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INSULIN RESISTANCE FROM A PERSPECTIVE: A REVIEW 从一个角度看胰岛素抵抗
Pub Date : 2022-12-31 DOI: 10.56557/joban/2022/v14i28039
N. Johnkennedy, Nwaokoro Joakin Chidozie, M. Akram, Nwanguma Eberechi, Azuike Chioma Gladys
The pancreas secretes the hormone insulin, which facilitates the entry of blood glucose into the liver, muscle, and fat cells where it is converted to cellular energy. Indeed, one consumes food to get glucose. When one is fasting or otherwise in need, the liver can also produce glucose. The pancreas produces insulin into the blood when blood glucose levels, also known as blood sugar levels, increase after eating. After then, insulin reduces blood glucose to maintain it within the usual range. When cells in the muscles, fat, and liver do not respond to insulin well and can not utilise glucose from the blood for energy, this condition is known as insulin resistance. The pancreas produces more insulin to make up for the loss. The blood sugar levels rise over time. Obesity, high blood pressure, high cholesterol, and type 2 diabetes are just a few of the issues that are part of the insulin resistance syndrome.
胰腺分泌胰岛素,促进血糖进入肝脏、肌肉和脂肪细胞,在那里转化为细胞能量。事实上,一个人通过进食来获取葡萄糖。当一个人禁食或有其他需要时,肝脏也会产生葡萄糖。当血糖水平(也称为血糖水平)在进食后升高时,胰腺会向血液中产生胰岛素。之后,胰岛素会降低血糖,使其保持在正常范围内。当肌肉、脂肪和肝脏中的细胞对胰岛素反应不佳,无法利用血液中的葡萄糖获取能量时,这种情况被称为胰岛素抵抗。胰腺产生更多的胰岛素来弥补损失。血糖水平会随着时间的推移而升高。肥胖、高血压、高胆固醇和2型糖尿病只是胰岛素抵抗综合征的一部分。
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引用次数: 0
KNOWLEDGE EVOLUTION IN NANOCHITOSAN RESEARCH AND INNOVATIVE BIOTECHNOLOGICAL APPLICATIONS: A MULTINATIONAL ASSESSMENT FOR NOWCAST AND FUTURE FORECAST 纳米壳聚糖研究和创新生物技术应用的知识演变:近期预测和未来预测的多国评估
Pub Date : 2022-12-30 DOI: 10.56557/joban/2022/v14i28016
T. Ekundayo, B. Igere
There had been a growing global need for biomolecular agents due to its diversity in biotechnological application especially chitosan and its derivatives. Current advances in nano-particulate technologies have also abetted the use of biomolecular-based nano-type delivery and functions of chitosan resulting nano-based chitosan/nanochitosan particle. Although there exist notable advances in the exploration and innovative nanochitosan application, future expansion remains bleary. The current study access the knowledge evolution in nanochitosan research and innovative biotechnological applications via science mapping of previous studies from authors, institutions, countries, collaboration networks, and productivity. Retrieved documents were 5,923 with 13,327 authors, from Web of Science (WoS) Core Collection and timespan of 1990 – 2019 using the search title; (Chitosan and nanoparticle*). Our result reveals an increase in the publication of related documents in fifth yearly order. This notable/significant increase observed in subsequent years was revealed by the Lotka’s model with an annual growth rate of 33.32%; Lotka's model beta=1.927; C=0.286; R2=0.89; p.value=0.05. Corresponding Author's Countries were China 1756 (29.65), India 683 (11.53), Japan 47 (0.79); while country citation are; China (51720), India (17595), Korea (10688), Australia (1679). The last decade reports reveal more publications on nanochitosan studies ranging from 238 publications to greater than 746 publications. Although such increase has revealed global known capabilities, research based and/or application of the nanobased-chitosan, there is need for pertinent exploitation of nanochitosan potentials, documentation, application, progresses and future of related studies. A suggestive continuous application of such progresses on chitosan knowledge-research based documentation/exploration has a part with global future research and biotechnological application.
