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Reconfigurable Antenna for Wi-Fi and 5G Applications 适用于Wi-Fi和5G应用的可重构天线
Pub Date : 2023-05-15 DOI: 10.1016/j.promfg.2020.04.007
Doae El Hadri, A. Zakriti, A. Zugari
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引用次数: 7
Identification and structural basis of an enzyme that degrades oligosaccharides in caramel. 鉴定一种降解焦糖中低聚糖的酶及其结构基础。
Pub Date : 2023-03-29 eCollection Date: 2023-01-01 DOI: 10.2142/biophysico.bppb-v20.0017
Toma Kashima, Akihiro Ishiwata, Kiyotaka Fujita, Shinya Fushinobu

Cooking with fire produces foods containing carbohydrates that are not naturally occurring, such as α-d-fructofuranoside found in caramel. Each of the hundreds of compounds produced by caramelization reactions is considered to possess its own characteristics. Various studies from the viewpoints of biology and biochemistry have been conducted to elucidate some of the scientific characteristics. Here, we review the composition of caramelized sugars and then describe the enzymatic studies that have been conducted and the physiological functions of the caramelized sugar components that have been elucidated. In particular, we recently identified a glycoside hydrolase (GH), GH172 difructose dianhydride I synthase/hydrolase (αFFase1), from oral and intestinal bacteria, which is implicated in the degradation of oligosaccharides in caramel. The structural basis of αFFase1 and its ligands provided many insights. This discovery opened the door to several research fields, including the structural and phylogenetic relationship between the GH172 family enzymes and viral capsid proteins and the degradation of cell membrane glycans of acid-fast bacteria by some αFFase1 homologs. This review article is an extended version of the Japanese article, Identification and Structural Basis of an Enzyme Degrading Oligosaccharides in Caramel, published in SEIBUTSU BUTSURI Vol. 62, p. 184-186 (2022).

用火烹饪会产生含有非天然碳水化合物的食物,例如焦糖中的α-d-呋喃果糖苷。焦糖化反应产生的数百种化合物被认为各具特色。为了阐明其中的一些科学特征,人们从生物学和生物化学的角度进行了各种研究。在此,我们回顾了焦糖的组成,然后介绍了已开展的酶学研究和已阐明的焦糖成分的生理功能。特别是,我们最近从口腔和肠道细菌中发现了一种糖苷水解酶(GH),即 GH172 二果糖二酐 I 合成酶/水解酶(αFFase1),它与焦糖中低聚糖的降解有关。αFFase1及其配体的结构基础提供了许多见解。这一发现为多个研究领域打开了大门,包括 GH172 家族酶与病毒帽蛋白之间的结构和系统发育关系,以及一些 αFFase1 同源物对酸性无菌细菌细胞膜糖的降解作用。本综述文章是日文文章《Identification and Structural Basis of an Enzyme Degrading Oligosaccharides in Caramel》(焦糖低聚糖降解酶的鉴定与结构基础)的扩展版,发表于《科学文摘》(SEIBUTSU BUTSURI)第 62 卷第 184-186 页(2022 年)。
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引用次数: 0
Combined proteomic and biochemical analyses redefine the consensus sequence requirement for epidermal growth factor-like domain hydroxylation. 蛋白质组学和生化分析重新定义了表皮生长因子样结构域羟基化的共识序列要求。
Pub Date : 2022-08-01 Epub Date: 2022-06-11 DOI: 10.1016/j.jbc.2022.102129
Lennart Brewitz, Bruce C Onisko, Christopher J Schofield

