It is widely accepted that old-aged forest can accumulate soil organic carbon (SOC). How microbial physiological traits respond to forest age and whether they drive SOC sequestration in old-aged forest remain elusive. Therefore, we compared the microbial C use efficiency (CUE), biomass turnover rate (rB), microbial biomass C (MBC) and necromass C (MNC) across soil profiles from middle and old-aged forest and evaluated how these microbial traits are related to SOC storage. The results revealed that both forests could accumulate SOC and old-aged forest supported higher SOC storage than middle-aged forest from 2005 to 2020. Moreover, SOC was concentrated on the surface soils of middle-aged forest, whereas it was more distributed across the deeper soil profile in old-aged forest. Compared with middle-aged forest, the O, A and B soil layers of old-aged forest presented increases in microbial CUE (17.8%, 36.9% and 25.0%, respectively), rB (43.7%, 39.7% and 10.8%, respectively), MBC (114.8%, 81.1% and 122.9%, respectively), and MNC content (47.0%, 22.2% and 21.6%, respectively). Random forest analysis suggested that SOC accumulation is controlled mainly by microbial physiological traits rather than other factors including environmental variables. Specifically, microbial CUE and turnover rates increased in old-aged forest, resulting in higher MBC and MNC contents, which in turn led to SOC accumulation. Moreover, the effects of plant and soil properties on SOC storage are regulated mainly by microbial-physiological parameters and the size of microbial C pools. Our findings provide valuable insights into the microbial mechanisms underlying SOC storage in old-aged forest.
{"title":"Increased microbial carbon use efficiency and turnover rate drive soil organic carbon storage in old-aged forest on the southeastern Tibetan Plateau","authors":"Shenglan Ma, Wanze Zhu, Wenwu Wang, Xia Li, Zheliang Sheng, Wolfgang Wanek","doi":"10.1007/s00374-024-01877-y","DOIUrl":"https://doi.org/10.1007/s00374-024-01877-y","url":null,"abstract":"<p>It is widely accepted that old-aged forest can accumulate soil organic carbon (SOC). How microbial physiological traits respond to forest age and whether they drive SOC sequestration in old-aged forest remain elusive. Therefore, we compared the microbial C use efficiency (CUE), biomass turnover rate (rB), microbial biomass C (MBC) and necromass C (MNC) across soil profiles from middle and old-aged forest and evaluated how these microbial traits are related to SOC storage. The results revealed that both forests could accumulate SOC and old-aged forest supported higher SOC storage than middle-aged forest from 2005 to 2020. Moreover, SOC was concentrated on the surface soils of middle-aged forest, whereas it was more distributed across the deeper soil profile in old-aged forest. Compared with middle-aged forest, the O, A and B soil layers of old-aged forest presented increases in microbial CUE (17.8%, 36.9% and 25.0%, respectively), rB (43.7%, 39.7% and 10.8%, respectively), MBC (114.8%, 81.1% and 122.9%, respectively), and MNC content (47.0%, 22.2% and 21.6%, respectively). Random forest analysis suggested that SOC accumulation is controlled mainly by microbial physiological traits rather than other factors including environmental variables. Specifically, microbial CUE and turnover rates increased in old-aged forest, resulting in higher MBC and MNC contents, which in turn led to SOC accumulation. Moreover, the effects of plant and soil properties on SOC storage are regulated mainly by microbial-physiological parameters and the size of microbial C pools. Our findings provide valuable insights into the microbial mechanisms underlying SOC storage in old-aged forest.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-29DOI: 10.1007/s00374-024-01874-1
Yang Yu, Xia Kang, Tianhai Liu, Yong Wang, Jie Tang, Weihong Peng, Francis M. Martin, Hao Tan
Utilising the rhizosphere microbiota as a biological control agent is a promising strategy to protect plants against pathogens, although its efficacy in fungal hosts is uncertain. This study investigated the efficacy of Pseudomonas chlororaphis, a bacterial strain, in mitigating Paecilomyces penicillatus, a soil-borne pathogenic fungus responsible for white mould disease (WMD) in cultivated morels, such as Morchella importuna. Soils with chronic WMD, inoculated with or without P. chlororaphis, were utilised for M. importuna cultivation. In P. chlororaphis-inoculated morel soil beds, P. chlororaphis colonised both the mycelial surface and ascocarp matrix of M. importuna, increasing the abundance of Morchella in soil and the α-diversity of the soil fungal community. Additionally, P. chlororaphis inoculation decreased the abundance of detrimental P. penicillatus and mitigated the WMD incidence, which correspondingly increased the morel yield. Metagenomics revealed that increasing the pseudomonads in the M. importuna mycosphere altered the functionalities of the M. importuna soil microbiota, enhancing the abundances of genes encoding chitinase and alkaline protease and reducing the abundances of genes encoding glucanase and laccase. Under P. chlororaphis inoculation, pathways associated with pathogenic invasion were under-represented in the soil microbiota. These results enhance our understanding of bacterial–fungal interactions within soil ecosystems and demonstrate the potential for disease suppression through microbiota manipulation within the fungal mycosphere. These insights may lead to innovative approaches to combat fungal pathogens and enhance the health and productivity of valuable fungal crops such as morels.
