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Brain Acetylcholinesterase Activity as a Potential Biomarker for the Rapid Assessment of Chlorpyrifos Toxicity in a Euryhaline Fish, Oreochromis mossambicus 脑乙酰胆碱酯酶活性作为快速评估毒死蜱对广盐鱼毒性的潜在生物标志物
Pub Date : 2008-07-31 DOI: 10.1080/15555270701779478
J. Rao
Acute studies of chlorpyrifos, O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate insecticide on fish, Oreochromis (Tilapia) mossambicus, were carried out to assess the toxicity in relation to the marker enzyme, acetylcholinesterase (AChE, EC 3.1.1.7) activity in a semi-static system. Chlorpyrifos can be rated as highly toxic to O. mossambicus, with median lethal concentrations (LC50) of 43.52, 35.89, 30.6 and, 25.78 μg L−1 for 24, 48, 72, and 96 h respectively. The time required for 50% inhibition (IT50) of acetylcholinesterase (AChE) activities were measured in the brain of O. mossambicus, exposed to different median lethal concentrations indicated a decrease in time as the concentration increased. After exposure, survival fish were transferred to clean (chemical free) water and studied. AChE recovery were recorded in regular intervals of 3, 7, 14 and 21 days as a biomarker of exposure to this compound. The fish exposed to the LC50 for 24 h (43.52 μg L−1) showed a significant inhibition of AChE ...
在半静态系统中,对毒死蜱O,O-二乙基-O-(3,5,6-三氯-2-吡啶基)磷硫代杀虫剂对罗非鱼(Oreochromis(罗非鱼)mossambicus)进行了急性毒性研究,以评估其与标记酶乙酰胆碱酯酶(AChE, EC 3.1.1.7)活性的关系。毒死蜱对mossambicus的中位致死浓度(LC50)分别为43.52、35.89、30.6和25.78 μg L−1,持续时间为24、48、72和96 h。测定了不同中位致死浓度下莫桑蠓脑内乙酰胆碱酯酶(AChE)活性50%抑制所需时间(IT50),结果表明,不同中位致死浓度下莫桑蠓脑乙酰胆碱酯酶活性50%抑制所需时间随中位致死浓度的增加而减少。暴露后,存活的鱼被转移到干净(无化学物质)的水中进行研究。每隔3、7、14和21天定期记录乙酰胆碱酯酶恢复情况,作为暴露于该化合物的生物标志物。LC50处理24 h (43.52 μ L−1),对乙酰胆碱酯酶(AChE)有显著抑制作用。
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引用次数: 11
Experimental Studies on the Bioaccumulation of Selected Heavy Metals and Radionuclides in the Blood Cockle Anadara granosa of the Bang Pakong Estuary 邦帕孔河口血蛤中重金属和放射性核素生物积累的实验研究
Pub Date : 2007-12-11 DOI: 10.1080/15555270701715977
K. Srisuksawad, N. Prasertchiewchan
Biokinetic experiments of essential metal (65Zn), heavy metals (51Cr, 203Hg) and anthropogenic radionuclides (57Co, 85Sr, and 134Cs) on Anadara granosa from Bang Pakong estuary were performed under controlled conditions (salinity 30 psu, temperature 28.5 ±1.5°C and pH 8.0 ±0.1). The results showed that A. granosa accumulates studied metals and radionuclides efficiently, except 85Sr and 134Cs. Uptake of 51Cr, 57Co and 65Zn in A. granosa displayed saturation kinetics with estimated whole-body steady-state concentration factors (CFss) of 24, 730, and 90, respectively. Accumulation of 203Hg by A. granosa followed a simple linear model (R2 = 0.83), with maximum concentration factor (CFM) of 166. Loss kinetics of 57Co, 65Zn and 203Hg were best described by a 2-component exponential model, with computed half lives for long-lived component ranging from 99 to 125 d, whereas loss kinetics of 51Cr was best described by a single exponential model with computed half life of 16 d. 51Cr, 57Co and 65Zn were distributed r...