Epidermal growth factor-like domains (EGFDs) have important functions in cell-cell signaling. Both secreted and cell surface human EGFDs are subject to extensive modifications, including aspartate and asparagine residue C3-hydroxylations catalyzed by the 2-oxoglutarate oxygenase aspartate/asparagine-β-hydroxylase (AspH). Although genetic studies show AspH is important in human biology, studies on its physiological roles have been limited by incomplete knowledge of its substrates. Here, we redefine the consensus sequence requirements for AspH-catalyzed EGFD hydroxylation based on combined analysis of proteomic mass spectrometric data and mass spectrometry-based assays with isolated AspH and peptide substrates. We provide cellular and biochemical evidence that the preferred site of EGFD hydroxylation is embedded within a disulfide-bridged macrocycle formed of 10 amino acid residues. This definition enabled the identification of previously unassigned hydroxylation sites in three EGFDs of human fibulins as AspH substrates. A non-EGFD containing protein, lymphocyte antigen-6/plasminogen activator urokinase receptor domain containing protein 6B (LYPD6B) was shown to be a substrate for isolated AspH, but we did not observe evidence for LYPD6B hydroxylation in cells. AspH-catalyzed hydroxylation of fibulins is of particular interest given their important roles in extracellular matrix dynamics. In conclusion, these results lead to a revision of the consensus substrate requirements for AspH and expand the range of observed and potential AspH-catalyzed hydroxylation in cells, which will enable future study of the biological roles of AspH.

表皮生长因子样结构域(EGFDs)在细胞-细胞信号传导中具有重要功能。分泌型和细胞表面的人类表皮生长因子样结构域都会受到广泛的修饰,包括由天冬氨酸/天冬酰胺-β-羟化酶(AspH)催化的天冬氨酸和天冬酰胺残基 C3-羟化。尽管基因研究表明 AspH 在人类生物学中非常重要,但由于对其底物的了解不全面,对其生理作用的研究一直受到限制。在这里,我们根据对蛋白质组质谱数据的综合分析以及基于质谱的分离 AspH 和肽底物的测定,重新定义了 AspH 催化 EGFD 羟基化的共识序列要求。我们提供的细胞和生物化学证据表明,EGFD 羟基化的首选位点位于由 10 个氨基酸残基组成的二硫键大循环中。根据这一定义,人类纤维蛋白的三个 EGFD 中以前未指定的羟化位点被鉴定为 AspH 底物。一种不含 EGFD 的蛋白质--含淋巴细胞抗原-6/浆细胞酶原激活剂尿激酶受体结构域的蛋白质 6B(LYPD6B)被证明是分离的 AspH 的底物,但我们在细胞中没有观察到 LYPD6B 羟基化的证据。鉴于纤维素在细胞外基质动力学中的重要作用,AspH 催化的纤维素羟基化尤其令人感兴趣。总之,这些结果导致了对 AspH 共识底物要求的修订,并扩大了在细胞中观察到的和潜在的 AspH 催化羟化作用的范围,这将有助于未来对 AspH 生物作用的研究。
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引用次数: 0
Management of COVID-19 Cases in Kosova. 科索沃COVID-19病例的管理
Pub Date : 2022-06-13 eCollection Date: 2022-06-01 DOI: 10.36519/idcm.2022.125
Salih Ahmeti, Şiran Keske, Sadije Namani-Avdiu, Lindita Ajazaj-Berisha, Arben Vishaj, Izet Sadik, Mentor Alimusaj, Faik Hoti, Hysen Ahmeti, Bilgin Sait, Nahit Çakar, Önder Ergönül
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引用次数: 0
Implementing Digitalised Lean Manufacturing Training in a UK Engine Manufacturing Centre During the SARS-CoV2 Pandemic of 2020 2020年SARS-CoV2大流行期间在英国发动机制造中心实施数字化精益制造培训
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.10.078
M.G. McKie , R. Jones , J. Miles , I.R. Jones

This paper examines a novel way of training Lean Manufacturing Systems and Tools utilising an Industry 4.0 methodology during the SARS-COVID2 Pandemic of 2020. Currently, it is challenging for the Integrated Production Systems Team, responsible for carrying out training on the Lean principles, to undertake the training safely and without the risk of possible disease transmission. This is due to the usual close quarters training carried out in the Engine Manufacturing Centre. Schools, Colleges and Universities have adapted and utilised technology and moved to an Industry 4.0 digitalised approach to learning and development. This is therefore an opportunity for manufacturing to follow suit and create digitised solutions to training and development opportunities, to ensure that the employees within the manufacturing facility have adequate knowledge on the Lean principles.

