant symptoms of a localized infection. Two genes encoding p48 and p40 major surface lipoprotein are played a fundamental role in the pathogenesis of
{"title":"Designing a P48-40 Chimeric Protein of Mycoplasma agalactiae and Highly Expression in E. coli, Applicable for Indirect ELISA","authors":"Shayesteh Shookouh-Zangeneh, M. Esmaelizad, Aylar Safari, Nazanin Esmaelizad","doi":"10.1590/1678-4324-2023220381","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220381","url":null,"abstract":": Mycoplasma agalactiae is an economically important pathogen and the main etiological agent of contagious agalactiae (CA) in small ruminants with a relatively high prevalence in sheep and goat, mainly characterized by mastitis, conjunctivitis and arthritis as predominant symptoms of a localized infection. Two genes encoding p48 and p40 major surface lipoprotein are played a fundamental role in the pathogenesis of","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67462657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220750
Ş. Önalan, M. Bulut, D. Alwazeer
: This study aimed to evaluate the use of an oxy-hydrogen generator for preparing hydrogen-rich water (HRW) and studying its effects on the L. garvieae-affected zebrafish. 0, 10, 20, and 100% HRW were prepared to determine the effects of HRW on the mortality rate and gene expression levels of L. garvieae infected-zebrafish. After 48 hours of bacterial injection, the mortality rate of fish was 0, 0, 0, 66, 80
{"title":"Evaluation of the Potential Use of Oxy-Hydrogen Gas for the Treatment of Lactococcus garvieae Infected- Zebrafish in Hydrogen-Rich Water Aquarium","authors":"Ş. Önalan, M. Bulut, D. Alwazeer","doi":"10.1590/1678-4324-2023220750","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220750","url":null,"abstract":": This study aimed to evaluate the use of an oxy-hydrogen generator for preparing hydrogen-rich water (HRW) and studying its effects on the L. garvieae-affected zebrafish. 0, 10, 20, and 100% HRW were prepared to determine the effects of HRW on the mortality rate and gene expression levels of L. garvieae infected-zebrafish. After 48 hours of bacterial injection, the mortality rate of fish was 0, 0, 0, 66, 80","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220803
A. Beyaz, Veysel Gül
: The decrease in the possibilities of increasing the arable agricultural areas in the world and the continuous increase in the population have led those who are engaged in plant production to seek ways to make maximum use of the existing agricultural areas. One of these ways is mixed sowing systems. It is very difficult to sow species with different grain sizes in mixtures. Special sowing machines are needed for this aim. Because of this reason, the article aims to be a guide for artificial intelligence capable of mixed sowing in forage crops. In the research, it is found that there are some differences between YOLOv4-tiny and Y0L0v4 models as Precision, Recall, F1-score, TP, FP, FN scores. For the YOLOv4-tiny model, these scores were found as 0.99, 1.00, 0.99, 90, 1, 0, respectively and the scores for the Y0L0v4 model were 1.00, 1.00, 1.00, 90, 0, 0. According to the YOLOv4-tiny and YOLOv4 tests in the lab, suggesting that the YOLOv4-tiny is faster, and the YOLOv4 is more reliable in terms of all these factors combined. This research establishes a standard for real-time recognition of forage crops based on current technology at NVIDIA Jetson TX2 due to its high performance and low power consumption and a high-performance computer with CUDA support.