在盐度30 psu、温度28.5±1.5°C、pH 8.0±0.1的控制条件下,对Bang Pakong河口的Anadara granosa进行了必需金属(65Zn)、重金属(51Cr、203Hg)和人为放射性核素(57Co、85Sr和134Cs)的生物动力学实验。结果表明,除85Sr和134Cs外,黄颡鱼对其他金属和放射性核素均有较强的富集能力。粗粒棘豆对51Cr、57Co和65Zn的吸收表现出饱和动力学,估计全身稳态浓度因子(CFss)分别为24、730和90。石竹对203Hg的积累符合简单线性模型(R2 = 0.83),最大浓度因子(CFM)为166。57Co、65Zn和203Hg的损失动力学最好用双组分指数模型来描述,长寿命组分的半衰期为99 ~ 125 d,而51Cr的损失动力学最好用单组分指数模型来描述,半衰期为16 d。
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引用次数: 3
Bioconcentration and Retention of 14C-hexachlorobenzene (HCB): II. The Estuarine Clam Mytella gyuanensis 14c -六氯苯(HCB)的生物富集与滞留:II。河口蚌Mytella gyuanensis
Pub Date : 2007-12-11 DOI: 10.1080/15555270701712776
M. Andréa, A. Tomás, Thaís M. Vampré, Oscar J.S. Barreto, L. C. Luchini
HCB (hexachlorobenzene) is a persistent organic pollutant still detected in marine environments and organisms. The ability of the estuarine clam Mytella guyanensis to take up 14C-HCB from the sediment and to retain the contaminant within its tissues was studied in estuarine experimental microcosms. The sediment, water and soft tissues of the clams were periodically sampled and analyzed for their radiocarbon, HCB and lipid contents. Although most of the radioactivity remained in the sediment, some was found dissolved in the water, and 14C-HCB residues increased in the clam tissues with time of exposure to the HCB-spiked sediment. Further to its bioaccumulation, HCB was distributed within the mussel tissues as extractable and bound residues, which remained associated to the soft tissues even after restoration of uncontaminated conditions. Although M. guyanensis lives and feeds buried into the sediment, HCB uptake in the mussel was strongly influenced by the levels of HCB occurring in the water column. Level...
六氯苯(HCB)是一种持久性有机污染物,在海洋环境和生物中仍然存在。在河口实验微环境中,研究了古yanmytella guyanensis从沉积物中吸收14C-HCB并将污染物保留在其组织内的能力。定期对蛤的沉积物、水和软组织进行采样,分析其放射性碳、六氯环己烷和脂质含量。虽然大部分放射性物质仍留在沉积物中,但也有一些被发现溶解在水中,随着接触含hcb的沉积物时间的延长,14C-HCB在蛤蜊组织中的残留量增加。除了生物积累外,六氯环己烷还以可提取和结合残留物的形式分布在贻贝组织中,即使在未受污染的条件下恢复,这些残留物仍与软组织有关。虽然古yanensis生活和摄食埋在沉积物中,但贻贝对六氯环己烷的吸收受到水柱中六氯环己烷水平的强烈影响。水平……
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引用次数: 1
Radiotracer Techniques: A Unique Tool in Marine Ecotoxicological Studies 放射性示踪剂技术:海洋生态毒理学研究的独特工具
Pub Date : 2007-12-11 DOI: 10.1080/15555270701714822
M. Warnau, P. Bustamante
Environmental Bioindicators Publication details, including instructions for authors and subscription information: http://www.informaworld.com/smpp/title~content=t716100765 Radiotracer Techniques: A Unique Tool in Marine Ecotoxicological Studies Michel Warnau a; Paco Bustamante b a International Atomic Energy Agency Marine Environment Laboratories, Principality of Monaco b CRELA UMR 6217, University of La Rochelle La Rochelle, France
出版物细节,包括作者和订阅信息的说明:http://www.informaworld.com/smpp/title~content=t716100765放射性示踪剂技术:海洋生态毒理学研究的独特工具Michel Warnau A;Paco Bustamante b a国际原子能机构海洋环境实验室,摩纳哥公国b CRELA UMR 6217,法国拉罗谢尔大学
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引用次数: 31
Bioaccumulation of 137Cs, Zn and Cr[VI] in the Green Mussel Perna viridis: Influence of Salinity and Temperature 137Cs、Zn和Cr[VI]在绿贻贝体内的生物积累:盐度和温度的影响
Pub Date : 2007-12-11 DOI: 10.1080/15555270701693596
R. M. Qureshi, A. Mashiatullah, N. Yaqoob, P. Akhtar, F. Chaghtai, A. Jabbar, M. Warnau
Bioaccumulation of one radionuclide (137Cs) and two metal radiotracers (65Zn and 51Cr[VI]) was studied under controlled laboratory conditions in the green mussel Perna viridis in order to assess their usefulness as sentinel species for possible radioactive contamination originating from the nuclear facilities situated along the Karachi coast, Pakistan and the nearby Mumbai coast, India. The influence of two important external environmental factors (salinity and temperature) was also assessed. Three different temperatures (23, 28 and 32°C) and three salinity levels (25, 30 and 35 p.s.u.), which represent the range of temperature and salinity in marine coastal waters of Karachi, were selected. The results showed that both temperature and salinity affect bioaccumulation in P. viridis. Higher bioconcentration factors and uptake rates were found with the increase in temperature but they decreased with the increase in salinity levels. This indicates that the temperature and the salinity are key parameters to be...