利用根瘤微生物群作为生物防治剂是保护植物免受病原体侵害的一种很有前景的策略,但其对真菌宿主的功效尚不确定。本研究调查了绿假丝酵母菌(一种细菌菌株)在减轻青霉酵母菌(Paecilomyces penicillatus)方面的功效,青霉酵母菌是一种土传致病真菌,是导致栽培羊肚菌(如 Mochella importuna)白霉病(WMD)的罪魁祸首。有慢性 WMD 的土壤在接种或未接种 P. chlororaphis 的情况下被用来栽培 M. importuna。在接种了 P. chlororaphis 的羊肚菌土壤床中,P. chlororaphis 在 M. importuna 的菌丝表面和 ascocarp 基质上都有定殖,增加了土壤中 Morchella 的数量和土壤真菌群落的 α-多样性。此外,接种 P. chlororaphis 减少了有害的 P. penicillatus 的数量,降低了 WMD 的发生率,从而相应地提高了羊肚菌的产量。元基因组学显示,增加进口羊肚菌菌丝圈中的假单胞菌改变了进口羊肚菌土壤微生物群的功能,提高了几丁质酶和碱性蛋白酶编码基因的丰度,降低了葡聚糖酶和漆酶编码基因的丰度。在 P. chlororaphis 接种的情况下,与病原体入侵相关的途径在土壤微生物群中的代表性不足。这些结果加深了我们对土壤生态系统中细菌-真菌相互作用的理解,并证明了通过操纵真菌菌丝圈中的微生物群抑制疾病的潜力。这些见解可能会带来创新的方法来对抗真菌病原体,并提高羊肚菌等珍贵真菌作物的健康和产量。
{"title":"Inoculation of the Morchella importuna mycosphere with Pseudomonas chlororaphis alleviated a soil-borne disease caused by Paecilomyces penicillatus","authors":"Yang Yu, Xia Kang, Tianhai Liu, Yong Wang, Jie Tang, Weihong Peng, Francis M. Martin, Hao Tan","doi":"10.1007/s00374-024-01874-1","DOIUrl":"https://doi.org/10.1007/s00374-024-01874-1","url":null,"abstract":"<p>Utilising the rhizosphere microbiota as a biological control agent is a promising strategy to protect plants against pathogens, although its efficacy in fungal hosts is uncertain. This study investigated the efficacy of <i>Pseudomonas chlororaphis</i>, a bacterial strain, in mitigating <i>Paecilomyces penicillatus</i>, a soil-borne pathogenic fungus responsible for white mould disease (WMD) in cultivated morels, such as <i>Morchella importuna</i>. Soils with chronic WMD, inoculated with or without <i>P. chlororaphis</i>, were utilised for <i>M. importuna</i> cultivation. In <i>P. chlororaphis</i>-inoculated morel soil beds, <i>P. chlororaphis</i> colonised both the mycelial surface and ascocarp matrix of <i>M. importuna</i>, increasing the abundance of <i>Morchella</i> in soil and the α-diversity of the soil fungal community. Additionally, <i>P. chlororaphis</i> inoculation decreased the abundance of detrimental <i>P. penicillatus</i> and mitigated the WMD incidence, which correspondingly increased the morel yield. Metagenomics revealed that increasing the pseudomonads in the <i>M. importuna</i> mycosphere altered the functionalities of the <i>M. importuna</i> soil microbiota, enhancing the abundances of genes encoding chitinase and alkaline protease and reducing the abundances of genes encoding glucanase and laccase. Under <i>P. chlororaphis</i> inoculation, pathways associated with pathogenic invasion were under-represented in the soil microbiota. These results enhance our understanding of bacterial–fungal interactions within soil ecosystems and demonstrate the potential for disease suppression through microbiota manipulation within the fungal mycosphere. These insights may lead to innovative approaches to combat fungal pathogens and enhance the health and productivity of valuable fungal crops such as morels.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142536597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-25DOI: 10.1007/s00374-024-01876-z
Etelvino Henrique Novotny, Eduardo Ribeiro de Azevedo, Jie Wang, Evan McCarney, Petrik Galvosas
The dissolution of fertilisers is the initial process that takes place in soils following fertiliser application and influences the fate and effectiveness of fertilisers. Currently, there are only a few methods for studying fertiliser dissolution in soil. These approaches typically do not accurately represent real soil-fertiliser systems and are susceptible to errors, since they are influenced by processes associated with the loss or retention of the trace ions of the fertiliser. Low field NMR or time-domain NMR (1H-TDNMR) is typically employed for studying 1H in fluids (or mobile 1H), however, special pulse sequences enable the selective detection of 1H in solids. Furthermore, it is possible to filter out undesired signals like 1H from minerals and from soil organic matter. This allows for the detection and monitoring of 1H only from protonated fertilisers (e.g., ammonia, (di)-hydrogen phosphates, etc.). The aim of this study is to present an efficient procedure which monitors the dissolution of fertilisers in soils using 1H-TDNMR. For this, six contrasting New Zealand soils and four protonated fertilisers - NH4Cl, NH4NO3, NaH2PO4.H2O, and (NH4)2HPO4 - were utilised. The proposed method efficiently, accurately, and precisely, monitored the dissolution of the studied fertilisers in all the tested soils under different rain regimes, from violent rain (60 mm h− 1) to light rain (2 mm h− 1) with a time interval (temporal resolution) as short as 5 s.