本文探讨了在2020年sars - covid - 2大流行期间利用工业4.0方法培训精益制造系统和工具的新方法。目前,对于负责开展精益原则培训的综合生产系统小组来说,在没有可能的疾病传播风险的情况下安全开展培训是一项挑战。这是由于通常在发动机制造中心进行的近距离训练。学校、学院和大学已经适应和利用技术,并转向工业4.0的数字化学习和发展方法。因此,这是制造业效仿和创建数字化解决方案的机会,以提供培训和发展机会,以确保生产设施内的员工对精益原则有足够的了解。
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引用次数: 2
Propagation of Johnson-Cook flow stress model uncertainty to milling force uncertainty using finite element analysis and time domain simulation 基于有限元分析和时域仿真的Johnson-Cook流变应力模型不确定性向铣削力不确定性的传递
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.06.025
Timothy No , Michael Gomez , Jaydeep Karandikar , Jarred Heigel , Ryan Copenhaver , Tony Schmitz

This paper describes the propagation of uncertainty in the parameters for a 6061-T6 aluminum Johnson-Cook flow stress model to, first, the uncertainty in the corresponding mechanistic cutting force model obtained by orthogonal cutting finite element simulation and, second, the milling force predicted by time domain simulation using the force model. The approach includes five key elements: 1) a literature review to identify the means and standard deviations for the 6061-T6 aluminum Johnson-Cook model parameters; 2) structured light scanning to measure an endmill’s cutting edge macro-geometry along the tool axis; 3) structured light scanning to identify the cutting edge cross-sectional rake and relief profiles for the same endmill; 4) orthogonal cutting finite element analysis to determine the mechanistic force model coefficients that relate the force components to chip area and width using the tool’s rake and relief profiles and random samples from the Johnson-Cook parameter distributions; and 5) time domain simulation with inputs that include the measured cutting edge macro-geometry, uncertain finite element-based force model, and measured structural dynamics. Distributions for milling force predictions are determined by Monte Carlo simulation and compared to in-process measurements for an indexable endmill-collet holder to demonstrate the approach. It is observed that 95% confidence intervals on the predicted forces bound the measured time-dependent force profile peaks in over half of the cases tested. It is also seen that the Johnson-Cook flow stress model-based force predictions performed as well as predictions based on a calibrated mechanistic force model.

本文描述了6061-T6铝合金Johnson-Cook流变应力模型参数的不确定性传递到:一是正交切削有限元模拟得到的相应切削力力学模型的不确定性,二是利用切削力模型进行时域仿真预测的铣削力。该方法包括五个关键要素:1)通过文献综述,确定6061-T6铝Johnson-Cook模型参数的均值和标准差;2)结构光扫描测量立铣刀沿刀轴的刃口宏观几何形状;3)结构光扫描识别同一立铣刀的切削刃横截面前刀和后刀轮廓;4)通过正交切削有限元分析,利用刀具的前倾和后倾轮廓以及Johnson-Cook参数分布的随机样本,确定力分量与切屑面积和切屑宽度相关的机械力模型系数;5)时域仿真,输入包括测量的前沿宏观几何、不确定有限元力模型和测量的结构动力学。铣削力预测的分布由蒙特卡罗模拟确定,并与可转位立铣刀夹头的过程测量进行比较,以证明该方法。可以观察到,在超过一半的测试案例中,预测力的95%置信区间约束了测量的随时间变化的力剖面峰值。此外,基于Johnson-Cook流动应力模型的力预测效果与基于校准的机械力模型的预测效果相同。
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引用次数: 3
Estimation of cBN grinding wheel condition using image sensor 基于图像传感器的cBN砂轮状态估计
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.06.031
Eddie Taewan Lee, Zhaoyan Fan, Burak Sencer

The tool condition used in machining process is generally determined by the wear status, which is an important factor of resulting in the machining efficiency of the manufacturing processes. This paper presents a method to estimate the tool condition of grinding wheel using an image sensor with machine learning techniques. Statistical features, mean, standard deviation, and entropy were selected from the simplified wear model. The selected features were extracted from the wheel surface images of an electroplated cubic Boron Nitride (cBN) wheel on a commercial CNC machine, and compared to the tool condition represented by the number of grinding passes. Finally, the statistical features were fused by machine learning techniques to estimate the wear condition of the grinding wheel. Results show a sufficient correspondence between the estimated and true tool condition with 0.9590 of the coefficient of determination (R2) based on support vector regression model.