在受控的实验室条件下,研究了绿贻贝(Perna viridis)中一种放射性核素(137Cs)和两种金属放射性示踪剂(65Zn和51Cr[VI])的生物积累,以评估它们作为哨兵物种对可能来自巴基斯坦卡拉奇海岸和附近印度孟买海岸的核设施的放射性污染的有效性。还评估了两个重要的外部环境因素(盐度和温度)的影响。选择了三种不同的温度(23、28和32°C)和三种盐度水平(25、30和35 p.s.u.),这代表了卡拉奇海洋沿海水域的温度和盐度范围。结果表明,温度和盐度对绿藻的生物积累均有影响。随着温度的升高,生物浓缩因子和吸收速率升高,但随着盐度的升高而降低。这表明温度和盐度是研究的关键参数。
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引用次数: 3
Use of Perna viridis as a Bioindicator of Paralytic Shellfish Toxins at Low Pyrodinium bahamense var compressum Density using a Radioreceptor Assay 利用放射线受体测定法,在低巴哈氏焦二酸盐压缩密度下,将紫藻作为麻痹性贝类毒素的生物指示剂
Pub Date : 2007-12-11 DOI: 10.1080/15555270701693612
E. Sombrito, M. Honrado, A. Véra, R. S. Tabbada, M. L. Ranada, Juan R. Relox, M. Tangonan
A radioassay method, i.e. the Receptor Binding Assay, was used for studying the uptake of Paralytic Shellfish Poisoning (PSP) toxins in the green bay mussel Perna viridis highly consumed in the Philippines. This method allowed working at low cell density of Pyrodinium bahamense var compressum (∼102-103 cells/L) representative of early stages of toxic algal blooms. The results indicated that within 16 hours toxic levels of PSP are reached in the tissues of  P. viridis, confirming the suitability of the green mussel as an indicator organism for paralytic shellfish toxicity in bivalves during the early stages of the bloom. Results also demonstrated that the weight-specific toxicity significantly increased with mussel size reduction. This method, based on the competition between the labeled and unlabeled toxin for the sodium channel receptor, offers better sensitivity than the mouse bioassay method. With an increasing amount of toxins in the sample, the amount of radiolabeled toxin binding with the receptor d...