{"title":"Solid-state nuclear magnetic resonance at low-field as an approach for fertiliser dissolution monitoring","authors":"Etelvino Henrique Novotny, Eduardo Ribeiro de Azevedo, Jie Wang, Evan McCarney, Petrik Galvosas","doi":"10.1007/s00374-024-01876-z","DOIUrl":"https://doi.org/10.1007/s00374-024-01876-z","url":null,"abstract":"<p>The dissolution of fertilisers is the initial process that takes place in soils following fertiliser application and influences the fate and effectiveness of fertilisers. Currently, there are only a few methods for studying fertiliser dissolution in soil. These approaches typically do not accurately represent real soil-fertiliser systems and are susceptible to errors, since they are influenced by processes associated with the loss or retention of the trace ions of the fertiliser. Low field NMR or time-domain NMR (<sup>1</sup>H-TDNMR) is typically employed for studying <sup>1</sup>H in fluids (or mobile <sup>1</sup>H), however, special pulse sequences enable the selective detection of <sup>1</sup>H in solids. Furthermore, it is possible to filter out undesired signals like <sup>1</sup>H from minerals and from soil organic matter. This allows for the detection and monitoring of <sup>1</sup>H only from protonated fertilisers (e.g., ammonia, (di)-hydrogen phosphates, etc.). The aim of this study is to present an efficient procedure which monitors the dissolution of fertilisers in soils using <sup>1</sup>H-TDNMR. For this, six contrasting New Zealand soils and four protonated fertilisers - NH<sub>4</sub>Cl, NH<sub>4</sub>NO<sub>3</sub>, NaH<sub>2</sub>PO<sub>4</sub>.H<sub>2</sub>O, and (NH<sub>4</sub>)<sub>2</sub>HPO<sub>4</sub> - were utilised. The proposed method efficiently, accurately, and precisely, monitored the dissolution of the studied fertilisers in all the tested soils under different rain regimes, from violent rain (60 mm h<sup>− 1</sup>) to light rain (2 mm h<sup>− 1</sup>) with a time interval (temporal resolution) as short as 5 s.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142489471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1007/s00374-024-01873-2
Marine Papin, Amélie Polrot, Marie-Christine Breuil, Sonia Czarnes, Assia Dreux-Zigha, Xavier Le Roux, Ahmed Taibi, Aymé Spor, Laurent Philippot
Despite the rapid development of microbial inoculants use, their effectiveness still lacks robustness, partly due to our limited understanding of the factors influencing their establishment in soil. Recurrent inoculation can temporarily increase their abundance, but the effect of this inoculation strategy on plant growth and on the resident microbial community is still poorly studied. Here, we investigated maize growth and soil bacterial community responses under recurrent inoculation of the plant-beneficial bacterium Pseudomonas fluorescens B177. We further assessed how the effect of recurrent inoculation was modulated by the inoculant dose, the application timing and the soil type. Recurrent inoculation at high dose transiently increased the abundance of P. fluorescens B177 and resulted in larger shifts in the resident bacterial community compared to a single inoculation event. Moreover, recurrent inoculation prior to sowing had the strongest effect on maize growth, with increased shoot dry weight by 47.4%, likely due to an indirect effect of the inoculant through early changes in the resident community. Altogether these findings highlight the significance of recurrent pre-sowing inoculations as an alternative strategy for promoting plant growth.
{"title":"Pre-sowing recurrent inoculation with Pseudomonas fluorescens promotes maize growth","authors":"Marine Papin, Amélie Polrot, Marie-Christine Breuil, Sonia Czarnes, Assia Dreux-Zigha, Xavier Le Roux, Ahmed Taibi, Aymé Spor, Laurent Philippot","doi":"10.1007/s00374-024-01873-2","DOIUrl":"https://doi.org/10.1007/s00374-024-01873-2","url":null,"abstract":"<p>Despite the rapid development of microbial inoculants use, their effectiveness still lacks robustness, partly due to our limited understanding of the factors influencing their establishment in soil. Recurrent inoculation can temporarily increase their abundance, but the effect of this inoculation strategy on plant growth and on the resident microbial community is still poorly studied. Here, we investigated maize growth and soil bacterial community responses under recurrent inoculation of the plant-beneficial bacterium <i>Pseudomonas fluorescens</i> B177. We further assessed how the effect of recurrent inoculation was modulated by the inoculant dose, the application timing and the soil type. Recurrent inoculation at high dose transiently increased the abundance of <i>P. fluorescens</i> B177 and resulted in larger shifts in the resident bacterial community compared to a single inoculation event. Moreover, recurrent inoculation prior to sowing had the strongest effect on maize growth, with increased shoot dry weight by 47.4%, likely due to an indirect effect of the inoculant through early changes in the resident community. Altogether these findings highlight the significance of recurrent pre-sowing inoculations as an alternative strategy for promoting plant growth.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-22DOI: 10.1007/s00374-024-01875-0
Shoujia Zhuo, Yunying Fang, Youchao Chen, Tony Vancov, Huaqiang Du, Yongfu Li, Bing Yu, Scott X. Chang, Yanjiang Cai
The impact of plant litter on soil carbon (C) cycling is influenced by external nitrogen (N) deposition and plant litter chemistry. While previous research has mainly focused on inorganic N deposition and its effect on plant litter decomposition and soil C cycling, the influence of organic N remains poorly understood. In this study, we conducted a 180-day incubation experiment to investigate how different N forms (NH4NO3, Urea 50% + Glycine 50%) and litter chemistry (varying lignin/N ratios) affect CO2 emissions from an acidic Moso bamboo (Phyllostachys edulis) forest soil. Our findings indicate that litter addition increased soil CO2 emissions and the proportion of CO2-C to Total C (considering added litter-C as a part of total C). Specifically, Moso bamboo leaf litter with a lower lignin/N ratio led to higher soil CO2 emissions and CO2-C/Total C ratios. The combined addition of litter and N exhibited an antagonistic effect on soil CO2 emissions, with inorganic N having a more pronounced effect compared to organic N. This antagonistic effect was attributed to the N addition-induced soil acidification, thereby inhibiting microbial activities and reducing soil respiration promoted by litter input. This effect was confirmed by random forest analysis and partial least squares path modeling, which further identified soil dissolved organic C and pH as critical factors positively influencing soil CO2 emissions. Overall, our study suggests that atmospheric N deposition can mitigate litter-induced soil CO2 emissions, particularly under inorganic N forms and when leaf litters with high lignin/N ratios are introduced.