加工过程中使用的刀具状态一般由磨损状态决定,磨损状态是影响制造过程加工效率的重要因素。提出了一种利用图像传感器结合机器学习技术对砂轮刀具状态进行估计的方法。从简化的磨损模型中选择统计特征、平均值、标准差和熵。从商用数控机床上电镀立方氮化硼(cBN)砂轮表面图像中提取所选特征,并与磨削道次所代表的刀具状态进行比较。最后,利用机器学习技术对统计特征进行融合,估计砂轮的磨损状况。结果表明,基于支持向量回归模型的估算值与真实刀具状态有充分的对应关系,决定系数(R2)为0.9590。
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引用次数: 4
Evaluation of laser polishing as post-processing of Inconel 625 produced by Directed Energy Deposition 激光抛光作为定向能沉积法生产的Inconel 625后处理的评价
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.06.040
Kandice S.B. Ribeiro , Fábio E. Mariani , Henrique T. Idogava , Gustavo C. da Silva , Zilda C. Silveira , Milton S.F. de Lima , Reginaldo T. Coelho

As one of the Additive Manufacturing (AM) technologies, Directed Energy Deposition (DED) inherited common concerns from the AM concept, such as the layer discretization process, which brings about the staircase effect, and the complex thermal reheating cycles that occur due to the scanning strategies. In this regard, diversification in slicing strategies and the application of post-processing techniques arises as a feasible way to reduce defects and the usual poor surface finishing of DED parts. This study combines the non-planar slicing strategy as toolpath for laser polishing (LP) in DED parts, evaluating the influence of such post-processing for surface finishing, geometry and hardness of the Inconel 625 truncated pyramid built by DED. This geometry was built by using planar slicing and zigzag scanning strategy in BeAM M250 DED equipment. The non-planar coordinates of the LP were applied at an offset in the external profile of the pyramid with support of an open-source software Slic3R. The workpieces were built with laser power of 500 W delivered at the 800 μm spot diameter with scanning rate at 2000 mm/min and powder flow of 7.5 g/min. Laser polishing was performed considering three levels of energy density (9.38, 11.25 and 14.06 J/mm2) and the number of iterations (once, 3 and 5 times). Both, deposition and LP, were performed with local Argon gas shielding. Among the results, LP has shown to be a feasible post-processing technique to L-DED parts, which can improve surface finishing and shape, maintaining hardness. The energy spent LP causes the remelting of satellite particles and shown to be not enough to rearrange the microstructure. The non-planar slicing applied, as toolpath to the LP, comprises a new possibility allowing DED parts to have better results towards surface finishing with a clean and quick method of post-processing. This has shown to be beneficial to the efficiency of the post-processing stage by leading to a greater assertiveness of the process, less post-processing time and no waste of material.

作为增材制造(AM)技术的一种,定向能沉积(DED)继承了增材制造概念的共同问题,如层离散化过程带来的阶梯效应,以及由于扫描策略而产生的复杂的热再加热循环。在这方面,多样化的切片策略和后处理技术的应用成为减少缺陷和DED零件通常较差的表面精加工的可行方法。本研究将非平面切片策略作为激光抛光(LP)零件的刀具路径,评估了这种后处理对激光抛光制造的Inconel 625削尖锥体表面精加工、几何形状和硬度的影响。在BeAM M250 DED设备上采用平面切片和之字形扫描策略建立了该几何结构。在开源软件Slic3R的支持下,将LP的非平面坐标应用于金字塔外部轮廓的偏移处。激光功率为500 W,光斑直径为800 μm,扫描速度为2000 mm/min,粉末流量为7.5 g/min。激光抛光的能量密度分别为9.38、11.25和14.06 J/mm2,迭代次数分别为1次、3次和5次。沉积和LP均在局部氩气保护下进行。结果表明,LP是一种可行的后处理技术,可以改善表面光洁度和形状,保持硬度。LP消耗的能量导致卫星粒子重熔,但不足以重新排列微观结构。应用非平面切片作为LP的刀具路径,包含了一种新的可能性,允许DED部件通过清洁和快速的后处理方法获得更好的表面精加工结果。这已被证明有利于提高后处理阶段的效率,因为这会使处理过程更加自信,减少后处理时间,并且不会浪费材料。
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引用次数: 2
Surface extraction from micro-computed tomography data for additive manufacturing 用于增材制造的微计算机断层扫描数据表面提取
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.06.057
Weijun Shen , Xiao Zhang , Xuepeng Jiang , Li-Hsin Yeh , Zhan Zhang , Qing Li , Beiwen Li , Hantang Qin