由于生物分子制剂在生物技术应用中的多样性,特别是壳聚糖及其衍生物,全球对生物分子制剂的需求日益增长。纳米颗粒技术的进步也促进了基于生物分子的纳米型壳聚糖的使用和功能,产生了纳米基壳聚糖/纳米壳聚糖颗粒。虽然在纳米壳聚糖的探索和创新应用方面取得了显著进展,但未来的扩展仍然模糊不清。本研究从作者、机构、国家、合作网络和生产力等方面对纳米壳聚糖研究和创新生物技术应用的知识演变进行了科学梳理。检索文献5,923篇,作者13,327名,来自Web of Science (WoS)核心馆藏,使用检索标题检索时间跨度为1990 - 2019;(壳聚糖和纳米颗粒*)。我们的结果显示,相关文件的出版以每年第五次的顺序增加。Lotka模型揭示了随后几年观察到的显著增长,年增长率为33.32%;Lotka模型beta=1.927;C = 0.286;R2 = 0.89;p.value = 0.05。通讯作者国家:中国1756(29.65),印度683(11.53),日本47 (0.79);而国家引用是;中国(51720),印度(17595),韩国(10688),澳大利亚(1679)。过去十年的报告揭示了更多关于纳米壳聚糖研究的出版物,从238份出版物到超过746份出版物。尽管纳米壳聚糖的发展揭示了其全球已知的能力、研究基础和/或应用,但仍需要有针对性地开发纳米壳聚糖的潜力、文献、应用、进展和相关研究的未来。这些研究进展在壳聚糖知识研究文献/探索方面的持续应用,对全球未来的研究和生物技术应用具有重要意义。
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引用次数: 0
THE ROLE OF CHAMPION AND VETERAN TREES IN THE ECOTOURISM OF THE NORTH-WESTERN REGION OF AZERBAIJAN 冠军树和老树在阿塞拜疆西北地区生态旅游中的作用
Pub Date : 2022-12-16 DOI: 10.56557/joban/2022/v14i27979
G. Nazarova
The presented article talks about champion and veteran trees located in the northwest of the Republic of Azerbaijan. These trees are very important for the ecological environment in the world. The article examines the significance of the named trees for the ecology of the Sheki-Zagatala, as well as ecological tourism, and offers suggestions for its progress.
本文介绍了位于阿塞拜疆共和国西北部的冠军树和老树。这些树木对世界生态环境非常重要。本文探讨了命名树木对谢基扎加塔拉生态和生态旅游的意义,并对其发展提出了建议。
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引用次数: 0
SOMATIC EMBRYOGENESIS AND PLANTLET FORMATION FROM LEAF EXPLANTS OF SPONGE GOURD (Luffa aegyptiaca (MILL.) A MEDICINALLY IMPORTANT PLANT 丝瓜(Luffa aegyptiaca, MILL.)叶片外植体体细胞胚胎发生及植株形成重要的药用植物
Pub Date : 2022-12-12 DOI: 10.56557/joban/2022/v14i17960
U. Devi, G. Odelu, M. Venkateshwarlu, T. Ugandhar
An effective technique for the stimulation of somatic embryogenesis and plantlet regeneration from Leaf explants was developed in the sponge gourd (Luffa aegyptiaca (Mill.), which is significant in the field of medicinal botany. Fast-growing, yellowish nodular callus lines containing somatic embryos were established on an initiation medium supplemented with 2.0 mg/L 2,4-D, 3.0 mg/L 2,4-5-T, and 2.0 mg/L NAA, respectively. Somatic embryos were induced directly from cotyledon explants on Murashige and Skoog's (MS) medium fortified with different concentrations of 2,4- Dichlorophenoxyacetic acid, 2,4,5-trich On MS medium that was supplemented with 2.0 mg/L2,4-D and 1.5 mg/L TDZ, as well as 3.0 mg/L2,4-D and 2.0 mg/L BAP, embryo growth and good maturation were successfully accomplished. On an MS medium containing 2.0 mg/L TDZ and 2.0 mg/L NAA (-Naphthalene acetic acid), the well-formed embryos germinated into full plantlets and became plantlets. NAA stands for -Naphthalene acetic acid. The plants that had been regenerated were first transplanted into plastic cups and then into pots for maturation. After that, they were moved to soil and developed to maturity with an 80% survival rate, and the tissue culture-reared plants produced viable seeds.