植物废弃物对土壤碳(C)循环的影响受到外部氮(N)沉积和植物废弃物化学性质的影响。以往的研究主要集中于无机氮沉积及其对植物枯落物分解和土壤碳循环的影响,而对有机氮的影响仍知之甚少。在这项研究中,我们进行了一项为期 180 天的培养实验,以研究不同的氮形式(NH4NO3、50% 尿素 + 50% 甘氨酸)和枯落物化学(不同的木质素/N 比率)如何影响酸性毛竹林土壤的二氧化碳排放。我们的研究结果表明,凋落物的添加增加了土壤的二氧化碳排放量以及二氧化碳-C 占总 C 的比例(将添加的凋落物-C 视为总 C 的一部分)。具体来说,木质素/氮比率较低的毛竹落叶会导致较高的土壤二氧化碳排放量和二氧化碳-碳/总碳比率。这种拮抗作用归因于氮的添加引起了土壤酸化,从而抑制了微生物的活动,降低了因添加枯落物而促进的土壤呼吸作用。随机森林分析和偏最小二乘路径模型证实了这种效应,进一步确定了土壤溶解有机碳和 pH 值是对土壤二氧化碳排放产生积极影响的关键因素。总之,我们的研究表明,大气中的氮沉积可以缓解枯落物引起的土壤二氧化碳排放,特别是在无机氮形式下和引入高木质素/氮比的叶片枯落物时。
{"title":"Interactive effects of plant litter chemistry and organic/inorganic forms of nitrogen addition on Moso bamboo (Phyllostachys edulis) soil respiration","authors":"Shoujia Zhuo, Yunying Fang, Youchao Chen, Tony Vancov, Huaqiang Du, Yongfu Li, Bing Yu, Scott X. Chang, Yanjiang Cai","doi":"10.1007/s00374-024-01875-0","DOIUrl":"https://doi.org/10.1007/s00374-024-01875-0","url":null,"abstract":"<p>The impact of plant litter on soil carbon (C) cycling is influenced by external nitrogen (N) deposition and plant litter chemistry. While previous research has mainly focused on inorganic N deposition and its effect on plant litter decomposition and soil C cycling, the influence of organic N remains poorly understood. In this study, we conducted a 180-day incubation experiment to investigate how different N forms (NH<sub>4</sub>NO<sub>3</sub>, Urea 50% + Glycine 50%) and litter chemistry (varying lignin/N ratios) affect CO<sub>2</sub> emissions from an acidic Moso bamboo (<i>Phyllostachys edulis</i>) forest soil. Our findings indicate that litter addition increased soil CO<sub>2</sub> emissions and the proportion of CO<sub>2</sub>-C to Total C (considering added litter-C as a part of total C). Specifically, Moso bamboo leaf litter with a lower lignin/N ratio led to higher soil CO<sub>2</sub> emissions and CO<sub>2</sub>-C/Total C ratios. The combined addition of litter and N exhibited an antagonistic effect on soil CO<sub>2</sub> emissions, with inorganic N having a more pronounced effect compared to organic N. This antagonistic effect was attributed to the N addition-induced soil acidification, thereby inhibiting microbial activities and reducing soil respiration promoted by litter input. This effect was confirmed by random forest analysis and partial least squares path modeling, which further identified soil dissolved organic C and pH as critical factors positively influencing soil CO<sub>2</sub> emissions. Overall, our study suggests that atmospheric N deposition can mitigate litter-induced soil CO<sub>2</sub> emissions, particularly under inorganic N forms and when leaf litters with high lignin/N ratios are introduced.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142486884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-18DOI: 10.1007/s00374-024-01872-3
Elisa Zampieri, Fabiano Sillo, Giulio Metelli, Maria Alexandra Cucu, Vincenzo Montesano, Giulia Quagliata, Lena Philipp, Francesca Brescia, Adriano Conte, Luca Giovannini, Carmelo Mennone, Angelo Fiore, Stefania Astolfi, Daniel Savatin, Francesco Sestili, Thomas Reitz, Raffaella Balestrini
Intercropping, based on the interplay between cereals and legumes, might be an encouraging approach to improve soil fertility and crop productivity and to guarantee more sustainable farming systems. However, plant consociation is also influenced by the interaction between roots and soil microbial communities, and different plant genotypes might differently respond to this management. Here, a 2-year field study was carried out, verifying the impact of intercropping and the inoculation with arbuscular mycorrhizal fungi (AMF) on two varieties of durum wheat, using a lentil variety as intercropped plant species, on wheat agronomic parameters and grain features, as well as on microbial communities of soil, rhizosphere and wheat roots. Results showed a genotype effect on diverse agronomic parameters, gluten quality and grain elemental concentrations. Additionally, intercropping and AM fungal inoculation affected and shaped the microbial alpha diversity and composition, especially for the AMF community, at root level. Overall, the effects of the considered treatments (intercropping with lentil and AM fungal inoculation) were noticeably influenced by the specific wheat genotype, suggesting the importance to conduct a careful selection of intercropped genotypes.