Surface topography and surface finish are two significant factors for evaluating the quality and dimensional accuracy of additive manufacturing (AM) parts. In general, compared with traditional subtraction and forming manufacturing techniques, the nature of the rough surface and the geometric complexity make the surface of AM parts "another surface," and traditional methods such as coordinate machine measurement may not be applicable. Most research on surface extraction focuses on regular surfaces, such as flat, cylindrical, or spherical surfaces, but less has been done with irregular surfaces. This paper presented an approach for extracting irregular surfaces based on micro-computed tomography (μ-CT) data of AM parts. The extracted data sets were then compared with data sets obtained by a structured light system (SLS). Areal surface texture parameters, cloud comparison methods, and statistical methods were applied to evaluate the difference between the surface data obtained by the two systems. The results showed that the two surface data correlated well and confirmed the capability of the proposed method for surface extraction from irregular surfaces. This work will contribute to the further multi-sensor data fusion for metrology and provide valuable information for near-surface defects prediction with real-time in-situ metrology method in additive manufacturing.

表面形貌和表面光洁度是评价增材制造(AM)零件质量和尺寸精度的两个重要因素。总的来说,与传统的减法和成形制造技术相比,粗糙表面的性质和几何复杂性使增材制造零件的表面成为“另一个表面”,传统的坐标机测量等方法可能不适用。大多数表面提取的研究集中在规则表面,如平面、圆柱或球面,但对不规则表面的研究较少。提出了一种基于增材制造零件微计算机断层扫描(μ-CT)数据的不规则表面提取方法。然后将提取的数据集与结构光系统(SLS)获得的数据集进行比较。采用面状表面纹理参数、云比较法和统计学方法对两种系统获得的表面数据进行差异性评价。结果表明,两种表面数据相关性良好,证实了该方法对不规则表面的提取能力。这项工作将有助于进一步的多传感器数据融合计量,并为增材制造中近表面缺陷的实时原位测量方法预测提供有价值的信息。
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引用次数: 0
Applying Task-Oriented Safety Field Calibration in Human Robot Collaborative Systems 面向任务的安全现场标定在人机协作系统中的应用
Pub Date : 2021-01-01 DOI: 10.1016/j.promfg.2021.06.067
Cheng Zhu, Tian Yu, Qing Chang

Effective and safe human-robot collaboration (HRC) in assembly requires a reasonable proximity between humans and robots. This paper considers an assembly scenario that humans and robots collaborate in a shared workspace, where safety is the most important issue. A dynamic safety field is defined for HRC to ensure safe collaboration and interaction between a human operator and a robot to achieve assembly tasks. The safety field can be adjusted and calibrated based on predicted assembly tasks. Utilizing the dynamically calibrated safety field, the robot movement will be obtained. The dynamic calibration of safety field for robot movement presents an alternative easier way for robot motion planning based on safety sense. Case studies are performed to demonstrate the effectiveness of the proposed dynamic safety field motion planning method.

在装配过程中,高效、安全的人机协作(HRC)要求人与机器人之间保持合理的距离。本文考虑了人类和机器人在共享工作空间中协作的装配场景,其中安全是最重要的问题。定义了HRC动态安全域,以确保操作人员和机器人之间安全协作和交互,以完成装配任务。安全场可以根据预测的装配任务进行调整和校准。利用动态标定的安全场,得到机器人的运动轨迹。机器人运动安全场的动态标定为基于安全感的机器人运动规划提供了一种更为简便的方法。通过实例分析,验证了所提出的动态安全场运动规划方法的有效性。
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引用次数: 0
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Procedia manufacturing
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