研究了一种在药用植物学领域具有重要意义的海绵葫芦(Luffa aegyptiaca, Mill.)叶片外植体诱导体细胞胚胎发生和植株再生的有效技术。在分别添加2.0 mg/L 2,4- d、3.0 mg/L 2,4-5- t和2.0 mg/L NAA的诱导培养基上,建立了快速生长的淡黄色结节愈伤组织。在添加不同浓度2,4-二氯苯氧乙酸、2,4,5-富的Murashige和Skoog (MS)培养基上直接诱导体胚,在添加2.0 mg/L2、4- d和1.5 mg/L TDZ以及3.0 mg/L2、4- d和2.0 mg/L BAP的MS培养基上,胚发育良好,成熟良好。在含有2.0 mg/L TDZ和2.0 mg/L NAA(-萘乙酸)的MS培养基上,形成良好的胚萌发成完整的植株并成为植株。NAA代表-萘乙酸。再生的植株首先被移栽到塑料杯中,然后移栽到花盆中成熟。然后移入土壤,以80%的成活率发育成熟,组织培养的植株产生了可活的种子。
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引用次数: 0
THE EFFECT OF N/P RATIO OF UREA AND TSP FERTILIZERS IN WALNE MEDIA ON THE GROWTH PATTERN OF AND PROTEIN CONTENT OF Thalassiosira Sp. DIATOM CELLS 尿素氮磷比和TSP对水藻(Thalassosira Sp.)硅藻细胞生长模式和蛋白质含量的影响
Pub Date : 2022-12-01 DOI: 10.56557/joban/2022/v14i17944
D. Chilmawati, .. Subandiyono, Tristiana Yuniarti
The availability of natural feed needed in hatchery business is very significant due to the fact that natural feed will help the hatchery process to produce good and hi-quality seeds. Thalassiosira sp. is a type of diatom phytoplankton widely used to feed shrimp larvae. Walne media is a medium commonly used in the process of phytoplankton culture. Nutrient has considerable effect on the biochemical composition of phytoplankton, such as the content of protein, amino acids, carbohydrates, fats, and fatty acids. Nitrates and phosphorus contained in water are the main nutrients for phytoplankton to produce chlorophyll. One of the fertilizers which can be used for phytoplankton culture is agricultural fertilizers such as urea and TSP. The aims of this study were (1) to examine the effect of the application of the N/P ratio of urea and TSP in Walne media on the growth pattern and protein content of Thalassiosira sp. diatom cells; and (2) to determine the best value of the N/P ratio of urea and TSP fertilizers in Walne media which provides growth patterns and protein content of diatom cells of Thalassiosira sp. The method used in this research was an experimental method. The experimental method used was a completely randomized design (CRD) with 5 treatments and 4 designs, each treatment was as follows: applying different N/P ratios of urea and TSP fertilizers in Walne media, i.e. (A) 1:1; (B) 4:1; (C) 8:1; (D) 12:1; and (E) 16:1. The results of this study showed that algae growth increased from 0:0 to 16:0 N:P ratio. The growth of diatoms with the highest density occurred in treatments A and B with cell densities of 2.255×106 to 2.256×106 cells/ml compared to the other N:P ratios. It can be concluded the 1:1 N/P ratio of urea and TSP fertilizers in Walne media had a significant effect on growth patterns and protein content of diatom cells of Thalassiosira sp.