以谷物和豆科植物之间的相互作用为基础的间作可能是一种令人鼓舞的方法,可以提高土壤肥力和作物生产力,并保证耕作系统更具可持续性。然而,植物的联合也受到根系和土壤微生物群落之间相互作用的影响,不同的植物基因型可能对这种管理方式做出不同的反应。在此,我们进行了一项为期两年的田间研究,验证了间作和接种丛枝菌根真菌(AMF)对两个硬粒小麦品种(使用扁豆品种作为间作植物物种)的影响,以及对小麦农艺参数和谷物特征以及土壤、根瘤菌层和小麦根部微生物群落的影响。结果表明,基因型对各种农艺参数、面筋质量和谷物元素浓度都有影响。此外,间作和AM真菌接种也影响和塑造了微生物α的多样性和组成,特别是根层的AMF群落。总之,所考虑的处理方法(与扁豆间作和接种 AM 真菌)的效果明显受到特定小麦基因型的影响,这表明谨慎选择间作基因型的重要性。
{"title":"Insights into the influence of intercropping and arbuscular mycorrhizal inoculation on two modern durum wheat cultivars and their associated microbiota","authors":"Elisa Zampieri, Fabiano Sillo, Giulio Metelli, Maria Alexandra Cucu, Vincenzo Montesano, Giulia Quagliata, Lena Philipp, Francesca Brescia, Adriano Conte, Luca Giovannini, Carmelo Mennone, Angelo Fiore, Stefania Astolfi, Daniel Savatin, Francesco Sestili, Thomas Reitz, Raffaella Balestrini","doi":"10.1007/s00374-024-01872-3","DOIUrl":"https://doi.org/10.1007/s00374-024-01872-3","url":null,"abstract":"<p>Intercropping, based on the interplay between cereals and legumes, might be an encouraging approach to improve soil fertility and crop productivity and to guarantee more sustainable farming systems. However, plant consociation is also influenced by the interaction between roots and soil microbial communities, and different plant genotypes might differently respond to this management. Here, a 2-year field study was carried out, verifying the impact of intercropping and the inoculation with arbuscular mycorrhizal fungi (AMF) on two varieties of durum wheat, using a lentil variety as intercropped plant species, on wheat agronomic parameters and grain features, as well as on microbial communities of soil, rhizosphere and wheat roots. Results showed a genotype effect on diverse agronomic parameters, gluten quality and grain elemental concentrations. Additionally, intercropping and AM fungal inoculation affected and shaped the microbial alpha diversity and composition, especially for the AMF community, at root level. Overall, the effects of the considered treatments (intercropping with lentil and AM fungal inoculation) were noticeably influenced by the specific wheat genotype, suggesting the importance to conduct a careful selection of intercropped genotypes.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142448385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-14DOI: 10.1007/s00374-024-01871-4
Rubén Martínez-Cuesta, Anna Holmer, Franz Buegger, Michael Dannenmann, Michael Schloter, Stefanie Schulz
Understanding the impact of agricultural land use on the soil prokaryotic communities in connected downslope sites is crucial for developing sustainable strategies to preserve ecosystem properties and mitigate agriculture’s environmental impacts. In this study, we investigated topsoil samples collected at three time points in 2022 (March, June, and November) from two adjacent catenas, reaching from hillslope to floodplain. The catenas differed in land use (extensive grassland vs. extensive cropland) at the top and middle parts, while the floodplain remained an extensive grassland due to legal restrictions. Using quantitative real-time PCRs and metabarcoding, we assessed prokaryotic abundance and prokaryotic community composition. Results show higher bacterial abundance in the cropland-influenced floodplain part across all time points compared to the grassland-influenced floodplain part. Temporal dynamics revealed a progressive decrease in the shared prokaryotic communities of the floodplain parts, peaking at the summer sampling time point, indicating a significant influence of the respective management type of the agricultural sites over the bacterial and archaeal communities of the floodplain parts. Differential abundance analyses identified several nitrifying taxa as more abundant in the cropland-influenced floodplain. Upstream land use also influenced the prokaryotic network of the cropland-floodplain, with some cropland taxa becoming keystone taxa and altering network morphology, an effect not observed in the grassland-influenced floodplain. These findings suggest that upstream agricultural land use practices have exerted a long-term influence on the floodplain prokaryotic communities over the past three decades. Moreover, there is evidence suggesting that these prokaryotic communities may undergo a potential reset during winter, which requires further investigation.