一种放射性测定方法,即受体结合测定法,用于研究菲律宾大量食用的绿湾贻贝Perna viridis对麻痹性贝类中毒(PSP)毒素的吸收。该方法允许在低细胞密度的bahamense var compressum(~ 102-103个细胞/L)下工作,代表了有毒藻华的早期阶段。结果表明,在16小时内,绿贻贝的组织中PSP达到了毒性水平,证实了绿贻贝在水华初期作为双壳类麻痹性贝类毒性的指示生物的适用性。结果还表明,随着贻贝尺寸的减小,重量特异性毒性显著增加。该方法基于标记毒素和未标记毒素对钠通道受体的竞争,具有比小鼠生物测定法更高的灵敏度。随着样本中毒素的增加,与受体结合的放射性标记毒素的数量…
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引用次数: 2
Bioaccumulation of Dissolved Arsenic in the Oyster Crassostrea virginica: A Radiotracer Study 溶态砷在珍珠贝中的生物积累:放射性示踪剂研究
Pub Date : 2007-12-11 DOI: 10.1080/15555270701693570
M. Gómez-Batista, M. Metian, J. Teyssie, C. Alonso-Hernández, M. Warnau
In the framework of a program focusing on marine resource protection and management in the Caribbean, the objective of this work was to characterize As bioaccumulation in the common edible oyster Crassostrea virginica. Dissolved As (stable As  +  73As as a tracer) was taken up according to saturation kinetics for all tested exposure concentrations (2–10 μg l−1), and steady-state was reached rapidly within ∼1 week. A slight decrease in uptake efficiency was observed for the higher concentration tested. Whole-body depuration kinetics showed that 73As was lost according to double exponential depuration kinetics that were characterized by short-lived biological half-lives (Tb1/2s) of 0.5–0.9 d and by long-lived Tb1/2l of 8–16 d. No significant difference in 73As retention was found among different initial exposure concentrations of As. Overall, our results indicate that C. virginica bioaccumulates As proportionally to the ambient dissolved As concentration, and retains it with similar efficiency regardless of...
在加勒比地区海洋资源保护和管理项目的框架内,本研究的目的是表征普通食用牡蛎(Crassostrea virginica)中砷的生物积累。溶解的As(稳定的As + 73As作为示踪剂)根据饱和动力学在所有测试暴露浓度(2-10 μg l−1)下被吸收,并在约1周内迅速达到稳态。浓度越高,吸收效率越低。全身净化动力学表明,73As的损失符合双指数净化动力学,其特征是生物半衰期短(Tb1/2s)为0.5 ~ 0.9 d,生物半衰期长(Tb1/2l)为8 ~ 16 d。不同初始暴露浓度下,73As的保留量无显著差异。总的来说,我们的研究结果表明,处女花的生物积累与环境溶解的砷浓度成正比,并且无论环境如何,其保持效率都相似。
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引用次数: 9
Bioaccumulation and Retention of 14C-hexachlorobenzene (HCB): I. The Marine Tropical Mussel Perna perna 14c -六氯苯(HCB)的生物积累和滞留:I.海洋热带贻贝
Pub Date : 2007-12-11 DOI: 10.1080/15555270701712768
M. Andréa, A. Tomás, Thaís M. Vampré, Oscar J.S. Barreto, L. C. Luchini
HCB (hexachlorobenzene) is a ubiquitous pollutant, which is highly toxic to aquatic organisms; however, it is continuously generated and released to the environment. In order to explore the potential of the mussel Perna perna as a sentinel species for monitoring HCB contamination, we have investigated the accumulation and depuration kinetics of 14C-HCB in the mussel exposed to spiked sediment. The sediment, water and mussels were sampled periodically and submitted to solvent extraction for the determination of the radiocarbon and organism lipid content. The analysis showed that most of the radioactivity remained in the sediment as a potential long-term source of pollution, whereas the HCB concentration in the seawater was below the detection limit. The mussel soft tissues were contaminated very little; the residues were distributed as extractable and bound residues, which were strongly correlated with the lipid content of the mussel tissues. The sensitive radiotracer technique used allowed the detection o...