孵化场业务所需的天然饲料的可用性非常重要,因为天然饲料将有助于孵化过程产生优质种子。Thalassiosila sp.是一种硅藻浮游植物,广泛用于喂养虾幼虫。Walne培养基是浮游植物培养过程中常用的一种培养基。营养物质对浮游植物的生化组成有相当大的影响,如蛋白质、氨基酸、碳水化合物、脂肪和脂肪酸的含量。水中所含的硝酸盐和磷是浮游植物产生叶绿素的主要营养物质。可用于浮游植物培养的肥料之一是农业肥料,如尿素和TSP。本研究的目的是:(1)研究在Walne培养基中施用尿素和TSP的N/P比对Thalassosira sp.硅藻细胞生长模式和蛋白质含量的影响;以及(2)确定尿素和TSP肥料在Walne培养基中的最佳N/P比,该培养基提供了Thalassiosila sp.硅藻细胞的生长模式和蛋白质含量。本研究中使用的方法是一种实验方法。所用的实验方法是完全随机设计(CRD),有5个处理和4个设计,每个处理如下:在Walne培养基中施用不同的尿素和TSP肥料N/P比,即(a)1:1;(B) 4:1;(C) 8:1;(D) 12:1;和(E)16:1。本研究结果表明,从0到16时,藻类生长量增加。最高密度的硅藻生长在处理A和B中,与其他氮磷比相比,细胞密度为2.255×106至2.256×106个细胞/ml。结果表明,尿素和TSP在Walne培养基中1:1的氮磷比对藻硅藻细胞的生长模式和蛋白质含量有显著影响。
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引用次数: 0
EXPRESSION OF ANTIGEN THOMSEN–FRIEDENREICH RECOGNIZED BY Amaranthus leucocarpus LECTIN IN MCF CELLS STIMULATED WITH LIPOPOLYSACCHARIDE 脂肪多糖刺激的MCF细胞中Amaranthus leucarpus LECTIN识别的THOMESEN-FRIEDENRICH抗原的表达
Pub Date : 2022-10-21 DOI: 10.56557/joban/2022/v14i17902
Luis Miguel García Cruz, Iván Antonio García Montalvo, Berenice Fernández Rojas, Itandehui Gallegos Velasco, Pedro Antonio Hernández Cruz
Altered glycosylation is a characteristic of cancer cells, where the overexpression of truncated antigens such as the Thomsen–Friedenreich (TF) antigen (Galβ1, 3GalNAcα1, O-Ser / Thr) The TF antigen is specifically recognized by the Amaranthus leucocarpus (ALL). The MCF-7 breast cancer cell line maintains characteristics similar to differentiated mammary epithelial cells. It has been linked to stimulation of breast cancer cells with lipopolysaccharide, with a higher risk of metastasis in this type of cancer. In this work the expression of TF antigen and moesin in MCF-7 stimulated with LPS at concentration of 20 ng / ml cells was determined, by flow cytometry and fluorescence microscopy. MCF-7 cells express 45% of TF antigen without stimulus with LPS, the expression of the TF antigen decreases with the time of LPS stimulation. Moesin expression increases with LPS stimulation time. A double-positive MCF-7 population for moesin and a TF-antigen of 6.34% were identified by cytometry at 6 hours of LPS stimulation, compared to cells with no stimulus, whose double positivity was 2.86%. Our results suggests that moesin could be Amaranthus leucocarpus receptor in MCF-7 cells.