{"title":"Land use drives prokaryotic community composition of directly adjacent grasslands","authors":"Rubén Martínez-Cuesta, Anna Holmer, Franz Buegger, Michael Dannenmann, Michael Schloter, Stefanie Schulz","doi":"10.1007/s00374-024-01871-4","DOIUrl":"https://doi.org/10.1007/s00374-024-01871-4","url":null,"abstract":"<p>Understanding the impact of agricultural land use on the soil prokaryotic communities in connected downslope sites is crucial for developing sustainable strategies to preserve ecosystem properties and mitigate agriculture’s environmental impacts. In this study, we investigated topsoil samples collected at three time points in 2022 (March, June, and November) from two adjacent catenas, reaching from hillslope to floodplain. The catenas differed in land use (extensive grassland vs. extensive cropland) at the top and middle parts, while the floodplain remained an extensive grassland due to legal restrictions. Using quantitative real-time PCRs and metabarcoding, we assessed prokaryotic abundance and prokaryotic community composition. Results show higher bacterial abundance in the cropland-influenced floodplain part across all time points compared to the grassland-influenced floodplain part. Temporal dynamics revealed a progressive decrease in the shared prokaryotic communities of the floodplain parts, peaking at the summer sampling time point, indicating a significant influence of the respective management type of the agricultural sites over the bacterial and archaeal communities of the floodplain parts. Differential abundance analyses identified several nitrifying taxa as more abundant in the cropland-influenced floodplain. Upstream land use also influenced the prokaryotic network of the cropland-floodplain, with some cropland taxa becoming keystone taxa and altering network morphology, an effect not observed in the grassland-influenced floodplain. These findings suggest that upstream agricultural land use practices have exerted a long-term influence on the floodplain prokaryotic communities over the past three decades. Moreover, there is evidence suggesting that these prokaryotic communities may undergo a potential reset during winter, which requires further investigation.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-10DOI: 10.1007/s00374-024-01869-y
Zhihua Bao, Jing Cui, Jumei Liu, Meng Zhang, Linxia Chen, Weiwei Cao, Ke Yu, Lixin Wang, Zhongjun Jia, Ji Zhao
The root-associated type II methanotrophs significantly contribute to CH4 oxidation-dependent N2 fixation. However, it is unclear whether type I methanotrophs are involved in CH4 oxidation and N2 fixation, especially in natural wetlands. So far, limited attention has given to root-associated active microorganisms. Here, metatranscriptomic analysis of root-associated microbes has been proposed to reveal the aerobic methanotrophs contributing to CH4 and nitrogen cycles in the roots of Phragmites australis grown in a natural wetland. Results showed Methylocystaceae (type II methanotrophs) and Methylococcaceae (type I methanotrophs) as major taxa (relative abundance, 14%) at transcription level. However, based on 16S rRNA gene sequencing, contribution of these taxa was < 1% at DNA level. Genes encoding methane monooxygenase (enzyme responsible for the first step of CH4 oxidation) were detected in Methylomonas (pmoCBA) and Methylosinus (mmoXYZCB). Furthermore, genes related to methanol dehydrogenase, formaldehyde dehydrogenase, and formate dehydrogenase were also detected in Methyosinus and Methylomonas, while mcrA gene was observed in Methanospirillum and Methanofollis. Moreover, nitrogenase structural genes, such as nifHDK, were found in Methylosinus (Methylocystaceae) and Methylomonas (Methylococcaceae). Minor nitrogenase genes were detected in Cyanothece, Lyngbya, Pelobacter and Smithella of Cyanobacteriaceae family. In addition, N2 fixing activity of P. australis was determined by analyzing the natural abundance of δ15N from June to August. The N2 fixing activity of P. australis increased in presence of CH4 in root system under 15N-N2 feeding. Metatranscriptomic analysis revealed that not only type II methanotrophs, but also type I methanotrophs oxidize CH4 and fix N2.