六氯苯(HCB)是一种普遍存在的污染物,对水生生物具有剧毒;然而,它不断地产生并释放到环境中。为了探索永久贻贝作为监测HCB污染的哨兵物种的潜力,我们研究了暴露于尖刺沉积物的贻贝中14C-HCB的积累和净化动力学。沉积物、水和贻贝定期取样,并进行溶剂萃取,测定放射性碳和生物脂含量。分析表明,大部分放射性物质作为潜在的长期污染源留在沉积物中,而海水中的六氯环己烷浓度低于检测限。贻贝的软组织受到的污染很少;这些残基以可提取残基和结合残基的形式分布,与贻贝组织的脂质含量密切相关。使用灵敏的放射性示踪技术可以探测到……
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引用次数: 4
Monitoring of Exposure to and Potential Effects of Contaminants in the Environment 监测环境中污染物的暴露和潜在影响
Pub Date : 2007-09-25 DOI: 10.1080/15555270701603751
J. Giesy, J. Newsted
In our lifetimes, much of what was once considered science fiction: space ships, monitoring the environment from space, satellite phones and biomedical advances in the diagnosis and treatment of disease at the molecular level have now become realities. These advances in technology have changed our perceptions and how we interact with the world around us. Also during this time, the world population has doubled several times, our use of natural resources has increased exponentially and we continue to release natural and synthetic compounds to the global environment at an ever-increasing rate. These actions have altered the face of the planet in a multitude of ways. In the ultimate irony, technology has proven to be a double-edged sword that can both threaten to destroy us while also possibly providing the means of our salvation, our means of achieving sustainable development. How can we harness the rapidly developing technological means at our disposal to predict and manage environmental changes? There are exciting changes in technology that may provide the ability to monitor the environment, providing the information we need to allow us to make wise environmental policy decisions. However, even with these advances in technology, the basic dilemma still facing environmental scientists is complexity of ecosystems where known and unknown natural and anthropogenic factors may adversely influence natural processes, resulting in degradation of living resources, environmental services, and human health. Conversely, failure to effectively monitor will lead to our failure to detect threats to human health and reductions in biodiversity, resulting in higher costs associated with after-the-fact remediation and restoration, with the ultimate risk of irreversible damage to environmental resources. Within this context, it is important that as environmental scientists, we provide the necessary information to the general public and regulatory stakeholders that is readily interpretable and is related to valued resources and functions of ecosystems, allowing these stakeholder groups to make informed and effective resource-management decisions in real-time. Efforts are still needed to develop programs to detect, monitor and assess impacts in bio-diverse ecosystems by measuring the right things, in the right places, at the right frequency over sufficient time periods in a cost-effective manner. To increase the efficiency and effectiveness of monitoring programs focusing on “known” chemicals and the potential effects and risks they may pose to biota, including humans, we propose that risk-based monitoring programs be developed incorporating chemical and biological techniques that are rapid, readily implemented, and provide
在我们的有生之年,许多曾经被认为是科幻小说的东西:宇宙飞船、从太空监测环境、卫星电话,以及在分子水平上诊断和治疗疾病的生物医学进步,现在都变成了现实。这些技术进步改变了我们的观念以及我们与周围世界互动的方式。同样在这段时间里,世界人口翻了几倍,我们对自然资源的使用呈指数增长,我们继续以不断增长的速度向全球环境释放天然和合成化合物。这些活动以多种方式改变了地球的面貌。最具讽刺意味的是,技术已被证明是一把双刃剑,它既可能毁灭我们,也可能为我们提供救赎的手段,我们实现可持续发展的手段。我们如何利用迅速发展的技术手段来预测和管理环境变化?技术上的一些令人兴奋的变化可能会提供监测环境的能力,提供我们需要的信息,使我们能够做出明智的环境政策决定。然而,即使有了这些技术进步,环境科学家仍然面临的基本困境是生态系统的复杂性,其中已知和未知的自然和人为因素可能对自然过程产生不利影响,导致生物资源、环境服务和人类健康的退化。相反,不进行有效监测将导致我们无法发现对人类健康的威胁和生物多样性的减少,导致事后补救和恢复的费用更高,最终有可能对环境资源造成不可逆转的损害。在这种背景下,作为环境科学家,我们向公众和监管利益相关者提供必要的信息是很重要的,这些信息很容易解释,并且与生态系统的宝贵资源和功能有关,允许这些利益相关者群体实时做出明智和有效的资源管理决策。我们仍然需要努力制定计划,通过在适当的地点、以适当的频率、以具有成本效益的方式,在足够的时间内测量正确的事物,来检测、监测和评估对生物多样性生态系统的影响。为了提高以“已知”化学物质及其对生物群(包括人类)的潜在影响和风险为重点的监测项目的效率和有效性,我们建议开发基于风险的监测项目,结合化学和生物技术,这些技术可以快速、容易地实施,并提供有效的信息
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引用次数: 3
A Dedication to the Memory of Eliška Nováková (1921–2000), One of the Founders of the Study of Environmental Bioindicators 纪念环境生物指标研究奠基人之一Eliška Nováková (1921-2000
Pub Date : 2007-09-25 DOI: 10.1080/15555270701626471
J. Boháč, J. Newman
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引用次数: 0
期刊
Environmental bioindicators
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