糖基化改变是癌症细胞的特征,其中截短抗原如Thomsen–Friedenreich(TF)抗原(Galβ1,3GalNAcα1,O-Ser/Thr)的过度表达。MCF-7乳腺癌症细胞系保持与分化的乳腺上皮细胞相似的特征。它与脂多糖刺激癌症细胞有关,在这种类型的癌症中,脂多糖具有更高的转移风险。在本工作中,通过流式细胞术和荧光显微镜测定了在浓度为20ng/ml的LPS刺激的MCF-7细胞中TF抗原和moesin的表达。MCF-7细胞在没有LPS刺激的情况下表达45%的TF抗原,TF抗原的表达随着LPS刺激的时间而降低。Moesin的表达随着LPS刺激时间的延长而增加。在LPS刺激6小时时,通过细胞术鉴定出moesin的MCF-7双阳性群体和6.34%的TF抗原,而没有刺激的细胞的双阳性率为2.86%。我们的结果表明,moesin可能是MCF-7细胞中的阿玛珠白果受体。
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引用次数: 0
EFFECT OF SOLVENTS ON PHYTOCHEMICAL PROFILING BY GC-Ms ANALYSIS AND In vitro CYTOTOXICITY OF BEE PROPOLIS AGAINST LEUKEMIA AND LUNG CANCER CELL LINES 溶剂对GC-Ms分析植物化学谱的影响及蜂胶对白血病和肺癌细胞系的体外细胞毒性
Pub Date : 2022-05-19 DOI: 10.56557/joban/2022/v14i17686
Rajalaxmi Sahoo, V. Sivaram, M. Anusha
Propolis is well-known for its favourable biological properties, which are widely employed in the treatment of a variety of disorders. It is high in chemical components that are dependent on the sort of plant that bees may access. The chemical makeup of an Indian Propolis sample was investigated using GCMS, and 22 chemicals were discovered. The MTT assay was used to test the ethanol extract of Propolis against leukaemia and lung cancer cell lines in vitro. The propolis extract was incubated with cancer cell lines for 24 hours, cytotoxicity was assessed using a colorimetric method, and the IC50 value was obtained. The findings showed that the propolis extract tested had substantial cytotoxic action. The conclusion is that Indian Propolis has potential. The Indian Propolis was shown to be a possible natural source of chemopreventive agent. The chemical makeup of Indian Propolis suggests that it could be a viable source of novel bioactive chemicals, but the molecules responsible for biological activity must be identified.
蜂胶以其良好的生物特性而闻名,广泛用于治疗各种疾病。它的化学成分含量很高,这取决于蜜蜂可能接触到的植物种类。使用GCMS对印度蜂胶样品的化学成分进行了调查,发现了22种化学物质。采用MTT法检测蜂胶乙醇提取物对白血病细胞株和癌症细胞株的抑制作用。将蜂胶提取物与癌症细胞系孵育24小时,使用比色法评估细胞毒性,并获得IC50值。研究结果表明,所测试的蜂胶提取物具有显著的细胞毒性作用。结论是印度蜂胶具有一定的潜力。印度蜂胶被证明是一种可能的化学预防剂的天然来源。印度蜂胶的化学成分表明,它可能是新型生物活性化学物质的可行来源,但必须确定负责生物活性的分子。
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引用次数: 0
HEALTH STATUS OF STRAY DOGS IN HUMAN SOCIETY, THEIR POTENTIAL TO HARBOR VARIOUS FUNGAL PATHOGENS AND CALL FOR HUMANE METHOD OF MANAGEMENT 流浪狗在人类社会中的健康状况,其潜在的各种真菌病原体和呼吁人性化的管理方法
Pub Date : 2022-01-15 DOI: 10.56557/joban/2022/v14i17507
Poornima Sharma
Dogs harbor and transmit various zoonotic diseases, fungal dermatitis being most prevalent. Animals, especially dogs, being integral pivot of human society need to be analyzed more closely in terms of their social and biological effects on human life. The purpose of this study was to understand the scenario of fungal agents related to dermal infections in dogs from twenty-one locations of Jabalpur (India). In addition, the dog population characteristics including their health status and interaction with human were also evaluated. In year 2017, 1576 dogs and in year 2018, 1740 dogs were surveyed for this purpose. Majority of ailments observed were dermal diseases. It was found that frequency of dermatitis cases was highest (ca.80% in year 2017-2018) among contagious aliments and directly proportional to the density of dogs. Fungi were isolated from hair and scale samples from lesion periphery. Fungi, causing ailments ranging from mild local allergies to contagious cutaneous diseases, were isolated on Dermatophye Test Medium and Sabouraud Dextrose Agar medium. Non- dermatophytic moulds (44.6%) were isolated more frequently than dermatophytes (33.9%). Yeast forms (21.4%) were at third position in frequency of isolation. Aspergillus species (36%) were most frequent non- dermatophyte. Trichophyton species (68.4%) were the most frequently isolated dermatophyte; whereas, Malassezia species (50%) were prominent yeast forms. 47.5% of all isolates were positive for hair perforation test and 70.5% were positive for urease production test; whereas, 3.3% of isolates showed variable results for urease production test. In present research, position of stray dogs in human society in terms of animal welfare and ethical treatment has also been addressed.