与根相关的 II 型甲烷营养体对依赖于 CH4 氧化的 N2 固定有很大贡献。然而,目前还不清楚 I 型甲烷营养体是否参与了 CH4 氧化和 N2 固定,尤其是在自然湿地中。迄今为止,人们对与根相关的活性微生物关注有限。本文通过对根相关微生物的元转录组学分析,揭示了生长在天然湿地中的葭萌植物根系中促进 CH4 和氮循环的需氧甲烷营养体。结果表明,在转录水平上,甲囊菌科(II 型甲烷营养体)和甲球菌科(I 型甲烷营养体)是主要类群(相对丰度为 14%)。然而,根据 16S rRNA 基因测序,这些类群在 DNA 水平上的贡献率为 1%。在甲基单胞菌(pmoCBA)和甲基单胞菌(mmoXYZCB)中检测到了编码甲烷单氧化酶(负责 CH4 氧化第一步的酶)的基因。此外,在甲基单胞菌(Methylosinus)和甲基单胞菌(Methylomonas)中还检测到与甲醇脱氢酶、甲醛脱氢酶和甲酸脱氢酶有关的基因,而在甲烷螺旋体(Methanospirillum)和甲烷磷脂菌(Methanofollis)中则观察到 mcrA 基因。此外,在甲基单胞菌(Methylocystaceae)和甲基单胞菌(Methylococcaceae)中发现了氮酶结构基因,如 nifHDK。在蓝藻科的 Cyanothece、Lyngbya、Pelobacter 和 Smithella 中发现了次要的氮酶基因。此外,通过分析 6 月至 8 月δ15N 的自然丰度,确定了 P. australis 的 N2 固定活性。在15N-N2喂养条件下,当根系中存在CH4时,奥氏囊藻的N2固定活性增加。转录组分析表明,不仅 II 型甲烷营养体,I 型甲烷营养体也氧化 CH4 并固定 N2。
{"title":"Metatranscriptomic analysis to reveal the coupling between nitrogen fixation and CH4 oxidation in root tissues of Phragmites australis","authors":"Zhihua Bao, Jing Cui, Jumei Liu, Meng Zhang, Linxia Chen, Weiwei Cao, Ke Yu, Lixin Wang, Zhongjun Jia, Ji Zhao","doi":"10.1007/s00374-024-01869-y","DOIUrl":"https://doi.org/10.1007/s00374-024-01869-y","url":null,"abstract":"<p>The root-associated type II methanotrophs significantly contribute to CH<sub>4</sub> oxidation-dependent N<sub>2</sub> fixation. However, it is unclear whether type I methanotrophs are involved in CH<sub>4</sub> oxidation and N<sub>2</sub> fixation, especially in natural wetlands. So far, limited attention has given to root-associated active microorganisms. Here, metatranscriptomic analysis of root-associated microbes has been proposed to reveal the aerobic methanotrophs contributing to CH<sub>4</sub> and nitrogen cycles in the roots of <i>Phragmites australis</i> grown in a natural wetland. Results showed Methylocystaceae (type II methanotrophs) and Methylococcaceae (type I methanotrophs) as major taxa (relative abundance, 14%) at transcription level. However, based on 16S rRNA gene sequencing, contribution of these taxa was < 1% at DNA level. Genes encoding methane monooxygenase (enzyme responsible for the first step of CH<sub>4</sub> oxidation) were detected in <i>Methylomonas</i> (<i>pmoCBA</i>) and <i>Methylosinus</i> (<i>mmoXYZCB</i>). Furthermore, genes related to methanol dehydrogenase, formaldehyde dehydrogenase, and formate dehydrogenase were also detected in <i>Methyosinus</i> and <i>Methylomonas</i>, while <i>mcrA</i> gene was observed in <i>Methanospirillum</i> and <i>Methanofollis</i>. Moreover, nitrogenase structural genes, such as <i>nifHDK,</i> were found in <i>Methylosinus</i> (Methylocystaceae) and <i>Methylomonas</i> (Methylococcaceae). Minor nitrogenase genes were detected in <i>Cyanothece</i>, <i>Lyngbya</i>, <i>Pelobacter</i> and <i>Smithella</i> of Cyanobacteriaceae family. In addition, N<sub>2</sub> fixing activity of <i>P. australis</i> was determined by analyzing the natural abundance of δ<sup>15</sup>N from June to August. The N<sub>2</sub> fixing activity of <i>P. australis</i> increased in presence of CH<sub>4</sub> in root system under <sup>15</sup>N-N<sub>2</sub> feeding. Metatranscriptomic analysis revealed that not only type II methanotrophs, but also type I methanotrophs oxidize CH<sub>4</sub> and fix N<sub>2</sub>.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-09DOI: 10.1007/s00374-024-01870-5
Luz de-Bashan, Juan D. Giraldo, Mauricio Cruz-Barrera, Mauricio Schoebitz
In the absence of an appropriate formulation, the population of plant growth-promoting bacteria (PGPB) inoculated into soil may be significantly reduced. These unprotected introduced bacteria must compete with the often-more adapted native microflora and are susceptible to predation by soil microfauna. This opinion paper addresses the significance of proper formulation in creating an effective inoculant, discusses the primary challenges associated with current liquid and dry formulations, and emphasizes the rationale for bioencapsulation as the optimal approach for protecting PGPB in a successful inoculant.