{"title":"YOLOv4 and Tiny YOLOv4 Based Forage Crop Detection with an Artificial Intelligence Board","authors":"A. Beyaz, Veysel Gül","doi":"10.1590/1678-4324-2023220803","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220803","url":null,"abstract":": The decrease in the possibilities of increasing the arable agricultural areas in the world and the continuous increase in the population have led those who are engaged in plant production to seek ways to make maximum use of the existing agricultural areas. One of these ways is mixed sowing systems. It is very difficult to sow species with different grain sizes in mixtures. Special sowing machines are needed for this aim. Because of this reason, the article aims to be a guide for artificial intelligence capable of mixed sowing in forage crops. In the research, it is found that there are some differences between YOLOv4-tiny and Y0L0v4 models as Precision, Recall, F1-score, TP, FP, FN scores. For the YOLOv4-tiny model, these scores were found as 0.99, 1.00, 0.99, 90, 1, 0, respectively and the scores for the Y0L0v4 model were 1.00, 1.00, 1.00, 90, 0, 0. According to the YOLOv4-tiny and YOLOv4 tests in the lab, suggesting that the YOLOv4-tiny is faster, and the YOLOv4 is more reliable in terms of all these factors combined. This research establishes a standard for real-time recognition of forage crops based on current technology at NVIDIA Jetson TX2 due to its high performance and low power consumption and a high-performance computer with CUDA support.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67464443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-22DOI: 10.1590/1678-4324-2023220591
Larissa Glugoski, Laís Priscila Karas, V. Nogaroto, Fernanda Couto Miléo, Ana Luiza Augustinho, Mackelly Simionatto, Marcos Pileggi, B. R. Cruz, G. M. Fávero, M. R. Vicari
: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the responsible for the coronavirus disease 2019 (COVID-19) pandemic. Because of its high transmissibility, allied to the emergence of some variants of concern (VOC), like Alpha, Beta, Gamma, Delta, and Omicron, the detection of these strains must be rapid and efficient. Among the tests available, the reverse-transcriptase polymerase-chain reaction (RT-PCR) is considered the gold standard test for COVID-19 detection, and the use of specific primers and probes can discriminate the different COVID-19 variants. In this study, we screened 317 individuals tested positive for COVID-19 from the municipality of Ponta Grossa (Paraná State, Brazil), from April/2021 to February/2022, aiming to identify the Gamma, Delta, and Omicron variants, by RT-PCR, using specific probes. The Gamma variant was detected from April/2021 to September/2021. The Delta variant was subsequently detected from August/2021 to November/2021. The Omicron variant was the unique strain detected from December/2021 to February/2022 and, because of its high rate of transmissibility, it caused a considerable increase in the number of COVID-19 infections. Mass testing is considered an important strategy for COVID-19 control and, the correct detection of new strains, could improve the knowledge about the virus´ behavior, permitting the development of drugs and optimized vaccines.
{"title":"Monitoring the Circulation and Impact of SARS-Cov-2 Variants on Public Health During COVID-19 Pandemic: a Case Study in a South Brazil Population","authors":"Larissa Glugoski, Laís Priscila Karas, V. Nogaroto, Fernanda Couto Miléo, Ana Luiza Augustinho, Mackelly Simionatto, Marcos Pileggi, B. R. Cruz, G. M. Fávero, M. R. Vicari","doi":"10.1590/1678-4324-2023220591","DOIUrl":"https://doi.org/10.1590/1678-4324-2023220591","url":null,"abstract":": Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was the responsible for the coronavirus disease 2019 (COVID-19) pandemic. Because of its high transmissibility, allied to the emergence of some variants of concern (VOC), like Alpha, Beta, Gamma, Delta, and Omicron, the detection of these strains must be rapid and efficient. Among the tests available, the reverse-transcriptase polymerase-chain reaction (RT-PCR) is considered the gold standard test for COVID-19 detection, and the use of specific primers and probes can discriminate the different COVID-19 variants. In this study, we screened 317 individuals tested positive for COVID-19 from the municipality of Ponta Grossa (Paraná State, Brazil), from April/2021 to February/2022, aiming to identify the Gamma, Delta, and Omicron variants, by RT-PCR, using specific probes. The Gamma variant was detected from April/2021 to September/2021. The Delta variant was subsequently detected from August/2021 to November/2021. The Omicron variant was the unique strain detected from December/2021 to February/2022 and, because of its high rate of transmissibility, it caused a considerable increase in the number of COVID-19 infections. Mass testing is considered an important strategy for COVID-19 control and, the correct detection of new strains, could improve the knowledge about the virus´ behavior, permitting the development of drugs and optimized vaccines.","PeriodicalId":9169,"journal":{"name":"Brazilian Archives of Biology and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67463905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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