狗携带和传播各种人畜共患疾病,真菌皮炎是最普遍的。动物,尤其是狗,作为人类社会不可或缺的枢纽,需要从它们对人类生活的社会和生物影响方面进行更密切的分析。本研究的目的是了解来自Jabalpur(印度)21个地点的狗的皮肤感染相关真菌剂的情况。此外,还评估了犬类的种群特征,包括它们的健康状况和与人的互动。2017年,1576只狗和2018年,1740只狗为此目的接受了调查。观察到的大多数疾病是皮肤疾病。结果发现,传染性食物中皮炎病例发生率最高(2017-2018年约为0.80%),且与犬密度成正比。从病变周围的毛发和鳞片样品中分离到真菌。真菌引起的疾病范围从轻微的局部过敏到传染性皮肤病,在皮肤菌试验培养基和Sabouraud葡萄糖琼脂培养基上分离。非皮肤真菌(44.6%)的分离率高于皮肤真菌(33.9%)。酵母菌(21.4%)在分离频率上排名第三。曲霉属(36%)是最常见的非皮癣菌。毛癣菌是分离最多的皮菌,占68.4%;而马拉色菌属(50%)是突出的酵母菌形式。发孔试验阳性占47.5%,脲酶生产试验阳性占70.5%;3.3%的分离株脲酶产酶试验结果不一致。在目前的研究中,流浪狗在动物福利和伦理待遇方面在人类社会中的地位也得到了解决。
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引用次数: 0
N-FATTY ACYLGLYCINES: UNDERAPPRECIATED ENDOCANNABINOID-LIKE FATTY ACID AMIDES? n -脂肪酰基甘氨酸:被低估的内源性大麻素样脂肪酸酰胺?
Pub Date : 2017-01-01 Epub Date: 2018-03-03
Ryan L Anderson, David J Merkler

Long-chain N-fatty acylglycines, R-CO-NH-CH2-COOH (where "R" refers to an unsaturated or saturated alkyl chain of at least 14 carbons) are found in mammals and insects and are structurally related to the cell-signaling, lipid-like, N-fatty acylethanolamines, R-CO-NH-CH2-CH2-OH (where "R" refers to an alkyl chain of at least 14 carbons). Accumulating evidence demonstrates that the N-fatty acylglycines have important cellular functions, but much work remains in order to fully appreciate and understand these biomolecules including: (a) more work on their functions in vivo, (b) measuring their concentrations in the cell, (c) defining the pathways for the biosynthesis and degradation, and (d) understanding the metabolic interconversion(s) between the N-fatty acylglycines and other fatty acid amides. The purpose of reviewing the current state-of-knowledge about the N-fatty acylglycines is to stimulate future research about this intriguing family of biomolecules.

长链n -脂肪酰基甘氨酸,R- co - nh - ch2 - cooh(其中“R”指至少14个碳的不饱和或饱和烷基链)存在于哺乳动物和昆虫中,在结构上与细胞信号传导类脂,n -脂肪酰基乙醇胺,R- co - nh - ch2 - ch2 - oh(其中“R”指至少14个碳的烷基链)相关。越来越多的证据表明,n -脂肪酰基甘氨酸具有重要的细胞功能,但为了充分认识和理解这些生物分子,还有很多工作要做,包括:(a)更多的研究它们在体内的功能,(b)测量它们在细胞中的浓度,(c)确定生物合成和降解的途径,以及(d)了解n -脂肪酰基甘氨酸与其他脂肪酸酰胺之间的代谢相互转化。回顾目前对n -脂肪酰基甘氨酸的认识,旨在促进对这一有趣的生物分子家族的进一步研究。
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引用次数: 0
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