{"title":"Enhancing the survival rate and effectiveness of plant growth-promoting bacteria through bioencapsulation techniques","authors":"Luz de-Bashan, Juan D. Giraldo, Mauricio Cruz-Barrera, Mauricio Schoebitz","doi":"10.1007/s00374-024-01870-5","DOIUrl":"https://doi.org/10.1007/s00374-024-01870-5","url":null,"abstract":"<p>In the absence of an appropriate formulation, the population of plant growth-promoting bacteria (PGPB) inoculated into soil may be significantly reduced. These unprotected introduced bacteria must compete with the often-more adapted native microflora and are susceptible to predation by soil microfauna. This opinion paper addresses the significance of proper formulation in creating an effective inoculant, discusses the primary challenges associated with current liquid and dry formulations, and emphasizes the rationale for bioencapsulation as the optimal approach for protecting PGPB in a successful inoculant.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-09DOI: 10.1007/s00374-024-01868-z
Guozhen Gao, Haiyan Cui, Pengfa Li, Shiyu Ma, Ming Liu, Meng Wu, Zhongpei Li
Using two kinds of microbial inoculations extracted from soil cropped to rice and peanut, we conducted a swap-inoculation experiment to explore the relative importance of microbial inoculation and soil properties on CO2 emissions from soil. Inoculated microorganisms into a soil different from their origin (swap inoculation) were partially successful and reduced CO2 emissions, namely according to home-field advantage (HFA); The success of invasive microorganisms depended on molecular composition of soil organic matter (SOM) compared to inoculation of native microbes (inoculated microorganisms into origin soil). The different habits screened the fewer microorganisms to undergo respiration for energy and life-sustaining activities, thus decreasing CO2 emissions from SOM. However, the effect of HFA diminished with incubation time, as the invasive microorganisms reshaped SOM molecular diversity and composition during microbial community assemblage, which fits with the Gaia effect (GE). Specific microbial communities, such as Bacteroidetes and Actinobacteria, drove the conversion of persistent molecules to labile molecules, thereby increasing the chances of SOM mineralization by microorganisms. We found there was positive correlation between labile SOM molecules and SOM mineralization. In addition, MBC increased in swap inoculation compared to native inoculation after 60 days, which also resulted in higher CO2 emissions from SOM. HFA and GE provide new perspectives to help decipher the interaction between microorganisms and the habitat under microbial invasion, and the mechanism of influence on CO2 emissions from SOM.
利用从种植水稻和花生的土壤中提取的两种微生物接种,我们进行了交换接种实验,以探索微生物接种和土壤特性对土壤二氧化碳排放的相对重要性。将微生物接种到与其原产地不同的土壤中(交换接种)获得了部分成功,并减少了二氧化碳排放量,即根据主场优势(HFA);与接种本地微生物(将微生物接种到原产地土壤中)相比,入侵微生物的成功取决于土壤有机质(SOM)的分子组成。不同的生活习性筛选出较少的微生物进行呼吸作用以获取能量和维持生命活动,从而减少了 SOM 的二氧化碳排放量。然而,随着培养时间的延长,HFA 的效果逐渐减弱,因为在微生物群落组合过程中,入侵微生物重塑了 SOM 的分子多样性和组成,这与盖亚效应(GE)相吻合。特定的微生物群落(如类杆菌和放线菌)推动了持久性分子向易变性分子的转化,从而增加了微生物将SOM矿化的机会。我们发现,易变 SOM 分子与 SOM 矿化之间存在正相关。此外,与原生接种相比,交换接种的 MBC 在 60 天后有所增加,这也导致 SOM 的二氧化碳排放量增加。HFA和GE提供了新的视角,有助于解读微生物入侵下微生物与栖息地之间的相互作用,以及对SOM二氧化碳排放的影响机制。
{"title":"Enhanced CO2 emissions from soil organic matter in agricultural fields during microbial community assemblage","authors":"Guozhen Gao, Haiyan Cui, Pengfa Li, Shiyu Ma, Ming Liu, Meng Wu, Zhongpei Li","doi":"10.1007/s00374-024-01868-z","DOIUrl":"https://doi.org/10.1007/s00374-024-01868-z","url":null,"abstract":"<p>Using two kinds of microbial inoculations extracted from soil cropped to rice and peanut, we conducted a swap-inoculation experiment to explore the relative importance of microbial inoculation and soil properties on CO<sub>2</sub> emissions from soil. Inoculated microorganisms into a soil different from their origin (swap inoculation) were partially successful and reduced CO<sub>2</sub> emissions, namely according to home-field advantage (HFA); The success of invasive microorganisms depended on molecular composition of soil organic matter (SOM) compared to inoculation of native microbes (inoculated microorganisms into origin soil). The different habits screened the fewer microorganisms to undergo respiration for energy and life-sustaining activities, thus decreasing CO<sub>2</sub> emissions from SOM. However, the effect of HFA diminished with incubation time, as the invasive microorganisms reshaped SOM molecular diversity and composition during microbial community assemblage, which fits with the Gaia effect (GE). Specific microbial communities, such as <i>Bacteroidetes</i> and <i>Actinobacteria</i>, drove the conversion of persistent molecules to labile molecules, thereby increasing the chances of SOM mineralization by microorganisms. We found there was positive correlation between labile SOM molecules and SOM mineralization. In addition, MBC increased in swap inoculation compared to native inoculation after 60 days, which also resulted in higher CO<sub>2</sub> emissions from SOM. HFA and GE provide new perspectives to help decipher the interaction between microorganisms and the habitat under microbial invasion, and the mechanism of influence on CO<sub>2</sub> emissions from SOM.</p>","PeriodicalId":9210,"journal":{"name":"Biology and Fertility of Soils","volume":null,"pages":null},"PeriodicalIF":6.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142385495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}